Lcy03g015730 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g015730 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 6.9e-175
Identity = 305/495 (61.62%), Postives = 375/495 (75.76%), Query Frame = 0

Query: 28  RKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATMACEYGPLMYLRFGSVDVIVAASAA 87
           RK   L LPPGPKPWPI+GNL+ +G  P H + A MA  YGPLMYL+ G VDV+VAASA+
Sbjct: 26  RKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAAMAQTYGPLMYLKMGFVDVVVAASAS 85

Query: 88  VAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAPYGPRWRLLRKISTVHLFSSKALDD 147
           VA Q LK  D NF SRPPN+GA+++AYN QD+VFAPYGPRWR+LRKI +VHLFS+KALDD
Sbjct: 86  VAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDD 145

Query: 148 HRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVCLANAVGRAIIGQRVFGDG----DPKAD 207
            R V ++EV  L  A+  AG+ PV L +LL VC  NA+ R ++G+RVF DG    DP+A 
Sbjct: 146 FRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAA 205

Query: 208 RFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHKWFDDFLTKILDDNRNLLA 267
            F +MV ++MV++G FN GDF P L WLD+ GVA +MKK+H  FD FLT IL++++    
Sbjct: 206 EFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHARFDAFLTDILEEHK---- 265

Query: 268 DSGGSGGSQHQDLLSMLLSSK-DQAEDENEKLT--------------GTDTSASTVEWAL 327
              G    + +DLLS L+S K D A+++  KLT              GTDTS+STVEWA+
Sbjct: 266 ---GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNLFVAGTDTSSSTVEWAI 325

Query: 328 AELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPHMA 387
           AELIR PK+LAQAQQE+D VVGRD LV +LDL +L YL+A+VKETFRLHP TPLSLP +A
Sbjct: 326 AELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKETFRLHPSTPLSLPRIA 385

Query: 388 AESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 447
           +ESCEING+ IPKGSTLL++VWAI RDP  WADPLEFRP RFLPGGEK  VDV+GNDFEV
Sbjct: 386 SESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPGGEKPKVDVRGNDFEV 445

Query: 448 IPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGLSPDKLNMDEAFGLTLRRAQPL 504
           IPFGAGRRIC GM LG+RMVQ++ ATL+H+F+W L +G  P+ LNM+EA+GLTL+RA PL
Sbjct: 446 IPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLNMEEAYGLTLQRADPL 505

BLAST of Lcy03g015730 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.3e-170
Identity = 297/514 (57.78%), Postives = 379/514 (73.74%), Query Frame = 0

Query: 4   ISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATM 63
           +++++A  L L   +  HR      +    RLPPGP PWPI+GNL  +G  P H TL+ M
Sbjct: 6   LTILLATVLFLILRIFSHR----RNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAM 65

Query: 64  ACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAP 123
              YGP+++LR G VDV+VAAS +VAEQ LKI D NF SRPPN+GAK++AYN QD+VFAP
Sbjct: 66  VTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAP 125

Query: 124 YGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVCLAN 183
           YG RWRLLRKIS+VHLFS+KAL+D + V +EEV  L   + R G  PV+L +L+ +C+ N
Sbjct: 126 YGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVN 185

Query: 184 AVGRAIIGQRVFG-DGDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKK 243
           A+GR +IG+R+FG D D KAD F +MV ++M L+G FN GDF PSL+WLDL GVAG+MK+
Sbjct: 186 ALGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKR 245

Query: 244 VHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDE------------ 303
           +HK FD FL+ IL ++     D       +H D+LS L+S K    D             
Sbjct: 246 LHKRFDAFLSSILKEHEMNGQD------QKHTDMLSTLISLKGTDLDGDGGSLTDTEIKA 305

Query: 304 ---NEKLTGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQ 363
              N    GTDTSASTV+WA+AELIR P ++ +AQ+ELD VVGRD  V++ D+ +L YLQ
Sbjct: 306 LLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQ 365

Query: 364 AVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRP 423
           AV+KE FRLHPPTPLSLPH+A+ESCEING+HIPKGSTLL ++WAI RDP +W+DPL F+P
Sbjct: 366 AVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKP 425

Query: 424 NRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGL 483
            RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+ LGLR +Q LTATLV  FDW L  G+
Sbjct: 426 ERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGV 485

Query: 484 SPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVY 502
           +P+KLNM+E++GLTL+RA PLV+HP+PRL+P VY
Sbjct: 486 TPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of Lcy03g015730 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.4e-156
Identity = 283/509 (55.60%), Postives = 362/509 (71.12%), Query Frame = 0

Query: 16  SAVLLHRFLVLC----RKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATMACEYGPLM 75
           S +LL  F+V      R  +   LPPGP  WPI+GNL Q+G  P H TL  ++ +YGPL 
Sbjct: 10  STLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDALSKQYGPLF 69

Query: 76  YLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAPYGPRWRLL 135
            LR GSV+V+VA+S+AVA Q L+  D NF +RPPN+GA+++AYN QD+VFAPYGPRWR+L
Sbjct: 70  RLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRML 129

Query: 136 RKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAP--VDLWRLLTVCLANAVGRAI 195
           RK+ +VHLFS KALDD R V + EVA LV  + R  +    V+L + L VC  NA+ RA+
Sbjct: 130 RKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAM 189

Query: 196 IGQRVFGDGD---PKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHKW 255
           +G+RVF D D    +AD F  MV ++M L+G FN GDF P L WLDL GV G+MK++H+ 
Sbjct: 190 LGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRR 249

Query: 256 FDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL------------ 315
           +D FL +++++N+           ++  DLLS+L+  K+   +   KL            
Sbjct: 250 YDAFLDRVIEENQ---------ANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLLNL 309

Query: 316 --TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQAVVKE 375
              GTDTS+STVEW LAELIR P +L + Q ELDSV+GRD LV++ DLP L YLQAVVKE
Sbjct: 310 FTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKE 369

Query: 376 TFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRPNRFLP 435
           TFRLHP TPLSLP MA+E C +NG+ IPK +TLLV+VW+IGRD   W DPLEFRP+RFLP
Sbjct: 370 TFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLP 429

Query: 436 GGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGLSPDKL 495
           GGE+ HVDVKGNDFEVIPFGAGRRIC G+ LGLRMVQ +TAT+VH++DW+LP G    KL
Sbjct: 430 GGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKL 489

Query: 496 NMDEAFGLTLRRAQPLVLHPRPRLSPEVY 502
           +M+EA+GLTL+RA PL++ P PRLS + Y
Sbjct: 490 DMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of Lcy03g015730 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.2e-152
Identity = 268/485 (55.26%), Postives = 342/485 (70.52%), Query Frame = 0

Query: 35  LPPGPKPWPIVGNLLQIGGAPLHSTLATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLK 94
           LPPGP+ WP++GNL Q+G  P H T+  +A +YGPL  LRFG  +V+VAASA VA Q L+
Sbjct: 41  LPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVAAQFLR 100

Query: 95  IRDTNFLSRPPNAGAKYIAYNCQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEE 154
             D NF +RPPN+GA+++AYN QD+VFAPYG RWR LRK+  +HLFS+KALDD R V E 
Sbjct: 101 GHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREG 160

Query: 155 EVAVLVEAVGRAGEAPVDLWRLLTVCLANAVGRAIIGQRVFG-DGDPKADRFMAMVEQVM 214
           EVA++V  + R   A V L +   VC  N + RA IG RVF  DG   A  F  MV ++M
Sbjct: 161 EVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEGAREFKEMVVELM 220

Query: 215 VLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQH 274
            L+G FN GDF P+L WLD  GV  +MK++H+ +D+ +   +++ +      G + G   
Sbjct: 221 QLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPDGVAAGEHG 280

Query: 275 QDLLSMLLS---SKDQAEDENEKLT--------------GTDTSASTVEWALAELIRQPK 334
            DLLS+LL+    + + + + EK+T              GTDT++STVEWALAELIR P 
Sbjct: 281 NDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELIRHPD 340

Query: 335 MLAQAQQELDSVVGRDLLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPHMAAESCEING 394
           +L +AQ ELD+VVGR  LVS+ DLPRL YL AV+KETFRLHP TPLSLP  AAE CE++G
Sbjct: 341 VLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEECEVDG 400

Query: 395 FHIPKGSTLLVDVWAIGRDPKKWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRR 454
           + IPKG+TLLV+VWAI RDP +W DPL+++P+RFLPG   A VDVKG DF +IPFGAGRR
Sbjct: 401 YRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGLIPFGAGRR 460

Query: 455 ICVGMRLGLRMVQMLTATLVHSFDWALPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRL 502
           IC G+  GLRMV ++TATLVH FDW L NG +PDKLNM+EA+GLTL+RA PL++ P PRL
Sbjct: 461 ICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPLMVQPVPRL 520

BLAST of Lcy03g015730 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.3e-144
Identity = 258/530 (48.68%), Postives = 361/530 (68.11%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           M  + +I+  +  +  ++  +      ++   L+LPPGPK +P++GNL Q+G  P H  L
Sbjct: 3   MTSLDIILFISAIVFLSIYYYNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKP-HQAL 62

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
              +  YGPLM LR GSVD++VA+S +VA Q LK  D+NF +RPPN+GA+++A+N  D+V
Sbjct: 63  QAFSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDLV 122

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAV---GRAGEAPVDLWRLL 180
           FAPYGPRWRLLRK+S VHL   KALDD++ V EEE+AVL   +    R GE PV++ + +
Sbjct: 123 FAPYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGE-PVNVGKEM 182

Query: 181 TVCLANAVGRAIIGQRVF-----GDGD-------PKADRFMAMVEQVMVLSGKFNPGDFF 240
            VC  NA+ RA++G+RVF     G G         KA+ F  MV +VM L+G FN GDF 
Sbjct: 183 HVCSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFV 242

Query: 241 PSLEWLDLLGVAGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKD 300
           P L+  D+ GV  +MK+VH+ ++ FL K        +A+   S      DLLS+L+  + 
Sbjct: 243 PWLKPFDIQGVVRKMKRVHRRYNVFLDK-------FIAECRSSAKPGANDLLSVLIGQRG 302

Query: 301 QAEDENEKLT--------------GTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGR 360
           +++    ++T              GTDTS+ST+EWAL ELIR P +L +AQQE+DS VGR
Sbjct: 303 KSDGSGGEITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGR 362

Query: 361 DLLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWA 420
           D LV++ D+P+L YLQA+VKE FR+HP TPLSLP M+ E C+I G+HIPK STL V++WA
Sbjct: 363 DRLVTESDVPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWA 422

Query: 421 IGRDPKKWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQML 480
           +GRDP  W DP+EFRP+RFLPGG+  H++V+GN FE++PFGAGRRIC G  +G+R+V   
Sbjct: 423 MGRDPSIWPDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHST 482

Query: 481 TATLVHSFDWALPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVY 502
            ATL+H+FDW LP GL+ +K++M+EAFG++L++A PL+ HP PRL+P+ Y
Sbjct: 483 VATLIHAFDWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of Lcy03g015730 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.0e-234
Identity = 411/520 (79.04%), Postives = 450/520 (86.54%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLAS VLL+R L   R  R+L LPPGPKPWPIVGNL QIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGSVDV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRRV EEEV VLV+A+ R+GEAP  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPGDFF  LEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           AGEMKKVHKWFDD ++KIL+++RNLLA     GG +HQDLLS LLS++D+A+DENEKL  
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLAT---GGGGEHQDLLSTLLSTRDRAKDENEKLND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDSVVGRD +VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEI+G+HIPKGSTLLVDVWAI RDPK+W  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQMLTA+LVHSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of Lcy03g015730 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 7.0e-231
Identity = 407/520 (78.27%), Postives = 445/520 (85.58%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLA+ VLL+R L   R  R+L LPPGPKPWP VGNL QIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGS+DV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+D+R V EEEV VLV+A+ R GEAP  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPG+FFPSLEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           A EMKKVHKWFDD L+KIL+++R LLA      G +HQDLLS LLS++D AEDENEKL  
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRTLLA-----SGGEHQDLLSTLLSTRDHAEDENEKLND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDSVVGRD +VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEING+HIPKGSTLLVDVWAI RDPK+W  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQMLTA+LVHSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of Lcy03g015730 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 402/524 (76.72%), Postives = 449/524 (85.69%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           M+EI+LIVAA +TLASA LLHR   L R+F   RLPPGPKPWPIVGN+ QIGGAPLHS L
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRF---RLPPGPKPWPIVGNMPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A MA +YGPLMYLR GSVD++VAASAAVAEQ LKI DTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKALDDHRR+ ++E++VLV+A+ R+GEAP +L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGDG----DPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
           +AN++GR +IG+RVFGDG    D ++++F AMVEQVMVL+GKFNPGDFFP LE LDL+GV
Sbjct: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLA-DSGGSGGSQHQDLLSMLLSSKDQAEDENEKL- 300
             EMKKVHKWFDDFLTKI++++RNLLA   GGSGG  HQDLLS LLS KD+  DENEKL 
Sbjct: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300

Query: 301 -------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLP 360
                         GTDTSASTVEWAL ELIR P+M+AQAQQELDSVVGRD  VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360

Query: 361 RLGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWAD 420
           +L YLQAVVKETFRLHPPTPLSLP MA++SCE+NG+HIPKGSTLLVDVWAIGRDPK+W D
Sbjct: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420

Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDW 480
           PLEF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+ LGLRMVQMLTAT+VHSFDW
Sbjct: 421 PLEFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480

Query: 481 ALPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYRGLP 506
           ALPNGL+PDKLNMDE +GLTLRRAQPLV+HPRPRL   VY+ LP
Sbjct: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of Lcy03g015730 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 402/524 (76.72%), Postives = 449/524 (85.69%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           M+EI+LIVAA +TLASA LLHR   L R+F   RLPPGPKPWPIVGN+ QIGGAPLHS L
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRF---RLPPGPKPWPIVGNMPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A MA +YGPLMYLR GSVD++VAASAAVAEQ LKI DTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKALDDHRR+ ++E++VLV+A+ R+GEAP +L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGDG----DPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
           +AN++GR +IG+RVFGDG    D ++++F AMVEQVMVL+GKFNPGDFFP LE LDL+GV
Sbjct: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLA-DSGGSGGSQHQDLLSMLLSSKDQAEDENEKL- 300
             EMKKVHKWFDDFLTKI++++RNLLA   GGSGG  HQDLLS LLS KD+  DENEKL 
Sbjct: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300

Query: 301 -------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLP 360
                         GTDTSASTVEWAL ELIR P+M+AQAQQELDSVVGRD  VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360

Query: 361 RLGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWAD 420
           +L YLQAVVKETFRLHPPTPLSLP MA++SCE+NG+HIPKGSTLLVDVWAIGRDPK+W D
Sbjct: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420

Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDW 480
           PLEF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+ LGLRMVQMLTAT+VHSFDW
Sbjct: 421 PLEFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480

Query: 481 ALPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYRGLP 506
           ALPNGL+PDKLNMDE +GLTLRRAQPLV+HPRPRL   VY+ LP
Sbjct: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of Lcy03g015730 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.7e-208
Identity = 367/498 (73.69%), Postives = 416/498 (83.53%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           M+EI+ IVAA +TLASA LLHR   L R+   LRLPPGPKPWPIVGN+ QIG A LHS L
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRR---LRLPPGPKPWPIVGNMPQIGEAHLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           ATMA +YGPLMYLR GSVD++VAASAAVAEQ LKI DTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  ATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKISTVHLFSSKALDDHRR+ EEE++VLV+A+ R+GEAP +L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGDG----DPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
           +AN++GR +IG+RVFGDG    D ++++F  MVEQVMVL+GKFNPGDFFP LEWLDL+GV
Sbjct: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKLTG 300
             EMKKVHKWFDDFLTKI++++RNLLA   G G   HQDLLS LLS KD  EDENEKL  
Sbjct: 241 GREMKKVHKWFDDFLTKIVEEHRNLLARGVGGG---HQDLLSTLLSMKDDGEDENEKLND 300

Query: 301 TDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQAVVKETFRL 360
           T+  A  +   L++              L+  +GRD  VSD+DL +L YLQAVVKETFRL
Sbjct: 301 TEIKALLLVLFLSKFY------------LNCFIGRDRAVSDVDLHQLVYLQAVVKETFRL 360

Query: 361 HPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRPNRFLPGGEK 420
           HPPTPLSLP MA++SCE+NG+HIPKGSTLLVDVWAIGRDPK+W DPLEFRPNRFLP GEK
Sbjct: 361 HPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEK 420

Query: 421 AHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGLSPDKLNMDE 480
            HVDVKGNDFEVIPFGAGRRICVG+ LGLRMVQMLTAT+VHSFDW LPNGL+PDKLNMDE
Sbjct: 421 PHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDE 480

Query: 481 AFGLTLRRAQPLVLHPRP 495
            +GLTLRRAQPL++HPRP
Sbjct: 481 HYGLTLRRAQPLIMHPRP 480

BLAST of Lcy03g015730 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 829.7 bits (2142), Expect = 1.4e-236
Identity = 412/520 (79.23%), Postives = 452/520 (86.92%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLAS VLL+R L   R  R+L LPPGPKPWPIVGNL QIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGSVDV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRRV EEEV VLV+A+ R+GEAP  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPGDFFPSL+WLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           A EMKKVHKWFDD L+KIL+++RNLLA  GG GG +HQDLLS LLS++D+AEDENEKL  
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDS+VGRD +VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLPK 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEI+G+ IPKGSTLLVDVWAI RDPK+W  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQ+LTA+LVHSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of Lcy03g015730 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 820.5 bits (2118), Expect = 8.2e-234
Identity = 411/520 (79.04%), Postives = 450/520 (86.54%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLAS VLL+R L   R  R+L LPPGPKPWPIVGNL QIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGSVDV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRRV EEEV VLV+A+ R+GEAP  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPGDFF  LEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           AGEMKKVHKWFDD ++KIL+++RNLLA     GG +HQDLLS LLS++D+A+DENEKL  
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLAT---GGGGEHQDLLSTLLSTRDRAKDENEKLND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDSVVGRD +VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEI+G+HIPKGSTLLVDVWAI RDPK+W  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQMLTA+LVHSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of Lcy03g015730 vs. NCBI nr
Match: KAG6587576.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.1 bits (2112), Expect = 4.1e-233
Identity = 410/520 (78.85%), Postives = 448/520 (86.15%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLAS VLL+R L   R  R+L LPPGPKPWPIVGNL QIGGAPLHS L
Sbjct: 92  MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 151

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGSVDV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 152 ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 211

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRRV EEEV VLV+A+ R GEAP  L  LLTVC
Sbjct: 212 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARPGEAPATLGSLLTVC 271

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPGDFFP LEWLDLLGV
Sbjct: 272 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPWLEWLDLLGV 331

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           A EMKKVHKWFDD ++KIL+++RNLLA     GG +HQDLL+ LLS++D A+DENEKL  
Sbjct: 332 AREMKKVHKWFDDLMSKILEEHRNLLAT---GGGGEHQDLLTTLLSTRDHAKDENEKLND 391

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDSVVGRD +VSDLDLP+
Sbjct: 392 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 451

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEI+G+HIPKGSTLLVDVWAI RDPK+W  P
Sbjct: 452 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSP 511

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQMLTA+LVHSFDWA
Sbjct: 512 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 571

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 572 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 608

BLAST of Lcy03g015730 vs. NCBI nr
Match: XP_023007386.1 (flavonoid 3'-monooxygenase [Cucurbita maxima])

HSP 1 Score: 809.7 bits (2090), Expect = 1.4e-230
Identity = 407/520 (78.27%), Postives = 445/520 (85.58%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEISL  +A +TLA+ VLL+R L   R  R+L LPPGPKPWP VGNL QIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A M+ EYGPLMYLRFGS+DV+VAASAA+AEQ LKIRDTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+D+R V EEEV VLV+A+ R GEAP  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGD----GDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGD    GDP +D+F AMVEQVM+LSGKFNPG+FFPSLEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           A EMKKVHKWFDD L+KIL+++R LLA      G +HQDLLS LLS++D AEDENEKL  
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRTLLA-----SGGEHQDLLSTLLSTRDHAEDENEKLND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWALAELIR PKM+ QAQQELDSVVGRD +VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPK 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           LGYLQAVVKETFRLHPPTPLSLP MAAESCEING+HIPKGSTLLVDVWAI RDPK+W  P
Sbjct: 361 LGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGM +GLRMVQMLTA+LVHSFDWA
Sbjct: 421 LEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYR 503
           LP+GLSP +LNMDEAFGLTLRRAQPLV HPRPRLSP VY+
Sbjct: 481 LPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of Lcy03g015730 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 809.7 bits (2090), Expect = 1.4e-230
Identity = 410/523 (78.39%), Postives = 445/523 (85.09%), Query Frame = 0

Query: 1   MDEISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTL 60
           MDEI+LIVAA +TLAS +LLHR      +F   RLPPGPKPWPIVGN+ QIGGAPLHS L
Sbjct: 1   MDEIALIVAAVVTLASTILLHRTFFRRSRF---RLPPGPKPWPIVGNMPQIGGAPLHSLL 60

Query: 61  ATMACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMV 120
           A MA EYG LMYLR GSV V+VAASAAVAE+ LK+ DTNFLSRPPNAGAKYIAYN QDMV
Sbjct: 61  AAMAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVC 180
           FAPYGPRWRLLRKISTVHLFSSKALDDHRR+ EEE+AVLV+A+  +GE P  L  LLTVC
Sbjct: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVC 180

Query: 181 LANAVGRAIIGQRVFGDG----DPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGV 240
            ANA+GR +IG+RVFGDG    D +A +F AMVEQVM+L+GKFNPGDFFPSLEWLDLLGV
Sbjct: 181 TANALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGV 240

Query: 241 AGEMKKVHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 300
           A EMKKVHKWFDDFLTKI++++RNLLA   G G   HQDLLS LLSS   AEDENEK+  
Sbjct: 241 ASEMKKVHKWFDDFLTKIVEEHRNLLARGRGGG---HQDLLSTLLSS---AEDENEKIND 300

Query: 301 ------------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPR 360
                        GTDTSASTVEWAL ELIR PKM+AQAQQELDSVVGRD  VSDLDLP+
Sbjct: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQ 360

Query: 361 LGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADP 420
           L YLQAVVKETFRLHPPTPLSLP MAAESCEING+HIPKG+TLLVDVWAIGRDPK+WADP
Sbjct: 361 LVYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADP 420

Query: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWA 480
           LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGM +GLRMVQMLTATLVH FDWA
Sbjct: 421 LEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWA 480

Query: 481 LPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVYRGLP 506
           LPNGL PD+LNMDEA+GLTLRRAQPLV+HPRPRL P VY+  P
Sbjct: 481 LPNGLLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514

BLAST of Lcy03g015730 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 600.1 bits (1546), Expect = 1.6e-171
Identity = 297/514 (57.78%), Postives = 379/514 (73.74%), Query Frame = 0

Query: 4   ISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATM 63
           +++++A  L L   +  HR      +    RLPPGP PWPI+GNL  +G  P H TL+ M
Sbjct: 6   LTILLATVLFLILRIFSHR----RNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAM 65

Query: 64  ACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAP 123
              YGP+++LR G VDV+VAAS +VAEQ LKI D NF SRPPN+GAK++AYN QD+VFAP
Sbjct: 66  VTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAP 125

Query: 124 YGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVCLAN 183
           YG RWRLLRKIS+VHLFS+KAL+D + V +EEV  L   + R G  PV+L +L+ +C+ N
Sbjct: 126 YGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVN 185

Query: 184 AVGRAIIGQRVFG-DGDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKK 243
           A+GR +IG+R+FG D D KAD F +MV ++M L+G FN GDF PSL+WLDL GVAG+MK+
Sbjct: 186 ALGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKR 245

Query: 244 VHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDE------------ 303
           +HK FD FL+ IL ++     D       +H D+LS L+S K    D             
Sbjct: 246 LHKRFDAFLSSILKEHEMNGQD------QKHTDMLSTLISLKGTDLDGDGGSLTDTEIKA 305

Query: 304 ---NEKLTGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQ 363
              N    GTDTSASTV+WA+AELIR P ++ +AQ+ELD VVGRD  V++ D+ +L YLQ
Sbjct: 306 LLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQ 365

Query: 364 AVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRP 423
           AV+KE FRLHPPTPLSLPH+A+ESCEING+HIPKGSTLL ++WAI RDP +W+DPL F+P
Sbjct: 366 AVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKP 425

Query: 424 NRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGL 483
            RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+ LGLR +Q LTATLV  FDW L  G+
Sbjct: 426 ERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGV 485

Query: 484 SPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVY 502
           +P+KLNM+E++GLTL+RA PLV+HP+PRL+P VY
Sbjct: 486 TPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of Lcy03g015730 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 332.0 bits (850), Expect = 8.2e-91
Identity = 192/515 (37.28%), Postives = 286/515 (55.53%), Query Frame = 0

Query: 6   LIVAATL--TLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATM 65
           ++V A+L   L   VLL R+L      +  RLPPGP   PI+GNLLQ+G  P H  LA++
Sbjct: 2   ILVLASLFAVLILNVLLWRWL-KASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASL 61

Query: 66  ACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAP 125
             +YGPL+YLR G+VD I         + L  +D  F SRP    A ++AY C D+  AP
Sbjct: 62  CDKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAP 121

Query: 126 YGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEA--PVDLWRLLTVCL 185
            GP W+ +R+I   HL ++K L+       EE   L+  V +  E   P++L  +L    
Sbjct: 122 MGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFS 181

Query: 186 ANAVGRAIIGQRVFGDG---DPK-ADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVA 245
            N V R ++G++ FG G    PK A  F+ +  ++  L G    GD+ P   W+D  G  
Sbjct: 182 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 241

Query: 246 GEMKKVHKWFDDFLTKILDDNRNL-LADSGGSGGSQHQDLLSMLLSSKDQAEDENEKL-- 305
            EM+ V K  D+F TKI+D++R   L D   +G     D+L  L     +A  E+ ++  
Sbjct: 242 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKA 301

Query: 306 -------TGTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQ 365
                    TDTSA T EWA+AE I+QP+++ + Q+ELD+VVG + +V + DL  L YL+
Sbjct: 302 LIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLR 361

Query: 366 AVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRP 425
            VV+ETFR+HP  P  +PH +  +  ING++IP  + + ++   +GR+ K W D  +FRP
Sbjct: 362 CVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRP 421

Query: 426 NRFLPGGEKAHVDVK-GNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNG 485
            R  P      V++  G DF+++PF AG+R C G  LG+ MV M  A L H F+W+    
Sbjct: 422 ERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWS---- 481

Query: 486 LSPDKLNMDEAFGLTLRRAQPLVLHPRPRLSPEVY 502
            SP  ++  E +G+T+ +A+PL    +PRL+  +Y
Sbjct: 482 -SPGNIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 509

BLAST of Lcy03g015730 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 322.0 bits (824), Expect = 8.5e-88
Identity = 190/518 (36.68%), Postives = 274/518 (52.90%), Query Frame = 0

Query: 4   ISLIVAATLTLASAVLLHRFLVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATM 63
           +S +   T +L   V L  F+    + R    PPGP+ WPI+GN+L +     H  LA +
Sbjct: 9   LSKLSDPTTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQL-THRGLANL 68

Query: 64  ACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAP 123
           A +YG L +LR G + +   +S  VA Q L+++D+ F +RP      Y+ Y+  DM FA 
Sbjct: 69  AKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAH 128

Query: 124 YGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVCLAN 183
           YGP WR +RK+  + +FS K  +    V  +EV  +V +V      P+++   +     N
Sbjct: 129 YGPFWRQMRKVCVMKVFSRKRAESWASV-RDEVDKMVRSVSCNVGKPINVGEQIFALTRN 188

Query: 184 AVGRAIIGQRVFGDGDPKADRFMAMVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKV 243
              RA  G       +   D F+ ++++   L G FN  DF P   W+D  G+   + K 
Sbjct: 189 ITYRAAFGSAC----EKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKA 248

Query: 244 HKWFDDFLTKILD------DNRNLLADSGGSGGSQHQDLLSML-----LSSKDQAEDENE 303
               D F+  I+D      +N+N + D          DLL+       L S+      + 
Sbjct: 249 RNDLDGFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSI 308

Query: 304 KLT--------------GTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRDLLVSDL 363
           KLT              GT+T AS +EWAL EL+R P+ L + QQEL  VVG D  V + 
Sbjct: 309 KLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEES 368

Query: 364 DLPRLGYLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKK 423
           D+ +L YL+  +KET R+HPP PL L H  AE   I+GF IPK S ++++ +AIGRDP  
Sbjct: 369 DIEKLTYLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTS 428

Query: 424 WADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHS 483
           W DP  FRP+RFL  G     D KG++FE IPFG+GRR C GM+LGL  + +  A ++H 
Sbjct: 429 WTDPDTFRPSRFLEPGVP---DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHC 488

Query: 484 FDWALPNGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRL 497
           F W LP+G+ P +L+M++ FGLT  +A  L   P  RL
Sbjct: 489 FTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of Lcy03g015730 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 313.9 bits (803), Expect = 2.3e-85
Identity = 176/481 (36.59%), Postives = 270/481 (56.13%), Query Frame = 0

Query: 31  RTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATMACEYGPLMYLRFGSVDVIVAASAAVAE 90
           R   LPPGP   PI+GN+ Q+G  P HS+ A +A  YGP+M L+FG ++ +V  S   A 
Sbjct: 34  RAATLPPGPPRLPIIGNIHQVGKNP-HSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAR 93

Query: 91  QTLKIRDTNFLSRPPNAGAKYIAYNCQDMVF-APYGPRWRLLRKISTVHLFSSKALDDHR 150
           + L+  D     R  N   +   +    +++  P   RWR+LRK+S   +FS +  +  +
Sbjct: 94  EVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATK 153

Query: 151 RVHEEEVAVLVEAVGRAGE--APVDLWRLLTVCLANAVGRAIIGQRVFGDGDPKADRFMA 210
            +  ++V  LV  +  + E    VD+ R     + N +   +    +      K++ F  
Sbjct: 154 ALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQD 213

Query: 211 MVEQVMVLSGKFNPGDFFPSLEWLDLLG----VAGEMKKVHKWFDDFL-TKILDDNRNLL 270
            V   M  +GK +  ++FP + +LDL G    + G  +++ + F  F+  KI + +    
Sbjct: 214 TVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEKSL--- 273

Query: 271 ADSGGSGGSQHQDLLS--MLLSSKDQAEDENEKL---------TGTDTSASTVEWALAEL 330
                S    ++D L   ++L+  D+AE +N  +          GTDTS+ST+EWA+AEL
Sbjct: 274 --GNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAEL 333

Query: 331 IRQPKMLAQAQQELDSVVGRDLLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPHMAAES 390
           +R PK + +AQ E+D V+G++ +V + D+  L YLQAVVKETFRLHP  PL +P  A   
Sbjct: 334 LRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESD 393

Query: 391 CEINGFHIPKGSTLLVDVWAIGRDPKKWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPF 450
            E+ GF +PK + +LV+VWAIGRDP  W +P +F P RF+       +DVKG D+E+ PF
Sbjct: 394 VEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM----GKDIDVKGRDYELTPF 453

Query: 451 GAGRRICVGMRLGLRMVQMLTATLVHSFDWALPNGLSPDKLNMDEAFGLTLRRAQPLVLH 493
           G GRRIC G+ L ++ V ++ A+L++SFDW LPNG+  + L+MDE FG+TL R   L   
Sbjct: 454 GGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAI 504

BLAST of Lcy03g015730 vs. TAIR 10
Match: AT5G04330.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 2.3e-85
Identity = 181/512 (35.35%), Postives = 277/512 (54.10%), Query Frame = 0

Query: 6   LIVAATLTLASAVLLHRF--LVLCRKFRTLRLPPGPKPWPIVGNLLQIGGAPLHSTLATM 65
           ++   TL +   +LL  F  L+L R+      PPGPK  P++GN+L +     H  LA +
Sbjct: 1   MLTLMTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFN-HRGLAKL 60

Query: 66  ACEYGPLMYLRFGSVDVIVAASAAVAEQTLKIRDTNFLSRPPNAGAKYIAYNCQDMVFAP 125
           +  YG L++LR G   + V +S  +A Q L+++D  F +RP     +Y+ Y   D+ F  
Sbjct: 61  SRIYGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCN 120

Query: 126 YGPRWRLLRKISTVHLFSSKALDDHRRVHEEEVAVLVEAVGRAGEAPVDLWRLLTVCLAN 185
           YGP WR +RK+  + LFS K  +    V +EEV   V  V      P+++ +L      +
Sbjct: 121 YGPFWRRMRKLYVMMLFSRKRAESWVSV-DEEVHKSVRLVASNVGKPLNICKLAFSLSRD 180

Query: 186 AVGRAIIGQRVFGDGDPKADRFMAMVEQVMVLSGKFNPGDFFPS-LEWLDLLGVAGEMKK 245
              RA  G       + + D F+ ++++   L G+FN  D+ PS L W+D  G+ G ++K
Sbjct: 181 ITFRAAFGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEK 240

Query: 246 VHKWFDDFLTKILDDNRNLLADSGGSGGSQHQDLLSMLLSSKDQAEDENEKLT------- 305
             K  D F+  ++DD+ +           +  D++  LL+  ++    N  +T       
Sbjct: 241 ARKSLDGFIESVIDDHLH-KKKREHDNVDEETDMVDQLLAFYEEEVKVNNSVTKINLDNI 300

Query: 306 ----------GTDTSASTVEWALAELIRQPKMLAQAQQELDSVVGRD-LLVSDLDLPRLG 365
                     GT+T A  +EW L E++R P+ + + Q EL SVVG D   V D  L +L 
Sbjct: 301 KGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDRWRVEDTHLEKLT 360

Query: 366 YLQAVVKETFRLHPPTPLSLPHMAAESCEINGFHIPKGSTLLVDVWAIGRDPKKWADPLE 425
           +L+ ++KET RLHPP PL L H   +  EI+G+ IPKGS ++V+ +A+GRDP  W+DP  
Sbjct: 361 FLKCILKETLRLHPPFPLLL-HETVKDTEISGYFIPKGSRVMVNTYALGRDPNSWSDPES 420

Query: 426 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMRLGLRMVQMLTATLVHSFDWALP 485
           F P RFL        D+KGN+FE +PFG+GRR C GM+LGL   ++  A L+H F W+LP
Sbjct: 421 FNPGRFL---NPIAPDLKGNNFEFVPFGSGRRSCPGMQLGLYAFELAVAHLLHCFTWSLP 480

Query: 486 NGLSPDKLNMDEAFGLTLRRAQPLVLHPRPRL 497
           +G++P  ++  E  GLT+ +A PLV  P  RL
Sbjct: 481 DGMNPGDVDTVEGPGLTVPKAIPLVAVPTTRL 505