Lcy03g005440 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g005440 vs. ExPASy Swiss-Prot
Match: Q9LKR8 (Rubisco accumulation factor 1.1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.1 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.2e-124
Identity = 256/454 (56.39%), Postives = 327/454 (72.03%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSPRLFLRRRPTPISAILNPSPSPSPSPLAAKP 60
           MLS T      T+LS++    + T  F + R   R+  T I++ L P+ +    P   K 
Sbjct: 1   MLSLTA-----TTLSSSIFTQSKTHGFFNTRPVYRKPFTTITSALIPASNRQAPP---KQ 60

Query: 61  QVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEIT 120
           Q+YQPFRPPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+T
Sbjct: 61  QLYQPFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELT 120

Query: 121 GISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKY 180
           GISGV+QN LIVGAQVR SLVQ+   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+Y
Sbjct: 121 GISGVEQNSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEY 180

Query: 181 IVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEAR 240
           IVD+  D KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E R
Sbjct: 181 IVDHGFDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIR 240

Query: 241 TAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEATTV 300
           T  LE AL+  VTE A+  ++REL GE E +E  +E+    +RVPVVRL+ GEVA A++V
Sbjct: 241 TTLLEQALETAVTEKAKKAVLRELHGESE-EERVKEEEIKIIRVPVVRLRFGEVAGASSV 300

Query: 301 MVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVA 360
           +V+PVCKAEEGE+ +  APME  S GEFGVV AE+ W RWVVLP W+P+  + R GV V+
Sbjct: 301 VVLPVCKAEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAV-RKGVAVS 360

Query: 361 FNDAR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNAL 420
           F+D R  LPW      K EAI+VV DR +  V A++G+Y         +G +K+ RG  L
Sbjct: 361 FSDDREVLPWN----GKGEAIMVVIDREKKTVEADNGYYYLVV----ADGGMKLDRGLVL 420

Query: 421 MEKGVRESLGTVLLVVRPPKDMEDD-QLSDEDWD 453
            EKGV ESLG V+LVVRPP+D +D+ Q++DEDWD
Sbjct: 421 KEKGVNESLGMVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of Lcy03g005440 vs. ExPASy Swiss-Prot
Match: Q9SR19 (Rubisco accumulation factor 1.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.2 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 9.3e-123
Identity = 231/406 (56.90%), Postives = 302/406 (74.38%), Query Frame = 0

Query: 53  PSPLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFT 112
           P    A+ Q+YQPFRPP SP+P Q+RSLD+ GK+++L+ R+ LWFEYAPLISSL  +GFT
Sbjct: 55  PKNPPARQQLYQPFRPPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAPLISSLYTDGFT 114

Query: 113 PPTIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAE 172
           PPTIEE+TGIS ++QN LIVGAQVR S++Q+I   +P+LI+AFDT GAELLYEIRLLS  
Sbjct: 115 PPTIEELTGISSIEQNRLIVGAQVRDSILQSI--HEPELISAFDTGGAELLYEIRLLSTT 174

Query: 173 KRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSRE 232
           +R +AA +I+D  +D KGAQDLARA+KD+P RRGD GW  FDY+ PGDCL+F+YYR SRE
Sbjct: 175 QRVAAATFIIDRNIDSKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCLSFLYYRQSRE 234

Query: 233 YKNQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTE----EDNEAGVRVPVVR 292
            KN  + RT+ L  AL V  +E A++ +  EL G+ E ++  E    E+    +R+PVVR
Sbjct: 235 NKNPSDQRTSMLLQALGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEEVKAIRIPVVR 294

Query: 293 LKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEP 352
           LK GEVAEAT+V+V+PVCKAEEGEK +  APME+ + G+F VV AE+GW+RWVVLP+W P
Sbjct: 295 LKFGEVAEATSVVVLPVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWKRWVVLPSWNP 354

Query: 353 LRGLGRGGVVVAFNDAR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGG 412
           +  +G+GGV V+F D R  LPW      KEE +LVV DR RN V A+DG+YL  A     
Sbjct: 355 VAAIGKGGVAVSFRDDRKVLPWD----GKEEPLLVVADRVRNVVEADDGYYLVVA----- 414

Query: 413 EGVLKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDD-QLSDEDWD 453
           E  LK+++G+ L  + V+ESLG V+LVVRPP++ +DD Q S ++WD
Sbjct: 415 ENGLKLEKGSDLKAREVKESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of Lcy03g005440 vs. ExPASy Swiss-Prot
Match: Q7XDY9 (Rubisco accumulation factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RAF1 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.8e-95
Identity = 217/477 (45.49%), Postives = 286/477 (59.96%), Query Frame = 0

Query: 2   LSATTPATSLTSLSAAAAHTATTFLFHSPRLFLRRR---PTPISAILNPSPSP------- 61
           LS   P  + T+ +AAA H        +P    RRR    T  + IL P   P       
Sbjct: 4   LSHPHPHPASTTAAAAARHHHRRNAPFAPHHRRRRRFAHLTTSAVILGPDGRPIGGGPRD 63

Query: 62  -----SPSPLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSL 121
                +P P A   Q+YQPF PPPSPLP +Y+ LD   +L VL +RLGLW EYAPLIS+L
Sbjct: 64  NKLPFTPPPTAPPDQLYQPFHPPPSPLPDKYKDLDLGQRLAVLRDRLGLWHEYAPLISAL 123

Query: 122 IQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDT-SGAELLYE 181
            +EGFTP +IEE TGISGV+QN ++V  QVR SLV        +L+  FD+  G ELLYE
Sbjct: 124 SREGFTPSSIEEATGISGVEQNSVVVATQVRDSLVADEGGFPAELLRYFDSYGGPELLYE 183

Query: 182 IRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFM 241
           +R L+A +RA AA++ +D RL+ +G ++LAR+MKDFP+RRGD GWE+F  D PGDCLAF 
Sbjct: 184 LRFLNARQRADAARHAIDRRLEPRGVRELARSMKDFPQRRGDDGWEAFTRDNPGDCLAFA 243

Query: 242 YYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGV--- 301
            +R SRE     E   A LE AL+VV TE AR+ +  EL+       G E D+E G    
Sbjct: 244 RFRQSRE-AIDAEDSVAELERALEVVDTEPARARVEAELDRARRKAAGEEVDDEDGAANA 303

Query: 302 -------RVPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEE 361
                   VPVVRL  GEVAEATTV+++PV +  +G + +  AP   R++ + G+V  ++
Sbjct: 304 AAAASRPAVPVVRLMYGEVAEATTVLLLPVVREGDGGEALAHAPRRTRTDADLGMVEVDK 363

Query: 362 GWRRWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAED 421
           GW RW V+P W P+  +    VV+   D R LPWR     + E +LVV +R R EVV ED
Sbjct: 364 GWTRWAVVPGWGPVAEVAGEAVVIELADGRTLPWRS---AEAERVLVVANRGRREVV-ED 423

Query: 422 GFYLTAATVEGGEGVLKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           G Y+        EG L V++G  L  +GV E+   VL+VVRPP+D EDD +SD++WD
Sbjct: 424 GIYVVER-----EGRLVVEKGRKLAAEGVGEAAAEVLVVVRPPRD-EDDMISDDEWD 469

BLAST of Lcy03g005440 vs. ExPASy Swiss-Prot
Match: B4FR29 (Rubisco accumulation factor 1, chloroplastic OS=Zea mays OX=4577 GN=RAF1 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.2e-92
Identity = 216/480 (45.00%), Postives = 294/480 (61.25%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSPRLFLRRRPTPI--SAILNPSPSP------- 60
           MLS + P     + + A  H  T  ++H      RRR + I  SAIL P           
Sbjct: 1   MLSLSHPHPHPAASTTAPRHQRTAPVWH------RRRASHIAASAILLPGGGSTGGRGGP 60

Query: 61  -------SPSPLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLIS 120
                  +P P+A   Q+YQPF PPPSPLPP  R+LD + +L +L +R+GLW EYAPLIS
Sbjct: 61  GDRRLPFTPPPMAPPGQLYQPFHPPPSPLPPSLRNLDLSERLQILRDRMGLWHEYAPLIS 120

Query: 121 SLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVQTIDNS-DPDLIAAFDT-SGAEL 180
           SL ++GF P +I+E TGISGV+QN L+V +QVR SL+     +  PDL+  FD+  G E+
Sbjct: 121 SLSRDGFNPSSIQEATGISGVEQNCLVVASQVRDSLLDDRAAAFPPDLLPYFDSLGGPEV 180

Query: 181 LYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCL 240
           LYE+R L+A +RA AA++ +  RL+ KG ++LARAMK FPR RG++GWE+F  D P DCL
Sbjct: 181 LYELRFLNARQRADAARHAIGYRLEPKGVRELARAMKGFPRWRGEEGWEAFSKDSPADCL 240

Query: 241 AFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRELE-------GEGE-GKEGT 300
           AF  +R SRE  +  + R A LE AL+VV TE  R+ +  ELE       GE E  +EG 
Sbjct: 241 AFARFRQSREAIDVQD-RVAELERALQVVETESGRARVELELERARRKAAGEEEVDEEGE 300

Query: 301 EEDNEAGVR--VPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVV 360
           E+D  A +R  V VVRL+ GEVAEATTV+++PV +  +G   + +AP   +++   GVV 
Sbjct: 301 EDDAAASLRPGVTVVRLRYGEVAEATTVILLPVVRETDGVAAMESAPRRAKTDVGLGVVE 360

Query: 361 AEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVV 420
            +  W RW V+P W P+       VVV   D R LPWR++    EE +LV+ +RS+ EVV
Sbjct: 361 VDRAWARWAVVPGWGPVAEAADDAVVVELADGRRLPWRMS---DEEPVLVIANRSKKEVV 420

Query: 421 AEDGFYLTAATVEGGEGVLKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
            E+G Y+        EG L V+RG  L E+G+  +   V++VVRPPKD EDD +SDE+WD
Sbjct: 421 -EEGVYVLER-----EGRLVVERGKKLAEQGIAAAAAEVVIVVRPPKD-EDDMVSDEEWD 463

BLAST of Lcy03g005440 vs. ExPASy Swiss-Prot
Match: Q8YLP6 (RuBisCO accumulation factor 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=raf1 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.4e-22
Identity = 113/385 (29.35%), Postives = 181/385 (47.01%), Query Frame = 0

Query: 68  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 127
           PP +P P    +      L  L  + G W E+   I+SL + G+ P  I E TG   VQQ
Sbjct: 5   PPNAPNPENATNELAQELLRKLRQKQGNWVEWGQAIASLQKSGYNPQDIFEATGFEPVQQ 64

Query: 128 NILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 187
           N +IVG+QV  SL ++   +    +A + T G+++LYE+RLL+ E+RA+A      +++D
Sbjct: 65  NQVIVGSQVYNSLEKS--GASAATLAHYATRGSDVLYELRLLTHEERAAAGDLTFTHKVD 124

Query: 188 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 247
              A+++A+A+KDF R R     E F  + PGD +A+  ++L+R+Y +  E R+  +   
Sbjct: 125 ADEAREIAKAIKDFSRFRILP--EGFS-NHPGDAVAYQAWKLARQYSDLQE-RSRLIARG 184

Query: 248 LKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKI-GEVAEATTVMVMPVC 307
           L+   +E AR  I + L       + T          P  R     E+     V+     
Sbjct: 185 LRFAHSETARKQIEQLL------VDFTVVSQRPAPIPPFFRFDTEDELPRIVPVVGQLPL 244

Query: 308 KAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARA 367
           KAEE    + A P+    E E   +V   G + WV LP W+ L        ++A +D   
Sbjct: 245 KAEE----LKAVPLV--EEIEPFRLVKFSGEQAWVALPGWQVLLAAEDPVTILATSDR-- 304

Query: 368 LPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNALMEKGVRE 427
             +      +   +LVV DRS+ E    D  Y     V   +G L  Q      E  +  
Sbjct: 305 --FPKQNQTEPGPVLVVVDRSQRE--WNDFSYF----VVDHDGELDFQWFETKPEFPI-- 357

Query: 428 SLGTVLLVVRPPKDMEDDQLSDEDW 452
            LG V+++VRP + + D+ ++ + W
Sbjct: 365 -LGKVIILVRPRRIL-DENVTKDSW 357

BLAST of Lcy03g005440 vs. ExPASy TrEMBL
Match: A0A6J1F451 (rubisco accumulation factor 1.1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439956 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 6.3e-207
Identity = 381/454 (83.92%), Postives = 407/454 (89.65%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PA   TSLSAAAAHT TTFL H+P  RLFLRR P PISA LN  PSPSPSPL A
Sbjct: 1   MLSATAPA---TSLSAAAAHTTTTFLLHTPPLRLFLRRPPNPISASLN--PSPSPSPLVA 60

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 61  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 120

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT GAELLYEIRLL+AEKRA+AA
Sbjct: 121 ITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 180

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 181 RYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 240

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ARTAALEDALKV VTE ARSLIVR+LE EGEGK+  EE+ EAGVRVPVVRLKIGEVAEAT
Sbjct: 241 ARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEVEEEIEAGVRVPVVRLKIGEVAEAT 300

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 301 TVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVV 360

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           VAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT AT  G +G LKVQRG+ 
Sbjct: 361 VAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGAT--GSDGELKVQRGSE 420

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 421 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lcy03g005440 vs. ExPASy TrEMBL
Match: A0A6J1HLU0 (rubisco accumulation factor 1.1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465376 PE=4 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.1e-200
Identity = 368/454 (81.06%), Postives = 400/454 (88.11%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PATSL++ +A    T TTFL H+P  RLFLR  P  ISA LN  P PSPSPL A
Sbjct: 31  MLSATAPATSLSAAAAHTTTTTTTFLLHTPPLRLFLRHPPNLISASLN--PFPSPSPLVA 90

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 91  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 150

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT GAELLYEIRLL+AEKRA+AA
Sbjct: 151 ITGIGGVQQNTLVVGAQVRESLVQTINDTDPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 210

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YI++NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 211 RYIMENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 270

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ARTAAL DALKV VTE ARSLIVR+LE EGEGK+G EE+ EAGVRVPVVRLKIGEVAEAT
Sbjct: 271 ARTAALADALKVAVTEEARSLIVRDLEREGEGKDGAEEEIEAGVRVPVVRLKIGEVAEAT 330

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGV  APMEVRSEGEFGVVVAEEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 331 TVVVLPVCNGEEGGKGVREAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVV 390

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           V F DAR+LPWRVNRWYKEEAILVV DRSR EV A DGFY+T AT  G +G LKVQRG+ 
Sbjct: 391 VVFKDARSLPWRVNRWYKEEAILVVADRSRKEVEAGDGFYMTGAT--GNDGELKVQRGSE 450

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 451 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 480

BLAST of Lcy03g005440 vs. ExPASy TrEMBL
Match: A0A5D3DZE4 (Rubisco accumulation factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001420 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.5e-189
Identity = 361/461 (78.31%), Postives = 395/461 (85.68%), Query Frame = 0

Query: 1   MLSAT-TPATSLTSLSAAAAHTATTFLFHSP-----RLFLRRRPTPISAILNPSPSPSPS 60
           MLS+T T A SLTSLS + AHT   FL H P     RLFLRRR  PISAI+N    PSPS
Sbjct: 1   MLSSTITNANSLTSLS-SIAHTTAIFL-HQPIDSPLRLFLRRRSNPISAIIN----PSPS 60

Query: 61  PLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPP 120
           PLAAK QVYQPFRPPPSPLPPQYRSLDT GKL+VLSNRLGLWFEYAPLISSL+QEGFTPP
Sbjct: 61  PLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPP 120

Query: 121 TIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKR 180
           T+EEITGISGVQQN  +VGAQVR+SLVQ+ D SDPD+IA+FDTSGAELLYEIRLLS EKR
Sbjct: 121 TLEEITGISGVQQNTFVVGAQVRESLVQSND-SDPDVIASFDTSGAELLYEIRLLSTEKR 180

Query: 181 ASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYK 240
            +AAKYIV+NRLD KGAQDLARA+KDFPRR+GD+GWE FDYD  GDCLA+MYYRLSREY 
Sbjct: 181 VAAAKYIVENRLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYN 240

Query: 241 NQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEV 300
           N  E RTAALE+ALKVVVTE AR+LIV +LE +G+GK+G EE+  AGVRVPVVRLKIGEV
Sbjct: 241 NSSERRTAALEEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEV 300

Query: 301 AEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGR 360
           AEAT V+VMPVCKA EGEKGVG APMEVRSEG+FGVVVAE+GW RWVVLP WEP+ GL +
Sbjct: 301 AEATKVVVMPVCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVK 360

Query: 361 -GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGE--GVL 420
            GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   TV GG+  G L
Sbjct: 361 GGGVVVAFKDARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGEL 420

Query: 421 KVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           KV+RGNAL E GV+ESLGTV+LVVRPPK+MEDDQLSDEDWD
Sbjct: 421 KVERGNALTEMGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of Lcy03g005440 vs. ExPASy TrEMBL
Match: A0A1S3CHL8 (rubisco accumulation factor 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501013 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 7.7e-189
Identity = 360/461 (78.09%), Postives = 395/461 (85.68%), Query Frame = 0

Query: 1   MLSAT-TPATSLTSLSAAAAHTATTFLFHSP-----RLFLRRRPTPISAILNPSPSPSPS 60
           MLS+T T A SLTSLS + AHT   FL H P     RLFLRRR  PISAI+N    PSPS
Sbjct: 1   MLSSTITNANSLTSLS-SIAHTTAIFL-HQPIDSPLRLFLRRRSNPISAIIN----PSPS 60

Query: 61  PLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPP 120
           PLAAK QVYQPFRPPPSPLPPQYRSLDT GKL+VLSNRLGLWF+YAPLISSL+QEGFTPP
Sbjct: 61  PLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFQYAPLISSLLQEGFTPP 120

Query: 121 TIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKR 180
           T+EEITGISGVQQN  +VGAQVR+SLVQ+ D SDPD+IA+FDTSGAELLYEIRLLS EKR
Sbjct: 121 TLEEITGISGVQQNTFVVGAQVRESLVQSND-SDPDVIASFDTSGAELLYEIRLLSTEKR 180

Query: 181 ASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYK 240
            +AAKYIV+NRLD KGAQDLARA+KDFPRR+GD+GWE FDYD  GDCLA+MYYRLSREY 
Sbjct: 181 VAAAKYIVENRLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYN 240

Query: 241 NQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEV 300
           N  E RTAALE+ALKVVVTE AR+LIV +LE +G+GK+G EE+  AGVRVPVVRLKIGEV
Sbjct: 241 NSSERRTAALEEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEV 300

Query: 301 AEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGR 360
           AEAT V+VMPVCKA EGEKGVG APMEVRSEG+FGVVVAE+GW RWVVLP WEP+ GL +
Sbjct: 301 AEATKVVVMPVCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVK 360

Query: 361 -GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGE--GVL 420
            GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   TV GG+  G L
Sbjct: 361 GGGVVVAFKDARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGEL 420

Query: 421 KVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           KV+RGNAL E GV+ESLGTV+LVVRPPK+MEDDQLSDEDWD
Sbjct: 421 KVERGNALTEMGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of Lcy03g005440 vs. ExPASy TrEMBL
Match: A0A5A7VA63 (Rubisco accumulation factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00440 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.2e-188
Identity = 360/461 (78.09%), Postives = 394/461 (85.47%), Query Frame = 0

Query: 1   MLSAT-TPATSLTSLSAAAAHTATTFLFHSP-----RLFLRRRPTPISAILNPSPSPSPS 60
           MLS+T T A SLTSLS + AHT   FL H P     RLFLRRR  PISAI+N    PSPS
Sbjct: 1   MLSSTITNANSLTSLS-SIAHTTAIFL-HQPIDSPLRLFLRRRSNPISAIIN----PSPS 60

Query: 61  PLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPP 120
           PLAAK QVYQPFRPPPSPLPPQYRSLDT GKL+VLSNRLGLWFEYAPLISSL+QEGFTPP
Sbjct: 61  PLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPP 120

Query: 121 TIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKR 180
           T+EEITGISGVQQN  +VGAQVR+SLVQ+ D SDPD+IA+FDTSGAELLYEIRLLS EKR
Sbjct: 121 TLEEITGISGVQQNTFVVGAQVRESLVQSND-SDPDVIASFDTSGAELLYEIRLLSTEKR 180

Query: 181 ASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYK 240
            +AAKYIV+NRLD KGAQDLARA+KDFPRR+GD+GWE FDYD  GDCLA+MYYRLSREY 
Sbjct: 181 VAAAKYIVENRLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYN 240

Query: 241 NQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEV 300
           N  E RTAALE+ALKV VTE AR+LIV +LE +G+GK+G EE+  AGVRVPVVRLKIGEV
Sbjct: 241 NSSERRTAALEEALKVGVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEV 300

Query: 301 AEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGR 360
           AEAT V+VMPVCKA EGEKGVG APMEVRSEG+FGVVVAE+GW RWVVLP WEP+ GL +
Sbjct: 301 AEATKVVVMPVCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVK 360

Query: 361 -GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGE--GVL 420
            GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   TV GG+  G L
Sbjct: 361 GGGVVVAFKDARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGEL 420

Query: 421 KVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           KV+RGNAL E GV+ESLGTV+LVVRPPK+MEDDQLSDEDWD
Sbjct: 421 KVERGNALTEMGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of Lcy03g005440 vs. NCBI nr
Match: XP_022933155.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 729.9 bits (1883), Expect = 1.3e-206
Identity = 381/454 (83.92%), Postives = 407/454 (89.65%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PA   TSLSAAAAHT TTFL H+P  RLFLRR P PISA LN  PSPSPSPL A
Sbjct: 1   MLSATAPA---TSLSAAAAHTTTTFLLHTPPLRLFLRRPPNPISASLN--PSPSPSPLVA 60

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 61  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 120

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT GAELLYEIRLL+AEKRA+AA
Sbjct: 121 ITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 180

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 181 RYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 240

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ARTAALEDALKV VTE ARSLIVR+LE EGEGK+  EE+ EAGVRVPVVRLKIGEVAEAT
Sbjct: 241 ARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEVEEEIEAGVRVPVVRLKIGEVAEAT 300

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 301 TVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVV 360

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           VAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT AT  G +G LKVQRG+ 
Sbjct: 361 VAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGAT--GSDGELKVQRGSE 420

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 421 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lcy03g005440 vs. NCBI nr
Match: KAG6588314.1 (Rubisco accumulation factor 1.2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020875.1 Rubisco accumulation factor 1.2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 724.9 bits (1870), Expect = 4.2e-205
Identity = 379/454 (83.48%), Postives = 406/454 (89.43%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PA   TSLSAAAAHT TTFL H+P  RLFLRR P  ISA LN  PSPSPSPL A
Sbjct: 1   MLSATAPA---TSLSAAAAHTTTTFLLHTPPLRLFLRRPPNLISASLN--PSPSPSPLVA 60

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 61  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 120

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT G+ELLYEIRLL+AEKRA+AA
Sbjct: 121 ITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGSELLYEIRLLNAEKRAAAA 180

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 181 RYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 240

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ARTAALEDALKV VTE ARSLIVR+LE EGEGK+  EE+ EAGVRVPVVRLKIGEVAEAT
Sbjct: 241 ARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEAEEEIEAGVRVPVVRLKIGEVAEAT 300

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 301 TVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVV 360

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           VAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT AT  G +G LKVQRG+ 
Sbjct: 361 VAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGAT--GSDGELKVQRGSE 420

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 421 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lcy03g005440 vs. NCBI nr
Match: XP_023531170.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 715.7 bits (1846), Expect = 2.5e-202
Identity = 372/454 (81.94%), Postives = 402/454 (88.55%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PATSL++ +A    T TTFL H+P  RLFLRR P  ISA LN  P PSPSPL A
Sbjct: 1   MLSATAPATSLSAAAAHTTTTTTTFLLHTPPLRLFLRRPPNLISASLN--PYPSPSPLVA 60

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 61  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 120

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT GAELLYEIRLL+AEKRA+AA
Sbjct: 121 ITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 180

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 181 RYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 240

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ART+ALEDALKV VTE ARSLIVR+LE EGEGK+  EE+ EAGV+VPVVRLKIGEVAEAT
Sbjct: 241 ARTSALEDALKVAVTEEARSLIVRDLEREGEGKDEAEEEIEAGVKVPVVRLKIGEVAEAT 300

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGVG APMEVRSEGEFGVVV EEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 301 TVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVVEEGWRRWVVLPGWEPIAGLGKGGVV 360

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           VAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT AT  G +G LKVQRG+ 
Sbjct: 361 VAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGAT--GNDGELKVQRGSE 420

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 421 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 450

BLAST of Lcy03g005440 vs. NCBI nr
Match: XP_022965491.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 709.1 bits (1829), Expect = 2.4e-200
Identity = 368/454 (81.06%), Postives = 400/454 (88.11%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP--RLFLRRRPTPISAILNPSPSPSPSPLAA 60
           MLSAT PATSL++ +A    T TTFL H+P  RLFLR  P  ISA LN  P PSPSPL A
Sbjct: 31  MLSATAPATSLSAAAAHTTTTTTTFLLHTPPLRLFLRHPPNLISASLN--PFPSPSPLVA 90

Query: 61  KPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEE 120
           KPQVYQPFRPPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQEGFTPPTIEE
Sbjct: 91  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 150

Query: 121 ITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAA 180
           ITGI GVQQN L+VGAQVR+SLVQTI+++DPDLIAAFDT GAELLYEIRLL+AEKRA+AA
Sbjct: 151 ITGIGGVQQNTLVVGAQVRESLVQTINDTDPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 210

Query: 181 KYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPE 240
           +YI++NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YRLSREY N  E
Sbjct: 211 RYIMENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 270

Query: 241 ARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEAT 300
           ARTAAL DALKV VTE ARSLIVR+LE EGEGK+G EE+ EAGVRVPVVRLKIGEVAEAT
Sbjct: 271 ARTAALADALKVAVTEEARSLIVRDLEREGEGKDGAEEEIEAGVRVPVVRLKIGEVAEAT 330

Query: 301 TVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVV 360
           TV+V+PVC  EEG KGV  APMEVRSEGEFGVVVAEEGWRRWVVLP WEP+ GLG+GGVV
Sbjct: 331 TVVVLPVCNGEEGGKGVREAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVV 390

Query: 361 VAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNA 420
           V F DAR+LPWRVNRWYKEEAILVV DRSR EV A DGFY+T AT  G +G LKVQRG+ 
Sbjct: 391 VVFKDARSLPWRVNRWYKEEAILVVADRSRKEVEAGDGFYMTGAT--GNDGELKVQRGSE 450

Query: 421 LMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 451 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 480

BLAST of Lcy03g005440 vs. NCBI nr
Match: XP_038880540.1 (rubisco accumulation factor 1.1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 687.6 bits (1773), Expect = 7.4e-194
Identity = 369/458 (80.57%), Postives = 399/458 (87.12%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSP-----RLFLRRRPTPISAILNPSPSPSPSP 60
           MLS TT A SLTSLS AAAHT  TFL H P     RLFLRRRP  ISA LN      PSP
Sbjct: 1   MLSTTTTANSLTSLS-AAAHTTATFL-HPPRDSSLRLFLRRRPNSISATLN------PSP 60

Query: 61  LAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPT 120
           LAAK QVYQPFRPPPSPLP QYRSLD  GKL+VLSNRLGLWFEYAPLISSLIQEGFTPPT
Sbjct: 61  LAAKQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPT 120

Query: 121 IEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRA 180
           +EEITGI+GVQQNI +VGAQVR+SLVQ+ +N+DPD+IAAFDTSGAELLYEIRLLS EKRA
Sbjct: 121 LEEITGITGVQQNIFVVGAQVRESLVQS-NNADPDVIAAFDTSGAELLYEIRLLSTEKRA 180

Query: 181 SAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKN 240
           +AA+YIV+NRLD KGAQDLARA+KDFPRRRGDKGWE FDY+ PGDCLA+MYYRLSREY N
Sbjct: 181 AAARYIVENRLDIKGAQDLARALKDFPRRRGDKGWECFDYEEPGDCLAYMYYRLSREYNN 240

Query: 241 QPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVA 300
             E RTAALE+ALKVVVTE AR LIV +LEG+G+GKEG EE+ E G+RVPVVR+KIGEVA
Sbjct: 241 PSEPRTAALEEALKVVVTEKARDLIVGDLEGKGDGKEGVEEEIEVGIRVPVVRMKIGEVA 300

Query: 301 EATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGR- 360
           EATTV+VMPVCKA EGEKGVG APME+RSEGEFGVVVAE+ WR+WVVLP WEP+ GL + 
Sbjct: 301 EATTVLVMPVCKAGEGEKGVGEAPMELRSEGEFGVVVAEKEWRKWVVLPGWEPVVGLVKG 360

Query: 361 GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQ 420
           GGVVVAF DARALPWRVNRWYKEE ILVV DRSR EVVA DGFYLT ATV GGE  LKVQ
Sbjct: 361 GGVVVAFEDARALPWRVNRWYKEEPILVVVDRSRKEVVAGDGFYLTGATVGGGE--LKVQ 420

Query: 421 RGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 453
           RGNALME GV+ESLGTVLLVVRPPK+MEDDQLSDEDWD
Sbjct: 421 RGNALMEMGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 447

BLAST of Lcy03g005440 vs. TAIR 10
Match: AT5G28500.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1); Has 109 Blast hits to 109 proteins in 49 species: Archae - 0; Bacteria - 67; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-125
Identity = 256/454 (56.39%), Postives = 327/454 (72.03%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSPRLFLRRRPTPISAILNPSPSPSPSPLAAKP 60
           MLS T      T+LS++    + T  F + R   R+  T I++ L P+ +    P   K 
Sbjct: 1   MLSLTA-----TTLSSSIFTQSKTHGFFNTRPVYRKPFTTITSALIPASNRQAPP---KQ 60

Query: 61  QVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEIT 120
           Q+YQPFRPPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+T
Sbjct: 61  QLYQPFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELT 120

Query: 121 GISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKY 180
           GISGV+QN LIVGAQVR SLVQ+   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+Y
Sbjct: 121 GISGVEQNSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEY 180

Query: 181 IVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEAR 240
           IVD+  D KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E R
Sbjct: 181 IVDHGFDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIR 240

Query: 241 TAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEEDNEAGVRVPVVRLKIGEVAEATTV 300
           T  LE AL+  VTE A+  ++REL GE E +E  +E+    +RVPVVRL+ GEVA A++V
Sbjct: 241 TTLLEQALETAVTEKAKKAVLRELHGESE-EERVKEEEIKIIRVPVVRLRFGEVAGASSV 300

Query: 301 MVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVA 360
           +V+PVCKAEEGE+ +  APME  S GEFGVV AE+ W RWVVLP W+P+  + R GV V+
Sbjct: 301 VVLPVCKAEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAV-RKGVAVS 360

Query: 361 FNDAR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGGEGVLKVQRGNAL 420
           F+D R  LPW      K EAI+VV DR +  V A++G+Y         +G +K+ RG  L
Sbjct: 361 FSDDREVLPWN----GKGEAIMVVIDREKKTVEADNGYYYLVV----ADGGMKLDRGLVL 420

Query: 421 MEKGVRESLGTVLLVVRPPKDMEDD-QLSDEDWD 453
            EKGV ESLG V+LVVRPP+D +D+ Q++DEDWD
Sbjct: 421 KEKGVNESLGMVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of Lcy03g005440 vs. TAIR 10
Match: AT3G04550.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28500.1); Has 110 Blast hits to 110 proteins in 51 species: Archae - 0; Bacteria - 67; Metazoa - 1; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 441.8 bits (1135), Expect = 6.6e-124
Identity = 231/406 (56.90%), Postives = 302/406 (74.38%), Query Frame = 0

Query: 53  PSPLAAKPQVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFT 112
           P    A+ Q+YQPFRPP SP+P Q+RSLD+ GK+++L+ R+ LWFEYAPLISSL  +GFT
Sbjct: 55  PKNPPARQQLYQPFRPPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAPLISSLYTDGFT 114

Query: 113 PPTIEEITGISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAE 172
           PPTIEE+TGIS ++QN LIVGAQVR S++Q+I   +P+LI+AFDT GAELLYEIRLLS  
Sbjct: 115 PPTIEELTGISSIEQNRLIVGAQVRDSILQSI--HEPELISAFDTGGAELLYEIRLLSTT 174

Query: 173 KRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSRE 232
           +R +AA +I+D  +D KGAQDLARA+KD+P RRGD GW  FDY+ PGDCL+F+YYR SRE
Sbjct: 175 QRVAAATFIIDRNIDSKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCLSFLYYRQSRE 234

Query: 233 YKNQPEARTAALEDALKVVVTEGARSLIVRELEGEGEGKEGTE----EDNEAGVRVPVVR 292
            KN  + RT+ L  AL V  +E A++ +  EL G+ E ++  E    E+    +R+PVVR
Sbjct: 235 NKNPSDQRTSMLLQALGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEEVKAIRIPVVR 294

Query: 293 LKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEP 352
           LK GEVAEAT+V+V+PVCKAEEGEK +  APME+ + G+F VV AE+GW+RWVVLP+W P
Sbjct: 295 LKFGEVAEATSVVVLPVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWKRWVVLPSWNP 354

Query: 353 LRGLGRGGVVVAFNDAR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAATVEGG 412
           +  +G+GGV V+F D R  LPW      KEE +LVV DR RN V A+DG+YL  A     
Sbjct: 355 VAAIGKGGVAVSFRDDRKVLPWD----GKEEPLLVVADRVRNVVEADDGYYLVVA----- 414

Query: 413 EGVLKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDD-QLSDEDWD 453
           E  LK+++G+ L  + V+ESLG V+LVVRPP++ +DD Q S ++WD
Sbjct: 415 ENGLKLEKGSDLKAREVKESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of Lcy03g005440 vs. TAIR 10
Match: AT5G28500.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1). )

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-77
Identity = 161/276 (58.33%), Postives = 203/276 (73.55%), Query Frame = 0

Query: 1   MLSATTPATSLTSLSAAAAHTATTFLFHSPRLFLRRRPTPISAILNPSPSPSPSPLAAKP 60
           MLS T      T+LS++    + T  F + R   R+  T I++ L P+ +    P   K 
Sbjct: 1   MLSLTA-----TTLSSSIFTQSKTHGFFNTRPVYRKPFTTITSALIPASNRQAPP---KQ 60

Query: 61  QVYQPFRPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEIT 120
           Q+YQPFRPPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+T
Sbjct: 61  QLYQPFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELT 120

Query: 121 GISGVQQNILIVGAQVRQSLVQTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKY 180
           GISGV+QN LIVGAQVR SLVQ+   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+Y
Sbjct: 121 GISGVEQNSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEY 180

Query: 181 IVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEAR 240
           IVD+  D KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E R
Sbjct: 181 IVDHGFDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIR 240

Query: 241 TAALEDALKVVVTEGARSLIVRELEGEGEGKEGTEE 277
           T  LE AL+  VTE A+  ++REL GE E + G E+
Sbjct: 241 TTLLEQALETAVTEKAKKAVLRELHGESEEERGKED 266