Lcy03g010380 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g010380
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr03: 44165736 .. 44168506 (-)
RNA-Seq ExpressionLcy03g010380
SyntenyLcy03g010380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTCTCTCTCTCTCTCCCCTTGATCCAAATCTTCACCCACTGTTCTTGTTTCTCCCTCTTCTGTAGCCTCCACTGCTCCTACTTCAGCTCCGGTACGTCTTTCTTTACTTCAATTCCTGATCAATTTCAAATTACGGATCTATATTTCTGTTTCTGCATCTTTCGTATTTGATTCATTATGTATCTGTGGTGGATTGTTGTTTGATGCATGAAATTCTCTGAACTCAGTGACCGATCTGATATCTGGCGAGATTCTTGTTAATTTCGATGATTTTCGAGTGTTTCCTTGTGGTTTTGGTCAAATCGGACGAGGGATTGAATGCCTCGTTTGAGTGATGATTCTGTTCGAGGAGATGGTGCCTCTACTGAGGGGAATTTCCTCTCAGCTACAGGAGATTGTCTCCCTTGCTTGTGTGAGACGAGGAATGTGGGAATCATACGGATGCTGCATCGAGATGTTTGTCAGAAGAAATGGGGACAGTGGATGTACTCATATTAATTGCTAGCTATCGAGTTGAAATTATGGAGTGTTTTGCCTATTTGAGTTCAGTTACGTTTAAGTAAGCCACATGAACGTGTTTGGGATTTGTCATTTTTTGTAGTTAAATTACATTTTTAGTCTATATGTTTTCATTTCTGTCTCTATTTCTCTTTTATTTGTCAAAGCTTACTGAATAGTCTTAAGACTCTTAACAATAGTGAAGAATCACATATCTAATGTAGTAAACCAAAATAATACTTTAAAATTTGACCCAAATTCGTATTTTTCAACTAGTATGACCTTGTAGACACTCTACTAAACTGGGGATTTTTGGTAAACTTTAATCAATCAAACGTCTTGAAAGTACATGGACCAAATTGATATATAGGTGAAAATATAGGGAGTAAAATGTAAACTCTGCTTTTCTCTTGAATAGTGGCGTATCCAATCAATGAGTATATCCCTTGATTCCTTTCCAAGACTAATTGGATGTCTCTTGCATGGCTTGGCTAAGCTTATACAACACTAATCCTTTATTATGGTCTGGGTTTTCTTTCACTATTTTTGTTTAAATCTTTAGGGAAACCATCACCTGAAATGTCTCCATTTTAAGGACATTTTCTGAATTTAACTAAGTTGCTTGATTCTTTTCCAAATTGTATTCCACGAACAGTCAAATTATTCTGTGATTTTGAAAAGCTATTCCTGGGATGAATAGATGTTATATATTAGTTTTTCACATACATTAGTCTATTGTGAATAAATGTTTGATTTTGTCTTCATAATTGATTTTCTTAAGCAATTAGCATTTCATTGCAGAATCCTCTACATTTAGTAGAAAACCTGGGCTTTTCTTTCAGCTGTTGCATTTGAGAGGAGGATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACGGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGATGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCTTTTCAATTCCTTTTGGTCATTCCATGAACATGTTACTGTTCCGACCTACACTAGCATCACTCCGGAAGATCGATCTAGTCCACGTTCGGCACTGGAAATATTAACTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGAGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAAATGAGAGAGAGATTATTCAACGAAATCTGAGAAGGAGGTTTGGATTCCCAGAGTTGTTTACTCCTCGAAATGTAGTCACTCGTTTCAGCAGCGGGGTTCGTAGAAATGGTGCATCTACAAATCGCGAGCACGATAATGAGACGAGTTACCCTGAATGGCCTTTCAATTTCTAAGTCTATAGCTCTTCTCTACTTGAACATGACATCTGAAACCTGTGTTCCACAACAAATGGGGCAAATAAGAAATAAGAATCAGCTCTAGAGTTGTGATATCTAGAGCTGTTTTTTCCCCCCTTTTTGTGTATAATGATATGAAAAAGTTCACATCATCTGCTGCTTTGACTTGCACAGGGAAGAAGGGGTTTCAGAGCTGAAATCAATTGTAATTATAGTTAATAGTTATGTTCCAAGTTCCATGGATCAATTGAGAAGCCACTTGCTTTTTCTGATTTGTGAGCTGCTTTATGATAGTTATAAGTGATTGAGAAGGTGATATCTTATATGGGTAAGATTTGAAAAGGGAGCAAGAGATTTTATTCTGTGTTTGGCTGTGCATTTTGGCATCTGTGATAATACATTCATTCACTTATTGCATCTAAGATTCTCACAGGTGGGCTGAAATGACAATTGC

mRNA sequence

TCTCTCTCTCTCTCTCTCCCCTTGATCCAAATCTTCACCCACTGTTCTTGTTTCTCCCTCTTCTGTAGCCTCCACTGCTCCTACTTCAGCTCCGTGACCGATCTGATATCTGGCGAGATTCTTGTTAATTTCGATGATTTTCGAGTGTTTCCTTGTGGTTTTGGTCAAATCGGACGAGGGATTGAATGCCTCGTTTGAGTGATGATTCTGTTCGAGGAGATGGTGCCTCTACTGAGGGGAATTTCCTCTCAGCTACAGGAGATTGTCTCCCTTGCTTGTGTGAGACGAGGAATGTGGGAATCATACGGATGCTGCATCGAGATGTTTGTCAGAAGAAATGGGGACAGTGGATGTACTCATATTAATTGCTAGCTATCGAGTTGAAATTATGGAGTGTTTTGCCTATTTGAGTTCAGTTACGTTTAAAATCCTCTACATTTAGTAGAAAACCTGGGCTTTTCTTTCAGCTGTTGCATTTGAGAGGAGGATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACGGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGATGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCTTTTCAATTCCTTTTGGTCATTCCATGAACATGTTACTGTTCCGACCTACACTAGCATCACTCCGGAAGATCGATCTAGTCCACGTTCGGCACTGGAAATATTAACTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGAGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAAATGAGAGAGAGATTATTCAACGAAATCTGAGAAGGAGGTTTGGATTCCCAGAGTTGTTTACTCCTCGAAATGTAGTCACTCGTTTCAGCAGCGGGGTTCGTAGAAATGGTGCATCTACAAATCGCGAGCACGATAATGAGACGAGTTACCCTGAATGGCCTTTCAATTTCTAAGTCTATAGCTCTTCTCTACTTGAACATGACATCTGAAACCTGTGTTCCACAACAAATGGGGCAAATAAGAAATAAGAATCAGCTCTAGAGTTGTGATATCTAGAGCTGTTTTTTCCCCCCTTTTTGTGTATAATGATATGAAAAAGTTCACATCATCTGCTGCTTTGACTTGCACAGGGAAGAAGGGGTTTCAGAGCTGAAATCAATTGTAATTATAGTTAATAGTTATGTTCCAAGTTCCATGGATCAATTGAGAAGCCACTTGCTTTTTCTGATTTGTGAGCTGCTTTATGATAGTTATAAGTGATTGAGAAGGTGATATCTTATATGGGTAAGATTTGAAAAGGGAGCAAGAGATTTTATTCTGTGTTTGGCTGTGCATTTTGGCATCTGTGATAATACATTCATTCACTTATTGCATCTAAGATTCTCACAGGTGGGCTGAAATGACAATTGC

Coding sequence (CDS)

ATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACGGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGATGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCTTTTCAATTCCTTTTGGTCATTCCATGAACATGTTACTGTTCCGACCTACACTAGCATCACTCCGGAAGATCGATCTAGTCCACGTTCGGCACTGGAAATATTAACTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGAGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAAATGAGAGAGAGATTATTCAACGAAATCTGAGAAGGAGGTTTGGATTCCCAGAGTTGTTTACTCCTCGAAATGTAGTCACTCGTTTCAGCAGCGGGGTTCGTAGAAATGGTGCATCTACAAATCGCGAGCACGATAATGAGACGAGTTACCCTGAATGGCCTTTCAATTTCTAA

Protein sequence

MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLFNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTNREHDNETSYPEWPFNF
Homology
BLAST of Lcy03g010380 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-43
Identity = 127/356 (35.67%), Postives = 169/356 (47.47%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVD------LF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++        +F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 NSFWSFH----EHVTVPTYTSITPEDRSSPRS----------ALEILTAQLSL-NEHRDP 186
                F     ++ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 246
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 245

Query: 247 LVQHNTCPVCRLELPQQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNV 306
           LVQHN+CPVCR ELP + S      ++ S+ +   N RE    N RRR  F  L+  R+ 
Sbjct: 246 LVQHNSCPVCRKELPSRGS----SSSTQSSQNRSTNGRE----NSRRRNIFSNLWPFRS- 305

Query: 307 VTRFSSGVRRNGASTN---------------------REHDNETS---YPEWPFNF 317
               SS   +N   TN                      +H  + S   Y  WPF++
Sbjct: 306 ---SSSSSTQNRRDTNNTATAEEGHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFDY 320

BLAST of Lcy03g010380 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 9.6e-42
Identity = 124/352 (35.23%), Postives = 166/352 (47.16%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHEHVTVPTYTSITPE---DRSSP--------------RSALEILTAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 242
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQDSGHDWGGN--------SSSNNSGVLNEREIIQRNLRRRFGFP 302
           LVQHN+CPVCR ELP   SG     N         SS++S   N RE       RR  F 
Sbjct: 242 LVQHNSCPVCRQELPSA-SGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 ELFTPRNVVTRFSSGVRRNGASTNREHDNETSYPE------------WPFNF 317
             +  R+  +  SS   R G   +   D   +Y +            WPF++
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHNYHQQQHQQSYMGYSGWPFDY 328

BLAST of Lcy03g010380 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.2e-30
Identity = 94/261 (36.02%), Postives = 134/261 (51.34%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDF--NSGTEDDL 60
           M+SG   ++WCY C+   W++ +   CP C+GGF+E + +  D T  + F   + T+   
Sbjct: 1   MASG---SYWCYSCSRFVWVS-DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRS 60

Query: 61  STQVPPIFEA----MFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPG 120
            T+ PP   +      ++    SP P           TR R     +   V R S++ P 
Sbjct: 61  PTRFPPPSSSSSTPSASMHADNSPTPTI--------VTRTRSNRSPNPVIVLRGSAAAPS 120

Query: 121 QNVDL----FNSFWSFHEHVT----VPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPV 180
            +V       ++F  +++  T     P   S+T     S    L    +Q+ LN +R+  
Sbjct: 121 SDVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLR 180

Query: 181 PA---PASHSSIEAMPTIKINQMHLGTD--SHCPVCKEKFELGSEAKEMPCNHIYHTDCI 240
                PAS S+IEA+P I+I+  HL +D  SHC VCKE F L S A+EMPCNHIYH DCI
Sbjct: 181 SCEHPPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCI 240

Query: 241 LPWLVQHNTCPVCRLELPQQD 243
           LPWL   N+CPVCR ELP +D
Sbjct: 241 LPWLAIRNSCPVCRHELPAED 249

BLAST of Lcy03g010380 vs. ExPASy Swiss-Prot
Match: Q7T0Q3 (E3 ubiquitin-protein ligase RNF126-A OS=Xenopus laevis OX=8355 GN=rnf126-a PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.6e-28
Identity = 78/272 (28.68%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 9   HWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEA 68
           ++C+ C         +  CP C+ GF+EEL     ET +N  N+ + +  + Q  P FE 
Sbjct: 11  YFCHSCTAEITPRLPEYTCPRCDSGFIEEL----PETSRNSENNSSNNSRTDQNRPSFEN 70

Query: 69  M---------------FALMGRRSPYPRFGLLEAVDAF----------TRERMAGRNSNF 128
           +               F +      +P FG    V+            +R+R   R    
Sbjct: 71  LESAQFTLPSGYGQVTFGIFNEGLDFPIFGTSGPVEETRDGESRREHQSRQRYGARQPRA 130

Query: 129 DVRRRSSSIPGQNV-DLFNSFWSFHEHVTVPTYTS-----------ITPEDRSSPRSALE 188
            +  R ++   + V  L          +  PT  S             P D +   + L+
Sbjct: 131 RLSTRRAAGRNEGVPTLEGIIQQLVNGIIAPTAMSNLGVGPWGVLHSNPMDYAWGANGLD 190

Query: 189 ILTAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMP 244
            +  QL LN+  +  P PA +  I+A+PTI+I + H+G+   CPVCKE + +G   +++P
Sbjct: 191 TIITQL-LNQFENTGPPPADNEKIQALPTIQITEEHVGSGLECPVCKEDYTVGECVRQLP 250

BLAST of Lcy03g010380 vs. ExPASy Swiss-Prot
Match: Q6DIP3 (E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 78/272 (28.68%), Postives = 122/272 (44.85%), Query Frame = 0

Query: 9   HWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEA 68
           ++C+ C         +  CP C+ GF+EEL +  +    +  NSGT+     Q  P FE 
Sbjct: 11  YFCHSCTAEIIPRLPEYTCPRCDSGFIEELPETRNSENNSSNNSGTD-----QNRPSFEN 70

Query: 69  M---------------FALMGRRSPYPRFGLLEAVDA----------FTRERMAGRNSNF 128
           +               F +      +P FG    V+            +R+R   R    
Sbjct: 71  LESAQFTLPSGYGQVTFGIFNEGLDFPIFGTSGPVEEPRDGESRREHQSRQRYGARQPRA 130

Query: 129 DVRRRSSSIPGQNV-DLFNSFWSFHEHVTVPTYTS-----------ITPEDRSSPRSALE 188
            +  R ++   + V  L          +  PT  S             P D +   + L+
Sbjct: 131 RLSTRRAAGRNEGVPTLEGIIQQLVNGIIAPTAMSNLGVGPWGVLHSNPMDYAWGANGLD 190

Query: 189 ILTAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMP 244
            +  QL LN+  +  P PA    I+A+PTI+I + H+G+   CPVCKE + +G   +++P
Sbjct: 191 TIITQL-LNQFENTGPPPADTEKIQALPTIQITEEHVGSGLECPVCKEDYTVGESVRQLP 250

BLAST of Lcy03g010380 vs. ExPASy TrEMBL
Match: A0A1S3BQN3 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.0e-147
Identity = 244/311 (78.46%), Postives = 270/311 (86.82%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPT 180
           NSFWSFH+H++  T+ ++TPE RSS  + LE L AQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTN 300
           Q+SGH WGGN  SN+   LNERE+ QRNLRRR  FP+ F PR +VTRF SG+  NG S++
Sbjct: 241 QESGHSWGGNGGSNSED-LNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSD 300

Query: 301 REHDNETSYPE 312
           R+HDNE  YPE
Sbjct: 301 RDHDNEMGYPE 310

BLAST of Lcy03g010380 vs. ExPASy TrEMBL
Match: A0A5A7UIL6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00680 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.0e-147
Identity = 244/311 (78.46%), Postives = 270/311 (86.82%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPT 180
           NSFWSFH+H++  T+ ++TPE RSS  + LE L AQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTN 300
           Q+SGH WGGN  SN+   LNERE+ QRNLRRR  FP+ F PR +VTRF SG+  NG S++
Sbjct: 241 QESGHSWGGNGGSNSED-LNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSD 300

Query: 301 REHDNETSYPE 312
           R+HDNE  YPE
Sbjct: 301 RDHDNEMGYPE 310

BLAST of Lcy03g010380 vs. ExPASy TrEMBL
Match: A0A6J1D235 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 249/319 (78.06%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDL 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMP 180
           FNSFWSFHEHV  P++ S+ PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDW--GGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGA 300
           QQD+ H W  G +SSS+N  V N REI Q  L RR    + +T  NV+ RFSSG+RRNG 
Sbjct: 241 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 300

Query: 301 STNREHDNETSYPEWPFNF 317
           STN  HDNE SYPEWPF++
Sbjct: 301 STNHYHDNEMSYPEWPFHY 319

BLAST of Lcy03g010380 vs. ExPASy TrEMBL
Match: A0A6J1D364 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 249/319 (78.06%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDL 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMP 180
           FNSFWSFHEHV  P++ S+ PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQDSGHDW--GGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGA 300
           QQD+ H W  G +SSS+N  V N REI Q  L RR    + +T  NV+ RFSSG+RRNG 
Sbjct: 297 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 356

Query: 301 STNREHDNETSYPEWPFNF 317
           STN  HDNE SYPEWPF++
Sbjct: 357 STNHYHDNEMSYPEWPFHY 375

BLAST of Lcy03g010380 vs. ExPASy TrEMBL
Match: A0A0A0LZS8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.9e-146
Identity = 245/311 (78.78%), Postives = 268/311 (86.17%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWC+QC+HSFWL+GED VCP+C GGFVEELND HDETVQNDFN G E+DLST
Sbjct: 59  MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 118

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 119 QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 178

Query: 121 NSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPT 180
           NSFWSFH+H++  T+ ++TPE RSS  + LE L AQLSLNE R+PVP PASHS IEAMPT
Sbjct: 179 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 238

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFEL SEAK +PCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 239 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 298

Query: 241 QDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTN 300
           Q+SGH WGG S  NNS  LNEREI QRNLRRR   P+ F PR +VTRFSSG+  NG S++
Sbjct: 299 QESGHSWGG-SGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSD 358

Query: 301 REHDNETSYPE 312
           R+HDNE  YPE
Sbjct: 359 RDHDNEMGYPE 368

BLAST of Lcy03g010380 vs. NCBI nr
Match: XP_008451039.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451041.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0055692.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09941.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 244/311 (78.46%), Postives = 270/311 (86.82%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPT 180
           NSFWSFH+H++  T+ ++TPE RSS  + LE L AQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTN 300
           Q+SGH WGGN  SN+   LNERE+ QRNLRRR  FP+ F PR +VTRF SG+  NG S++
Sbjct: 241 QESGHSWGGNGGSNSED-LNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSD 300

Query: 301 REHDNETSYPE 312
           R+HDNE  YPE
Sbjct: 301 RDHDNEMGYPE 310

BLAST of Lcy03g010380 vs. NCBI nr
Match: XP_022147727.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 249/319 (78.06%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDL 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMP 180
           FNSFWSFHEHV  P++ S+ PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQDSGHDW--GGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGA 300
           QQD+ H W  G +SSS+N  V N REI Q  L RR    + +T  NV+ RFSSG+RRNG 
Sbjct: 297 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 356

Query: 301 STNREHDNETSYPEWPFNF 317
           STN  HDNE SYPEWPF++
Sbjct: 357 STNHYHDNEMSYPEWPFHY 375

BLAST of Lcy03g010380 vs. NCBI nr
Match: XP_022147728.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 249/319 (78.06%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDL 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMP 180
           FNSFWSFHEHV  P++ S+ PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDW--GGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGA 300
           QQD+ H W  G +SSS+N  V N REI Q  L RR    + +T  NV+ RFSSG+RRNG 
Sbjct: 241 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 300

Query: 301 STNREHDNETSYPEWPFNF 317
           STN  HDNE SYPEWPF++
Sbjct: 301 STNHYHDNEMSYPEWPFHY 319

BLAST of Lcy03g010380 vs. NCBI nr
Match: XP_004144098.1 (E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_031736051.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >KAE8653480.1 hypothetical protein Csa_007077 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 4.0e-146
Identity = 245/311 (78.78%), Postives = 268/311 (86.17%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWC+QC+HSFWL+GED VCP+C GGFVEELND HDETVQNDFN G E+DLST
Sbjct: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMPT 180
           NSFWSFH+H++  T+ ++TPE RSS  + LE L AQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFEL SEAK +PCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNGASTN 300
           Q+SGH WGG S  NNS  LNEREI QRNLRRR   P+ F PR +VTRFSSG+  NG S++
Sbjct: 241 QESGHSWGG-SGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSD 300

Query: 301 REHDNETSYPE 312
           R+HDNE  YPE
Sbjct: 301 RDHDNEMGYPE 310

BLAST of Lcy03g010380 vs. NCBI nr
Match: KAG6588139.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 527.7 bits (1358), Expect = 6.8e-146
Identity = 256/319 (80.25%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSSGGEDTHWCYQCN SFWLNGED  CPYCNGGF+EELNDVHD+ VQ+DF+S  EDDLS+
Sbjct: 1   MSSGGEDTHWCYQCNRSFWLNGEDIACPYCNGGFIEELNDVHDDAVQDDFHSAIEDDLSS 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLL-EAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDL 120
           QVPPIFEAMFALMGRR+PYPRF LL EAVDA TRERMAGRN N DVRRRS+SIPGQN+D+
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFRLLDEAVDALTRERMAGRNPNIDVRRRSNSIPGQNLDI 120

Query: 121 FNSFWSFHEHVTVPTYTSITPEDRSSPRSALEILTAQLSLNEHRDPVPAPASHSSIEAMP 180
           FNSFWSFHEH   P++ S+ PE RSS   ALE L A LS NE RDPVP PASHSSIEAMP
Sbjct: 121 FNSFWSFHEHAPGPSFASVAPEGRSSQHVALEALAA-LSFNEQRDPVPTPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTD+HCPVCKEKFELGSEAKEMPCNHIYH DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDTHCPVCKEKFELGSEAKEMPCNHIYHADCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNVVTRFSSGVRRNG-AS 300
           QQDS H WGGNSSS NS V NEREII   LRRRF FP+ FTP++  TRFSS + RNG AS
Sbjct: 241 QQDSRHGWGGNSSS-NSEVSNEREII---LRRRFAFPDFFTPQSAGTRFSSRIGRNGRAS 300

Query: 301 TNREHDNETSYPEW-PFNF 317
           TNRE DNE SYPEW PFN+
Sbjct: 301 TNREQDNEISYPEWPPFNY 314

BLAST of Lcy03g010380 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 127/356 (35.67%), Postives = 169/356 (47.47%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVD------LF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++        +F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 NSFWSFH----EHVTVPTYTSITPEDRSSPRS----------ALEILTAQLSL-NEHRDP 186
                F     ++ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 246
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 245

Query: 247 LVQHNTCPVCRLELPQQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNV 306
           LVQHN+CPVCR ELP + S      ++ S+ +   N RE    N RRR  F  L+  R+ 
Sbjct: 246 LVQHNSCPVCRKELPSRGS----SSSTQSSQNRSTNGRE----NSRRRNIFSNLWPFRS- 305

Query: 307 VTRFSSGVRRNGASTN---------------------REHDNETS---YPEWPFNF 317
               SS   +N   TN                      +H  + S   Y  WPF++
Sbjct: 306 ---SSSSSTQNRRDTNNTATAEEGHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFDY 320

BLAST of Lcy03g010380 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 127/356 (35.67%), Postives = 169/356 (47.47%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVD------LF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++        +F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 NSFWSFH----EHVTVPTYTSITPEDRSSPRS----------ALEILTAQLSL-NEHRDP 186
                F     ++ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 246
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 245

Query: 247 LVQHNTCPVCRLELPQQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNV 306
           LVQHN+CPVCR ELP + S      ++ S+ +   N RE    N RRR  F  L+  R+ 
Sbjct: 246 LVQHNSCPVCRKELPSRGS----SSSTQSSQNRSTNGRE----NSRRRNIFSNLWPFRS- 305

Query: 307 VTRFSSGVRRNGASTN---------------------REHDNETS---YPEWPFNF 317
               SS   +N   TN                      +H  + S   Y  WPF++
Sbjct: 306 ---SSSSSTQNRRDTNNTATAEEGHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFDY 320

BLAST of Lcy03g010380 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 127/356 (35.67%), Postives = 169/356 (47.47%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVD------LF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++        +F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 NSFWSFH----EHVTVPTYTSITPEDRSSPRS----------ALEILTAQLSL-NEHRDP 186
                F     ++ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 246
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 245

Query: 247 LVQHNTCPVCRLELPQQDSGHDWGGNSSSNNSGVLNEREIIQRNLRRRFGFPELFTPRNV 306
           LVQHN+CPVCR ELP + S      ++ S+ +   N RE    N RRR  F  L+  R+ 
Sbjct: 246 LVQHNSCPVCRKELPSRGS----SSSTQSSQNRSTNGRE----NSRRRNIFSNLWPFRS- 305

Query: 307 VTRFSSGVRRNGASTN---------------------REHDNETS---YPEWPFNF 317
               SS   +N   TN                      +H  + S   Y  WPF++
Sbjct: 306 ---SSSSSTQNRRDTNNTATAEEGHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFDY 320

BLAST of Lcy03g010380 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 172.2 bits (435), Expect = 6.8e-43
Identity = 124/352 (35.23%), Postives = 166/352 (47.16%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHEHVTVPTYTSITPE---DRSSP--------------RSALEILTAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 242
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQDSGHDWGGN--------SSSNNSGVLNEREIIQRNLRRRFGFP 302
           LVQHN+CPVCR ELP   SG     N         SS++S   N RE       RR  F 
Sbjct: 242 LVQHNSCPVCRQELPSA-SGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 ELFTPRNVVTRFSSGVRRNGASTNREHDNETSYPE------------WPFNF 317
             +  R+  +  SS   R G   +   D   +Y +            WPF++
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHNYHQQQHQQSYMGYSGWPFDY 328

BLAST of Lcy03g010380 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 172.2 bits (435), Expect = 6.8e-43
Identity = 124/352 (35.23%), Postives = 166/352 (47.16%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDLFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHEHVTVPTYTSITPE---DRSSP--------------RSALEILTAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 242
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQDSGHDWGGN--------SSSNNSGVLNEREIIQRNLRRRFGFP 302
           LVQHN+CPVCR ELP   SG     N         SS++S   N RE       RR  F 
Sbjct: 242 LVQHNSCPVCRQELPSA-SGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 ELFTPRNVVTRFSSGVRRNGASTNREHDNETSYPE------------WPFNF 317
             +  R+  +  SS   R G   +   D   +Y +            WPF++
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHNYHQQQHQQSYMGYSGWPFDY 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AK43.0e-4335.67E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221979.6e-4235.23Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
O222832.2e-3036.02Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q7T0Q34.6e-2828.68E3 ubiquitin-protein ligase RNF126-A OS=Xenopus laevis OX=8355 GN=rnf126-a PE=2 ... [more]
Q6DIP31.0e-2728.68E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S3BQN31.0e-14778.46RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 ... [more]
A0A5A7UIL61.0e-14778.46RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1D2351.3e-14778.06RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
A0A6J1D3641.3e-14778.06RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
A0A0A0LZS81.9e-14678.78RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE... [more]
Match NameE-valueIdentityDescription
XP_008451039.12.1e-14778.46PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1... [more]
XP_022147727.12.8e-14778.06probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia][more]
XP_022147728.12.8e-14778.06probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia][more]
XP_004144098.14.0e-14678.78E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-... [more]
KAG6588139.16.8e-14680.25E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT3G56580.22.1e-4435.67RING/U-box superfamily protein [more]
AT3G56580.12.1e-4435.67RING/U-box superfamily protein [more]
AT3G56580.32.1e-4435.67RING/U-box superfamily protein [more]
AT2G40830.26.8e-4335.23RING-H2 finger C1A [more]
AT2G40830.16.8e-4335.23RING-H2 finger C1A [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 194..234
e-value: 3.9E-7
score: 39.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 193..235
e-value: 3.0E-11
score: 43.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 194..235
score: 12.25323
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 158..241
e-value: 1.7E-20
score: 74.5
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 8..38
e-value: 9.2E-11
score: 41.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..316
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..310
NoneNo IPR availablePANTHERPTHR15710:SF86E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATEDcoord: 3..310
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 194..235
e-value: 3.62499E-23
score: 88.1436
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 174..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g010380.1Lcy03g010380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity