Lcy03g019280 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g019280
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReplication termination factor 2
LocationChr03: 52840209 .. 52841905 (+)
RNA-Seq ExpressionLcy03g019280
SyntenyLcy03g019280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAACTGCCTGGATTTTTCTAATCTCTTTTGGAAAAGGAATTTCTCCCGGCTCCTTGTTCATTCCTCGAGTTGCAAAATCGTCGTCGTTCTGATTGTAATCGAAACCCTAGCTAAAGAATTTCGTCGAGAATCATCGTCGTTCTGATCGTGATCGCGAAACCCTAGCCACGGAATTTCGTCATCGTCTTCGATTCTCGAAACCCTAACCAATGCATCCGAAAAATCAACACCGTCTTCAGATCTTCGTTCACTCTCTCGACCTCCAAATTCAATCCGAAATCGTAAACCTAGCACGAAATTCGTCCCAAACCCTTGATGATCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCTTCACGGTTTGCGTCATCTTTCTACTTCACTCTGAATGGGAAACCCCTCCATGATTCCACCACAATTTCCAGTTCTCTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGTGATGGCGGTGCTACAGGGGCTGAGTCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAACCCGATAAGGTCGACCCCAACGAGCAGAGGCTGTCCAAATGGTTGAACTGCGCTCTTTCTAACGAGCCTTTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAATATCTTCAATAAGGAGTCGCTCGTGCACGCTTTGCTGGAGAAGAAGCTGCCAAAGGGATTCGGGCATATCAAGGGTCTCAAGGATATGATCAAGATAAATCTTTCGATGATTCCCGGTACAGAATCGCGTGGGAATGCTATTTCAGAGCCGCGGTTTCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGATCTTGTGGACACGTGCTTAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTGTCTGTCATGCTGAGTTTACGGTCAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAGGAGAAATCGAAATCTAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGGATGAATGGAGATGTGAATGTGGATTTGGCATCATCCCGTTTGTCCGGTAAAAAGCATGGTATTGAAGTTAAGGCTTTGGAGAAGGTTTCAGCTAAGCCTGAGAAGCATGAGCGGCCAGATAGTGGAGTTCAGGCAAAGCTTGCAGCAAGTAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTGCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGATTGGTGTTAAGAACTGCTTAGATCGGTTTATGGTGATTCTCCTTATACTGTTTAGTGTTTGTATTCAAAACTTCATTTATAGTGCCCTTTTTTGACTTATTGATTCATGTCTCATCGTTTCTAATTAATGTACGATATAAGTTTACAGTCATCAACTGCTCTTACTCTGTTGAACTGTTTTAACATTTTTATATCAGTCAATATTGTTGAAGGTCTTGCGCCAATCAATTCACGATCCTCTAGTAGTACAACATTTGAGTTGATGATTGAGGTATCGAAAATGAAACCCCTGATGGAGTGCTTAATTTGCATATTTTGACTTC

mRNA sequence

AAACAAACTGCCTGGATTTTTCTAATCTCTTTTGGAAAAGGAATTTCTCCCGGCTCCTTGTTCATTCCTCGAGTTGCAAAATCGTCGTCGTTCTGATTGTAATCGAAACCCTAGCTAAAGAATTTCGTCGAGAATCATCGTCGTTCTGATCGTGATCGCGAAACCCTAGCCACGGAATTTCGTCATCGTCTTCGATTCTCGAAACCCTAACCAATGCATCCGAAAAATCAACACCGTCTTCAGATCTTCGTTCACTCTCTCGACCTCCAAATTCAATCCGAAATCGTAAACCTAGCACGAAATTCGTCCCAAACCCTTGATGATCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCTTCACGGTTTGCGTCATCTTTCTACTTCACTCTGAATGGGAAACCCCTCCATGATTCCACCACAATTTCCAGTTCTCTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGTGATGGCGGTGCTACAGGGGCTGAGTCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAACCCGATAAGGTCGACCCCAACGAGCAGAGGCTGTCCAAATGGTTGAACTGCGCTCTTTCTAACGAGCCTTTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAATATCTTCAATAAGGAGTCGCTCGTGCACGCTTTGCTGGAGAAGAAGCTGCCAAAGGGATTCGGGCATATCAAGGGTCTCAAGGATATGATCAAGATAAATCTTTCGATGATTCCCGGTACAGAATCGCGTGGGAATGCTATTTCAGAGCCGCGGTTTCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGATCTTGTGGACACGTGCTTAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTGTCTGTCATGCTGAGTTTACGGTCAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAGGAGAAATCGAAATCTAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGGATGAATGGAGATGTGAATGTGGATTTGGCATCATCCCGTTTGTCCGGTAAAAAGCATGGTATTGAAGTTAAGGCTTTGGAGAAGGTTTCAGCTAAGCCTGAGAAGCATGAGCGGCCAGATAGTGGAGTTCAGGCAAAGCTTGCAGCAAGTAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTGCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGATTGGTGTTAAGAACTGCTTAGATCGGTTTATGGTGATTCTCCTTATACTGTTTAGTGTTTGTATTCAAAACTTCATTTATAGTGCCCTTTTTTGACTTATTGATTCATGTCTCATCGTTTCTAATTAATGTACGATATAAGTTTACAGTCATCAACTGCTCTTACTCTGTTGAACTGTTTTAACATTTTTATATCAGTCAATATTGTTGAAGGTCTTGCGCCAATCAATTCACGATCCTCTAGTAGTACAACATTTGAGTTGATGATTGAGGTATCGAAAATGAAACCCCTGATGGAGTGCTTAATTTGCATATTTTGACTTC

Coding sequence (CDS)

ATGCATCCGAAAAATCAACACCGTCTTCAGATCTTCGTTCACTCTCTCGACCTCCAAATTCAATCCGAAATCGTAAACCTAGCACGAAATTCGTCCCAAACCCTTGATGATCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCTTCACGGTTTGCGTCATCTTTCTACTTCACTCTGAATGGGAAACCCCTCCATGATTCCACCACAATTTCCAGTTCTCTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGTGATGGCGGTGCTACAGGGGCTGAGTCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAACCCGATAAGGTCGACCCCAACGAGCAGAGGCTGTCCAAATGGTTGAACTGCGCTCTTTCTAACGAGCCTTTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAATATCTTCAATAAGGAGTCGCTCGTGCACGCTTTGCTGGAGAAGAAGCTGCCAAAGGGATTCGGGCATATCAAGGGTCTCAAGGATATGATCAAGATAAATCTTTCGATGATTCCCGGTACAGAATCGCGTGGGAATGCTATTTCAGAGCCGCGGTTTCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGATCTTGTGGACACGTGCTTAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTGTCTGTCATGCTGAGTTTACGGTCAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAGGAGAAATCGAAATCTAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGGATGAATGGAGATGTGAATGTGGATTTGGCATCATCCCGTTTGTCCGGTAAAAAGCATGGTATTGAAGTTAAGGCTTTGGAGAAGGTTTCAGCTAAGCCTGAGAAGCATGAGCGGCCAGATAGTGGAGTTCAGGCAAAGCTTGCAGCAAGTAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTGCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGA

Protein sequence

MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLNGKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKINLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVYASIFTSSKKSDFKETFTCRSLPLGRN
Homology
BLAST of Lcy03g019280 vs. ExPASy Swiss-Prot
Match: Q3T1J8 (Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-31
Identity = 97/271 (35.79%), Postives = 145/271 (53.51%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEVLRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HIKGLKDMIKINLSMIPGTE-SRGNA-------ISEPRFQCPITGLEFNGKYKFFALR 231
             HI+ +K++ ++ LS  P  E  +GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  TSHIRSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 SCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSK-EKK 291
            CG V S +ALKE+K+  C  C A F   D  V+NG++E+V  ++ RMEE + ++K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKRRMEERRLRAKLEKK 198

Query: 292 TKKVRNGEVGMNGDVNVDLA--SSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLA 351
           TKK +  E      ++ D A  S   +GK    +    EK S+   +    +     K+ 
Sbjct: 199 TKKPKTAESASKLGISQDSAGPSKAKAGKSEEADPDPREKKSSLAPRGTASNGSASGKVG 258

Query: 352 ASN-GAVKRFKAADMAPANATKEVYASIFTS 367
               GA+KR   AD    +   E Y SIFTS
Sbjct: 259 KPPCGALKR-SIAD----SEESETYKSIFTS 284

BLAST of Lcy03g019280 vs. ExPASy Swiss-Prot
Match: Q99K95 (Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.5e-30
Identity = 98/272 (36.03%), Postives = 143/272 (52.57%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HIKGLKDMIKINLSMIPGTE-SRGNA-------ISEPRFQCPITGLEFNGKYKFFALR 231
             HI+ +K++ ++ LS  P  E  +GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIRSIKNVTELRLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 SCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSK-EKK 291
            CG V S +ALKE+K+  C  C A F   D  V+NG++E+V  +++RMEE + ++K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKKRMEERRLRAKLEKK 198

Query: 292 TKKVRNG-EVGMNGDVNVDLA--SSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKL 351
           TKK +   E         D A  S   SGK    +    EK S    +    +     K+
Sbjct: 199 TKKPKTATECASKPGTTQDSAGPSKVKSGKPEEADPDPREKKSTPAPRGAATNGSASGKV 258

Query: 352 AASN-GAVKRFKAADMAPANATKEVYASIFTS 367
                GA+KR   AD    +   E Y SIFTS
Sbjct: 259 GKPPCGALKR-SIAD----SEESETYKSIFTS 285

BLAST of Lcy03g019280 vs. ExPASy Swiss-Prot
Match: Q5R9P9 (Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.9e-30
Identity = 99/285 (34.74%), Postives = 144/285 (50.53%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HIKGLKDMIKINLSMIPGTE-SRGNA-------ISEPRFQCPITGLEFNGKYKFFALR 231
             HIK +K++ ++ LS  P  E  +GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 SCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSK-EKK 291
            CG V S +ALKE+K+  C  C A F   D  V+NG++E+V  ++ RMEE + ++K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 TKKVRNGEVGMNGDVNVDLASSRLSGKKHGIEVKALEKV-SAKPEKHERPDSGVQAKLAA 351
           TKK +  E     DV+               E     KV + KPE+        +  LA 
Sbjct: 199 TKKPKAAESVSKPDVSE--------------EAPGPSKVKTGKPEEASLDSREKKTNLAP 258

Query: 352 SNGAVKRFKA--ADMAPANATKEVYASIFTSSKKSDFKETFTCRS 381
            + A     +  A   P  ATK    SI  S +   +K  FT  S
Sbjct: 259 KSTATNESSSGKAGKPPCGATKR---SIADSEESEAYKSLFTTHS 286

BLAST of Lcy03g019280 vs. ExPASy Swiss-Prot
Match: Q9BY42 (Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 98/285 (34.39%), Postives = 145/285 (50.88%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HIKGLKDMIKINLSMIPGTE-SRGNA-------ISEPRFQCPITGLEFNGKYKFFALR 231
             HIK +K++ ++ LS  P  E  +GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 SCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSK-EKK 291
            CG V S +ALKE+K+  C  C A F   D  ++NG++E+V  ++ RMEE + ++K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIMLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 TKKVRNGEVGMNGDVNVDLASSRLSGKKHGIEVKALEKV-SAKPEKHERPDSGVQAKLAA 351
           TKK +  E     DV+               E     KV + KPE+        +  LA 
Sbjct: 199 TKKPKAAESVSKPDVSE--------------EAPGPSKVKTGKPEEASLDSREKKTNLAP 258

Query: 352 SNGAVKRFKA--ADMAPANATKEVYASIFTSSKKSDFKETFTCRS 381
            + A+    +  A   P  ATK    SI  S +   +K  FT  S
Sbjct: 259 KSTAMNESSSGKAGKPPCGATKR---SIADSEESEAYKSLFTTHS 286

BLAST of Lcy03g019280 vs. ExPASy Swiss-Prot
Match: Q4R594 (Replication termination factor 2 OS=Macaca fascicularis OX=9541 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.7e-29
Identity = 96/275 (34.91%), Postives = 146/275 (53.09%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HIKGLKDMIKINLSMIPGTE-SRGNA-------ISEPRFQCPITGLEFNGKYKFFALR 231
             HIK +K++ ++ LS  P  E  +GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 SCGHVLSAKALKEVKSSSCLVCHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSK-EKK 291
            CG V S +ALKE+K+  C  C A F   D  V+NG+ E+V  ++ RMEE + ++K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTNEDVDVLKARMEERRLRAKLEKK 198

Query: 292 TKKVRNGEVGMNGDVNVDL--ASSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLA 351
           TKK +  E     DV+ +    S   +GK     + + EK +    K    +     K  
Sbjct: 199 TKKPKAAESVSKPDVSEEAPGPSKVKTGKPEETSLDSREKKNNLAPKSAATNESFSGKAG 258

Query: 352 AS-NGAVKRFKAADMAPANATKEVYASIFTSSKKS 371
               GA KR   AD   + A K ++ +  +S+K+S
Sbjct: 259 KPLCGATKR-SIADSEESEAYKSLFTT-HSSAKRS 291

BLAST of Lcy03g019280 vs. ExPASy TrEMBL
Match: A0A6J1F067 (Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.8e-193
Identity = 349/386 (90.41%), Postives = 364/386 (94.30%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FVHS DLQIQS+IVNLA+ S+Q+L DLK SL+  T ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSM+PGTESRGNAISE RFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDKFVINGSEEEV  MRERMEEEK+KSKEKKTKKVR GEVGMNGDV+VDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAKPEKHERPDSGVQ K+AASNGAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. ExPASy TrEMBL
Match: A0A6J1KUM3 (Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 4.1e-191
Identity = 347/386 (89.90%), Postives = 362/386 (93.78%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FV S DLQIQS+IVNLA+ S+Q+L DLK SLL    ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVRSPDLQIQSKIVNLAQTSAQSLADLKLSLLLNAPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSMIPGTESRGN ISE RFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNEISEARFQCPITGLEFNGKYKFFALRACGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDKFVINGSEEEV  MRERMEEEK+K+KEKKTKKVR GEVGMNGDV+VDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKAKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL K+SAKPEKHERPDSGVQ K+AASNGAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKISAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. ExPASy TrEMBL
Match: A0A5A7SWS4 (Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00470 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 345/388 (88.92%), Postives = 364/388 (93.81%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           MHPKNQHRLQIF+HS DLQIQS+IV+L + S++TL+DLKFSLL +TLASR ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DSTTI  SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTTI--SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGN+FNKESLV ALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           N SMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEE--KSKSKEKKTKKVRNGEVGMNGDVNVDLA 300
           CH EFT RDKFVINGSEEEV EMRERMEEE  KSKSKEKKTKKVRNGEVGMNGDV +DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIE++ LEKVSAKPE+HER D G Q K+AASNGAVKRFKAADM PANATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 387
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. ExPASy TrEMBL
Match: A0A1S3B9F3 (Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 345/388 (88.92%), Postives = 364/388 (93.81%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           MHPKNQHRLQIF+HS DLQIQS+IV+L + S++TL+DLKFSLL +TLASR ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DSTTI  SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTTI--SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGN+FNKESLV ALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           N SMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEE--KSKSKEKKTKKVRNGEVGMNGDVNVDLA 300
           CH EFT RDKFVINGSEEEV EMRERMEEE  KSKSKEKKTKKVRNGEVGMNGDV +DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIE++ LEKVSAKPE+HER D G Q K+AASNGAVKRFKAADM PANATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 387
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. ExPASy TrEMBL
Match: A0A6J1C136 (Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.1e-187
Identity = 338/386 (87.56%), Postives = 362/386 (93.78%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           MHPKNQ RLQIFVHS DLQIQS+I+NLAR S+QTL+DLKFSLLP+TLA R ASSFYFTLN
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DST ++SSLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKESLV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS PRFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDK VINGSEEEVA +RERMEEEKSKSKEK+ KKV+NGEVGMNGDV+VDLASS
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIEVKA EKVSAK E++ERPD G+Q K+AA+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. NCBI nr
Match: XP_038879920.1 (replication termination factor 2 [Benincasa hispida])

HSP 1 Score: 694.9 bits (1792), Expect = 4.0e-196
Identity = 352/388 (90.72%), Postives = 373/388 (96.13%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           MHPKN+HRLQIF+HSLDLQIQS+IVNL ++ +QTL+DLKFSLLP+TLASR ASSFYFTLN
Sbjct: 1   MHPKNEHRLQIFLHSLDLQIQSQIVNLPQSPAQTLEDLKFSLLPETLASRVASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DSTTI SSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLPDSTTIPSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKESLVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLS+IPGTESRGNAISEPRFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSVIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSK--SKEKKTKKVRNGEVGMNGDVNVDLA 300
           CHAEFT RDKFVINGSEEEV EMRERMEEEKSK  SKEKKTKKVRN E G+NGD++VD A
Sbjct: 241 CHAEFTNRDKFVINGSEEEVEEMRERMEEEKSKSRSKEKKTKKVRNEEGGINGDMSVDFA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIEVKALEKVSAKPE+HERPD GVQ K+AASNGAVKRFKAADM PANATKE
Sbjct: 301 ASRLSGKKHGIEVKALEKVSAKPERHERPDGGVQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 387
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 388

BLAST of Lcy03g019280 vs. NCBI nr
Match: XP_022933589.1 (protein RTF2 homolog [Cucurbita moschata])

HSP 1 Score: 682.6 bits (1760), Expect = 2.0e-192
Identity = 349/386 (90.41%), Postives = 364/386 (94.30%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FVHS DLQIQS+IVNLA+ S+Q+L DLK SL+  T ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSM+PGTESRGNAISE RFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDKFVINGSEEEV  MRERMEEEK+KSKEKKTKKVR GEVGMNGDV+VDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAKPEKHERPDSGVQ K+AASNGAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. NCBI nr
Match: KAG6587836.1 (Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 678.3 bits (1749), Expect = 3.8e-191
Identity = 348/386 (90.16%), Postives = 362/386 (93.78%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FVHS DLQIQS+IVNLA+ S+Q+L DLK SL+  T ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSMIPGTESRGNAISE RFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDKFVINGSEEEV  MRERMEEEK+KSKEKKTKKVR GEVGMNGDV+VDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAK EKHERPDSGVQ K+AASNG VKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. NCBI nr
Match: KAG7035670.1 (Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 677.9 bits (1748), Expect = 5.0e-191
Identity = 347/386 (89.90%), Postives = 362/386 (93.78%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FVHS DLQIQS+IVNLA+ S+Q+L DLK SL+  T ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSMIPGTESRGNAISE RFQCPITGLEFNGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CHAEF  RDKFVINGSEEEV  MRERMEEEK+KSKEKKTKKVR GEVGMNGDV+VDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAK EKHERPDSGVQ K+AASNG VKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. NCBI nr
Match: XP_023531451.1 (protein RTF2 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.6 bits (1747), Expect = 6.5e-191
Identity = 348/386 (90.16%), Postives = 361/386 (93.52%), Query Frame = 0

Query: 1   MHPKNQHRLQIFVHSLDLQIQSEIVNLARNSSQTLDDLKFSLLPKTLASRFASSFYFTLN 60
           M+PKNQHRLQ FVHS DLQIQS IVNLA+ S+Q+L DLK SLL KT ASR ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSTIVNLAQTSAQSLADLKLSLLLKTPASRLASSFYFTLN 60

Query: 61  GKPLHDSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DS TISSSL+PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180
           EQRLSKWLNC+LSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI
Sbjct: 121 EQRLSKWLNCSLSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLV 240
           NLSMIPGTESRGNAISE RFQCPITGLE NGKYKFFALR+CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLESNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTVRDKFVINGSEEEVAEMRERMEEEKSKSKEKKTKKVRNGEVGMNGDVNVDLASS 300
           CH EF  RDKFVINGSEEEV  MRERMEEEK+KSKEKKTKKVR GEVGMNGDV+VDLA S
Sbjct: 241 CHTEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLAPS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAKPEKHERPDSGVQ K+AASNG VKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 387
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Lcy03g019280 vs. TAIR 10
Match: AT5G58020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 (InterPro:IPR006735); Has 385 Blast hits to 385 proteins in 189 species: Archae - 0; Bacteria - 0; Metazoa - 153; Fungi - 117; Plants - 50; Viruses - 0; Other Eukaryotes - 65 (source: NCBI BLink). )

HSP 1 Score: 385.6 bits (989), Expect = 4.8e-107
Identity = 225/385 (58.44%), Postives = 264/385 (68.57%), Query Frame = 0

Query: 8   RLQIFVHSLDLQIQSEIVNLARNSSQTLDDLK--FSLLPKTLASRFASSFYFTLNGKPLH 67
           R QIFV S D Q   ++V L  + +Q+L  L    SLL  +    F S+   TL+GK L+
Sbjct: 4   RRQIFVKSPDCQ---KVVALQLDPAQSLLTLSGITSLLESSQRISF-SACSITLDGKLLN 63

Query: 68  DSTTISSSLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS 127
            ST I  S +P +S L L  R+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS
Sbjct: 64  GSTRIQVSKLPSVSMLTLFPRLRGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS 123

Query: 128 KWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKINLSMI 187
           KWLNCALSNEPL EPCVID LGN+FNKE LVHALL K+LPK F +IKGLKDM+ I L+ +
Sbjct: 124 KWLNCALSNEPLAEPCVIDLLGNLFNKEVLVHALLSKRLPKQFSYIKGLKDMVNIKLTPV 183

Query: 188 PGTESRGNAISEPRFQCPITGLEFNGKYKFFALRSCGHVLSAKALKEVKSSSCLVCHAEF 247
            G++      +  +FQCP++GLEFNGKYKFFALR CGHV+SAKALKEVKSSSCLVCHA+ 
Sbjct: 184 AGSDGSSQDTTSAQFQCPVSGLEFNGKYKFFALRGCGHVMSAKALKEVKSSSCLVCHADV 243

Query: 248 TVRDKFVINGSEEEVAEMRERMEEEKSKSKEKK--TKKVRNGEVGMNGDVNVDLASSRLS 307
              DK VING+EEEV  +RERMEEEK+K +EKK  +KK +NG                  
Sbjct: 244 KDSDKIVINGTEEEVDLLRERMEEEKAKLREKKGVSKKSKNG------------------ 303

Query: 308 GKKHGIEVKALEKVSAKPEKHERPDSGVQAKLAASNG-AVKRFKAADMAPANATKEVYAS 367
                     +    AK  K +  D  V       NG  VK+FKAAD  P NATKEVYAS
Sbjct: 304 -------AAVVADTGAKVAKRQIEDGNVN-----GNGITVKKFKAADKVPVNATKEVYAS 354

Query: 368 IFTSS-KKSDFKETFTCRSLPLGRN 387
           +FTSS KKSDF+ET++CRSLPLGRN
Sbjct: 364 LFTSSKKKSDFRETYSCRSLPLGRN 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3T1J81.1e-3135.79Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1[more]
Q99K953.5e-3036.03Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1[more]
Q5R9P97.9e-3034.74Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1[more]
Q9BY421.3e-2934.39Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3[more]
Q4R5941.7e-2934.91Replication termination factor 2 OS=Macaca fascicularis OX=9541 GN=RTF2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1F0679.8e-19390.41Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 P... [more]
A0A6J1KUM34.1e-19189.90Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=... [more]
A0A5A7SWS41.7e-18988.92Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B9F31.7e-18988.92Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV... [more]
A0A6J1C1362.1e-18787.56Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 ... [more]
Match NameE-valueIdentityDescription
XP_038879920.14.0e-19690.72replication termination factor 2 [Benincasa hispida][more]
XP_022933589.12.0e-19290.41protein RTF2 homolog [Cucurbita moschata][more]
KAG6587836.13.8e-19190.16Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7035670.15.0e-19189.90Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023531451.16.5e-19190.16protein RTF2 homolog [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G58020.14.8e-10758.44unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..284
NoneNo IPR availablePFAMPF04641Rtf2coord: 90..370
e-value: 2.8E-74
score: 249.8
NoneNo IPR availablePANTHERPTHR12775:SF1BNACNNG39770D PROTEINcoord: 7..386
IPR006735Replication termination factor 2PANTHERPTHR12775PROTEIN C20ORF43 HOMOLOGcoord: 7..386
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 9..90
score: 10.026781
IPR027799Replication termination factor 2, RING-fingerCDDcd16653RING-like_Rtf2coord: 200..245
e-value: 1.74189E-26
score: 98.0819

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g019280.1Lcy03g019280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902979 mitotic DNA replication termination
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding