Lcy03g015720 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g015720 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 4.9e-192
Identity = 331/520 (63.65%), Postives = 405/520 (77.88%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           M+ +SLI+     +     +L  F    R    L LPPGPKPWPI+GNL  +G  P H  
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFF---RKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQS 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
            AAMA  YGPLMYL+ G VDVVVAASA+VA QFLK  D NF SRPPN+GA+++AYNYQD+
Sbjct: 61  TAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDL 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWR+LRKI +VHLFS+KALDD R VR++EV  L + LASAG+ P  LG LL +
Sbjct: 121 VFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CT NAL R M+G+RVF DGS   DP+  +FK++V ++MV++G FN GDF P L WLD+ G
Sbjct: 181 CTTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDT 300
           VA +MKK+H  FD FLT IL+EH+  + G    + +DLLS L+S K D A+++  KL+DT
Sbjct: 241 VAAKMKKLHARFDAFLTDILEEHKGKIFG----EMKDLLSTLISLKNDDADNDGGKLTDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRL 360
           EIKALLLN+F AGTDTS+STVEWA+AELIR+PK+LAQ QQE+D VVGRDRLV +LDL +L
Sbjct: 301 EIKALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQL 360

Query: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPL 420
            YL+A+VKETFRLHP TPLSLPR+A+ESCEING+ IPKGSTLL++VWAI RDP  WADPL
Sbjct: 361 TYLEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWAL 480
           EFRP RFLPGGEK  VDV+GNDFEVIPFGAGRRIC GM+LG+R VQ++ ATL+H+F+W L
Sbjct: 421 EFRPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDL 480

Query: 481 PIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQG 520
             G  P+ LNM+EA+GLTL+RA PL +HPRPRL  + Y G
Sbjct: 481 VSGQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAYIG 512

BLAST of Lcy03g015720 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.7e-187
Identity = 321/513 (62.57%), Postives = 396/513 (77.19%), Query Frame = 0

Query: 14  TLASAVLLHRFLFL--------RRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 73
           TL   +LL   LFL        R  S + RLPPGP PWPI+GNLP +G  P H  L+AM 
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62

Query: 74  GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 133
             YGP+++LR G VDVVVAAS +VAEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPY
Sbjct: 63  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122

Query: 134 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTICTANA 193
           G RWRLLRKIS+VHLFS+KAL+D + VR+EEV  L + L   G  P  LG L+ +C  NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182

Query: 194 LGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEM 253
           LGR MIGRR+FG  +   D K D+F+++V ++M L+G FN GDF PSL+WLDL GVAG+M
Sbjct: 183 LGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242

Query: 254 KKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDTEIKAL 313
           K++HK FD FL+ IL EH          +H D+LS L+S K    + +   L+DTEIKAL
Sbjct: 243 KRLHKRFDAFLSSILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKAL 302

Query: 314 LLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQA 373
           LLNMFTAGTDTSASTV+WA+AELIRHP ++ + Q+ELD VVGRDR V++ D+ +L YLQA
Sbjct: 303 LLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQA 362

Query: 374 VVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPN 433
           V+KE FRLHPPTPLSLP +A+ESCEING+HIPKGSTLL ++WAI RDP +W+DPL F+P 
Sbjct: 363 VIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPE 422

Query: 434 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLS 493
           RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+SLGLRT+Q LTATLV  FDW L  G++
Sbjct: 423 RFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVT 482

Query: 494 PDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 518
           P+KLNM+E++GLTL+RA PL +HP+PRL+P VY
Sbjct: 483 PEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of Lcy03g015720 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 2.7e-174
Identity = 307/507 (60.55%), Postives = 386/507 (76.13%), Query Frame = 0

Query: 14  TLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMAGEYGPLMY 73
           TL  +  +   L+ RRS+Q   LPPGP  WPI+GNLPQ+G  P H  L A++ +YGPL  
Sbjct: 11  TLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDALSKQYGPLFR 70

Query: 74  LRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLR 133
           LR GSV+VVVA+S+AVA QFL+  DVNF +RPPN+GA+++AYNYQD+VFAPYGPRWR+LR
Sbjct: 71  LRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLR 130

Query: 134 KISAVHLFSSKALDDHRRVREEEVAVLVQTL---ASAGKAPATLGSLLTICTANALGRTM 193
           K+ +VHLFS KALDD R VR+ EVA LV+ L   A  G     LG  L +C  NAL R M
Sbjct: 131 KLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTG-VLVNLGKALNVCATNALARAM 190

Query: 194 IGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHK 253
           +GRRVF D  D    + D+FK +V ++M L+G FN GDF P L WLDL GV G+MK++H+
Sbjct: 191 LGRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHR 250

Query: 254 WFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEIKALLLNMFT 313
            +D FL ++++E++        ++  DLLS+L+  K+   +   KL++T+IKALLLN+FT
Sbjct: 251 RYDAFLDRVIEENQ------ANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLLNLFT 310

Query: 314 AGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETF 373
           AGTDTS+STVEW LAELIRHP +L +TQ ELDSV+GRDRLV++ DLP L YLQAVVKETF
Sbjct: 311 AGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKETF 370

Query: 374 RLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGG 433
           RLHP TPLSLPRMA+E C +NG+ IPK +TLLV+VW+IGRD   W DPLEFRP+RFLPGG
Sbjct: 371 RLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLPGG 430

Query: 434 EKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNM 493
           E+ HVDVKGNDFEVIPFGAGRRIC G+SLGLR VQ +TAT+VH++DW+LP G    KL+M
Sbjct: 431 EREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLDM 490

Query: 494 DEAFGLTLRRAQPLALHPRPRLSPEVY 518
           +EA+GLTL+RA PL + P PRLS + Y
Sbjct: 491 EEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of Lcy03g015720 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 4.3e-164
Identity = 297/533 (55.72%), Postives = 383/533 (71.86%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSS------QSLRLPPGPKPWPIVGNLPQIGG 60
           MD + L +   ++ +++AV  +   FLR  S      +   LPPGP+ WP++GNLPQ+G 
Sbjct: 1   MDVVPLPLLLGSLAVSAAV-WYLVYFLRGGSGGDAARKRRPLPPGPRGWPVLGNLPQLGD 60

Query: 61  APLHSHLAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIA 120
            P H+ + A+A +YGPL  LRFG  +VVVAASA VA QFL+  D NF +RPPN+GA+++A
Sbjct: 61  KPHHT-MCALARQYGPLFRLRFGCAEVVVAASAPVAAQFLRGHDANFSNRPPNSGAEHVA 120

Query: 121 YNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATL 180
           YNYQD+VFAPYG RWR LRK+ A+HLFS+KALDD R VRE EVA++V+ LA    A   L
Sbjct: 121 YNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREGEVALMVRNLARQQAASVAL 180

Query: 181 GSLLTICTANALGRTMIGRRVFG-DGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSL 240
           G    +C  N L R  IG RVF  DG +G      +FK +V ++M L+G FN GDF P+L
Sbjct: 181 GQEANVCATNTLARATIGHRVFAVDGGEG----AREFKEMVVELMQLAGVFNVGDFVPAL 240

Query: 241 EWLDLLGVAGEMKKVHKWFDDFLTKILDEHRNLLAG------AGGSQHQDLLSMLLS--- 300
            WLD  GV  +MK++H+ +D+ +   ++E +   AG      A G    DLLS+LL+   
Sbjct: 241 RWLDPQGVVAKMKRLHRRYDNMMNGFINERK---AGAQPDGVAAGEHGNDLLSVLLARMQ 300

Query: 301 SKDQAEDENEKLSDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSV 360
            + + + + EK+++T+IKALLLN+FTAGTDT++STVEWALAELIRHP +L + Q ELD+V
Sbjct: 301 EEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELIRHPDVLKEAQHELDTV 360

Query: 361 VGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVD 420
           VGR RLVS+ DLPRL YL AV+KETFRLHP TPLSLPR AAE CE++G+ IPKG+TLLV+
Sbjct: 361 VGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEECEVDGYRIPKGATLLVN 420

Query: 421 VWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTV 480
           VWAI RDP +W DPL+++P+RFLPG   A VDVKG DF +IPFGAGRRIC G+S GLR V
Sbjct: 421 VWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGLIPFGAGRRICAGLSWGLRMV 480

Query: 481 QMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 518
            ++TATLVH FDW L  G +PDKLNM+EA+GLTL+RA PL + P PRL P  Y
Sbjct: 481 TLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPLMVQPVPRLLPSAY 524

BLAST of Lcy03g015720 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 6.9e-162
Identity = 284/527 (53.89%), Postives = 385/527 (73.06%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           M  + +I+  +A+   S    + F   +RS+  L+LPPGPK +P++GNLPQ+G  P H  
Sbjct: 3   MTSLDIILFISAIVFLSIYYYNLFSNAKRSN-GLKLPPGPKGYPVLGNLPQLGAKP-HQA 62

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           L A +  YGPLM LR GSVD+VVA+S +VA QFLK  D NF +RPPN+GA+++A+NY D+
Sbjct: 63  LQAFSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDL 122

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTL--ASAGKAPATLGSLL 180
           VFAPYGPRWRLLRK+SAVHL   KALDD++ VREEE+AVL + L   S G  P  +G  +
Sbjct: 123 VFAPYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGEPVNVGKEM 182

Query: 181 TICTANALGRTMIGRRVFGDGSDGGD--------PKVDQFKAIVEQVMVLSGKFNPGDFF 240
            +C+ NAL R M+GRRVF   + GG          K ++FK +V +VM L+G FN GDF 
Sbjct: 183 HVCSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFV 242

Query: 241 PSLEWLDLLGVAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAE 300
           P L+  D+ GV  +MK+VH+ ++ FL K + E R+  A  G +   DLLS+L+  + +++
Sbjct: 243 PWLKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRS-SAKPGAN---DLLSVLIGQRGKSD 302

Query: 301 DENEKLSDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRL 360
               +++DT IKAL+LN+ TAGTDTS+ST+EWAL ELIRHP +L + QQE+DS VGRDRL
Sbjct: 303 GSGGEITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRDRL 362

Query: 361 VSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGR 420
           V++ D+P+L YLQA+VKE FR+HP TPLSLPRM+ E C+I G+HIPK STL V++WA+GR
Sbjct: 363 VTESDVPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAMGR 422

Query: 421 DPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTAT 480
           DP  W DP+EFRP+RFLPGG+  H++V+GN FE++PFGAGRRIC G S+G+R V    AT
Sbjct: 423 DPSIWPDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTVAT 482

Query: 481 LVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 518
           L+H+FDW LP GL+ +K++M+EAFG++L++A PL  HP PRL+P+ Y
Sbjct: 483 LIHAFDWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of Lcy03g015720 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.5e-263
Identity = 451/518 (87.07%), Postives = 483/518 (93.24%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS 
Sbjct: 1   MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VAGEMKKVHKWFDD ++KIL+EHRNLLA  GG +HQDLLS LLS++D+A+DENEKL+DTE
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDSVVGRDR+VSDLDLP+LG
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLG 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEI+G+HIPKGSTLLVDVWAI RDPKEW  PLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQMLTA+LVHSFDWALP
Sbjct: 421 FRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
            GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 481 SGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of Lcy03g015720 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.2e-259
Identity = 448/518 (86.49%), Postives = 479/518 (92.47%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLA+ VLL+R LF +R+S+SL LPPGPKPWP VGNLPQIGGAPLHS 
Sbjct: 1   MDEISLFF-SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LAAM+GEYGPLMYLRFGS+DVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+D+R VREEEV VLVQ LA  G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VA EMKKVHKWFDD L+KIL+EHR LL  A G +HQDLLS LLS++D AEDENEKL+DTE
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLL--ASGGEHQDLLSTLLSTRDHAEDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDSVVGRDR+VSDLDLP+LG
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLG 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEING+HIPKGSTLLVDVWAI RDPKEW  PLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQMLTA+LVHSFDWALP
Sbjct: 421 FRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
            GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 481 SGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of Lcy03g015720 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.5e-249
Identity = 433/525 (82.48%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           M+EI+LIV AA +TLASA LLHR  FLRR     RLPPGPKPWPIVGN+PQIGGAPLHS 
Sbjct: 1   MEEITLIV-AAIITLASAFLLHRTFFLRR---RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LAAMAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LAAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKALDDHRR+R++E++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           C AN+LGRTMIGRRVFGDGS   D + +QFKA+VEQVMVL+GKFNPGDFFP LE LDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA----GAGGSQHQDLLSMLLSSKDQAEDENEKL 300
           V  EMKKVHKWFDDFLTKI++EHRNLLA    G+GG  HQDLLS LLS KD+  DENEKL
Sbjct: 241 VGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKL 300

Query: 301 SDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDL 360
           +DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+M+AQ QQELDSVVGRDR VSDLDL
Sbjct: 301 NDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDL 360

Query: 361 PRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWA 420
           P+L YLQAVVKETFRLHPPTPLSLPRMA++SCE+NG+HIPKGSTLLVDVWAIGRDPKEW 
Sbjct: 361 PQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWV 420

Query: 421 DPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFD 480
           DPLEF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+SLGLR VQMLTAT+VHSFD
Sbjct: 421 DPLEFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFD 480

Query: 481 WALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQGLP 522
           WALP GL+PDKLNMDE +GLTLRRAQPL +HPRPRL   VYQ LP
Sbjct: 481 WALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of Lcy03g015720 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.5e-249
Identity = 433/525 (82.48%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           M+EI+LIV AA +TLASA LLHR  FLRR     RLPPGPKPWPIVGN+PQIGGAPLHS 
Sbjct: 1   MEEITLIV-AAIITLASAFLLHRTFFLRR---RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LAAMAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LAAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKALDDHRR+R++E++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           C AN+LGRTMIGRRVFGDGS   D + +QFKA+VEQVMVL+GKFNPGDFFP LE LDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA----GAGGSQHQDLLSMLLSSKDQAEDENEKL 300
           V  EMKKVHKWFDDFLTKI++EHRNLLA    G+GG  HQDLLS LLS KD+  DENEKL
Sbjct: 241 VGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKL 300

Query: 301 SDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDL 360
           +DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+M+AQ QQELDSVVGRDR VSDLDL
Sbjct: 301 NDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDL 360

Query: 361 PRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWA 420
           P+L YLQAVVKETFRLHPPTPLSLPRMA++SCE+NG+HIPKGSTLLVDVWAIGRDPKEW 
Sbjct: 361 PQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWV 420

Query: 421 DPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFD 480
           DPLEF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+SLGLR VQMLTAT+VHSFD
Sbjct: 421 DPLEFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFD 480

Query: 481 WALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQGLP 522
           WALP GL+PDKLNMDE +GLTLRRAQPL +HPRPRL   VYQ LP
Sbjct: 481 WALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of Lcy03g015720 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.9e-219
Identity = 388/510 (76.08%), Postives = 426/510 (83.53%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           M+EI+ IV AA +TLASA LLHR  FLRR    LRLPPGPKPWPIVGN+PQIG A LHS 
Sbjct: 1   MEEITPIV-AALITLASAFLLHRAFFLRR---RLRLPPGPKPWPIVGNMPQIGEAHLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LA MAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKIS VHLFSSKALDDHRR+REEE++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           C AN+LGRTMIGRRVFGDGS   D + +QFK +VEQVMVL+GKFNPGDFFP LEWLDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           V  EMKKVHKWFDDFLTKI++EHRNLLA   G  HQDLLS LLS KD  EDENEKL+DTE
Sbjct: 241 VGREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLL +F                           ++  L+  +GRDR VSD+DL +L 
Sbjct: 301 IKALLLVLFL--------------------------SKFYLNCFIGRDRAVSDVDLHQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMA++SCE+NG+HIPKGSTLLVDVWAIGRDPK+W DPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRPNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+SLGLR VQMLTAT+VHSFDW LP
Sbjct: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRP 511
            GL+PDKLNMDE +GLTLRRAQPL +HPRP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRP 480

BLAST of Lcy03g015720 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 451/518 (87.07%), Postives = 483/518 (93.24%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS 
Sbjct: 1   MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VAGEMKKVHKWFDD ++KIL+EHRNLLA  GG +HQDLLS LLS++D+A+DENEKL+DTE
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDSVVGRDR+VSDLDLP+LG
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLG 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEI+G+HIPKGSTLLVDVWAI RDPKEW  PLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQMLTA+LVHSFDWALP
Sbjct: 421 FRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
            GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 481 SGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of Lcy03g015720 vs. NCBI nr
Match: KAG6587576.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 914.1 bits (2361), Expect = 5.6e-262
Identity = 450/518 (86.87%), Postives = 481/518 (92.86%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS 
Sbjct: 92  MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 151

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 152 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 211

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA  G+APATLGSLLT+
Sbjct: 212 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARPGEAPATLGSLLTV 271

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPGDFFP LEWLDLLG
Sbjct: 272 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPWLEWLDLLG 331

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VA EMKKVHKWFDD ++KIL+EHRNLLA  GG +HQDLL+ LLS++D A+DENEKL+DTE
Sbjct: 332 VAREMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLTTLLSTRDHAKDENEKLNDTE 391

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDSVVGRDR+VSDLDLP+LG
Sbjct: 392 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLG 451

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEI+G+HIPKGSTLLVDVWAI RDPKEW  PLE
Sbjct: 452 YLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLE 511

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQMLTA+LVHSFDWALP
Sbjct: 512 FRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALP 571

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
            GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 572 SGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 608

BLAST of Lcy03g015720 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.9 bits (2358), Expect = 1.3e-261
Identity = 451/521 (86.56%), Postives = 484/521 (92.90%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS 
Sbjct: 1   MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPGDFFPSL+WLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA---GAGGSQHQDLLSMLLSSKDQAEDENEKLS 300
           VA EMKKVHKWFDD L+KIL+EHRNLLA   G GG +HQDLLS LLS++D+AEDENEKL+
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLN 300

Query: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLP 360
           DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDS+VGRDR+VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLP 360

Query: 361 RLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWAD 420
           +LGYLQAVVKETFRLHPPTPLSLPRMAAESCEI+G+ IPKGSTLLVDVWAI RDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDW 480
           PLEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQ+LTA+LVHSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDW 480

Query: 481 ALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
           ALP GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of Lcy03g015720 vs. NCBI nr
Match: XP_023007386.1 (flavonoid 3'-monooxygenase [Cucurbita maxima])

HSP 1 Score: 904.4 bits (2336), Expect = 4.5e-259
Identity = 448/518 (86.49%), Postives = 479/518 (92.47%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEISL   +AA+TLA+ VLL+R LF +R+S+SL LPPGPKPWP VGNLPQIGGAPLHS 
Sbjct: 1   MDEISLFF-SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LAAM+GEYGPLMYLRFGS+DVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKISAVHLFSSKAL+D+R VREEEV VLVQ LA  G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDGS GGDP  DQFKA+VEQVM+LSGKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VA EMKKVHKWFDD L+KIL+EHR LL  A G +HQDLLS LLS++D AEDENEKL+DTE
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLL--ASGGEHQDLLSTLLSTRDHAEDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWALAELIRHPKM+ Q QQELDSVVGRDR+VSDLDLP+LG
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLG 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEING+HIPKGSTLLVDVWAI RDPKEW  PLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMS+GLR VQMLTA+LVHSFDWALP
Sbjct: 421 FRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQ 519
            GLSP +LNMDEAFGLTLRRAQPL  HPRPRLSP VYQ
Sbjct: 481 SGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of Lcy03g015720 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 445/521 (85.41%), Postives = 471/521 (90.40%), Query Frame = 0

Query: 1   MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
           MDEI+LIV AA VTLAS +LLHR  F RRS    RLPPGPKPWPIVGN+PQIGGAPLHS 
Sbjct: 1   MDEIALIV-AAVVTLASTILLHR-TFFRRS--RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
           LAAMAGEYG LMYLR GSV VVVAASAAVAE+ LK+ D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61  LAAMAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
           VFAPYGPRWRLLRKIS VHLFSSKALDDHRR+REEE+AVLVQ LAS+G+ PA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTV 180

Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
           CTANALGRTMIGRRVFGDG  G D +  QFKA+VEQVM+L+GKFNPGDFFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLG 240

Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
           VA EMKKVHKWFDDFLTKI++EHRNLLA   G  HQDLLS LLSS   AEDENEK++DTE
Sbjct: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLARGRGGGHQDLLSTLLSS---AEDENEKINDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 360
           IKALLLNMFTAGTDTSASTVEWAL ELIRHPKM+AQ QQELDSVVGRDR VSDLDLP+L 
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 420
           YLQAVVKETFRLHPPTPLSLPRMAAESCEING+HIPKG+TLLVDVWAIGRDPKEWADPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLE 420

Query: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 480
           FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMS+GLR VQMLTATLVH FDWALP
Sbjct: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALP 480

Query: 481 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVYQGLP 522
            GL PD+LNMDEA+GLTLRRAQPL +HPRPRL P VYQ  P
Sbjct: 481 NGLLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514

BLAST of Lcy03g015720 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 655.6 bits (1690), Expect = 3.4e-188
Identity = 321/513 (62.57%), Postives = 396/513 (77.19%), Query Frame = 0

Query: 14  TLASAVLLHRFLFL--------RRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 73
           TL   +LL   LFL        R  S + RLPPGP PWPI+GNLP +G  P H  L+AM 
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62

Query: 74  GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 133
             YGP+++LR G VDVVVAAS +VAEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPY
Sbjct: 63  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122

Query: 134 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTICTANA 193
           G RWRLLRKIS+VHLFS+KAL+D + VR+EEV  L + L   G  P  LG L+ +C  NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182

Query: 194 LGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEM 253
           LGR MIGRR+FG  +   D K D+F+++V ++M L+G FN GDF PSL+WLDL GVAG+M
Sbjct: 183 LGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242

Query: 254 KKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDTEIKAL 313
           K++HK FD FL+ IL EH          +H D+LS L+S K    + +   L+DTEIKAL
Sbjct: 243 KRLHKRFDAFLSSILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKAL 302

Query: 314 LLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQA 373
           LLNMFTAGTDTSASTV+WA+AELIRHP ++ + Q+ELD VVGRDR V++ D+ +L YLQA
Sbjct: 303 LLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQA 362

Query: 374 VVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPN 433
           V+KE FRLHPPTPLSLP +A+ESCEING+HIPKGSTLL ++WAI RDP +W+DPL F+P 
Sbjct: 363 VIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPE 422

Query: 434 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLS 493
           RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+SLGLRT+Q LTATLV  FDW L  G++
Sbjct: 423 RFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVT 482

Query: 494 PDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 518
           P+KLNM+E++GLTL+RA PL +HP+PRL+P VY
Sbjct: 483 PEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of Lcy03g015720 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 343.2 bits (879), Expect = 3.7e-94
Identity = 193/517 (37.33%), Postives = 291/517 (56.29%), Query Frame = 0

Query: 6   LIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 65
           L++A+    L   VLL R+L    + ++ RLPPGP   PI+GNL Q+G  P H  LA++ 
Sbjct: 3   LVLASLFAVLILNVLLWRWL-KASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASLC 62

Query: 66  GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 125
            +YGPL+YLR G+VD +         + L  +D  F SRP    A ++AY   D+  AP 
Sbjct: 63  DKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAPM 122

Query: 126 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTL---ASAGKAPATLGSLLTICT 185
           GP W+ +R+I   HL ++K L+     R EE   L++ +   +  GK P  L  +L   +
Sbjct: 123 GPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGK-PINLKEVLGAFS 182

Query: 186 ANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVA 245
            N + R ++G++ FG GS     +  +F  I  ++  L G    GD+ P   W+D  G  
Sbjct: 183 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 242

Query: 246 GEMKKVHKWFDDFLTKILDEHRNL-LAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEI 305
            EM+ V K  D+F TKI+DEHR   L     +   D + +LLS     E+    + D EI
Sbjct: 243 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL--PGENGKAHMEDVEI 302

Query: 306 KALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGY 365
           KAL+ +M  A TDTSA T EWA+AE I+ P+++ + Q+ELD+VVG +R+V + DL  L Y
Sbjct: 303 KALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNY 362

Query: 366 LQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEF 425
           L+ VV+ETFR+HP  P  +P  +  +  ING++IP  + + ++   +GR+ K W D  +F
Sbjct: 363 LRCVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDF 422

Query: 426 RPNRFLPGGEKAHVDVK-GNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 485
           RP R  P      V++  G DF+++PF AG+R C G  LG+  V M  A L H F+W+  
Sbjct: 423 RPERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWS-- 482

Query: 486 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 518
              SP  ++  E +G+T+ +A+PL    +PRL+  +Y
Sbjct: 483 ---SPGNIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 509

BLAST of Lcy03g015720 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-92
Identity = 190/515 (36.89%), Postives = 289/515 (56.12%), Query Frame = 0

Query: 11  AAVTLASAVLLH--RFLFLRRSSQSLR---LPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 70
           ++V LA A LL    +L+++  S+S +   LPPGP P PIVGNLP +    LH+   A+ 
Sbjct: 23  SSVMLAVAALLAVVCYLWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALT 82

Query: 71  GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 130
            +YGPLM +  GS   +V +S  +A + LK  D+ F +       K   Y  +D++++PY
Sbjct: 83  LKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPY 142

Query: 131 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGK--APATLGSLLTICTA 190
           G  WR LRK+  + +F++ +L+     R EE    +  ++   +  +P  LG  + +   
Sbjct: 143 GTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREGSPVNLGEQIFLSIF 202

Query: 191 NALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAG 250
           N + R M G  V GD         ++ K ++  +  + G  N  DFFP     D  G+  
Sbjct: 203 NVVTRMMWGATVEGDERTS---LGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVK 262

Query: 251 EMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEIKA 310
           +MK   K  D    ++++ H  ++ G    + +D L  LL  KD  +DE   LS T +K+
Sbjct: 263 QMKGHVKKLDLLFDRVMESHVKMV-GKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHVKS 322

Query: 311 LLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQ 370
           LL++M   G DTS +  E+A+AE++  P++L + + ELD VVG+D +V +  LP+L YLQ
Sbjct: 323 LLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQ 382

Query: 371 AVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRP 430
           AV+KET RLHP  PL +P   +E+  + G+ +PK S + ++VWAI RDPK W +P EF+P
Sbjct: 383 AVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKP 442

Query: 431 NRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGL 490
            RFL       +D  G DF+ +PFG+GRRIC  +++  R V    A+L+HSFDW  P G 
Sbjct: 443 ERFLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQG- 502

Query: 491 SPDKLNMDEAFGLTLRRAQPLALHPRPRLS-PEVY 518
              K  ++E FGL L+   PL   P PRLS P++Y
Sbjct: 503 --QKFEVEEKFGLVLKLKSPLVAIPVPRLSDPKLY 524

BLAST of Lcy03g015720 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 336.7 bits (862), Expect = 3.5e-92
Identity = 183/499 (36.67%), Postives = 285/499 (57.11%), Query Frame = 0

Query: 20  LLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMAGEYGPLMYLRFGSV 79
           LL   L L++  Q  R PP P  +PI+GNL Q+G  P H  L +++ +YGP+M L+FGS+
Sbjct: 15  LLLAALRLKKRRQHQRKPPSPPGFPIIGNLHQLGELP-HQSLWSLSKKYGPVMLLKFGSI 74

Query: 80  DVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVH 139
             VV +S+  A+Q LKI D+N  SRP  AG + ++YNY D+VF+P+   W+ LR++    
Sbjct: 75  PTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKELRRMCVQE 134

Query: 140 LFSSKALDDHRRVREEEVAVLVQTL--ASAGKAPATLGSLLTICTANALGRTMIGRRVFG 199
           LFS K +   + +REEEV  L+ +   ++A K P  L   L   T       +I +  FG
Sbjct: 135 LFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVG-----VICKAAFG 194

Query: 200 DGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLD--LLGVAGEMKKVHKWFDDF 259
               G     D F  ++    +  G F+  D+FP++ W+   L G+ G+ ++  +  D F
Sbjct: 195 VSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGLDAF 254

Query: 260 LTKILDEHRNLLAGAGGSQHQDLLSMLLS-SKDQAEDENEKLSDTEIKALLLNMFTAGTD 319
             ++ D H+           +D + +LL   K++      KL+   IKA+L+N+   G  
Sbjct: 255 YEQMFDLHKQ----GNKEGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLGGIG 314

Query: 320 TSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHP 379
           TSA T+ WA+ EL+R+P+++ + Q E+ + +G   ++   D+ +L YL+ V+ ET+RLHP
Sbjct: 315 TSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWRLHP 374

Query: 380 PTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAH 439
           P PL +PR      EING+ IP  + L V+VW IGRDP  W DP EF P RF+     ++
Sbjct: 375 PAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFV----NSN 434

Query: 440 VDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAF 499
           +D KG +FE++PFG+GRR+C  M +G   V+   A L++ FDW LP G+  + ++M+E+ 
Sbjct: 435 IDAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESP 494

Query: 500 GLTLRRAQPLALHPRPRLS 514
           GL   +   L L PR  L+
Sbjct: 495 GLNASKKNELVLVPRKYLN 499

BLAST of Lcy03g015720 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 335.5 bits (859), Expect = 7.7e-92
Identity = 187/513 (36.45%), Postives = 288/513 (56.14%), Query Frame = 0

Query: 4   ISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAA 63
           ISL   A AV + +A     +   +RS Q   LPPGP+  PIVGNLP +    LH++   
Sbjct: 13  ISLTPYAYAVLILTATFSILWYIFKRSPQP-PLPPGPRGLPIVGNLPFL-DPDLHTYFTK 72

Query: 64  MAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFA 123
           +A  +GP+  L  GS   VV  S ++A + LK +D+NF +      A+ + Y   D+V+ 
Sbjct: 73  LAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWL 132

Query: 124 PYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAG--KAPATLGSLLTIC 183
           PYG  WR+LRK+ A  LFS K LD    +R +E+    + L   G  K+P  +G  L + 
Sbjct: 133 PYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLT 192

Query: 184 TANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGV 243
             N +   + G  V  +  +       +FK ++ ++  L G  N  DFFP L   DL G+
Sbjct: 193 MMNLMMNMLWGGSVKAEDMESVG---TEFKGVISEITRLLGVPNVSDFFPMLARFDLQGL 252

Query: 244 AGEMKKVHKWFDDFLTKILDEHRNLLAGAG-GSQHQDLLSMLLSSKDQAEDENEKLSDTE 303
             +M    +  D  L + +++ + L +  G   + +D L  L+  +DQ  D +  ++   
Sbjct: 253 VKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNH 312

Query: 304 IKALLLNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLG 363
           +KA+L++M   GT++S +T+E+ +AELI +P+++ + QQELD VVG+D +V +  +  L 
Sbjct: 313 VKAVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLP 372

Query: 364 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLE 423
           Y+ AV+KET RL+P  PL +P   +E+  + G+ IPK + + ++VW+I RDP  W  P E
Sbjct: 373 YILAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTE 432

Query: 424 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALP 483
           FRP RFL   +K   D  G D+  +PFG+GRRIC G++L  R +    ATL+HSFDW +P
Sbjct: 433 FRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIP 492

Query: 484 IGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLS 514
            G     L+++E FG+ L+   PL   P PRLS
Sbjct: 493 DG---HVLDLEEKFGIVLKLKTPLVALPIPRLS 514