Lcy04g003030 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g003030
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	vacuolar protein sorting-associated protein 18 homolog
Location	Chr04: 23808882 .. 23810102 (+)
RNA-Seq Expression	Lcy04g003030
Synteny	Lcy04g003030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACTGCCAAAAGTAGGAACCGTCCAAATGAAATCGCCAATTGAAAAAGACAGTCACAGAAAAGGTCCAAAACTGAAGAACTAAAGTAGTCGGACTAGGGGTACTGCATTTAACTTAAATGATTTAAGGCTGTTCAGGTTGTGGGTAGTCGCAGAAAGCCAAGATTAAGTCCAAATGAATGACTTCAACATCAAATAAGAATGAAAGTTATATTTTGTTTAGGATGTTGGGTTTGCTTTTGTAAGAGGATCATGATCAATCTAACATTGTTATATATAGGACCATATGGTCCTGATACAAGATATTTTCAGTGTATAGGTCCATATGAAATTGAATGAACTCTATATTTCCTTTTAACTAATTATAAAATGATGTTGACCATGCACATAATATTTTACTACTGGTAGCCTAAAATCTCTGCTTGTATATTTGGAGTTTCAATGACCTCTAGCAGCCACTTAAAAATTCTTGACATATCTATGCAGGAGGCAATTTGCTCATCATTAGAAGATTACAACAAGCAAATTGATCAGCTGAAACAGGACATGAATGATGCAACGCATGTTGCTGATAATATTAGGAAAGATATCAATGATCTTGCTCAAAGATATGCTGTAATTGATCGAGATGAAGATTGTGAGGTATTGATCATTCTAGTGCTAGCTGATAGCATTTTCCATTTCTTCAACTTGAGCCTTAGAAGCCACATAAACGAGTATAAAATTTTATACAAGATTACTAGTTGGGATTGCACAAGAATTATCTAATTTCTAATTGGGCTATCTAGTATTCTATACATTTTTTAGAGAGAATTTTAAAACTCAATATTTGATGTTCTTAAATCTTAATAAGGTGTATGGAGATGGTGTCTCCTTTCTCTCGAAATGAAGTGTAGGGTGATGGTGCAGATGTATGTTGTAATTATGGATATGGATAGAAGCAAATAAAATGTTGGCGAATAAAGAATGCCAATCCTGCAAATTCGGAGCTGATAAAGATTGGGTATCTCCAACCTAACGTCTATAGAGGGTGGATCACATACCGGAACGAATTCGAGGTCCAATTCGGACGCTGGAAAATGCGAGAACGATGTGTTTCCTTGCCGGAATCGCGTGGGCTGAAGTTGCACCTACAGAAGCGGAACTTGTAAGCCGCGGAGACTTAAAAGGAAACGGAAATTTTGGGGGAAAATTGCTTATCACCAAAGAACTCGAGAAAGTCA

mRNA sequence

CACTGCCAAAAGTAGGAACCGTCCAAATGAAATCGCCAATTGAAAAAGACAGTCACAGAAAAGGTCCAAAACTGAAGAACTAAAGTAGTCGGACTAGGGGTACTGCATTTAACTTAAATGATTTAAGGCTGTTCAGGTTGTGGGTAGTCGCAGAAAGCCAAGATTAAGTCCAAATGAATGACTTCAACATCAAATAAGAATGAAAGTTATATTTTGTTTAGGATGTTGGGTTTGCTTTTGTAAGAGGATCATGATCAATCTAACATTGTTATATATAGGACCATATGGTCCTGATACAAGATATTTTCAGTGTATAGGTCCATATGAAATTGAATGAACTCTATATTTCCTTTTAACTAATTATAAAATGATGTTGACCATGCACATAATATTTTACTACTGGTAGCCTAAAATCTCTGCTTGTATATTTGGAGTTTCAATGACCTCTAGCAGCCACTTAAAAATTCTTGACATATCTATGCAGGAGGCAATTTGCTCATCATTAGAAGATTACAACAAGCAAATTGATCAGCTGAAACAGGACATGAATGATGCAACGCATGTTGCTGATAATATTAGGAAAGATATCAATGATCTTGCTCAAAGATATGCTGTAATTGATCGAGATGAAGATTGTGAGGTATTGATCATTCTAGTGCTAGCTGATAGCATTTTCCATTTCTTCAACTTGAGCCTTAGAAGCCACATAAACGAGTATAAAATTTTATACAAGATTACTAGTTGGGATTGCACAAGAATTATCTAATTTCTAATTGGGCTATCTAGTATTCTATACATTTTTTAGAGAGAATTTTAAAACTCAATATTTGATGTTCTTAAATCTTAATAAGGTGTATGGAGATGGTGTCTCCTTTCTCTCGAAATGAAGTGTAGGGTGATGGTGCAGATGTATGTTGTAATTATGGATATGGATAGAAGCAAATAAAATGTTGGCGAATAAAGAATGCCAATCCTGCAAATTCGGAGCTGATAAAGATTGGGTATCTCCAACCTAACGTCTATAGAGGGTGGATCACATACCGGAACGAATTCGAGGTCCAATTCGGACGCTGGAAAATGCGAGAACGATGTGTTTCCTTGCCGGAATCGCGTGGGCTGAAGTTGCACCTACAGAAGCGGAACTTGTAAGCCGCGGAGACTTAAAAGGAAACGGAAATTTTGGGGGAAAATTGCTTATCACCAAAGAACTCGAGAAAGTCA

Coding sequence (CDS)

ATGACCTCTAGCAGCCACTTAAAAATTCTTGACATATCTATGCAGGAGGCAATTTGCTCATCATTAGAAGATTACAACAAGCAAATTGATCAGCTGAAACAGGACATGAATGATGCAACGCATGTTGCTGATAATATTAGGAAAGATATCAATGATCTTGCTCAAAGATATGCTGTAATTGATCGAGATGAAGATTGTGAGGTATTGATCATTCTAGTGCTAGCTGATAGCATTTTCCATTTCTTCAACTTGAGCCTTAGAAGCCACATAAACGAGTATAAAATTTTATACAAGATTACTAGTTGGGATTGCACAAGAATTATCTAA

Protein sequence

MTSSSHLKILDISMQEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEVLIILVLADSIFHFFNLSLRSHINEYKILYKITSWDCTRII

Homology

[Close]

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot
Match: F4IDS7 (Vacuolar sorting protein 18 OS=Arabidopsis thaliana OX=3702 GN=VPS18 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.1e-16
Identity = 42/54 (77.78%), Postives = 48/54 (88.89%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQI+QLK++MNDAT  ADNIR DI+ L QRYAVIDRDE+C V
Sbjct: 785 KEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGV 838

[Close]

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot
Match: P59015 (Vacuolar protein sorting-associated protein 18 homolog OS=Danio rerio OX=7955 GN=vps18 PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-08
Identity = 41/115 (35.65%), Postives = 61/115 (53.04%), Query Frame = 0


Query: 1   MTSSSHLKILDI-----------SMQEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKD 60
           ++S + LKI DI             +EAICSSLE+YNK I++LKQ+M +AT  A  IR+D
Sbjct: 777 LSSCNLLKIEDILPFFPDFVTIDHFKEAICSSLEEYNKHIEELKQEMEEATESAKRIRED 836

Query: 61  INDLAQRYAVIDRDEDCEVL--------IILVLADSIFHFFNL--SLRSHINEYK 95
           I ++  +Y V++  E C             L L   +FH+  L   +  H++ YK
Sbjct: 837 IQEMRNKYGVVESQEKCATCDFPLLNRPFYLFLCGHMFHYDCLLQEVIPHLSVYK 891

[Close]

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot
Match: Q9P253 (Vacuolar protein sorting-associated protein 18 homolog OS=Homo sapiens OX=9606 GN=VPS18 PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 3.2e-05
Identity = 22/52 (42.31%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDC 67
           +EAICSSL+ YN  I +L+++M +AT  A  IR+D+ +L  RY  ++  + C
Sbjct: 802 KEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKC 853

[Close]

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot
Match: Q8R307 (Vacuolar protein sorting-associated protein 18 homolog OS=Mus musculus OX=10090 GN=Vps18 PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 3.2e-05
Identity = 22/52 (42.31%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDC 67
           +EAICSSL+ YN  I +L+++M +AT  A  IR+D+ +L  RY  ++  + C
Sbjct: 802 KEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKC 853

[Close]

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot
Match: Q24314 (Vacuolar protein sorting-associated protein 18 homolog OS=Drosophila melanogaster OX=7227 GN=dor PE=1 SV=3)

HSP 1 Score: 48.5 bits (114), Expect = 5.5e-05
Identity = 25/96 (26.04%), Postives = 49/96 (51.04%), Query Frame = 0


Query: 13  SMQEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEVLIIL 72
           + +EAIC +L DYN++I +L+++M + T   D +  ++  L Q    ++  + CE+  ++
Sbjct: 832 NFKEAICDALRDYNQRIQELQREMAETTEQTDRVTAELQQLRQHSLTVESQDTCEICEMM 891

Query: 73  VLADSIFHFF-NLSLRSHINEYKILYKITSWDCTRI 108
           +L    F F       S   E  ++  +T   C R+
Sbjct: 892 LLVKPFFIFICGHKFHSDCLEKHVVPLLTKEQCRRL 927

[Close]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL
Match: A0A6J1HZJ9 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita maxima OX=3661 GN=LOC111468106 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYA+IDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL
Match: A0A6J1GKZ1 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454916 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYA+IDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL
Match: A0A5A7SQQ7 (Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003670 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.8e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAIC+SLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYAVIDRDEDC V
Sbjct: 797 KEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGV 850

[Close]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL
Match: A0A5D3C570 (Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003690 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.8e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAIC+SLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYAVIDRDEDC V
Sbjct: 704 KEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGV 757

[Close]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL
Match: A0A0A0K5N6 (Pep3_Vps18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046700 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.8e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAIC+SLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYAVIDRDEDC V
Sbjct: 785 KEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. NCBI nr
Match: XP_038887715.1 (vacuolar sorting protein 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 98.6 bits (244), Expect = 3.5e-17
Identity = 49/54 (90.74%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYAVIDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. NCBI nr
Match: XP_038887716.1 (vacuolar sorting protein 18 isoform X2 [Benincasa hispida])

HSP 1 Score: 98.6 bits (244), Expect = 3.5e-17
Identity = 49/54 (90.74%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYAVIDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. NCBI nr
Match: XP_023511650.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.2 bits (243), Expect = 4.6e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYA+IDRDEDC V
Sbjct: 773 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGV 826

[Close]

BLAST of Lcy04g003030 vs. NCBI nr
Match: XP_022968943.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita maxima])

HSP 1 Score: 98.2 bits (243), Expect = 4.6e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYA+IDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. NCBI nr
Match: KAG6572340.1 (Vacuolar sorting protein 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.2 bits (243), Expect = 4.6e-17
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQIDQLKQ+MNDATH ADNIRKDIN LAQRYA+IDRDEDC V
Sbjct: 785 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGV 838

[Close]

BLAST of Lcy04g003030 vs. TAIR 10
Match: AT1G12470.1 (zinc ion binding )

HSP 1 Score: 85.9 bits (211), Expect = 2.2e-17
Identity = 42/54 (77.78%), Postives = 48/54 (88.89%), Query Frame = 0


Query: 15  QEAICSSLEDYNKQIDQLKQDMNDATHVADNIRKDINDLAQRYAVIDRDEDCEV 69
           +EAICSSLEDYNKQI+QLK++MNDAT  ADNIR DI+ L QRYAVIDRDE+C V
Sbjct: 785 KEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGV 838

The following BLAST results are available for this feature:

BLAST of Lcy04g003030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IDS7	3.1e-16	77.78	Vacuolar sorting protein 18 OS=Arabidopsis thaliana OX=3702 GN=VPS18 PE=1 SV=1	[more]
P59015	1.4e-08	35.65	Vacuolar protein sorting-associated protein 18 homolog OS=Danio rerio OX=7955 GN...	[more]
Q9P253	3.2e-05	42.31	Vacuolar protein sorting-associated protein 18 homolog OS=Homo sapiens OX=9606 G...	[more]
Q8R307	3.2e-05	42.31	Vacuolar protein sorting-associated protein 18 homolog OS=Mus musculus OX=10090 ...	[more]
Q24314	5.5e-05	26.04	Vacuolar protein sorting-associated protein 18 homolog OS=Drosophila melanogaste...	[more]

BLAST of Lcy04g003030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZJ9	2.2e-17	88.89	vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita maxima OX=36...	[more]
A0A6J1GKZ1	2.2e-17	88.89	vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita moschata OX=...	[more]
A0A5A7SQQ7	3.8e-17	88.89	Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var....	[more]
A0A5D3C570	3.8e-17	88.89	Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var....	[more]
A0A0A0K5N6	3.8e-17	88.89	Pep3_Vps18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046700 ...	[more]

BLAST of Lcy04g003030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887715.1	3.5e-17	90.74	vacuolar sorting protein 18 isoform X1 [Benincasa hispida]	[more]
XP_038887716.1	3.5e-17	90.74	vacuolar sorting protein 18 isoform X2 [Benincasa hispida]	[more]
XP_023511650.1	4.6e-17	88.89	vacuolar protein sorting-associated protein 18 homolog [Cucurbita pepo subsp. pe...	[more]
XP_022968943.1	4.6e-17	88.89	vacuolar protein sorting-associated protein 18 homolog [Cucurbita maxima]	[more]
KAG6572340.1	4.6e-17	88.89	Vacuolar sorting protein 18, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lcy04g003030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12470.1	2.2e-17	77.78	zinc ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 15..56
None	No IPR available	PANTHER	PTHR23323	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN	coord: 13..77
None	No IPR available	PANTHER	PTHR23323:SF27	BNACNNG33440D PROTEIN	coord: 13..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g003030.1	Lcy04g003030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007032	endosome organization
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0048284	organelle fusion
biological_process	GO:0010015	root morphogenesis
biological_process	GO:0007033	vacuole organization
biological_process	GO:0006904	vesicle docking involved in exocytosis
cellular_component	GO:0033263	CORVET complex
cellular_component	GO:0030897	HOPS complex
cellular_component	GO:0009705	plant-type vacuole membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030674	protein-macromolecule adaptor activity