Lcy03g006730 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g006730
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase
LocationChr03: 35903298 .. 35905362 (+)
RNA-Seq ExpressionLcy03g006730
SyntenyLcy03g006730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGGAAGAAAAACGCAGCCGCCGGTCACTGGGTTTCGTTGGTCTCGGTTCAGAGGTCCGTTCGGCGCCGGTTGTGGGTCTCGGGTTGTACGGCAGAAGTGGGTCTCGATTAGAGACGTCGTGGGTCCCTGTTCAGGCGCCGGTGTGGGCCTCGGAGAAGCGACTTCGCGTGTGTTTCGGTTGAGTGCAGCTGTGGGTCTCGGTTTAGGGACTGTTTTGCTCCGGTTTGAAGCTCGTTTCTTCTCAGTTGTGGGTCTCGGTATAGGAGGCTGAATCGTGTTGTGGGTGTTTCGTTGGAAGCTCAAATCGGCCAAGATTTGAGGTATTTCACTGGTTGAATTTAATACCATTTATACTCTGTATTTGTGACTTGATGCTCGTTGATAGAAGTCTCATATAAGCTGGTTCTCAATCTGGAGGTATATCGAAAGGTTAAATCACTGTATATCTTTGTGTTCTTTCTCTCTTGGTTGTGCTTATTTGTTGCTGCTCTTTTCCAATGGAAAATTTGTAGAACTGTCCAATTAGATTCTTTGAGCGAGAATTGAGTGTGTAGAGCGATTGACAACTGAAGTTTTTAAAAGACATTTATTCCAGATAGTAGTTTCGATTTTAACTACTTATTGTCCAAAATGTGTTGCTGTTATTGTGTATCTTTGTTTTATTTATTTGTTTTTTCCCCTAAATTTCTACAGAAGTTGGAATTCTTGATTCTTTATAAACTTTTGAAGACCAAGGATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGGTAGGATCGTAGATCTAATGTGTGTTTTCAAGTTACCCTGCTGAGTAATTGTTCTATAATGGTTTTCCATTTTTAAACAGATCTTGAGCCTGTTTAAGTAGTACTTATTGCTTGTTTCCCTCTTCTTTCAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGGTTCTAAATGACCCTATTGTCATTTAACTTCATTGCTGAGATATGTTGAATTGTAGTGTTCACTTTTGGTCCTCTTTTGTGTAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAACACTAATATAGTTTATGAATTCTTTAACAAGGAAATTTGAAGTTTTGTATTCTTTTTCATCACTCTGGAACCTTGATTCTTATTTAAATTATTGAATGTTCAATAATTTTATTCCTGATATCATCACTCTTCATCATCTTGGCTAATTTCATGAGTTTTGTACTTTTGACTTTGCCAG

mRNA sequence

GAGAGGAAGAAAAACGCAGCCGCCGGTCACTGGGTTTCGTTGGTCTCGGTTCAGAGGTCCGTTCGGCGCCGGTTGTGGGTCTCGGGTTGTACGGCAGAAGTGGGTCTCGATTAGAGACGTCGTGGGTCCCTGTTCAGGCGCCGGTGTGGGCCTCGGAGAAGCGACTTCGCGTGTGTTTCGGTTGAGTGCAGCTGTGGGTCTCGGTTTAGGGACTGTTTTGCTCCGGTTTGAAGCTCGTTTCTTCTCAGTTGTGGGTCTCGGTATAGGAGGCTGAATCGTGTTGTGGGTGTTTCGTTGGAAGCTCAAATCGGCCAAGATTTGAGAAGTTGGAATTCTTGATTCTTTATAAACTTTTGAAGACCAAGGATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAACACTAATATAGTTTATGAATTCTTTAACAAGGAAATTTGAAGTTTTGTATTCTTTTTCATCACTCTGGAACCTTGATTCTTATTTAAATTATTGAATGTTCAATAATTTTATTCCTGATATCATCACTCTTCATCATCTTGGCTAATTTCATGAGTTTTGTACTTTTGACTTTGCCAG

Coding sequence (CDS)

ATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAA

Protein sequence

MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFARLRCIS
Homology
[Close]
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.5e-65
Identity = 140/298 (46.98%), Postives = 189/298 (63.42%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 189
           +VAPTGH+HT +FH+QRA  ARE+F++  L+  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        +V +P       + E N M  D+S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLETN-MGSDASPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.0e-65
Identity = 139/298 (46.64%), Postives = 186/298 (62.42%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH+HT +FH+QRA  ARE+F++  +   VTVR +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 250 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        +V +PP    L +        D+S      P  EA GHTGYLTFA
Sbjct: 244 -------------TVSLPP--PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.5e-63
Identity = 137/301 (45.51%), Postives = 187/301 (62.13%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 189
           ++APTGH+HT +FH+QRA  ARE+F++  ++  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGK---PESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTF 305
                 P+    D  G+VG                  D++      P  E  GHTGYLTF
Sbjct: 244 TVSLPLPDLGADDLEGNVG-----------------SDATPFRSGTPMKETVGHTGYLTF 285

[Close]
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 9.0e-61
Identity = 135/298 (45.30%), Postives = 181/298 (60.74%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH+HT +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRS-KFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 250 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        S+ +P    R           D++      P  E  GHTGYLTFA
Sbjct: 244 T-----------VSLPVPDLGAR--------PGPDAAPFRSGTPMKETVGHTGYLTFA 280

[Close]
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 9.0e-61
Identity = 129/306 (42.16%), Postives = 190/306 (62.09%), Query Frame = 0 

Query: 16  IRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFGSKVLSNKG-GFVYLL 75
           I++GD V++Y   D M  + +  ++ + ++FG ++H + IGK +GSK+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 76  APTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTG 135
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 136 HVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQG-QGFPEEFVGL-ADSVFLDLPQP 195
           H+ TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 196 WLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLR-SKFTDIRTFEVLLRTYEVREGKPE 255
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT++ R  + E
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 256 SSQVDGGGSVGIPPCKRRLRSNEANDMNDD------------SSTTVMVRPCSEARGHTG 306
              +        P       +N  N++ ++                ++ +P +EARGHTG
Sbjct: 248 ELNLTN------PYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEARGHTG 307

[Close]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match: A0A6J1JHY4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.1e-165
Identity = 291/310 (93.87%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match: A0A6J1DWC1 (tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024670 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.0e-164
Identity = 289/310 (93.23%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+SKKISFTRSI DGDLVIVYERHDIMKAVKVCE ST QNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLT+LVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVRE K E SQVDGGGSVG+PPCKRRLRSN+ANDM++D STTVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTF+RLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.0e-164
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCE+STFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match: A0A6J1J9F9 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.6e-164
Identity = 290/309 (93.85%), Postives = 301/309 (97.41%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCI 310
           LTFARLRC+
Sbjct: 301 LTFARLRCL 308

[Close]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.5e-164
Identity = 287/310 (92.58%), Postives = 301/310 (97.10%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTDE K ISFTRSIRDGDLVIVYERHDIMKAVKVC+ ST QNRFGVFKHSDWIGKPFG
Sbjct: 396 MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 455

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG
Sbjct: 456 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 515

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLTSLVTV VRDIQGQGFPEEFVG
Sbjct: 516 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 575

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLL
Sbjct: 576 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 635

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEV+EG+ ES+QVDGGGSVG+PPCKRRLRS E++DM+++SSTTVMVRPCSEARGHTGY
Sbjct: 636 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 695

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 696 LTFARLRCLS 705

[Close]
BLAST of Lcy03g006730 vs. NCBI nr
Match: XP_038894928.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894929.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894930.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894931.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894932.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894933.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894934.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894935.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894936.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894937.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida])

HSP 1 Score: 591.7 bits (1524), Expect = 3.8e-165
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTDE KKISFTRSIRDGDLVIVYERHDIMKAVKVCE ST QNRFGVFKH+DWIGKPFG
Sbjct: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLTSLVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEV+EG+ ES+QVDGGGSV +PPCKRRLRS E++DMNDDSST VMVRPCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 310

[Close]
BLAST of Lcy03g006730 vs. NCBI nr
Match: XP_022987109.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 1.9e-164
Identity = 291/310 (93.87%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Lcy03g006730 vs. NCBI nr
Match: KAG7023747.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCE+STFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAESMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EANDMN DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDMNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Lcy03g006730 vs. NCBI nr
Match: XP_022158112.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia] >XP_022158113.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 289/310 (93.23%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+SKKISFTRSI DGDLVIVYERHDIMKAVKVCE ST QNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLT+LVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVRE K E SQVDGGGSVG+PPCKRRLRSN+ANDM++D STTVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTF+RLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of Lcy03g006730 vs. NCBI nr
Match: XP_022961161.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucurbita moschata] >XP_022961162.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucurbita moschata])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCE+STFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLRCIS 311
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Lcy03g006730 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.0e-136
Identity = 236/318 (74.21%), Postives = 271/318 (85.22%), Query Frame = 0 

Query: 1   MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 60
           MLPT+  K  SF R I DGDLVIVYERHD+MK VKV +    QNRFGV+KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120
           +KV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LE+VPGC+VLESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 180
           SL+TSLARAVAPTGHV++FDFHEQRA SAREDFEKTG++SLVTV VRDIQG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLA+PSA +MLKQDGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 241 RTYEVREGKPESSQVDGGG----SVGIPPCKRRLRSNE----ANDMNDDSSTTVMVRPCS 300
           RTYEV+E K ++S + G      + G+ PCKR+ RSNE    + D + ++S+ VM RPCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 301 EARGHTGYLTFARLRCIS 311
           EARGHTGYLTFARL+C++
Sbjct: 301 EARGHTGYLTFARLQCLA 318

The following BLAST results are available for this feature:
BLAST of Lcy03g006730 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q80XC23.5e-6546.98tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
Q96FX76.0e-6546.64tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
Q6AY462.5e-6345.51tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
A6H7919.0e-6145.30tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
Q86JJ09.0e-6142.16tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
BLAST of Lcy03g006730 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JHY49.1e-16593.87tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1DWC12.0e-16493.23tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1H9F42.0e-16493.55tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J9F92.6e-16493.85tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0LYQ84.5e-16492.58(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
BLAST of Lcy03g006730 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038894928.13.8e-16593.55tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa h... [more]
XP_022987109.11.9e-16493.87tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform... [more]
KAG7023747.14.2e-16493.55tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cu... [more]
XP_022158112.14.2e-16493.23tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica c... [more]
XP_022961161.14.2e-16493.55tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [... [more]
BLAST of Lcy03g006730 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14600.13.0e-13674.21S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PIRSFPIRSF017269GCD14coord: 10..309
e-value: 2.9E-98
score: 325.8
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 69..305
e-value: 2.1E-97
score: 325.7
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PANTHERPTHR12133TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASEcoord: 1..307
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROSITEPS51620SAM_TRM61coord: 19..307
score: 99.860229
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..308
e-value: 3.1E-81
score: 274.1
NoneNo IPR availableGENE3D3.10.330.20coord: 10..77
e-value: 8.0E-26
score: 91.9
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 47..248
e-value: 1.3E-40
score: 136.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..284
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 1..307
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 112..209
e-value: 0.00452634
score: 34.3279
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 15..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g006730.1Lcy03g006730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity