Lcy03g013460 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g013460
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAmino-acid racemase isoform 2
LocationChr03: 47472361 .. 47474191 (-)
RNA-Seq ExpressionLcy03g013460
SyntenyLcy03g013460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTGTCCCACTGACATTCCTCCATTTTTGTCATTCTTCTCTTCAACGGCTGACAGCGCTTCACTGCTAAAAAGGCTGACATATGACAGATTTTCAATAATGTAAGATTCTCTCAAACTCTTCTTTCATTTTGATTTTTGACAATCAATTCAATCACCTCACATCGTGGAGCTTCTTTTCATGTCTTACCTAGTTTTGCTCCACAGTATCTGTCTGCTATGAATTCTCTTCCTTCCAAAACTATCCAACCAACTGTACAATTGCTTTTCGCCTTGAATGTGAAGAAACCCAGAATCATTTCTTCTTTACAACCTTTGTGGCTTATGGCTTGAGATATCATGTCCTTGTTGATATTTTCATCCCGAGAAATATTTGGGTATTGGGGCATGAGTAAAACAGGCTGCTTTTTTATTTGAGTTTCTGAAGAAGTTTGATGCTGGAATTTGGGAGTTGATGAGTCTTCTTTTATGCTTTCCCCATTGAATTATCTGAACTGAAGAGAAATATGGCTGCTTAGTAAGATTCAGAGTTGAATCACACTGAAAATTCTTGCTCTTTACATAAAGGATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCACTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGATATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCCAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTTCCCTCTAACTCCGTCGAGTACATTCAGCGCAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCAAAAAAGGGCGTTTCTCGAACAGTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGATGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGTTTTGTAAATACATTCTCAACTAATGGTCGATACTCTAATTTACTTGATAAAGAGTGATGCACAAGCAATATGAAGCAGCAACACATAACAAAATCTATGCTTTATACTAATCTCTGAGCATCCAGAAAAATGGATAACTTTGCCATGCCTCATAATTAAAATGGATGTTGTTGAAGTGATTGCGGGCATTTTTCTACACTGTTCTGTAGATGATTCCAAACTTTTCTTCTGATTTCCTTTTCTTTCTTCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTTTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

mRNA sequence

ATGCTCTGTCCCACTGACATTCCTCCATTTTTGTCATTCTTCTCTTCAACGGCTGACAGCGCTTCACTGCTAAAAAGGCTGACATATGACAGATTTTCAATAATGATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCACTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGATATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCCAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTTCCCTCTAACTCCGTCGAGTACATTCAGCGCAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCAAAAAAGGGCGTTTCTCGAACAGTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGATGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTTTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

Coding sequence (CDS)

ATGCTCTGTCCCACTGACATTCCTCCATTTTTGTCATTCTTCTCTTCAACGGCTGACAGCGCTTCACTGCTAAAAAGGCTGACATATGACAGATTTTCAATAATGATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCACTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGATATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCCAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTTCCCTCTAACTCCGTCGAGTACATTCAGCGCAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCAAAAAAGGGCGTTTCTCGAACAGTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGATGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTTTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

Protein sequence

MLCPTDIPPFLSFFSSTADSASLLKRLTYDRFSIMMLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKISSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSDPALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWLDDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Homology
BLAST of Lcy03g013460 vs. ExPASy Swiss-Prot
Match: O58403 (Aspartate racemase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=PH0670 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.6e-14
Identity = 67/251 (26.69%), Postives = 113/251 (45.02%), Query Frame = 0

Query: 117 TVGVIGGVSVISTL-LFLEKLVWWSLKDGQESIPFVVCSDP--------ALGKGIFPLTP 176
           T+G++GG+  ++T  LF   ++    K  QE    ++ ++P         LGKG  P  P
Sbjct: 3   TIGILGGMGPLATAELFRRIVIKTPAKRDQEHPKVIIFNNPQIPDRTAYILGKGEDP-RP 62

Query: 177 SSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWLDDTTESCKLPFL 236
              ++A                  KR  LE+ GA  +I PC+ +H +++D  ++ K+P +
Sbjct: 63  QLIWTA------------------KR--LEECGADFIIMPCNTAHAFVEDIRKAIKIPII 122

Query: 237 HVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMK 296
            + +  A ++KE   K        + GLLAT   IV+G Y +     G +++ P E   K
Sbjct: 123 SMIEETAKKVKELGFK--------KAGLLATTGTIVSGVYEKEFSKYGVEIMTPTEDEQK 182

Query: 297 HIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCID 356
            ++    E     + +  R LL     +L  R    +I    E+  +L  DD +    ID
Sbjct: 183 DVMRGIYEGVKAGNLKLGRELLLKTAKILEERGAECIIAGCTEVSVVLKQDD-LKVPLID 223

Query: 357 PMDALARAAIK 359
           PMD +A  A+K
Sbjct: 243 PMDVIAEVAVK 223

BLAST of Lcy03g013460 vs. ExPASy TrEMBL
Match: A0A6J1L4S4 (uncharacterized protein LOC111499133 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499133 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.4e-164
Identity = 296/335 (88.36%), Postives = 313/335 (93.43%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGMAMPFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKKI
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           SS+GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  SSAGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT  +TF+A+SSQH  +DAPIIE LKQK  FLEQSGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

BLAST of Lcy03g013460 vs. ExPASy TrEMBL
Match: A0A6J1EU92 (uncharacterized protein LOC111437803 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437803 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.0e-164
Identity = 295/335 (88.06%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGMAMPFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKKI
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT  +TF A+SSQH  +DAPIIE LKQK  FLEQSGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 SDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

BLAST of Lcy03g013460 vs. ExPASy TrEMBL
Match: A0A5A7TXR8 (Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00390 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.4e-157
Identity = 285/332 (85.84%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 35  MMLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKK 94
           MML GGMAM FY   CPAP+RRNAN+ IT FRRR+N YSSVQISSV+QTD NDNLP SKK
Sbjct: 1   MMLHGGMAMHFYAPICPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKK 60

Query: 95  ISSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCS 154
           ISS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS
Sbjct: 61  ISSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCS 120

Query: 155 DPALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKW 214
           +PALGKGI P+T   TFS  SSQ+GHSDAPIIENL +KRAFLE SGARCLITPCHL+H+W
Sbjct: 121 EPALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRW 180

Query: 215 LDDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQ 274
           L DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQ
Sbjct: 181 LSDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQ 240

Query: 275 GFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 334
           GFDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL
Sbjct: 241 GFDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 300

Query: 335 LPPDDPILKKCIDPMDALARAAIKWSRSTGNL 367
           LPPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 301 LPPDDPILKKCIDPMDALARAAIKWSRSTENL 331

BLAST of Lcy03g013460 vs. ExPASy TrEMBL
Match: A0A1S3CMU2 (uncharacterized protein LOC103502741 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502741 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.4e-157
Identity = 285/332 (85.84%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 35  MMLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKK 94
           MML GGMAM FY   CPAP+RRNAN+ IT FRRR+N YSSVQISSV+QTD NDNLP SKK
Sbjct: 1   MMLHGGMAMHFYAPICPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKK 60

Query: 95  ISSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCS 154
           ISS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS
Sbjct: 61  ISSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCS 120

Query: 155 DPALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKW 214
           +PALGKGI P+T   TFS  SSQ+GHSDAPIIENL +KRAFLE SGARCLITPCHL+H+W
Sbjct: 121 EPALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRW 180

Query: 215 LDDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQ 274
           L DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQ
Sbjct: 181 LSDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQ 240

Query: 275 GFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 334
           GFDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL
Sbjct: 241 GFDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 300

Query: 335 LPPDDPILKKCIDPMDALARAAIKWSRSTGNL 367
           LPPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 301 LPPDDPILKKCIDPMDALARAAIKWSRSTENL 331

BLAST of Lcy03g013460 vs. ExPASy TrEMBL
Match: A0A5D3E266 (Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G00430 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 284/332 (85.54%), Postives = 300/332 (90.36%), Query Frame = 0

Query: 35  MMLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKK 94
           MML GGMAM FY    PAP+RRNAN+ IT FRRR+N YSSVQISSV+QTD NDNLP SKK
Sbjct: 1   MMLHGGMAMHFYAPIFPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKK 60

Query: 95  ISSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCS 154
           ISS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS
Sbjct: 61  ISSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCS 120

Query: 155 DPALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKW 214
           +PALGKGI P+T   TFS  SSQ+GHSDAPIIENL +KRAFLE SGARCLITPCHL+H+W
Sbjct: 121 EPALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRW 180

Query: 215 LDDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQ 274
           L DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQ
Sbjct: 181 LSDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQ 240

Query: 275 GFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 334
           GFDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL
Sbjct: 241 GFDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 300

Query: 335 LPPDDPILKKCIDPMDALARAAIKWSRSTGNL 367
           LPPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 301 LPPDDPILKKCIDPMDALARAAIKWSRSTENL 331

BLAST of Lcy03g013460 vs. NCBI nr
Match: XP_038878952.1 (uncharacterized protein LOC120071036 [Benincasa hispida])

HSP 1 Score: 595.9 bits (1535), Expect = 2.4e-166
Identity = 297/335 (88.66%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGM   FY L CPAPIRRNAN+ IT FRRR+N YSSVQISSVIQTD NDNLPGSKKI
Sbjct: 1   MLDGGMTTSFYALTCPAPIRRNANEGITRFRRRINPYSSVQISSVIQTDANDNLPGSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           SSSGKSLSK+RTPKPLLVQP TVGVIGGVSV STLLFLEKLVWWS+KDGQESIPFVVCSD
Sbjct: 61  SSSGKSLSKTRTPKPLLVQPKTVGVIGGVSVFSTLLFLEKLVWWSMKDGQESIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT S TFS +SSQ+GHSD PIIENLK+KRAFLEQSGARCLITPCHL+H+WL
Sbjct: 121 PALGKGIPPLTSSCTFSTNSSQYGHSDDPIIENLKRKRAFLEQSGARCLITPCHLTHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT+ESCKLPFLHVGDCVA ELKEAKLKPLE GSSVRIGLLATD A V GFYHERLQNQG
Sbjct: 181 GDTSESCKLPFLHVGDCVARELKEAKLKPLEGGSSVRIGLLATDTATVAGFYHERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           FD++LPDEATM+HIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL
Sbjct: 241 FDILLPDEATMEHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRST NLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTENLHEKA 335

BLAST of Lcy03g013460 vs. NCBI nr
Match: XP_023006388.1 (uncharacterized protein LOC111499133 isoform X1 [Cucurbita maxima])

HSP 1 Score: 589.0 bits (1517), Expect = 2.9e-164
Identity = 296/335 (88.36%), Postives = 313/335 (93.43%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGMAMPFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKKI
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           SS+GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  SSAGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT  +TF+A+SSQH  +DAPIIE LKQK  FLEQSGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

BLAST of Lcy03g013460 vs. NCBI nr
Match: XP_023530993.1 (uncharacterized protein LOC111793383 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.2 bits (1515), Expect = 5.0e-164
Identity = 296/336 (88.10%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 35  MMLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKK 94
           MMLDGGMAMPFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKK
Sbjct: 1   MMLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKK 60

Query: 95  ISSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCS 154
           IS+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCS
Sbjct: 61  ISTPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCS 120

Query: 155 DPALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKW 214
           DPALGKGI PLT  +TF A+SSQH  +DAPIIE LKQK  FLEQSGARCLITPCHLSH+W
Sbjct: 121 DPALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRW 180

Query: 215 LDDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQ 274
           L DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQ
Sbjct: 181 LGDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQ 240

Query: 275 GFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNL 334
           GF+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNL
Sbjct: 241 GFEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNL 300

Query: 335 LPPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           LPPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 LPPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 334

BLAST of Lcy03g013460 vs. NCBI nr
Match: XP_022931647.1 (uncharacterized protein LOC111437803 isoform X1 [Cucurbita moschata])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 295/335 (88.06%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGMAMPFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKKI
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT  +TF A+SSQH  +DAPIIE LKQK  FLEQSGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 SDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

BLAST of Lcy03g013460 vs. NCBI nr
Match: KAG7021803.1 (hypothetical protein SDJN02_15531, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 579.7 bits (1493), Expect = 1.8e-161
Identity = 291/335 (86.87%), Postives = 309/335 (92.24%), Query Frame = 0

Query: 36  MLDGGMAMPFYGLNCPAPIRRNANKRITHFRRRLNQYSSVQISSVIQTDENDNLPGSKKI 95
           MLDGGMA+PFY LNCPAP+RRNAN+ IT FRRRLN YSS+QI SV+QTDENDNLP SKKI
Sbjct: 1   MLDGGMAVPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 96  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 155
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 156 PALGKGIFPLTPSSTFSASSSQHGHSDAPIIENLKQKRAFLEQSGARCLITPCHLSHKWL 215
           PALGKGI PLT  +TF+ +SSQH   DAPIIE LKQK  FLEQSGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTENSSQHAQIDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 216 DDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 275
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 276 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 335
           F+VVLPD+AT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDE LNLL
Sbjct: 241 FEVVLPDDATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDEFLNLL 300

Query: 336 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 371
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

BLAST of Lcy03g013460 vs. TAIR 10
Match: AT1G15410.1 (aspartate-glutamate racemase family )

HSP 1 Score: 302.0 bits (772), Expect = 6.7e-82
Identity = 152/303 (50.17%), Postives = 217/303 (71.62%), Query Frame = 0

Query: 66  RRRLNQYSSVQISSV-IQTDENDNLPGSKK---ISSSGKSLSKSRTPKPLLVQPNTVGVI 125
           R RL++  ++  SSV +  DE+++LP  KK   +S   ++   S     LL Q NTVG+I
Sbjct: 25  RVRLSRVLAMPPSSVLLHMDESNDLPKPKKGFCLSEDSRNSLNSLPADSLLRQANTVGII 84

Query: 126 GGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSDPALGKGIFPLTPSSTFSASSSQHGHS 185
           GGVS  STL F++KLV WS  DG+ S+PFV+CSDPAL K +     +S  S    +    
Sbjct: 85  GGVSTDSTLKFVKKLVDWSSNDGKSSLPFVLCSDPALNKELLLYEENSYPSLYHREESTP 144

Query: 186 DAP--IIENLKQKRAFLEQSGARCLITPCHLSHKWLDDTTESCKLPFLHVGDCVAMELKE 245
             P  I+ENL+ KR +LE+ GA+ ++ PCH++H W ++  E   +P LH+G+C+A EL+E
Sbjct: 145 VDPKLIVENLRNKRRYLERCGAKLILMPCHIAHIWYEEVCEGSLVPLLHMGECIAKELQE 204

Query: 246 AKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMKHIVVPAVEAFNK 305
           AK+KPLEAG+ +R+G++AT   +  GFY E+LQ+ GF+ VLPD+ATM+H V+P++EA  +
Sbjct: 205 AKMKPLEAGNPLRVGVMATSATLSAGFYQEKLQSNGFEAVLPDKATMEHTVIPSIEAMKR 264

Query: 306 RDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCIDPMDALARAAIKW 363
           +D EGARNLLRIA+ VLL++AVN+V+L SDE+ +LLP DDP+LKKC+DPMDALAR+AIKW
Sbjct: 265 KDMEGARNLLRIALQVLLVQAVNVVMLGSDEMRDLLPGDDPLLKKCVDPMDALARSAIKW 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O584032.6e-1426.69Aspartate racemase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JC... [more]
Match NameE-valueIdentityDescription
A0A6J1L4S41.4e-16488.36uncharacterized protein LOC111499133 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EU927.0e-16488.06uncharacterized protein LOC111437803 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7TXR86.4e-15785.84Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3CMU26.4e-15785.84uncharacterized protein LOC103502741 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3E2661.2e-15585.54Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
XP_038878952.12.4e-16688.66uncharacterized protein LOC120071036 [Benincasa hispida][more]
XP_023006388.12.9e-16488.36uncharacterized protein LOC111499133 isoform X1 [Cucurbita maxima][more]
XP_023530993.15.0e-16488.10uncharacterized protein LOC111793383 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022931647.11.5e-16388.06uncharacterized protein LOC111437803 isoform X1 [Cucurbita moschata][more]
KAG7021803.11.8e-16186.87hypothetical protein SDJN02_15531, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT1G15410.16.7e-8250.17aspartate-glutamate racemase family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015942Asp/Glu/hydantoin racemasePFAMPF01177Asp_Glu_racecoord: 121..356
e-value: 2.3E-24
score: 86.4
NoneNo IPR availableGENE3D3.40.50.1860coord: 117..354
e-value: 1.0E-41
score: 145.1
NoneNo IPR availableGENE3D3.40.50.1860coord: 231..348
e-value: 1.0E-41
score: 145.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..108
NoneNo IPR availablePANTHERPTHR21198GLUTAMATE RACEMASEcoord: 36..366
NoneNo IPR availablePANTHERPTHR21198:SF9ASPARTATE RACEMASEcoord: 36..366
IPR001920Asp/Glu racemaseSUPERFAMILY53681Aspartate/glutamate racemasecoord: 117..239
IPR001920Asp/Glu racemaseSUPERFAMILY53681Aspartate/glutamate racemasecoord: 252..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g013460.1Lcy03g013460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0036361 racemase activity, acting on amino acids and derivatives
molecular_function GO:0016855 racemase and epimerase activity, acting on amino acids and derivatives