Lcy03g004490 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g004490
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCalcium-binding EF hand family protein
LocationChr03: 21753115 .. 21753676 (-)
RNA-Seq ExpressionLcy03g004490
SyntenyLcy03g004490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATTAACGATACAAAGATATTCGAAGATTGCGTCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTACCTCAACGCCGATGAACTTGGCGTCGAGTTTTGCAATCAATCATTAGATCCGATGAAAGATATCTATGAGGTTGTTTGTCATAAGTTTCAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTACGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAACAAGATAGTTTTCTCACGAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGGAGAAAAATAGGAAAAACTATTTCCCTCTCAGAAGAAAAACTTCCATGATTTTCCTTGCCTATGTTCTTAGAAATAGGTACCATGATCCAATTGGATAGTCAAGATTTAGAACAGTTTTCCCAACCACATACTAATCATTTTCTTTTAAATTATCTAACTTTTATTATGAGTCGTTTGTTTTGTGCTAATCTTAG

mRNA sequence

ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATTAACGATACAAAGATATTCGAAGATTGCGTCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTACCTCAACGCCGATGAACTTGGCGTCGAGTTTTGCAATCAATCATTAGATCCGATGAAAGATATCTATGAGGTTGTTTGTCATAAGTTTCAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTACGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAACAAGATAGTTTTCTCACGAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGGAGAAAAATAGGAAAAACTATTTCCCTCTCAGAAGAAAAACTTCCATGATTTTCCTTGCCTATGTTCTTAGAAATAGGTACCATGATCCAATTGGATAGTCAAGATTTAGAACAGTTTTCCCAACCACATACTAATCATTTTCTTTTAAATTATCTAACTTTTATTATGAGTCGTTTGTTTTGTGCTAATCTTAG

Coding sequence (CDS)

ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATTAACGATACAAAGATATTCGAAGATTGCGTCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTACCTCAACGCCGATGAACTTGGCGTCGAGTTTTGCAATCAATCATTAGATCCGATGAAAGATATCTATGAGGTTGTTTGTCATAAGTTTCAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTACGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAACAAGATAGTTTTCTCACGAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGGAGAAAAATAG

Protein sequence

MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSLDPMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAVEHEKKKKGEK
Homology
BLAST of Lcy03g004490 vs. ExPASy TrEMBL
Match: A0A0A0LYD8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695380 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.5e-41
Identity = 91/127 (71.65%), Postives = 105/127 (82.68%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL V F +  L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDTDNDGFLNMNELRVGFRSHPLEFELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKFQV+K+GGID + F S+IRDILLAMAQGIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLSEVVCHKFQVEKSGGIDEEEFKSVIRDILLAMAQGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK ++
Sbjct: 121 ELEKARE 127

BLAST of Lcy03g004490 vs. ExPASy TrEMBL
Match: A0A5A7VAA7 (Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00860 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.7e-40
Identity = 91/127 (71.65%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL   F +Q L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA GIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lcy03g004490 vs. ExPASy TrEMBL
Match: A0A1S3CI80 (uncharacterized protein LOC103501058 OS=Cucumis melo OX=3656 GN=LOC103501058 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.7e-40
Identity = 91/127 (71.65%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL   F +Q L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA GIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lcy03g004490 vs. ExPASy TrEMBL
Match: A0A5D3B8A7 (Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1348G00080 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.8e-39
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL   F +Q L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID   F S++RDILLAMA GIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDENEFKSVVRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lcy03g004490 vs. ExPASy TrEMBL
Match: A0A6J1DWE2 (uncharacterized protein LOC111025096 OS=Momordica charantia OX=3673 GN=LOC111025096 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.3e-34
Identity = 77/122 (63.11%), Postives = 97/122 (79.51%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSLDPMKDI 60
           MSVAFLNDTT+ +FINDTKIF++CVKESF+KLD+D DG LNA+EL   F   S DP+ D+
Sbjct: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGF-RSSTDPVDDL 60

Query: 61  YEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAVEHEKK 120
            + VC KF V+K+GGI+   F S++ +ILLA+A GIGNLP+QVALQQD  L KAVEHE+ 
Sbjct: 61  SQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERA 120

Query: 121 KK 123
           K+
Sbjct: 121 KE 121

BLAST of Lcy03g004490 vs. NCBI nr
Match: XP_004142569.1 (uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical protein Csa_006967 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 5.3e-41
Identity = 91/127 (71.65%), Postives = 105/127 (82.68%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL V F +  L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDTDNDGFLNMNELRVGFRSHPLEFELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKFQV+K+GGID + F S+IRDILLAMAQGIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLSEVVCHKFQVEKSGGIDEEEFKSVIRDILLAMAQGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK ++
Sbjct: 121 ELEKARE 127

BLAST of Lcy03g004490 vs. NCBI nr
Match: XP_008462771.1 (PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 7.6e-40
Identity = 91/127 (71.65%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL   F +Q L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA GIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lcy03g004490 vs. NCBI nr
Match: TYJ95513.1 (calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 171.8 bits (434), Expect = 3.8e-39
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL-----D 60
           MSVAFLNDTT+TNFINDTKIF+DCVKESFKKLD D DG+LN +EL   F +Q L     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID   F S++RDILLAMA GIGN P+QVALQQDSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDENEFKSVVRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lcy03g004490 vs. NCBI nr
Match: XP_022158640.1 (uncharacterized protein LOC111025096 [Momordica charantia])

HSP 1 Score: 154.8 bits (390), Expect = 4.8e-34
Identity = 77/122 (63.11%), Postives = 97/122 (79.51%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSLDPMKDI 60
           MSVAFLNDTT+ +FINDTKIF++CVKESF+KLD+D DG LNA+EL   F   S DP+ D+
Sbjct: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGF-RSSTDPVDDL 60

Query: 61  YEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAVEHEKK 120
            + VC KF V+K+GGI+   F S++ +ILLA+A GIGNLP+QVALQQD  L KAVEHE+ 
Sbjct: 61  SQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERA 120

Query: 121 KK 123
           K+
Sbjct: 121 KE 121

BLAST of Lcy03g004490 vs. NCBI nr
Match: XP_034925333.1 (uncharacterized protein LOC118056988 [Populus alba])

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-22
Identity = 56/126 (44.44%), Postives = 82/126 (65.08%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFC--------NQ 60
           MS+A +N+TT+T F+ D K FE+CVKE F+ LDVDGDG L+  EL   FC        + 
Sbjct: 22  MSLAVVNETTVTTFVEDMKAFENCVKECFEMLDVDGDGVLSRSELREGFCKLMSLGHESS 81

Query: 61  SLDPMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLT 119
             + +  +++ +  +F  D+NG ID + F  +IR+++ AM +G+GN P+ VAL+ DS L 
Sbjct: 82  KKEKIDHLFDTIFDRFDEDQNGSIDPREFKGLIRELMAAMGRGLGNSPVLVALEIDSLLM 141

BLAST of Lcy03g004490 vs. TAIR 10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 75.5 bits (184), Expect = 3.4e-14
Identity = 43/136 (31.62%), Postives = 75/136 (55.15%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCNQSL------ 60
           M V  ++ +T+ +F++D + F+  V E F  LD++ DG L+  EL   F +  L      
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  -------DPMKDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVAL-- 120
                  D + ++Y+ +  KF  D++G +D++ F S ++ I+LA+A G+G+ PI + L  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

Query: 121 QQDSFLTKAVEHEKKK 122
             D+FL KA + E  K
Sbjct: 121 DDDNFLKKAADLEASK 136

BLAST of Lcy03g004490 vs. TAIR 10
Match: AT1G54530.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 67.4 bits (163), Expect = 9.3e-12
Identity = 42/124 (33.87%), Postives = 63/124 (50.81%), Query Frame = 0

Query: 6   LNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADEL--------GVEFCNQSLDPM 65
           +   TL  F++DTK FE    + F+ LD+D +G L+  EL         VE      D  
Sbjct: 6   ITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDET 65

Query: 66  KDIYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQDSFLTKAVEH 122
            ++Y  +  +F  D    +  K F  +I +IL AMA+GIGN P+ + +  D  + KAV H
Sbjct: 66  DNVYNAIFERFGED----LVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLIMKAVLH 125

BLAST of Lcy03g004490 vs. TAIR 10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-05
Identity = 33/113 (29.20%), Postives = 53/113 (46.90%), Query Frame = 0

Query: 6   LNDTTLTNFINDTKIFEDCVKESFKKLDVDGDGYLNADELGVEFCN---------QSLDP 65
           L+ + +   + + K+F+  V++ F++LD D DG L+  EL     +         Q   P
Sbjct: 15  LDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGTSP 74

Query: 66  MKD-IYEVVCHKFQVDKNGGIDVKGFTSIIRDILLAMAQGIGNLPIQVALQQD 109
             D IY  V ++F       +    F  ++ DILL MA G+   PI V L+ D
Sbjct: 75  DSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPI-VILRMD 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LYD82.5e-4171.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695380 PE=4 SV=1[more]
A0A5A7VAA73.7e-4071.65Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CI803.7e-4071.65uncharacterized protein LOC103501058 OS=Cucumis melo OX=3656 GN=LOC103501058 PE=... [more]
A0A5D3B8A71.8e-3970.08Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1DWE22.3e-3463.11uncharacterized protein LOC111025096 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
Match NameE-valueIdentityDescription
XP_004142569.15.3e-4171.65uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical ... [more]
XP_008462771.17.6e-4071.65PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 cal... [more]
TYJ95513.13.8e-3970.08calcium-binding EF hand family protein [Cucumis melo var. makuwa][more]
XP_022158640.14.8e-3463.11uncharacterized protein LOC111025096 [Momordica charantia][more]
XP_034925333.12.5e-2244.44uncharacterized protein LOC118056988 [Populus alba][more]
Match NameE-valueIdentityDescription
AT2G44310.13.4e-1431.62Calcium-binding EF-hand family protein [more]
AT1G54530.19.3e-1233.87Calcium-binding EF hand family protein [more]
AT4G38810.21.7e-0529.20Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 4..99
e-value: 8.7E-9
score: 37.4
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 1..121
NoneNo IPR availablePANTHERPTHR34574:SF12CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..121
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 10.245409
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 14..94

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g004490.1Lcy03g004490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding