Lcy03g006790 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g006790
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description(1->3)-beta-glucan endohydrolase
LocationChr03: 35976429 .. 35977529 (-)
RNA-Seq ExpressionLcy03g006790
SyntenyLcy03g006790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAAATAGAAAACACATCCAAAATTCCGAAACAAGGTACACATAAACTAATCATTTGTCCATCTTATATTTTTCCAATTCTTATAAATCCAATAATCGACAAGCAATGTGTCAACGAGATTAACGAAAGTTTTGTCCAATTTCATGTAGTGGCTCAAATCGGTGTATGTTATGGACAAAATGGCAATGACCTACCATCTCCACCCGAAGTTATCGACCTTTACAATCAAAACAACATCCAAAGAATGCGACTTTACACTCCAAACCAACCTACTTTCGATGCTCTTCGAGGCTCCAAAATCGAGCTCATGCTCGATCTCCCCAACGACCAAATTCAAGCCATGGCTGCATCCCAAGACAATGCTAATTCATGGGTTCAAGACAACATCCTCAACCATGGCGACATCAAATTCAAGTACGTCGTTGTCGGAAACGAGATAAAAACTAATGATGAAGCTGCTAGCTTCCTCGTACCCACCATGCGAAACATCCAAAATGCAATTTCAGCTATGGGCTTGAGAGATCAAATCAAAGTTTCAACTGCATTCGACACTGGAATTCTTTTAGAATCTTCCCCTCCTTCAAAGGGCTCATTCAGAAGTGACTATCTTCCAATTCTTGACCCGACCATTCGCTTTCTTAACGACAACAACTCCCCATTTCTTGTCAATGTGTACCCTTACTTCAGTTATATCAACACTCCAAACATGGCACTCGATTTTGCGCTTTTTACTGGGCCGTCATCGGTGGTTGTAGATGATCAACTTGCTTACCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGATAAGTCTAATGGCGGAGGATTGGAAATTGTCGTGTCGGAGACTGGCTGGCCGACCGAGGGTGGGCAGGCGGCCACGGTAGACAATGCGAGTGCTTACAACAATAATTTGATTCAACATGTGAAGGTGGGGACACCGAAGAGACCTGGAAGGCCGATTGAGACCTATGTGTTTGCGATGTTTGATGAGAATGAGAAGAAGACGCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAAATTAAATATCAGGTCAACTTCAACTAA

mRNA sequence

ATGTTTAAAATAGAAAACACATCCAAAATTCCGAAACAAGGTACACATAAACTAATCATTTGTCCATCTTATATTTTTCCAATTCTTATAAATCCAATAATCGACAAGCAATGTGTCAACGAGATTAACGAAAGTTTTGTCCAATTTCATGTAGTGGCTCAAATCGGTGTATGTTATGGACAAAATGGCAATGACCTACCATCTCCACCCGAAGTTATCGACCTTTACAATCAAAACAACATCCAAAGAATGCGACTTTACACTCCAAACCAACCTACTTTCGATGCTCTTCGAGGCTCCAAAATCGAGCTCATGCTCGATCTCCCCAACGACCAAATTCAAGCCATGGCTGCATCCCAAGACAATGCTAATTCATGGGTTCAAGACAACATCCTCAACCATGGCGACATCAAATTCAAGTACGTCGTTGTCGGAAACGAGATAAAAACTAATGATGAAGCTGCTAGCTTCCTCGTACCCACCATGCGAAACATCCAAAATGCAATTTCAGCTATGGGCTTGAGAGATCAAATCAAAGTTTCAACTGCATTCGACACTGGAATTCTTTTAGAATCTTCCCCTCCTTCAAAGGGCTCATTCAGAAGTGACTATCTTCCAATTCTTGACCCGACCATTCGCTTTCTTAACGACAACAACTCCCCATTTCTTGTCAATGTGTACCCTTACTTCAGTTATATCAACACTCCAAACATGGCACTCGATTTTGCGCTTTTTACTGGGCCGTCATCGGTGGTTGTAGATGATCAACTTGCTTACCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGATAAGTCTAATGGCGGAGGATTGGAAATTGTCGTGTCGGAGACTGGCTGGCCGACCGAGGGTGGGCAGGCGGCCACGGTAGACAATGCGAGTGCTTACAACAATAATTTGATTCAACATGTGAAGGTGGGGACACCGAAGAGACCTGGAAGGCCGATTGAGACCTATGTGTTTGCGATGTTTGATGAGAATGAGAAGAAGACGCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAAATTAAATATCAGGTCAACTTCAACTAA

Coding sequence (CDS)

ATGTTTAAAATAGAAAACACATCCAAAATTCCGAAACAAGGTACACATAAACTAATCATTTGTCCATCTTATATTTTTCCAATTCTTATAAATCCAATAATCGACAAGCAATGTGTCAACGAGATTAACGAAAGTTTTGTCCAATTTCATGTAGTGGCTCAAATCGGTGTATGTTATGGACAAAATGGCAATGACCTACCATCTCCACCCGAAGTTATCGACCTTTACAATCAAAACAACATCCAAAGAATGCGACTTTACACTCCAAACCAACCTACTTTCGATGCTCTTCGAGGCTCCAAAATCGAGCTCATGCTCGATCTCCCCAACGACCAAATTCAAGCCATGGCTGCATCCCAAGACAATGCTAATTCATGGGTTCAAGACAACATCCTCAACCATGGCGACATCAAATTCAAGTACGTCGTTGTCGGAAACGAGATAAAAACTAATGATGAAGCTGCTAGCTTCCTCGTACCCACCATGCGAAACATCCAAAATGCAATTTCAGCTATGGGCTTGAGAGATCAAATCAAAGTTTCAACTGCATTCGACACTGGAATTCTTTTAGAATCTTCCCCTCCTTCAAAGGGCTCATTCAGAAGTGACTATCTTCCAATTCTTGACCCGACCATTCGCTTTCTTAACGACAACAACTCCCCATTTCTTGTCAATGTGTACCCTTACTTCAGTTATATCAACACTCCAAACATGGCACTCGATTTTGCGCTTTTTACTGGGCCGTCATCGGTGGTTGTAGATGATCAACTTGCTTACCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGATAAGTCTAATGGCGGAGGATTGGAAATTGTCGTGTCGGAGACTGGCTGGCCGACCGAGGGTGGGCAGGCGGCCACGGTAGACAATGCGAGTGCTTACAACAATAATTTGATTCAACATGTGAAGGTGGGGACACCGAAGAGACCTGGAAGGCCGATTGAGACCTATGTGTTTGCGATGTTTGATGAGAATGAGAAGAAGACGCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAAATTAAATATCAGGTCAACTTCAACTAA

Protein sequence

MFKIENTSKIPKQGTHKLIICPSYIFPILINPIIDKQCVNEINESFVQFHVVAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSYINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWGLFSPNKQIKYQVNFN
Homology
BLAST of Lcy03g006790 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 9.5e-110
Identity = 194/318 (61.01%), Postives = 241/318 (75.79%), Query Frame = 0

Query: 50  HVVAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLP 109
           H  A IGVC G  G+DLP   EV+ LY  NNI RMRLY PN    +ALRGS I+L+L +P
Sbjct: 35  HTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVP 94

Query: 110 NDQIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAI 169
           N+ +Q +A SQ NAN+WVQ+N+ N+ ++KFKY+ VGNE+K +D  A FLVP MRNIQ AI
Sbjct: 95  NENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAI 154

Query: 170 SAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPY 229
           S  GL  +IKVSTA DTG+L E+ PPS GSF+S+Y  +L P IRFL  + SP LVN+YPY
Sbjct: 155 SLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPY 214

Query: 230 FSYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSE 289
           F+Y  NT ++ LD+ALFT PS VV D    Y+NLFDA+LD VY+AL+K+ GG L++V+SE
Sbjct: 215 FAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISE 274

Query: 290 TGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEK 349
           TGWP+  G A T+DNA  + +NLIQHVK GTP+RPGRPIETY+FAMFDEN K   PE+EK
Sbjct: 275 TGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRK--TPELEK 334

Query: 350 HWGLFSPNKQIKYQVNFN 367
           HWGLFSP KQ KYQ++FN
Sbjct: 335 HWGLFSPTKQPKYQISFN 350

BLAST of Lcy03g006790 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.5e-107
Identity = 196/318 (61.64%), Postives = 244/318 (76.73%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQ GVCYG+ GN+LP+P EV+ LYNQ NI+RMR+Y P+    +ALRGS IEL+LD+PND 
Sbjct: 32  AQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPNDN 91

Query: 113 IQAMAASQDNANSWVQDNILNH-GDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISA 172
           ++ +A+SQDNAN WVQDNI N+  +++F+YV VGNE+K     A FLVP + NIQ AIS 
Sbjct: 92  LRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAISN 151

Query: 173 MGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYL-PILDPTIRFLNDNNSPFLVNVYPYF 232
            GL +Q+KVSTA DTG L ES PPSKGSF+SDY    LD  IRFL +NN+P +VNVY YF
Sbjct: 152 AGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYSYF 211

Query: 233 SYINTP-NMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSET 292
           +Y   P +++LD+ALF  PS VV D  L Y+NLFDA +DAVY+AL+K+ GG L IVVSE+
Sbjct: 212 AYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVSES 271

Query: 293 GWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKH 352
           GWP+ GG A ++DNA  YN NL+++VK GTPKRPG P+ETYVFAMFDEN+K+  PE EK 
Sbjct: 272 GWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQKQ--PEFEKF 331

Query: 353 WGLFSP-NKQIKYQVNFN 367
           WGLFSP  KQ KY +NFN
Sbjct: 332 WGLFSPITKQPKYSINFN 347

BLAST of Lcy03g006790 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.4e-107
Identity = 187/314 (59.55%), Postives = 237/314 (75.48%), Query Frame = 0

Query: 54  QIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQI 113
           Q+GVCYG+NGN+LPSP E I L+ Q NIQR+RLY+P+     ALRGS IE+ L LPN  +
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 114 QAMAASQDNANSWVQDNILNHGD-IKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 173
           Q++A+SQ  AN+WVQ  ++N+ + ++F+Y+ VGNE+K +D  A FLVP M NI  A+ A 
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 174 GLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSY 233
           GL  +IKVST+ D G+L ES PPSKGSFR D + +++P IRFL   NSP L+N+Y YFSY
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 234 I-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 293
             N   + LD+ALFT PS +V D   +YQNLFDA+LDA+YSAL+KS G  LEIVV+ETGW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 294 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 353
           PT GG    ++NA  YNNNLI+HVK GTPKRPG+ IETY+FA++DEN+K TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 354 LFSPNKQIKYQVNF 366
           LF PNKQ KY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

BLAST of Lcy03g006790 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.9e-102
Identity = 182/318 (57.23%), Postives = 240/318 (75.47%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQ GVCYG+ GN LPSP +V+ L N+NNI+RMR+Y P+QPT +ALRGS IELML +PN  
Sbjct: 24  AQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTLEALRGSNIELMLGVPNPD 83

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASF---LVPTMRNIQNAI 172
           ++ +AASQ NA++WVQ+N+ N+G++KF+Y+ VGNE+   +E + +   L+  MRNIQ AI
Sbjct: 84  LENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNENSKYVPVLLNAMRNIQTAI 143

Query: 173 SAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPY 232
           S  GL +QIKVSTA +TG+  ++SPPS G F+ D    ++P I FL  N +P LVN+YPY
Sbjct: 144 SGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPIINFLVTNRAPLLVNLYPY 203

Query: 233 FSYINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSET 292
           F+  N  ++ L++ALFT    VV D+   Y+NLFDAILDA YSAL+K++G  LEIVVSE+
Sbjct: 204 FAIANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYSALEKASGSSLEIVVSES 263

Query: 293 GWPTEG-GQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEK 352
           GWP+ G GQ  ++DNA  YNNNLI HVK G+PKRP  PIETYVFA+FDE++K   PE+EK
Sbjct: 264 GWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIETYVFALFDEDQK--DPEIEK 323

Query: 353 HWGLFSPNKQIKYQVNFN 367
           H+GLFS N Q KYQ++FN
Sbjct: 324 HFGLFSANMQPKYQISFN 339

BLAST of Lcy03g006790 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 8.3e-98
Identity = 183/317 (57.73%), Postives = 233/317 (73.50%), Query Frame = 0

Query: 52  VAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPND 111
           V QIGVCYG+NGN+L    EV+ LY Q NI+RMRLY PNQ T +ALRGS IEL+LD+PN 
Sbjct: 32  VGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNP 91

Query: 112 QIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISA 171
            +Q +A+SQ  A++WV++N+ N+ ++ F+Y+ VGNE++ +D+AASF++P M+NI+ A+S+
Sbjct: 92  DLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSS 151

Query: 172 MGLRDQIKVSTAFDT-GILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYF 231
           +G    IKVSTA DT GI     PPS G+F  ++   + P I FL+   SP LVN YPYF
Sbjct: 152 LG----IKVSTAIDTRGI--SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYF 211

Query: 232 SYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSET 291
           SY  N  ++ LD+ LFT PS+VV D Q  Y+NLF AILD VY++L+K+ GG LEIVVSE+
Sbjct: 212 SYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSES 271

Query: 292 GWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKH 351
           GWPT GG A  VDNA  Y NNLIQ VK G+P+RPGR  ETY+FAMFDEN K+  PE EK 
Sbjct: 272 GWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQ-GPETEKF 331

Query: 352 WGLFSPNKQIKYQVNFN 367
           WGLF PN Q KY VNFN
Sbjct: 332 WGLFLPNLQPKYVVNFN 341

BLAST of Lcy03g006790 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 5.6e-137
Identity = 245/315 (77.78%), Postives = 277/315 (87.94%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQIGVCYGQ G+DLPSP EVIDLYNQNNI+RMRLY PNQP+ +ALRGS IELML +PN +
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 172
           ++AMA +Q+NANSW QDN+L+ GD+KFKY+VVGNEIK +D AA FLVP M+NIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 173 GLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSY 232
           GLR +IKVSTA DTG+L ES PPSKGSFRSDYLPILDPTIRFL DNNSP L+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 233 I-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 292
           I NT +++LD+ALFTG  S+V D QL YQNLFDAILDAVYSAL+KS GGGLEIVVSE+GW
Sbjct: 212 ISNTKDISLDYALFTG-MSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 271

Query: 293 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 352
           PT+GG+AA VDNA  YNNNLIQHVK GTPKRPG PIETYVFAMFDEN+K TPPEVEKHWG
Sbjct: 272 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 331

Query: 353 LFSPNKQIKYQVNFN 367
           LFSPNKQ KY VNF+
Sbjct: 332 LFSPNKQPKYLVNFH 345

BLAST of Lcy03g006790 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 7.2e-137
Identity = 245/309 (79.29%), Postives = 272/309 (88.03%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQIGVCYGQ GNDLPSP EVIDLYNQNNIQRMRLY PNQ TF+ALRGS IELML LPNDQ
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPNDQ 92

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 172
           IQ+MAA+QDNAN+W+QDNILN  D+ FKY+VVGNEIKTN+EAA FLVP M+NIQNAISA+
Sbjct: 93  IQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 173 GLRDQIKVSTAFDTGIL-LESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFS 232
           GL+ QIKVSTAF TGIL  ES PPS GSF ++YLPIL+PTIRFL DNNSP L+N+YPYFS
Sbjct: 153 GLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFS 212

Query: 233 YINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 292
           Y+ TPNM LD+A+FTG +S+V D +  YQNLFDAILD VYSAL+K+ GG LE+VVSETGW
Sbjct: 213 YVATPNMELDYAIFTG-TSLVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSETGW 272

Query: 293 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 352
           PTEGG+AATVDNA  YNNNLIQHVK GTPKR GR IETYVFAMFDENEK TPPEVE+HWG
Sbjct: 273 PTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 353 LFSPNKQIK 361
           LFSPNKQ K
Sbjct: 333 LFSPNKQPK 340

BLAST of Lcy03g006790 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.6e-136
Identity = 246/316 (77.85%), Postives = 275/316 (87.03%), Query Frame = 0

Query: 52  VAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPND 111
           VAQIGVCYGQ GNDLPSP EVIDLYNQNNIQRMRLY PNQ TF+ALRGS IELML LPND
Sbjct: 32  VAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQATFNALRGSSIELMLGLPND 91

Query: 112 QIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISA 171
           +IQ+MAA+QDNAN+WVQDNILN  D+ FKY+VVGNEIKTN+EAA FLVP M+NIQNAISA
Sbjct: 92  EIQSMAATQDNANAWVQDNILNFPDVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISA 151

Query: 172 MGLRDQIKVSTAFDTGIL-LESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYF 231
           +GL  +IKVSTAF TGIL +ES PPS GSF + YLPIL+PTIRFL DNNSP LVN+YPYF
Sbjct: 152 VGLGGKIKVSTAFHTGILSVESFPPSHGSFDASYLPILNPTIRFLLDNNSPLLVNLYPYF 211

Query: 232 SYINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETG 291
           SY+ TP+M LD+A+FTG +S+VVD +  YQNLF AILD VYSAL+K+ GG LEIVVSETG
Sbjct: 212 SYVGTPSMELDYAIFTG-TSLVVDGEFNYQNLFHAILDTVYSALEKNGGGSLEIVVSETG 271

Query: 292 WPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHW 351
           WPT+GG+AATVDNA  Y NNLIQ VK GTPKR GR IETYVFAMFDENEK TPPEVE+HW
Sbjct: 272 WPTDGGEAATVDNARTYTNNLIQRVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHW 331

Query: 352 GLFSPNKQIKYQVNFN 367
           GLFSPNKQ K+ V+FN
Sbjct: 332 GLFSPNKQPKFSVSFN 346

BLAST of Lcy03g006790 vs. ExPASy TrEMBL
Match: A0A6J1JIH6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 6.1e-136
Identity = 239/323 (73.99%), Postives = 278/323 (86.07%), Query Frame = 0

Query: 46  FVQFHVV-AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIEL 105
           F + H+  AQIGVCYGQ GNDLPSP EVI+LY QNNIQRMRLY P+ PTFDALRGS IEL
Sbjct: 23  FARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIEL 82

Query: 106 MLDLPNDQIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRN 165
           ML +PN++++ +A  Q+ A+SWV DN+++ GD+KFKY+VVGNEIKTND+AASFLVP M+N
Sbjct: 83  MLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQN 142

Query: 166 IQNAISAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLV 225
           IQNAISA+GL  QIKVSTAFDTGIL ES PPSKGSF++DYLPIL+PTIRFL DNN+P LV
Sbjct: 143 IQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLV 202

Query: 226 NVYPYFSYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLE 285
           NVYPYFSY+ NT ++++D+ALF G S  V D Q  Y NLFDAILDAVYSAL+K+   GLE
Sbjct: 203 NVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLE 262

Query: 286 IVVSETGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTP 345
           I VSETGWPTEGG+AATV+NA+ YNNNLIQHVK GTPKR G+P+ETYVFAMFDENEK TP
Sbjct: 263 IAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTP 322

Query: 346 PEVEKHWGLFSPNKQIKYQVNFN 367
           PEVEKHWGLFSP+K  KY+VNFN
Sbjct: 323 PEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Lcy03g006790 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 240/323 (74.30%), Postives = 277/323 (85.76%), Query Frame = 0

Query: 46  FVQFHVV-AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIEL 105
           F   H+  AQIGVCYGQ GNDLPSP EVI+LY QNNIQRMRLY  N PTFDALRGS IEL
Sbjct: 23  FASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDALRGSNIEL 82

Query: 106 MLDLPNDQIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRN 165
           ML +PN++++ +A  Q+ A+SWV DN+++ GD+KFKY+VVGNEIKT D+AASFLVP M+N
Sbjct: 83  MLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQN 142

Query: 166 IQNAISAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLV 225
           IQNAISA+GL  QIKVSTAFDTGIL ES PPSKGSF++DYLPIL+PTIRFL DNN+P LV
Sbjct: 143 IQNAISAVGLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLV 202

Query: 226 NVYPYFSYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLE 285
           NVYPYFSY+ NT ++++D+ALF G S  V D Q  Y NLFDAILDAVYSAL+K+ G GLE
Sbjct: 203 NVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEKAGGDGLE 262

Query: 286 IVVSETGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTP 345
           IVVSETGWPTEGG+AATV+NA+ YNNNLIQHVK GTPKR G+P+ETYVFAMFDENEK TP
Sbjct: 263 IVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTP 322

Query: 346 PEVEKHWGLFSPNKQIKYQVNFN 367
           PEVEKHWGLFSP+K  KY+VNFN
Sbjct: 323 PEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Lcy03g006790 vs. NCBI nr
Match: XP_011659353.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE8653333.1 hypothetical protein Csa_023295 [Cucumis sativus])

HSP 1 Score: 507.7 bits (1306), Expect = 8.5e-140
Identity = 250/315 (79.37%), Postives = 277/315 (87.94%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQIGVCYGQ GNDLPSP EVIDLYNQNNIQRMRLY PNQ TF+ALRGS IELML LPNDQ
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPNDQ 92

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 172
           IQ+MAA+QDNAN+W+QDNILN  D+ FKY+VVGNEIKTN+EAA FLVP M+NIQNAISA+
Sbjct: 93  IQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 173 GLRDQIKVSTAFDTGIL-LESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFS 232
           GL+ QIKVSTAF TGIL  ES PPS GSF ++YLPIL+PTIRFL DNNSP L+N+YPYFS
Sbjct: 153 GLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFS 212

Query: 233 YINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 292
           Y+ TPNM LD+A+FTG +S+V D +  YQNLFDAILD VYSAL+K+ GG LE+VVSETGW
Sbjct: 213 YVATPNMELDYAIFTG-TSLVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSETGW 272

Query: 293 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 352
           PTEGG+AATVDNA  YNNNLIQHVK GTPKR GR IETYVFAMFDENEK TPPEVE+HWG
Sbjct: 273 PTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 353 LFSPNKQIKYQVNFN 367
           LFSPNKQ KY VNFN
Sbjct: 333 LFSPNKQPKYPVNFN 346

BLAST of Lcy03g006790 vs. NCBI nr
Match: XP_038895085.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 505.0 bits (1299), Expect = 5.5e-139
Identity = 245/314 (78.03%), Postives = 278/314 (88.54%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           A+IGVCYGQ GNDLPSP EVI+LY QNNIQRMRLY PNQPTFDALRGS IELML +PNDQ
Sbjct: 33  AEIGVCYGQLGNDLPSPQEVIELYKQNNIQRMRLYAPNQPTFDALRGSNIELMLGIPNDQ 92

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 172
           I++MAA+QDNAN+WVQ+NILN  D+KFKY+VVGNEIKTN+EAA+FLVP M+NIQNAISA+
Sbjct: 93  IRSMAATQDNANAWVQENILNFPDVKFKYIVVGNEIKTNEEAATFLVPAMQNIQNAISAV 152

Query: 173 GLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSY 232
           GL+ QIKVSTAFDTGIL +S PPS+GSF ++YLPIL+PT RFL DN+SP LVN+YPYFSY
Sbjct: 153 GLQGQIKVSTAFDTGILSQSFPPSQGSFDTNYLPILNPTFRFLLDNSSPLLVNLYPYFSY 212

Query: 233 INTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGWP 292
           + TP M LD+A+FTG +S++ D +L YQNLFDAILDAVYSAL+KS GG LEIVVSETGWP
Sbjct: 213 VTTPRMGLDYAIFTG-TSLIGDGELNYQNLFDAILDAVYSALEKSGGGSLEIVVSETGWP 272

Query: 293 TEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWGL 352
           T+ G AATVDNA  YNNNLIQHV  GTPKR GR IETY+FAMFDENEK TPPEVE+HWGL
Sbjct: 273 TDHGDAATVDNARTYNNNLIQHVNQGTPKRRGRAIETYLFAMFDENEKTTPPEVERHWGL 332

Query: 353 FSPNKQIKYQVNFN 367
           FSPNKQ KY VNFN
Sbjct: 333 FSPNKQPKYSVNFN 345

BLAST of Lcy03g006790 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 497.3 bits (1279), Expect = 1.1e-136
Identity = 245/315 (77.78%), Postives = 277/315 (87.94%), Query Frame = 0

Query: 53  AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQ 112
           AQIGVCYGQ G+DLPSP EVIDLYNQNNI+RMRLY PNQP+ +ALRGS IELML +PN +
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 113 IQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 172
           ++AMA +Q+NANSW QDN+L+ GD+KFKY+VVGNEIK +D AA FLVP M+NIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 173 GLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSY 232
           GLR +IKVSTA DTG+L ES PPSKGSFRSDYLPILDPTIRFL DNNSP L+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 233 I-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 292
           I NT +++LD+ALFTG  S+V D QL YQNLFDAILDAVYSAL+KS GGGLEIVVSE+GW
Sbjct: 212 ISNTKDISLDYALFTG-MSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 271

Query: 293 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 352
           PT+GG+AA VDNA  YNNNLIQHVK GTPKRPG PIETYVFAMFDEN+K TPPEVEKHWG
Sbjct: 272 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 331

Query: 353 LFSPNKQIKYQVNFN 367
           LFSPNKQ KY VNF+
Sbjct: 332 LFSPNKQPKYLVNFH 345

BLAST of Lcy03g006790 vs. NCBI nr
Match: XP_008459107.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 495.7 bits (1275), Expect = 3.3e-136
Identity = 246/316 (77.85%), Postives = 275/316 (87.03%), Query Frame = 0

Query: 52  VAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPND 111
           VAQIGVCYGQ GNDLPSP EVIDLYNQNNIQRMRLY PNQ TF+ALRGS IELML LPND
Sbjct: 32  VAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQATFNALRGSSIELMLGLPND 91

Query: 112 QIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISA 171
           +IQ+MAA+QDNAN+WVQDNILN  D+ FKY+VVGNEIKTN+EAA FLVP M+NIQNAISA
Sbjct: 92  EIQSMAATQDNANAWVQDNILNFPDVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISA 151

Query: 172 MGLRDQIKVSTAFDTGIL-LESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYF 231
           +GL  +IKVSTAF TGIL +ES PPS GSF + YLPIL+PTIRFL DNNSP LVN+YPYF
Sbjct: 152 VGLGGKIKVSTAFHTGILSVESFPPSHGSFDASYLPILNPTIRFLLDNNSPLLVNLYPYF 211

Query: 232 SYINTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETG 291
           SY+ TP+M LD+A+FTG +S+VVD +  YQNLF AILD VYSAL+K+ GG LEIVVSETG
Sbjct: 212 SYVGTPSMELDYAIFTG-TSLVVDGEFNYQNLFHAILDTVYSALEKNGGGSLEIVVSETG 271

Query: 292 WPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHW 351
           WPT+GG+AATVDNA  Y NNLIQ VK GTPKR GR IETYVFAMFDENEK TPPEVE+HW
Sbjct: 272 WPTDGGEAATVDNARTYTNNLIQRVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHW 331

Query: 352 GLFSPNKQIKYQVNFN 367
           GLFSPNKQ K+ V+FN
Sbjct: 332 GLFSPNKQPKFSVSFN 346

BLAST of Lcy03g006790 vs. NCBI nr
Match: XP_022987103.1 (probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima])

HSP 1 Score: 493.8 bits (1270), Expect = 1.3e-135
Identity = 239/323 (73.99%), Postives = 278/323 (86.07%), Query Frame = 0

Query: 46  FVQFHVV-AQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIEL 105
           F + H+  AQIGVCYGQ GNDLPSP EVI+LY QNNIQRMRLY P+ PTFDALRGS IEL
Sbjct: 23  FARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIEL 82

Query: 106 MLDLPNDQIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRN 165
           ML +PN++++ +A  Q+ A+SWV DN+++ GD+KFKY+VVGNEIKTND+AASFLVP M+N
Sbjct: 83  MLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQN 142

Query: 166 IQNAISAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLV 225
           IQNAISA+GL  QIKVSTAFDTGIL ES PPSKGSF++DYLPIL+PTIRFL DNN+P LV
Sbjct: 143 IQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLV 202

Query: 226 NVYPYFSYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLE 285
           NVYPYFSY+ NT ++++D+ALF G S  V D Q  Y NLFDAILDAVYSAL+K+   GLE
Sbjct: 203 NVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLE 262

Query: 286 IVVSETGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTP 345
           I VSETGWPTEGG+AATV+NA+ YNNNLIQHVK GTPKR G+P+ETYVFAMFDENEK TP
Sbjct: 263 IAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTP 322

Query: 346 PEVEKHWGLFSPNKQIKYQVNFN 367
           PEVEKHWGLFSP+K  KY+VNFN
Sbjct: 323 PEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Lcy03g006790 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 389.8 bits (1000), Expect = 2.4e-108
Identity = 187/314 (59.55%), Postives = 237/314 (75.48%), Query Frame = 0

Query: 54  QIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQI 113
           Q+GVCYG+NGN+LPSP E I L+ Q NIQR+RLY+P+     ALRGS IE+ L LPN  +
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 114 QAMAASQDNANSWVQDNILNHGD-IKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAM 173
           Q++A+SQ  AN+WVQ  ++N+ + ++F+Y+ VGNE+K +D  A FLVP M NI  A+ A 
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 174 GLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSY 233
           GL  +IKVST+ D G+L ES PPSKGSFR D + +++P IRFL   NSP L+N+Y YFSY
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 234 I-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGW 293
             N   + LD+ALFT PS +V D   +YQNLFDA+LDA+YSAL+KS G  LEIVV+ETGW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 294 PTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKHWG 353
           PT GG    ++NA  YNNNLI+HVK GTPKRPG+ IETY+FA++DEN+K TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 354 LFSPNKQIKYQVNF 366
           LF PNKQ KY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

BLAST of Lcy03g006790 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 358.6 bits (919), Expect = 5.9e-99
Identity = 183/317 (57.73%), Postives = 233/317 (73.50%), Query Frame = 0

Query: 52  VAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPND 111
           V QIGVCYG+NGN+L    EV+ LY Q NI+RMRLY PNQ T +ALRGS IEL+LD+PN 
Sbjct: 32  VGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNP 91

Query: 112 QIQAMAASQDNANSWVQDNILNHGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISA 171
            +Q +A+SQ  A++WV++N+ N+ ++ F+Y+ VGNE++ +D+AASF++P M+NI+ A+S+
Sbjct: 92  DLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSS 151

Query: 172 MGLRDQIKVSTAFDT-GILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYF 231
           +G    IKVSTA DT GI     PPS G+F  ++   + P I FL+   SP LVN YPYF
Sbjct: 152 LG----IKVSTAIDTRGI--SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYF 211

Query: 232 SYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSET 291
           SY  N  ++ LD+ LFT PS+VV D Q  Y+NLF AILD VY++L+K+ GG LEIVVSE+
Sbjct: 212 SYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSES 271

Query: 292 GWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVEKH 351
           GWPT GG A  VDNA  Y NNLIQ VK G+P+RPGR  ETY+FAMFDEN K+  PE EK 
Sbjct: 272 GWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQ-GPETEKF 331

Query: 352 WGLFSPNKQIKYQVNFN 367
           WGLF PN Q KY VNFN
Sbjct: 332 WGLFLPNLQPKYVVNFN 341

BLAST of Lcy03g006790 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 356.3 bits (913), Expect = 2.9e-98
Identity = 179/319 (56.11%), Postives = 233/319 (73.04%), Query Frame = 0

Query: 50  HVVAQIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLP 109
           H   QIGVCYG  G+ LPSP +V+ LY Q NIQRMRLY P+     ALRGS IEL+LD+P
Sbjct: 27  HTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVP 86

Query: 110 NDQIQAMAASQDNANSWVQDNILNHGD-IKFKYVVVGNEIKTNDEAASFLVPTMRNIQNA 169
           +  ++ +A+SQ  A+ WVQ+N+ ++ D ++F+Y+ VGNE+K +     FL+  M+NI+NA
Sbjct: 87  SSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENA 146

Query: 170 ISAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYP 229
           +S  GL  ++KVSTA  T    ++SPPS+G FR +Y   L+P I FL    SP LVN+YP
Sbjct: 147 VSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYP 206

Query: 230 YFSYI-NTPNMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVS 289
           YFSY+ +T N+ LD+ALFT  S+V  D   +YQNLFDA LD+VY+AL+KS GG LEIVVS
Sbjct: 207 YFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVS 266

Query: 290 ETGWPTEGGQAATVDNASAYNNNLIQHVKVGTPKRPGRPIETYVFAMFDENEKKTPPEVE 349
           ETGWPTEG    +V+NA  Y NNLIQHVK G+P+RPG+ IETY+FAMFDEN+K+  P  E
Sbjct: 267 ETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKKE--PTYE 326

Query: 350 KHWGLFSPNKQIKYQVNFN 367
           K WGLF P++Q KY+VNFN
Sbjct: 327 KFWGLFHPDRQSKYEVNFN 339

BLAST of Lcy03g006790 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-87
Identity = 159/316 (50.32%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 55  IGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQIQ 114
           +GVCYG  GN+LPS  + I L+ QNNI+R+RLY PNQ   +ALR + IE+++ +PN  ++
Sbjct: 24  VGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVPNTDLR 83

Query: 115 AMAASQDNANSWVQDNILN-HGDIKFKYVVVGNEIKTNDEAASFLVPTMRNIQNAISAMG 174
           ++  +  +A SW+Q+N+LN +  + FKY+ VGNE+  ++     ++P MRN+ +A+    
Sbjct: 84  SL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSN-GGDVVLPAMRNVYDALRGAN 143

Query: 175 LRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPYFSYI 234
           L+D+IKVSTA D  ++  S PPS G FR D    +DP I FL   NS  L N+YPYFSY+
Sbjct: 144 LQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYFSYV 203

Query: 235 NTP-NMALDFALFTGPSSVVVDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVSETGWP 294
           + P +++L +ALFT PS VV D    YQNLFDA+LD VYSA+++S GG L +VVSE+GWP
Sbjct: 204 DNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSESGWP 263

Query: 295 TEGGQAATVDNASAYNNNLIQHVKV--GTPKRPGRPIETYVFAMFDENEKKTPPEVEKHW 354
           + GG AA+ DNA A+  NL   V+   GTPKRPGR +ETY+FAMFDEN+K   PE+EK++
Sbjct: 264 SNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKS--PEIEKNF 323

Query: 355 GLFSPNKQIKYQVNFN 367
           GLF PNKQ K+ + F+
Sbjct: 324 GLFFPNKQPKFPITFS 335

BLAST of Lcy03g006790 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.7e-69
Identity = 135/324 (41.67%), Postives = 212/324 (65.43%), Query Frame = 0

Query: 54  QIGVCYGQNGNDLPSPPEVIDLYNQNNIQRMRLYTPNQPTFDALRGSKIELMLDLPNDQI 113
           ++GVCYG++ +DLP+P +V+ L  Q+NI+ +R+Y  N     A   + IELM+ +PN  +
Sbjct: 25  KVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDL 84

Query: 114 QAMAASQDNANSWVQDNIL-NHGDIKFKYVVVGNEIKTND---EAASFLVPTMRNIQNAI 173
            A + SQ N ++W+++++L  +   K  Y+ VG E  T+D    A+SF+VP M+N+  A+
Sbjct: 85  NAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE-STDDPHINASSFVVPAMQNVLTAL 144

Query: 174 SAMGLRDQIKVSTAFDTGILLESSPPSKGSFRSDYLPILDPTIRFLNDNNSPFLVNVYPY 233
             +GL  +IKVST    GIL  S PPS G+F S Y   L P + FL +N SPF++++YPY
Sbjct: 145 RKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPY 204

Query: 234 FSYINTP-NMALDFALFTGPSSVV-VDDQLAYQNLFDAILDAVYSALDKSNGGGLEIVVS 293
           ++Y ++P N++LD+ LF   S V+  +  L Y+N+FDA +DA+Y AL   N   ++I+V+
Sbjct: 205 YAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVT 264

Query: 294 ETGWPTEGG----QAATVDNASAYNNNLIQHV--KVGTPKRPGRPIETYVFAMFDENEKK 353
           ETGWPT+G      AA+ DNA  YN+N+I+HV    GTP +PG  +  Y+F++F+EN +K
Sbjct: 265 ETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNEN-RK 324

Query: 354 TPPEVEKHWGLFSPNKQIKYQVNF 366
              + E++WGLF P++   YQ++F
Sbjct: 325 AGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524089.5e-11061.01Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
Q037731.5e-10761.64Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1[more]
Q9M2M03.4e-10759.55Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN... [more]
P364011.9e-10257.23Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4... [more]
F4J2708.3e-9857.73Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1CBL75.6e-13777.78(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ... [more]
A0A0A0LYQ87.2e-13779.29(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
A0A1S3C9Z31.6e-13677.85(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV... [more]
A0A6J1JIH66.1e-13673.99(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=... [more]
A0A6J1HCY31.0e-13574.30(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P... [more]
Match NameE-valueIdentityDescription
XP_011659353.18.5e-14079.37glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE86533... [more]
XP_038895085.15.5e-13978.03glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida][more]
XP_022139185.11.1e-13677.78glucan endo-1,3-beta-glucosidase-like [Momordica charantia][more]
XP_008459107.13.3e-13677.85PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo][more]
XP_022987103.11.3e-13573.99probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G57270.12.4e-10859.55beta-1,3-glucanase 1 [more]
AT3G57240.15.9e-9957.73beta-1,3-glucanase 3 [more]
AT3G57260.12.9e-9856.11beta-1,3-glucanase 2 [more]
AT4G16260.11.8e-8750.32Glycosyl hydrolase superfamily protein [more]
AT5G56590.13.7e-6941.67O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 55..365
e-value: 2.4E-117
score: 392.0
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 282..295
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 53..366
e-value: 2.5E-131
score: 439.4
NoneNo IPR availablePANTHERPTHR32227:SF138GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATEDcoord: 49..363
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 49..363
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 55..364

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g006790.1Lcy03g006790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds