Lcy03g014250 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g014250
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCRAL-TRIO domain-containing protein C3H8.02
LocationChr03: 48337034 .. 48340933 (+)
RNA-Seq ExpressionLcy03g014250
SyntenyLcy03g014250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTACGTCCCAGCCTCACTCTACGCCACCCGCTTCTCCCCACCAGTTATCCAATTCCAGCCAGAGCTACTCAATTCCGCAGATCCGCAGTTCAGAATTGCGTCTCCGACTCCGGTGAGTCGCGCAAGGTACTAAGCTTCCACTCCAATATCTACCGATTCTAGTTCGCGTCTGGTTCACTTTGGCTGCCGAGAGATAGCGTTTTGCCATGAGAATGCGATATTGAAGTGTTGTATCATGTATGTTTGGATTCAAGTCAGTTCTATAAACTTCTTTAGGTTGATGTGTCTGTAATTTGTTGAAACTTGTCTTCCTCTTACCACGGAGTAAACGGTTTGCCTGAAAGTTTAGCTGAAATGAGGAGCGGAAATGCTAGCATGTGAGCGTATCTCCCTTTTCTTTAAAGGAGAAGTGTGCTTACTGGCTGTCAATCGATCAATATGCAGCCATTATTCTATACGAGCTTTTTGCACAATATAATGGGGAGTTATTTGAAGATTGAGTGGTTTTATGTTTGATGACCGCGTTGGCTGTATGTATATATTTATATGATGATAAACTGGATAGTTCATGGTTCATGTTTTCAAGTGTTGCAGCTCGTCTTGGAAGTGAAGGAGAAACTTGAAAGAGAATATTCTAGTCTTCCTCTAGGTCGGAATGGAAGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGACCGAAAGTTTTCTGTTGAAGATGCTGTTGCAAAATTAACGAAAGCTATTGTATACACTTGAAACCTCTCTTTTTTATTTTTATTTATAAAACAGTATGTTTCTTTTCAAGTGGCGTTGCAAAAACTGAATTTGCTGTATTAGTTGTTTTTGAAATAAGTTTTACATGTATTGAAAATTTTAGTTAATGTGAGGAATGATGATGCCATAGCATTTATTACATAATTTTATGCATTTCATTTTCCCTTCTGTCTCAAACAGAACTGGCGTCAGGAATTTGGAGTAGATGAACTGTCTGAAGATTCAGTAAAAGAAATAGCTGGAACTGGGAAAGCACTCGTCCATGAGTTTCTTGATGTCAATGACAGGCCAGTACTCATAGTTGTGGCTGCCAAGCATTTACCTGCAGTAAGTTTCATGGTTACTCATTGATATTTGACAGATTTTCTTGGTTTGAGTTAATTGCCCAATCCCATCACTATCCACAGGTCATTTGCAGTACAATTGTCTTCCTTTGTAATTGAATGTTTACTCCATTGTAATTTGAAAGCTCATGCGTTTAGCTTATAACTATCCACGAATTGATGATGCACCTACAACTGCCATTTGTAGTACAGTTGTCTTCCTTGTAATTGAAGATTCATTCCTCTGTCATTGTAAACTTGAAAGCATATGCATTGTAACTTATAACTGTCAATGAATTGAGGATGCACCTACTCCTCCATGAATGGTGAAGTTTGACCTTGTCACGTGCACTATGGCCTCTGTCCTTCCTCAGTGGGCACTATTCCTTATTTTTTATAGTAAAAGAATATGTTTTCGTCTATAATCTCATTTTAATTTTCCCTCTAAACATGAATCTGACTTTACTAATAACTTGACCGCAGGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGACAAGAAGAAATACTTGGAATAATTGATCTGCGAGGTTTTGGTACTGAGAATGCAGATCTTAGGTTCTTGACATTTTTGGTAATTTTACAAGCTGAAATTTATCATTTTCAGTACATAATGGGAACTCAAAAACAATATTTGCAAGGTTGAGATGAGAGATTCATATCTTAACCACTTGGCACTGGAAGAACTATACACACATTGTACGGTTTTTAACATCATAAGTTGTTTGCTCACTTTACCAGGAAGCAATCATTTATAAAATAGTTGTCTAGGTCAATCATTGATTTCAATATATGAAAATTATATTGCATTTTCCACAATATAACAATTAGTGGGTATGCTTACCAGAATGCTAGTTTGCAGCTGCAAGGATACTTCATTTTGTAATCAACAGTATAACTTTGAGCATGAAAAATGAGATTTTCCTCCCTTTTCTCCTTTCTCTTTCCCTCCTGAGGCAGATGTTGTTAGGGGTTTGGATGGGGTCGACATTTTTTCATTTTGATGGCTTCTTTGTCATTTGTTGATTGTCCTTGCTGATCTCTTTATGCTCTTTTCCCTGGCAGTTTGATGTGTTTTACTTCTACTATCCAAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCACCTGTGTTCAGACCACTATGGCAACTTACTAAGCCATTATTAAAATCATACGCCACTCTGGTATGCACTTCTATTTTTCAACTGCAAATTCATGCGCCCTTAGTTATAGACCATAAATCTAGGATTCTAGATTGATAAATATATTGTTCGCAAAGTTGATTGGCTAATCTCCCTGTTCAATTCAAATAATAGGATCCCATCACTTGTGTCATATATGGTGTTTGATTTTCACCATTTTTTAAAACCCCAGATCTTTTTTGACGTATGCCAACTCAATAACATAAGAACTTGGTCACCTAAGCTTTATTGAGCTGTTTGGAATCATCGTTGTCTTCAATCCCTTCCCTACATCATAAATTTGCTCGAGTGTTTGTAATAGTATTTGCATCTTTTTCCTTTTTGAAGGTGAGATTTTGTTCCGTAGATACAATCAGAAAGGAATACTTTACTGAAGCTACGCTGCCTGCCATCTTTAAAGACTGAAGAATTTTGCCACTCTCGACAAACAGGTCCAAGTTAGGTAGTATTACTATTAAGACATACACTTGAAAGATTATAAAATTTCACCTGTCATATTTTGGGATAAAAAAGAAAAAGAAGATTCTTCCAAGCACATTTGATATTCACTTTGCTAATTGTTGAGGCTACATTTCTCCAGAGACAAAGGTCAGTTAACGCATATAAGCCCATGCAACACATAAGTTTCTGAGTTAAATATAATTTCCTCATGATTTTCAGAAAACTGACTAGAAAACTGAATTGTGGATTGGTACCTAAGTTGGAATGAGCTTCTTTAATTTATTGATAATAGGAATTCTGCATATCCAGGTTCACTACTTGCTACTTCTGCAGTATGGTGTGGACGTTTTGCTCGAGATAATATTTCATACAAATGAAAAGGGGGTGGAATTCAACTTTTCTCCCCCAAGTTTTATGTTCTGATTGGATACACGAGGAGGGATTCAGAGATTTACAAGTATGAGTTCAAACGTGGCATGCGGAGGTAAGCAATCCCACCTAAAGAGGTTTAAACACATGGTTTTTTATTATATTATCAGGTTGAATTATCACTAAATGCATTTAAGTTTCTTAATGTATTCTAAAGAAAGCAATTGCGTTTGCTGCTCCCTCAAATTATCTAGATTTATTCACTTGCAATTGTGCAAGGACCTCCATCCTTCTTCTCATGTGTTTTGTGGAGATATTATCTGGCTTTCTCCTTGAAAGCATTCCACTGGTAATAAAAACAAATGCCCACAAATCTCTGTAGACATAGTTCACTTTTGCATAACTCAACAATTAAAGACACGAATCTTTGATCAAGAGGTGAGAGGTTCCACCCCTGGAGAAAAAACACAAACACAAAGCTTTGATCAGTTAATATATTATATGTTTGTTTTAAATGATAGATGTTTGAGTCTCCATCTCACATCTGGCTTTCTCCTTGAAAGCATTCCACTGGTAATAAAAACAAATCCCCAGGAATCTTTGATCAAGAGGTGAGAGGTTTGACCCCTGAAGAAAAAACACAAACACAAGCTTTGATCAATTAAGATATTATATGTTTGTTCTAAATGATATATGTTTGAGTCTCCATCTCACAT

mRNA sequence

ATGGCGGTACGTCCCAGCCTCACTCTACGCCACCCGCTTCTCCCCACCAGTTATCCAATTCCAGCCAGAGCTACTCAATTCCGCAGATCCGCAGTTCAGAATTGCGTCTCCGACTCCGGTGAGTCGCGCAAGCTCGTCTTGGAAGTGAAGGAGAAACTTGAAAGAGAATATTCTAGTCTTCCTCTAGGTCGGAATGGAAGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGACCGAAAGTTTTCTGTTGAAGATGCTGTTGCAAAATTAACGAAAGCTATTAACTGGCGTCAGGAATTTGGAGTAGATGAACTGTCTGAAGATTCAGTAAAAGAAATAGCTGGAACTGGGAAAGCACTCGTCCATGAGTTTCTTGATGTCAATGACAGGCCAGTACTCATAGTTGTGGCTGCCAAGCATTTACCTGCAGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGACAAGAAGAAATACTTGGAATAATTGATCTGCGAGGTTTTGGTACTGAGAATGCAGATCTTAGGTTCTTGACATTTTTGTTTGATGTGTTTTACTTCTACTATCCAAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCACCTGTGTTCAGACCACTATGGCAACTTACTAAGCCATTATTAAAATCATACGCCACTCTGGTGAGATTTTGTTCCGTAGATACAATCAGAAAGGAATACTTTACTGAAGCTACGCTGCCTGCCATCTTTAAAGACTGAAGAATTTTGCCACTCTCGACAAACAGGTTCACTACTTGCTACTTCTGCAGTATGGTGTGGACGTTTTGCTCGAGATAATATTTCATACAAATGAAAAGGGGGTGGAATTCAACTTTTCTCCCCCAAGTTTTATGTTCTGATTGGATACACGAGGAGGGATTCAGAGATTTACAAGTATGAGTTCAAACGTGGCATGCGGAGGTAAGCAATCCCACCTAAAGAGGTTTAAACACATGGTTTTTTATTATATTATCAGGTTGAATTATCACTAAATGCATTTAAGTTTCTTAATGTATTCTAAAGAAAGCAATTGCGTTTGCTGCTCCCTCAAATTATCTAGATTTATTCACTTGCAATTGTGCAAGGACCTCCATCCTTCTTCTCATGTGTTTTGTGGAGATATTATCTGGCTTTCTCCTTGAAAGCATTCCACTGGTAATAAAAACAAATGCCCACAAATCTCTGTAGACATAGTTCACTTTTGCATAACTCAACAATTAAAGACACGAATCTTTGATCAAGAGGTGAGAGGTTCCACCCCTGGAGAAAAAACACAAACACAAAGCTTTGATCAGTTAATATATTATATGTTTGTTTTAAATGATAGATGTTTGAGTCTCCATCTCACATCTGGCTTTCTCCTTGAAAGCATTCCACTGGTAATAAAAACAAATCCCCAGGAATCTTTGATCAAGAGGTGAGAGGTTTGACCCCTGAAGAAAAAACACAAACACAAGCTTTGATCAATTAAGATATTATATGTTTGTTCTAAATGATATATGTTTGAGTCTCCATCTCACAT

Coding sequence (CDS)

ATGGCGGTACGTCCCAGCCTCACTCTACGCCACCCGCTTCTCCCCACCAGTTATCCAATTCCAGCCAGAGCTACTCAATTCCGCAGATCCGCAGTTCAGAATTGCGTCTCCGACTCCGGTGAGTCGCGCAAGCTCGTCTTGGAAGTGAAGGAGAAACTTGAAAGAGAATATTCTAGTCTTCCTCTAGGTCGGAATGGAAGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGACCGAAAGTTTTCTGTTGAAGATGCTGTTGCAAAATTAACGAAAGCTATTAACTGGCGTCAGGAATTTGGAGTAGATGAACTGTCTGAAGATTCAGTAAAAGAAATAGCTGGAACTGGGAAAGCACTCGTCCATGAGTTTCTTGATGTCAATGACAGGCCAGTACTCATAGTTGTGGCTGCCAAGCATTTACCTGCAGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGACAAGAAGAAATACTTGGAATAATTGATCTGCGAGGTTTTGGTACTGAGAATGCAGATCTTAGGTTCTTGACATTTTTGTTTGATGTGTTTTACTTCTACTATCCAAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCACCTGTGTTCAGACCACTATGGCAACTTACTAAGCCATTATTAAAATCATACGCCACTCTGGTGAGATTTTGTTCCGTAGATACAATCAGAAAGGAATACTTTACTGAAGCTACGCTGCCTGCCATCTTTAAAGACTGA

Protein sequence

MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSLPLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKALVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFCSVDTIRKEYFTEATLPAIFKD
Homology
BLAST of Lcy03g014250 vs. ExPASy Swiss-Prot
Match: Q10138 (CRAL-TRIO domain-containing protein C3H8.02 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC3H8.02 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.9e-13
Identity = 65/240 (27.08%), Postives = 112/240 (46.67%), Query Frame = 0

Query: 37  SDSGESRKLVLEVKEKLEREYSSLPL------GRNGRDDEEMILWFLKDRKFSVEDAVAK 96
           S + +++ + LE  EK+  E+    L        N  D + ++L FL+ RK++VE A+  
Sbjct: 85  SFNDKTKNVHLERVEKIASEWDPEGLRVCFWDAVNCDDPDGLLLRFLRARKWNVEAALEM 144

Query: 97  LTKAINWR-QEFGVDEL---------SEDSVKEIAGTGKALVHEFLDVNDRPVLIVVAAK 156
             K ++WR +E  V E+          +D V+++   GK  +    D ++RPV  + A  
Sbjct: 145 FMKTVHWRSREMNVGEIVCNADHLDKDDDFVRQLR-IGKCFIFG-EDKHNRPVCYIRARL 204

Query: 157 HLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFY 216
           H      P   E+L V+ +E A   L P  E    + D+  F   N D   L F+   F 
Sbjct: 205 HKVGDVSPESVERLTVWVMETARLILKPPIETATVVFDMTDFSMSNMDYGPLKFMIKCFE 264

Query: 217 FYYPKRLGQVLFVEAPPVFRPLWQLTKPLL-----------KSYATLVRFCSVDTIRKEY 250
            +YP+ LG+ +  +AP +F+ +W + K  L           ++Y  L ++ + D I KE+
Sbjct: 265 AHYPECLGECIVHKAPWLFQGVWSIIKSWLDPVVVSKVKFTRNYRDLQQYINPDNILKEF 322

BLAST of Lcy03g014250 vs. ExPASy Swiss-Prot
Match: Q9CWP6 (Motile sperm domain-containing protein 2 OS=Mus musculus OX=10090 GN=Mospd2 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-10
Identity = 54/222 (24.32%), Postives = 108/222 (48.65%), Query Frame = 0

Query: 44  KLVLEVKEKLEREYSSLPLGR-NGRD------DEEMILWFLKDRKFSVEDAVAKLTKAIN 103
           KL+ E + + E EY +    + + RD      D+  +  +L  R   V++ +  L ++  
Sbjct: 11  KLISETRRRFEAEYVTEKSEKYDSRDVERLQQDDNWVESYLYWRHNVVDETLKMLDESFQ 70

Query: 104 WRQEFGVDELSEDSV-KEIAGTGKALVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLC 163
           WR+EF V++LSE S+ + +   G   +H + D     +  +    H+      ++ +KL 
Sbjct: 71  WRKEFSVNDLSESSIPRWLLELGGIYLHGY-DKEGNKLFWIRVKYHIKDQKTIMDKKKLI 130

Query: 164 VFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEA 223
            F++E+   +     + I  + D+   G  + D+ F+ F+ + F  YYPK L +++  + 
Sbjct: 131 AFWLERYAKR--ENGKPITVMFDMSETGLNSIDMDFVRFIINCFKVYYPKYLSKIVIFDM 190

Query: 224 PPVFRPLWQLTKPLLKSYA-TLVRFCSVDTIRKEYFTEATLP 257
           P +    +++ K  L   A +L++F S + I +EY +   LP
Sbjct: 191 PWIMNAAFKIVKSWLGPEAVSLLKFTSKNEI-QEYVSVEYLP 228

BLAST of Lcy03g014250 vs. ExPASy Swiss-Prot
Match: Q757H2 (Phosphatidylinositol transfer protein CSR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CSR1 PE=3 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 1.8e-09
Identity = 48/200 (24.00%), Postives = 84/200 (42.00%), Query Frame = 0

Query: 70  EEMILWFLKDRKFSVEDAVAKLTKAINWR------------QEFGVDELSEDSVKEIAGT 129
           + ++L F++ RK+ +  A+  +  ++ WR             E G  +  +  + +    
Sbjct: 138 DNLLLRFVRARKWDINKAMIMMAHSLRWRLNEGKPEDIVFGGERGAQKADKKGIVKQLEL 197

Query: 130 GKALVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGII 189
           GKA V  F D N  P++ V    H  A     E  +  +  +E+A   L    +    + 
Sbjct: 198 GKATVRGF-DKNGCPIVYVRPRLHHAADQTEAETSEYSLLIIEQARLFLKEPCDTATILF 257

Query: 190 DLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKS-YATL 249
           DL GF   N D   + FL   F  +YP+ LG++   +AP +F P+W + K  L    A  
Sbjct: 258 DLSGFSMANMDYAPVKFLITCFEAHYPECLGKLFIHKAPWIFPPIWNIIKNWLDPVVAAK 317

Query: 250 VRFCSVDTIRKEYFTEATLP 257
           + F       +E+     +P
Sbjct: 318 IAFTKTAADLEEFIPAEQIP 336

BLAST of Lcy03g014250 vs. ExPASy Swiss-Prot
Match: Q06705 (Phosphatidylinositol transfer protein CSR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSR1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.0e-09
Identity = 49/168 (29.17%), Postives = 78/168 (46.43%), Query Frame = 0

Query: 73  ILWFLKDRKFSVEDAVAKLTKAINWRQ----------EFGVDELSEDSVKEIAGTGKALV 132
           IL F++ RK++ +  +A L   + WR+          E  V E +E  V +     KA +
Sbjct: 112 ILKFIRARKWNADKTIAMLGHDLYWRKDTINKIINGGERAVYENNETGVIKNLELQKATI 171

Query: 133 HEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKA---LSKLPPGQEEILGIIDL 192
             + D + RPV++V    H  +     E EK  +  +E++     +  P    IL   DL
Sbjct: 172 QGY-DNDMRPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENYPASTTIL--FDL 231

Query: 193 RGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTK 228
            GF   N D   + FL   F  +YP+ LG +L  +AP +F P+W + K
Sbjct: 232 NGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIK 276

BLAST of Lcy03g014250 vs. ExPASy Swiss-Prot
Match: Q8NHP6 (Motile sperm domain-containing protein 2 OS=Homo sapiens OX=9606 GN=MOSPD2 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.2e-09
Identity = 50/222 (22.52%), Postives = 107/222 (48.20%), Query Frame = 0

Query: 44  KLVLEVKEKLEREYSSLPLGR-NGRD------DEEMILWFLKDRKFSVEDAVAKLTKAIN 103
           KL+ E + + E EY +    + + RD      D+  +  +L  R   V++ +  L ++  
Sbjct: 11  KLISETRRRFEAEYVTDKSDKYDARDVERLQQDDNWVESYLSWRHNIVDETLKMLDESFQ 70

Query: 104 WRQEFGVDELSEDSV-KEIAGTGKALVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLC 163
           WR+E  V++L+E S+ + +   G   +H + D     +  +    H+      ++ +KL 
Sbjct: 71  WRKEISVNDLNESSIPRWLLEIGVIYLHGY-DKEGNKLFWIRVKYHVKDQKTILDKKKLI 130

Query: 164 VFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEA 223
            F++E+   +     + +  + DL   G  + D+ F+ F+ + F  YYPK L +++  + 
Sbjct: 131 AFWLERYAKR--ENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDM 190

Query: 224 PPVFRPLWQLTKPLLKSYA-TLVRFCSVDTIRKEYFTEATLP 257
           P +    +++ K  L   A +L++F S + + ++Y +   LP
Sbjct: 191 PWLMNAAFKIVKTWLGPEAVSLLKFTSKNEV-QDYVSVEYLP 228

BLAST of Lcy03g014250 vs. ExPASy TrEMBL
Match: A0A5A7UWI9 (CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001300 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 241/261 (92.34%), Postives = 251/261 (96.17%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLPTSYPIP RATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +H+FLDVNDRPVL+VVA+KHLP VHDPVEDEKLCVFYVEKALSKLPPG+EEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTI+KEYFTEATLPAIFKD
Sbjct: 241 SVDTIKKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. ExPASy TrEMBL
Match: A0A1S3CLW3 (CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo OX=3656 GN=LOC103502396 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 241/261 (92.34%), Postives = 251/261 (96.17%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLPTSYPIP RATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +H+FLDVNDRPVL+VVA+KHLP VHDPVEDEKLCVFYVEKALSKLPPG+EEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTI+KEYFTEATLPAIFKD
Sbjct: 241 SVDTIKKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. ExPASy TrEMBL
Match: A0A0A0LS15 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004290 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 237/261 (90.80%), Postives = 251/261 (96.17%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLPTSYPIP RATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVED VAKLT+AINWR+EFGVDELSED VKE+A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDTVAKLTRAINWRREFGVDELSEDKVKEMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +H+FLDVNDRPVL+VVA+KHLPA+HDPVEDEKLCVFYVEKALSKLPPG+EEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLLVVASKHLPAIHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSY++LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYSSLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTI+KEYFTEATLPAIFKD
Sbjct: 241 SVDTIKKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. ExPASy TrEMBL
Match: A0A6J1C2T4 (CRAL-TRIO domain-containing protein C3H8.02 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007424 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.7e-130
Identity = 238/261 (91.19%), Postives = 247/261 (94.64%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR  LT RHPLL  SYP+P+RATQFRR AVQNCVSDSGESRKLVLEVKEKLEREYS L
Sbjct: 1   MAVRLILTPRHPLLLASYPVPSRATQFRRFAVQNCVSDSGESRKLVLEVKEKLEREYSDL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAI+WRQEFGVD LSEDSVK IA TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAISWRQEFGVDTLSEDSVKGIAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            VHEFLDVND+PVLIVVA+KHLPAVHDPVEDEKLCVFYVEKALSKLP G+EEILGIIDLR
Sbjct: 121 FVHEFLDVNDKPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPTGKEEILGIIDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GFGTENADL+FLTFLFDVFY YYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+LVRFC
Sbjct: 181 GFGTENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDT+RKEYFTEATLPAIFKD
Sbjct: 241 SVDTVRKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. ExPASy TrEMBL
Match: A0A6J1KM75 (CRAL-TRIO domain-containing protein C3H8.02-like OS=Cucurbita maxima OX=3661 GN=LOC111496517 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.1e-130
Identity = 237/261 (90.80%), Postives = 246/261 (94.25%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MA R SLTLRHP LP SYPIPARATQFRR AVQNCVSDS +SRKLVLEVKEKLEREY+SL
Sbjct: 1   MAARLSLTLRHPFLPASYPIPARATQFRRFAVQNCVSDSYDSRKLVLEVKEKLEREYNSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSV+DAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVDDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            VHEFLDVN RPVLIVVAAKHLPAVHDP+EDEKLCVFYVEKALSKLP G+E+ILGIIDLR
Sbjct: 121 FVHEFLDVNGRPVLIVVAAKHLPAVHDPLEDEKLCVFYVEKALSKLPTGKEDILGIIDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+L RFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLARFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           S+DTIRKEYFTE TLPAIFKD
Sbjct: 241 SIDTIRKEYFTEDTLPAIFKD 261

BLAST of Lcy03g014250 vs. NCBI nr
Match: XP_008464545.1 (PREDICTED: CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo] >KAA0057841.1 CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo var. makuwa] >TYJ98525.1 CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo var. makuwa])

HSP 1 Score: 485.3 bits (1248), Expect = 3.2e-133
Identity = 241/261 (92.34%), Postives = 251/261 (96.17%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLPTSYPIP RATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +H+FLDVNDRPVL+VVA+KHLP VHDPVEDEKLCVFYVEKALSKLPPG+EEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTI+KEYFTEATLPAIFKD
Sbjct: 241 SVDTIKKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. NCBI nr
Match: XP_004138274.1 (CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >XP_011655843.1 CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >XP_011655974.1 CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >XP_031737849.1 CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >XP_031737856.1 CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >KGN63562.1 hypothetical protein Csa_013812 [Cucumis sativus])

HSP 1 Score: 482.6 bits (1241), Expect = 2.1e-132
Identity = 237/261 (90.80%), Postives = 251/261 (96.17%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLPTSYPIP RATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVED VAKLT+AINWR+EFGVDELSED VKE+A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDTVAKLTRAINWRREFGVDELSEDKVKEMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +H+FLDVNDRPVL+VVA+KHLPA+HDPVEDEKLCVFYVEKALSKLPPG+EEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLLVVASKHLPAIHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSY++LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYSSLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTI+KEYFTEATLPAIFKD
Sbjct: 241 SVDTIKKEYFTEATLPAIFKD 261

BLAST of Lcy03g014250 vs. NCBI nr
Match: XP_038878441.1 (CRAL-TRIO domain-containing protein C3H8.02 [Benincasa hispida] >XP_038878442.1 CRAL-TRIO domain-containing protein C3H8.02 [Benincasa hispida])

HSP 1 Score: 478.8 bits (1231), Expect = 3.0e-131
Identity = 240/261 (91.95%), Postives = 250/261 (95.79%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MAVR SLTLRHPLLP+SYPIP RATQFRR AVQNCVSDS  SRKLVLEVKEKLERE+S+L
Sbjct: 1   MAVRLSLTLRHPLLPSSYPIPVRATQFRRFAVQNCVSDSDHSRKLVLEVKEKLEREHSNL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSE SVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEYSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            +HEFLDVNDRPVLIVVA+KHLPAVHDPVEDEKLCVFYVEKAL+KLPPG+EEILGIIDLR
Sbjct: 121 YIHEFLDVNDRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALNKLPPGKEEILGIIDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+LVRFC
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVRFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           SVDTIRKEYFTEATLPAIF+D
Sbjct: 241 SVDTIRKEYFTEATLPAIFED 261

BLAST of Lcy03g014250 vs. NCBI nr
Match: KAG6587463.1 (Motile sperm domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021442.1 Motile sperm domain-containing protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 475.3 bits (1222), Expect = 3.3e-130
Identity = 238/261 (91.19%), Postives = 246/261 (94.25%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MA R SLTLRHP LP SYPIPARATQFRR AVQNCVSDS +SRKLVLEVKEKLEREYSSL
Sbjct: 1   MAARLSLTLRHPFLPASYPIPARATQFRRLAVQNCVSDSYDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSV+DAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVDDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            VHEFLDVN RPVLIVVAAKHLPAVHDP+EDEKLCVFYVEKALSKLP G+E+ILGIIDLR
Sbjct: 121 FVHEFLDVNGRPVLIVVAAKHLPAVHDPLEDEKLCVFYVEKALSKLPTGKEDILGIIDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+L RFC
Sbjct: 181 GFSTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLARFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           S+DTIRKEYFTE TLPAIFKD
Sbjct: 241 SIDTIRKEYFTEDTLPAIFKD 261

BLAST of Lcy03g014250 vs. NCBI nr
Match: XP_023529937.1 (CRAL-TRIO domain-containing protein C3H8.02-like [Cucurbita pepo subsp. pepo] >XP_023529938.1 CRAL-TRIO domain-containing protein C3H8.02-like [Cucurbita pepo subsp. pepo] >XP_023529939.1 CRAL-TRIO domain-containing protein C3H8.02-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 474.2 bits (1219), Expect = 7.4e-130
Identity = 237/261 (90.80%), Postives = 246/261 (94.25%), Query Frame = 0

Query: 1   MAVRPSLTLRHPLLPTSYPIPARATQFRRSAVQNCVSDSGESRKLVLEVKEKLEREYSSL 60
           MA R SLTLRHP LP SYPIPARATQFRR AVQNCVSDS +SRKLVLEVKEKLEREY+SL
Sbjct: 1   MAARLSLTLRHPFLPASYPIPARATQFRRIAVQNCVSDSYDSRKLVLEVKEKLEREYNSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKA 120
           PLGRNGRDDEEMILWFLKDRKFSV+DAVAKLTKAINWRQEFGVDELSEDSVK +A TGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVDDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 LVHEFLDVNDRPVLIVVAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLR 180
            VHEFLDVN RPVLIVVAAKHLPAVHDP+EDEKLCVFYVEKALSKLP G+E+ILGIIDLR
Sbjct: 121 FVHEFLDVNGRPVLIVVAAKHLPAVHDPLEDEKLCVFYVEKALSKLPTGKEDILGIIDLR 180

Query: 181 GFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLKSYATLVRFC 240
           GF TENADLRFLTFLFDVFYFYYPKRLGQVLFVEAP VFRPLWQLTKPLLKSYA+L RFC
Sbjct: 181 GFSTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLARFC 240

Query: 241 SVDTIRKEYFTEATLPAIFKD 262
           S+DTIRKEYFTE TLPAIFKD
Sbjct: 241 SIDTIRKEYFTEDTLPAIFKD 261

BLAST of Lcy03g014250 vs. TAIR 10
Match: AT5G63060.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.0e-93
Identity = 159/232 (68.53%), Postives = 197/232 (84.91%), Query Frame = 0

Query: 29  RSAVQNCVSDSGESRKLVLEVKEKLEREYSSLPLGRNGRDDEEMILWFLKDRKFSVEDAV 88
           R +V++CVS+S  + KLVLEVKE+L ++ +SLPLG+ GRDDE+MILWFLKDR+FSV++A+
Sbjct: 31  RFSVRSCVSESQHAHKLVLEVKERLAKDCTSLPLGKYGRDDEDMILWFLKDRRFSVDEAI 90

Query: 89  AKLTKAINWRQEFGVDELSEDSVKEIAGTGKALVHEFLDVNDRPVLIVVAAKHLPAVHDP 148
            KLTKAI WR EF VDELSEDS+K    TGKA VH FLDV  RPV+IV  AKH+P + DP
Sbjct: 91  GKLTKAIKWRHEFKVDELSEDSIKAATDTGKAYVHGFLDVKGRPVVIVAPAKHIPGLLDP 150

Query: 149 VEDEKLCVFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLG 208
           +EDEKLCVF +EKALSKLP GQ +ILGI DLRGFG++NADL+FLTFLFDVFY+YYP RL 
Sbjct: 151 IEDEKLCVFLLEKALSKLPAGQHKILGIFDLRGFGSQNADLKFLTFLFDVFYYYYPSRLD 210

Query: 209 QVLFVEAPPVFRPLWQLTKPLLKSYATLVRFCSVDTIRKEYFTEATLPAIFK 261
           +VLFV+AP +F+P+WQ TKPL+K YA+LV+FCS +T+RKEYFTE TLP+ F+
Sbjct: 211 EVLFVDAPFIFQPIWQFTKPLVKQYASLVKFCSAETVRKEYFTEETLPSNFR 262

BLAST of Lcy03g014250 vs. TAIR 10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 83.2 bits (204), Expect = 3.4e-16
Identity = 49/182 (26.92%), Postives = 91/182 (50.00%), Query Frame = 0

Query: 51  EKLEREYSSLPLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDS 110
           E++ +    LP   +    ++ +L +L+ R + V+ A   L + + WR ++  +E+  + 
Sbjct: 24  EEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQYKPEEICWEE 83

Query: 111 VKEIAGTGKALVHEFLDVNDRPVLIV-VAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPG 170
           V   A TGK      +D   RPVLI+  + ++  +V   +   +  V+ +E A+  LPPG
Sbjct: 84  VAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQI---RYLVYCMENAVQNLPPG 143

Query: 171 QEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPL 230
           +E+++ +ID  G+   N  LR       V   +YP+RL   +    P  F P W++ +P 
Sbjct: 144 EEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKFFEPFWKVARPF 202

Query: 231 LK 232
           L+
Sbjct: 204 LE 202

BLAST of Lcy03g014250 vs. TAIR 10
Match: AT4G08690.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 83.2 bits (204), Expect = 3.4e-16
Identity = 49/182 (26.92%), Postives = 91/182 (50.00%), Query Frame = 0

Query: 51  EKLEREYSSLPLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDS 110
           E++ +    LP   +    ++ +L +L+ R + V+ A   L + + WR ++  +E+  + 
Sbjct: 24  EEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQYKPEEICWEE 83

Query: 111 VKEIAGTGKALVHEFLDVNDRPVLIV-VAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPG 170
           V   A TGK      +D   RPVLI+  + ++  +V   +   +  V+ +E A+  LPPG
Sbjct: 84  VAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQI---RYLVYCMENAVQNLPPG 143

Query: 171 QEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPL 230
           +E+++ +ID  G+   N  LR       V   +YP+RL   +    P  F P W++ +P 
Sbjct: 144 EEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKFFEPFWKVARPF 202

Query: 231 LK 232
           L+
Sbjct: 204 LE 202

BLAST of Lcy03g014250 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-15
Identity = 60/233 (25.75%), Postives = 103/233 (44.21%), Query Frame = 0

Query: 43  RKLVLEVKEKLEREYSSLPLGRNGRDDEE-------MILWFLKDRKFSVEDAVAKLTKAI 102
           +K V  +++++ER    +      R D E       MI  FL+ R   +E A       +
Sbjct: 16  QKTVPLIEDEIERSKVGIMRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYL 75

Query: 103 NWRQEFGVDELSEDSVKEIAGTGKALVHEFL-----DVNDRPVLIVVAAKHLPAVHDPVE 162
            W++      L +  + E A     L H  +     D   RP+ + +  +H P+  +P E
Sbjct: 76  TWKRSM----LPKGHIPE-AEIANDLSHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDE 135

Query: 163 DEKLCVFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTFLFDVFYFYYPKRLGQV 222
            ++  V+ +EK  +++P GQE+ + I DL+G+G  N D+R            YP+RLG++
Sbjct: 136 FKRFVVYTLEKICARMPRGQEKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKL 195

Query: 223 LFVEAPPVFRPLWQLTKPLL----KSYATLVRFCSVDTIRKEYFTEATLPAIF 260
             V AP +F   W++  P +    K     V    +     E   E+ LP I+
Sbjct: 196 YIVHAPYIFMTAWKVIYPFIDANTKKKIVFVENKKLTPTLLEDIDESQLPDIY 243

BLAST of Lcy03g014250 vs. TAIR 10
Match: AT1G22180.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.7e-11
Identity = 39/157 (24.84%), Postives = 75/157 (47.77%), Query Frame = 0

Query: 76  FLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKEIAGTGKALVHEFLDVNDRPVLI 135
           +L  R   V+ A   L + + WR ++  +E+  + +   A TGK       D   R VL+
Sbjct: 52  YLAARNGHVKKATKMLKETLKWRAQYKPEEIRWEEIAREAETGKIYRANCTDKYGRTVLV 111

Query: 136 V-VAAKHLPAVHDPVEDEKLCVFYVEKALSKLPPGQEEILGIIDLRGFGTENADLRFLTF 195
           +  + ++  +    +   ++ V+ +E A+  LP  QE+++ +ID  GF   +  L+    
Sbjct: 112 MRPSCQNTKSYKGQI---RILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRE 171

Query: 196 LFDVFYFYYPKRLGQVLFVEAPPVFRPLWQLTKPLLK 232
              V   +YP+RLG  +    P +F   +++ KP L+
Sbjct: 172 TAHVLQEHYPERLGLAIVYNPPKIFESFYKMVKPFLE 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q101385.9e-1327.08CRAL-TRIO domain-containing protein C3H8.02 OS=Schizosaccharomyces pombe (strain... [more]
Q9CWP61.6e-1024.32Motile sperm domain-containing protein 2 OS=Mus musculus OX=10090 GN=Mospd2 PE=1... [more]
Q757H21.8e-0924.00Phosphatidylinositol transfer protein CSR1 OS=Ashbya gossypii (strain ATCC 10895... [more]
Q067053.0e-0929.17Phosphatidylinositol transfer protein CSR1 OS=Saccharomyces cerevisiae (strain A... [more]
Q8NHP65.2e-0922.52Motile sperm domain-containing protein 2 OS=Homo sapiens OX=9606 GN=MOSPD2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UWI91.6e-13392.34CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CLW31.6e-13392.34CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo OX=3656 GN=LOC103502... [more]
A0A0A0LS151.0e-13290.80CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004290 P... [more]
A0A6J1C2T44.7e-13091.19CRAL-TRIO domain-containing protein C3H8.02 isoform X1 OS=Momordica charantia OX... [more]
A0A6J1KM756.1e-13090.80CRAL-TRIO domain-containing protein C3H8.02-like OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
XP_008464545.13.2e-13392.34PREDICTED: CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo] >KAA005784... [more]
XP_004138274.12.1e-13290.80CRAL-TRIO domain-containing protein C3H8.02 isoform X1 [Cucumis sativus] >XP_011... [more]
XP_038878441.13.0e-13191.95CRAL-TRIO domain-containing protein C3H8.02 [Benincasa hispida] >XP_038878442.1 ... [more]
KAG6587463.13.3e-13091.19Motile sperm domain-containing protein 2, partial [Cucurbita argyrosperma subsp.... [more]
XP_023529937.17.4e-13090.80CRAL-TRIO domain-containing protein C3H8.02-like [Cucurbita pepo subsp. pepo] >X... [more]
Match NameE-valueIdentityDescription
AT5G63060.12.0e-9368.53Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.23.4e-1626.92Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.13.4e-1626.92Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G01630.12.2e-1525.75Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G22180.25.7e-1124.84Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 114..256
e-value: 4.7E-14
score: 62.7
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 113..250
e-value: 1.4E-19
score: 70.3
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 110..261
score: 12.324567
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 131..232
e-value: 2.07244E-17
score: 75.1142
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 15..250
e-value: 4.6E-38
score: 133.0
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 109..249
NoneNo IPR availablePANTHERPTHR47556SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEINcoord: 18..261
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 40..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g014250.1Lcy03g014250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane