Lcy03g008160 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g008160
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr03: 39869123 .. 39869716 (+)
RNA-Seq Expression	Lcy03g008160
Synteny	Lcy03g008160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTGGTCTACTGGCATGTTGTTCTCTACTGTTCTGCATTAGAATTTGTCGATACCTATGAATTAGAATCAAAATCAAAATGGATAACTCCCGGTCCGATTAGAATCGAGAAAGATGGGGGTGCCCTCGATTCGAGAAAGATGGGGGTGCCCTCGATTCGAGAACCTCTTGAGTCAAGCAAAATGTACTCCTTCCCTCGCCCCCCAAATTGTTGGAAATTCAATTTGGATACCTCTTGGAGCACTGTTGAGGAGATTAGTGGGTTGAGATGGGTCATGCAGGACTCCGACGAGTCTCTTATCTGTGCGAGCCTGCAACCAATCAACGAAAAGTGGCCGATCAAGGTCCTCAAGACGAAAGCAATGTGGGAAGGTTTAAAGAACTTAGATGCAATTTATGGGGGTTTGCTGATTCTTGAAGTCGAGTCGGATTGCCTCGACCTTATCCGCTGCTTGAATAACGATGATGTAGATCTCTTGGAGTTGAAGGTTATTGTTGAAGCCATCTTTGATTTGGCGAAAAAGATAGGGGTGCTCTCATTTAAGCATTGCCCTGGAATCCAAAAATGGAGTGACACATTCCATCGCTAG

mRNA sequence

ATGGAAGTGGTCTACTGGCATGTTGTTCTCTACTGTTCTGCATTAGAATTTGTCGATACCTATGAATTAGAATCAAAATCAAAATGGATAACTCCCGGTCCGATTAGAATCGAGAAAGATGGGGGTGCCCTCGATTCGAGAAAGATGGGGGTGCCCTCGATTCGAGAACCTCTTGAGTCAAGCAAAATGTACTCCTTCCCTCGCCCCCCAAATTGTTGGAAATTCAATTTGGATACCTCTTGGAGCACTGTTGAGGAGATTAGTGGGTTGAGATGGGTCATGCAGGACTCCGACGAGTCTCTTATCTGTGCGAGCCTGCAACCAATCAACGAAAAGTGGCCGATCAAGGTCCTCAAGACGAAAGCAATGTGGGAAGGTTTAAAGAACTTAGATGCAATTTATGGGGGTTTGCTGATTCTTGAAGTCGAGTCGGATTGCCTCGACCTTATCCGCTGCTTGAATAACGATGATGTAGATCTCTTGGAGTTGAAGGTTATTGTTGAAGCCATCTTTGATTTGGCGAAAAAGATAGGGGTGCTCTCATTTAAGCATTGCCCTGGAATCCAAAAATGGAGTGACACATTCCATCGCTAG

Coding sequence (CDS)

ATGGAAGTGGTCTACTGGCATGTTGTTCTCTACTGTTCTGCATTAGAATTTGTCGATACCTATGAATTAGAATCAAAATCAAAATGGATAACTCCCGGTCCGATTAGAATCGAGAAAGATGGGGGTGCCCTCGATTCGAGAAAGATGGGGGTGCCCTCGATTCGAGAACCTCTTGAGTCAAGCAAAATGTACTCCTTCCCTCGCCCCCCAAATTGTTGGAAATTCAATTTGGATACCTCTTGGAGCACTGTTGAGGAGATTAGTGGGTTGAGATGGGTCATGCAGGACTCCGACGAGTCTCTTATCTGTGCGAGCCTGCAACCAATCAACGAAAAGTGGCCGATCAAGGTCCTCAAGACGAAAGCAATGTGGGAAGGTTTAAAGAACTTAGATGCAATTTATGGGGGTTTGCTGATTCTTGAAGTCGAGTCGGATTGCCTCGACCTTATCCGCTGCTTGAATAACGATGATGTAGATCTCTTGGAGTTGAAGGTTATTGTTGAAGCCATCTTTGATTTGGCGAAAAAGATAGGGGTGCTCTCATTTAAGCATTGCCCTGGAATCCAAAAATGGAGTGACACATTCCATCGCTAG

Protein sequence

MEVVYWHVVLYCSALEFVDTYELESKSKWITPGPIRIEKDGGALDSRKMGVPSIREPLESSKMYSFPRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPIKVLKTKAMWEGLKNLDAIYGGLLILEVESDCLDLIRCLNNDDVDLLELKVIVEAIFDLAKKIGVLSFKHCPGIQKWSDTFHR

Homology

[Close]

BLAST of Lcy03g008160 vs. ExPASy TrEMBL
Match: A0A5D3E6P6 (RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005870 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.4e-07
Identity = 32/72 (44.44%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 56  EPLESSKMYSFPRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPI 115
           E   S K++  P     W+FNLD SW+ VEEISG  WV++DS  + I AS +    KWP+
Sbjct: 4   ESTRSQKIWMSPY-VGFWRFNLDASWNDVEEISGTSWVVRDSAGAFILASCERAEVKWPM 63

Query: 116 KVLKTKAMWEGL 128
           +VL+  A   GL
Sbjct: 64  EVLEALATNRGL 74

[Close]

BLAST of Lcy03g008160 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.4e-07
Identity = 36/110 (32.73%), Postives = 55/110 (50.00%), Query Frame = 0


Query: 51  VPSIREPLESSKMYSFPRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPIN 110
           +P  RE L    +     P NCWK N D SWS   E+ G+ W++ D    ++ A    I 
Sbjct: 91  LPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIR 150

Query: 111 EKWPIKVLKTKAMWEGLKNLDAIYGGLLILEVESDCLDLIRCLNNDDVDL 161
           EK  I  L+   +  GL+ ++      + L  ESD +++IR +  +DVDL
Sbjct: 151 EKKEINALELMTIIRGLQFINMQSRSPIYL--ESDSVEVIRLMKKEDVDL 198

[Close]

BLAST of Lcy03g008160 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.7e-05
Identity = 35/121 (28.93%), Postives = 57/121 (47.11%), Query Frame = 0


Query: 67  PRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPIKVLKTKAMWE- 126
           P   + WK N+D +W       GL W+++DS+   I A          +K L      E 
Sbjct: 80  PPAQHLWKLNVDATWMDSLHAGGLGWIVRDSEGRFIMAEC--------LKALSQSLTLEA 139

Query: 127 GLKNLDAIYGGLLILEVESDCLDLIRCLNNDDVDLLELKVIVEAIFDLAKKIGVLSFKHC 186
           G+K           +E+ESDCL+++  +N   + L E+ +IVE I+   + + +  FKH 
Sbjct: 140 GIK-----------IEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGFKHL 181

[Close]

BLAST of Lcy03g008160 vs. ExPASy TrEMBL
Match: A0A2I4H9M5 (uncharacterized protein LOC109014810 OS=Juglans regia OX=51240 GN=LOC109014810 PE=4 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-04
Identity = 36/124 (29.03%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 63  MYSFPRPP-NCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPIKVLKTK 122
           +Y++ RPP N +K N D +    +  +G+  +++DS   L  A  +   E   +++++  
Sbjct: 347 LYTWSRPPANRFKLNCDGALFFDQNKTGIGAILRDSHGFLFMALSRGEEEFIELEIVEAT 406

Query: 123 AMWEGLKNLDAIYGGLLILEVESDCLDLIRCLNNDDVDLLELKVIVEAIFDLAKKIGVLS 182
           A+  GL+    I  G+  LE+ESDC  L+  +NN+ +  + ++ I+  I  L K  G   
Sbjct: 407 AVLRGLQFYLNI--GIQNLEIESDCQYLVEEVNNETISYIAMRKIIIEIRRLMKSFGDCK 466

Query: 183 FKHC 186
           F+HC
Sbjct: 467 FQHC 468

[Close]

BLAST of Lcy03g008160 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 80.1 bits (196), Expect = 2.4e-11
Identity = 50/123 (40.65%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 74  KFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPIKVLKTKAMWEGLKNLDAI 133
           K N   SWS      G+ WV+ DS  S IC   + +  KW +K+L+ KAM EGLK     
Sbjct: 7   KLNSYASWSEEAGKGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTY--- 66

Query: 134 YGGLLILEVESDC------LDLIRCLNNDDVDLLELKVIVEAIFDLAKKIGVLSFKHCPG 191
              LLI E  S        L+L R LNN  +DL+EL  +V+ IFDL    G++SF   P 
Sbjct: 67  ---LLIRETVSYSHRPIIFLELARILNNTHMDLIELSNVVDEIFDLELLAGIISFFQVPS 123

[Close]

BLAST of Lcy03g008160 vs. NCBI nr
Match: XP_038886170.1 (uncharacterized protein LOC120076417 [Benincasa hispida])

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-08
Identity = 47/146 (32.19%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 41  GGALDSRKMGVPSIREPLESSKMYSF-PRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDE 100
           G  LD +  G   +  P+++  +  + P  P  WK N+D SW++  +  GL WV  D   
Sbjct: 47  GEHLDRKGKG---LYHPIQAPTLVGWHPSKPFYWKLNMDASWNSKIDACGLGWVFLDHLY 106

Query: 101 SLICASLQPINEKWPIKVLKTKAMWEGLKNLDAIYGGLLILEVESDCL-DLIRCLNNDDV 160
            +  A L+ +     + +L+  A+  GL+ L +I   +  + VESDCL ++I  LN+D V
Sbjct: 107 RVCMAGLKFVLRCQKVNILEAIAICFGLEILSSI--DISNIMVESDCLEEVINLLNDDVV 166

Query: 161 DLLELKVIVEAIFDLAKKIGVLSFKH 185
           DL E+    E   D    +GV+SF H
Sbjct: 167 DLSEVSFCSEEAKDRGGNLGVISFSH 187

[Close]

BLAST of Lcy03g008160 vs. NCBI nr
Match: KAA0038679.1 (hypothetical protein E6C27_scaffold92G002210 [Cucumis melo var. makuwa] >TYK31290.1 hypothetical protein E5676_scaffold455G005870 [Cucumis melo var. makuwa])

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-06
Identity = 32/72 (44.44%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 56  EPLESSKMYSFPRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPI 115
           E   S K++  P     W+FNLD SW+ VEEISG  WV++DS  + I AS +    KWP+
Sbjct: 4   ESTRSQKIWMSPY-VGFWRFNLDASWNDVEEISGTSWVVRDSAGAFILASCERAEVKWPM 63

Query: 116 KVLKTKAMWEGL 128
           +VL+  A   GL
Sbjct: 64  EVLEALATNRGL 74

[Close]

BLAST of Lcy03g008160 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-06
Identity = 36/110 (32.73%), Postives = 55/110 (50.00%), Query Frame = 0


Query: 51  VPSIREPLESSKMYSFPRPPNCWKFNLDTSWSTVEEISGLRWVMQDSDESLICASLQPIN 110
           +P  RE L    +     P NCWK N D SWS   E+ G+ W++ D    ++ A    I 
Sbjct: 91  LPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIR 150

Query: 111 EKWPIKVLKTKAMWEGLKNLDAIYGGLLILEVESDCLDLIRCLNNDDVDL 161
           EK  I  L+   +  GL+ ++      + L  ESD +++IR +  +DVDL
Sbjct: 151 EKKEINALELMTIIRGLQFINMQSRSPIYL--ESDSVEVIRLMKKEDVDL 198

[Close]

BLAST of Lcy03g008160 vs. NCBI nr
Match: XP_015384866.1 (uncharacterized protein LOC107176592 [Citrus sinensis] >XP_024953719.1 uncharacterized protein LOC107176592 [Citrus sinensis])

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-06
Identity = 44/169 (26.04%), Postives = 84/169 (49.70%), Query Frame = 0


Query: 18  VDTYELESKSKWITPGPIRIEKDGGALDSRKMGVPSIREPLESSKMYSFPRPPNCWKFNL 77
           +D + + +K++ +     R+ + G        G   +  P++S K    P P N +K N+
Sbjct: 1   MDCWRVVAKAEAVVEAYKRVSQQG-------HGSGGMHNPMKSQKW--CPPPANVFKANV 60

Query: 78  DTSWSTVEEISGLRWVMQDSDESLICASLQPINEKWPIKVLKTKAMWEGLKNLDAIYGGL 137
           D + +  + I+GL  V++DS+  +I A+++       +KV + +A+  GL  + A    L
Sbjct: 61  DAAINHEKGIAGLGAVIRDSNSRIIVAAVKTTKFSGDVKVAEAEAVEWGL--VVAKNAAL 120

Query: 138 LILEVESDCLDLIRCLNNDDVDLLELKVIVEAIFDLAKKIGVLSFKHCP 187
             L VE+DC D+ + +NN +    E+  ++  I D  K    +SF + P
Sbjct: 121 SSLMVETDCQDVAKLINNQEGSKTEIVWVISEIQDQRKDFQHISFHYTP 158

The following BLAST results are available for this feature:

BLAST of Lcy03g008160 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy03g008160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E6P6	8.4e-07	44.44	RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1CQG0	8.4e-07	32.73	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1D4B6	2.7e-05	28.93	uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A2I4H9M5	1.8e-04	29.03	uncharacterized protein LOC109014810 OS=Juglans regia OX=51240 GN=LOC109014810 P...	[more]

BLAST of Lcy03g008160 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6599977.1	2.4e-11	40.65	hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038886170.1	5.4e-08	32.19	uncharacterized protein LOC120076417 [Benincasa hispida]	[more]
KAA0038679.1	1.7e-06	44.44	hypothetical protein E6C27_scaffold92G002210 [Cucumis melo var. makuwa] >TYK3129...	[more]
XP_022143317.1	1.7e-06	32.73	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_015384866.1	6.6e-06	26.04	uncharacterized protein LOC107176592 [Citrus sinensis] >XP_024953719.1 uncharact...	[more]

BLAST of Lcy03g008160 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 76..186 e-value: 3.3E-9 score: 36.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g008160.1	Lcy03g008160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity