Lcy03g009530 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g009530
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	cyanogenic beta-glucosidase-like
Location	Chr03: 42636735 .. 42640454 (+)
RNA-Seq Expression	Lcy03g009530
Synteny	Lcy03g009530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAATCTGCACCAGGCAGATGTTCCAAATGGCTACTTAATAACTGTTCGCATGGAGATTCCGCTACCGAACCATATATTGTTGGTCACCACCTCATTCTTGCTCATGCTCATGCAGTCGATATCTATCGCACGAAGTATCAGGTTCTATATGATCTCCCTCTAACTACGACGTGCATATTTAAAAAATTCTAGTGTTTGGCAACCACTCAAAAAATAACTTATGAAAAAGTGAGATTGAGAAATGAACTATTTTGAGAAAAACACTCGAGAGCAAATTTTTCAAAAAGAGTTTTTAGTATATAATGACTTGTGTATAAAAACAGAAATGTAACCAAACATACGAGTGTTTAATTAAATTCTAAACATTTTTTTTTAATACAAAATGTTTAAATAAAATGCATTCTTGAAAAACATTTAGGGTGTGTTTGGTTTAACTTTTTTAATTTTGAAAAAAAAAATTCTAGTGTTTGGCAACCACTTGTTAAAAAAATTAGATTGAGAAATACTTAATGTAACCAAACATTTAGGGTATTTCGAACGGATCGTAATTCGTTCACATATTCTCAAACCATTGATTTAATTTACAGACGGTACAAAAGGGTGTGATTGGCATGACTGTGGTGGCACATTGGTTTGCCCCATATGATGATACCGAGGCAAATATAAAGGCTGCACGTCGAGCTACTGATTTCTTGTCTGGTTGGTAAGTGTAAATTCATATATCTCAAACTTTATGAATCAATAATGATAATGCTACATTAAAATTATTTTCAAAACCTTTCTATTCAATATATTGTTCTCTCTACTAATGTTATAGGTACTTCAATCCAATCGTTCATGGTGATTATCCAGAAAGTATGATAATTCGTGTAAAAGATAGATTGCCAAGATTCACAATGAATGAGACCATTTTGATCAAAGGCAGCTATGATTTTCTTGGACTCAACTACTATACAGCTAGATATGCCATAGATAATTCCAATGAACATGTTCCATATCCATCCTACCTTACCGATTCTGGGGCAAATACTTCGCGTATGTGTTTAAGTGTATGTTTGGAATGATTTTTTTTAAGTGTTTAAAAATATTTTTTTAAGCTTAAAAACAACTTTTTAATCACTTAGGTCCAGTTTGATAACTATTTGGTTTTTGGTTTTTTAAAATTAAGCTTGTTTTCTCTCAATTTCTTTAGAATGGTTTTCATCTTTGTTAAGGACATATTTGCAATCCTAGCCAAATTCCAAAAAAAAAAAAAGTTTTTGAAAACTACGACAATCATTTGGGGGCCATTTGATAACCATTTCGTTTTCTCTGTTTCTGTTTTTCATTTAAAAAAAACAGGGTTGTTTGATAACCATTTCTGTTTTTTAAAAACGTTTTTGAAATTATAAGCAAATTTCAAAAAAAAAAAAAAAAAAATAGTTTTTAAAAATAGTTTTCTGGTTTTCAAAAACTCCTTTTTTTTTCCAATGCTTTTTTTTTTCTCTTTTACCTATCTCTCCCCAACAAATTCATTTTTCTGTTTTTTTCTATTACAACCTTTCTTTTTCTCTCTCTCAAAAATTTTTTTTTACTCTATTACTTATCTATTTCTAAAAATTTATCTATAACTTTGCTCCTCCTAAAAAACAAATTCTAACTTTTCTTTTGAAAAAAAAATCTCCCAAATTTTTTTCTAGCTAAAACTTTTCTTTATTTTTCTAATTTATCATTATTTCTCTAGCATATTTTTTACTCTTAACCCTACCATCTCTCTCAAATTTCTCTTTCTATTTAATGTTTCTATCTAAAATTTATCTCTTCTCTACTCTAACTTTTTTTCTTTTTTCTCTAACTAATATCTTTTATGTGTCTAACTAATATTCTCTCTCTAAACATTTTCTTTCACGTCTCTAAATTTTCTATCTTTAAATTTTCTCTCAAAAACATTTATATTCGTGTCTCTAAATTTTTGTTTCAATGTTCTACACATTTTTCCCTCTCTAAAACATCTTTTCATCTCTCTAATTTTTTAAAAATTGAAAACCAAAGTAGTTATCAAACAAGTTTGGTTTTCTGTTTTTTATAAATGGAGAACAAAAAACAAATATCAAACATGTATGGTTTTTGTTTTTAAAAAACAGAAAACGAAAAATAAAAAACTGAAATGGTTATCAAACGAGGCCTTGATTTTGGTTTTTTGGTTTTTGAATTAAGCTTATACACACCTACTAACATCTATAAGTTTCTTTGTTTTAGTATCAACCTTCTTATTTGTTTTCAAAAACCAAGTCCAATTTTGGAAACAAAAAAAATAGTTTTCAAAAACTTGCTTTTGTTTCTAAAATTTGGCTAAGAATTCAAATGGAGATTCTTAATAAGGATGAAAGCCATGATAGGGAATTTAAGAGAAAACAAACATAATTTTAAAAAACAAAAAACCAAATGGTTATCAAACGAGACCTACTTTTTTAGTTTTCAAAACTTGGGTTGGTTTTGGAACCCTGGGAAGAAGATAGATAACCACGGAAACAAATTTATAGGTTGAAGTAGTGTTTGTAGGCTCAATTTTAAAAAAACCAAAAATAAAAAACTTATCAACCCTGACCTTAGAAAGTCATTCCAAACCCACCTAAGATTAATCAAAATCCTATTTTTTTTTTTCAAACTCTTTAAGTTTATATATTTGAATTATTATTTTCTCGTGCAAATGAAATATTTGAACCATCTCTAACTTGACTTTGTACAATGTGCTTGGGTTTGTCCCTGCTCGTCATGGAGTTATGATTGGCCCACAGGTAATTTTATTTAAACTTTCTTCCACCATGAAAATTTGAAATATAATATGAATTCCTCGATTATAATATTTGATTATTCTTGTTCTAATTAATAAATTAATCTAACAATTTGTTAATTATTTATTAACAACGTTGTAGGTGAGTGGATCTTGGCTTTCAGTTTATCCACAAGGATTACAATATACCTTGATGGACCTGAAATATAATTATAAGGATCCAGTTATCTGCATCACAGAAAATGGTATTAAAAAAAATCTCACTTCTTTATAATCTTTAAAAAAAAAAAAAACAAAAACATCACATGATTAATTACCTTATTAATTAAATTCTACATTATGCAGGATATTTGGATCGCGATGGTCTTAATGTTTCTAAACTAATTCATGATACCAATAGAACGGATTACCATAGAGAACACCTCTATTATGTTCACAAAGCAATCGAGTAAGTAACTAACCGAAAGATTTTTTTCTGAATTGACTATAAAGAGTTGATTTGTAATTTATAGTGTTTTTGTGTCATAAACGAGTTCAACAACGATTGGGGGTGGGGGGATTCGAAACTCTGACCTCTTGGTCGAGGTTACATTCCAATTACTGTTGAGCTATGTTCGCTTTGGCTTAAATGATGCTTTATTGAAAACGTTACAGACATGGTGTAAAGGTAGAAGGATATTTTGCATGGTCATTGCTCGACGATTTTGAATGGACCGATGGATATACCGTGCGATTTGGCCTGATCTACATCGACCACAAGAACAACTTGACCAGGATACCAAAAGACTCACTAAAATGGTTCACCAATTTCCTCAAAACCTAACCTCTCTTTGGTTTTTTCTGCAGTATTTTGTTGTATGTTATTGCAAACTTCTAAAAGACTACTCGTAGAAAGAAGTAGCTAAATAAATTGCAATAACAAACCTTGTCTTATTTTATCA

mRNA sequence

ATGGGAAAATCTGCACCAGGCAGATGTTCCAAATGGCTACTTAATAACTGTTCGCATGGAGATTCCGCTACCGAACCATATATTGTTGGTCACCACCTCATTCTTGCTCATGCTCATGCAGTCGATATCTATCGCACGAAGTATCAGACGGTACAAAAGGGTGTGATTGGCATGACTGTGGTGGCACATTGGTTTGCCCCATATGATGATACCGAGGCAAATATAAAGGCTGCACGTCGAGCTACTGATTTCTTGTCTGGTTGGTACTTCAATCCAATCGTTCATGGTGATTATCCAGAAAGTATGATAATTCGTGTAAAAGATAGATTGCCAAGATTCACAATGAATGAGACCATTTTGATCAAAGGCAGCTATGATTTTCTTGGACTCAACTACTATACAGCTAGATATGCCATAGATAATTCCAATGAACATGTTCCATATCCATCCTACCTTACCGATTCTGGGGCAAATACTTCGCGTATGTGTTTAAGTGTGAGTGGATCTTGGCTTTCAGTTTATCCACAAGGATTACAATATACCTTGATGGACCTGAAATATAATTATAAGGATCCAGTTATCTGCATCACAGAAAATGGATATTTGGATCGCGATGGTCTTAATGTTTCTAAACTAATTCATGATACCAATAGAACGGATTACCATAGAGAACACCTCTATTATGTTCACAAAGCAATCGAACATGGTGTAAAGGTAGAAGGATATTTTGCATGGTCATTGCTCGACGATTTTGAATGGACCGATGGATATACCGTGCGATTTGGCCTGATCTACATCGACCACAAGAACAACTTGACCAGGATACCAAAAGACTCACTAAAATGGTTCACCAATTTCCTCAAAACCTAACCTCTCTTTGGTTTTTTCTGCAGTATTTTGTTGTATGTTATTGCAAACTTCTAAAAGACTACTCGTAGAAAGAAGTAGCTAAATAAATTGCAATAACAAACCTTGTCTTATTTTATCA

Coding sequence (CDS)

ATGGGAAAATCTGCACCAGGCAGATGTTCCAAATGGCTACTTAATAACTGTTCGCATGGAGATTCCGCTACCGAACCATATATTGTTGGTCACCACCTCATTCTTGCTCATGCTCATGCAGTCGATATCTATCGCACGAAGTATCAGACGGTACAAAAGGGTGTGATTGGCATGACTGTGGTGGCACATTGGTTTGCCCCATATGATGATACCGAGGCAAATATAAAGGCTGCACGTCGAGCTACTGATTTCTTGTCTGGTTGGTACTTCAATCCAATCGTTCATGGTGATTATCCAGAAAGTATGATAATTCGTGTAAAAGATAGATTGCCAAGATTCACAATGAATGAGACCATTTTGATCAAAGGCAGCTATGATTTTCTTGGACTCAACTACTATACAGCTAGATATGCCATAGATAATTCCAATGAACATGTTCCATATCCATCCTACCTTACCGATTCTGGGGCAAATACTTCGCGTATGTGTTTAAGTGTGAGTGGATCTTGGCTTTCAGTTTATCCACAAGGATTACAATATACCTTGATGGACCTGAAATATAATTATAAGGATCCAGTTATCTGCATCACAGAAAATGGATATTTGGATCGCGATGGTCTTAATGTTTCTAAACTAATTCATGATACCAATAGAACGGATTACCATAGAGAACACCTCTATTATGTTCACAAAGCAATCGAACATGGTGTAAAGGTAGAAGGATATTTTGCATGGTCATTGCTCGACGATTTTGAATGGACCGATGGATATACCGTGCGATTTGGCCTGATCTACATCGACCACAAGAACAACTTGACCAGGATACCAAAAGACTCACTAAAATGGTTCACCAATTTCCTCAAAACCTAA

Protein sequence

MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVVAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILIKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSVSGSWLSVYPQGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLKT

Homology

[Close]

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot
Match: Q5Z9Z0 (Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 9.9e-78
Identity = 139/294 (47.28%), Postives = 191/294 (64.97%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS    + CS GDS  EPYIV H+ +LAHA  V IYR KYQ  QKG IG+ +V
Sbjct: 209 GILAPGRCSSQGKSGCSKGDSGREPYIVAHNQLLAHAAVVQIYREKYQGGQKGKIGIAIV 268

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
           ++W  PY+D++ +  A +RA DF+ GW+ +P+  GDYP SM   V +RLPRFT  ++  I
Sbjct: 269 SNWMIPYEDSKEDKHATKRALDFMYGWFMDPLTKGDYPVSMRTLVGNRLPRFTKEQSKAI 328

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDS------GANTSRMCLSVSGSWLSVYP 181
            GS+DF+GLNYYTARY      +   + SY TDS        N + +      SWL +YP
Sbjct: 329 NGSFDFIGLNYYTARYIQGTKQDSNSHKSYSTDSLTNERVERNGTDIGPKAGSSWLYIYP 388

Query: 182 QGLQYTLMDLKYNYKDPVICITENGY--LDRDGLNVSKLIHDTNRTDYHREHLYYVHKAI 241
           +G++  L+  K  Y +P I ITENG   ++ + L++ + + DT R +++R+HL++V +A+
Sbjct: 389 KGIEELLLYTKRTYNNPTIYITENGVDEVNNENLSLKEALIDTTRIEFYRQHLFHVQRAL 448

Query: 242 EHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFL 288
             GV V GYFAWSL D+FEW DGY+VRFG+ YID+K+ L R PK S +W  NFL
Sbjct: 449 RQGVDVRGYFAWSLFDNFEWMDGYSVRFGINYIDYKDGLKRYPKRSSQWLQNFL 502

[Close]

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.4e-76
Identity = 137/294 (46.60%), Postives = 191/294 (64.97%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS W   NCS GDS  EPY   HH +LAHA  V +Y+ KYQ +QKG IG+T+V
Sbjct: 215 GLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLV 274

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
           +HWF P+  +++N  AA+RA DF+ GW+ +P++ GDYP SM   V +RLP+FT  ++ L+
Sbjct: 275 SHWFVPFSRSKSNNDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLV 334

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV------SGSWLSVYP 181
           KG++DF+GLNYYTA YA +    +    SY TDS AN + +   +      +  WL VYP
Sbjct: 335 KGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGVRNGIPIGPQAASPWLYVYP 394

Query: 182 QGLQYTLMDLKYNYKDPVICITENGY--LDRDGLNVSKLIHDTNRTDYHREHLYYVHKAI 241
           QG +  L+ +K NY +P + ITENG    +   L + + + D  R +Y+ +HL  +  AI
Sbjct: 395 QGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLPLQEALKDDARIEYYHKHLLSLLSAI 454

Query: 242 EHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFL 288
             G  V+GYFAWSLLD+FEW++GYTVRFG+ ++D+ +   R PK+S  WF  FL
Sbjct: 455 RDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVDYNDGRKRYPKNSAHWFKKFL 508

[Close]

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.4e-76
Identity = 137/294 (46.60%), Postives = 191/294 (64.97%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS W   NCS GDS  EPY   HH +LAHA  V +Y+ KYQ +QKG IG+T+V
Sbjct: 215 GLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLV 274

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
           +HWF P+  +++N  AA+RA DF+ GW+ +P++ GDYP SM   V +RLP+FT  ++ L+
Sbjct: 275 SHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLV 334

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV------SGSWLSVYP 181
           KG++DF+GLNYYTA YA +    +    SY TDS AN + +   +      +  WL VYP
Sbjct: 335 KGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGVRNGIPIGPQAASPWLYVYP 394

Query: 182 QGLQYTLMDLKYNYKDPVICITENGY--LDRDGLNVSKLIHDTNRTDYHREHLYYVHKAI 241
           QG +  L+ +K NY +P + ITENG    +   L + + + D  R +Y+ +HL  +  AI
Sbjct: 395 QGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLPLQEALKDDARIEYYHKHLLSLLSAI 454

Query: 242 EHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFL 288
             G  V+GYFAWSLLD+FEW++GYTVRFG+ ++D+ +   R PK+S  WF  FL
Sbjct: 455 RDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVDYNDGRKRYPKNSAHWFKKFL 508

[Close]

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot
Match: Q7XKV2 (Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 2.1e-75
Identity = 137/295 (46.44%), Postives = 192/295 (65.08%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS W   NCS GDS  EPY   HH +LAHA  V +Y+ KYQ +QKG IG+T+V
Sbjct: 212 GMFAPGRCSPW-EGNCSAGDSGREPYTACHHQLLAHAETVRLYKEKYQVLQKGKIGITLV 271

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
           ++WF P+  +++NI AARRA DF+ GW+ +P++ G+YP SM   V++RLP+FT  ++ LI
Sbjct: 272 SNWFVPFSRSKSNIDAARRALDFMLGWFMDPLIRGEYPLSMRELVRNRLPQFTKEQSELI 331

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV------SGSWLSVYP 181
           KGS+DF+GLNYYT+ YA      +    SY TD+ AN + +   +      +  WL +YP
Sbjct: 332 KGSFDFIGLNYYTSNYAGSLPPSNGLNNSYSTDARANLTAVRNGIPIGPQAASPWLYIYP 391

Query: 182 QGLQYTLMDLKYNYKDPVICITENGY--LDRDGLNVSKLIHDTNRTDYHREHLYYVHKAI 241
           QG +  ++ +K NY +P I ITENG    +   L + + + D  R DY+ +HL  +  AI
Sbjct: 392 QGFRELVLYVKENYGNPTIYITENGVDEFNNKTLPLQEALKDDTRIDYYHKHLLSLLSAI 451

Query: 242 EHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
             G  V+GYFAWSLLD+FEW++GYTVRFG+ ++D+ +   R PK S  WF  FL+
Sbjct: 452 RDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVDYNDGAKRYPKMSAHWFKEFLQ 505

[Close]

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot
Match: Q42707 (Furostanol glycoside 26-O-beta-glucosidase OS=Hellenia speciosa OX=49577 GN=F26G PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.0e-74
Identity = 141/294 (47.96%), Postives = 188/294 (63.95%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G+ APGRCS W    C  GDSA EPY V H+L+LAHA+AV IYR  Y+  Q G IG+T+ 
Sbjct: 272 GRHAPGRCSTWY--GCPAGDSANEPYEVTHNLLLAHANAVKIYRDNYKATQNGEIGITLN 331

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
           + W+ PY  +  +++AA RA DF+ GWY +P+V+GDYP  M   V+DRLP FT  E+ LI
Sbjct: 332 SLWYEPYSKSHEDVEAATRALDFMFGWYMDPLVNGDYPFIMRALVRDRLPFFTHAESELI 391

Query: 122 KGSYDFLGLNYYTARYA--IDNSNEHVP----YPSYLTDSGANTSRMCLSVSGSWLSVYP 181
           KGSYDF+G+NYYT+ YA     + +H P    + SY+  SG         + GSW+  YP
Sbjct: 392 KGSYDFIGINYYTSNYAQHAPVTEDHTPDNSYFDSYVNQSGEKNGVPIGPLQGSWIYFYP 451

Query: 182 QGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEH 241
           +GL+  L+ +K  Y +P I ITENG  + +       +HD  R +Y   HL  V +AI  
Sbjct: 452 RGLKELLLYVKRRYCNPKIYITENGTAEVE-KEKGVPLHDPERKEYLTYHLAQVLQAIRE 511

Query: 242 GVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLKT 290
           GV+V+G+F W+L D+FEW  GYT RFGLIYID+  +  R PKDS KWF+ FL+T
Sbjct: 512 GVRVKGHFTWALTDNFEWDKGYTERFGLIYIDYDKDFNRQPKDSTKWFSKFLRT 562

[Close]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL
Match: A0A6J1JKM7 (cyanogenic beta-glucosidase-like OS=Cucurbita maxima OX=3661 GN=LOC111485436 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.0e-109
Identity = 188/292 (64.38%), Postives = 230/292 (78.77%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS+W+LNNC  GDS+ EPYIVGH+ ILAHA AVD+YRTKYQ  QKGVIG+TV+
Sbjct: 211 GTFAPGRCSRWMLNNCCDGDSSKEPYIVGHNQILAHARAVDVYRTKYQRTQKGVIGITVI 270

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
            +W+ PY DT A+ +AA+RA +F SGW+FNPIVHGDYP+SM +RVK+RLP FT +E  LI
Sbjct: 271 GNWYEPYSDTCADEEAAKRAIEFFSGWFFNPIVHGDYPQSMRLRVKERLPTFTQDEATLI 330

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYPQ 181
           KGSYDFLGLNYYTA YAID+S+ HV YPS+LTDSG NTS   +S+     + SWLS+YP+
Sbjct: 331 KGSYDFLGLNYYTAAYAIDDSSAHVLYPSWLTDSGVNTS---ISLGPKVNASSWLSIYPE 390

Query: 182 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 241
           GL+  L+ +K +Y +P I ITENG+ D D  +  KL+ D  RT YH  HLY++H+A+  G
Sbjct: 391 GLKKLLITMKNDYNNPSIYITENGFFDYDDPSARKLLCDRKRTRYHMGHLYFLHEAMMDG 450

Query: 242 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           V+V GYFAWSLLD FEW+DGYT RFGL+YID  NNLTR PKDSLKWF+ FLK
Sbjct: 451 VRVNGYFAWSLLDTFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFSYFLK 499

[Close]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL
Match: A0A6J1H894 (beta-glucosidase 24-like OS=Cucurbita moschata OX=3662 GN=LOC111461391 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.1e-106
Identity = 187/292 (64.04%), Postives = 228/292 (78.08%), Query Frame = 0


Query: 1   MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTV 60
           +G+  PGRCS+W ++NC  G++ATEPYIVGH+LILAHAHAV +YR KYQ  QKGVIG+TV
Sbjct: 252 LGQYPPGRCSEWQIHNCLGGNAATEPYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITV 311

Query: 61  VAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 120
           +A W+ P  DT A+I+AA RA DF SGW+FNP+VHG+YPE+MI RVK+RLP FT  + IL
Sbjct: 312 LADWYVPLTDTPADIEAANRAVDFFSGWFFNPVVHGNYPETMISRVKERLPHFTEEQRIL 371

Query: 121 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGA---NTSRMCLSV-SGSWLSVYPQ 180
           IKGSYDFLG+NYYT+ YA  N ++    PSYLTDSGA   N   +   V + SWL+VYPQ
Sbjct: 372 IKGSYDFLGVNYYTSNYA-KNDDKPAERPSYLTDSGAISLNEPSLGPKVNASSWLAVYPQ 431

Query: 181 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 240
           GL+ TLMD+K  Y DP+I ITENGY D DG NV +LI D  R +++ +HLYY+HKA+E G
Sbjct: 432 GLKDTLMDIKRKYNDPIIYITENGYFDYDGPNVKELIDDKKRAEFYNDHLYYLHKAMEDG 491

Query: 241 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           VKV+G+FAW+LLDDFEW  GYT RFGLIYID  N+L RIPK SLKWF NFLK
Sbjct: 492 VKVKGFFAWTLLDDFEWQSGYTQRFGLIYIDFLNDLKRIPKLSLKWFANFLK 542

[Close]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL
Match: A0A6J1JG58 (beta-glucosidase 24-like OS=Cucurbita maxima OX=3661 GN=LOC111485420 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 4.0e-106
Identity = 187/292 (64.04%), Postives = 228/292 (78.08%), Query Frame = 0


Query: 1   MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTV 60
           +G+  PGRCS+W ++NC  G++ATEPYIVGH+LILAHAHAV +YR KYQ  QKGVIG+TV
Sbjct: 251 VGQYPPGRCSEWQIHNCLGGNAATEPYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITV 310

Query: 61  VAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 120
           +A W+ P  DT A+I+AA RA DF SGW+FNP+VHG+YPE+MI RVK+RLP+FT  + IL
Sbjct: 311 LADWYVPLTDTPADIEAANRAVDFFSGWFFNPVVHGNYPETMISRVKERLPQFTEEQRIL 370

Query: 121 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGA---NTSRMCLSV-SGSWLSVYPQ 180
           IKGSYDFLG+NYYT+ YA  N ++    PSYLTDSGA   N   +   V + SWL+VYP+
Sbjct: 371 IKGSYDFLGVNYYTSNYA-KNDDKPAQRPSYLTDSGAISLNEPSLGPKVNASSWLAVYPR 430

Query: 181 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 240
           GL+  LMD+K  Y DPVI ITENGY D DG N  +LI D  R +++ EHLYY+HKA+E G
Sbjct: 431 GLKDALMDIKRKYNDPVIYITENGYFDYDGPNFKELIDDKKRAEFYNEHLYYLHKAMEDG 490

Query: 241 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           VKV+G+FAW+LLDDFEW  GYT RFGLIYID  N+L RIPK SLKWFTNFLK
Sbjct: 491 VKVKGFFAWTLLDDFEWQSGYTERFGLIYIDFLNDLKRIPKLSLKWFTNFLK 541

[Close]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL
Match: A0A6J1HBR5 (beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461398 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 4.4e-105
Identity = 184/292 (63.01%), Postives = 225/292 (77.05%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS+W+LNNC  GDS+ EPYIVGH+ ILAHA AV +YRTKYQ  QKGVIG+TV+
Sbjct: 211 GTFAPGRCSRWILNNCYDGDSSKEPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVI 270

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
            +W+ PY +T A+ +AA+RA +F SGW+FNPIVHGDYP+SM +RVKDRLP FT +E  LI
Sbjct: 271 GNWYEPYSNTFADEEAAKRAIEFFSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLI 330

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYPQ 181
           K SYDFLGLNYYTA YAID+S+ HV YPS+ TDSG NTS   +S+     + SWLSVYP+
Sbjct: 331 KCSYDFLGLNYYTAAYAIDDSSAHVLYPSWRTDSGVNTS---ISLGPKVNASSWLSVYPK 390

Query: 182 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 241
           GLQ  L+ +K +Y +P I ITENG+ D D  +  KL+ D  R  YH  HLY++ +A+  G
Sbjct: 391 GLQKLLITMKNDYDNPSIYITENGFFDYDDPSARKLLCDRKRIFYHMGHLYFLRQAMRDG 450

Query: 242 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           V+V GYFAWS LD+FEW+DGYT RFGL+YID  NNLTR PKDSLKWF+ FLK
Sbjct: 451 VRVNGYFAWSFLDNFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFSLFLK 499

[Close]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL
Match: A0A6J1H9S5 (beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461398 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 4.4e-105
Identity = 184/292 (63.01%), Postives = 225/292 (77.05%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS+W+LNNC  GDS+ EPYIVGH+ ILAHA AV +YRTKYQ  QKGVIG+TV+
Sbjct: 114 GTFAPGRCSRWILNNCYDGDSSKEPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVI 173

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
            +W+ PY +T A+ +AA+RA +F SGW+FNPIVHGDYP+SM +RVKDRLP FT +E  LI
Sbjct: 174 GNWYEPYSNTFADEEAAKRAIEFFSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLI 233

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYPQ 181
           K SYDFLGLNYYTA YAID+S+ HV YPS+ TDSG NTS   +S+     + SWLSVYP+
Sbjct: 234 KCSYDFLGLNYYTAAYAIDDSSAHVLYPSWRTDSGVNTS---ISLGPKVNASSWLSVYPK 293

Query: 182 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 241
           GLQ  L+ +K +Y +P I ITENG+ D D  +  KL+ D  R  YH  HLY++ +A+  G
Sbjct: 294 GLQKLLITMKNDYDNPSIYITENGFFDYDDPSARKLLCDRKRIFYHMGHLYFLRQAMRDG 353

Query: 242 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           V+V GYFAWS LD+FEW+DGYT RFGL+YID  NNLTR PKDSLKWF+ FLK
Sbjct: 354 VRVNGYFAWSFLDNFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFSLFLK 402

[Close]

BLAST of Lcy03g009530 vs. NCBI nr
Match: XP_022988084.1 (cyanogenic beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 404.8 bits (1039), Expect = 6.1e-109
Identity = 188/292 (64.38%), Postives = 230/292 (78.77%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS+W+LNNC  GDS+ EPYIVGH+ ILAHA AVD+YRTKYQ  QKGVIG+TV+
Sbjct: 211 GTFAPGRCSRWMLNNCCDGDSSKEPYIVGHNQILAHARAVDVYRTKYQRTQKGVIGITVI 270

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
            +W+ PY DT A+ +AA+RA +F SGW+FNPIVHGDYP+SM +RVK+RLP FT +E  LI
Sbjct: 271 GNWYEPYSDTCADEEAAKRAIEFFSGWFFNPIVHGDYPQSMRLRVKERLPTFTQDEATLI 330

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYPQ 181
           KGSYDFLGLNYYTA YAID+S+ HV YPS+LTDSG NTS   +S+     + SWLS+YP+
Sbjct: 331 KGSYDFLGLNYYTAAYAIDDSSAHVLYPSWLTDSGVNTS---ISLGPKVNASSWLSIYPE 390

Query: 182 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 241
           GL+  L+ +K +Y +P I ITENG+ D D  +  KL+ D  RT YH  HLY++H+A+  G
Sbjct: 391 GLKKLLITMKNDYNNPSIYITENGFFDYDDPSARKLLCDRKRTRYHMGHLYFLHEAMMDG 450

Query: 242 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           V+V GYFAWSLLD FEW+DGYT RFGL+YID  NNLTR PKDSLKWF+ FLK
Sbjct: 451 VRVNGYFAWSLLDTFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFSYFLK 499

[Close]

BLAST of Lcy03g009530 vs. NCBI nr
Match: XP_038880765.1 (cyanogenic beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 401.4 bits (1030), Expect = 6.8e-108
Identity = 191/294 (64.97%), Postives = 229/294 (77.89%), Query Frame = 0


Query: 1   MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTV 60
           +G  APGRCS W+LNNC  G+S+TEPYIV HH ILAHAHAV +YR KYQ +QKG+IG+TV
Sbjct: 212 IGTFAPGRCSSWVLNNCCDGNSSTEPYIVSHHEILAHAHAVKLYRMKYQKIQKGIIGITV 271

Query: 61  VAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 120
           + +W+ PY ++ A+ +AA+RAT+F SGW++NPIVHGDYPESM +RVKDRLP FT  E  L
Sbjct: 272 LENWYEPYSESAADKEAAKRATEFFSGWFYNPIVHGDYPESMRLRVKDRLPTFTEEEKNL 331

Query: 121 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYP 180
           IKGS+DFLGLNYYTA YAID+SN HV YPSYLTDSG NTS   +S+     + SWLSVYP
Sbjct: 332 IKGSFDFLGLNYYTAAYAIDDSNTHVLYPSYLTDSGVNTS---ISLGPKLNASSWLSVYP 391

Query: 181 QGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAI-E 240
            GL+  L+ +K NY +P I ITENG+ D D  NV KLI D  RT YH  HLY++H+AI  
Sbjct: 392 PGLEKLLVTMKNNYNNPNIYITENGFFDYDDPNVGKLICDKKRTLYHITHLYHLHRAIFT 451

Query: 241 HGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           HGV+V+GYFAWSLLD FEW+DGYT RFGL+YID  NNL R PK S +WFT FLK
Sbjct: 452 HGVQVKGYFAWSLLDTFEWSDGYTKRFGLVYIDRSNNLKRTPKKSQEWFTYFLK 502

[Close]

BLAST of Lcy03g009530 vs. NCBI nr
Match: XP_022960671.1 (beta-glucosidase 24-like [Cucurbita moschata])

HSP 1 Score: 396.4 bits (1017), Expect = 2.2e-106
Identity = 187/292 (64.04%), Postives = 228/292 (78.08%), Query Frame = 0


Query: 1   MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTV 60
           +G+  PGRCS+W ++NC  G++ATEPYIVGH+LILAHAHAV +YR KYQ  QKGVIG+TV
Sbjct: 252 LGQYPPGRCSEWQIHNCLGGNAATEPYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITV 311

Query: 61  VAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 120
           +A W+ P  DT A+I+AA RA DF SGW+FNP+VHG+YPE+MI RVK+RLP FT  + IL
Sbjct: 312 LADWYVPLTDTPADIEAANRAVDFFSGWFFNPVVHGNYPETMISRVKERLPHFTEEQRIL 371

Query: 121 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGA---NTSRMCLSV-SGSWLSVYPQ 180
           IKGSYDFLG+NYYT+ YA  N ++    PSYLTDSGA   N   +   V + SWL+VYPQ
Sbjct: 372 IKGSYDFLGVNYYTSNYA-KNDDKPAERPSYLTDSGAISLNEPSLGPKVNASSWLAVYPQ 431

Query: 181 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 240
           GL+ TLMD+K  Y DP+I ITENGY D DG NV +LI D  R +++ +HLYY+HKA+E G
Sbjct: 432 GLKDTLMDIKRKYNDPIIYITENGYFDYDGPNVKELIDDKKRAEFYNDHLYYLHKAMEDG 491

Query: 241 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           VKV+G+FAW+LLDDFEW  GYT RFGLIYID  N+L RIPK SLKWF NFLK
Sbjct: 492 VKVKGFFAWTLLDDFEWQSGYTQRFGLIYIDFLNDLKRIPKLSLKWFANFLK 542

[Close]

BLAST of Lcy03g009530 vs. NCBI nr
Match: XP_022988066.1 (beta-glucosidase 24-like [Cucurbita maxima])

HSP 1 Score: 394.4 bits (1012), Expect = 8.3e-106
Identity = 187/292 (64.04%), Postives = 228/292 (78.08%), Query Frame = 0


Query: 1   MGKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTV 60
           +G+  PGRCS+W ++NC  G++ATEPYIVGH+LILAHAHAV +YR KYQ  QKGVIG+TV
Sbjct: 251 VGQYPPGRCSEWQIHNCLGGNAATEPYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITV 310

Query: 61  VAHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 120
           +A W+ P  DT A+I+AA RA DF SGW+FNP+VHG+YPE+MI RVK+RLP+FT  + IL
Sbjct: 311 LADWYVPLTDTPADIEAANRAVDFFSGWFFNPVVHGNYPETMISRVKERLPQFTEEQRIL 370

Query: 121 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGA---NTSRMCLSV-SGSWLSVYPQ 180
           IKGSYDFLG+NYYT+ YA  N ++    PSYLTDSGA   N   +   V + SWL+VYP+
Sbjct: 371 IKGSYDFLGVNYYTSNYA-KNDDKPAQRPSYLTDSGAISLNEPSLGPKVNASSWLAVYPR 430

Query: 181 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 240
           GL+  LMD+K  Y DPVI ITENGY D DG N  +LI D  R +++ EHLYY+HKA+E G
Sbjct: 431 GLKDALMDIKRKYNDPVIYITENGYFDYDGPNFKELIDDKKRAEFYNEHLYYLHKAMEDG 490

Query: 241 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           VKV+G+FAW+LLDDFEW  GYT RFGLIYID  N+L RIPK SLKWFTNFLK
Sbjct: 491 VKVKGFFAWTLLDDFEWQSGYTERFGLIYIDFLNDLKRIPKLSLKWFTNFLK 541

[Close]

BLAST of Lcy03g009530 vs. NCBI nr
Match: XP_023516697.1 (beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.0 bits (1011), Expect = 1.1e-105
Identity = 186/292 (63.70%), Postives = 226/292 (77.40%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCS+W+LNNC  GDS+ EPYIVGH+ ILAHA AV +YRTKYQ  QKGVIG+TV+
Sbjct: 211 GTFAPGRCSRWILNNCCDGDSSKEPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVI 270

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETILI 121
            +W+ PY +T A+ +AA+RA +F SGW+FNPIVHGDYP+SM +RVKDRLP FT +E  LI
Sbjct: 271 GNWYEPYSNTFADKEAAKRAIEFFSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLI 330

Query: 122 KGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSV-----SGSWLSVYPQ 181
           K SYDFLGLNYYTA YAID+S+ HV YPS+ TDSG NTS   +S+     + SWLSVYP+
Sbjct: 331 KCSYDFLGLNYYTAAYAIDDSSAHVLYPSWRTDSGVNTS---ISLGPKVNASSWLSVYPK 390

Query: 182 GLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKLIHDTNRTDYHREHLYYVHKAIEHG 241
           GLQ  L+ +K +Y +P I ITENG+ D D  +  KL+ D  RT YH  HLY++ +A+  G
Sbjct: 391 GLQKLLITMKNDYNNPSIYITENGFFDYDDPSARKLLCDRKRTFYHMGHLYFLLEAMRDG 450

Query: 242 VKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           V+V GYFAWSLLD FEW+DGYT RFGL+YID  NNLTR PKDSLKWF+ FLK
Sbjct: 451 VRVNGYFAWSLLDTFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFSYFLK 499

[Close]

BLAST of Lcy03g009530 vs. TAIR 10
Match: AT3G60130.1 (beta glucosidase 16 )

HSP 1 Score: 271.2 bits (692), Expect = 9.8e-73
Identity = 141/302 (46.69%), Postives = 190/302 (62.91%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G+ APGRCS +   +C  GD+ATEPYIVGH+L+LAH  AV +YR KYQ  QKG IG+ + 
Sbjct: 211 GQKAPGRCSNFYKPDCLGGDAATEPYIVGHNLLLAHGVAVKVYREKYQATQKGEIGIALN 270

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKD-RLPRFTMNETIL 121
             W  PY D+ A+  AA RAT F   ++  PIV+G YP  M+  VKD RLP FT  E+ +
Sbjct: 271 TAWHYPYSDSYADRLAATRATAFTFDYFMEPIVYGRYPIEMVSHVKDGRLPTFTPEESEM 330

Query: 122 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSVSG------------ 181
           +KGSYDF+G+NYY++ YA D     VP     T++   T+  C+S+ G            
Sbjct: 331 LKGSYDFIGVNYYSSLYAKD-----VP---CATENITMTTDSCVSLVGERNGVPIGPAAG 390

Query: 182 -SWLSVYPQGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKL-IHDTNRTDYHREHL 241
             WL +YP+G++  L+  K+ Y DPV+ ITENG    D  N+ K+ ++D  R DY+  HL
Sbjct: 391 SDWLLIYPKGIRDLLLHAKFRYNDPVLYITENGV---DEANIGKIFLNDDLRIDYYAHHL 450

Query: 242 YYVHKAIEHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNF 289
             V  AI  GV V+GYFAWSL+D+FEW++GYTVRFGL+++D ++   R  K S KWF   
Sbjct: 451 KMVSDAISIGVNVKGYFAWSLMDNFEWSEGYTVRFGLVFVDFEDGRKRYLKKSAKWFRRL 501

[Close]

BLAST of Lcy03g009530 vs. TAIR 10
Match: AT3G60130.2 (beta glucosidase 16 )

HSP 1 Score: 271.2 bits (692), Expect = 9.8e-73
Identity = 141/302 (46.69%), Postives = 190/302 (62.91%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G+ APGRCS +   +C  GD+ATEPYIVGH+L+LAH  AV +YR KYQ  QKG IG+ + 
Sbjct: 159 GQKAPGRCSNFYKPDCLGGDAATEPYIVGHNLLLAHGVAVKVYREKYQATQKGEIGIALN 218

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKD-RLPRFTMNETIL 121
             W  PY D+ A+  AA RAT F   ++  PIV+G YP  M+  VKD RLP FT  E+ +
Sbjct: 219 TAWHYPYSDSYADRLAATRATAFTFDYFMEPIVYGRYPIEMVSHVKDGRLPTFTPEESEM 278

Query: 122 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSVSG------------ 181
           +KGSYDF+G+NYY++ YA D     VP     T++   T+  C+S+ G            
Sbjct: 279 LKGSYDFIGVNYYSSLYAKD-----VP---CATENITMTTDSCVSLVGERNGVPIGPAAG 338

Query: 182 -SWLSVYPQGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKL-IHDTNRTDYHREHL 241
             WL +YP+G++  L+  K+ Y DPV+ ITENG    D  N+ K+ ++D  R DY+  HL
Sbjct: 339 SDWLLIYPKGIRDLLLHAKFRYNDPVLYITENGV---DEANIGKIFLNDDLRIDYYAHHL 398

Query: 242 YYVHKAIEHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNF 289
             V  AI  GV V+GYFAWSL+D+FEW++GYTVRFGL+++D ++   R  K S KWF   
Sbjct: 399 KMVSDAISIGVNVKGYFAWSLMDNFEWSEGYTVRFGLVFVDFEDGRKRYLKKSAKWFRRL 449

[Close]

BLAST of Lcy03g009530 vs. TAIR 10
Match: AT3G60130.3 (beta glucosidase 16 )

HSP 1 Score: 265.4 bits (677), Expect = 5.4e-71
Identity = 138/291 (47.42%), Postives = 188/291 (64.60%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G+ APGRCS +   +C  GD+ATEPYIVGH+L+LAH  AV +YR KYQ  QKG IG+ + 
Sbjct: 159 GQKAPGRCSNFYKPDCLGGDAATEPYIVGHNLLLAHGVAVKVYREKYQATQKGEIGIALN 218

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKD-RLPRFTMNETIL 121
             W  PY D+ A+  AA RAT F   ++  PIV+G YP  M+  VKD RLP FT  E+ +
Sbjct: 219 TAWHYPYSDSYADRLAATRATAFTFDYFMEPIVYGRYPIEMVSHVKDGRLPTFTPEESEM 278

Query: 122 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSVSG--SWLSVYPQGL 181
           +KGSYDF+G+NYY++ YA D     VP     T++   T+  C+S+ G  + + + P G+
Sbjct: 279 LKGSYDFIGVNYYSSLYAKD-----VP---CATENITMTTDSCVSLVGERNGVPIGPAGI 338

Query: 182 QYTLMDLKYNYKDPVICITENGYLDRDGLNVSKL-IHDTNRTDYHREHLYYVHKAIEHGV 241
           +  L+  K+ Y DPV+ ITENG    D  N+ K+ ++D  R DY+  HL  V  AI  GV
Sbjct: 339 RDLLLHAKFRYNDPVLYITENGV---DEANIGKIFLNDDLRIDYYAHHLKMVSDAISIGV 398

Query: 242 KVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
            V+GYFAWSL+D+FEW++GYTVRFGL+++D ++   R  K S KWF   LK
Sbjct: 399 NVKGYFAWSLMDNFEWSEGYTVRFGLVFVDFEDGRKRYLKKSAKWFRRLLK 438

[Close]

BLAST of Lcy03g009530 vs. TAIR 10
Match: AT5G44640.1 (beta glucosidase 13 )

HSP 1 Score: 263.8 bits (673), Expect = 1.6e-70
Identity = 138/301 (45.85%), Postives = 185/301 (61.46%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  APGRCSK+   NC+ G+ ATEPYIVGH+LILAH  AV +YR KY+  QKG +G+ + 
Sbjct: 212 GVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLILAHGEAVKVYREKYKASQKGQVGIALN 271

Query: 62  AHWFAPYDDTEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKD-RLPRFTMNETIL 121
           A W  PY ++  +  AA RA  F   ++  P+V G YP  M+  VKD RLP FT  ++ +
Sbjct: 272 AGWNLPYTESAEDRLAAARAMAFTFDYFMEPLVTGKYPVDMVNNVKDGRLPTFTAKQSKM 331

Query: 122 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSGANTSRMCLSVSG------------ 181
           +KGSYDF+G+NYY++ YA D     VP  S   ++    S  C SV+G            
Sbjct: 332 LKGSYDFIGINYYSSSYAKD-----VPCSS---ENVTLFSDPCASVTGEREGVPIGPKAA 391

Query: 182 -SWLSVYPQGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSKL-IHDTNRTDYHREHL 241
             WL +YP+G++  L+  KY +KDPV+ ITENG   RD  +  K+ + D+ R DY+ +HL
Sbjct: 392 SDWLLIYPKGIRDLLLYAKYKFKDPVMYITENG---RDEASTGKIDLKDSERIDYYAQHL 451

Query: 242 YYVHKAIEHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNF 288
             V  AI  G  V+G+FAWSLLD+FEW  GY+VRFGL+Y+D  +   R PK S KWF   
Sbjct: 452 KMVQDAISIGANVKGFFAWSLLDNFEWATGYSVRFGLVYVDFNDGRKRYPKKSAKWFRKL 501

[Close]

BLAST of Lcy03g009530 vs. TAIR 10
Match: AT5G24540.1 (beta glucosidase 31 )

HSP 1 Score: 262.3 bits (669), Expect = 4.6e-70
Identity = 131/299 (43.81%), Postives = 187/299 (62.54%), Query Frame = 0


Query: 2   GKSAPGRCSKWLLNNCSHGDSATEPYIVGHHLILAHAHAVDIYRTKYQTVQKGVIGMTVV 61
           G  A GRCSKW+ + C  GDS TEPY+V HHL+LAHA AV+ +R   +  Q   IG+ + 
Sbjct: 212 GNKAMGRCSKWVNSLCIAGDSGTEPYLVSHHLLLAHAAAVEEFRKCDKISQDSKIGIVLS 271

Query: 62  AHWFAPYDD-TEANIKAARRATDFLSGWYFNPIVHGDYPESMIIRVKDRLPRFTMNETIL 121
            +WF PYD  + A+ +A  RA  F  GW+ +P+V GDYPE++ I   +RLP FT  ++++
Sbjct: 272 PYWFEPYDSASNADKEAVERALAFNIGWHLSPLVFGDYPETIKISAGNRLPSFTKEQSMM 331

Query: 122 IKGSYDFLGLNYYTARYAIDNSNEHVPYPSYLTDSG-----ANTSRMCLSVSGSWLSV-- 181
           +K S+DF+G+NYYTAR+   + N  +  P ++TD        N +   +S+      +  
Sbjct: 332 VKNSFDFIGVNYYTARFVAHDLNVDISRPRFMTDQHLQYKLTNRTGDTISLESDGTKILW 391

Query: 182 -YPQGLQYTLMDLKYNYKDPVICITENGYLDRDGLNVSK--LIHDTNRTDYHREHLYYVH 241
            YP+GL+  L  +K  Y +P I ITENG+ D +   V++  ++ DT R +YH++HL  + 
Sbjct: 392 SYPEGLRKILNYIKNKYNNPTIYITENGFDDYENGTVTREEILEDTKRIEYHQKHLQELQ 451

Query: 242 KAI-EHGVKVEGYFAWSLLDDFEWTDGYTVRFGLIYIDHKNNLTRIPKDSLKWFTNFLK 289
           KAI E G  V+GYF WSLLD+FEW  GY VRFGL Y+D+KN L R  K S  WF +FL+
Sbjct: 452 KAITEDGCDVKGYFTWSLLDNFEWEHGYAVRFGLYYVDYKNGLQRHAKHSAMWFKHFLE 510

The following BLAST results are available for this feature:

BLAST of Lcy03g009530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z9Z0	9.9e-78	47.28	Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1	[more]
B8AVF0	2.4e-76	46.60	Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1	[more]
Q7XKV4	2.4e-76	46.60	Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2	[more]
Q7XKV2	2.1e-75	46.44	Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2	[more]
Q42707	1.0e-74	47.96	Furostanol glycoside 26-O-beta-glucosidase OS=Hellenia speciosa OX=49577 GN=F26G...	[more]

BLAST of Lcy03g009530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKM7	3.0e-109	64.38	cyanogenic beta-glucosidase-like OS=Cucurbita maxima OX=3661 GN=LOC111485436 PE=...	[more]
A0A6J1H894	1.1e-106	64.04	beta-glucosidase 24-like OS=Cucurbita moschata OX=3662 GN=LOC111461391 PE=3 SV=1	[more]
A0A6J1JG58	4.0e-106	64.04	beta-glucosidase 24-like OS=Cucurbita maxima OX=3661 GN=LOC111485420 PE=3 SV=1	[more]
A0A6J1HBR5	4.4e-105	63.01	beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146139...	[more]
A0A6J1H9S5	4.4e-105	63.01	beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11146139...	[more]

BLAST of Lcy03g009530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988084.1	6.1e-109	64.38	cyanogenic beta-glucosidase-like [Cucurbita maxima]	[more]
XP_038880765.1	6.8e-108	64.97	cyanogenic beta-glucosidase-like [Benincasa hispida]	[more]
XP_022960671.1	2.2e-106	64.04	beta-glucosidase 24-like [Cucurbita moschata]	[more]
XP_022988066.1	8.3e-106	64.04	beta-glucosidase 24-like [Cucurbita maxima]	[more]
XP_023516697.1	1.1e-105	63.70	beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy03g009530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60130.1	9.8e-73	46.69	beta glucosidase 16	[more]
AT3G60130.2	9.8e-73	46.69	beta glucosidase 16	[more]
AT3G60130.3	5.4e-71	47.42	beta glucosidase 16	[more]
AT5G44640.1	1.6e-70	45.85	beta glucosidase 13	[more]
AT5G24540.1	4.6e-70	43.81	beta glucosidase 31	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001360	Glycoside hydrolase family 1	PRINTS	PR00131	GLHYDRLASE1	coord: 194..202 score: 54.22 coord: 124..138 score: 49.51 coord: 215..226 score: 44.0 coord: 260..272 score: 43.79 coord: 236..253 score: 58.07
IPR001360	Glycoside hydrolase family 1	PFAM	PF00232	Glyco_hydro_1	coord: 20..288 e-value: 1.3E-72 score: 244.6
IPR001360	Glycoside hydrolase family 1	PANTHER	PTHR10353	GLYCOSYL HYDROLASE	coord: 2..288
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..289 e-value: 3.4E-105 score: 354.2
None	No IPR available	PANTHER	PTHR10353:SF137	BETA-GLUCOSIDASE 31-RELATED	coord: 2..288
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..288

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g009530.1	Lcy03g009530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds