Lcy03g020000 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g020000 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.3e-67
Identity = 158/425 (37.18%), Postives = 238/425 (56.00%), Query Frame = 0

Query: 6   VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 65
           ++I+S E+I+PSSPTP +L   +LS +DQ+    + P I FY  Q   N+ D++ +   +
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNL-DRAQRSENL 64

Query: 66  RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLHRLLPL 125
           + SL+  LT+F  LAG +     VDCND GV F EA+V  ++S  + N  I  L++ LP 
Sbjct: 65  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLPF 124

Query: 126 VFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAADARGGN 185
                  ES    +I L V+   F CGG A+ VC+SHK+AD  S ATF+ +W A  +   
Sbjct: 125 QPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQEET 184

Query: 186 ESVLEPNFD-AVKLFPPRK---IPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAA 245
           + +++PNFD     FPP +    P F        E I  KR VF K  + ALK  A  A+
Sbjct: 185 D-IVQPNFDLGSHHFPPMESIPAPEF-----LPDENIVMKRFVFDKEKLEALK--AQLAS 244

Query: 246 GGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNV 305
             + VK  SRV  V + IW + I + R +        A   V+LR RM+PP P+++ GN+
Sbjct: 245 SATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGNI 304

Query: 306 SWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDV 365
           +  T+A +  E + D   LV  ++T++ +ID   VK+L+     LD   +  +       
Sbjct: 305 A--TMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYEAEPQE------- 364

Query: 366 HLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMA 425
            L+SF+SWCR   Y+ DFGWGKP  VC+ + P +N+V LMDT + DG+EAW+++ E++M+
Sbjct: 365 -LFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMS 410

Query: 426 IFEKD 427
           +   D
Sbjct: 425 MLSSD 410

BLAST of Lcy03g020000 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 248.8 bits (634), Expect = 1.2e-64
Identity = 165/428 (38.55%), Postives = 226/428 (52.80%), Query Frame = 0

Query: 6   VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 65
           +E +S E+I PSSPTP SL   ++S +DQ+    + P I FY      N+D      + +
Sbjct: 5   MEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS-QHL 64

Query: 66  RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDN-PEIGVLHRLLP 125
           + SL++ LT F  LAG +     VDCND GV F EA+V  ++S  + N  E+  L + LP
Sbjct: 65  KQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLP 124

Query: 126 LVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAADARGG 185
                      N  ++ L V+   F CGG A+ V LSHK+AD  S ATF+ AW A  RG 
Sbjct: 125 SAAYPGGKIEVN-EDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGE 184

Query: 186 NESVLEPNFD-AVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAGG 245
            E VL PNFD A + FPP  +           E +  KR VF K  I AL+  AS A   
Sbjct: 185 TEIVL-PNFDLAARHFPP--VDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSA--- 244

Query: 246 SVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVSW 305
           S  K  SRV  V ++IW  +I + R +      F  +  V+LR RM+PPLP  + GN++ 
Sbjct: 245 SEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGNIAT 304

Query: 306 FTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDVHL 365
              A    E + D P L+  +RT+++        K ED  N  +L+K +          L
Sbjct: 305 LLFAAVDAEWDKDFPDLIGPLRTSLE--------KTEDDHN-HELLKGMTCLYELEPQEL 364

Query: 366 WSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMAIF 425
            SFTSWCR   Y+ DFGWGKP   C+ + P +N  +LMDT  GDGVEAW+ + E++MA+ 
Sbjct: 365 LSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEMAML 413

Query: 426 EKDKELLS 432
               ELLS
Sbjct: 425 --PVELLS 413

BLAST of Lcy03g020000 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.1e-53
Identity = 149/444 (33.56%), Postives = 229/444 (51.58%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           M +  VEI+S+  IKPSSPTP SL    LS IDQ      T   FFY    + N   +S 
Sbjct: 1   MASKMVEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY----TYNPSHESS 60

Query: 61  KV-RRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVL 120
           K+   ++ SL++TL  +   AG + E  ++DCND GV+F E ++   ++ +L   +    
Sbjct: 61  KIGDLLKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFAT 120

Query: 121 HRLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAA 180
             +LP    +   +S      LL+VQ   F CGG+A+    SHK++DG S   F+K WA+
Sbjct: 121 DLVLPTRIIALHEDS------LLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS 180

Query: 181 DARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 240
                      P   A  +FPP            S ++   KR VF+   I  LK  A  
Sbjct: 181 STSLSTFHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE 240

Query: 241 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 300
                 ++ PSRV  V++F+       A     + K +  +  V+LR  +   LP+NS G
Sbjct: 241 YG----IQKPSRVEVVTAFL---CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIG 300

Query: 301 NVSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFV--KKLEDSENLLDLMKQVDKQLS 360
           N+  F    +   ++     L++K+R   Q++    +  +KL     + +L K +D QL+
Sbjct: 301 NIFSFYFCINDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLD-QLN 360

Query: 361 TGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDTSDGDGVEAWVNLK 420
              +  +  +SWCRFPVY+ DFGWGKP  V SP +P  +++++LMD+ +GDG+EA + ++
Sbjct: 361 ISSLDTYFCSSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITME 420

Query: 421 EEDMAIFEKDKELLSFC-SDSPIP 440
           E+ MA FEK++EL SF   DSP P
Sbjct: 421 EKKMAAFEKNEELRSFAYLDSPEP 423

BLAST of Lcy03g020000 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.9e-53
Identity = 158/480 (32.92%), Postives = 246/480 (51.25%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAP-PSYTPAIFFY------RPQKSE 60
           M + +VE+IS+E IKP++PTPS L    LSL+DQ  P   Y P I FY          S 
Sbjct: 4   MYSAAVEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSN 63

Query: 61  NVDDQSLKVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDN 120
           + DD  L    +++SL++TL  F  +AG M +   VDC+D G+ F++ K+  ++   +  
Sbjct: 64  HHDDLDL----LKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQ 123

Query: 121 PEIGVLHRLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATF 180
           P++  L +LLP    S    ++   E L++VQ   F CGG A+   +SHK+AD A+ +TF
Sbjct: 124 PDV-PLSQLLP----SEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTF 183

Query: 181 VKAWAADAR----GGN-----ESVLEPNFDAVKLFPP----------RKIPGFKRGIEAS 240
           +++WA+  +    GG+     +  L P+FD+  LFPP           +IP      +  
Sbjct: 184 IRSWASTTKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTE 243

Query: 241 KEKISTKRLVFAKSNIAALKE-LASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMK 300
            +K  +KR VF  + I +++E L          +  +RV  V+S IW  +        MK
Sbjct: 244 DDKTVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSV--------MK 303

Query: 301 AKLFGAL----YPVDLRGRMDPPLPENSFGN----VSWFTIATSPVEINA---------- 360
           +   G L    + V+LR +MDPPL + SFGN    VS F  AT+    NA          
Sbjct: 304 STPAGFLPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSS 363

Query: 361 -------DLPLLVAKVRTAIQEI--DSGFVKKLEDSENLLDLMKQVDKQLSTGDVHLWSF 420
                  +L   +A++R+ I ++  D G ++K+   +N         K+++  +V  +  
Sbjct: 364 ESQVVLHELHDFIAQMRSEIDKVKGDKGSLEKV--IQNFASGHDASIKKINDVEVINFWI 423

Query: 421 TSWCRFPVYEADFGWGKPTWV-CSPS-RPFQNVVVLMDTSDGDGVEAWVNLKEEDMAIFE 425
           +SWCR  +YE DFGWGKP WV   P+ +P +N   + DT  G+G+E W +  E+DMA FE
Sbjct: 424 SSWCRMGLYEIDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFE 464

BLAST of Lcy03g020000 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.0e-52
Identity = 145/447 (32.44%), Postives = 240/447 (53.69%), Query Frame = 0

Query: 6   VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFY-----RPQKSENVDDQSL 65
           V I  +  IKPSS TPS+L + +LS IDQ     Y P  FFY     R + S+N D+ S 
Sbjct: 10  VSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 66  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNP-----E 125
               ++ SL++TL  +   AG + +   VDCND+G +F   ++ C +S +LD+P     E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 126 IGVLHRLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVK 185
             VL + LP    +N  E  N    LLVVQ   F CGG+A++VC SHK+ DG S   F+ 
Sbjct: 130 SIVLPKDLP---WANNCEGGN----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLN 189

Query: 186 AWAADARGGNESVL--EPNFDAVKLFPPRKIPG-FKRGIEASKEKISTKRLVFAKSNIAA 245
            W++  R    + L   P F    +F  +K        I +   +   KRL+F    + A
Sbjct: 190 DWSSVTRDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDA 249

Query: 246 LKELASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPP 305
           L+   +  +G   VK P+R   VS+ ++ +    A +  + +KL   ++ +++R  + P 
Sbjct: 250 LRAKVAEESG---VKNPTRAEVVSALLF-KCATKASSSMLPSKL---VHFLNIRTMIKPR 309

Query: 306 LPENSFGNV-SWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVK-KLEDSENLLDLMK 365
           LP N+ GN+ S F+I  + ++ + +LP LV  +R   +E++  + K ++E +E +L++++
Sbjct: 310 LPRNAIGNLSSIFSIEATNMQ-DMELPTLVRNLR---KEVEVAYKKDQVEQNELILEVVE 369

Query: 366 QVDK-----QLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSD 425
            + +     +   G  ++++ ++ C++P Y  DFGWG+P  VC  + P +N   L D   
Sbjct: 370 SMREGKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKA 429

Query: 426 GDGVEAWVNLKEEDMAIFEKDKELLSF 433
           G GVEA V L ++ M+ FE+++ELL F
Sbjct: 430 GQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of Lcy03g020000 vs. ExPASy TrEMBL
Match: A0A6J1C1E9 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007495 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 9.5e-203
Identity = 359/434 (82.72%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENI V+IISR+IIKP+SPTP SL+YRELSLIDQMAPPSYTPAIFFYR QKSENV  QS+
Sbjct: 1   MENIGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSV 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KV ++RASLARTLTRFNHLAGTMTERF+VDCNDVGV+F EA+VDCEIS++L+ PEIGVLH
Sbjct: 61  KVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLH 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RLLPLVFTS      NGSE LL VQ  SF CGGVAVAVCLSHKLADGASAA FVK WAA 
Sbjct: 121 RLLPLVFTSR-----NGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARG   SVLEPNFDAVKL+PPRKIPGFKRGIEASKEKI T+RLVF K  IAALKELA+GA
Sbjct: 181 ARGDKHSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGSVVKPPSRVVAVS+FIWLRL+ LAR R +KAK+FGALYPVDLR RMDPPLPENSFGN
Sbjct: 241 GGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+ATSPVE N DLPLL  KVR+AIQEIDSGFVKKLEDSENLLDL+KQVDKQ+S+G+
Sbjct: 301 VSWFTMATSPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           + LWSFTSWCRFPVYEADFGWGKP+WVCSPSRPF+N VVLMD SDG GVEAWVNLKEEDM
Sbjct: 361 MQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDM 420

Query: 421 AIFEKDKELLSFCS 435
           AIF  D++LLSFCS
Sbjct: 421 AIFHHDQQLLSFCS 429

BLAST of Lcy03g020000 vs. ExPASy TrEMBL
Match: A0A6J1EG83 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434048 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 3.4e-200
Identity = 356/435 (81.84%), Postives = 386/435 (88.74%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF EAKVDC+IS LLDNPEIGVL 
Sbjct: 61  KVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RL+P       AES NGSE LLVVQ  SFICGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAY 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGSVV PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVVNPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+AT+PV +N DLPLLVAKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 421 AIFEKDKELLSFCSD 436
            IFEKD+ELLSFCS+
Sbjct: 421 TIFEKDEELLSFCSE 429

BLAST of Lcy03g020000 vs. ExPASy TrEMBL
Match: A0A5D3BIG7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00830 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.0e-192
Identity = 351/436 (80.50%), Postives = 383/436 (87.84%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           ME+  VEII+ +IIKPSS TP+   Y +LSLIDQMAPPSY PAIFFY P  S+N+     
Sbjct: 1   MEDFGVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNI----- 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
            V +IR SL+RTLTRF  LAGTM ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LH
Sbjct: 61  -VHQIRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILH 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RLLPLVFTS+ A+    S+ LL VQ   FICGGVAVAVCLSHKLADGASAA FVKAWAA+
Sbjct: 121 RLLPLVFTSHSAD----SKFLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAE 180

Query: 181 ARGG-NESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 240
           ARGG N S+LEPNFDAVKLFP RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G
Sbjct: 181 ARGGSNNSILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVG 240

Query: 241 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 300
             GGS+ KPPSRVVAVS+FIWLRL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFG
Sbjct: 241 TFGGSISKPPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFG 300

Query: 301 NVSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTG 360
           NVSWFTIATSPVEIN DLPLLVAKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G
Sbjct: 301 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSG 360

Query: 361 DVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEED 420
           +VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEED
Sbjct: 361 EVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEED 420

Query: 421 MAIFEKDKELLSFCSD 436
           MAIFEKD+ELLSFCSD
Sbjct: 421 MAIFEKDEELLSFCSD 423

BLAST of Lcy03g020000 vs. ExPASy TrEMBL
Match: A0A5A7USW8 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00800 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.0e-192
Identity = 351/436 (80.50%), Postives = 383/436 (87.84%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           ME+  VEII+ +IIKPSS TP+   Y +LSLIDQMAPPSY PAIFFY P  S+N+     
Sbjct: 1   MEDFGVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNI----- 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
            V +IR SL+RTLTRF  LAGTM ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LH
Sbjct: 61  -VHQIRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILH 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RLLPLVFTS+ A+    S+ LL VQ   FICGGVAVAVCLSHKLADGASAA FVKAWAA+
Sbjct: 121 RLLPLVFTSHSAD----SKFLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAE 180

Query: 181 ARGG-NESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 240
           ARGG N S+LEPNFDAVKLFP RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G
Sbjct: 181 ARGGCNNSILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVG 240

Query: 241 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 300
             GGS+ KPPSRVVAVS+FIWLRL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFG
Sbjct: 241 TFGGSISKPPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFG 300

Query: 301 NVSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTG 360
           NVSWFTIATSPVEIN DLPLLVAKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G
Sbjct: 301 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSG 360

Query: 361 DVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEED 420
           +VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEED
Sbjct: 361 EVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEED 420

Query: 421 MAIFEKDKELLSFCSD 436
           MAIFEKD+ELLSFCSD
Sbjct: 421 MAIFEKDEELLSFCSD 423

BLAST of Lcy03g020000 vs. ExPASy TrEMBL
Match: A0A1S3CLJ4 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103502350 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 6.5e-167
Identity = 300/354 (84.75%), Postives = 323/354 (91.24%), Query Frame = 0

Query: 83  MTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLHRLLPLVFTSNRAESTNGSEILL 142
           M ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LHRLLPLVFTS+ A+    S+ LL
Sbjct: 1   MPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILHRLLPLVFTSHSAD----SKFLL 60

Query: 143 VVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAADARGG-NESVLEPNFDAVKLFPP 202
            VQ   FICGGVAVAVCLSHKLADGASAA FVKAWAA+ARGG N S+LEPNFDAVKLFP 
Sbjct: 61  AVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAEARGGSNNSILEPNFDAVKLFPC 120

Query: 203 RKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAGGSVVKPPSRVVAVSSFIWL 262
           RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G  GGS+ KPPSRVVAVS+FIWL
Sbjct: 121 RKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVGTFGGSISKPPSRVVAVSAFIWL 180

Query: 263 RLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVSWFTIATSPVEINADLPLLV 322
           RL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFGNVSWFTIATSPVEIN DLPLLV
Sbjct: 181 RLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFGNVSWFTIATSPVEINEDLPLLV 240

Query: 323 AKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGW 382
           AKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G+VHLWSFTSWCRFPVYEADFGW
Sbjct: 241 AKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSGEVHLWSFTSWCRFPVYEADFGW 300

Query: 383 GKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMAIFEKDKELLSFCSD 436
           GKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEEDMAIFEKD+ELLSFCSD
Sbjct: 301 GKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEEDMAIFEKDEELLSFCSD 350

BLAST of Lcy03g020000 vs. NCBI nr
Match: XP_022135580.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 716.1 bits (1847), Expect = 2.0e-202
Identity = 359/434 (82.72%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENI V+IISR+IIKP+SPTP SL+YRELSLIDQMAPPSYTPAIFFYR QKSENV  QS+
Sbjct: 1   MENIGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSV 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KV ++RASLARTLTRFNHLAGTMTERF+VDCNDVGV+F EA+VDCEIS++L+ PEIGVLH
Sbjct: 61  KVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLH 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RLLPLVFTS      NGSE LL VQ  SF CGGVAVAVCLSHKLADGASAA FVK WAA 
Sbjct: 121 RLLPLVFTSR-----NGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARG   SVLEPNFDAVKL+PPRKIPGFKRGIEASKEKI T+RLVF K  IAALKELA+GA
Sbjct: 181 ARGDKHSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGSVVKPPSRVVAVS+FIWLRL+ LAR R +KAK+FGALYPVDLR RMDPPLPENSFGN
Sbjct: 241 GGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+ATSPVE N DLPLL  KVR+AIQEIDSGFVKKLEDSENLLDL+KQVDKQ+S+G+
Sbjct: 301 VSWFTMATSPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           + LWSFTSWCRFPVYEADFGWGKP+WVCSPSRPF+N VVLMD SDG GVEAWVNLKEEDM
Sbjct: 361 MQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDM 420

Query: 421 AIFEKDKELLSFCS 435
           AIF  D++LLSFCS
Sbjct: 421 AIFHHDQQLLSFCS 429

BLAST of Lcy03g020000 vs. NCBI nr
Match: XP_022927112.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 707.6 bits (1825), Expect = 7.0e-200
Identity = 356/435 (81.84%), Postives = 386/435 (88.74%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF EAKVDC+IS LLDNPEIGVL 
Sbjct: 61  KVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RL+P       AES NGSE LLVVQ  SFICGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAY 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGSVV PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVVNPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+AT+PV +N DLPLLVAKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 421 AIFEKDKELLSFCSD 436
            IFEKD+ELLSFCS+
Sbjct: 421 TIFEKDEELLSFCSE 429

BLAST of Lcy03g020000 vs. NCBI nr
Match: KAG7021414.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-198
Identity = 354/434 (81.57%), Postives = 384/434 (88.48%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 346 MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 405

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF EAKVDC+IS LLDNPEIGVL 
Sbjct: 406 KVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 465

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RL+P       AES NGSE LLVVQ  SFICGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 466 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLADFVKAWAAY 525

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 526 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 585

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGS+V PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 586 GGGSLVNPPSRVVAVSTFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 645

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+AT+PV +N DLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 646 VSWFTVATTPVAMNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 705

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 706 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 765

Query: 421 AIFEKDKELLSFCS 435
            IFEKD+ELLSFCS
Sbjct: 766 TIFEKDEELLSFCS 773

BLAST of Lcy03g020000 vs. NCBI nr
Match: XP_023529997.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.5 bits (1804), Expect = 1.9e-197
Identity = 353/434 (81.34%), Postives = 381/434 (87.79%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MEN SVEI+S ++IKPSSPTP SL YRELSLIDQMA PS TPAIFFYRP   +N+  +SL
Sbjct: 1   MENTSVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPSLTPAIFFYRPSNPQNLAHRSL 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           K+R+IRASLARTLT F HLAGTM ERF+V CNDVGVQF EAKVDC+IS LLDNPEIGVL 
Sbjct: 61  KLRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RL+P       AES NGSE LLVVQ  SFICGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLAAFVKAWAAY 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGSV  PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVANPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+AT+PV  N DLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVATNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 421 AIFEKDKELLSFCS 435
           AIFEKD+ELLSFCS
Sbjct: 421 AIFEKDEELLSFCS 428

BLAST of Lcy03g020000 vs. NCBI nr
Match: KAG6587430.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 699.5 bits (1804), Expect = 1.9e-197
Identity = 352/434 (81.11%), Postives = 382/434 (88.02%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           MENISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 120
           KVR+IR SLARTLT F HLAGTM ERF+V CNDVGVQF EAKVDC+IS LLDNPEIGVL 
Sbjct: 61  KVRQIRTSLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 121 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 180
           RL+P       AES NGSE LLVVQ  SFICGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLADFVKAWAAY 180

Query: 181 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 240
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 241 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 300
            GGS+V PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSLVNPPSRVVAVSTFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 301 VSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 360
           VSWFT+AT+PV +N DLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 361 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 420
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEED 
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDK 420

Query: 421 AIFEKDKELLSFCS 435
            IFEKD+ELLSFCS
Sbjct: 421 TIFEKDEELLSFCS 428

BLAST of Lcy03g020000 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 302.8 bits (774), Expect = 4.8e-82
Identity = 172/443 (38.83%), Postives = 258/443 (58.24%), Query Frame = 0

Query: 4   ISVEIISREIIKPSSPTPSSL----MYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQS 63
           + + + S+E++KPS   P +L     +  LS +DQ+APP + P +FFY      N+ D+ 
Sbjct: 3   LEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFY--HNKTNLSDKE 62

Query: 64  LKVRRIRASLARTLTRFNHLAGTM-TERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGV 123
            +   I++SL+  L  +  LAG +      V CNDVGV F EAK DC +S +L+NP    
Sbjct: 63  -RSDHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNE 122

Query: 124 LHRLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWA 183
           L++L P  F          S++ L VQ   F CGG+A+ + LSHKL D  S   FV +WA
Sbjct: 123 LNKLHPFEF-------HEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWA 182

Query: 184 ADARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELAS 243
           A ARG  + ++ P+FD  K+FPP  I         +KE I T+R VF +S++ +L+E  S
Sbjct: 183 AFARGQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFS 242

Query: 244 GAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSF 303
               G+     +RV  +S FIW R +A         K++  ++PV+LR + DP +P+N F
Sbjct: 243 ----GNKKIRATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMF 302

Query: 304 GNVSWFTIATSPVEIN---ADLPLLVAKVRTAIQEIDSGFVKKL-EDSENLLDLMKQVDK 363
           GN+  F++    + IN    +   LV ++R  I++ID+ +VKKL ED+   L+ + +   
Sbjct: 303 GNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQAS 362

Query: 364 QLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVN 423
               G++  +SFTS C+FPVYEADFGWGKP WV S    ++N+V  +DT +GDG+EAW+N
Sbjct: 363 GFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWIN 422

Query: 424 LKEEDMAIFEKDKELLSFCSDSP 438
           L + DM+ FE D+ELL + S +P
Sbjct: 423 LDQNDMSRFEADEELLRYVSSNP 428

BLAST of Lcy03g020000 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 283.5 bits (724), Expect = 3.0e-76
Identity = 170/442 (38.46%), Postives = 246/442 (55.66%), Query Frame = 0

Query: 6   VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 65
           V+++SR+IIKPSSPTP+ L   +LSL++Q+ P  + P +FFY    S    +Q   ++ +
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQ---LQML 62

Query: 66  RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLHRLLPL 125
           + SL+ TLT F  LAG +     +DCND G  F EA+V+  +S LL  P    L +L+P 
Sbjct: 63  KKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIP- 122

Query: 126 VFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAA-DARGG 185
                  +S      LL+ Q   F CG +++ VC+SHKLAD  S   F+K+WAA  +RG 
Sbjct: 123 ----TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 182

Query: 186 NESVLEPNFDAVKLFPPRKI----PGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 245
            +++  P FD VK+FPP       P      E    +  +KR +F  S+I AL+  AS  
Sbjct: 183 IKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 242

Query: 246 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 305
                V  P+RV AVS+ IW   +   RT    +K       V LR R+ PP  +NS GN
Sbjct: 243 E----VNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGN 302

Query: 306 VSWFTIATSPVEIN-ADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLM----KQVDKQ 365
           +  +  A +   IN   L  LV+K+R A Q      + KL  + N  +++    K+    
Sbjct: 303 LVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDM 362

Query: 366 LSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNL 425
           +++GD   + F+S CRF +YE DFGWGKP WV  PS   +N+V L+DT +  G+EAWVNL
Sbjct: 363 IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNL 422

Query: 426 KEEDMAIFEKDKELLSFCSDSP 438
            E++M +FE+D+ELL F S +P
Sbjct: 423 NEQEMNLFEQDRELLQFASLNP 432

BLAST of Lcy03g020000 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.7e-64
Identity = 158/443 (35.67%), Postives = 248/443 (55.98%), Query Frame = 0

Query: 5   SVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYR--PQK--SENVDDQSL 64
           +VEI+SREI+KPSSPTP       LSL+D ++ P YT A+ FY   PQ     + ++ SL
Sbjct: 4   NVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSL 63

Query: 65  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKVDCEISMLLDNPEIGVLH 124
           K+++   SL++TL  F  LAG +    FV+CND G  F EA+VD  +S  L  P    L 
Sbjct: 64  KLKK---SLSKTLPIFYPLAGRIIGS-FVECNDEGAVFIEARVDHLLSEFLKCPVPESLE 123

Query: 125 RLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAAD 184
            L+P+   S  A +      +L++Q   F CGG+ + +C+SHK+ D  S A F++ WA  
Sbjct: 124 LLIPVEAKSREAVTWP----VLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAES 183

Query: 185 ARGGNESVLEPNFDAVKLFPP--RKIPG--FKRGIEASKEKISTKRLVFAKSNIAALKEL 244
           +RG   +++ P+F A ++FP    ++P     R  E  +    TKR VF  S I  L+  
Sbjct: 184 SRGLGITLI-PSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 243

Query: 245 ASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPEN 304
           AS     ++VK P+RV AV++  W  +  ++R   +  +       V+LRG++D  L EN
Sbjct: 244 AS----RNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVD-SLCEN 303

Query: 305 SFGNVSWFTIATSPVEINADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQL 364
           + GN+    I  +       +  +V ++R A +       +  + S  + +L++++ K  
Sbjct: 304 TIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEIGKVY 363

Query: 365 STG-DVHLWSFTSWCRFPVYEADFGWGKPTWVCS-PSRPFQNVVVLMDTSDGDGVEAWVN 424
             G ++ LW   SWC+  +Y+ADFGWGKP WV    +  F+N+++L+DT DG+G+EAW+ 
Sbjct: 364 GRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAWIT 423

Query: 425 LKEEDMAIFEKDKELLSFCSDSP 438
           L EE M++FE D+ELL   S +P
Sbjct: 424 LTEEQMSLFECDQELLESASLNP 432

BLAST of Lcy03g020000 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 239.2 bits (609), Expect = 6.6e-63
Identity = 157/448 (35.04%), Postives = 246/448 (54.91%), Query Frame = 0

Query: 1   MENISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 60
           ME + VE I++EIIKPSS TP+ L   +LS+ D + PP YT A  FY      + +  S 
Sbjct: 1   METMKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSS- 60

Query: 61  KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKV-DCEISMLLDNPEIGVL 120
              +++ SLA TLT+F  LAG + +   +DCND G  F +A+V +  +S  L +P+   L
Sbjct: 61  --HKLKTSLAETLTKFYPLAGRI-KGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTL 120

Query: 121 HRLLPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAA 180
            +LLPL    +  E+   +  LL+V+   F CGG+A+ +C++HK+AD  S +TF+++WAA
Sbjct: 121 QQLLPLDVIDDPYEAAT-TWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 180

Query: 181 DARG-GNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKIS--TKRLVFAKSNIAALKEL 240
            ARG   + V +P F A   +PP     FK  ++ +  KIS  TKR VF+ S    L+EL
Sbjct: 181 MARGEAGDIVADPVFAAANFYPPAN-ELFKFPVDENANKISQITKRFVFSASK---LEEL 240

Query: 241 ASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPEN 300
            + AA    V  P RV +V++ +W   +A A +      +   + P +LR ++   L E+
Sbjct: 241 RTKAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIPSLLAES 300

Query: 301 SFGNVSWFTIA---TSPVEINADLPLL-VAKVRTAIQEI--DSGFVKKLEDSENLLDLMK 360
             GN+ + ++    +   EI  +  +  + K    +Q +  + G          L +LM 
Sbjct: 301 LIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLML 360

Query: 361 QVDKQLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDTSDGDGV 420
               +LS      ++ +SWC+ P+YEA FGWG P W+     P  +NV +L+D+ DG G+
Sbjct: 361 TNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQGI 420

Query: 421 EAWVNLKEEDMAIFEKDKELLSFCSDSP 438
           EA+V L EE+M   E++ ELL+F S +P
Sbjct: 421 EAFVTLPEENMLSLEQNTELLAFASVNP 439

BLAST of Lcy03g020000 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-62
Identity = 158/450 (35.11%), Postives = 244/450 (54.22%), Query Frame = 0

Query: 4   ISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVR 63
           + VE IS+EIIKPSSPTP++L   +LS+ D + PP YT A  FY      ++  Q     
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY---TKNDLISQEHTSH 65

Query: 64  RIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFFEAKV-DCEISMLLDNPEIGVLHRL 123
           +++ SL+ TLT+F  LAG +T    VDC D G  F +A+V +C ++  L  P+   L +L
Sbjct: 66  KLKTSLSETLTKFYPLAGRIT-GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 125

Query: 124 LPLVFTSNRAESTNGSEILLVVQCCSFICGGVAVAVCLSHKLADGASAATFVKAWAADAR 183
           LPL    N   +   +  LL+V+   F CGG+A+ +C++HK+AD AS +TF+++WAA AR
Sbjct: 126 LPLDVVDNPYVAA-ATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 185

Query: 184 GGNESVL--EPNFDAVKLFPPR----KIPGFKRGIEASKEKISTKRLVFAKSNIAALKEL 243
           G N++     P F     +PP     K+P  +   +A K    TKR VF  S +   ++L
Sbjct: 186 GENDAAAMESPVFAGANFYPPANEAFKLPADE---QAGKRSSITKRFVFEASKV---EDL 245

Query: 244 ASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPEN 303
            + AA    V  P+RV +V++ IW   +A ++T     K+   L   +LR ++   L E+
Sbjct: 246 RTKAASEETVDQPTRVESVTALIWKCFVASSKTTTCDHKVLVQL--ANLRSKIPSLLQES 305

Query: 304 SFGNVSWFTIATS-----PVEIN---ADLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDL 363
           S GN+ + ++  S      V+I     DL     ++ T I +              L +L
Sbjct: 306 SIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANL 365

Query: 364 MKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDTSDGD 423
           M     +LS      ++ +SWC+ P+YEA FGW  P WV     P   N+ +L+D+ DG 
Sbjct: 366 MLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQ 425

Query: 424 GVEAWVNLKEEDMAIFEKDKELLSFCSDSP 438
           G+EA+V L EE+M+ FE++ ELL+F + +P
Sbjct: 426 GIEAFVTLPEENMSSFEQNPELLAFATMNP 442