Lcy03g020020 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g020020 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.9e-83
Identity = 168/423 (39.72%), Postives = 256/423 (60.52%), Query Frame = 0

Query: 6   IHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQLK 65
           + ++ +E+I PSSPTP  L+  KLS LDQ+  + + PII FY    +   DR   S  LK
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENLK 64

Query: 66  KSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHRLLPF 125
           +SLS +LT FYP AG ++    SVDCND GV + EARV   LSE +KN     L++ LPF
Sbjct: 65  RSLSTVLTQFYPLAGRININS-SVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLPF 124

Query: 126 EPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINRGDS 185
           +PY    E  + +D+ LAV+++ F+CGG AI +CI+HK+ D  S   F+  W A  + ++
Sbjct: 125 QPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQEET 184

Query: 186 DQVSEPLFD-GAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAGSLE- 245
           D + +P FD G+ +FPP E   +   + + ++ IV KRFVFDK  + AL+ + A+ + E 
Sbjct: 185 D-IVQPNFDLGSHHFPPME--SIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSATEV 244

Query: 246 KPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLWWFA 305
           K  +RV+ V A +WK+F+++  R++ + +    A  AVNLR +M+PP P ++ GN+   A
Sbjct: 245 KNSSRVQIVIAVIWKQFIDVT-RAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGNIATMA 304

Query: 306 IAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGEVEF 365
            AVAE   +K    LV  ++ ++ +ID + V+ LQ+ GV    +    +         E 
Sbjct: 305 YAVAEE--DKDFSDLVGPLKTSLAKIDDEHVKELQK-GVTYLDYEAEPQ---------EL 364

Query: 366 CNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDMASF 425
            +F+SWCR   Y+ DFGWGKP  VC+ + P KN+V LM TR+ DG+EAW+++ E++M+  
Sbjct: 365 FSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMSML 410

Query: 426 ERD 427
             D
Sbjct: 425 SSD 410

BLAST of Lcy03g020020 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 282.3 bits (721), Expect = 9.0e-75
Identity = 156/415 (37.59%), Postives = 238/415 (57.35%), Query Frame = 0

Query: 9   IGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQLKKSL 68
           + +E+ILPSSPTP  L+  K+S LDQ+  + + P I FY    +   D    S  LK+SL
Sbjct: 8   VSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTSQHLKQSL 67

Query: 69  SEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKN-PDFKVLHRLLPFEP 128
           S++LT FYP AG ++    SVDCND GV + EARV   LS+ ++N  + + L + LP   
Sbjct: 68  SKVLTHFYPLAGRINVNS-SVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLPSAA 127

Query: 129 YANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINRGDSDQ 188
           Y    + E+  DV LAV+++ F+CGG AI + ++HK+ D  S   F+  W A  RG+++ 
Sbjct: 128 YPG-GKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGETEI 187

Query: 189 VSEPLFD-GAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAGSLEKPP 248
           V  P FD  A++FPP  +      ++V ++ +V KRFVFDK  I ALR +A++ S EK  
Sbjct: 188 VL-PNFDLAARHFPP--VDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSASEEKNF 247

Query: 249 TRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLWWFAIAV 308
           +RV+ V A++WK  +++  R++   +  F  V AVNLR +M+PPLP  + GN+     A 
Sbjct: 248 SRVQLVVAYIWKHVIDVT-RAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGNIATLLFAA 307

Query: 309 AEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGEVEFCNF 368
            +   +K    L+  +R ++++ + D    L +     +                E  +F
Sbjct: 308 VDAEWDKDFPDLIGPLRTSLEKTEDDHNHELLKGMTCLYELEPQ-----------ELLSF 367

Query: 369 TSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDMA 422
           TSWCR   Y+ DFGWGKP   C+ + P +N  +LM TR GDG+EAW+ + E++MA
Sbjct: 368 TSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEMA 405

BLAST of Lcy03g020020 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.8e-65
Identity = 153/437 (35.01%), Postives = 239/437 (54.69%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MA + + ++    I PSSPTP  LR   LS +DQ   S  T + FFY    NP  +    
Sbjct: 1   MASKMVEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY--TYNPSHESSKI 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
              LK SLS+ L  +Y FAG L    Y +DCND GVE+ E R+   +++++K        
Sbjct: 61  GDLLKNSLSKTLVSYYQFAGRLIENDY-IDCNDEGVEFVEVRIHGRMNDILKRGKSFATD 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
            +LP    A      +  D +L V+L+ FDCGG+AI    +HK+ DG S  +F+++WA+ 
Sbjct: 121 LVLPTRIIA------LHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASS 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
               +     PL      FPP++   L    +V   + + KRFVF    I  L+ +A   
Sbjct: 181 TSLSTFHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEY 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
            ++K P+RVE V+AFL     +     +   +K +A + AVNLR  +   LP NS GN++
Sbjct: 241 GIQK-PSRVEVVTAFL----CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIF 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHM--WSKMGKNGQAAG 360
            F   + + G++ +   L+ ++RN  +++  +++ + ++    S M   +K       + 
Sbjct: 301 SFYFCINDEGMDNQFSALISKLRNGKQKLLENII-SKEKLTYESQMQELTKCLDQLNISS 360

Query: 361 GEVEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPY-KNVVVLMTTRDGDGIEAWVTLEE 420
            +  FC  +SWCRFPVY+ DFGWGKP  V SP +P+ K++++LM + +GDGIEA +T+EE
Sbjct: 361 LDTYFC--SSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEE 417

Query: 421 EDMASFERDSELISFTY 435
           + MA+FE++ EL SF Y
Sbjct: 421 KKMAAFEKNEELRSFAY 417

BLAST of Lcy03g020020 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 6.3e-60
Identity = 156/469 (33.26%), Postives = 228/469 (48.61%), Query Frame = 0

Query: 6   IHVIGDEVILPSSPTPPPLRNLKLSLLDQISP-SNYTPIIFFY---AAVENPGADRFSGS 65
           + VI  E I P++PTP  L+N  LSLLDQ  P   Y PII FY   AA     ++     
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 66  SQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHR 125
             LK SLS+ L  FYP AG +  +   VDC+D+G+ + + ++   + E +  PD   L +
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRM-IDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVP-LSQ 128

Query: 126 LLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAIN 185
           LLP E    +    + ++ ++ V++N+FDCGG AI   ++HK+ D  + + F+R WA+  
Sbjct: 129 LLPSE----VVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTT 188

Query: 186 RGD---------SDQVSEPLFDGAKYFPP----------KEIPGLKRSKVVINDKIVTKR 245
           +           +DQ   P FD A  FPP           EIP     +   +DK V+KR
Sbjct: 189 KTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKR 248

Query: 246 FVFDKANIAALRQEAAA----GSLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAV 305
           FVFD A I ++R++           +  TRVE V++ +WK  M+  P         F  V
Sbjct: 249 FVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKSTPAG-------FLPV 308

Query: 306 --HAVNLRGKMDPPLPPNSSGNL--------------WWFAIAVAEGGIEKRHGYLVKQI 365
             HAVNLR KMDPPL   S GNL                 A+             ++ ++
Sbjct: 309 VHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHEL 368

Query: 366 RNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAA---GGEVEFCNF--TSWCRFPVYEA 425
            + I ++ S++ +     G    +         A+     +VE  NF  +SWCR  +YE 
Sbjct: 369 HDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWCRMGLYEI 428

BLAST of Lcy03g020020 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.2e-60
Identity = 166/472 (35.17%), Postives = 224/472 (47.46%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAA---VENPGADR 60
           M+   + VI  E I P +PTP  LRN  +SLLDQ S   Y PII FY A     + G+  
Sbjct: 4   MSSAAVEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKH 63

Query: 61  FSGSSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFK 120
                 LK+SLSE L  FYP AG +  +  +VDCND G+++ E R+   + + +   D  
Sbjct: 64  HDDLHLLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAH 123

Query: 121 VLHRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREW 180
            L  LLP E    +A     ++  + V++N+FDCGG AI  CI++K+ D  +   F+R  
Sbjct: 124 -LSLLLPSE----VASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSL 183

Query: 181 AA----INRGDS------DQVSEPLFDGAKYFPPKE-------IPGLKRSKVVINDKIVT 240
           A       RG S      +Q   P FD    FPP E        P    + V   DK+V+
Sbjct: 184 AGTTNIARRGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSV---DKLVS 243

Query: 241 KRFVFDKANIAALRQEAAAGSLEK----PPTRVEAVSAFLWKRFMEINPRSEQENEKIFA 300
           KRFVFD A I + R++  +   +K     PTRVE VSA +WK  ++  P        I  
Sbjct: 244 KRFVFDAAKITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPG-----SIST 303

Query: 301 AVHAVNLRGKMDPPLPPNSSGNLWWFAIAVAEGGIEKRHGYLVKQIRNAIKE----ID-- 360
             HA+N R KMDPPL   S GNL     AV             K++ +   E    +D  
Sbjct: 304 VTHAMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDEL 363

Query: 361 SDLVRNLQQ--------AGVPSHMWSKM--GKNGQAA-----GGEVEFCNFTSWCRFPVY 420
           SD V  L++         G    +  K   G +   A       +V     TSWC+F  Y
Sbjct: 364 SDFVALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFY 423

Query: 421 EADFGWGKPSWVCSP---SRPYKNVVVLMTTRDGDGIEAWVTLEEEDMASFE 425
           EADFGWG P WV +       YKN+V +   + G+GIE WV   E+DM  FE
Sbjct: 424 EADFGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWVNFLEDDMTKFE 461

BLAST of Lcy03g020020 vs. ExPASy TrEMBL
Match: A0A6J1C4D5 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007275 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.2e-232
Identity = 390/434 (89.86%), Postives = 413/434 (95.16%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAI NI V  DE+ILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVE+ GADRFSG
Sbjct: 1   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDLGADRFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLSTEGYSVDCND+GVEYSEARV C L EVV++PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKV++NDKIVTKRFVFDKANIAALR+EAAAG
Sbjct: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEIN  SEQENEKI+AA+HAVNLRGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  ++KRH YLVKQIRNAI+EIDSDLVRNLQQAGVP HMWSK GK+G  A GE
Sbjct: 301 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSDLVRNLQQAGVPPHMWSKTGKHGHPAAGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 420

Query: 421 ASFERDSELISFTY 435
           ASF++DSEL+++TY
Sbjct: 421 ASFQQDSELVTYTY 434

BLAST of Lcy03g020020 vs. ExPASy TrEMBL
Match: A0A6J1I7Z0 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111472185 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 7.1e-232
Identity = 396/434 (91.24%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI DE+I PSSPTPP  RNLK SLLDQISPSNYTPIIFFYAAVENPGADRFSG
Sbjct: 1   MAIELIQVIADEIISPSSPTPPSRRNLKFSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP SEQ  EK+FAAVHAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTL+EEDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFT+
Sbjct: 421 ELFQRDSELLSFTF 432

BLAST of Lcy03g020020 vs. ExPASy TrEMBL
Match: A0A6J1EY64 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111439839 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.7e-231
Identity = 396/434 (91.24%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI DE+I PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVENPGAD FSG
Sbjct: 1   MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADWFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP SEQ  EK+FAAVHAVN+RGKMDPPLPP+SSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPSSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTL+EEDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFTY
Sbjct: 421 ELFQRDSELLSFTY 432

BLAST of Lcy03g020020 vs. ExPASy TrEMBL
Match: A0A6J1CAK2 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111008950 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 1.1e-128
Identity = 241/432 (55.79%), Postives = 299/432 (69.21%), Query Frame = 0

Query: 6   IHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQLK 65
           + +I  E I PSSPTPP  R LKLSLLDQ++P +YTP++FFY A +    D      +LK
Sbjct: 5   VEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRKLK 64

Query: 66  KSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNP-DFKVLHRLLP 125
           +SL+E LT FY  AGTL  E Y V+CND GVE+SEARV C L++V+  P D   LHRLLP
Sbjct: 65  ESLAETLTRFYLLAGTL-VEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLLP 124

Query: 126 FEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINRGD 185
           F+P A L      RD ILAVR N FDCGGV IALC+THK+VDG SAT F + WAA  RGD
Sbjct: 125 FDPDAVL-----DRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGD 184

Query: 186 SDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAGSL-- 245
            +    P FD A+ FPP EI G  + +  + DKIVTKRF+F K+NIAAL+QEA + +   
Sbjct: 185 HELTVVPTFDAAELFPPMEIRGGNKRQSRM-DKIVTKRFIFSKSNIAALKQEANSDAFLN 244

Query: 246 EKPPTRVEAVSAFLWKRFMEI-NPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLWW 305
           ++PP+RVE VSAF+WKRFM +   +      K FA +HAVN+R ++ PPLPP+S GNLWW
Sbjct: 245 QRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWW 304

Query: 306 FAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGEV 365
           FA+A A    EK + +LV +IRNAI EID + V  LQ A        KMG+      GEV
Sbjct: 305 FAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQ--MVYSGEV 364

Query: 366 EFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDMA 425
           E  +FTSWC FP+YE+DFGWG+P+WVCSP RPYKNVV+L+ T DG+GIEAWV LEE+DM 
Sbjct: 365 ELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMP 424

Query: 426 SFERDSELISFT 434
            FE D EL++FT
Sbjct: 425 VFESDPELLAFT 427

BLAST of Lcy03g020020 vs. ExPASy TrEMBL
Match: A0A7N2MLI3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 2.5e-128
Identity = 230/432 (53.24%), Postives = 308/432 (71.30%), Query Frame = 0

Query: 3   IENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSS 62
           + N+ +I  E+I PSSPTP  L N KLS LDQI+P  Y PII FY + +    D F  S 
Sbjct: 2   VMNVDIISKEIIKPSSPTPHHLSNFKLSFLDQIAPPIYIPIILFYDSEKFVDVDPFEISH 61

Query: 63  QLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHRL 122
            LKKSL+E LT +YP AGT   E + ++CND+GV+Y +AR+ C+LS+VV NP  K L +L
Sbjct: 62  SLKKSLAETLTRYYPLAGTPINEDFYINCNDQGVDYIQARIPCNLSQVVDNPKGKELMQL 121

Query: 123 LPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINR 182
           LPFEPY   +  E G++V+LAV+ N+F+CGGVAIA+C++HK+ DG SA  FV  WA ++R
Sbjct: 122 LPFEPYH--SRTEFGKEVLLAVQYNMFECGGVAIAVCVSHKIADGTSAVTFVNAWAGMSR 181

Query: 183 GDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAA--G 242
           G+S+ +S P FD A +FPP++I G   S  V  + IVT+RFVF+K++IA LR+EA+A  G
Sbjct: 182 GESEIIS-PSFDAAIHFPPRDITGSMLSGAVSKENIVTRRFVFNKSSIATLREEASAAFG 241

Query: 243 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 302
             +K P+RVEA+SAF+W+RFM ++   + +  K+FAA+HAVNLR +M P LP +S GNLW
Sbjct: 242 PEDKGPSRVEAISAFIWRRFMAMSRAKQGKKPKLFAALHAVNLRERMVPQLPVHSFGNLW 301

Query: 303 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 362
              IA     IEK + +LV Q+RNAI EID+D ++ L+      H+      + Q   G 
Sbjct: 302 RIVIAALPVEIEKDYRFLVSQLRNAIMEIDADFLKKLKDD--DGHLDFIKNTSKQFDHGI 361

Query: 363 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 422
           +EFCNFTSWCRFPVYE DFG GKP+WVCSPSRP+KNVVV+++T+DGDGIE WV ++EEDM
Sbjct: 362 IEFCNFTSWCRFPVYEVDFGMGKPTWVCSPSRPFKNVVVMISTKDGDGIETWVNMKEEDM 421

Query: 423 ASFERDSELISF 433
           A FE D EL+S+
Sbjct: 422 ALFEHDQELLSY 428

BLAST of Lcy03g020020 vs. NCBI nr
Match: KAG6587429.1 (CBBY-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815.8 bits (2106), Expect = 1.7e-232
Identity = 398/434 (91.71%), Postives = 409/434 (94.24%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI DE+I PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG
Sbjct: 347 MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 406

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 407 SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 466

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 467 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 526

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 527 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 586

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP SEQ  EK+FAAVHAVN+RGKMDPPLPPNSSGNLW
Sbjct: 587 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 646

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 647 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 706

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTL+EEDM
Sbjct: 707 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 766

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFTY
Sbjct: 767 ELFQRDSELLSFTY 778

BLAST of Lcy03g020020 vs. NCBI nr
Match: XP_022135273.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 813.9 bits (2101), Expect = 6.6e-232
Identity = 390/434 (89.86%), Postives = 413/434 (95.16%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAI NI V  DE+ILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVE+ GADRFSG
Sbjct: 1   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDLGADRFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLSTEGYSVDCND+GVEYSEARV C L EVV++PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKV++NDKIVTKRFVFDKANIAALR+EAAAG
Sbjct: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEIN  SEQENEKI+AA+HAVNLRGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  ++KRH YLVKQIRNAI+EIDSDLVRNLQQAGVP HMWSK GK+G  A GE
Sbjct: 301 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSDLVRNLQQAGVPPHMWSKTGKHGHPAAGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 420

Query: 421 ASFERDSELISFTY 435
           ASF++DSEL+++TY
Sbjct: 421 ASFQQDSELVTYTY 434

BLAST of Lcy03g020020 vs. NCBI nr
Match: XP_022973602.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 812.8 bits (2098), Expect = 1.5e-231
Identity = 396/434 (91.24%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI DE+I PSSPTPP  RNLK SLLDQISPSNYTPIIFFYAAVENPGADRFSG
Sbjct: 1   MAIELIQVIADEIISPSSPTPPSRRNLKFSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP SEQ  EK+FAAVHAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTL+EEDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFT+
Sbjct: 421 ELFQRDSELLSFTF 432

BLAST of Lcy03g020020 vs. NCBI nr
Match: XP_022933091.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 810.8 bits (2093), Expect = 5.6e-231
Identity = 396/434 (91.24%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI DE+I PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVENPGAD FSG
Sbjct: 1   MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADWFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP SEQ  EK+FAAVHAVN+RGKMDPPLPP+SSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPSSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTL+EEDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFTY
Sbjct: 421 ELFQRDSELLSFTY 432

BLAST of Lcy03g020020 vs. NCBI nr
Match: XP_023531342.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.8 bits (2080), Expect = 1.8e-229
Identity = 393/434 (90.55%), Postives = 406/434 (93.55%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           MAIE I VI D++I PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG
Sbjct: 1   MAIEFIQVIADQIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLH 120
           S QLKKSLSEILTLFYPFAGTLS+ GYSVDCNDRGVEYSEARVGC+LSEVV+NPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180
           RLLPFEPYAN  EPEI RDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIERDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAG 240
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKVV NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 241 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 300
           SLEKPPTRVEAVSAFLWKRFMEINP S Q  EK+FAAVHAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSAQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 301 WFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAGGE 360
           WFAIAVAE  IEKRH YLV QIRNAI+EIDSDLVR LQ AGVPSHMWSKMGK+G AAGGE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEEEDM 420
           VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIE WVTL+EEDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEGWVTLKEEDM 420

Query: 421 ASFERDSELISFTY 435
             F+RDSEL+SFT+
Sbjct: 421 ELFQRDSELLSFTH 432

BLAST of Lcy03g020020 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.6e-87
Identity = 177/435 (40.69%), Postives = 262/435 (60.23%), Query Frame = 0

Query: 6   IHVIGDEVILPS--SPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQ 65
           I V   E++ PS  +   PP  +  LS LDQ++P  + P +FFY    N  +D+   S  
Sbjct: 5   ITVTSQELVKPSPRNLNHPPCHH-HLSFLDQLAPPIFMPFLFFYHNKTNL-SDK-ERSDH 64

Query: 66  LKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHRLL 125
           +K SLSEIL L+YP AG +   G  V CND GV + EA+  C++S++++NP+   L++L 
Sbjct: 65  IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNKLH 124

Query: 126 PFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINRG 185
           PFE +      E+  DV L V+L  F+CGG+A+ + ++HKL D  S  +FV  WAA  RG
Sbjct: 125 PFEFH------EVS-DVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARG 184

Query: 186 DSDQVSEPLFDGAKYFPPKEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAAGSLE 245
            +D++  P FD AK FPP +I  L  +  +  + IVT+RFVF ++++ +LR E  +G+ +
Sbjct: 185 QTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLR-ERFSGNKK 244

Query: 246 KPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLWWFA 305
              TRVE +S F+W RFM  +   + +  KI+  +H VNLR + DP +P N  GN+  F+
Sbjct: 245 IRATRVEVLSVFIWSRFM-ASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFS 304

Query: 306 IAVAEGGI---EKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQAAG-- 365
           + V    I   ++    LV Q+R  I++ID+  V+ LQ+     +       N QA+G  
Sbjct: 305 VTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQE----DNRGHLEFLNKQASGFV 364

Query: 366 -GEVEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEE 425
            GE+   +FTS C+FPVYEADFGWGKP WV S    YKN+V  + T++GDGIEAW+ L++
Sbjct: 365 NGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQ 423

Query: 426 EDMASFERDSELISF 433
            DM+ FE D EL+ +
Sbjct: 425 NDMSRFEADEELLRY 423

BLAST of Lcy03g020020 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-77
Identity = 169/435 (38.85%), Postives = 248/435 (57.01%), Query Frame = 0

Query: 6   IHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQLK 65
           + V+  ++I PSSPTP  L+  KLSLL+Q+ P+ + P++FFY+A  N           LK
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSA--NNSIKPTEQLQMLK 62

Query: 66  KSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHRLLPF 125
           KSLSE LT FYP AG L     S+DCND G ++ EARV   LS ++  P    L +L+P 
Sbjct: 63  KSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIP- 122

Query: 126 EPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI-NRGD 185
                  +    R  +L  + + F+CG ++I +CI+HKL D  S  +F++ WAAI +RG 
Sbjct: 123 ----TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 182

Query: 186 SDQVSEPLFDGAKYFPPKEIPGLKRSKVV----INDKIVTKRFVFDKANIAALRQEAAAG 245
              +  P+FD  K FPP        + VV    + ++ ++KRF+FD ++I AL+ +A++ 
Sbjct: 183 IKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 242

Query: 246 SLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNLW 305
            + + PTRVEAVSA +WK  M+   R+     K     ++V+LR ++ PP   NS GNL 
Sbjct: 243 EVNQ-PTRVEAVSALIWKSAMKAT-RTVSGTSKPSILANSVSLRSRVSPPFTKNSIGNLV 302

Query: 306 WFAIAVAEGGI-EKRHGYLVKQIRNAIKEI-DSDLVRNLQQAGVPSHMWSKMGKNG-QAA 365
            +  A AE GI + +   LV +IR A +   D  + + +        + S   + G   A
Sbjct: 303 SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMIA 362

Query: 366 GGEVEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLEE 425
            G+ +F  F+S CRF +YE DFGWGKP WV  PS   KN+V L+ T++  GIEAWV L E
Sbjct: 363 SGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLNE 422

Query: 426 EDMASFERDSELISF 433
           ++M  FE+D EL+ F
Sbjct: 423 QEMNLFEQDRELLQF 427

BLAST of Lcy03g020020 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.8e-71
Identity = 164/440 (37.27%), Postives = 245/440 (55.68%), Query Frame = 0

Query: 6   IHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSGSSQLK 65
           +  I  E+I PSSPTP  L+ L+LS+ D I P  YT    FY   +N    +   S +LK
Sbjct: 8   VETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY--TKNDLISQEHTSHKLK 67

Query: 66  KSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARV-GCDLSEVVKNPDFKVLHRLLP 125
            SLSE LT FYP AG ++  G +VDC D G  + +ARV  C L+E +K PDF  L +LLP
Sbjct: 68  TSLSETLTKFYPLAGRIT--GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLP 127

Query: 126 FEPYANLAEPEIGRDV--ILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAINR 185
            +   N   P +      +L V+   F CGG+AI +CITHK+ D  S + F+R WAA  R
Sbjct: 128 LDVVDN---PYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 187

Query: 186 GDSD--QVSEPLFDGAKYFPP-KEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEAAA 245
           G++D   +  P+F GA ++PP  E   L   +       +TKRFVF+ + +  LR +AA+
Sbjct: 188 GENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAAS 247

Query: 246 GSLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSGNL 305
                 PTRVE+V+A +WK F+  +  +  +++ +   V   NLR K+   L  +S GNL
Sbjct: 248 EETVDQPTRVESVTALIWKCFVASSKTTTCDHKVL---VQLANLRSKIPSLLQESSIGNL 307

Query: 306 WWFAIAVAEG-GIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGK----NG 365
            + ++ ++ G G E +    V+ +R   +E+ + ++     +   S + SK+      N 
Sbjct: 308 MFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLMLTNY 367

Query: 366 QAAGGEV-EFCNFTSWCRFPVYEADFGWGKPSWVCSPSRP-YKNVVVLMTTRDGDGIEAW 425
                E  E    +SWC+ P+YEA FGW  P WV     P   N+ +L+ ++DG GIEA+
Sbjct: 368 SRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQGIEAF 427

Query: 426 VTLEEEDMASFERDSELISF 433
           VTL EE+M+SFE++ EL++F
Sbjct: 428 VTLPEENMSSFEQNPELLAF 437

BLAST of Lcy03g020020 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.2e-68
Identity = 167/436 (38.30%), Postives = 243/436 (55.73%), Query Frame = 0

Query: 4   ENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENP--GADRFSGS 63
           +N+ ++  E++ PSSPTP   R L LSLLD +S   YT  + FYAA      G      S
Sbjct: 3   KNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETS 62

Query: 64  SQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARVGCDLSEVVKNPDFKVLHR 123
            +LKKSLS+ L +FYP AG +   G  V+CND G  + EARV   LSE +K P  + L  
Sbjct: 63  LKLKKSLSKTLPIFYPLAGRII--GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLEL 122

Query: 124 LLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAIN 183
           L+P E  +     E     +L ++ N F CGG+ I +C++HK+ D  S  +F+R WA  +
Sbjct: 123 LIPVEAKSR----EAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESS 182

