Lcy03g008300 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g008300
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAmino acid/polyamine transporter
LocationChr03: 40317289 .. 40319006 (+)
RNA-Seq ExpressionLcy03g008300
SyntenyLcy03g008300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTTTGTTAGCATCGGCCAATCAATCATGGCTGACACTAAACAATCCCTCAACAAGTCCAACCACAATCAACCCACTCCAGATTCTTCTTCCATTTCTCAAACCCTCCCTACCACCACCGCCACTTCCGCCATCGCCGCCAGAAGGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTACGGTGAAGAGCCCGCCGTCCAGGCCGCCGGACCCCTCCTCGCCATCGTCGGATTCATCGTCTTCCCTTTCATATGGAGCGTTCCCGAGGCGCTCATCACGGCGGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGATTCGTCATCTGGGCCGAGAGAGCCTTCGGTCCCTTCTGGGGTTCTCTCATGGGCACCTGGAAACTCCTCAGCGGCGTCATCAACATCGCCGCCTTCCCAGTCCTCTGCATCGATTACATCAAGAGAATCGCTCCGCCGCTCGAATCCGGCTGGCCCCGCCGCATCGCCGTCCTCGCCTCCACCCTCGTCCTCGCCCTCCTCAACTACATCGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTGGCCTTCTTGTCGCTCTTGCCCTTCATCTTAATGACCTTCATCGCCATCCCTAAAATCCACCCCCATCGATGGCTCAGTTTAGGCGATAAAGGGGTCAAGAAAGATTGGAATCTCTACCTCAACACTCTGTTTTGGAACCTCAATTTCTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCGAAAACCCCCAAAAAACATTCCCCATGGCTCTGTTCGTATCCGTGATTTTCACTTGTCTCTCTTATCTAATCCCACTTCTGGCCGTCATCGGCGCCGTCGATGTAGAGCAATCCGCTTGGGGTTCTGGCTTCCACGCCCAAGCCGCCGGAATCATCGCCGGAAAATGGCTGAAAATCCTCCTCGAAATCGGGTCGTCCTTGTCGGCGATTGGGCTATTCGAGGCCCAACTGAGTAGCAGCGCGTATCAGATATTGGGTATGGCGGAAATCGGGATTCTGCCCAAATTCTTCGCGGCCAGAGCGAAATGGTTCAACACCCCATGGATTGGGATTGTGATCTGCACTGCAATTTCTCTCGCAGTTTCGTATATGAACTTCGCCGACATCGTTGCATCGGCTAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGCATGAAAAGGCCGTTCAAGGTTCCATTGAAGCTGCCGGGTCTGATCGTGATGTGTTTGGTTCCGTCGGCGTTTTTGGTGGTGGTGATGGTTTTTACTCACAAGACTGTGTTCTTGGTGAGCGCTGTAATGACTGCGGCTGGGATTATCTGGTTTGTGTTGATGAAGATTTGTAGGAAGAAGAAGATATTCGAGTTCAATCCTCGGCTTGAAGCCATTGAAGAATTGTGAGATTTGACTCTGCTTTTGATTATTATATGCAGTTTGCTTAAGATTGTGTTTGGTTAAATTCTACTTTTGATTTTGTAGATAATAGTTGGTAGTCTACTTACTTGTTTGAAGGAATTATATTTTAGGATTTTGTGATCGTTTCGATTCTCTACTCTCCTTTATAATGTAATTGTTCATACGATTTTATCAGTTCTCTGGAAATTAGGGATCGGTAGAGATAACATTCTTGTTTTTATAGGTTAAATTATAAA

mRNA sequence

CTGTTTGTTAGCATCGGCCAATCAATCATGGCTGACACTAAACAATCCCTCAACAAGTCCAACCACAATCAACCCACTCCAGATTCTTCTTCCATTTCTCAAACCCTCCCTACCACCACCGCCACTTCCGCCATCGCCGCCAGAAGGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTACGGTGAAGAGCCCGCCGTCCAGGCCGCCGGACCCCTCCTCGCCATCGTCGGATTCATCGTCTTCCCTTTCATATGGAGCGTTCCCGAGGCGCTCATCACGGCGGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGATTCGTCATCTGGGCCGAGAGAGCCTTCGGTCCCTTCTGGGGTTCTCTCATGGGCACCTGGAAACTCCTCAGCGGCGTCATCAACATCGCCGCCTTCCCAGTCCTCTGCATCGATTACATCAAGAGAATCGCTCCGCCGCTCGAATCCGGCTGGCCCCGCCGCATCGCCGTCCTCGCCTCCACCCTCGTCCTCGCCCTCCTCAACTACATCGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTGGCCTTCTTGTCGCTCTTGCCCTTCATCTTAATGACCTTCATCGCCATCCCTAAAATCCACCCCCATCGATGGCTCAGTTTAGGCGATAAAGGGGTCAAGAAAGATTGGAATCTCTACCTCAACACTCTGTTTTGGAACCTCAATTTCTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCGAAAACCCCCAAAAAACATTCCCCATGGCTCTGTTCGTATCCGTGATTTTCACTTGTCTCTCTTATCTAATCCCACTTCTGGCCGTCATCGGCGCCGTCGATGTAGAGCAATCCGCTTGGGGTTCTGGCTTCCACGCCCAAGCCGCCGGAATCATCGCCGGAAAATGGCTGAAAATCCTCCTCGAAATCGGGTCGTCCTTGTCGGCGATTGGGCTATTCGAGGCCCAACTGAGTAGCAGCGCGTATCAGATATTGGGTATGGCGGAAATCGGGATTCTGCCCAAATTCTTCGCGGCCAGAGCGAAATGGTTCAACACCCCATGGATTGGGATTGTGATCTGCACTGCAATTTCTCTCGCAGTTTCGTATATGAACTTCGCCGACATCGTTGCATCGGCTAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGCATGAAAAGGCCGTTCAAGGTTCCATTGAAGCTGCCGGGTCTGATCGTGATGTGTTTGGTTCCGTCGGCGTTTTTGGTGGTGGTGATGGTTTTTACTCACAAGACTGTGTTCTTGGTGAGCGCTGTAATGACTGCGGCTGGGATTATCTGGTTTGTGTTGATGAAGATTTGTAGGAAGAAGAAGATATTCGAGTTCAATCCTCGGCTTGAAGCCATTGAAGAATTGTGAGATTTGACTCTGCTTTTGATTATTATATGCAGTTTGCTTAAGATTGTGTTTGGTTAAATTCTACTTTTGATTTTGTAGATAATAGTTGGTAGTCTACTTACTTGTTTGAAGGAATTATATTTTAGGATTTTGTGATCGTTTCGATTCTCTACTCTCCTTTATAATGTAATTGTTCATACGATTTTATCAGTTCTCTGGAAATTAGGGATCGGTAGAGATAACATTCTTGTTTTTATAGGTTAAATTATAAA

Coding sequence (CDS)

ATGGCTGACACTAAACAATCCCTCAACAAGTCCAACCACAATCAACCCACTCCAGATTCTTCTTCCATTTCTCAAACCCTCCCTACCACCACCGCCACTTCCGCCATCGCCGCCAGAAGGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTACGGTGAAGAGCCCGCCGTCCAGGCCGCCGGACCCCTCCTCGCCATCGTCGGATTCATCGTCTTCCCTTTCATATGGAGCGTTCCCGAGGCGCTCATCACGGCGGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGATTCGTCATCTGGGCCGAGAGAGCCTTCGGTCCCTTCTGGGGTTCTCTCATGGGCACCTGGAAACTCCTCAGCGGCGTCATCAACATCGCCGCCTTCCCAGTCCTCTGCATCGATTACATCAAGAGAATCGCTCCGCCGCTCGAATCCGGCTGGCCCCGCCGCATCGCCGTCCTCGCCTCCACCCTCGTCCTCGCCCTCCTCAACTACATCGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTGGCCTTCTTGTCGCTCTTGCCCTTCATCTTAATGACCTTCATCGCCATCCCTAAAATCCACCCCCATCGATGGCTCAGTTTAGGCGATAAAGGGGTCAAGAAAGATTGGAATCTCTACCTCAACACTCTGTTTTGGAACCTCAATTTCTGGGATAACGTCAGCACTCTCGCCGGAGAAGTCGAAAACCCCCAAAAAACATTCCCCATGGCTCTGTTCGTATCCGTGATTTTCACTTGTCTCTCTTATCTAATCCCACTTCTGGCCGTCATCGGCGCCGTCGATGTAGAGCAATCCGCTTGGGGTTCTGGCTTCCACGCCCAAGCCGCCGGAATCATCGCCGGAAAATGGCTGAAAATCCTCCTCGAAATCGGGTCGTCCTTGTCGGCGATTGGGCTATTCGAGGCCCAACTGAGTAGCAGCGCGTATCAGATATTGGGTATGGCGGAAATCGGGATTCTGCCCAAATTCTTCGCGGCCAGAGCGAAATGGTTCAACACCCCATGGATTGGGATTGTGATCTGCACTGCAATTTCTCTCGCAGTTTCGTATATGAACTTCGCCGACATCGTTGCATCGGCTAATTTCATATACAGCTTGGGAATGCTGCTGGAGTTTTCGTCGTTCGTTTGGTTGAGATGGAAGCATCCGGGCATGAAAAGGCCGTTCAAGGTTCCATTGAAGCTGCCGGGTCTGATCGTGATGTGTTTGGTTCCGTCGGCGTTTTTGGTGGTGGTGATGGTTTTTACTCACAAGACTGTGTTCTTGGTGAGCGCTGTAATGACTGCGGCTGGGATTATCTGGTTTGTGTTGATGAAGATTTGTAGGAAGAAGAAGATATTCGAGTTCAATCCTCGGCTTGAAGCCATTGAAGAATTGTGA

Protein sequence

MADTKQSLNKSNHNQPTPDSSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEEL
Homology
BLAST of Lcy03g008300 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.1e-181
Identity = 317/463 (68.47%), Postives = 385/463 (83.15%), Query Frame = 0

Query: 19  DSSSISQTLPTTTATSA--IAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIV 78
           ++S  S  LP TTA S+   A  +KLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLAI+
Sbjct: 5   EASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAIL 64

Query: 79  GFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGVINIAA 138
           GF++FPFIWS+PEALITAELSTAFPGNGGFVIWA RAFG F GS+MG+ K LSGVIN+A+
Sbjct: 65  GFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVAS 124

Query: 139 FPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLSLLPFI 198
           FPVLC+ Y+ ++ P LESGWPR + + AST+VL+ LNY GL IVGY AVVL  +SL PF+
Sbjct: 125 FPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFL 184

Query: 199 LMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPM 258
           +M+ +AIPKI PHRW SLG K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKTFP+
Sbjct: 185 VMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPL 244

Query: 259 ALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSSLSA 318
           AL ++VIFTC++YLIPL AV GAV V+QS W +GFHA+AA +IAGKWLKI +EIG+ LS+
Sbjct: 245 ALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSS 304

Query: 319 IGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYMNFADI 378
           IGLFEAQLSSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +SL +SYMNF DI
Sbjct: 305 IGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDI 364

Query: 379 VASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVVVMVFT 438
           ++SANF+Y+LGM LEF+SF+WLR K P +KRP++VPLK+PGL+VMCL+PSAFLV+++VF 
Sbjct: 365 ISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFA 424

Query: 439 HKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEEL 480
            K V+L+  VMT   I W+ L+   RK KIFEFN   E I++L
Sbjct: 425 TKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFN---EVIDDL 462

BLAST of Lcy03g008300 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.3e-116
Identity = 218/470 (46.38%), Postives = 316/470 (67.23%), Query Frame = 0

Query: 2   ADTKQSLNKSNHNQPTPD-SSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPY 61
           A  K  ++  N   P P  S  ++   P T+    +   +K+T++PL+FLI++EV+GGP+
Sbjct: 12  ASQKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPF 71

Query: 62  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 121
           G E +V+AAGPLLAIVGFIVFPFIWS+PEALITAE+ T FP NGG+V+W   A GP+WG 
Sbjct: 72  GIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGF 131

Query: 122 LMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIV 181
             G  K LSGVI+ A +P+L +DY+K   P L SG PR  A+L  T+ L  LNY GL+IV
Sbjct: 132 QQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIV 191

Query: 182 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNV 241
           G  AV+L   S+LPF++M+F++IPK+ P RWL +  K    +W+LYLNTLFWNLN+WD+V
Sbjct: 192 GVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSV 251

Query: 242 STLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 301
           STL GEVENP KT P ALF +++    SY+ P+L   GA+ ++Q  W  G+ A    +I 
Sbjct: 252 STLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIG 311

Query: 302 GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 361
           G WL   ++  ++ S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TPW+GI+ 
Sbjct: 312 GVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILF 371

Query: 362 CTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIV 421
             +  + +S+++F +IVA+ N +Y  GM+LEF +FV LR K+P   RPFK+P+ + G ++
Sbjct: 372 SASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVL 431

Query: 422 MCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFN 471
           MC+ P+  + V+M FT+  V LVS      G++    +K   KK   +F+
Sbjct: 432 MCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Lcy03g008300 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.4e-113
Identity = 216/459 (47.06%), Postives = 314/459 (68.41%), Query Frame = 0

Query: 14  NQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGP 73
           N   P SS  +  +P++   AT  I   RK++++PL+FLI++EV+GGP+G E +V AAGP
Sbjct: 22  NNEVPYSSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGP 81

Query: 74  LLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGV 133
           LLA++GF++FPFIWS+PEALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGV
Sbjct: 82  LLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGV 141

Query: 134 INIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLS 193
           I+ A +PVL +DY+K   P L SG PR  ++L  T++L  LNY GLTIVG+VAV++   S
Sbjct: 142 IDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFS 201

Query: 194 LLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQ 253
           +LPF +M  I+IP++ P RWL + D G   +WNLYLNTLFWNLN+WD++STLAGEVENP 
Sbjct: 202 ILPFAVMGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPN 261

Query: 254 KTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIG 313
            T P ALF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  
Sbjct: 262 HTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAA 321

Query: 314 SSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYM 373
           ++ S +G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S++
Sbjct: 322 AATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWL 381

Query: 374 NFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVV 433
           +F +IVA+ N +Y +GM+LEF +FV +R KHP   RP+K+P+   G I+MC+ P+  +  
Sbjct: 382 SFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICA 441

Query: 434 VMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFN 471
           V+  +   V  VS VM   G +   L+    +K+  +F+
Sbjct: 442 VVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 474

BLAST of Lcy03g008300 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.9e-113
Identity = 228/476 (47.90%), Postives = 313/476 (65.76%), Query Frame = 0

Query: 4   TKQSLNKSNHNQPTPDSSSISQTLPTTTATSAI-AARRKLTLIPLIFLIYFEVAGGPYGE 63
           T +S   +N   P  +  +  + LP   A   + ++ R +++IPLIFLI++EV+GGP+G 
Sbjct: 44  TVESAQPANGAAPMGECGTEYRGLPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGI 103

Query: 64  EPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLM 123
           E +V AAGPLLAI+GF+V P IWS+PEALITAEL   FP NGG+V+W   A GP+WG   
Sbjct: 104 EDSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQ 163

Query: 124 GTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGY 183
           G  K LSGVI+ A +PVL +DY+K   P L  G PR  AV+  T VL LLNY GLT+VG+
Sbjct: 164 GWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGW 223

Query: 184 VAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVST 243
           VA+ L   SLLPF +M  IA+PK+ P RWL +    V  DWNLYLNTLFWNLN+WD++ST
Sbjct: 224 VAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSIST 283

Query: 244 LAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGK 303
           LAGEV+NP KT P ALF +VIF  ++YL PLLA  GAV +++  W  G+ A  A ++ G 
Sbjct: 284 LAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGA 343

Query: 304 WLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICT 363
           WL   ++  ++LS +G+F A++SS +YQ+LGMAE G+LP FFAAR++ + TP  GI+   
Sbjct: 344 WLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSA 403

Query: 364 AISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMC 423
           +  L +S M+F +IVA+ NF+Y  GMLLEF +F+  R + P   RP++VPL   G + M 
Sbjct: 404 SGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAML 463

Query: 424 LVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEF--NPRLEAI 477
           + P+A + VV+  +   V +VS    A G++    ++   KK+   F  NP L  I
Sbjct: 464 VPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Lcy03g008300 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.9e-113
Identity = 228/476 (47.90%), Postives = 313/476 (65.76%), Query Frame = 0

Query: 4   TKQSLNKSNHNQPTPDSSSISQTLPTTTATSAI-AARRKLTLIPLIFLIYFEVAGGPYGE 63
           T +S   +N   P  +  +  + LP   A   + ++ R +++IPLIFLI++EV+GGP+G 
Sbjct: 44  TVESAQPANGAAPMGECGTEYRGLPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGI 103

Query: 64  EPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLM 123
           E +V AAGPLLAI+GF+V P IWS+PEALITAEL   FP NGG+V+W   A GP+WG   
Sbjct: 104 EDSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQ 163

Query: 124 GTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGY 183
           G  K LSGVI+ A +PVL +DY+K   P L  G PR  AV+  T VL LLNY GLT+VG+
Sbjct: 164 GWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGW 223

Query: 184 VAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVST 243
           VA+ L   SLLPF +M  IA+PK+ P RWL +    V  DWNLYLNTLFWNLN+WD++ST
Sbjct: 224 VAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSIST 283

Query: 244 LAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGK 303
           LAGEV+NP KT P ALF +VIF  ++YL PLLA  GAV +++  W  G+ A  A ++ G 
Sbjct: 284 LAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGA 343

Query: 304 WLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICT 363
           WL   ++  ++LS +G+F A++SS +YQ+LGMAE G+LP FFAAR++ + TP  GI+   
Sbjct: 344 WLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSA 403

Query: 364 AISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMC 423
           +  L +S M+F +IVA+ NF+Y  GMLLEF +F+  R + P   RP++VPL   G + M 
Sbjct: 404 SGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAML 463

Query: 424 LVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEF--NPRLEAI 477
           + P+A + VV+  +   V +VS    A G++    ++   KK+   F  NP L  I
Sbjct: 464 VPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Lcy03g008300 vs. ExPASy TrEMBL
Match: A0A6J1KV06 (probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC111498946 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.2e-244
Identity = 434/481 (90.23%), Postives = 459/481 (95.43%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTTTAT---SAIAARRKLTLIPLIFLIYFEVAG 60
           MAD KQS NKS ++QP  DSSS SQTLPTT+AT   SA A+R+KLTLIPLIFLIYFEVAG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPF 120
           GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGL 180
           WGSLMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAV +STL+LALLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240
           TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300
           DNVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAG
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 IIAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIG 360
           IIAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA RAKWFNTPWIG
Sbjct: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPWIG 360

Query: 361 IVICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPG 420
           IVICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP 
Sbjct: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420

Query: 421 LIVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIE 479
           LI+MCL+PSAFLVV+MVFTHK V +VSAVMTAAGI+WF LMK+C+ KKI EFNP L+AIE
Sbjct: 421 LILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480

BLAST of Lcy03g008300 vs. ExPASy TrEMBL
Match: A0A6J1F0M1 (probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111441072 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.2e-244
Identity = 434/480 (90.42%), Postives = 459/480 (95.62%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGG 60
           MAD KQS NKS ++QP  DSSS SQTLPTT  TATS+ AAR+KLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAV +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGL 420
           VICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           IVMCL+PSAFLVV+MVFTHK V +VSA+MTAAGI+WF +MKIC+ KKI EFNP L+AIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Lcy03g008300 vs. ExPASy TrEMBL
Match: A0A0A0M300 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.6e-232
Identity = 421/479 (87.89%), Postives = 441/479 (92.07%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPY 60
           MAD KQ   +SN NQP    SS SQ LPTTT ++  + R+KLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MADDKQ---QSNSNQPATTDSS-SQILPTTTPSTPPSVRKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120
           GEEP VQAAGPLLAI+GFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS
Sbjct: 61  GEEPTVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120

Query: 121 LMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIV 180
           LMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAVLASTL+LA LNYIGLTIV
Sbjct: 121 LMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILAALNYIGLTIV 180

Query: 181 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLAFLSLLPFILMTFIAIPKI PHRWL LGDK  ++DWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300
           STLAGEVENPQKTFP ALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA
Sbjct: 241 STLAGEVENPQKTFPKALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300

Query: 301 GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLK LLEIGS+LSAIGLFEAQLSSSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVI
Sbjct: 301 GKWLKFLLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVI 360

Query: 361 CTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIV 420
           CTAISLAVSYM+F DIVASANFIYSLGMLLEFSSFVWLRW+HPG+KRPFKVPLKLPGLI+
Sbjct: 361 CTAISLAVSYMDFTDIVASANFIYSLGMLLEFSSFVWLRWRHPGIKRPFKVPLKLPGLIM 420

Query: 421 MCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEEL 480
           MCL+PS FLVVVMVFTHK V LVS  MT  GI+WF LMKIC+KKKI EFNP  EAI EL
Sbjct: 421 MCLIPSGFLVVVMVFTHKNVLLVSGGMTVGGILWFGLMKICKKKKILEFNPEPEAIVEL 473

BLAST of Lcy03g008300 vs. ExPASy TrEMBL
Match: A0A5A7UQT6 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00460 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.7e-231
Identity = 421/478 (88.08%), Postives = 441/478 (92.26%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPY 60
           MAD KQS   ++   PT DSS  SQ LPTTT ++  A R+KLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MADDKQS---NSSLPPTTDSS--SQILPTTTPSTPAAVRKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120
           GEEP VQAAGPLLAIVGFI+FPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS
Sbjct: 61  GEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120

Query: 121 LMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIV 180
           LMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAVLASTL+LALLNYIGLTIV
Sbjct: 121 LMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLTIV 180

Query: 181 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLAFLSLLPFILMTFIAIPKI PHRWL LGDK  ++DWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300
           STLAGEVENPQKTFP ALFVSVIFTCLSYL+PLLAVIGAVDVEQSAWGSGFHAQAAGIIA
Sbjct: 241 STLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300

Query: 301 GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGS+LSAIGLFEAQLSSSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVI
Sbjct: 301 GKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVI 360

Query: 361 CTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIV 420
           CTAISLAVSYM+F DIVASANFIYSLGMLLE SSFVWLRWKHPG+KRPFKVPLKLPGLIV
Sbjct: 361 CTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGLIV 420

Query: 421 MCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           MCL+P+ FLVVVMVFTHK V LVS  MT  GI+WF LMKIC+KKKI EFNP  EAI E
Sbjct: 421 MCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIVE 471

BLAST of Lcy03g008300 vs. ExPASy TrEMBL
Match: A0A1S3BNB6 (probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.7e-231
Identity = 421/478 (88.08%), Postives = 441/478 (92.26%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPY 60
           MAD KQS   ++   PT DSS  SQ LPTTT ++  A R+KLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MADDKQS---NSSLPPTTDSS--SQILPTTTPSTPAAVRKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120
           GEEP VQAAGPLLAIVGFI+FPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS
Sbjct: 61  GEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 120

Query: 121 LMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIV 180
           LMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAVLASTL+LALLNYIGLTIV
Sbjct: 121 LMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLTIV 180

Query: 181 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLAFLSLLPFILMTFIAIPKI PHRWL LGDK  ++DWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300
           STLAGEVENPQKTFP ALFVSVIFTCLSYL+PLLAVIGAVDVEQSAWGSGFHAQAAGIIA
Sbjct: 241 STLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 300

Query: 301 GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGS+LSAIGLFEAQLSSSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVI
Sbjct: 301 GKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVI 360

Query: 361 CTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIV 420
           CTAISLAVSYM+F DIVASANFIYSLGMLLE SSFVWLRWKHPG+KRPFKVPLKLPGLIV
Sbjct: 361 CTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGLIV 420

Query: 421 MCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           MCL+P+ FLVVVMVFTHK V LVS  MT  GI+WF LMKIC+KKKI EFNP  EAI E
Sbjct: 421 MCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIVE 471

BLAST of Lcy03g008300 vs. NCBI nr
Match: KAG6588239.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022154.1 putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 436/480 (90.83%), Postives = 459/480 (95.62%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGG 60
           MAD KQSLNKS ++QP  DSSS SQTLPTT  TATS+ AAR+KLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSLNKSTNSQPLADSSSPSQTLPTTVATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAV  STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFGSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGL 420
           VICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           IVMCL+PSAFLVV+MVFTHK V +VSAVMTAAGI+WF +MKIC+ KKI EFNP L+AIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Lcy03g008300 vs. NCBI nr
Match: XP_022933749.1 (probable polyamine transporter At3g13620 [Cucurbita moschata])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 434/480 (90.42%), Postives = 459/480 (95.62%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGG 60
           MAD KQS NKS ++QP  DSSS SQTLPTT  TATS+ AAR+KLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAV +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGL 420
           VICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           IVMCL+PSAFLVV+MVFTHK V +VSA+MTAAGI+WF +MKIC+ KKI EFNP L+AIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Lcy03g008300 vs. NCBI nr
Match: XP_023006107.1 (probable polyamine transporter At3g13620 [Cucurbita maxima])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 434/481 (90.23%), Postives = 459/481 (95.43%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTTTAT---SAIAARRKLTLIPLIFLIYFEVAG 60
           MAD KQS NKS ++QP  DSSS SQTLPTT+AT   SA A+R+KLTLIPLIFLIYFEVAG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPF 120
           GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGL 180
           WGSLMGTWKLLSGVINIAAFPVLCIDYIK+IAPPLESGWPRRIAV +STL+LALLNY+GL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240
           TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300
           DNVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAG
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 IIAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIG 360
           IIAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA RAKWFNTPWIG
Sbjct: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPWIG 360

Query: 361 IVICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPG 420
           IVICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP 
Sbjct: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420

Query: 421 LIVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIE 479
           LI+MCL+PSAFLVV+MVFTHK V +VSAVMTAAGI+WF LMK+C+ KKI EFNP L+AIE
Sbjct: 421 LILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480

BLAST of Lcy03g008300 vs. NCBI nr
Match: XP_023529220.1 (probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.1 bits (2195), Expect = 9.1e-243
Identity = 431/480 (89.79%), Postives = 457/480 (95.21%), Query Frame = 0

Query: 1   MADTKQSLNKSNHNQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGG 60
           MAD KQS NKS ++ P  DSSS SQTLPTT  TATS+ AAR+KLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+K+I PPLESGWPRRIAV +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGL 420
           VICTAISLAVSYMNF DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           IVMCL+PSAFLVV+MVFTHK V +VSA+MTAAGI+WF LMK+C+ KKI EFNP L+AIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480

BLAST of Lcy03g008300 vs. NCBI nr
Match: XP_038878316.1 (probable polyamine transporter At3g13620 [Benincasa hispida])

HSP 1 Score: 834.7 bits (2155), Expect = 4.0e-238
Identity = 434/479 (90.61%), Postives = 448/479 (93.53%), Query Frame = 0

Query: 1   MADTK-QSLNKSNHNQPTPDSSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGP 60
           MAD K QSLN  N NQP    SS +Q LPTTT  +  AAR+KLTLIPLIFLIYFEVAGGP
Sbjct: 1   MADKKQQSLNNFNSNQPATADSS-TQILPTTTTPAPAAARKKLTLIPLIFLIYFEVAGGP 60

Query: 61  YGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWG 120
           YGEEPAVQAAGPLLAI+GFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWG
Sbjct: 61  YGEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWG 120

Query: 121 SLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTI 180
           SLMGTWKLLSGVINIAAFPVLCIDYIK++APPLESGWPRRIA+LASTL+LALLNYIGLTI
Sbjct: 121 SLMGTWKLLSGVINIAAFPVLCIDYIKKMAPPLESGWPRRIAILASTLILALLNYIGLTI 180

Query: 181 VGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVAVVLAFLSLLPFILMTFI+IPKI PHRWL LGDK  K+DWNLYLNTLFWNLNFWDN
Sbjct: 181 VGYVAVVLAFLSLLPFILMTFISIPKIKPHRWLILGDK--KRDWNLYLNTLFWNLNFWDN 240

Query: 241 VSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGII 300
           VSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAA  I
Sbjct: 241 VSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAETI 300

Query: 301 AGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIV 360
           AGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIV
Sbjct: 301 AGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIV 360

Query: 361 ICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLI 420
           ICTAISLAVSYM+FADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLI
Sbjct: 361 ICTAISLAVSYMDFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLI 420

Query: 421 VMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEE 479
           VMCL+PSAFLVVVMVFTH  VFLVSA MT  GI+WF LMKIC+KKKI EFNP  EAI E
Sbjct: 421 VMCLIPSAFLVVVMVFTHTNVFLVSAAMTVGGILWFGLMKICKKKKILEFNPETEAIVE 476

BLAST of Lcy03g008300 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 636.7 bits (1641), Expect = 1.5e-182
Identity = 317/463 (68.47%), Postives = 385/463 (83.15%), Query Frame = 0

Query: 19  DSSSISQTLPTTTATSA--IAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIV 78
           ++S  S  LP TTA S+   A  +KLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLAI+
Sbjct: 5   EASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAIL 64

Query: 79  GFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGVINIAA 138
           GF++FPFIWS+PEALITAELSTAFPGNGGFVIWA RAFG F GS+MG+ K LSGVIN+A+
Sbjct: 65  GFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVAS 124

Query: 139 FPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLSLLPFI 198
           FPVLC+ Y+ ++ P LESGWPR + + AST+VL+ LNY GL IVGY AVVL  +SL PF+
Sbjct: 125 FPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFL 184

Query: 199 LMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPM 258
           +M+ +AIPKI PHRW SLG K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKTFP+
Sbjct: 185 VMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPL 244

Query: 259 ALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSSLSA 318
           AL ++VIFTC++YLIPL AV GAV V+QS W +GFHA+AA +IAGKWLKI +EIG+ LS+
Sbjct: 245 ALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSS 304

Query: 319 IGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYMNFADI 378
           IGLFEAQLSSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +SL +SYMNF DI
Sbjct: 305 IGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDI 364

Query: 379 VASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVVVMVFT 438
           ++SANF+Y+LGM LEF+SF+WLR K P +KRP++VPLK+PGL+VMCL+PSAFLV+++VF 
Sbjct: 365 ISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFA 424

Query: 439 HKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFNPRLEAIEEL 480
            K V+L+  VMT   I W+ L+   RK KIFEFN   E I++L
Sbjct: 425 TKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFN---EVIDDL 462

BLAST of Lcy03g008300 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.7e-117
Identity = 218/470 (46.38%), Postives = 316/470 (67.23%), Query Frame = 0

Query: 2   ADTKQSLNKSNHNQPTPD-SSSISQTLPTTTATSAIAARRKLTLIPLIFLIYFEVAGGPY 61
           A  K  ++  N   P P  S  ++   P T+    +   +K+T++PL+FLI++EV+GGP+
Sbjct: 12  ASQKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPF 71

Query: 62  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGS 121
           G E +V+AAGPLLAIVGFIVFPFIWS+PEALITAE+ T FP NGG+V+W   A GP+WG 
Sbjct: 72  GIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGF 131

Query: 122 LMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIV 181
             G  K LSGVI+ A +P+L +DY+K   P L SG PR  A+L  T+ L  LNY GL+IV
Sbjct: 132 QQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIV 191

Query: 182 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNV 241
           G  AV+L   S+LPF++M+F++IPK+ P RWL +  K    +W+LYLNTLFWNLN+WD+V
Sbjct: 192 GVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSV 251

Query: 242 STLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 301
           STL GEVENP KT P ALF +++    SY+ P+L   GA+ ++Q  W  G+ A    +I 
Sbjct: 252 STLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIG 311

Query: 302 GKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 361
           G WL   ++  ++ S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TPW+GI+ 
Sbjct: 312 GVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILF 371

Query: 362 CTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIV 421
             +  + +S+++F +IVA+ N +Y  GM+LEF +FV LR K+P   RPFK+P+ + G ++
Sbjct: 372 SASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVL 431

Query: 422 MCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFN 471
           MC+ P+  + V+M FT+  V LVS      G++    +K   KK   +F+
Sbjct: 432 MCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Lcy03g008300 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 1.0e-114
Identity = 216/459 (47.06%), Postives = 314/459 (68.41%), Query Frame = 0

Query: 14  NQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGP 73
           N   P SS  +  +P++   AT  I   RK++++PL+FLI++EV+GGP+G E +V AAGP
Sbjct: 22  NNEVPYSSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGP 81

Query: 74  LLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGV 133
           LLA++GF++FPFIWS+PEALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGV
Sbjct: 82  LLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGV 141

Query: 134 INIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLS 193
           I+ A +PVL +DY+K   P L SG PR  ++L  T++L  LNY GLTIVG+VAV++   S
Sbjct: 142 IDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFS 201

Query: 194 LLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQ 253
           +LPF +M  I+IP++ P RWL + D G   +WNLYLNTLFWNLN+WD++STLAGEVENP 
Sbjct: 202 ILPFAVMGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPN 261

Query: 254 KTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIG 313
            T P ALF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  
Sbjct: 262 HTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAA 321

Query: 314 SSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYM 373
           ++ S +G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S++
Sbjct: 322 AATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWL 381

Query: 374 NFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVV 433
           +F +IVA+ N +Y +GM+LEF +FV +R KHP   RP+K+P+   G I+MC+ P+  +  
Sbjct: 382 SFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICA 441

Query: 434 VMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFN 471
           V+  +   V  VS VM   G +   L+    +K+  +F+
Sbjct: 442 VVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 474

BLAST of Lcy03g008300 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 1.0e-114
Identity = 216/459 (47.06%), Postives = 314/459 (68.41%), Query Frame = 0

Query: 14  NQPTPDSSSISQTLPTT--TATSAIAARRKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGP 73
           N   P SS  +  +P++   AT  I   RK++++PL+FLI++EV+GGP+G E +V AAGP
Sbjct: 6   NNEVPYSSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGP 65

Query: 74  LLAIVGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGV 133
           LLA++GF++FPFIWS+PEALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGV
Sbjct: 66  LLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGV 125

Query: 134 INIAAFPVLCIDYIKRIAPPLESGWPRRIAVLASTLVLALLNYIGLTIVGYVAVVLAFLS 193
           I+ A +PVL +DY+K   P L SG PR  ++L  T++L  LNY GLTIVG+VAV++   S
Sbjct: 126 IDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFS 185

Query: 194 LLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQ 253
           +LPF +M  I+IP++ P RWL + D G   +WNLYLNTLFWNLN+WD++STLAGEVENP 
Sbjct: 186 ILPFAVMGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPN 245

Query: 254 KTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIG 313
            T P ALF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  
Sbjct: 246 HTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAA 305

Query: 314 SSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISLAVSYM 373
           ++ S +G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S++
Sbjct: 306 AATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWL 365

Query: 374 NFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVV 433
           +F +IVA+ N +Y +GM+LEF +FV +R KHP   RP+K+P+   G I+MC+ P+  +  
Sbjct: 366 SFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICA 425

Query: 434 VMVFTHKTVFLVSAVMTAAGIIWFVLMKICRKKKIFEFN 471
           V+  +   V  VS VM   G +   L+    +K+  +F+
Sbjct: 426 VVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 458

BLAST of Lcy03g008300 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.7e-112
Identity = 211/433 (48.73%), Postives = 297/433 (68.59%), Query Frame = 0

Query: 41  KLTLIPLIFLIYFEVAGGPYGEEPAVQA-AGPLLAIVGFIVFPFIWSVPEALITAELSTA 100
           KLTL+PL+FLI++EV+GGP+G E +V++  GPLLA++GF++FP IWS+PEAL+TAEL+T+
Sbjct: 21  KLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELATS 80

Query: 101 FPGNGGFVIWAERAFGPFWGSLMGTWKLLSGVINIAAFPVLCIDYIKRIAPPLESGWPRR 160
           FP NGG+V+W   AFGPFWG   G WK  SGV++ A +PVL +DY+K   P L+    R 
Sbjct: 81  FPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAARV 140

Query: 161 IAVLASTLVLALLNYIGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGV 220
            A+L  T  L  LNY GL IVG+ AVVLA  SL PF++M  +A+P I P RWL +  +  
Sbjct: 141 PALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ-- 200

Query: 221 KKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGA 280
           K +W  Y NT+FWNLN+WD  STLAGEV+ P KTFP ALF +V+    SYLIPL+A  GA
Sbjct: 201 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 260

Query: 281 VDVEQSA-WGSGFHAQAAGIIAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIG 340
           +    S  W  G+ A+   +I G WLK  ++  +++S +GLFEA++SS A+Q+LGM+EIG
Sbjct: 261 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 320

Query: 341 ILPKFFAARAKWFNTPWIGIVICTAISLAVSYMNFADIVASANFIYSLGMLLEFSSFVWL 400
           +LP FFA R+K + TP I I+      + +S+M+F +I+   NF+Y+LGMLLEF++FV L
Sbjct: 321 MLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKL 380

Query: 401 RWKHPGMKRPFKVPLKLPGLIVMCLVPSAFLVVVMVFTHKTVFLVSAVMTAAGIIWFVLM 460
           R K P + RP++VPL   G+ ++CL PS  +++VMV      FL+S V+   G   +  +
Sbjct: 381 RIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFL 440

Query: 461 KICRKKKIFEFNP 472
            + ++K+   F P
Sbjct: 441 TLVKEKQWARFIP 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN72.1e-18168.47Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL12.3e-11646.38Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9C6S51.4e-11347.06Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M81.9e-11347.90Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D01.9e-11347.90Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1KV065.2e-24490.23probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1F0M15.2e-24490.42probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A0A0M3004.6e-23287.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1[more]
A0A5A7UQT61.7e-23188.08Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BNB61.7e-23188.08probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953... [more]
Match NameE-valueIdentityDescription
KAG6588239.11.7e-24490.83putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_022933749.11.1e-24390.42probable polyamine transporter At3g13620 [Cucurbita moschata][more]
XP_023006107.11.1e-24390.23probable polyamine transporter At3g13620 [Cucurbita maxima][more]
XP_023529220.19.1e-24389.79probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo][more]
XP_038878316.14.0e-23890.61probable polyamine transporter At3g13620 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G13620.11.5e-18268.47Amino acid permease family protein [more]
AT5G05630.11.7e-11746.38Amino acid permease family protein [more]
AT1G31830.11.0e-11447.06Amino acid permease family protein [more]
AT1G31830.21.0e-11447.06Amino acid permease family protein [more]
AT3G19553.12.7e-11248.73Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 64..450
e-value: 3.9E-33
score: 115.0
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 18..477
e-value: 1.4E-62
score: 209.2
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 38..475
e-value: 2.4E-60
score: 206.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 20..473
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 20..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g008300.1Lcy03g008300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity