Homology
BLAST of Lcy03g016430 vs. ExPASy Swiss-Prot
Match:
P41111 (Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus OX=9986 GN=EIF2B4 PE=2 SV=2)
HSP 1 Score: 335.1 bits (858), Expect = 1.8e-90
Identity = 203/448 (45.31%), Postives = 278/448 (62.05%), Query Frame = 0
Query: 205 KAERRALQEAQRAAKISAKGEANKSDTASGATAPGKAGKQSSTVKGGPATASVASADNRG 264
+AERRA QEA+RA K + KGE G P + P+TA A A G
Sbjct: 100 RAERRAKQEAERALKQARKGE-------QGGPPP----------QASPSTAGEAPA---G 159
Query: 265 GDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLP 324
G R+ + + D P + + V +++++ V ++ + ++V LF HLPQY + L
Sbjct: 160 GKRLTEHTQADD---PTLL---RRLVRKSERQQVPTRKDYGSKVSLFSHLPQYSRQNSLT 219
Query: 325 DLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRD 384
+S IHPA+ ++GLQY G ISG NARCIA+L A QQ I+DY+TPP + L RD
Sbjct: 220 QYMS--IPSSVIHPAMVRLGLQYSQGLISGSNARCIALLRALQQVIQDYTTPPNEELSRD 279
Query: 385 LTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEK 444
L K+ Y+ F +CRPLS SM NAI+FL I + + E EAKA L R++ EK
Sbjct: 280 LVNKLKPYICFLTQCRPLSASMYNAIKFLNKEITGVSSTKREEEAKAELQAAADRYVQEK 339
Query: 445 IMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQA 504
I+LA + I+R A+ KI +GDV+L YG S +V +L A G++FRVVVVDSRP+LEG+
Sbjct: 340 IVLAAQAILRFASKKISNGDVILVYGCSSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRH 399
Query: 505 LLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFH 564
+LR L+ G+ +Y I A SYV+ EV++V LGA A+LANG+V SRVGTA +A+VA A +
Sbjct: 400 MLRFLVRAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHN 459
Query: 565 VPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMY 624
VPVL CCE YKF ERVQ D+ NEL DPD + R D L + S L+LLNL+Y
Sbjct: 460 VPVLVCCETYKFCERVQTDAFVSNELDDPDDLQ--CERGDHVALANWQSHPSLRLLNLVY 517
Query: 625 DATPSDYISMIVTDYGMIPPTSVPVIVR 653
D TP + + +++T+ GMIP +SVPV++R
Sbjct: 520 DVTPPELVDLVITELGMIPCSSVPVVLR 517
BLAST of Lcy03g016430 vs. ExPASy Swiss-Prot
Match:
Q61749 (Translation initiation factor eIF-2B subunit delta OS=Mus musculus OX=10090 GN=Eif2b4 PE=1 SV=2)
HSP 1 Score: 329.3 bits (843), Expect = 9.8e-89
Identity = 208/477 (43.61%), Postives = 285/477 (59.75%), Query Frame = 0
Query: 176 GATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGA 235
G ++GG E LP+ KA+ +AERRA QEA+RA K + KGE
Sbjct: 78 GPGSQLGGTAGE---KLPAGRS--KAE-LRAERRAKQEAERALKQARKGE---QGGVPPQ 137
Query: 236 TAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKK 295
P AG+ +S VK P A P + + + + +
Sbjct: 138 ACPSTAGETTSGVKRVP--------------------EHTPADDPTLL---RRLLRKPDR 197
Query: 296 RAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGG 355
+ V ++ + ++V LF HLPQY + + L +S IHPA+ ++GLQY G ISG
Sbjct: 198 QQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPAMVRLGLQYSQGLISGS 257
Query: 356 NARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKN 415
NARCIA+LHA QQ I+DY+TPP + L RDL K+ Y+SF +CRP+S SM NAI+FL
Sbjct: 258 NARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTK 317
Query: 416 RIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVV 475
+ + S E EAK+ L + R++ EKI+LA + I R A+TKI DGDV+L YG S +V
Sbjct: 318 EVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLV 377
Query: 476 EMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVF 535
+L A G++FRVVVVDSRP+LEG+ +L L+ G+ +Y I A SYV+ EV++V
Sbjct: 378 SRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVL 437
Query: 536 LGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDA 595
LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+ NEL DPD
Sbjct: 438 LGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDD 497
Query: 596 ISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVR 653
+ R D L + S L+LLNL+YD TP + + +++T+ GMIP +SVPV++R
Sbjct: 498 LQ--CKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLR 518
BLAST of Lcy03g016430 vs. ExPASy Swiss-Prot
Match:
Q63186 (Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus OX=10116 GN=Eif2b4 PE=2 SV=1)
HSP 1 Score: 328.6 bits (841), Expect = 1.7e-88
Identity = 221/563 (39.25%), Postives = 300/563 (53.29%), Query Frame = 0
Query: 94 SHNPSETLLGSSPMASPSSRIAGEDLEFSEDSSASVLLRRSGSGKLASSCPGVGFHMAAK 153
S + +T L P A+ E L+ ++ R+ G V
Sbjct: 12 SRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVSAAQRQD 71
Query: 154 PAENLQTMPANSVRTLGTNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQE 213
P LQ + T G PAG ++ AE+ +AERRA QE
Sbjct: 72 PVRELQGTGSQLGGTTGEKLPAGRSK------AEL----------------RAERRAKQE 131
Query: 214 AQRAAKISAKGEANKSDTASGATAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKER 273
A+RA K + KGE S P AG+ +S VK
Sbjct: 132 AERALKQARKGE---QGGPSPQACPSTAGEATSGVK------------------------ 191
Query: 274 KKDAPPPRMQFDDKHRVERAKKRAVFSQTEAR----NRVELFRHLPQYEQGTQLPDLVSK 333
P Q DD + R ++ Q R ++V LF HLPQY + + L +S
Sbjct: 192 ---RVPEHTQADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS- 251
Query: 334 FFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKI 393
IHPA+ ++GLQY G +SG NARCIA+LHA QQ I+DY+TPP + L RDL K+
Sbjct: 252 -IPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKL 311
Query: 394 GVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLAD 453
Y+SF +CRP+S SM NAI+F + + S E EAK+ L I R++ EKI+LA
Sbjct: 312 KPYISFLTQCRPMSASMCNAIKFFNKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLAS 371
Query: 454 EVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRL 513
+ I R A+ KI DGDV+L YG S +V +L A G++FRVVVVDSRP+LEG+ +L L
Sbjct: 372 QAISRFASKKISDGDVILVYGCSSLVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCL 431
Query: 514 IAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLF 573
+ G+ +Y I A SYV+ EV++V LGA A+LANG+V SRVGTA +A+VA A +VPVL
Sbjct: 432 VRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLV 491
Query: 574 CCEAYKFHERVQLDSICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPS 633
CCE YKF ERVQ D+ NEL DPD + R D L + + L+LLNL+YD TP
Sbjct: 492 CCETYKFCERVQTDAFVSNELDDPDDLQ--CKRGDQVTLANWQNNSSLRLLNLVYDVTPP 518
Query: 634 DYISMIVTDYGMIPPTSVPVIVR 653
+ + +++T+ GMIP +SVPV++R
Sbjct: 552 ELVDLVITELGMIPCSSVPVVLR 518
BLAST of Lcy03g016430 vs. ExPASy Swiss-Prot
Match:
Q3T058 (Translation initiation factor eIF-2B subunit delta OS=Bos taurus OX=9913 GN=EIF2B4 PE=2 SV=1)
HSP 1 Score: 323.9 bits (829), Expect = 4.1e-87
Identity = 209/485 (43.09%), Postives = 285/485 (58.76%), Query Frame = 0
Query: 175 AGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASG 234
AG ++G A K+ +P+ KA+ +AERRA QEA+RA K + KG+
Sbjct: 78 AGPDSQLGTA----KEKVPAGRS--KAE-LRAERRAKQEAERAMKQARKGD--------- 137
Query: 235 ATAPGKAGKQSSTVKGGP---ATASVASADNRGGDRIVDKERKKDAPPPRMQFDD----K 294
+GGP A S A G R+ P Q DD +
Sbjct: 138 --------------QGGPPPQACPSTAGETPSGVKRL----------PEHSQVDDPTLLR 197
Query: 295 HRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQY 354
V++ +++ V ++ + ++V LF HLPQY + L +S IHPA+ ++GLQY
Sbjct: 198 RLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQY 257
Query: 355 MAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMG 414
G +SG NARCIA+L A QQ I+DY+TPP + L RDL K+ Y SF +CRPLS SM
Sbjct: 258 SQGLVSGSNARCIALLRALQQVIQDYTTPPSEELSRDLVNKLKPYFSFLTQCRPLSASMY 317
Query: 415 NAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLL 474
NAI+ L I + S E EAK+ L I R+I EKI+LA + I R A KI +GDV+L
Sbjct: 318 NAIKLLNKEITGVSGSKREEEAKSELRAAIDRYIKEKIVLAAQAISRFAYKKISNGDVIL 377
Query: 475 TYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYV 534
YG S +V +L A G+QFRVVVVDSRP+LE + LR L+ G+ +Y I A SYV
Sbjct: 378 VYGCSSLVSHILQEAWAKGRQFRVVVVDSRPRLEAKHTLRSLVRAGVPASYLLIPAASYV 437
Query: 535 MREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICF 594
+ EV++V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+
Sbjct: 438 LPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVS 497
Query: 595 NELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSV 653
NEL DPD + + R + L + + L+LLNL+YD TP + + +++T+ GMIP +SV
Sbjct: 498 NELDDPDDL--LCERGEHVALANWQNHSSLRLLNLVYDVTPPELVDLVITELGMIPCSSV 518
BLAST of Lcy03g016430 vs. ExPASy Swiss-Prot
Match:
Q9UI10 (Translation initiation factor eIF-2B subunit delta OS=Homo sapiens OX=9606 GN=EIF2B4 PE=1 SV=2)
HSP 1 Score: 322.4 bits (825), Expect = 1.2e-86
Identity = 223/539 (41.37%), Postives = 297/539 (55.10%), Query Frame = 0
Query: 134 SGSGKLASSCPG---VGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEKMGGAPAEVK- 193
SGSG A PG VG M + E LQ + + GA + G A + +
Sbjct: 12 SGSGMKAELPPGPGAVGREMTKE--EKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQC 71
Query: 194 -----DHLPSSSKPLKAKTTK-----------AERRALQEAQRAAKISAKGEANKSDTAS 253
LP S L K AERRA QEA+RA K + KGE +
Sbjct: 72 QVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKA 131
Query: 254 GATAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERA 313
+P AG+ S VK P V D+ R+V K ++ P
Sbjct: 132 ---SPSTAGETPSGVKRLPEYPQV---DDLLLRRLVKKPERQQVP--------------- 191
Query: 314 KKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDIS 373
++ + ++V LF HLPQY + L +S IHPA+ ++GLQY G +S
Sbjct: 192 ------TRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQYSQGLVS 251
Query: 374 GGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFL 433
G NARCIA+L A QQ I+DY+TPP + L RDL K+ Y+SF +CRPLS SM NAI+FL
Sbjct: 252 GSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL 311
Query: 434 KNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC 493
I + S E EAK+ L I R++ EKI+LA + I R A KI +GDV+L YG S
Sbjct: 312 NKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSS 371
Query: 494 VVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNR 553
+V +L A G++FRVVVVDSRP LEG+ LR L+ G+ +Y I A SYV+ EV++
Sbjct: 372 LVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSK 431
Query: 554 VFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDP 613
V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+ NEL DP
Sbjct: 432 VLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDP 491
Query: 614 DAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVR 653
D + R + L + + L+LLNL+YD TP + + +++T+ GMIP +SVPV++R
Sbjct: 492 DDLQ--CKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLR 517
BLAST of Lcy03g016430 vs. ExPASy TrEMBL
Match:
A0A6J1BZ97 (translation initiation factor eIF-2B subunit delta-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)
HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 618/661 (93.49%), Postives = 636/661 (96.22%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARR SRVVIDPKVRQVGFFTPDAP SPGRT APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1 MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61 PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGATEK
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
+GGA AEVKDH SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TAP K
Sbjct: 181 IGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTAPSK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRAVF+
Sbjct: 241 VGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEMLLS
Sbjct: 421 PPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLS 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS
Sbjct: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW 660
GRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW
Sbjct: 601 GRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW 660
Query: 661 V 662
V
Sbjct: 661 V 661
BLAST of Lcy03g016430 vs. ExPASy TrEMBL
Match:
A0A6J1BZT6 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)
HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 618/664 (93.07%), Postives = 636/664 (95.78%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARR SRVVIDPKVRQVGFFTPDAP SPGRT APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1 MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61 PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGAT-- 180
FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGAT
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATAL 180
Query: 181 -EKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATA 240
EK+GGA AEVKDH SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TA
Sbjct: 181 AEKIGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTA 240
Query: 241 PGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 300
P K GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRA
Sbjct: 241 PSKVGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRA 300
Query: 301 VFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360
VF+QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA
Sbjct: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360
Query: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420
RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI
Sbjct: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420
Query: 421 AKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEM 480
AKLPP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEM
Sbjct: 421 AKLPPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480
Query: 481 LLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLG 540
LLSYAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLG
Sbjct: 481 LLSYAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLG 540
Query: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600
AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS
Sbjct: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600
Query: 601 KVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 660
KVSGRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE
Sbjct: 601 KVSGRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 660
Query: 661 HLWV 662
HLWV
Sbjct: 661 HLWV 664
BLAST of Lcy03g016430 vs. ExPASy TrEMBL
Match:
A0A6J1F4E1 (translation initiation factor eIF-2B subunit delta-like OS=Cucurbita moschata OX=3662 GN=LOC111440216 PE=3 SV=1)
HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 612/662 (92.45%), Postives = 625/662 (94.41%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARRASR+VIDPKVRQVGFFTPDAPHSPG LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1 MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNL+SR+ V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61 PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
GGA E KDH PSSSKPLK KTTKAERRALQEAQR AK+SAKGEANKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATA-GK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ STVK GPAT SVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIF 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH-L 660
GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH L
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 660
Query: 661 WV 662
WV
Sbjct: 661 WV 660
BLAST of Lcy03g016430 vs. ExPASy TrEMBL
Match:
A0A6J1KJK1 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495813 PE=3 SV=1)
HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 606/662 (91.54%), Postives = 620/662 (93.66%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARRASRVVIDPKVRQVGFFTPDAPHSPG LP PPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPTPPDCTTSLLPDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNL+SR+A N RHPEDLV VGSHNPSE LL SSPMASPS+RIAGE+LE
Sbjct: 61 PRHPSDNLSSRSA-------GNWRHPEDLVRVGSHNPSEMLLRSSPMASPSTRIAGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
SE SSASVLLRRSGSGKLASSCPGVGF+MAAK +NL+TMPANSVRTLGTNKPA ATEK
Sbjct: 121 SSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAEATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
MGGA E KDHLPSSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS +ASGATA GK
Sbjct: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGSASGATA-GK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDK+RVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKNRVERAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRV LFRHLPQYEQGTQLPDLVSKFFQLDT HPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVALFRHLPQYEQGTQLPDLVSKFFQLDTFHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH-L 660
GR DI+YLDSLTSKEH QLLNLMYDATPSDYISMIVTDYGMIP TSVPVIVREYRREH L
Sbjct: 601 GRADISYLDSLTSKEHFQLLNLMYDATPSDYISMIVTDYGMIPATSVPVIVREYRREHLL 653
Query: 661 WV 662
WV
Sbjct: 661 WV 653
BLAST of Lcy03g016430 vs. ExPASy TrEMBL
Match:
A0A6J1BZS4 (translation initiation factor eIF-2B subunit delta-like OS=Momordica charantia OX=3673 GN=LOC111007076 PE=3 SV=1)
HSP 1 Score: 1021.9 bits (2641), Expect = 1.2e-294
Identity = 540/662 (81.57%), Postives = 582/662 (87.92%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARR SR+V +PKVRQVGFFT DAP+ P R+L P DCTTS S SP +NSLSPVMIPP
Sbjct: 1 MDARRGSRIV-NPKVRQVGFFTIDAPNPPSRSLSVPADCTTSPQSHSPLTNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRH SDNLASR+ V+PVPA AN R+ D +PVGS+NPSETLLG+SP+ASPSSRI E+L+
Sbjct: 61 PRHLSDNLASRSTVLPVPAPANWRNSADQIPVGSYNPSETLLGTSPVASPSSRIEDEELD 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
SEDS+ SV RRSGSGK+ASS PGVG H+AAK +EN Q +P + RT +KP G EK
Sbjct: 121 LSEDSATSVWYRRSGSGKIASSFPGVGIHVAAKSSENSQAVPLANARTSEPDKPTGVAEK 180
Query: 181 MGGAPAEV-KDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPG 240
GGA EV LP SSKPLKAKTTKAERRALQEAQRAAK+SAKGEANKS +TAPG
Sbjct: 181 NGGASTEVPNQQLPPSSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKS-----STAPG 240
Query: 241 KAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF 300
KA KQ S +K PA SVAS D RGGDR +DKERKKDAP PRMQFDDK+RVE+AKKRAVF
Sbjct: 241 KAVKQPSAIKDSPAAPSVASTDKRGGDRTLDKERKKDAPHPRMQFDDKNRVEKAKKRAVF 300
Query: 301 SQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARC 360
+QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDI GGNARC
Sbjct: 301 NQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDICGGNARC 360
Query: 361 IAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAK 420
IAMLHAFQQAIKDYSTPPQKSL RDLTAKIG YVSFFNECRPLSVSMGNAIRFLKNRIAK
Sbjct: 361 IAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAK 420
Query: 421 LPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLL 480
LP +L ESE KATLC DI+RFINEKI+LADEVIVRHAATKIRDGDVLLTYGSS VEMLL
Sbjct: 421 LPLNLPESEVKATLCSDINRFINEKIILADEVIVRHAATKIRDGDVLLTYGSSSAVEMLL 480
Query: 481 SYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAA 540
YAH+LGKQFRVVVVDSRPK+EGQALLRRLI +GLSCTYTHINAISYVM EV RVFLGAA
Sbjct: 481 LYAHELGKQFRVVVVDSRPKMEGQALLRRLITRGLSCTYTHINAISYVMHEVTRVFLGAA 540
Query: 541 AVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKV 600
AVL+NGTVYSRVGTAG+AMVA+AFHVPVL CCEAYKFHERVQLDSICFNELGDPDAISKV
Sbjct: 541 AVLSNGTVYSRVGTAGVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKV 600
Query: 601 SGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHL 660
GR +INYLD+LT+KE LQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH+
Sbjct: 601 PGREEINYLDNLTAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHV 656
Query: 661 WV 662
W+
Sbjct: 661 WI 656
BLAST of Lcy03g016430 vs. NCBI nr
Match:
XP_022134966.1 (translation initiation factor eIF-2B subunit delta-like isoform X2 [Momordica charantia])
HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 618/661 (93.49%), Postives = 636/661 (96.22%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARR SRVVIDPKVRQVGFFTPDAP SPGRT APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1 MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61 PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGATEK
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
+GGA AEVKDH SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TAP K
Sbjct: 181 IGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTAPSK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRAVF+
Sbjct: 241 VGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEMLLS
Sbjct: 421 PPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLS 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS
Sbjct: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW 660
GRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW
Sbjct: 601 GRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLW 660
Query: 661 V 662
V
Sbjct: 661 V 661
BLAST of Lcy03g016430 vs. NCBI nr
Match:
XP_022134965.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Momordica charantia])
HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 618/664 (93.07%), Postives = 636/664 (95.78%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARR SRVVIDPKVRQVGFFTPDAP SPGRT APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1 MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61 PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGAT-- 180
FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGAT
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATAL 180
Query: 181 -EKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATA 240
EK+GGA AEVKDH SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TA
Sbjct: 181 AEKIGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTA 240
Query: 241 PGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 300
P K GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRA
Sbjct: 241 PSKVGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRA 300
Query: 301 VFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360
VF+QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA
Sbjct: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360
Query: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420
RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI
Sbjct: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420
Query: 421 AKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEM 480
AKLPP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEM
Sbjct: 421 AKLPPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480
Query: 481 LLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLG 540
LLSYAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLG
Sbjct: 481 LLSYAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLG 540
Query: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600
AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS
Sbjct: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600
Query: 601 KVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 660
KVSGRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE
Sbjct: 601 KVSGRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 660
Query: 661 HLWV 662
HLWV
Sbjct: 661 HLWV 664
BLAST of Lcy03g016430 vs. NCBI nr
Match:
XP_023531306.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 616/662 (93.05%), Postives = 629/662 (95.02%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARRASRVVIDPKVRQVGFFTPDAPHSPG TLPAPPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNL+SR+AV+ N RHPEDLV VGSHNPSE LL SSPMAS S+RIAGE+LE
Sbjct: 61 PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
MGGA E KDHLPSSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASGATA GK
Sbjct: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATA-GK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV+RVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH-L 660
GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH L
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 655
Query: 661 WV 662
WV
Sbjct: 661 WV 655
BLAST of Lcy03g016430 vs. NCBI nr
Match:
XP_022933250.1 (translation initiation factor eIF-2B subunit delta-like [Cucurbita moschata])
HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 612/662 (92.45%), Postives = 625/662 (94.41%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARRASR+VIDPKVRQVGFFTPDAPHSPG LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1 MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNL+SR+ V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61 PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
GGA E KDH PSSSKPLK KTTKAERRALQEAQR AK+SAKGEANKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATA-GK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ STVK GPAT SVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIF 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH-L 660
GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH L
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 660
Query: 661 WV 662
WV
Sbjct: 661 WV 660
BLAST of Lcy03g016430 vs. NCBI nr
Match:
KAG6587629.1 (Translation initiation factor eIF-2B subunit delta, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 613/662 (92.60%), Postives = 625/662 (94.41%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 60
MDARRASRVVIDPKVRQVGFFTPDAPHSPG LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60
Query: 61 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 120
PRHPSDNL+SR V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61 PRHPSDNLSSRLVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120
Query: 121 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 180
FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK +NL+TMPANSVRTLGTNKPAG TEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGTTEK 180
Query: 181 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 240
GGA E KDH PSSSKPLK KTTKAERRALQEAQRAAK+S KGEANKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRAAKVSGKGEANKSGTASGATA-GK 240
Query: 241 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 300
GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
Query: 421 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 480
PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540
YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540
Query: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600
VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600
Query: 601 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH-L 660
GR DI+YLDSLTSKE+LQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH L
Sbjct: 601 GRADISYLDSLTSKENLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 660
Query: 661 WV 662
WV
Sbjct: 661 WV 660
BLAST of Lcy03g016430 vs. TAIR 10
Match:
AT5G38640.1 (NagB/RpiA/CoA transferase-like superfamily protein )
HSP 1 Score: 707.6 bits (1825), Expect = 9.4e-204
Identity = 407/677 (60.12%), Postives = 485/677 (71.64%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSD----SPTSNSLSPV 60
M++RR R V+DPKVRQVGFFT P S + P TS S SP+ NSLSPV
Sbjct: 1 MESRRNPRAVVDPKVRQVGFFTSSQPDSSTQQQQPDPIAATSASSSSSTISPSGNSLSPV 60
Query: 61 MIPPPR-HPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIA 120
MIPPPR + SD R AV ASA SPSS
Sbjct: 61 MIPPPRLNSSDTFPLRVAVAGAAASA--------------------------VSPSSFSY 120
Query: 121 GEDLEFSEDSSASVLLRRS------GSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTL 180
+F + S+ + R S S +ASS PG+G M + A ++PA+ + T+
Sbjct: 121 SSRRDFPDGSTTASPGRHSRGSFVKSSVPVASSLPGIG--MDSMAAAKSSSVPASGLTTV 180
Query: 181 G-TNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANK 240
P G +EK G + KPLK KTTKAERRA+QEAQRAAK +AK + K
Sbjct: 181 SVVTMPPGLSEKASGMEVQ-----SDQKKPLKEKTTKAERRAIQEAQRAAKAAAKADGGK 240
Query: 241 ---SDTASGATAPGKAGKQS-STVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQF 300
+AS A+ KA K + +T + + +A+ +GG +K+RKKDAP PRMQ+
Sbjct: 241 VAPVSSASVASTSVKAAKPAKATSQKNDVAVATGAAEKKGGPS--EKDRKKDAPHPRMQY 300
Query: 301 DDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVG 360
DD+ RV +AK+RAV QTEA+NRVELFRHLPQYE GTQLPDL +KFFQLD +HPA+YKVG
Sbjct: 301 DDESRVVKAKRRAVVKQTEAKNRVELFRHLPQYEHGTQLPDLETKFFQLDPMHPAVYKVG 360
Query: 361 LQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSV 420
LQY++GDISGGNARCIAML AFQ+ +KDYSTPP+KSL RD+TAKI YVSF ECRPLS+
Sbjct: 361 LQYLSGDISGGNARCIAMLQAFQEVVKDYSTPPEKSLNRDMTAKISSYVSFLIECRPLSI 420
Query: 421 SMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGD 480
SMGNAIRF+KNRIAKLP +L ESEAKATL DI RFINEKI++AD VIV+HA TKIRDGD
Sbjct: 421 SMGNAIRFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHAVTKIRDGD 480
Query: 481 VLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAI 540
VLLTYGS VEM++ +AH+L K+FRV+VVDSRPKLEGQ LLRRLI +G++CTYTHINAI
Sbjct: 481 VLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHINAI 540
Query: 541 SYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDS 600
SY+M EV +VFLGA++VL+NGTVYSRVGTA +AMVA+AF VPVL CCEAYKFHERVQLDS
Sbjct: 541 SYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHERVQLDS 600
Query: 601 ICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPP 660
IC NELGDP+AIS+V GR DINYLD LT+ +LQ LNLMYDATPSDYISMI+TDYGM+PP
Sbjct: 601 ICSNELGDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMIITDYGMVPP 642
Query: 661 TSVPVIVREYRREHLWV 662
TSVPVIVREY++EHL V
Sbjct: 661 TSVPVIVREYQKEHLLV 642
BLAST of Lcy03g016430 vs. TAIR 10
Match:
AT1G48970.1 (NagB/RpiA/CoA transferase-like superfamily protein )
HSP 1 Score: 681.8 bits (1758), Expect = 5.5e-196
Identity = 396/680 (58.24%), Postives = 491/680 (72.21%), Query Frame = 0
Query: 1 MDARRASRVVIDPKVRQVGFFTPDAP------HSPGRTLPAPPDCTTSQ--LSDSPTSNS 60
MD RR + V PKVR+VGFFT P P R+ P D TTS LSDSP+ N
Sbjct: 1 MDTRRGALAV--PKVRRVGFFTSIEPPPESSLQRPNRSQSGPVDATTSSSPLSDSPSGNL 60
Query: 61 LSPVMIPPPRHPSDNLASRTAV-----MPVPA-SANRRH--PE-DLVPVGSHNPSETLLG 120
+SPV+IPP RH SDNL SR A +PVP +A RR+ PE L+ VGS+NP ++LLG
Sbjct: 61 ISPVLIPPSRHHSDNLTSRVAAAAAAPVPVPGPAAFRRYLTPERTLLHVGSYNPPDSLLG 120
Query: 121 SSPMASPSSRIAGEDLEFSEDSSASVLLRRSGSGKLASSCPGVGFH--MAAKPAENLQTM 180
+S SPSS SEDS++ +RS S KL++S P GF +A + + +T
Sbjct: 121 TS---SPSSNGG-----LSEDSASLFGFQRSDSTKLSASLPNGGFDLTLAVRAPQESETK 180
Query: 181 PANSVRTLGTNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKIS 240
A + + G K A + K A K K LK KTTKAERRA+QEAQRAAK +
Sbjct: 181 IATTSASQGKKKIAEVSGK--SESATTKPQKEKEPKALKDKTTKAERRAIQEAQRAAKAA 240
Query: 241 AKGEANKSDTASGATAPGKAG-KQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPP 300
AKGE ++ SG PGKA K + P T+SV+ V+KE++ D P
Sbjct: 241 AKGEGSRRADESGRANPGKAAIKPQPKKERLPVTSSVSEKTAVA----VEKEKRMDVPQT 300
Query: 301 RMQFDDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAI 360
+MQ+DDK RV++AK+RAV QTE++N+VELF HLPQYE+ QLP+L S FF LD+IH A+
Sbjct: 301 QMQYDDKSRVDKAKRRAVVEQTESKNKVELFLHLPQYERSNQLPNLSSNFFTLDSIHHAV 360
Query: 361 YKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECR 420
YKVGLQ++AGDISG NARCIAML AFQ+AI+DYSTPP K L DLTAKI YVSF ECR
Sbjct: 361 YKVGLQHLAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECR 420
Query: 421 PLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKI 480
PLS+SMGNAIRFLKN+I KLP +L ESEAK++LC DI RFI+EK ++AD+ IV+HA TKI
Sbjct: 421 PLSMSMGNAIRFLKNQIRKLPVNLSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKI 480
Query: 481 RDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTH 540
RDG+VLLTYG SCVVEM+L YAH++GK+FRVV+VDSRP LEGQ LLRRL+ +GL CTYTH
Sbjct: 481 RDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTH 540
Query: 541 INAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERV 600
INAISY+MRE RVFLGA+++ +NGT+Y+RVGT+ IAMVA+AF VPV+ CCEAYKFHERV
Sbjct: 541 INAISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERV 600
Query: 601 QLDSICFNELGDPDAISKV-SGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDY 660
LDSIC NELGDPDA++ + S RT+ + ++ + ++LQ LNLMYD+TPS+YISMIV+DY
Sbjct: 601 LLDSICSNELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDY 660
BLAST of Lcy03g016430 vs. TAIR 10
Match:
AT2G44070.1 (NagB/RpiA/CoA transferase-like superfamily protein )
HSP 1 Score: 492.3 bits (1266), Expect = 6.2e-139
Identity = 242/325 (74.46%), Postives = 284/325 (87.38%), Query Frame = 0
Query: 336 IHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSF 395
+H A+YKVGL Y++ DISGGNARCIAML AFQ+ +KDYSTPP+ +L RD+TA+I YVSF
Sbjct: 1 MHSAVYKVGLHYLSWDISGGNARCIAMLQAFQEVVKDYSTPPENTLNRDMTAQISSYVSF 60
Query: 396 FNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRH 455
ECRPLS+SMGNAIRF+KNRIAKLP +L ESEAKA+L DI RFINEKI+ AD VIV H
Sbjct: 61 LIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIVEH 120
Query: 456 AATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLS 515
A TKIRDGDVLLTYGSS V+EM+L +AH+LGK+FRV VVDSRPKL+G+ LLRRLI +G++
Sbjct: 121 AVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKRGIN 180
Query: 516 CTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYK 575
CTYTHI AISY+M +V +VFLGA++V +NGTVYSRVGTA +AMVA+AF VPVL CCEAYK
Sbjct: 181 CTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 240
Query: 576 FHERVQLD-SICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISM 635
FHE+VQLD SIC NELGDP+AISKV GR DINYL+ LT+ L+ LNLMYDATPSDYISM
Sbjct: 241 FHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDYISM 300
Query: 636 IVTDYGMIPPTSVPVIVREYRREHL 660
I+TDYGM+PPTSVPVIVREY++EHL
Sbjct: 301 IITDYGMVPPTSVPVIVREYQKEHL 325
BLAST of Lcy03g016430 vs. TAIR 10
Match:
AT3G07300.1 (NagB/RpiA/CoA transferase-like superfamily protein )
HSP 1 Score: 89.7 bits (221), Expect = 9.3e-18
Identity = 68/233 (29.18%), Postives = 109/233 (46.78%), Query Frame = 0
Query: 423 SLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYA 482
S L + K + +++ I E I E I A I +V+LT GSS V L A
Sbjct: 170 SSLTRKLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 229
Query: 483 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVL 542
+ + FRV V + P+ +G L + L+A+GL T +A+ ++ VN V +GA AV+
Sbjct: 230 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 289
Query: 543 ANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKVSG 602
ANG V VG A+ A VP + ++K + + NEL P +
Sbjct: 290 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 349
Query: 603 RTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY 655
+D LD LQ++N +D P + +S+ +TD G P+ + ++ +Y
Sbjct: 350 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADY 399
BLAST of Lcy03g016430 vs. TAIR 10
Match:
AT3G07300.2 (NagB/RpiA/CoA transferase-like superfamily protein )
HSP 1 Score: 89.7 bits (221), Expect = 9.3e-18
Identity = 68/233 (29.18%), Postives = 109/233 (46.78%), Query Frame = 0
Query: 423 SLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYA 482
S L + K + +++ I E I E I A I +V+LT GSS V L A
Sbjct: 170 SSLTRKLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 229
Query: 483 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVL 542
+ + FRV V + P+ +G L + L+A+GL T +A+ ++ VN V +GA AV+
Sbjct: 230 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 289
Query: 543 ANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKVSG 602
ANG V VG A+ A VP + ++K + + NEL P +
Sbjct: 290 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 349
Query: 603 RTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY 655
+D LD LQ++N +D P + +S+ +TD G P+ + ++ +Y
Sbjct: 350 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADY 399
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P41111 | 1.8e-90 | 45.31 | Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus OX=9... | [more] |
Q61749 | 9.8e-89 | 43.61 | Translation initiation factor eIF-2B subunit delta OS=Mus musculus OX=10090 GN=E... | [more] |
Q63186 | 1.7e-88 | 39.25 | Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus OX=10116... | [more] |
Q3T058 | 4.1e-87 | 43.09 | Translation initiation factor eIF-2B subunit delta OS=Bos taurus OX=9913 GN=EIF2... | [more] |
Q9UI10 | 1.2e-86 | 41.37 | Translation initiation factor eIF-2B subunit delta OS=Homo sapiens OX=9606 GN=EI... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BZ97 | 0.0e+00 | 93.49 | translation initiation factor eIF-2B subunit delta-like isoform X2 OS=Momordica ... | [more] |
A0A6J1BZT6 | 0.0e+00 | 93.07 | translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Momordica ... | [more] |
A0A6J1F4E1 | 0.0e+00 | 92.45 | translation initiation factor eIF-2B subunit delta-like OS=Cucurbita moschata OX... | [more] |
A0A6J1KJK1 | 0.0e+00 | 91.54 | translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Cucurbita ... | [more] |
A0A6J1BZS4 | 1.2e-294 | 81.57 | translation initiation factor eIF-2B subunit delta-like OS=Momordica charantia O... | [more] |
Match Name | E-value | Identity | Description | |
XP_022134966.1 | 0.0e+00 | 93.49 | translation initiation factor eIF-2B subunit delta-like isoform X2 [Momordica ch... | [more] |
XP_022134965.1 | 0.0e+00 | 93.07 | translation initiation factor eIF-2B subunit delta-like isoform X1 [Momordica ch... | [more] |
XP_023531306.1 | 0.0e+00 | 93.05 | translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucurbita pe... | [more] |
XP_022933250.1 | 0.0e+00 | 92.45 | translation initiation factor eIF-2B subunit delta-like [Cucurbita moschata] | [more] |
KAG6587629.1 | 0.0e+00 | 92.60 | Translation initiation factor eIF-2B subunit delta, partial [Cucurbita argyrospe... | [more] |
Match Name | E-value | Identity | Description | |
AT5G38640.1 | 9.4e-204 | 60.12 | NagB/RpiA/CoA transferase-like superfamily protein | [more] |
AT1G48970.1 | 5.5e-196 | 58.24 | NagB/RpiA/CoA transferase-like superfamily protein | [more] |
AT2G44070.1 | 6.2e-139 | 74.46 | NagB/RpiA/CoA transferase-like superfamily protein | [more] |
AT3G07300.1 | 9.3e-18 | 29.18 | NagB/RpiA/CoA transferase-like superfamily protein | [more] |
AT3G07300.2 | 9.3e-18 | 29.18 | NagB/RpiA/CoA transferase-like superfamily protein | [more] |