Lcy03g020280 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g020280
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionExocyst subunit Exo70 family protein
LocationChr03: 53660916 .. 53663707 (-)
RNA-Seq ExpressionLcy03g020280
SyntenyLcy03g020280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGAAAGTAGCGGAGATAAAAGAATGATGGTGGAAACAGTGATAGGTAAGGAATTGAGAAAGAGAAGGTGCAGGGTTTTTACTTGGAAAGCAACGCAAGCAGCGAAACCCGAAAGGAAGATGCTTTGTCTCACTCCGCAGTTTAGTATTTATCACTCCCCTAACTCCCTTTTCCCAAATTCCAACCCGGATCCTATTCCCAACCCAAACCCGCCAAAACTCCCACTTCTCCATTTCCGCCATTAAAGCCTTCCTTCCTTTTCTTCGCTTCTCTCTTCTTCGCCCTCCAGACCCCTCAATTCAATTCCCAACTCTCGCAATGTGTTTTCCTCTTCTTCCCCATTTCCTTTTCTGATTCTGAACCAATCCGCCCAAATTTGGGGCTCGGGGATGCTTTCTGATTTTCCCATACTGGAGCTTCGGGACGCCATGCCTTTTTGCTACTGAAACGTGAAACCCCCTTGTTGTTCTTCTCCAGCTACACCCCCCCAGAAATGGCCGCCACGAATACGAATGTCGCCAGCGGCGTCGACGACCGCGTCCTGGCCACTGCTCAGCAGATCGTCAAGAGCCTCAATACCCCTAAGGAGGTTCGTGAGGACATGCTCTTGATTCTCTCCAGTTTTGATAATCGCCTTTCCAGTATCGGTAGTATGATCGGCAGTGACGACTCCAAGACCAAGAATGATACGTTGGACGCCGCGGAGAAGGTGATTCTTCGTTGGGACCCTAATTCCGATGTGTCCAGACGGTCGCTTAATTGGGAGGACTCGCCGGACGAGGCGGCTGAGTATTTGTCGGCGGTGGATGATATTCTCCGGCTACTGGAAGACCTTTCCATTGGGTCCGACTCGACTGAGATCGTAGACCGGGCCGAAAACGCGATCCAAATGGCGATGTGTCGGCTCGAGAGTGAATTCCGCCATATCCTGATCCAGAGCACAGTCCCTCTCGACGCCGAGCGGCTCTACGGCTCGATCCGAAGAGTTCACCTCTCGTTTGCGTCCAATTACAGTGAAATTGACGATGAATTCGAGAGTTTCGGCGAGGAAAGTCGCAGTTCCGGGCGATTCCATGAGCGAGGTGCGACAATTGGAGAAGATTCTTGGGTCGATTTGATCCATCCCGATGCGGCTGTTGATCTGTCGGAGATTGCGGACCGTATGATTCGTGCCGGCTACGAAAAGGAGTGTGTTCAAGTCTATAGCACCGTTCGTCGTGATGCTTTGGACGAGTGCTTGGCGATTCTTGGCGTCGAACGGCTTAGCATTGAGGAGGTTCAGAAAAGTGACTGGAAATTTTTGGATGAGAAGATGAAGAAATGGATTCAGGCCGTCAAAATTACAGTCCGGTTGATTTTGGACGGTGAGAAGAGGCTATGTGATCAGATTTTCAATGGCGCCAATGAGTCCAAGGAGATTTGCTTTAACGAAACGGCAAAGGGGTGTGTAATGCAGCTCTTGAATTTTGGTGAGGCAGTGGCTATTGGCAAGAGGTCACCGGAGAAGCTGTTCCGTATCTTGGACATGTACGATGCTTTGGCCGGCGTTTTGCCTGACTTGGAAGCCATGGTTTCCGATGAGTTTCTGATCAGTGAAGCGCATGGAGTGTTGTCTGGACTGGGAGAAGCCGCCACGGGGACATTTGCAGAGTTTGAGAATGCTATTGAAAGCGAAAATTCGAAGAAAGCAATGCAAAATGCAGAGATTCACCCACTTATTCGATATGTAATGAATTATGTGAGATTGCTGGTTGATTACAGTAAGACGATGAACTCTCTTTTAGAAGATGAGGAAGTACAAGACTTGCGGAATGTGAACGATAATGACGATGATCTGCAGTTGAAAAGCACGTCGCCTTTAGCTCGTAGATTGTTAATGCTGTTATCATCTTTGGAATCCAATCTTGTGGATAAATCAAAACTTTATGAGGATATTGCTATGCAGTTCATATTTCTAATGAACAATATACTGTATATAGTGAAAAAGGTGAAAGATTCTGAGCTTGCTCAGCTGCTTGGCGATAACTGGCTTCGCAGGCATAGCGGCCAAATTCGCCAATATGAAACAAGTTACCTTAGAGCCTCTTGGAGCAAAGTTTTGTCTTGCTTAAAGGATGAAGGTATTGGTGGGAGCACTAGCAATGCCTCCAAGGTAGCTTTGAAGGAAAAGTTCAAGAATTTCAATGCAGGTTTTGAAGAAATCTGCAGGGTTCAGACATCTTGGAAGGTATCGGATGCTCAACTGCGCGATGAGCTGATAATATCTGTTTCGGAGAAGGTGATTCCAGCGTATCGCTCCTTCTTGGGAAGGTTTAGAAATCAGTTAGAGAGCGGAAGGCATTCTGGGAAGTACATAAAGTACACACCAGAGGACTTAGAGAACTCTCTATCAGATTTGTTTGAAGGATCGCCTGCTGTTTCACATCACATGCGAAGAAAGGGTACTTGAATGAAATCCAGACCTGAAACTGTTTGATGGTGAGCTGTTGATCTTTTTTTTTCTTTTTTGCTAATGTTATAGACACATGATATTGTGAAGGTTATGATCTTACTTACTTTTGTTCATACACTGTATAATACATTACCAACGTTGTATACATTAGATTGTGTATCTTAATTTGAGGTCAAGTAATATTGTGGACATAACTTCAACCCCCATCCCCTGCCCCGGACCCCGACCCCCACCCCGACTAGCCAAAGTCTATTAGGCTATTACAGAGAAGAAGCAAGAGTCAAATCTGTAAAGTTGTTAGCTGCAAAATAATGCAATACTTTTGTTGTCATTTTTCTA

mRNA sequence

ATAGAAAGTAGCGGAGATAAAAGAATGATGGTGGAAACAGTGATAGGTAAGGAATTGAGAAAGAGAAGGTGCAGGGTTTTTACTTGGAAAGCAACGCAAGCAGCGAAACCCGAAAGGAAGATGCTTTGTCTCACTCCGCAGTTTAGTATTTATCACTCCCCTAACTCCCTTTTCCCAAATTCCAACCCGGATCCTATTCCCAACCCAAACCCGCCAAAACTCCCACTTCTCCATTTCCGCCATTAAAGCCTTCCTTCCTTTTCTTCGCTTCTCTCTTCTTCGCCCTCCAGACCCCTCAATTCAATTCCCAACTCTCGCAATGTGTTTTCCTCTTCTTCCCCATTTCCTTTTCTGATTCTGAACCAATCCGCCCAAATTTGGGGCTCGGGGATGCTTTCTGATTTTCCCATACTGGAGCTTCGGGACGCCATGCCTTTTTGCTACTGAAACGTGAAACCCCCTTGTTGTTCTTCTCCAGCTACACCCCCCCAGAAATGGCCGCCACGAATACGAATGTCGCCAGCGGCGTCGACGACCGCGTCCTGGCCACTGCTCAGCAGATCGTCAAGAGCCTCAATACCCCTAAGGAGGTTCGTGAGGACATGCTCTTGATTCTCTCCAGTTTTGATAATCGCCTTTCCAGTATCGGTAGTATGATCGGCAGTGACGACTCCAAGACCAAGAATGATACGTTGGACGCCGCGGAGAAGGTGATTCTTCGTTGGGACCCTAATTCCGATGTGTCCAGACGGTCGCTTAATTGGGAGGACTCGCCGGACGAGGCGGCTGAGTATTTGTCGGCGGTGGATGATATTCTCCGGCTACTGGAAGACCTTTCCATTGGGTCCGACTCGACTGAGATCGTAGACCGGGCCGAAAACGCGATCCAAATGGCGATGTGTCGGCTCGAGAGTGAATTCCGCCATATCCTGATCCAGAGCACAGTCCCTCTCGACGCCGAGCGGCTCTACGGCTCGATCCGAAGAGTTCACCTCTCGTTTGCGTCCAATTACAGTGAAATTGACGATGAATTCGAGAGTTTCGGCGAGGAAAGTCGCAGTTCCGGGCGATTCCATGAGCGAGGTGCGACAATTGGAGAAGATTCTTGGGTCGATTTGATCCATCCCGATGCGGCTGTTGATCTGTCGGAGATTGCGGACCGTATGATTCGTGCCGGCTACGAAAAGGAGTGTGTTCAAGTCTATAGCACCGTTCGTCGTGATGCTTTGGACGAGTGCTTGGCGATTCTTGGCGTCGAACGGCTTAGCATTGAGGAGGTTCAGAAAAGTGACTGGAAATTTTTGGATGAGAAGATGAAGAAATGGATTCAGGCCGTCAAAATTACAGTCCGGTTGATTTTGGACGGTGAGAAGAGGCTATGTGATCAGATTTTCAATGGCGCCAATGAGTCCAAGGAGATTTGCTTTAACGAAACGGCAAAGGGGTGTGTAATGCAGCTCTTGAATTTTGGTGAGGCAGTGGCTATTGGCAAGAGGTCACCGGAGAAGCTGTTCCGTATCTTGGACATGTACGATGCTTTGGCCGGCGTTTTGCCTGACTTGGAAGCCATGGTTTCCGATGAGTTTCTGATCAGTGAAGCGCATGGAGTGTTGTCTGGACTGGGAGAAGCCGCCACGGGGACATTTGCAGAGTTTGAGAATGCTATTGAAAGCGAAAATTCGAAGAAAGCAATGCAAAATGCAGAGATTCACCCACTTATTCGATATGTAATGAATTATGTGAGATTGCTGGTTGATTACAGTAAGACGATGAACTCTCTTTTAGAAGATGAGGAAGTACAAGACTTGCGGAATGTGAACGATAATGACGATGATCTGCAGTTGAAAAGCACGTCGCCTTTAGCTCGTAGATTGTTAATGCTGTTATCATCTTTGGAATCCAATCTTGTGGATAAATCAAAACTTTATGAGGATATTGCTATGCAGTTCATATTTCTAATGAACAATATACTGTATATAGTGAAAAAGGTGAAAGATTCTGAGCTTGCTCAGCTGCTTGGCGATAACTGGCTTCGCAGGCATAGCGGCCAAATTCGCCAATATGAAACAAGTTACCTTAGAGCCTCTTGGAGCAAAGTTTTGTCTTGCTTAAAGGATGAAGGTATTGGTGGGAGCACTAGCAATGCCTCCAAGGTAGCTTTGAAGGAAAAGTTCAAGAATTTCAATGCAGGTTTTGAAGAAATCTGCAGGGTTCAGACATCTTGGAAGGTATCGGATGCTCAACTGCGCGATGAGCTGATAATATCTGTTTCGGAGAAGGTGATTCCAGCGTATCGCTCCTTCTTGGGAAGGTTTAGAAATCAGTTAGAGAGCGGAAGGCATTCTGGGAAGTACATAAAGTACACACCAGAGGACTTAGAGAACTCTCTATCAGATTTGTTTGAAGGATCGCCTGCTGTTTCACATCACATGCGAAGAAAGGGTACTTGAATGAAATCCAGACCTGAAACTGTTTGATGGTGAGCTGTTGATCTTTTTTTTTCTTTTTTGCTAATGTTATAGACACATGATATTGTGAAGGTTATGATCTTACTTACTTTTGTTCATACACTGTATAATACATTACCAACGTTGTATACATTAGATTGTGTATCTTAATTTGAGGTCAAGTAATATTGTGGACATAACTTCAACCCCCATCCCCTGCCCCGGACCCCGACCCCCACCCCGACTAGCCAAAGTCTATTAGGCTATTACAGAGAAGAAGCAAGAGTCAAATCTGTAAAGTTGTTAGCTGCAAAATAATGCAATACTTTTGTTGTCATTTTTCTA

Coding sequence (CDS)

ATGGCCGCCACGAATACGAATGTCGCCAGCGGCGTCGACGACCGCGTCCTGGCCACTGCTCAGCAGATCGTCAAGAGCCTCAATACCCCTAAGGAGGTTCGTGAGGACATGCTCTTGATTCTCTCCAGTTTTGATAATCGCCTTTCCAGTATCGGTAGTATGATCGGCAGTGACGACTCCAAGACCAAGAATGATACGTTGGACGCCGCGGAGAAGGTGATTCTTCGTTGGGACCCTAATTCCGATGTGTCCAGACGGTCGCTTAATTGGGAGGACTCGCCGGACGAGGCGGCTGAGTATTTGTCGGCGGTGGATGATATTCTCCGGCTACTGGAAGACCTTTCCATTGGGTCCGACTCGACTGAGATCGTAGACCGGGCCGAAAACGCGATCCAAATGGCGATGTGTCGGCTCGAGAGTGAATTCCGCCATATCCTGATCCAGAGCACAGTCCCTCTCGACGCCGAGCGGCTCTACGGCTCGATCCGAAGAGTTCACCTCTCGTTTGCGTCCAATTACAGTGAAATTGACGATGAATTCGAGAGTTTCGGCGAGGAAAGTCGCAGTTCCGGGCGATTCCATGAGCGAGGTGCGACAATTGGAGAAGATTCTTGGGTCGATTTGATCCATCCCGATGCGGCTGTTGATCTGTCGGAGATTGCGGACCGTATGATTCGTGCCGGCTACGAAAAGGAGTGTGTTCAAGTCTATAGCACCGTTCGTCGTGATGCTTTGGACGAGTGCTTGGCGATTCTTGGCGTCGAACGGCTTAGCATTGAGGAGGTTCAGAAAAGTGACTGGAAATTTTTGGATGAGAAGATGAAGAAATGGATTCAGGCCGTCAAAATTACAGTCCGGTTGATTTTGGACGGTGAGAAGAGGCTATGTGATCAGATTTTCAATGGCGCCAATGAGTCCAAGGAGATTTGCTTTAACGAAACGGCAAAGGGGTGTGTAATGCAGCTCTTGAATTTTGGTGAGGCAGTGGCTATTGGCAAGAGGTCACCGGAGAAGCTGTTCCGTATCTTGGACATGTACGATGCTTTGGCCGGCGTTTTGCCTGACTTGGAAGCCATGGTTTCCGATGAGTTTCTGATCAGTGAAGCGCATGGAGTGTTGTCTGGACTGGGAGAAGCCGCCACGGGGACATTTGCAGAGTTTGAGAATGCTATTGAAAGCGAAAATTCGAAGAAAGCAATGCAAAATGCAGAGATTCACCCACTTATTCGATATGTAATGAATTATGTGAGATTGCTGGTTGATTACAGTAAGACGATGAACTCTCTTTTAGAAGATGAGGAAGTACAAGACTTGCGGAATGTGAACGATAATGACGATGATCTGCAGTTGAAAAGCACGTCGCCTTTAGCTCGTAGATTGTTAATGCTGTTATCATCTTTGGAATCCAATCTTGTGGATAAATCAAAACTTTATGAGGATATTGCTATGCAGTTCATATTTCTAATGAACAATATACTGTATATAGTGAAAAAGGTGAAAGATTCTGAGCTTGCTCAGCTGCTTGGCGATAACTGGCTTCGCAGGCATAGCGGCCAAATTCGCCAATATGAAACAAGTTACCTTAGAGCCTCTTGGAGCAAAGTTTTGTCTTGCTTAAAGGATGAAGGTATTGGTGGGAGCACTAGCAATGCCTCCAAGGTAGCTTTGAAGGAAAAGTTCAAGAATTTCAATGCAGGTTTTGAAGAAATCTGCAGGGTTCAGACATCTTGGAAGGTATCGGATGCTCAACTGCGCGATGAGCTGATAATATCTGTTTCGGAGAAGGTGATTCCAGCGTATCGCTCCTTCTTGGGAAGGTTTAGAAATCAGTTAGAGAGCGGAAGGCATTCTGGGAAGTACATAAAGTACACACCAGAGGACTTAGAGAACTCTCTATCAGATTTGTTTGAAGGATCGCCTGCTGTTTCACATCACATGCGAAGAAAGGGTACTTGA

Protein sequence

MAATNTNVASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDDSKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDSTEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDEFESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQIFNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAMVSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLKDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT
Homology
BLAST of Lcy03g020280 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.7e-107
Identity = 226/643 (35.15%), Postives = 377/643 (58.63%), Query Frame = 0

Query: 8   VASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSS---IGSMIGSDDS--KT 67
           +A   ++++LA A+ I K+L   + + +D+L I S+FD R S         G D S   T
Sbjct: 1   MAENGEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRFSREKLAEGQAGEDGSGVAT 60

Query: 68  KNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDSTE 127
               L++ +  I R+     V+     W D  D AA +L  +D+++ ++ + S  +    
Sbjct: 61  LERALNSIDGQISRF-----VAADQPIWADPADSAA-FLDTIDELVAIIREWSPMASEKP 120

Query: 128 I---VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 187
           I   + RA++ +Q AM R+E EFR ++ +           G    ++  F S   E DD 
Sbjct: 121 IGICLTRADDMMQQAMFRIEEEFRSLMERGAESFGLNP-QGDAGAMNHRFDSEEEEDDDR 180

Query: 188 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 247
             + G++     +          D  +D +      DL E+A RM+ AG+ K C  VYS+
Sbjct: 181 DFNNGDDI----QIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSS 240

Query: 248 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 307
            RR+ L+E ++ LG+++LSIEEV K  W+ L++++ +WI+A  + +R++   E+RLCD++
Sbjct: 241 CRREFLEESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRV 300

Query: 308 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 367
           F G + + ++ F E  +G  +QLLNF +A+AIG RSPE+LF++LD+++ +  ++P+ E++
Sbjct: 301 FFGFSSAADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESV 360

Query: 368 VSDEF---LISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYV 427
            SD+F   L +EA  +   LGEA  G F E EN I  + +K A+    +HP+ RYVMNY+
Sbjct: 361 FSDQFCSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYL 420

Query: 428 RLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSK 487
           R      +T+  + E+             + +  K ++ L  ++  ++  LESNL  KSK
Sbjct: 421 RAACRSRQTLEQVFEE------------SNGVPSKDSTLLTVQMSWIMELLESNLEVKSK 480

Query: 488 LYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVL 547
           +Y+D A+ ++FLMNN  YIV+KVKD +L  LLGD+W+R+H+ +++QY  +Y R+SW+K+L
Sbjct: 481 VYKDPALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKML 540

Query: 548 SCLKDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKV 607
             LK +      +   K  +KEK K FN  F+EIC+V ++W V D QL++EL IS++  +
Sbjct: 541 GLLKVDNTAAGMNGLGK-TMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLL 600

Query: 608 IPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGS 640
           +PAY SF+GRF+N  + G+++ KYIKY  ED+E  +++LF+G+
Sbjct: 601 VPAYGSFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKGT 619

BLAST of Lcy03g020280 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 6.9e-95
Identity = 221/655 (33.74%), Postives = 357/655 (54.50%), Query Frame = 0

Query: 12  VDDRVLATAQQIV---KSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDDSKTK----- 71
           VD R+   +++ V    SL   + + ++++ IL SFD+RLS++ + +     +T      
Sbjct: 3   VDSRMDLLSERAVLMRASLQKSQTITDNVVSILGSFDSRLSALETAMRPTQIRTHAIRKA 62

Query: 72  ----NDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAE-YLSAVDD---ILRLLEDLS 131
               + TL AAE ++ ++D       + L     P E  E YL A+     I+R      
Sbjct: 63  HENIDRTLKAAEVILSQFDLLRQAETKVLK---GPHEDLESYLDAIAQLRKIIRYFMSNK 122

Query: 132 IGSDSTEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSE 191
               S  +++ A + +  A  +LE EF+ +L   +  ++ +RL+  +         N   
Sbjct: 123 SFKSSDGVLNHANSLLAKAQSKLEEEFKQLLASYSKAVEPDRLFDGL--------PNSLR 182

Query: 192 IDDEFESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQ 251
              + +  G   +  G  H   A     +   LI       L ++A +M++AG++++ +Q
Sbjct: 183 PSSDGDGGG---KPHGGHHNDDAETAAYTLPILIPSRVLPLLHDLAQQMVQAGHQQQLLQ 242

Query: 252 VYSTVRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRL 311
           +Y   R   L+E L  LGVE+LS E+VQ+  W+ L+ K+  WI  ++I V+L+  GE+++
Sbjct: 243 IYRDTRSFVLEESLKKLGVEKLSKEDVQRMQWEVLEAKIGNWIHFMRIAVKLLFAGERQV 302

Query: 312 CDQIFNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPD 371
           CDQIF G +   + CF E     V  LL+FG+A+A  KRSPEKLF +LDMY+ +  +  +
Sbjct: 303 CDQIFRGFDSLSDQCFAEVTVSSVSMLLSFGDAIARSKRSPEKLFVLLDMYEIMRELHTE 362

Query: 372 LEAMVSDEFLI---SEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYV 431
           +E +   +  +     A G+   L + A  TF +FE A+E + +K A+ +  +HPL  YV
Sbjct: 363 IETIFKGKACLEIRDSATGLTKRLAQTAQETFGDFEEAVEKDATKTAVLDGTVHPLTSYV 422

Query: 432 MNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLV 491
           +NYV+ L DY  T+  L  +           N DD    S S LA   + ++ +L++NL 
Sbjct: 423 INYVKFLFDYQTTLKQLFLE---------FGNGDD----SNSQLASVTMRIMQALQNNLD 482

Query: 492 DKSKLYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASW 551
            KSK Y+D A+  +FLMNNI Y+V+ V+ SE   LLGD+W++RH   ++Q+   Y R +W
Sbjct: 483 GKSKQYKDPALTHLFLMNNIHYMVRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAW 542

Query: 552 SKVLSCLKDEGI---------GGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQ 611
           +K+L     +G+         GG++S  S+  LKE+FK FN  F+E+ + Q+ W V D +
Sbjct: 543 TKILQSSSAQGLTSSGGGSLEGGNSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTE 602

Query: 612 LRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEG 639
           LR+ L ++V+E ++PAYRSFL RF   +ESG++  KYIKYT EDLE  L +LFEG
Sbjct: 603 LRESLRLAVAEVLLPAYRSFLKRFGPLVESGKNPQKYIKYTAEDLERLLGELFEG 630

BLAST of Lcy03g020280 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.7e-88
Identity = 212/641 (33.07%), Postives = 342/641 (53.35%), Query Frame = 0

Query: 8   VASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDDSKTKNDTL 67
           +A   D+ + A A+ I ++L        D+L ILS +    +  G    +      N   
Sbjct: 1   MAEAGDENLYAAARDIARALGKDPSAAGDILQILSGYGASGNRGGDPRPTPSRGGSNVNF 60

Query: 68  DAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDL-SIGSDSTEIV-- 127
           D A   + R   +  V  R + W D P ++  +L +VD++L +  DL S+  D +  V  
Sbjct: 61  DRALTSLERQISSYIVEDRPI-WSD-PVDSRTFLDSVDELLAIAGDLRSMAGDKSVAVCQ 120

Query: 128 DRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDEFESFG 187
            RA+  IQ  M RL+ EF  ++ ++    D+                     DDEF    
Sbjct: 121 SRADELIQQVMFRLQEEFGFVMDRAPDSFDS---------------------DDEFPGEE 180

Query: 188 EESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYSTVRRDA 247
           +   S G    R  T      ++ +      DL+ IA RM+  G+ KEC +VYS+ RR+ 
Sbjct: 181 DNDTSDGVIVARPIT-DYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSRRREF 240

Query: 248 LDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQIFNGAN 307
           L+E L+ L +  LS+EEVQ+S W+ L++++ +WI+AV +   +    E+ LCD++F+   
Sbjct: 241 LEESLSRLHLRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVFSDLP 300

Query: 308 ESK--EICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAMVSD 367
            S   ++ F E  +G   QLLNF +A+A+G R PE+LF+++D+Y+A+  ++P +E + SD
Sbjct: 301 VSSVTDLSFMEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMETLFSD 360

Query: 368 EF---LISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 427
            +   L  EA  +   LGEA  G F E EN I  +  K A     IHP+ RYVMNY+R  
Sbjct: 361 RYCSPLRHEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNYLRAA 420

Query: 428 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 487
               +++  +L+       +  N+   D     T PL+ +++ +L  LESNL  K + Y 
Sbjct: 421 CKSRQSLEQILD-------QTGNETGSD-----TRPLSVQIIWVLELLESNLEGKKRTYR 480

Query: 488 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 547
           D ++ F+F+MNN  YI+ K KD+EL  +LG++W+ +H+ ++RQY ++Y R+SW++V+  L
Sbjct: 481 DPSLCFLFMMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRSSWNQVVGLL 540

Query: 548 KDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPA 607
           + +G            L E  + F + F+E+C+VQ+ W VSD QLR+EL  SV+  V PA
Sbjct: 541 RTDG--------PYPKLVENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVAGIVSPA 597

Query: 608 YRSFLGRFRNQLE-SGRHSGKYIKYTPEDLENSLSDLFEGS 640
           Y +F+ R +   E +GR    +I YT ED+E  +  LF+ S
Sbjct: 601 YSNFIRRLKESPEINGRRGEPFIPYTVEDVEFIIKRLFKES 597

BLAST of Lcy03g020280 vs. ExPASy Swiss-Prot
Match: Q9FNR3 (Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 4.8e-88
Identity = 218/645 (33.80%), Postives = 347/645 (53.80%), Query Frame = 0

Query: 10  SGVDDRVLATAQQIVKSLN-TPKEVREDMLLILSSFDNRLSSIG----SMIGSDDSKTKN 69
           SG+++ V      +VK+L  +   +  ++  +LS  +  LS+ G     +  +  S+ K 
Sbjct: 11  SGMNNHVFEACHHVVKALRASDNNLDANLRKLLSDLEMHLSTFGIADTKVEDAGFSEIKK 70

Query: 70  DTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLE-----DLSIGSD 129
              +A ++ I  W+ N     +S  +E    EA ++  A+ D+  +L       +     
Sbjct: 71  RFKEAVKR-IRSWETN-----QSTMFEAGLSEADQFFQALYDVQTVLVGFKALPMKTNQM 130

Query: 130 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 189
             ++ ++A  A+ +AM RLE E   +L Q    +  + L  S RR  + +       D+ 
Sbjct: 131 EKDVYNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVY-------DES 190

Query: 190 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 249
           F S  +E       HE    I +    DL+ P     +  IA+ M    Y++   + +  
Sbjct: 191 FVSLDDEVIVEASSHEDDEQISDFYNSDLVDPIVLPHIKAIANAMFACEYDQPFCEAFIG 250

Query: 250 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 309
           V+R+AL+E +  L +ER S  +V + DW+ L+  M+KW + VKI  ++ L  EK+LCDQI
Sbjct: 251 VQREALEEYMVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQI 310

Query: 310 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 369
                     CF E +K  ++ LLNFGEAV +    PE L R L MY+  A +L D++ +
Sbjct: 311 LGDFESISTACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNL 370

Query: 370 VSDE---FLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYV 429
             DE    L    H +   L +  T TF +F++AI S+ S +      IH L RYVMNY+
Sbjct: 371 FPDETGSSLRIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYL 430

Query: 430 RLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSK 489
           +LL +Y+ ++NSLL++  V D       +D L   + SP+AR L  ++++LES+L  K++
Sbjct: 431 KLLPEYTDSLNSLLQNIHVDDSIPEKTGEDVLP-STFSPMARHLRSIVTTLESSLERKAQ 490

Query: 490 LYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVL 549
           LY D A++ IFLMNN  Y+V+KVK SEL +L GD W+R+H    +   T+Y R++WS +L
Sbjct: 491 LYADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERSTWSSIL 550

Query: 550 SCLKDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKV 609
           + L+D       +N S   L+E+ + F+  F+++ + QT W V D++LRD+L IS S KV
Sbjct: 551 ALLRD-------NNDSVRTLRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSVKV 610

Query: 610 IPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPA 642
           + +YR FLG  RN +  G    K+I+YT ED+EN L DLFE  P+
Sbjct: 611 VQSYRGFLG--RNAVRIGE---KHIRYTCEDIENMLLDLFECLPS 629

BLAST of Lcy03g020280 vs. ExPASy Swiss-Prot
Match: F4KG58 (Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 6.1e-75
Identity = 165/486 (33.95%), Postives = 270/486 (55.56%), Query Frame = 0

Query: 172 NYSEIDDEFESF----------GEESRSSGRFHE-RGATIGEDSWV--DLIHPDAAVDLS 231
           NYS++ +  + F           ++  S G+ H+ +   +G+  +    +I P     L 
Sbjct: 106 NYSKLTEPNQLFECLPSNLRPPSDDEGSDGKSHDPQSNGLGKTDYTVPTIIPPTVLPVLH 165

Query: 232 EIADRMIRAGYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWI 291
           ++A +M++AG+++E  + Y  +RR  L + L  LGVER S  +V++ +    + K+  WI
Sbjct: 166 DLAQQMVKAGHQQELFKTYRDIRRAVLAQSLEKLGVERHSKYDVERMNQDVFEAKIMNWI 225

Query: 292 QAVKITVRLILDGEKRLCDQIFNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEK 351
             ++I+V+L+   EK +C QI +G    ++  F E        LL+FG A+AI +RSPEK
Sbjct: 226 HYIRISVKLLFAAEKEICHQILDGVEPFRDQSFAEITTISFGMLLSFGYAIAISRRSPEK 285

Query: 352 LFRILDMYDALAGVLPDLEAMVSDE---FLISEAHGVLSGLGEAATGTFAEFENAIESEN 411
           +F ILDMY+ +  + P+ E +   +    +  +A  +   L +    T A+FE AIE + 
Sbjct: 286 VFVILDMYEIMIELQPEFELIFGSKPCTEMKEDALNLTKLLAQTVKETIADFEVAIEMDA 345

Query: 412 SKKAMQNAEIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSP 471
           ++  + +  +H L  YV  YV+ L DY  T+  L ++         N ND D +LKS   
Sbjct: 346 TETVVMDGSVHALTSYVARYVKFLFDYEPTLRQLFQE--------FNSNDPDTKLKSV-- 405

Query: 472 LARRLLMLLSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRR 531
               +  ++ +L +NL  KS+ +ED A+  +FLMNN+ YIV+  +  E    LGD+ ++ 
Sbjct: 406 ----MTGIMRALRNNLDGKSRQFEDAALTQLFLMNNVYYIVRNFRREEAKNFLGDDLVQT 465

Query: 532 HSGQIRQYETSYLRASWSKVLSCLK-DEGIGGSTSNAS--KVALKEKFKNFNAGFEEICR 591
           H   ++Q+   Y   SW+K+L C+       G   N S  K  +KEKFK FN+ FEE+ +
Sbjct: 466 HRRIVQQHAKQYQTISWNKILQCITVQSSKSGLIKNESIKKTLVKEKFKTFNSQFEELHQ 525

Query: 592 VQTSWKVSDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSL 639
            Q  W VSD +LR+ L ++++E ++PAY SFL RF   +ESG++S KYI++TPEDLE  L
Sbjct: 526 RQCQWSVSDVELRESLRLAIAEVLLPAYGSFLKRFGPMIESGKNSQKYIRFTPEDLERML 577

BLAST of Lcy03g020280 vs. ExPASy TrEMBL
Match: A0A6J1KKZ6 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495521 PE=3 SV=1)

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 614/653 (94.03%), Postives = 630/653 (96.48%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAATNT+V AS VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATNTDVAASAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKND LDAAEKVILRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDI+RL+EDLSIGS+
Sbjct: 61  SKTKNDRLDAAEKVILRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDIVRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQS VPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSAVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+FGE+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFGEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLA RLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLAHRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTP+DLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPDDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. ExPASy TrEMBL
Match: A0A6J1EKA6 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111435130 PE=3 SV=1)

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 612/653 (93.72%), Postives = 628/653 (96.17%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAAT T+V AS VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATKTDVAASAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKND LDAAEKVILRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDILRL+EDLSIGS+
Sbjct: 61  SKTKNDRLDAAEKVILRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDILRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+F E+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFSEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLD+KMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDDKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGA+ESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGAHESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           V DEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VFDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLARRLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLARRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICR QTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRAQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. ExPASy TrEMBL
Match: A0A6J1E1E8 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111429722 PE=3 SV=1)

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 605/652 (92.79%), Postives = 627/652 (96.17%), Query Frame = 0

Query: 1   MAATNTNVASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSD-D 60
           MAATNTNVA+GVDDRVLATAQQIVKSLNTPKEVREDMLLILS+FDNRLSSIGSMI SD +
Sbjct: 1   MAATNTNVATGVDDRVLATAQQIVKSLNTPKEVREDMLLILSNFDNRLSSIGSMINSDNN 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           S T+ND LDAAEKVILRWDPNSDV R SLNWEDSPDEAAEYLSAVDDI RL+EDLSIGS+
Sbjct: 61  SNTENDRLDAAEKVILRWDPNSDVPRPSLNWEDSPDEAAEYLSAVDDIRRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           ST+IV RAENAIQMAMCRLESEFRHILIQSTVPLDA+RLYGSIRRVHLSFAS+YSEIDDE
Sbjct: 121 STKIVARAENAIQMAMCRLESEFRHILIQSTVPLDADRLYGSIRRVHLSFASHYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FESFGEESR+SGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FESFGEESRNSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLIL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           F+GANES+EICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLP LEAM
Sbjct: 301 FSGANESQEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPHLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISE HGVLSGLGEAATGTF EFENAI SENSKKAMQNA+IHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEVHGVLSGLGEAATGTFVEFENAILSENSKKAMQNADIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE QD+ N  DNDD LQL+STSPLARRL MLLSSLESNLV+KSK YE
Sbjct: 421 VDYSKTMNSLLEDEEEQDMPNEKDNDDILQLESTSPLARRLFMLLSSLESNLVEKSKFYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D++MQFIFLMNNILYIVKKVKDSELAQLLGDNWLRR SGQIRQYET+YLRASWSKVLSCL
Sbjct: 481 DVSMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRRSGQIRQYETTYLRASWSKVLSCL 540

Query: 541 KDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPA 600
           KDEGIGGS SNASKVALKEKFKNFNAGFEEICRVQT+WKVSD QLRDELIISVSEKVIPA
Sbjct: 541 KDEGIGGSPSNASKVALKEKFKNFNAGFEEICRVQTAWKVSDTQLRDELIISVSEKVIPA 600

Query: 601 YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGS AVSHH+RRK T
Sbjct: 601 YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSLAVSHHLRRKST 652

BLAST of Lcy03g020280 vs. ExPASy TrEMBL
Match: A0A6J1JIF9 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111484744 PE=3 SV=1)

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 604/652 (92.64%), Postives = 624/652 (95.71%), Query Frame = 0

Query: 1   MAATNTNVASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD- 60
           MAATNTNVA+GVDDRVLATAQQIVKSLNTPKEVREDMLLILS+FDNRLSSIGSMI SD+ 
Sbjct: 1   MAATNTNVATGVDDRVLATAQQIVKSLNTPKEVREDMLLILSNFDNRLSSIGSMINSDNT 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           S  +ND LDAAEKVILRWDPNSDV R SLNWEDSPDEAAEYLSAVDDILRL+EDLSIGS+
Sbjct: 61  SNIENDRLDAAEKVILRWDPNSDVPRPSLNWEDSPDEAAEYLSAVDDILRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           S +IV RAENAIQMAMCRLESEFRHILIQSTVPLDA+RLYGSIRRVHLSFAS+YSEIDDE
Sbjct: 121 SIKIVGRAENAIQMAMCRLESEFRHILIQSTVPLDADRLYGSIRRVHLSFASHYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FESFGEESR+SGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FESFGEESRNSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLIL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGANES+EICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLP LEAM
Sbjct: 301 FNGANESQEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPHLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISE HGVLSGLGEAATGTF EFENAI SENSKKAMQNA+IHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEVHGVLSGLGEAATGTFVEFENAILSENSKKAMQNADIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE QD+ N  DNDD LQL+S SPLARRL MLLSSLESNLV+KSK YE
Sbjct: 421 VDYSKTMNSLLEDEEEQDMPNEKDNDDILQLESKSPLARRLFMLLSSLESNLVEKSKFYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D++MQFIFLMNNILYIVKKVKDSELAQLLGDNWLRR SGQIRQYETSYLRASWSKVLSCL
Sbjct: 481 DVSMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRRSGQIRQYETSYLRASWSKVLSCL 540

Query: 541 KDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPA 600
           KDEGIGGS SNASKVALKEKFKNFNA FEEICRVQT+WKVSD QLRDELIISVSEKVIPA
Sbjct: 541 KDEGIGGSPSNASKVALKEKFKNFNASFEEICRVQTAWKVSDTQLRDELIISVSEKVIPA 600

Query: 601 YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGS AVSHH+RRK T
Sbjct: 601 YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSLAVSHHLRRKST 652

BLAST of Lcy03g020280 vs. ExPASy TrEMBL
Match: A0A5D3E0I4 (Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00150 PE=3 SV=1)

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 595/652 (91.26%), Postives = 623/652 (95.55%), Query Frame = 0

Query: 1   MAATNTNVASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDDS 60
           MAATNTNV+   +DRVLATAQQIVKSLNTPKEVREDML ILS+FDNRLSSIG+MI +DDS
Sbjct: 1   MAATNTNVSGDDEDRVLATAQQIVKSLNTPKEVREDMLFILSTFDNRLSSIGTMINNDDS 60

Query: 61  KTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDS 120
             KN  LDAAEKVILRWDP+SD SRRS NWEDSP+EAAEYLSAVDDIL+LLE+LSIGS+S
Sbjct: 61  NVKNSRLDAAEKVILRWDPSSDQSRRSFNWEDSPEEAAEYLSAVDDILQLLEELSIGSES 120

Query: 121 TEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDEF 180
           T+IVDRAEN IQMAMC+LESEFRHILIQST+PLDAERLYGSIRRVHLSFAS+YSEIDDEF
Sbjct: 121 TDIVDRAENLIQMAMCQLESEFRHILIQSTIPLDAERLYGSIRRVHLSFASHYSEIDDEF 180

Query: 181 ESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYSTV 240
           ESFGEE+RSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYSTV
Sbjct: 181 ESFGEETRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYSTV 240

Query: 241 RRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQIF 300
           RRDALDECL ILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLIL+GEKRL DQIF
Sbjct: 241 RRDALDECLMILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILEGEKRLYDQIF 300

Query: 301 NGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAMV 360
           NGANESKE+CFNETAKGCVMQLL FGEAVAIGKRSPEKLFRILDMYDALAGVL DLEAMV
Sbjct: 301 NGANESKEVCFNETAKGCVMQLLTFGEAVAIGKRSPEKLFRILDMYDALAGVLRDLEAMV 360

Query: 361 SDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLLV 420
           SDEFLISEAHGVL GLGEAA GTF EFENAIESENSKKAMQNAEIHPL+RYVMNYVRLLV
Sbjct: 361 SDEFLISEAHGVLCGLGEAALGTFVEFENAIESENSKKAMQNAEIHPLVRYVMNYVRLLV 420

Query: 421 DYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKST-SPLARRLLMLLSSLESNLVDKSKLYE 480
           DYSKTMNSLLEDEEVQ   N  DN D+LQL+ST SPLARRLLMLLSSLESNL++K+KLYE
Sbjct: 421 DYSKTMNSLLEDEEVQGSPNKRDNLDNLQLESTLSPLARRLLMLLSSLESNLMEKAKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+AMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLS L
Sbjct: 481 DVAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSFL 540

Query: 541 KDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPA 600
           KDEGIGGSTSNASKVALKEKFKNFNA FEEICRVQT+WKVSDAQLRDELIISVSEKVIPA
Sbjct: 541 KDEGIGGSTSNASKVALKEKFKNFNASFEEICRVQTAWKVSDAQLRDELIISVSEKVIPA 600

Query: 601 YRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           YRSFLGRFRNQLESGRHSGKYIKYTP+DLENSLSDLFEGSP VSHH+RRKGT
Sbjct: 601 YRSFLGRFRNQLESGRHSGKYIKYTPDDLENSLSDLFEGSPVVSHHLRRKGT 652

BLAST of Lcy03g020280 vs. NCBI nr
Match: XP_023001365.1 (exocyst complex component EXO70B1-like [Cucurbita maxima])

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 614/653 (94.03%), Postives = 630/653 (96.48%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAATNT+V AS VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATNTDVAASAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKND LDAAEKVILRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDI+RL+EDLSIGS+
Sbjct: 61  SKTKNDRLDAAEKVILRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDIVRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQS VPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSAVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+FGE+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFGEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLA RLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLAHRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTP+DLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPDDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. NCBI nr
Match: XP_023529911.1 (exocyst complex component EXO70B1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 612/653 (93.72%), Postives = 629/653 (96.32%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAAT T+V AS VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATKTDVAASAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKN  LDAAEKV+LRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDILRL+EDLSIGS+
Sbjct: 61  SKTKNHRLDAAEKVLLRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDILRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+F E+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFSEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLD+KMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDDKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLARRLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLARRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTP+DLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPDDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. NCBI nr
Match: KAG6587413.1 (Exocyst complex component EXO70B1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021396.1 Exocyst complex component EXO70B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 611/653 (93.57%), Postives = 629/653 (96.32%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAAT T+V A+ VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATKTDVAANAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKND LDAAEKVILRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDILRL+EDLSIGS+
Sbjct: 61  SKTKNDRLDAAEKVILRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDILRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+F E+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFSEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLD+KMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDDKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGA+ESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGAHESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           VSDEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VSDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLARRLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLARRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICR QTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRAQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTP+DLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPDDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. NCBI nr
Match: XP_022928239.1 (exocyst complex component EXO70B1-like [Cucurbita moschata])

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 612/653 (93.72%), Postives = 628/653 (96.17%), Query Frame = 0

Query: 1   MAATNTNV-ASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDD 60
           MAAT T+V AS VDDRVL+TAQQIVKSLNTPKEVREDMLLILSSFDNRLSSI S+I SDD
Sbjct: 1   MAATKTDVAASAVDDRVLSTAQQIVKSLNTPKEVREDMLLILSSFDNRLSSISSLINSDD 60

Query: 61  SKTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSD 120
           SKTKND LDAAEKVILRWDPN DVSRRSLNWEDSPDEAAEYLSAVDDILRL+EDLSIGS+
Sbjct: 61  SKTKNDRLDAAEKVILRWDPNFDVSRRSLNWEDSPDEAAEYLSAVDDILRLVEDLSIGSE 120

Query: 121 STEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180
           STE VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE
Sbjct: 121 STEFVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 180

Query: 181 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 240
           FE+F E+SRSSGRFHERG TIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYST
Sbjct: 181 FENFSEDSRSSGRFHERGGTIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYST 240

Query: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 300
           VRRDALDECLAILGVERLSIEEVQKSDWKFLD+KMKKWIQAVK+TVR+IL GEKRLCDQI
Sbjct: 241 VRRDALDECLAILGVERLSIEEVQKSDWKFLDDKMKKWIQAVKVTVRVILVGEKRLCDQI 300

Query: 301 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360
           FNGA+ESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM
Sbjct: 301 FNGAHESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 360

Query: 361 VSDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420
           V DEFLISEAH VLSGLGEAATGTFAEFE AIESENSKKAMQNAEIHPLIRYVMNYVRLL
Sbjct: 361 VFDEFLISEAHEVLSGLGEAATGTFAEFEKAIESENSKKAMQNAEIHPLIRYVMNYVRLL 420

Query: 421 VDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYE 480
           VDYSKTMNSLLEDEE    R+VNDNDDDLQL+STSPLARRLLMLLSSLESNLVDKSKLYE
Sbjct: 421 VDYSKTMNSLLEDEE----RSVNDNDDDLQLESTSPLARRLLMLLSSLESNLVDKSKLYE 480

Query: 481 DIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCL 540
           D+ MQFIFLMNNILYIVKKVKD ELAQLLG NWLRRHSGQIRQYETSYLRASWSKVL CL
Sbjct: 481 DVGMQFIFLMNNILYIVKKVKDCELAQLLGSNWLRRHSGQIRQYETSYLRASWSKVLCCL 540

Query: 541 KDEGIGGSTSN-ASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIP 600
           KDEGIGGSTSN ASK ALKEKFKNFNAGFEEICR QTSWKVSDAQLRDELIISVSEKVIP
Sbjct: 541 KDEGIGGSTSNAASKTALKEKFKNFNAGFEEICRAQTSWKVSDAQLRDELIISVSEKVIP 600

Query: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHH+RRKGT
Sbjct: 601 AYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHLRRKGT 649

BLAST of Lcy03g020280 vs. NCBI nr
Match: XP_038877265.1 (exocyst complex component EXO70B1-like [Benincasa hispida] >XP_038877266.1 exocyst complex component EXO70B1-like [Benincasa hispida])

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 603/651 (92.63%), Postives = 628/651 (96.47%), Query Frame = 0

Query: 1   MAATNTNVASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMIGSDDS 60
           MA TNTNV  G DD VLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMI  DDS
Sbjct: 1   MATTNTNVVGGDDDHVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSSIGSMINRDDS 60

Query: 61  KTKNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDS 120
           KTKND LDAAEKVILRWDPN DVSRRS+NWED  DEAAEYLSAVDDIL+L+++LSIGS+S
Sbjct: 61  KTKNDRLDAAEKVILRWDPNFDVSRRSVNWEDWQDEAAEYLSAVDDILQLVDELSIGSES 120

Query: 121 TEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDEF 180
           T+IVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFAS+YSEIDDEF
Sbjct: 121 TDIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASHYSEIDDEF 180

Query: 181 ESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYSTV 240
           ESF EESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIR+GYEKECVQVYSTV
Sbjct: 181 ESFSEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRSGYEKECVQVYSTV 240

Query: 241 RRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQIF 300
           RRDALDECL ILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLIL+GEKRLCDQIF
Sbjct: 241 RRDALDECLMILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILEGEKRLCDQIF 300

Query: 301 NGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAMV 360
           NGA+ESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALA VLPDLEAMV
Sbjct: 301 NGASESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALASVLPDLEAMV 360

Query: 361 SDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYVRLLV 420
           SDEFLI EAHGVLSGLGEAATGTF EFENAIE+ENSKKAMQNAEIHPLIRYVMNYVRLLV
Sbjct: 361 SDEFLIHEAHGVLSGLGEAATGTFVEFENAIENENSKKAMQNAEIHPLIRYVMNYVRLLV 420

Query: 421 DYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSKLYED 480
           DYSKT+NSLLED+EVQD+ N   NDD+LQL+STSPLARRLLMLLSSLESNL++KSKLYED
Sbjct: 421 DYSKTINSLLEDDEVQDMPNERHNDDNLQLESTSPLARRLLMLLSSLESNLMEKSKLYED 480

Query: 481 IAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLK 540
           IAMQ+IFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLK
Sbjct: 481 IAMQYIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLK 540

Query: 541 DEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPAY 600
           DEGI GSTSNASKVALKEKFKNFNAGFEEICRVQT+WKVSDAQLRDELIISVSEKVIPAY
Sbjct: 541 DEGISGSTSNASKVALKEKFKNFNAGFEEICRVQTAWKVSDAQLRDELIISVSEKVIPAY 600

Query: 601 RSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGSPAVSHHMRRKGT 652
           RSFLGRFRNQLESGRHSGKYIKYT +DLENSLS+LFEGSPA+SHH+RR GT
Sbjct: 601 RSFLGRFRNQLESGRHSGKYIKYTSDDLENSLSNLFEGSPAISHHLRRNGT 651

BLAST of Lcy03g020280 vs. TAIR 10
Match: AT5G50380.1 (exocyst subunit exo70 family protein F1 )

HSP 1 Score: 797.0 bits (2057), Expect = 1.2e-230
Identity = 429/692 (61.99%), Postives = 527/692 (76.16%), Query Frame = 0

Query: 1   MAATNTNVAS----------GVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSS 60
           MAAT T  AS          G +DRVLATAQQI+KSLNTPKEVREDMLLI SSFDNRLS+
Sbjct: 1   MAATTTTAASINAEAGGGGGGAEDRVLATAQQIMKSLNTPKEVREDMLLIFSSFDNRLSN 60

Query: 61  IGSMIGSDDSKTKNDT----LDAAEKVILRWDPNSDVSRR-------------SLNWEDS 120
           I + + ++  + +ND     L+AAE VI RWD  +D SR              SL++++S
Sbjct: 61  IKTAM-TNQEENQNDALVARLEAAESVIHRWDGGNDSSRHSSSSSGNYRSSSFSLSFDES 120

Query: 121 PDEAAEYLSAVDDILRLLEDLSIGSDSTEIVDRAENAIQMAMCRLESEFRHILIQSTVPL 180
           P+EA E+LSAVD+I+ LLEDLS   +  ++VDRA++A+QMAM +LE EFR ILI++TVPL
Sbjct: 121 PEEATEFLSAVDEIISLLEDLS-SENKPDMVDRADSALQMAMSQLEDEFRRILIRNTVPL 180

Query: 181 DAERLYGSIRRVHLSFASNYSEIDDEFESFGEESRSSGR------FHERGATIGEDSWVD 240
           DAERLYGS+RRV LSFA    ++ ++FE+FG  +   G       FHERG +IG D WVD
Sbjct: 181 DAERLYGSMRRVSLSFAD--GDVVEDFENFGLVADGDGSGSRRRLFHERGGSIGCDLWVD 240

Query: 241 LIHPDAAVDLSEIADRMIRAGYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDW 300
           LI+P A  DL EIA+RMIRAGYEKECVQVYS+VRRDALD+CL ILGVE+LSIEEVQK DW
Sbjct: 241 LINPTAVEDLKEIAERMIRAGYEKECVQVYSSVRRDALDDCLMILGVEKLSIEEVQKIDW 300

Query: 301 KFLDEKMKKWIQAVKITVRLILDGEKRLCDQIFNGANESKEICFNETAKGCVMQLLNFGE 360
           K +DEKMKKWIQAVKITVR++L GEK++CD+IF+ +  SKE+CFNET K CVMQLLNFGE
Sbjct: 301 KSMDEKMKKWIQAVKITVRVLLVGEKKICDEIFSSSESSKEVCFNETTKSCVMQLLNFGE 360

Query: 361 AVAIGKRSPEKLFRILDMYDALAGVLPDLEAMVSDEFLISEAHGVLSGLGEAATGTFAEF 420
           AVAIG+RS EKLFRILDMYDALA VL  LE MV+D F+ +E  GVL  LG+AA GTF EF
Sbjct: 361 AVAIGRRSSEKLFRILDMYDALANVLQTLEVMVTDCFVCNETKGVLEALGDAARGTFVEF 420

Query: 421 ENAIESENSKKAMQNAEIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDNDDD 480
           EN + +E SK+   N E+HP+IRYVMNY++L+VDY+ T+NSLLE  E   +      DD 
Sbjct: 421 ENNVRNETSKRPTTNGEVHPMIRYVMNYMKLIVDYAVTLNSLLESNESSGV----SGDDS 480

Query: 481 LQLKSTSPLARRLLMLLSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDSELAQL 540
            +    SPLA+R+L L++SLESNL DKSKLYED  +Q +F+MNNI YIV+KVKDSEL +L
Sbjct: 481 TE--EMSPLAKRILGLITSLESNLEDKSKLYEDGGLQHVFMMNNIYYIVQKVKDSELGKL 540

Query: 541 LGDNWLRRHSGQIRQYETSYLRASWSKVLSCLKDEGIGGSTS----------NASKVALK 600
           LGD+W+R+  GQIRQY T YLRASWS+VLS L+DE +GGS+S          N+SK+ALK
Sbjct: 541 LGDDWVRKRRGQIRQYATGYLRASWSRVLSALRDESMGGSSSGSPSYGQRSNNSSKMALK 600

Query: 601 EKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHS 650
           E+F+ FNA FEE+ R+QT+WKV D QLR+EL IS+SEKVIPAYR+F GR R+QLE GRH+
Sbjct: 601 ERFRGFNASFEELYRLQTAWKVPDPQLREELRISISEKVIPAYRAFFGRNRSQLEGGRHA 660

BLAST of Lcy03g020280 vs. TAIR 10
Match: AT1G72470.1 (exocyst subunit exo70 family protein D1 )

HSP 1 Score: 452.6 bits (1163), Expect = 5.4e-127
Identity = 269/629 (42.77%), Postives = 391/629 (62.16%), Query Frame = 0

Query: 56  GSDDSKTKNDTL---DAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLE 115
           G D  + +N ++   D+AEKVI+RWD  +    R     + P E   YL+AVD+I + + 
Sbjct: 9   GGDGGEGENCSVSGFDSAEKVIIRWDATASEEAREKMIFNDPQEVNLYLNAVDEIQKYV- 68

Query: 116 DLSIGSDSTEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERL-YGSIRRVHL---- 175
                S   EI +RA +AIQ+AM RLE EFR+IL+  + P++++ L   S    HL    
Sbjct: 69  -----SSGGEIENRANSAIQIAMARLEDEFRNILVSHSSPINSDSLMLSSSSSSHLEVDE 128

Query: 176 --SFASNYSEIDDEFESFGEE--SRSSGRFHERGATI----GEDSW---------VDLIH 235
             S ++N +E DD+ E    +   RS        AT+    G  S+         ++LI 
Sbjct: 129 DGSSSNNGNEEDDQQEEEETDLLKRSGSSASTGSATVRLPTGRGSYSRSTSSIREIELIP 188

Query: 236 PDAAVDLSEIADRMIRAGYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDWKFL 295
            ++ + LS IA RM+ AGY +EC+QVY +VR+ A+D     LG+E+LSI +VQ+ +W+ L
Sbjct: 189 IESVIHLSWIARRMVSAGYLRECIQVYGSVRKSAVDSSFRRLGIEKLSIGDVQRLNWEAL 248

Query: 296 DEKMKKWIQAVKITVRLILDGEKRLCDQIFN--GANESKEICFNETAKGCVMQLLNFGEA 355
           ++K+++WI+A KI VR++   EK LC+ +F   GA    E CF ET KG  +QL NF EA
Sbjct: 249 EQKIRRWIRAAKICVRVVFASEKLLCEHVFESVGAVNIHEACFMETVKGPAIQLFNFAEA 308

Query: 356 VAIGKRSPEKLFRILDMYDALAGVLPDLEA---MVSDEFLISEAHGVLSGLGEAATGTFA 415
           ++I +RSPEKLF+ILD++DAL  +LPD+E+   + S + +  +A  +LS L EAA G  +
Sbjct: 309 ISISRRSPEKLFKILDLHDALIELLPDIESVFDLKSSDSIRVQAAEILSRLAEAARGILS 368

Query: 416 EFENAIESENSKKAMQNAEIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDND 475
           EFENA+  E S+  +    IHPL RYVMNY+ L+ +Y  T+  L+  +  ++  + N  D
Sbjct: 369 EFENAVLREPSRVPVPGGTIHPLTRYVMNYISLISEYRPTLIDLIMSKPSRNATDSNTPD 428

Query: 476 DDLQ--LKSTSPLARRLLMLLSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDS- 535
            D      +  PLA  L+ ++  L+ NL  KSK Y++ A+  +F+MNN  YIV+K+K S 
Sbjct: 429 FDFSELENNKGPLALHLIWIIVILQFNLEGKSKYYKNAALSHLFIMNNAHYIVQKIKGSP 488

Query: 536 ELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLKDEGI---GGSTSNASKVALKEK 595
           EL +++GD +LR+ +G+ RQ  T Y RA+W KVL CL+DEG+   G  +S  S+ AL+E+
Sbjct: 489 ELREMIGDLYLRKLTGKFRQAATYYQRAAWVKVLYCLRDEGLHTKGSFSSGVSRSALRER 548

Query: 596 FKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSGK 649
           FK+FNA FEE+ RVQ+ W V D+QLR+EL IS+ EK+ PAYRSFLGRFR+ +ESG+H   
Sbjct: 549 FKSFNALFEEVHRVQSQWLVPDSQLREELKISILEKLSPAYRSFLGRFRSHIESGKHPEN 608

BLAST of Lcy03g020280 vs. TAIR 10
Match: AT3G14090.1 (exocyst subunit exo70 family protein D3 )

HSP 1 Score: 445.7 bits (1145), Expect = 6.6e-125
Identity = 257/609 (42.20%), Postives = 371/609 (60.92%), Query Frame = 0

Query: 67  LDAAEKVILRWD-PNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDSTE--- 126
           L+ AE++ILRWD   +D +R  + ++   DE   +L AVD+I R L  +S  S S+    
Sbjct: 15  LETAERIILRWDSAATDGARGKMIFQSDRDEVDRFLRAVDEIQRSLSSVSFSSSSSSAAT 74

Query: 127 ----IVD----RAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYS 186
               +VD    +A +AIQ+AM RLE EFR+IL+  T   + + L+     V  S      
Sbjct: 75  SAATVVDEHEVKANSAIQIAMARLEDEFRNILLSHTTTFEPDSLFLEEPSVSPSLTVEVG 134

Query: 187 E----IDDEFESFGEESRSSGRFHERGATIGEDSWV---DLIHPDAAVDLSEIADRMIRA 246
           E      +E E        S R   R ++    S +   DLI P+A  DL  I  RM+ A
Sbjct: 135 EDTTVTPEEEELNSPGGSGSSRLTRRRSSYRSTSSIREMDLISPEAVSDLRSIVQRMVAA 194

Query: 247 GYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRL 306
           GY +EC+QVY TVR+ A++     LG+ ++SI +VQ+ +W+ ++ K++KWI+A K+ +R+
Sbjct: 195 GYSRECIQVYGTVRKSAMEAIFKQLGIVKISIGDVQRLEWEVVEGKIRKWIRAAKVCIRV 254

Query: 307 ILDGEKRLCDQIFNG-ANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMY 366
           +   EKRLC+Q+F+G      E CF ET K   ++L  F EA++I +RSPEKLF+ILD++
Sbjct: 255 VFSSEKRLCEQLFDGICTAMDETCFMETVKASALRLFTFPEAISISRRSPEKLFKILDLH 314

Query: 367 DALAGVLPDLEAMV---SDEFLISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNA 426
           DAL  +LPD+EA+    S + + ++A  + S L EAA G  +EFENA+  E S   +   
Sbjct: 315 DALTDMLPDIEAIFDSDSSDAIRAQAVEIQSRLAEAARGILSEFENAVLREPSIVPVPGG 374

Query: 427 EIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLML 486
            IHPL RYVMNY+ ++ DY +T++ L+             + D  +L S SPL   L+ L
Sbjct: 375 TIHPLTRYVMNYIVMISDYKQTLDDLIMSNPSTGSDPNTPDMDFTELDSKSPLDLHLIWL 434

Query: 487 LSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDS-ELAQLLGDNWLRRHSGQIRQ 546
           +  L  NL +KSK Y D ++  IF+MNNI YIV+KVK S EL +++GD++LR+ +G  R 
Sbjct: 435 IVVLHFNLEEKSKHYRDTSLAHIFIMNNIHYIVQKVKRSPELREMIGDHYLRKLTGIFRH 494

Query: 547 YETSYLRASWSKVLSCLKDEGI---GGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKV 606
             T+Y RA+W +VL+ L+DEG+   G  +S  S+ AL+E+FK FN  FEE+ R Q++W V
Sbjct: 495 AATNYQRATWVRVLNSLRDEGLHVSGSFSSGVSRSALRERFKAFNTMFEEVHRTQSTWSV 554

Query: 607 SDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGS 649
            DAQLR+EL IS+SE +IPAYRSFLGRFR  +ESGRH   Y+KY+ ED+E  + D FEG 
Sbjct: 555 PDAQLREELRISLSEHLIPAYRSFLGRFRGHIESGRHPENYLKYSVEDIETIVLDFFEGY 614

BLAST of Lcy03g020280 vs. TAIR 10
Match: AT1G54090.1 (exocyst subunit exo70 family protein D2 )

HSP 1 Score: 442.2 bits (1136), Expect = 7.3e-124
Identity = 264/629 (41.97%), Postives = 377/629 (59.94%), Query Frame = 0

Query: 55  IGSDDSKTKNDTLDAAEKVILRWD-PNSDVSRRSLNWEDSPD--EAAEYLSAVDDILRLL 114
           + + +++  +  L+ AEK+ILRWD   S+ ++ +L ++   D  E   YL AVD++ R +
Sbjct: 1   MATPETRGVDSDLETAEKIILRWDSTTSEEAKENLIFQSGGDREEVDRYLKAVDEVQRHI 60

Query: 115 EDLSIGSDSTEIVDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFA- 174
             +SI        D  +  IQ+AM RLE E R+ILI  T   + + L         SFA 
Sbjct: 61  SSISIS-------DEVKATIQIAMARLEDELRNILISQTSTFEPDSLLLDSSLSSSSFAS 120

Query: 175 SNYSEIDDEFESFGEE----------------SRSSGRFHERGATIGEDSW-----VDLI 234
           S+ +E++D+    G E                S S  R   R       S      +DL+
Sbjct: 121 SSRTELEDDTCDDGNEEEEQQQVDLVLPDGSGSDSGSRRLSRSRRSNSKSTSSIREIDLV 180

Query: 235 HPDAAVDLSEIADRMIRAGYEKECVQVYSTVRRDALDECLAILGVERLSIEEVQKSDWKF 294
            P+A  DL  IA RMI AGY +ECVQVY  VR+ A++     LG+ +L I +VQ+ DW+ 
Sbjct: 181 TPEAVSDLRSIAQRMIGAGYSRECVQVYGNVRKSAMEMIFKQLGIVKLGIGDVQRLDWEA 240

Query: 295 LDEKMKKWIQAVKITVRLILDGEKRLCDQIFNGANESKEICFNETAKGCVMQLLNFGEAV 354
           ++ K+++WI+A K+ VR++   EKRLC+QIF G  E  E CF E  K   +QL NF EA+
Sbjct: 241 VEVKIRRWIRAAKVCVRVVFASEKRLCEQIFEGTME--ETCFMEIVKTSALQLFNFPEAI 300

Query: 355 AIGKRSPEKLFRILDMYDALAGVLPDLEAMV---SDEFLISEAHGVLSGLGEAATGTFAE 414
           +I +RSPEKLF+ILD++DA+  +LPD+E +    S E ++ +A  + S L EAA G   E
Sbjct: 301 SISRRSPEKLFKILDLHDAITDLLPDMEEIFDSSSSESILVQATEIQSRLAEAARGILTE 360

Query: 415 FENAIESENSKKAMQNAEIHPLIRYVMNYVRLLVDYSKTMNSLLEDEEVQDLRNVND-ND 474
           FENA+  E S   +    IHPL RYVMNY+ L+ DY +T+  L+  +  + L+  ND ND
Sbjct: 361 FENAVFREPSVVPVPGGTIHPLTRYVMNYLNLISDYRETLIDLVMTKPCRGLKCTNDRND 420

Query: 475 DDL---QLKSTSPLARRLLMLLSSLESNLVDKSKLYEDIAMQFIFLMNNILYIVKKVKDS 534
            D+   +L+  SPLA  ++  +  L+ NL +KS  Y D  +  IF+MNN+ YIV+KVK S
Sbjct: 421 PDMDISELEGISPLALHMIWTMVMLQFNLEEKSLHYRDEPLSHIFVMNNVHYIVQKVKSS 480

Query: 535 -ELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVLSCLKDEGI---GGSTSNASKVALKE 594
            EL +L+GD +LR+ +G  RQ  T Y RA+W +VL+ L+DEG+   G  +S  SK AL+E
Sbjct: 481 PELMELIGDKYLRKLTGIFRQAATKYQRATWVRVLNSLRDEGLHVSGSFSSGVSKSALRE 540

Query: 595 KFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKVIPAYRSFLGRFRNQLESGRHSG 648
           +FK FN  FEE+ R+Q++W V D QLR+EL IS+SE +IPAYRSFLGRFR  +ESGRH  
Sbjct: 541 RFKAFNTMFEEVHRIQSTWSVPDTQLREELRISLSEHLIPAYRSFLGRFRGHIESGRHPE 600

BLAST of Lcy03g020280 vs. TAIR 10
Match: AT5G58430.1 (exocyst subunit exo70 family protein B1 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-108
Identity = 226/643 (35.15%), Postives = 377/643 (58.63%), Query Frame = 0

Query: 8   VASGVDDRVLATAQQIVKSLNTPKEVREDMLLILSSFDNRLSS---IGSMIGSDDS--KT 67
           +A   ++++LA A+ I K+L   + + +D+L I S+FD R S         G D S   T
Sbjct: 1   MAENGEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRFSREKLAEGQAGEDGSGVAT 60

Query: 68  KNDTLDAAEKVILRWDPNSDVSRRSLNWEDSPDEAAEYLSAVDDILRLLEDLSIGSDSTE 127
               L++ +  I R+     V+     W D  D AA +L  +D+++ ++ + S  +    
Sbjct: 61  LERALNSIDGQISRF-----VAADQPIWADPADSAA-FLDTIDELVAIIREWSPMASEKP 120

Query: 128 I---VDRAENAIQMAMCRLESEFRHILIQSTVPLDAERLYGSIRRVHLSFASNYSEIDDE 187
           I   + RA++ +Q AM R+E EFR ++ +           G    ++  F S   E DD 
Sbjct: 121 IGICLTRADDMMQQAMFRIEEEFRSLMERGAESFGLNP-QGDAGAMNHRFDSEEEEDDDR 180

Query: 188 FESFGEESRSSGRFHERGATIGEDSWVDLIHPDAAVDLSEIADRMIRAGYEKECVQVYST 247
             + G++     +          D  +D +      DL E+A RM+ AG+ K C  VYS+
Sbjct: 181 DFNNGDDI----QIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSS 240

Query: 248 VRRDALDECLAILGVERLSIEEVQKSDWKFLDEKMKKWIQAVKITVRLILDGEKRLCDQI 307
            RR+ L+E ++ LG+++LSIEEV K  W+ L++++ +WI+A  + +R++   E+RLCD++
Sbjct: 241 CRREFLEESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRV 300

Query: 308 FNGANESKEICFNETAKGCVMQLLNFGEAVAIGKRSPEKLFRILDMYDALAGVLPDLEAM 367
           F G + + ++ F E  +G  +QLLNF +A+AIG RSPE+LF++LD+++ +  ++P+ E++
Sbjct: 301 FFGFSSAADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESV 360

Query: 368 VSDEF---LISEAHGVLSGLGEAATGTFAEFENAIESENSKKAMQNAEIHPLIRYVMNYV 427
            SD+F   L +EA  +   LGEA  G F E EN I  + +K A+    +HP+ RYVMNY+
Sbjct: 361 FSDQFCSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYL 420

Query: 428 RLLVDYSKTMNSLLEDEEVQDLRNVNDNDDDLQLKSTSPLARRLLMLLSSLESNLVDKSK 487
           R      +T+  + E+             + +  K ++ L  ++  ++  LESNL  KSK
Sbjct: 421 RAACRSRQTLEQVFEE------------SNGVPSKDSTLLTVQMSWIMELLESNLEVKSK 480

Query: 488 LYEDIAMQFIFLMNNILYIVKKVKDSELAQLLGDNWLRRHSGQIRQYETSYLRASWSKVL 547
           +Y+D A+ ++FLMNN  YIV+KVKD +L  LLGD+W+R+H+ +++QY  +Y R+SW+K+L
Sbjct: 481 VYKDPALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKML 540

Query: 548 SCLKDEGIGGSTSNASKVALKEKFKNFNAGFEEICRVQTSWKVSDAQLRDELIISVSEKV 607
             LK +      +   K  +KEK K FN  F+EIC+V ++W V D QL++EL IS++  +
Sbjct: 541 GLLKVDNTAAGMNGLGK-TMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLL 600

Query: 608 IPAYRSFLGRFRNQLESGRHSGKYIKYTPEDLENSLSDLFEGS 640
           +PAY SF+GRF+N  + G+++ KYIKY  ED+E  +++LF+G+
Sbjct: 601 VPAYGSFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKGT 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGH92.7e-10735.15Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LZD36.9e-9533.74Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
Q9LMJ43.7e-8833.07Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
Q9FNR34.8e-8833.80Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=... [more]
F4KG586.1e-7533.95Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KKZ60.0e+0094.03Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495521... [more]
A0A6J1EKA60.0e+0093.72Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114351... [more]
A0A6J1E1E80.0e+0092.79Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114297... [more]
A0A6J1JIF90.0e+0092.64Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111484744... [more]
A0A5D3E0I40.0e+0091.26Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
XP_023001365.10.0e+0094.03exocyst complex component EXO70B1-like [Cucurbita maxima][more]
XP_023529911.10.0e+0093.72exocyst complex component EXO70B1-like [Cucurbita pepo subsp. pepo][more]
KAG6587413.10.0e+0093.57Exocyst complex component EXO70B1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022928239.10.0e+0093.72exocyst complex component EXO70B1-like [Cucurbita moschata][more]
XP_038877265.10.0e+0092.63exocyst complex component EXO70B1-like [Benincasa hispida] >XP_038877266.1 exocy... [more]
Match NameE-valueIdentityDescription
AT5G50380.11.2e-23061.99exocyst subunit exo70 family protein F1 [more]
AT1G72470.15.4e-12742.77exocyst subunit exo70 family protein D1 [more]
AT3G14090.16.6e-12542.20exocyst subunit exo70 family protein D3 [more]
AT1G54090.17.3e-12441.97exocyst subunit exo70 family protein D2 [more]
AT5G58430.11.9e-10835.15exocyst subunit exo70 family protein B1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 275..634
e-value: 3.3E-125
score: 418.0
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 50..650
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 73..645
e-value: 2.5E-182
score: 609.3
NoneNo IPR availablePANTHERPTHR12542:SF146EXOCYST SUBUNIT EXO70 FAMILY PROTEINcoord: 50..650
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 65..637

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g020280.1Lcy03g020280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
biological_process GO:0015031 protein transport
cellular_component GO:0000145 exocyst