Query: 184 RGDSDQVSEPLFDGAKYFPP--KEIPG--LKRSKVVINDKIVTKRFVFDKANIAALRQEA 243
           RG    +  P F  ++ FP    E+P   + R + V     VTKRFVFD + I  LR +A
Sbjct: 183 RGLGITLI-PSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKA 242

Query: 244 AAGSLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSG 303
           +  +L K PTRVEAV+A  W+   +++ R      +       VNLRGK+D  L  N+ G
Sbjct: 243 SR-NLVKNPTRVEAVTALFWRCVTKVS-RLSSLTPRTSVLQILVNLRGKVD-SLCENTIG 302

Query: 304 NLWWFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAGVPSHMWSKMGKNGQ-- 363
           N+    I   E    +R   +V +IR A KEI S   + + ++   S ++  + + G+  
Sbjct: 303 NMLSLMILKNEEAAIERIQDVVDEIRRA-KEIFSLNCKEMSKSS--SRIFELLEEIGKVY 362

Query: 364 AAGGEVEFCNFTSWCRFPVYEADFGWGKPSWVCS-PSRPYKNVVVLMTTRDGDGIEAWVT 423
             G E++     SWC+  +Y+ADFGWGKP WV    +  +KN+++L+ T+DG+GIEAW+T
Sbjct: 363 GRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAWIT 422

Query: 424 LEEEDMASFERDSELI 431
           L EE M+ FE D EL+
Sbjct: 423 LTEEQMSLFECDQELL 425

BLAST of Lcy03g020020 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-67
Identity = 155/443 (34.99%), Postives = 243/443 (54.85%), Query Frame = 0

Query: 1   MAIENIHVIGDEVILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60
           M    +  I  E+I PSS TP  L+ L+LS+ D I P  YT    FY   +    ++   
Sbjct: 1   METMKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQ--S 60

Query: 61  SSQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDRGVEYSEARV-GCDLSEVVKNPDFKVL 120
           S +LK SL+E LT FYP AG +  +G ++DCND G  + +ARV    LS+ +++PDFK L
Sbjct: 61  SHKLKTSLAETLTKFYPLAGRI--KGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTL 120

Query: 121 HRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAA 180
            +LLP +   +  E      ++L V+   F CGG+AI LCITHK+ D  S + F++ WAA
Sbjct: 121 QQLLPLDVIDDPYEAATTWPLLL-VKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 180

Query: 181 INRGDS-DQVSEPLFDGAKYFPP-KEIPGLKRSKVVINDKIVTKRFVFDKANIAALRQEA 240
           + RG++ D V++P+F  A ++PP  E+      +       +TKRFVF  + +  LR +A
Sbjct: 181 MARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKA 240

Query: 241 AAGSLEKPPTRVEAVSAFLWKRFMEINPRSEQENEKIFAAVHAVNLRGKMDPPLPPNSSG 300
           A+      P RVE+V+A LWK F+     S      +   +   NLR K+   L  +  G
Sbjct: 241 ASEDFVGRPKRVESVTALLWKAFVAA-ASSNTNACDMKVLIQPSNLRPKIPSLLAESLIG 300

Query: 301 NLWWFAIAVAEGGIEKRHGYLVKQIRNAIKEIDSDLVRNLQQAG------VPSHMWSKMG 360
           N+ + ++ +   GI ++    +++    +++   DL   +++ G      + S + + M 
Sbjct: 301 NIMFSSVVL---GISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLML 360

Query: 361 KNGQAAGGEV-EFCNFTSWCRFPVYEADFGWGKPSWVCSPSRP-YKNVVVLMTTRDGDGI 420
            N      E  E    +SWC+ P+YEA FGWG P W+     P  +NV +L+ ++DG GI
Sbjct: 361 TNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQGI 420

Query: 421 EAWVTLEEEDMASFERDSELISF 433
           EA+VTL EE+M S E+++EL++F
Sbjct: 421 EAFVTLPEENMLSLEQNTELLAF 434