Lcy04g002740 (gene) Sponge gourd (P93075) v1

BLAST of Lcy04g002740 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.6e-46
Identity = 103/281 (36.65%), Postives = 142/281 (50.53%), Query Frame = 0

Query: 14  LLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREPTHITLAKMGDVYGPIF 73
           L+F   LF         G  +  P  GGAWP+ GHL  L   +  H  L  + + +GP+F
Sbjct: 18  LIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGPLF 77

Query: 74  TLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWRHV 133
           T+KLG   AL+VS WE+A+ECFTTND   ++RPK+L +EL+ YN A + V+PY PYWR +
Sbjct: 78  TIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWREL 137

Query: 134 RKIATLELRTNHRLEQLQHIRISE------------------------------------ 193
           RKI   E+ ++ R+EQLQ +R+SE                                    
Sbjct: 138 RKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFN 197

Query: 194 -----------------------------DLLVLFGAFVPSDSFPFLDWFDLGGYKKAMK 230
                                        + + L G F   D+ P+L W D GGY+KAMK
Sbjct: 198 MVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAMK 257

BLAST of Lcy04g002740 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.0e-45
Identity = 108/290 (37.24%), Postives = 149/290 (51.38%), Query Frame = 0

Query: 7   LAASFALLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREPTHITLAKMG 66
           +A +  L L F  LF + R+ SR GK    P   GAWP++GHL  L+  +  H  L  + 
Sbjct: 13  IAIASILSLIFLCLF-LYRKNSR-GKD--APVVSGAWPILGHLSLLNGSQTPHKVLGALA 72

Query: 67  DVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPY 126
           D YGP+FT+KLG   AL++S+WE++KE FTTND   +SRPK++A E++ YN A +G++PY
Sbjct: 73  DKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPY 132

Query: 127 DPYWRHVRKIATLELRTNHRLEQLQHIRISE----------------------------- 186
            PYWR +RKI T E  +N R+EQ  HIR+SE                             
Sbjct: 133 GPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQ 192

Query: 187 ---------------------------------------DLLVLFGAFVPSDSFPFLDWF 229
                                                  + + L G F  +D  P L W 
Sbjct: 193 WLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWL 252

BLAST of Lcy04g002740 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.7e-45
Identity = 107/294 (36.39%), Postives = 149/294 (50.68%), Query Frame = 0

Query: 3   LAQVLAASFALL-LFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREPTHIT 62
           L  +L   F LL  FFY ++          K + PP+  GAWP+IGHLH L+  +  H  
Sbjct: 7   LETLLLGFFVLLPCFFYFVWKKPN-----NKIKEPPQPAGAWPIIGHLHLLARGDLPHKI 66

Query: 63  LAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASM 122
           L+   D  GP+F ++LG + AL+V++ EIAKECFTTNDR F +RP  +A++++GYNY  +
Sbjct: 67  LSSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVML 126

Query: 123 GVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE------------------------ 182
           GV+PY PYWR +RKI  LE  +N RL+ L+H+  SE                        
Sbjct: 127 GVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDM 186

Query: 183 -------------------------------------------DLLVLFGAFVPSDSFPF 229
                                                      ++  L G+FV SD  P+
Sbjct: 187 KQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPY 246

BLAST of Lcy04g002740 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 1.1e-44
Identity = 108/306 (35.29%), Postives = 143/306 (46.73%), Query Frame = 0

Query: 9   ASFALLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREPTHITLAKMGDV 68
           A  +L+   + LF    RFS+    + PP   G+WP++GHL  +   +  H TL  + D 
Sbjct: 12  AFISLISLLFFLF----RFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALVDK 71

Query: 69  YGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDP 128
           YGPIFT+KLG   AL++S+WE+AKECFT ND   +SRPK +A EL+ YN A +G +PY  
Sbjct: 72  YGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGA 131

Query: 129 YWRHVRKIATLELRTNHRLEQLQHIRISE------------------------------- 188
           YWR +RKI TLE+ +N R+E L HIR+SE                               
Sbjct: 132 YWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSST 191

Query: 189 -------------------------------------------------------DLLVL 228
                                                                  D + L
Sbjct: 192 NDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRL 251

BLAST of Lcy04g002740 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.1e-44
Identity = 107/286 (37.41%), Postives = 149/286 (52.10%), Query Frame = 0

Query: 13  LLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREP-THITLAKMGDVYGP 72
           L+  F ALF  S++     K    P   GAWP+IGHLH L  +E   + TL KM D YGP
Sbjct: 13  LVFVFIALFKKSKK----PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGP 72

Query: 73  IFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWR 132
             +L+LG+N A +VSS+E+AK+CFT ND+  ASRP   A++ +GYN+A  G +PY  +WR
Sbjct: 73  AMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWR 132

Query: 133 HVRKIATLELRTNHRLEQLQHIRISE---------------------------------- 192
            +RKIAT+EL +N RL+ L+H+R+SE                                  
Sbjct: 133 EMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTL 192

Query: 193 DLLV-----------------------------------LFGAFVPSDSFPFLDWFDLGG 229
           +++V                                   L G F  SD+FP L +FDL G
Sbjct: 193 NMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQG 252

BLAST of Lcy04g002740 vs. ExPASy TrEMBL
Match: A0A6J1FBD1 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442568 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 3.1e-85
Identity = 179/292 (61.30%), Postives = 192/292 (65.75%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           MEL  VL A FALLLF YALF+I+ R S   +  KRLPPEAGG  PVIGHLHKLSA EPT
Sbjct: 1   MELTHVLPAIFALLLFLYALFAITSRRSSAHRTNKRLPPEAGGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASR K +ASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDSIFASRSKAVASELLGYNY 120

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
             + +SPY PYWRHVRKIATLEL TNHRL QLQHIRISE                     
Sbjct: 121 TVLSLSPYGPYWRHVRKIATLELLTNHRLHQLQHIRISEVRSSIKKLYELNKGSGGKVRV 180

Query: 181 ----------------------------------------DLLVLFGAFVPSDSFPFLDW 229
                                                   DLL L GAFVPSDSFPFL W
Sbjct: 181 EMRTWFGDATLNTIFRMVMGKRFSTVFEGSGGEQYRQALRDLLELLGAFVPSDSFPFLSW 240

BLAST of Lcy04g002740 vs. ExPASy TrEMBL
Match: A0A6J1F723 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.7e-83
Identity = 175/299 (58.53%), Postives = 191/299 (63.88%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRC---GKKRLPPEAGGAWPVIGHLHKLSAREP 60
           MEL  +L A+FALLLF YALF I+ R S       KR PPEA GA P+IGHLHKLSA EP
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYN 120
           THITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASR K++ASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE-------------------- 180
           YA++G+SPY PYWRHVRK+ATLEL TNHRLEQLQHIRI E                    
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 ----------------------------------------------DLLVLFGAFVPSDS 229
                                                             LF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

BLAST of Lcy04g002740 vs. ExPASy TrEMBL
Match: A0A6J1F6F5 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442560 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.1e-82
Identity = 177/293 (60.41%), Postives = 188/293 (64.16%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           MEL   + A+FALLLF YALF IS R S   +    LPPEAGG  PVIGHLHKLSA EPT
Sbjct: 41  MELTHNIPATFALLLFLYALFRISSRRSAAHRTNNTLPPEAGGRLPVIGHLHKLSATEPT 100

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASR K LASELLGYNY
Sbjct: 101 HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKGLASELLGYNY 160

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
             + VSPY PYWRHVRKIAT EL  N RLEQLQHIR+SE                     
Sbjct: 161 TLLSVSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKKLYELNKGSGGKVRV 220

Query: 181 ----------------------------------------DLLVLFGAFVPSDSFPFLDW 229
                                                   D+  LFGAFVPSDSFP L W
Sbjct: 221 EMRRWFGDVSLNTILRMVVGKRLSSVFEGSGAEQYKEALRDVFELFGAFVPSDSFPCLSW 280

BLAST of Lcy04g002740 vs. ExPASy TrEMBL
Match: A0A6J1IF60 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476389 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.4e-82
Identity = 174/298 (58.39%), Postives = 190/298 (63.76%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           ME+  +L A FALLL  YALF I+   S   +  K LPPEA G  P+IGHLHKLSA EPT
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAANRTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM DVYGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
           A M +SPY PYWRHVRK+ATLEL TNHRLEQLQHIRISE                     
Sbjct: 121 AMMAISPYGPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---------------------------------------------DLLVLFGAFVPSDSF 229
                                                        D+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRKWFGDVSLNTIFRMVVGKRFSAVFEGSGGEQYRRALRDVFELFGAFVPSDSF 240

BLAST of Lcy04g002740 vs. ExPASy TrEMBL
Match: A0A6J1F6A2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442569 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.4e-82
Identity = 174/294 (59.18%), Postives = 190/294 (64.63%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRR--FSRCGKKRLPPEAGGAWPVIGHLHKLSAREPT 60
           ME     AA+FALLLF YALF I+ R   +      LPPEAGGA P+IGHLHKLSA +P 
Sbjct: 1   MEHTHSSAATFALLLFLYALFRIASRRPGAHSRNNTLPPEAGGALPLIGHLHKLSATQPP 60

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTL+LG N ALIVSSWEIA+ECFTTND  FASRPK LASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNKALIVSSWEIARECFTTNDSIFASRPKGLASELLGYNY 120

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
             + +SPY PYWR VRKIATLEL TNHRL QLQHIRISE                     
Sbjct: 121 TVLSISPYGPYWRRVRKIATLELLTNHRLHQLQHIRISEVQSSIKKLYELNKGSGGRVRV 180

Query: 181 ----------------------------------------DLLVLFGAFVPSDSFPFLDW 230
                                                   DL  LFGAFVPSDSFPFL W
Sbjct: 181 EMRAWFGDVSLNTIFRMVVGKRFSTVFEGSGAEQYREAFRDLSELFGAFVPSDSFPFLSW 240

BLAST of Lcy04g002740 vs. NCBI nr
Match: XP_022935743.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 324.7 bits (831), Expect = 6.4e-85
Identity = 179/292 (61.30%), Postives = 192/292 (65.75%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           MEL  VL A FALLLF YALF+I+ R S   +  KRLPPEAGG  PVIGHLHKLSA EPT
Sbjct: 1   MELTHVLPAIFALLLFLYALFAITSRRSSAHRTNKRLPPEAGGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASR K +ASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDSIFASRSKAVASELLGYNY 120

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
             + +SPY PYWRHVRKIATLEL TNHRL QLQHIRISE                     
Sbjct: 121 TVLSLSPYGPYWRHVRKIATLELLTNHRLHQLQHIRISEVRSSIKKLYELNKGSGGKVRV 180

Query: 181 ----------------------------------------DLLVLFGAFVPSDSFPFLDW 229
                                                   DLL L GAFVPSDSFPFL W
Sbjct: 181 EMRTWFGDATLNTIFRMVMGKRFSTVFEGSGGEQYRQALRDLLELLGAFVPSDSFPFLSW 240

BLAST of Lcy04g002740 vs. NCBI nr
Match: XP_023535446.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.7 bits (818), Expect = 2.0e-83
Identity = 177/298 (59.40%), Postives = 191/298 (64.09%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           MEL  +L A+FALLLF YALF+I+ R S   +    LPPE  G  P+IGHLHKLSA EPT
Sbjct: 1   MELTHILPATFALLLFLYALFAITSRRSAAHRTNNTLPPEPAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADAYGPIFTLRLGVNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
           A M +SPY PYWRHVRKIATLEL TNHRLEQLQHIRISE                     
Sbjct: 121 AMMAISPYGPYWRHVRKIATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---------------------------------------------DLLVLFGAFVPSDSF 229
                                                        D+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMETWFGDVSLNTIFRMMVGKRFSTDSEGSGGEKYRQALRDVFELFGAFVPSDSF 240

BLAST of Lcy04g002740 vs. NCBI nr
Match: XP_023535447.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.7 bits (818), Expect = 2.0e-83
Identity = 176/294 (59.86%), Postives = 188/294 (63.95%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCG---KKRLPPEAGGAWPVIGHLHKLSAREP 60
           MEL + L  +FALLLF YALF I+ R S       KRLPPEA GA P+IGHLHKLSA EP
Sbjct: 1   MELTRTLPTTFALLLFLYALFRIASRRSSAAHRTNKRLPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYN 120
           TH+TLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASRPK LASELLGYN
Sbjct: 61  THLTLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRPKGLASELLGYN 120

Query: 121 YASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE-------------------- 180
           Y  + +SPY PYWR VRKI TLEL TNHRL QLQHIRI E                    
Sbjct: 121 YTMLSISPYGPYWRRVRKIVTLELLTNHRLHQLQHIRICEVQNSMKKLYELNKGSGGKVR 180

Query: 181 -----------------------------------------DLLVLFGAFVPSDSFPFLD 229
                                                    D+  LFGAFVPSDSFP L 
Sbjct: 181 VEMGTWFGDVSLNTILRMVVGKRLSSVLEGSGAEQYKEAFRDVFELFGAFVPSDSFPCLG 240

BLAST of Lcy04g002740 vs. NCBI nr
Match: XP_023535445.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318.9 bits (816), Expect = 3.5e-83
Identity = 176/293 (60.07%), Postives = 189/293 (64.51%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRCGK--KRLPPEAGGAWPVIGHLHKLSAREPT 60
           MEL  ++ A+FALLLF YAL  I+ R S   +  KRLPPEA G  PVIGHLHKLSA EPT
Sbjct: 40  MELTHIIPATFALLLFLYALLRITSRRSAAHRTNKRLPPEAAGRLPVIGHLHKLSATEPT 99

Query: 61  HITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNY 120
           HITLAKM D YGPIFTLKLG NT L+VSSWEIA+ECFTTND  FASR K LASELLGYNY
Sbjct: 100 HITLAKMADAYGPIFTLKLGMNTVLVVSSWEIARECFTTNDTIFASRSKGLASELLGYNY 159

Query: 121 ASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE--------------------- 180
             + V+PY PYWRHVRKIAT EL  N RLEQLQHIR+SE                     
Sbjct: 160 TLLSVTPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKKLYELNKGSGGKVRV 219

Query: 181 ----------------------------------------DLLVLFGAFVPSDSFPFLDW 229
                                                   D+  LFGAFVPSDSFPFL W
Sbjct: 220 EMGTWFGDVSLNTILRMVVGKRLSSVLEGSGAEQYKEASRDVFELFGAFVPSDSFPFLSW 279

BLAST of Lcy04g002740 vs. NCBI nr
Match: XP_022936281.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 318.9 bits (816), Expect = 3.5e-83
Identity = 175/299 (58.53%), Postives = 191/299 (63.88%), Query Frame = 0

Query: 1   MELAQVLAASFALLLFFYALFSISRRFSRC---GKKRLPPEAGGAWPVIGHLHKLSAREP 60
           MEL  +L A+FALLLF YALF I+ R S       KR PPEA GA P+IGHLHKLSA EP
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THITLAKMGDVYGPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYN 120
           THITLAKM D YGPIFTL+LG NTAL+VSSWEIA+ECFTTND  FASR K++ASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YASMGVSPYDPYWRHVRKIATLELRTNHRLEQLQHIRISE-------------------- 180
           YA++G+SPY PYWRHVRK+ATLEL TNHRLEQLQHIRI E                    
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 ----------------------------------------------DLLVLFGAFVPSDS 229
                                                             LF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

BLAST of Lcy04g002740 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 181.4 bits (459), Expect = 8.1e-46
Identity = 107/286 (37.41%), Postives = 149/286 (52.10%), Query Frame = 0

Query: 13  LLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREP-THITLAKMGDVYGP 72
           L+  F ALF  S++     K    P   GAWP+IGHLH L  +E   + TL KM D YGP
Sbjct: 13  LVFVFIALFKKSKK----PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGP 72

Query: 73  IFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWR 132
             +L+LG+N A +VSS+E+AK+CFT ND+  ASRP   A++ +GYN+A  G +PY  +WR
Sbjct: 73  AMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWR 132

Query: 133 HVRKIATLELRTNHRLEQLQHIRISE---------------------------------- 192
            +RKIAT+EL +N RL+ L+H+R+SE                                  
Sbjct: 133 EMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTL 192

Query: 193 DLLV-----------------------------------LFGAFVPSDSFPFLDWFDLGG 229
           +++V                                   L G F  SD+FP L +FDL G
Sbjct: 193 NMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQG 252

BLAST of Lcy04g002740 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 180.3 bits (456), Expect = 1.8e-45
Identity = 106/285 (37.19%), Postives = 146/285 (51.23%), Query Frame = 0

Query: 13  LLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREP-THITLAKMGDVYGP 72
           L+  F ALF  S++     K    P   GAWP+IGHLH LS +E   + TL KM D YGP
Sbjct: 13  LVFVFIALFKKSKK----PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGP 72

Query: 73  IFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWR 132
             +L+LG++   +VSS+E+AK+CFT ND+  ASRP   A++ +GY+ A  G +PY  +WR
Sbjct: 73  AMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWR 132

Query: 133 HVRKIATLELRTNHRLEQLQHIRISEDLLV------------------------------ 192
            +RKIATLEL +N RL+ L+H+R+SE  +V                              
Sbjct: 133 EMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSL 192

Query: 193 --------------------------------------LFGAFVPSDSFPFLDWFDLGGY 229
                                                 L G F  SD+FP L WFD  G+
Sbjct: 193 NMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGH 252

BLAST of Lcy04g002740 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 94/286 (32.87%), Postives = 134/286 (46.85%), Query Frame = 0

Query: 13  LLLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREP-THITLAKMGDVYGP 72
           L+  F ALF  S++     K    P   GAWP+IGHLH L  +E   + TL KM D YGP
Sbjct: 13  LVFVFIALFKKSKK----PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGP 72

Query: 73  IFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWR 132
             +L+LG++   + SS+E+AK+CFT ND+  AS     A++ +GY            +W 
Sbjct: 73  AMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY-----------VFWL 132

Query: 133 HVRKIATLELRTNHRLEQLQHIRIS------------------------------EDLLV 192
            +RKIA +EL +N RL+ L ++R+S                              ED++ 
Sbjct: 133 EMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIA 192

Query: 193 ---------------------------------------LFGAFVPSDSFPFLDWFDLGG 229
                                                  L G F  SD+FP L W DL G
Sbjct: 193 NMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQG 252

BLAST of Lcy04g002740 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 135.6 bits (340), Expect = 5.1e-32
Identity = 89/284 (31.34%), Postives = 124/284 (43.66%), Query Frame = 0

Query: 14  LLFFYALFSISRRFSRCGKKRLPPEAGGAWPVIGHLHKLSAREPTHITLAKMGDVYGPIF 73
           L  F  L  I  R     KK   P   GAWP++GHLH      PTH+T   M DVYGP+F
Sbjct: 12  LFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVF 71

Query: 74  TLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPYWRHV 133
             KLG+   +I++S E+AKE +T +D+    RP++ AS+LLGYN + +  SPY  YWR +
Sbjct: 72  MAKLGSIKVMIINSKEVAKEIYTVHDK-LLERPELTASKLLGYNDSFLTFSPYGLYWREI 131

Query: 134 RKIATLELRTNHRLEQLQHIRISE------------------------------------ 193
           RKIA  EL +   ++     R  E                                    
Sbjct: 132 RKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTAN 191

Query: 194 -------------------------------DLLVLFGAFVPSDSFP---FLDWFDLGGY 228
                                          + L  F  F+ SD  P   FLDW      
Sbjct: 192 ISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLDW----KT 251

BLAST of Lcy04g002740 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-28
Identity = 86/276 (31.16%), Postives = 123/276 (44.57%), Query Frame = 0

Query: 11  FALLLFFYALFSISRRFSRCG-KKRLPPEAGGAWPVIGHLHKLSAREPTHITLAKMGDVY 70
           F+L L  +    + ++ SRC       PE  GA P+ GHLH L  ++     LA M   +
Sbjct: 13  FSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKH 72

Query: 71  GPIFTLKLGTNTALIVSSWEIAKECFTTNDRTFASRPKMLASELLGYNYASMGVSPYDPY 130
           GPIF+LKLG    ++ S  +  K+CFTTND   A+RP +     +GYN AS+ ++PY  Y
Sbjct: 73  GPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDY 132

Query: 131 WRHVRKIATLELRTNHRLEQLQHIRISE--------------------DLLVLFGAF--- 190
           WR +RKI T+ L +NH +E L HIR SE                    D+L  F  F   
Sbjct: 133 WRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYKGNGGTSIVKIDMLFEFLTFNII 192

Query: 191 ---------------------------------VP--SDSFPFLDWFDLGGYKKAMKKTA 228
                                            +P   D  P+L W D       MK+  
Sbjct: 193 LRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDF-AKNSQMKRLF 252