Lcy03g008340 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g008340
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGlucan endo-1,3-beta-glucosidase 3
LocationChr03: 40384281 .. 40389507 (-)
RNA-Seq ExpressionLcy03g008340
SyntenyLcy03g008340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTACACCTTCAGTGTCGATATTATTTTCAATATCACAAGCTCAGTCCTGTTTAGTACAAAATATATACGTTGGGAAATGGTGGGGTATGATTCTTGTTGGAGGGTTGTTTTGTTTTGGGATTAATTTTTATTTGTGACTATTGATTTTCGCTCTCGAGGGAGTTTGGTTTTTTGCTCTTCTGTCATTTACTCTCGTTTCTGAGGCGTTTTACTGTTCTGGGCTGTTGAGAATCTCTCTTCTACACTTCTCTATTTCGTCCTTCTTCCCTCTTTCAACTTCCATTAATCTGTGTGCACTTGAGTGTTTACTCTCTCTTTAGGGTTTTGGTTCTGTTCTGAAGCGGACTGAAGACTGTTGGAATTGCAGTGTGTTCTGATAAATTATTCAGATCGGGTACTTGCCCACTTCTTGTTTTTAGTTTTCTCAGGTGGGTTCTGTGCCATTTCGTGTTTTGTGGCTGATTTTTCAAGTTTGGCTTTCCAGTAGTTCATAGTCTGCAGAAGTAGAATGAGAAAAAGGGGTTTCTCTTTTTGATGATATGTGGAAGCTAAGCTAAGCTTAGCTGCAGTTAGTTTGAAATTCTCGAATCTTTCTCTGAGATGGGGATTTAAAGAAAGAGTCTCATTTGTAAGCTAAGCTACAGTGTGTTTGTTAATGCTATGTCTGCATATTTATGGCTTTATTGCATTTGTCTCCTGTCTGAGGATCTGCAGAAAGAGCTTGGTTTGTAATATTTTGGGTGTGGAAAGAATTTTCTTTTCCAAACTTAACATTTCAAAAACAGAGATGTGGGTGCATTTCCATTTTGATTTCCCTCCTTTTAAGTTCAAAATTTCTGCTGAACCTATATGAAACACAAATAGATTAGATGTTTGCTAATTACAAGTTGGAAGTGAGGGGAGGAGGAAGCAATCTGTCATCTTGCAATGTTATCCTTACTTTTCTGTCTTTAATTGAATTATCTTTTCTATCACTCCCCTGTCATTGCAGTTTTTCCTTCACATTCAATTAGTCTTACACGTTTCTCTTGTTGGTTTTTGTTTATGTTTTCCTTTTCATATGACTACTTTGCAGATTTTATGTGCTTGCTTGTTTGAGTTTTGGGAGTTTGATTCTCGAGACGCAAAGGAAGTAGCAGGAAGCTAAAATCACACCAAGCTGAAAAGTTGAAGTTTTGGTTGATTGGAAAATGCGTGAAAGGTGTCATGGAATTGTGATCTTGCTTCTATTTGGCATGTGCGTCAATGCATTAGGTATCTTGGTTATCGAACTTTTAGTTCTTTAACATCCTTTACAGTTTCAGTTCTAAATGCAATTTGCTTGGAATGTTCTGAAAATTGCCATGAAATTTGTTTCCATGATCTCAATTGGTGGTTGTGATGTGAGCATAAGCAGTAAAAGTAAGGGCTTCATAAATGTGAATCAAATGGCCTCTTCAATAGTTTTGTGCAACCAACCTTCATTAGTTACTATCGATCTTTTCCCAACGATGAATGCATTGTTTGATTATAACTGATCATCTGTTTCTTACTAAGCTGTGTTCTTGGCATTAATAGGTGCATTTGTGGGGGTTAACCTTGGGACCGACGTCTCCAATCTTCCATCAGCTTCGGACATAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATTCTCAACTGCTAAAGGCCCTAGCAAACAGTAGCATTGAAGTAATTGTTGGTGTCACTAATGAGGAAGTTCTCAGGATCGGTGAATCTCCAGCAGCCGCTGCAGCCTGGGTTAATAAAAATGTTGCTGCTTACTTGCCAGGAACGAATATTACCGCCATTGCTGTTGGTAGTGAGGTCCTTACAACAATTCCTCATGTTGGTCCAGTCTTAGTTCCAGCCATGTATTCCCTACATAAGGCCTTGGTTGCTGCCAATCTAAACTATCTGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGGCCATTCCCCCCGTCCACGGCCGCCTTTAATGCTTCATGGAACTCTACGATCTATCAGCTCCTCCAGTTTTTAAAAAACACAAAGTCCTACTACATGTTGAATGCCTATCCTTATTATGGGTACACAAGTGGGAATGGAATTTTTCCTCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGACCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTATTCAATCGATGCATTCAATTTTTCTGGAATTCCCGTCGTTGTTACCGAGACTGGCTGGCCATCATTTGGTGGAGCAAATGAACCTGATGCTACAATTCAAAATGCTGGCACCTACATTAACAATCTGATCAGGAGAGTTTCCAATGATTCAGGTCCACCTAGTCAACCAACCATACCAATCAACACGTACCTTTACGAGTTGTTTAATGAAGACAAGAGGCCTGGTCCGGTCTCGGAAAAAAACTGGGGTATACTTTTCCCCAATGGCTCTACTGTTTATCCTTTGGGTTTGGCTTCTGGACGTGCAACAGCAAATTCTTCTGTAGTTTATTGTGTGGCTAAAGAAGGTGCCGATGAAGATAAGTTGCAAGACGGCCTGAATTGGGCTTGTGGACAAGGAGGGGCAAACTGTGTTGCCATTCAGCAAGGGAGGCCATGCTTTCTCCCCAATAACATTACTAACCATGCTTCTTATGCATACAATGATTACTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGATGGCACCGCTATGCTTACGACGAACGATCCCAGTAAGTTCTTTTAATAGTTTTTCTCCATGTTCCTTAGATTCAGTAAGCAGATGCTATAGTCCTACCTTATAAATTTTTTTGATGTGTTTTCGAAATCCAACATCTACCGAAATAAAAATGTATTTAAATCTAGAACTTTACCTATTCACTGGTTAACGTCCTGCAAAAGGATGGCTCATACAAACTCCTATTACTAATCCATTTTGGAACTAACATAAAGGAAAGAGAACTTAGAATCATGTGTGGGAAAGATATAAGACAGTTTTCCTTGAAGCAAAAACTTACTGGACCAAATTATTTGTTGGTTATTACTTTAGTATTTACTTCACACCTCTTTCTTTAGTCATAGACTTACTTCCCCTCTTAGAAGCAGACACTTTAGTTTGGCTAGCGTGTCCGTGTCTGACACGTGTCGGACACTTGGACACTCCCAACACTTGGACACGTATCGGACACTTATTAGCTCAATTAATATGTGGCAAACAATAGTTGTAGGTTCATATCGAACACTTGTTAAGTATACTAAATAAACACAATACTTAAATGACAAAAATAATAAATTTTAAGAGTAAAATATATTAAACTCATTTTTTTAAGCATACAAATGCATAAACTTGTTGATTTTGAATTTCTTTCTTGTATAAAAAACAATATATATTTTAAAAAATTTATATTTTTTTAAACGTGTTCGTTCCATGACTGTATCCTAGATTTAAAAAAAGTAACGTGTCACAATGTCCGTGTTGTGTCATATCCGTGTCTTGTGTCCGTGTCCGTGCTTCTTAGTACAAGGGGAAGTGTAGTAGTTGGAGGTCCTTCTATAACTTTCTTGCCTTCGGCTGAGTTTTTCCCCTCGTCCCTTTTGTAAACTTCAATCTTTTGAACAAGAGACAAACCTTTTTTTTATTTTTTATTTAATTTGTTTTAATTTTTTTTTTATTATTATTTTTTACTTGTTTACCTCAGTGAAATTGTCTCTTTTCCAAAATAAAAGAACAGAAAACTTAAATAGCGTGGTTGAAAAGCTTTGTATGGCCCTCATCAACTCCATATCTTTAAATTCTTAAAAGATTATATGGTTTCTGTCCAATCAAATGGTACTACAAAAAGAGTTTTTCCCACATTTAACCAGTAGAAGTTTGCCTTTACTCTTGAGCCCATGGCCACATGAAGTTCCTCTTTGATCTCCTTAACATTCTTAGAATAGCACCTCTGAATCTGATCCTTGATTGAGCCTGAGCCTCTGAATCTGATGCTTATATTTTAAAGATCTATTATTCTGTTGAGCACACTACATTGGATTTTGGTTTCTCTACCACGTTATCGAAGTTCTCCGAAAGCCTCGGGGTACTGCAATGTTTTCTGCAGAACATTACAAACATAAACTCTCTTCTGTTTCTCTTTAGTCCTTTTCTAGTTTTGTGCTTGTCCTATTCTTTTGGTATTAAGAATCTGATACCTTTGCTCAATTTGATTTATACAGCTCGTATTCACAGTTACATCGCTTGTATTATATCGTATATAAGATTCGTCTACCATTCTGTTTGCATACCCTGCCAAATCACAGGGAGGGGACTAGTTTTCTTATGTCAGCATTAAACTTGTGCTTACTTTTGTCTGTAATGCCTGTTTGAATGACTCCATCTGACATCGACTATTCGGGTTTGTACGGGATTCAACTTCAACTAAAAACTTTTATCCTTTTAATCCAATTTCTCTCATTTGGTTTGTTCCCCATCTTTTGCAGGTCATGGCTCTTGCATATACACAGGAAGGTAAGTGTAAACAGTTTGAATAACTTAAGAAAATCTTCTGTTTGTATATCAATACCCGACTTCTAAACTGTTCAAAATATTGAAAAACAGTTCCAATTCAAGCAGTGGAGGTGGATTTTCTCCTCCAACAGCATTGGGGCCTTCAGGTCTGCTTCCTGGTTCAAGTTCAAAGCTGCAGGTCTCTTCATTTCAGCTCTTGATTTTGGTTATCTTTTCATGGGCTTTGATGTTTACTTTGACATGAAAGCTTCTTCTTGAAGTTCAATAGCCAAACCATTCTTCAGATTGCTAATTTATTGAGCACAATGAAGAGAGATAGGATGATCTCATCAATACATGGTTTTAAATGTTCATCACTTTTCACTTTTTTATTCTTTTTACTCCCCCTCTTATTCCCCAATGTTTTTCAGTTCTAGATGCTCTGTTTATTATTGTTCATTGTAATTTTGTAATTTACTCTTCTGAGGTGGTAGTAACTGGGCTTAGTTTTTTGATTAATTTATATTATTTTTGAAATTAATTGTTTCCATTTTTGGTTATGGAGGGAAACTTATGAAAAAACAATGGTTGATCTATATATACTTTTTGACCATATGTGAGGTGAGAGATTTGAACTATAGACGTTTTGGTCATTAGTACAAATTTT

mRNA sequence

GTTTTTACACCTTCAGTGTCGATATTATTTTCAATATCACAAGCTCAGTCCTGTTTAGTACAAAATATATACGTTGGGAAATGGTGGGGTATGATTCTTGTTGGAGGGTTGTTTTGTTTTGGGATTAATTTTTATTTGTGACTATTGATTTTCGCTCTCGAGGGAGTTTGGTTTTTTGCTCTTCTGTCATTTACTCTCGTTTCTGAGGCGTTTTACTGTTCTGGGCTGTTGAGAATCTCTCTTCTACACTTCTCTATTTCGTCCTTCTTCCCTCTTTCAACTTCCATTAATCTGTGTGCACTTGAGTGTTTACTCTCTCTTTAGGGTTTTGGTTCTGTTCTGAAGCGGACTGAAGACTGTTGGAATTGCAGTGTGTTCTGATAAATTATTCAGATCGGGTACTTGCCCACTTCTTGTTTTTAGTTTTCTCAGATTTTATGTGCTTGCTTGTTTGAGTTTTGGGAGTTTGATTCTCGAGACGCAAAGGAAGTAGCAGGAAGCTAAAATCACACCAAGCTGAAAAGTTGAAGTTTTGGTTGATTGGAAAATGCGTGAAAGGTGTCATGGAATTGTGATCTTGCTTCTATTTGGCATGTGCGTCAATGCATTAGGTGCATTTGTGGGGGTTAACCTTGGGACCGACGTCTCCAATCTTCCATCAGCTTCGGACATAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATTCTCAACTGCTAAAGGCCCTAGCAAACAGTAGCATTGAAGTAATTGTTGGTGTCACTAATGAGGAAGTTCTCAGGATCGGTGAATCTCCAGCAGCCGCTGCAGCCTGGGTTAATAAAAATGTTGCTGCTTACTTGCCAGGAACGAATATTACCGCCATTGCTGTTGGTAGTGAGGTCCTTACAACAATTCCTCATGTTGGTCCAGTCTTAGTTCCAGCCATGTATTCCCTACATAAGGCCTTGGTTGCTGCCAATCTAAACTATCTGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGGCCATTCCCCCCGTCCACGGCCGCCTTTAATGCTTCATGGAACTCTACGATCTATCAGCTCCTCCAGTTTTTAAAAAACACAAAGTCCTACTACATGTTGAATGCCTATCCTTATTATGGGTACACAAGTGGGAATGGAATTTTTCCTCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGACCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTATTCAATCGATGCATTCAATTTTTCTGGAATTCCCGTCGTTGTTACCGAGACTGGCTGGCCATCATTTGGTGGAGCAAATGAACCTGATGCTACAATTCAAAATGCTGGCACCTACATTAACAATCTGATCAGGAGAGTTTCCAATGATTCAGGTCCACCTAGTCAACCAACCATACCAATCAACACGTACCTTTACGAGTTGTTTAATGAAGACAAGAGGCCTGGTCCGGTCTCGGAAAAAAACTGGGGTATACTTTTCCCCAATGGCTCTACTGTTTATCCTTTGGGTTTGGCTTCTGGACGTGCAACAGCAAATTCTTCTGTAGTTTATTGTGTGGCTAAAGAAGGTGCCGATGAAGATAAGTTGCAAGACGGCCTGAATTGGGCTTGTGGACAAGGAGGGGCAAACTGTGTTGCCATTCAGCAAGGGAGGCCATGCTTTCTCCCCAATAACATTACTAACCATGCTTCTTATGCATACAATGATTACTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGATGGCACCGCTATGCTTACGACGAACGATCCCAGTCATGGCTCTTGCATATACACAGGAAGTTCCAATTCAAGCAGTGGAGGTGGATTTTCTCCTCCAACAGCATTGGGGCCTTCAGGTCTGCTTCCTGGTTCAAGTTCAAAGCTGCAGGTCTCTTCATTTCAGCTCTTGATTTTGGTTATCTTTTCATGGGCTTTGATGTTTACTTTGACATGAAAGCTTCTTCTTGAAGTTCAATAGCCAAACCATTCTTCAGATTGCTAATTTATTGAGCACAATGAAGAGAGATAGGATGATCTCATCAATACATGGTTTTAAATGTTCATCACTTTTCACTTTTTTATTCTTTTTACTCCCCCTCTTATTCCCCAATGTTTTTCAGTTCTAGATGCTCTGTTTATTATTGTTCATTGTAATTTTGTAATTTACTCTTCTGAGGTGGTAGTAACTGGGCTTAGTTTTTTGATTAATTTATATTATTTTTGAAATTAATTGTTTCCATTTTTGGTTATGGAGGGAAACTTATGAAAAAACAATGGTTGATCTATATATACTTTTTGACCATATGTGAGGTGAGAGATTTGAACTATAGACGTTTTGGTCATTAGTACAAATTTT

Coding sequence (CDS)

ATGCGTGAAAGGTGTCATGGAATTGTGATCTTGCTTCTATTTGGCATGTGCGTCAATGCATTAGGTGCATTTGTGGGGGTTAACCTTGGGACCGACGTCTCCAATCTTCCATCAGCTTCGGACATAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATTCTCAACTGCTAAAGGCCCTAGCAAACAGTAGCATTGAAGTAATTGTTGGTGTCACTAATGAGGAAGTTCTCAGGATCGGTGAATCTCCAGCAGCCGCTGCAGCCTGGGTTAATAAAAATGTTGCTGCTTACTTGCCAGGAACGAATATTACCGCCATTGCTGTTGGTAGTGAGGTCCTTACAACAATTCCTCATGTTGGTCCAGTCTTAGTTCCAGCCATGTATTCCCTACATAAGGCCTTGGTTGCTGCCAATCTAAACTATCTGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGGCCATTCCCCCCGTCCACGGCCGCCTTTAATGCTTCATGGAACTCTACGATCTATCAGCTCCTCCAGTTTTTAAAAAACACAAAGTCCTACTACATGTTGAATGCCTATCCTTATTATGGGTACACAAGTGGGAATGGAATTTTTCCTCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGACCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTATTCAATCGATGCATTCAATTTTTCTGGAATTCCCGTCGTTGTTACCGAGACTGGCTGGCCATCATTTGGTGGAGCAAATGAACCTGATGCTACAATTCAAAATGCTGGCACCTACATTAACAATCTGATCAGGAGAGTTTCCAATGATTCAGGTCCACCTAGTCAACCAACCATACCAATCAACACGTACCTTTACGAGTTGTTTAATGAAGACAAGAGGCCTGGTCCGGTCTCGGAAAAAAACTGGGGTATACTTTTCCCCAATGGCTCTACTGTTTATCCTTTGGGTTTGGCTTCTGGACGTGCAACAGCAAATTCTTCTGTAGTTTATTGTGTGGCTAAAGAAGGTGCCGATGAAGATAAGTTGCAAGACGGCCTGAATTGGGCTTGTGGACAAGGAGGGGCAAACTGTGTTGCCATTCAGCAAGGGAGGCCATGCTTTCTCCCCAATAACATTACTAACCATGCTTCTTATGCATACAATGATTACTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGATGGCACCGCTATGCTTACGACGAACGATCCCAGTCATGGCTCTTGCATATACACAGGAAGTTCCAATTCAAGCAGTGGAGGTGGATTTTCTCCTCCAACAGCATTGGGGCCTTCAGGTCTGCTTCCTGGTTCAAGTTCAAAGCTGCAGGTCTCTTCATTTCAGCTCTTGATTTTGGTTATCTTTTCATGGGCTTTGATGTTTACTTTGACATGA

Protein sequence

MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYCVAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQLLILVIFSWALMFTLT
Homology
BLAST of Lcy03g008340 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.3e-191
Identity = 327/487 (67.15%), Postives = 405/487 (83.16%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           +++LL    C NA  AF+GVN+GTD++N+P  SDIV +LKS QITH+RLY+A+S +LKA 
Sbjct: 11  LLLLLSILACSNA--AFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAF 70

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           AN+SIEV+VGVTNEE+L+IG  P+AAAAWVNKNVAAY+P TNITAIAVGSEVLTTIPHV 
Sbjct: 71  ANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVA 130

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
           P+L  A+ ++HKALVA+NLN+ +KVS+P SMDI+P+PFPPST+ F+ SWN+T+YQLLQFL
Sbjct: 131 PILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFL 190

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           KNT S++MLNAYPYYGYT+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA 
Sbjct: 191 KNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAA 250

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           YYS++A NFS IPVVVTETGWPS GG++E  AT+ NA T+  NLI+RV N+SGPPSQP I
Sbjct: 251 YYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDI 310

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATA--NSSVVYCVAKEG 367
           PINTY+YEL+NEDKR GPVSE+NWGILFPNG++VYPL L+ G ++A  N S ++CVAK  
Sbjct: 311 PINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKAD 370

Query: 368 ADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDF 427
           AD+DKL DGLNWACGQG ANC AIQ G+PC+LPN++ +HAS+A+NDYYQKM SAGGTCDF
Sbjct: 371 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDF 430

Query: 428 DGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQLLILV 487
           DGTA+ TT DPS+ +C YTGS N+++  G  PP ALGP+  L G+++   + S+ L IL 
Sbjct: 431 DGTAITTTRDPSYRTCAYTGSLNANATNGNFPPDALGPASPLGGNANARIIFSYHLPILA 490

Query: 488 IFSWALM 493
             +  L+
Sbjct: 491 PLALTLL 495

BLAST of Lcy03g008340 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 459.1 bits (1180), Expect = 6.1e-128
Identity = 240/496 (48.39%), Postives = 330/496 (66.53%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           ++ L LF     +  + +GVN+GT+V+N+PS + +VA+LKS  I  +RLY+AD  +L A 
Sbjct: 6   LLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAF 65

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           A++ ++VI+ V N+++L I +S A AA WV +NVAAY P TNIT IAVGSEVLT++ +  
Sbjct: 66  AHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAA 125

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
            VLV A+  +  ALV ANL+  IKVSTP S  II   FPPS A FN +W+  I  LL+FL
Sbjct: 126 SVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFL 185

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           ++T S  +LN YPY+ Y   NG+ PLDYALF+ L A K+ VD NTL HY ++FDA+VDA 
Sbjct: 186 QSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAA 245

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           Y+++   NF+ IP+VVTE+GWPS GG +E DAT++NA TY +NLI+ V N +G P  P  
Sbjct: 246 YFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGT 305

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLA-SGRATANSSV--VYCVAKE 367
            + TY+YEL+NED RPGPVSEKNWG+ + NG+ VY L LA +G   AN +    +C+AKE
Sbjct: 306 AVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKE 365

Query: 368 GADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCD 427
             D   LQ  L+WACG G  +C A+ QG  C+ P+++  H++YA+N YYQKM  A G+CD
Sbjct: 366 KVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCD 425

Query: 428 FDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGP------SGLLP-------GSS 487
           F G A +TT DPS G+C++ GS+ S+   G +  +AL P      SG +P        S 
Sbjct: 426 FKGVATVTTTDPSRGTCVFPGSAKSNQTLG-NNTSALAPSANSTTSGCIPKYYHHPHASF 485

BLAST of Lcy03g008340 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 456.1 bits (1172), Expect = 5.2e-127
Identity = 225/435 (51.72%), Postives = 308/435 (70.80%), Query Frame = 0

Query: 22  GAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKALANSSIEVIVGVTNE 81
           G+++GVN+GTD+S++P  + +VA+LK+ +I H+RLYNAD  LL ALAN+ I+VI+ + N+
Sbjct: 29  GSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPND 88

Query: 82  EVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKAL 141
           ++L IG+S + AA WV +NV A+ P T ITA++VGSEVLT++ +  PVLV A+ ++H AL
Sbjct: 89  QLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAAL 148

Query: 142 VAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFLKNTKSYYMLNAYPY 201
           ++ANL+ LIKVSTP S  +I  PFPPS A FN S N+ I  LL FL++T SY M+N YPY
Sbjct: 149 LSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPY 208

Query: 202 YGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPV 261
             Y   NG+ PLDYALF+ +P  K+ VD NTL  Y++ FDAMVDATY+++   NF+ IPV
Sbjct: 209 IDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPV 268

Query: 262 VVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTIPINTYLYELFNEDK 321
           +VTE+GWPS G  NEPDAT+ NA TY +NLIR V N +G P +P I ++TY+YEL+NED 
Sbjct: 269 LVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDT 328

Query: 322 RPGPVSEKNWGILFPNGSTVYPLGLA-SGRATANSSV--VYCVAKEGADEDKLQDGLNWA 381
           + G +SEKNWG+   NG  VY L L  SG   AN +    YC A+EGAD   LQ  L+WA
Sbjct: 329 KAG-LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWA 388

Query: 382 CGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDFDGTAMLTTNDPSH 441
           CG G  +C  I+QG  C+ P+N+  HA+YA++ YY +  +    C+F+G A +TT DPSH
Sbjct: 389 CGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPSH 448

Query: 442 GSCIYTGSSNSSSGG 454
           G+C++ GS  +   G
Sbjct: 449 GTCVFAGSRGNGRNG 462

BLAST of Lcy03g008340 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 438.7 bits (1127), Expect = 8.6e-122
Identity = 231/471 (49.04%), Postives = 308/471 (65.39%), Query Frame = 0

Query: 24  FVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKALANSSIEVIVGVTNEEV 83
           FVG N+GTDVSNL S +++V  L++ ++ H+RLY+AD +LLKALA + + VI+ V N ++
Sbjct: 43  FVGFNIGTDVSNLLSPTELVKFLQAQKVNHVRLYDADPELLKALAKTKVRVIISVPNNQL 102

Query: 84  LRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKALVA 143
           L IG S + AA+W+ +NV AY P T ITAI+VG EVLTT+P   P+L+PA+ SL+ ALVA
Sbjct: 103 LAIGSSNSTAASWIGRNVVAYYPETLITAISVGDEVLTTVPSSAPLLLPAIESLYNALVA 162

Query: 144 ANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFLKNTKSYYMLNAYPYYG 203
           +NL+  IKVSTP +  I+   FPPS A FN +W+S +  LLQFL  T S  M+N YPYY 
Sbjct: 163 SNLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYV 222

Query: 204 YTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVV 263
           Y    G+ PLD  LF  L   K++VDPNTL HY ++ DAMVDA Y S+   N S + V+V
Sbjct: 223 YMQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLV 282

Query: 264 TETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTIPINTYLYELFNEDKRP 323
           TE+GWPS G + EP ATI NA TY +NLI+ V + +G P  P +  + Y+YELFNED R 
Sbjct: 283 TESGWPSKGDSKEPYATIDNADTYNSNLIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRA 342

Query: 324 GPVSEKNWGILFPNGSTVYPLGLA-SGRATANSSV--VYCVAKEGADEDKLQDGLNWACG 383
            PVSE +WG+ + N + VY L ++ SG   AN +    YC+A +G D   LQ  L+WACG
Sbjct: 343 PPVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACG 402

Query: 384 QGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDFDGTAMLTTNDPSHGS 443
            G +NC  IQ G  C+ PNN+  HAS+A+N YYQK   A G+CDF G AM+TT DPSHGS
Sbjct: 403 PGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGS 462

Query: 444 CIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVS---SFQLLILVIFS 489
           CI+ GS       G    T +  + +  G ++   +S      ++ILV FS
Sbjct: 463 CIFPGSKKV----GNRTQTVVNSTEVAAGEATSRSLSRGFCVTIMILVTFS 509

BLAST of Lcy03g008340 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.1e-88
Identity = 191/499 (38.28%), Postives = 283/499 (56.71%), Query Frame = 0

Query: 9   VILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKALA 68
           ++LLL   C    G  VGV  G    +LP+ S +V +++ H I ++R+Y+ +SQ+LKA  
Sbjct: 13  ILLLLLDCC---YGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFG 72

Query: 69  NSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHV-- 128
           N+SIE+++GV N ++    +S +    W+  +V  Y P T IT I VG+E  T  PH+  
Sbjct: 73  NTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE-STDDPHINA 132

Query: 129 GPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQF 188
              +VPAM ++  AL    L+  IKVST  S+ I+ R FPPS  AFN+S+   +  +L+F
Sbjct: 133 SSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEF 192

Query: 189 LKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDA 248
           L   KS +M++ YPYY Y        LDY LF S     +++DPNT   Y +MFDA VDA
Sbjct: 193 LAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFES---SSEVIDPNTGLLYKNMFDAQVDA 252

Query: 249 TYYSIDAFNFSGIPVVVTETGWPSFGGANE-PDATIQNAGTYINNLIRRVSNDSGPPSQP 308
            YY++ A NF  I ++VTETGWP+ G   E   A+  NA TY +N+IR V  + G P++P
Sbjct: 253 LYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKP 312

Query: 309 TIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGL----------ASGRATANS 368
              +N Y++ LFNE+++ G  SE+NWG+ +P+ ++VY L            +SG  ++ S
Sbjct: 313 GEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGS 372

Query: 369 SVVYCVAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQK 428
           S  +C+A   A E  L+  L+WACG G  +C AIQ  +PCF P+ + +HAS+ +N Y+Q+
Sbjct: 373 SNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQ 432

Query: 429 MHSAGGTCDFDGTAMLTTNDPSHGSCIY-TGSSNSSSGGGFSPPTALGPSGLLPGSSSKL 488
             +    C F G  +    DPS+  CIY T   N +     +  TAL  S   P  +  L
Sbjct: 433 NRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKA---TNATALTSSASTPRGNELL 492

Query: 489 Q-VSSFQLLILVIFSWALM 493
           Q +    L+I + FS   M
Sbjct: 493 QWILKLCLMISLFFSLQTM 501

BLAST of Lcy03g008340 vs. ExPASy TrEMBL
Match: A0A6J1KUY8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498933 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.2e-265
Identity = 462/496 (93.15%), Postives = 476/496 (95.97%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP VGPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWNST+
Sbjct: 121 STIPQVGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNSTM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPG VSEKNWGILF NGSTVYPL LASGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGSVSEKNWGILFSNGSTVYPLDLASGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+ G
Sbjct: 361 VAKHGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHAGG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSSSGGG SPPTALGPS  LPG+SSKLQVSS+Q
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSSGGGVSPPTALGPSSQLPGASSKLQVSSYQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 496

BLAST of Lcy03g008340 vs. ExPASy TrEMBL
Match: A0A6J1ENA7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434126 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.6e-265
Identity = 462/496 (93.15%), Postives = 478/496 (96.37%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP  GPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWN+T+
Sbjct: 121 STIPQAGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPGPVSEKNWGILF NGSTVYPLGLASGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGPVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSS GGG SPP ALGPSG LPG+SSKLQVSS+Q
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSGGGGVSPP-ALGPSGQLPGASSKLQVSSYQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 495

BLAST of Lcy03g008340 vs. ExPASy TrEMBL
Match: A0A1S3BNZ4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491954 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 4.7e-264
Identity = 461/496 (92.94%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRERC GIV+LLLFGMC+NALGAFVGVNLGT VSNLPS SDIVAILKSHQITHLRLYNAD
Sbjct: 1   MRERCCGIVVLLLFGMCINALGAFVGVNLGTSVSNLPSPSDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
            QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAA+LPGTNITAIAVGSEVL
Sbjct: 61  PQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAHLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTA+F+ASWNSTI
Sbjct: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTASFDASWNSTI 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPYYGYTSGNG FPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGSFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSI+AFNFS IPVVVTETGWPSFGGANEPDATIQNAGTY++NLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIEAFNFSEIPVVVTETGWPSFGGANEPDATIQNAGTYVSNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTYLYELFNEDKRPGP+SEKNWGILFPNGS VYPL L SGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYLYELFNEDKRPGPISEKNWGILFPNGSAVYPLSLMSGRATANSSEVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKM  AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMRGAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCI+TGSSNSS GGGFSPP ALGPSG LPG+SSKLQ+SSFQ
Sbjct: 421 GTCDFDGTATLTTVDPSHGSCIFTGSSNSSGGGGFSPP-ALGPSGQLPGASSKLQMSSFQ 480

Query: 481 LLILVIFSWALMFTLT 497
           L ILVIFSWALMF  T
Sbjct: 481 LFILVIFSWALMFIFT 495

BLAST of Lcy03g008340 vs. ExPASy TrEMBL
Match: A0A6J1DG93 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020775 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.4e-262
Identity = 450/491 (91.65%), Postives = 468/491 (95.32%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHG VILLLFGMC+NALGAFVGVNLGTDVS LPSASD VAILKSHQITHLRLYNAD
Sbjct: 1   MRESCHGTVILLLFGMCINALGAFVGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           T IPHVGPVLVPAMYS+HKALVAANLN +IKVSTPQSMDIIPR FPPSTA FNASWNSTI
Sbjct: 121 TAIPHVGPVLVPAMYSIHKALVAANLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTI 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFL NTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLNNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP FGG NEPDATIQNAGTY NNLIRRV+NDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWFGGGNEPDATIQNAGTYCNNLIRRVTNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTYLYELFNEDK+PGPVSEKNWGILFPNGS VYPLG+ SGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYLYELFNEDKKPGPVSEKNWGILFPNGSAVYPLGVTSGRATANSSAVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANCVAIQ GRPC+LPNN+TNHASYA+NDYYQKMHSAG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTAM+TT DPSHGSCIY+GSSNSSSGGGFSPPTA+GPSG  PG+SSKL++SSFQ
Sbjct: 421 GTCDFDGTAMITTIDPSHGSCIYSGSSNSSSGGGFSPPTAMGPSGQFPGASSKLKISSFQ 480

Query: 481 LLILVIFSWAL 492
            +ILV FSWAL
Sbjct: 481 FMILVSFSWAL 491

BLAST of Lcy03g008340 vs. ExPASy TrEMBL
Match: A0A0A0LXK4 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G630300 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.7e-262
Identity = 458/496 (92.34%), Postives = 473/496 (95.36%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRER  GIVILLLFGMC+NALGAFVGVNLGT VSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MRERWRGIVILLLFGMCINALGAFVGVNLGTGVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
            QLLKAL NSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAA+LPGTNITAIAVGSEVL
Sbjct: 61  FQLLKALTNSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAHLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPR FPPSTA+F+ASWNSTI
Sbjct: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRAFPPSTASFDASWNSTI 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKS+YMLNAYPYYGYTSGNG FPLDYALFRSLP IKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSFYMLNAYPYYGYTSGNGSFPLDYALFRSLPTIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DA+VDATYYSI+AFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYI+NLIRRVSNDSG
Sbjct: 241 DALVDATYYSIEAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYISNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPGP+SEKNWGILFPNGS VYPL   SGRATANSSVVYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGPISEKNWGILFPNGSAVYPLSSMSGRATANSSVVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKM  AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMRGAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFD TAMLTT DPSHGSCI+TGSSNSS GGGFSPP A GPSGLLPG+SSKLQ+SSFQ
Sbjct: 421 GTCDFDSTAMLTTVDPSHGSCIFTGSSNSSGGGGFSPP-ASGPSGLLPGASSKLQISSFQ 480

Query: 481 LLILVIFSWALMFTLT 497
           L ILVIFSWALMF  T
Sbjct: 481 LFILVIFSWALMFIFT 495

BLAST of Lcy03g008340 vs. NCBI nr
Match: XP_038879038.1 (glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879039.1 glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879040.1 glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida])

HSP 1 Score: 943.0 bits (2436), Expect = 1.1e-270
Identity = 474/496 (95.56%), Postives = 482/496 (97.18%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRER HGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MRERGHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           SQ+LKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  SQMLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTA+F ASWNSTI
Sbjct: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTASFEASWNSTI 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPYYGY SGNG FPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYYGYNSGNGSFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYI+NLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYISNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTYLYELFNEDK+PGP+SEKNWGILFPNGS VYPLGL SGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYLYELFNEDKKPGPISEKNWGILFPNGSAVYPLGLMSGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNITNHASYAYNDYYQKM  AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITNHASYAYNDYYQKMRGAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTAMLTT DPSHGSCI+TGSSN SSGGGFSPPTA GPSGLLPG+SSKLQVSSFQ
Sbjct: 421 GTCDFDGTAMLTTVDPSHGSCIFTGSSN-SSGGGFSPPTASGPSGLLPGASSKLQVSSFQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLILVIFSWALMF LT
Sbjct: 481 LLILVIFSWALMFILT 495

BLAST of Lcy03g008340 vs. NCBI nr
Match: XP_023006087.1 (glucan endo-1,3-beta-glucosidase 4-like [Cucurbita maxima] >XP_023006096.1 glucan endo-1,3-beta-glucosidase 4-like [Cucurbita maxima])

HSP 1 Score: 923.3 bits (2385), Expect = 8.8e-265
Identity = 462/496 (93.15%), Postives = 476/496 (95.97%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP VGPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWNST+
Sbjct: 121 STIPQVGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNSTM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPG VSEKNWGILF NGSTVYPL LASGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGSVSEKNWGILFSNGSTVYPLDLASGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+ G
Sbjct: 361 VAKHGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHAGG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSSSGGG SPPTALGPS  LPG+SSKLQVSS+Q
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSSGGGVSPPTALGPSSQLPGASSKLQVSSYQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 496

BLAST of Lcy03g008340 vs. NCBI nr
Match: KAG7022153.1 (Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 462/496 (93.15%), Postives = 479/496 (96.57%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP VGPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWN+T+
Sbjct: 121 STIPQVGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPGPVSEKNWGILF NGSTVYPLGLASGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGPVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSS GGG SPP ALGPSG LPG+SSKLQVSS++
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSGGGGVSPP-ALGPSGQLPGASSKLQVSSYK 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 495

BLAST of Lcy03g008340 vs. NCBI nr
Match: XP_022927211.1 (glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata] >XP_022927220.1 glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 462/496 (93.15%), Postives = 478/496 (96.37%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP  GPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWN+T+
Sbjct: 121 STIPQAGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPGPVSEKNWGILF NGSTVYPLGLASGRATANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGPVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSS GGG SPP ALGPSG LPG+SSKLQVSS+Q
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSGGGGVSPP-ALGPSGQLPGASSKLQVSSYQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 495

BLAST of Lcy03g008340 vs. NCBI nr
Match: KAG6588238.1 (Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 462/496 (93.15%), Postives = 478/496 (96.37%), Query Frame = 0

Query: 1   MRERCHGIVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60
           MRE CHGIVILLLFGMCVN+LGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD
Sbjct: 1   MREGCHGIVILLLFGMCVNSLGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNAD 60

Query: 61  SQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120
           +QLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL
Sbjct: 61  AQLLKALANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVL 120

Query: 121 TTIPHVGPVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTI 180
           +TIP VGPVLVPA+YSLHKALVAANLNYL+KVSTPQSMD+IPRPFPPSTA FNASWN+T+
Sbjct: 121 STIPQVGPVLVPALYSLHKALVAANLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATM 180

Query: 181 YQLLQFLKNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240
           YQLLQFLKNTKSYYMLNAYPY GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF
Sbjct: 181 YQLLQFLKNTKSYYMLNAYPYLGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMF 240

Query: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSG 300
           DAMVDATYYSIDAFNFSGIPVVVTETGWP  GGANEPDATIQNAGTY+NNLIRRVSNDSG
Sbjct: 241 DAMVDATYYSIDAFNFSGIPVVVTETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSG 300

Query: 301 PPSQPTIPINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATANSSVVYC 360
           PPSQPTIPINTY+YELFNEDKRPGPVSEKNWGILF NGSTVYPLGLASGRA ANSS VYC
Sbjct: 301 PPSQPTIPINTYIYELFNEDKRPGPVSEKNWGILFSNGSTVYPLGLASGRAIANSSTVYC 360

Query: 361 VAKEGADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAG 420
           VAK+GADEDKLQDGLNWACGQGGANC AIQQGRPCFLPNNIT+HASYAYNDYYQKMH+AG
Sbjct: 361 VAKDGADEDKLQDGLNWACGQGGANCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAG 420

Query: 421 GTCDFDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQ 480
           GTCDFDGTA LTT DPSHGSCIYTGSSNSS GGG SPP ALGPSG LPG+SSKLQVSS+Q
Sbjct: 421 GTCDFDGTATLTTIDPSHGSCIYTGSSNSSGGGGVSPP-ALGPSGQLPGASSKLQVSSYQ 480

Query: 481 LLILVIFSWALMFTLT 497
           LLI VIFSWALM +LT
Sbjct: 481 LLIWVIFSWALMVSLT 495

BLAST of Lcy03g008340 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-192
Identity = 327/487 (67.15%), Postives = 405/487 (83.16%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           +++LL    C NA  AF+GVN+GTD++N+P  SDIV +LKS QITH+RLY+A+S +LKA 
Sbjct: 11  LLLLLSILACSNA--AFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAF 70

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           AN+SIEV+VGVTNEE+L+IG  P+AAAAWVNKNVAAY+P TNITAIAVGSEVLTTIPHV 
Sbjct: 71  ANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVA 130

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
           P+L  A+ ++HKALVA+NLN+ +KVS+P SMDI+P+PFPPST+ F+ SWN+T+YQLLQFL
Sbjct: 131 PILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFL 190

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           KNT S++MLNAYPYYGYT+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA 
Sbjct: 191 KNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAA 250

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           YYS++A NFS IPVVVTETGWPS GG++E  AT+ NA T+  NLI+RV N+SGPPSQP I
Sbjct: 251 YYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDI 310

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATA--NSSVVYCVAKEG 367
           PINTY+YEL+NEDKR GPVSE+NWGILFPNG++VYPL L+ G ++A  N S ++CVAK  
Sbjct: 311 PINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKAD 370

Query: 368 ADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDF 427
           AD+DKL DGLNWACGQG ANC AIQ G+PC+LPN++ +HAS+A+NDYYQKM SAGGTCDF
Sbjct: 371 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDF 430

Query: 428 DGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQLLILV 487
           DGTA+ TT DPS+ +C YTGS N+++  G  PP ALGP+  L G+++   + S+ L IL 
Sbjct: 431 DGTAITTTRDPSYRTCAYTGSLNANATNGNFPPDALGPASPLGGNANARIIFSYHLPILA 490

Query: 488 IFSWALM 493
             +  L+
Sbjct: 491 PLALTLL 495

BLAST of Lcy03g008340 vs. TAIR 10
Match: AT3G13560.3 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-192
Identity = 327/487 (67.15%), Postives = 405/487 (83.16%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           +++LL    C NA  AF+GVN+GTD++N+P  SDIV +LKS QITH+RLY+A+S +LKA 
Sbjct: 11  LLLLLSILACSNA--AFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAF 70

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           AN+SIEV+VGVTNEE+L+IG  P+AAAAWVNKNVAAY+P TNITAIAVGSEVLTTIPHV 
Sbjct: 71  ANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVA 130

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
           P+L  A+ ++HKALVA+NLN+ +KVS+P SMDI+P+PFPPST+ F+ SWN+T+YQLLQFL
Sbjct: 131 PILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFL 190

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           KNT S++MLNAYPYYGYT+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA 
Sbjct: 191 KNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAA 250

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           YYS++A NFS IPVVVTETGWPS GG++E  AT+ NA T+  NLI+RV N+SGPPSQP I
Sbjct: 251 YYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDI 310

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATA--NSSVVYCVAKEG 367
           PINTY+YEL+NEDKR GPVSE+NWGILFPNG++VYPL L+ G ++A  N S ++CVAK  
Sbjct: 311 PINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKAD 370

Query: 368 ADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDF 427
           AD+DKL DGLNWACGQG ANC AIQ G+PC+LPN++ +HAS+A+NDYYQKM SAGGTCDF
Sbjct: 371 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDF 430

Query: 428 DGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQLLILV 487
           DGTA+ TT DPS+ +C YTGS N+++  G  PP ALGP+  L G+++   + S+ L IL 
Sbjct: 431 DGTAITTTRDPSYRTCAYTGSLNANATNGNFPPDALGPASPLGGNANARIIFSYHLPILA 490

Query: 488 IFSWALM 493
             +  L+
Sbjct: 491 PLALTLL 495

BLAST of Lcy03g008340 vs. TAIR 10
Match: AT3G13560.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-192
Identity = 327/487 (67.15%), Postives = 405/487 (83.16%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           +++LL    C NA  AF+GVN+GTD++N+P  SDIV +LKS QITH+RLY+A+S +LKA 
Sbjct: 11  LLLLLSILACSNA--AFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAF 70

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           AN+SIEV+VGVTNEE+L+IG  P+AAAAWVNKNVAAY+P TNITAIAVGSEVLTTIPHV 
Sbjct: 71  ANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVA 130

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
           P+L  A+ ++HKALVA+NLN+ +KVS+P SMDI+P+PFPPST+ F+ SWN+T+YQLLQFL
Sbjct: 131 PILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFL 190

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           KNT S++MLNAYPYYGYT+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA 
Sbjct: 191 KNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAA 250

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           YYS++A NFS IPVVVTETGWPS GG++E  AT+ NA T+  NLI+RV N+SGPPSQP I
Sbjct: 251 YYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDI 310

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLASGRATA--NSSVVYCVAKEG 367
           PINTY+YEL+NEDKR GPVSE+NWGILFPNG++VYPL L+ G ++A  N S ++CVAK  
Sbjct: 311 PINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKAD 370

Query: 368 ADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDF 427
           AD+DKL DGLNWACGQG ANC AIQ G+PC+LPN++ +HAS+A+NDYYQKM SAGGTCDF
Sbjct: 371 ADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDF 430

Query: 428 DGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGPSGLLPGSSSKLQVSSFQLLILV 487
           DGTA+ TT DPS+ +C YTGS N+++  G  PP ALGP+  L G+++   + S+ L IL 
Sbjct: 431 DGTAITTTRDPSYRTCAYTGSLNANATNGNFPPDALGPASPLGGNANARIIFSYHLPILA 490

Query: 488 IFSWALM 493
             +  L+
Sbjct: 491 PLALTLL 495

BLAST of Lcy03g008340 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 459.1 bits (1180), Expect = 4.4e-129
Identity = 240/496 (48.39%), Postives = 330/496 (66.53%), Query Frame = 0

Query: 8   IVILLLFGMCVNALGAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKAL 67
           ++ L LF     +  + +GVN+GT+V+N+PS + +VA+LKS  I  +RLY+AD  +L A 
Sbjct: 6   LLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAF 65

Query: 68  ANSSIEVIVGVTNEEVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVG 127
           A++ ++VI+ V N+++L I +S A AA WV +NVAAY P TNIT IAVGSEVLT++ +  
Sbjct: 66  AHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAA 125

Query: 128 PVLVPAMYSLHKALVAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFL 187
            VLV A+  +  ALV ANL+  IKVSTP S  II   FPPS A FN +W+  I  LL+FL
Sbjct: 126 SVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFL 185

Query: 188 KNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDAT 247
           ++T S  +LN YPY+ Y   NG+ PLDYALF+ L A K+ VD NTL HY ++FDA+VDA 
Sbjct: 186 QSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAA 245

Query: 248 YYSIDAFNFSGIPVVVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTI 307
           Y+++   NF+ IP+VVTE+GWPS GG +E DAT++NA TY +NLI+ V N +G P  P  
Sbjct: 246 YFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGT 305

Query: 308 PINTYLYELFNEDKRPGPVSEKNWGILFPNGSTVYPLGLA-SGRATANSSV--VYCVAKE 367
            + TY+YEL+NED RPGPVSEKNWG+ + NG+ VY L LA +G   AN +    +C+AKE
Sbjct: 306 AVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKE 365

Query: 368 GADEDKLQDGLNWACGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCD 427
             D   LQ  L+WACG G  +C A+ QG  C+ P+++  H++YA+N YYQKM  A G+CD
Sbjct: 366 KVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCD 425

Query: 428 FDGTAMLTTNDPSHGSCIYTGSSNSSSGGGFSPPTALGP------SGLLP-------GSS 487
           F G A +TT DPS G+C++ GS+ S+   G +  +AL P      SG +P        S 
Sbjct: 426 FKGVATVTTTDPSRGTCVFPGSAKSNQTLG-NNTSALAPSANSTTSGCIPKYYHHPHASF 485

BLAST of Lcy03g008340 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 456.1 bits (1172), Expect = 3.7e-128
Identity = 225/435 (51.72%), Postives = 308/435 (70.80%), Query Frame = 0

Query: 22  GAFVGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQLLKALANSSIEVIVGVTNE 81
           G+++GVN+GTD+S++P  + +VA+LK+ +I H+RLYNAD  LL ALAN+ I+VI+ + N+
Sbjct: 29  GSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPND 88

Query: 82  EVLRIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKAL 141
           ++L IG+S + AA WV +NV A+ P T ITA++VGSEVLT++ +  PVLV A+ ++H AL
Sbjct: 89  QLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAAL 148

Query: 142 VAANLNYLIKVSTPQSMDIIPRPFPPSTAAFNASWNSTIYQLLQFLKNTKSYYMLNAYPY 201
           ++ANL+ LIKVSTP S  +I  PFPPS A FN S N+ I  LL FL++T SY M+N YPY
Sbjct: 149 LSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPY 208

Query: 202 YGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPV 261
             Y   NG+ PLDYALF+ +P  K+ VD NTL  Y++ FDAMVDATY+++   NF+ IPV
Sbjct: 209 IDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPV 268

Query: 262 VVTETGWPSFGGANEPDATIQNAGTYINNLIRRVSNDSGPPSQPTIPINTYLYELFNEDK 321
           +VTE+GWPS G  NEPDAT+ NA TY +NLIR V N +G P +P I ++TY+YEL+NED 
Sbjct: 269 LVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDT 328

Query: 322 RPGPVSEKNWGILFPNGSTVYPLGLA-SGRATANSSV--VYCVAKEGADEDKLQDGLNWA 381
           + G +SEKNWG+   NG  VY L L  SG   AN +    YC A+EGAD   LQ  L+WA
Sbjct: 329 KAG-LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWA 388

Query: 382 CGQGGANCVAIQQGRPCFLPNNITNHASYAYNDYYQKMHSAGGTCDFDGTAMLTTNDPSH 441
           CG G  +C  I+QG  C+ P+N+  HA+YA++ YY +  +    C+F+G A +TT DPSH
Sbjct: 389 CGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPSH 448

Query: 442 GSCIYTGSSNSSSGG 454
           G+C++ GS  +   G
Sbjct: 449 GTCVFAGSRGNGRNG 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CD82.3e-19167.15Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ... [more]
Q9ZU916.1e-12848.39Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q9C7U55.2e-12751.72Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
O653998.6e-12249.04Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9FJU92.1e-8838.28Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
A0A6J1KUY84.2e-26593.15(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498933 PE=... [more]
A0A6J1ENA75.6e-26593.15(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434126 P... [more]
A0A1S3BNZ44.7e-26492.94(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491954 PE=3 SV... [more]
A0A6J1DG933.4e-26291.65(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020775 ... [more]
A0A0A0LXK45.7e-26292.34(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G630300 PE=3... [more]
Match NameE-valueIdentityDescription
XP_038879038.11.1e-27095.56glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879039.1 gluc... [more]
XP_023006087.18.8e-26593.15glucan endo-1,3-beta-glucosidase 4-like [Cucurbita maxima] >XP_023006096.1 gluca... [more]
KAG7022153.11.1e-26493.15Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022927211.11.1e-26493.15glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata] >XP_022927220.1 glu... [more]
KAG6588238.12.0e-26493.15Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT3G13560.11.6e-19267.15O-Glycosyl hydrolases family 17 protein [more]
AT3G13560.31.6e-19267.15O-Glycosyl hydrolases family 17 protein [more]
AT3G13560.21.6e-19267.15O-Glycosyl hydrolases family 17 protein [more]
AT2G01630.14.4e-12948.39O-Glycosyl hydrolases family 17 protein [more]
AT1G66250.13.7e-12851.72O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 358..443
e-value: 1.7E-47
score: 173.8
IPR012946X8 domainPFAMPF07983X8coord: 359..430
e-value: 3.5E-19
score: 69.3
NoneNo IPR availableGENE3D1.20.58.1040coord: 353..451
e-value: 1.7E-9
score: 39.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..352
e-value: 9.0E-109
score: 365.3
NoneNo IPR availablePANTHERPTHR32227:SF274GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 4coord: 3..452
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 25..344
e-value: 2.6E-77
score: 260.5
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 259..272
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 3..452
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 25..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g008340.1Lcy03g008340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds