Lcy03g004390 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g004390
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationChr03: 21216556 .. 21219901 (-)
RNA-Seq ExpressionLcy03g004390
SyntenyLcy03g004390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAATGAGAGAAGAAACGAGAAATAAATTTAATTTAATTTACTTCTCACTCTGAAAAGTGAAAACACCCTCTCACTTTCATATTCATTCAACGCCTTTTTCTCTTCTCTCTTCTTCTGCAAACCCAAAACATCAAACATGTGCTTTCCAAACACTTCTCTCCCCAGAATCTGAGGAGGAACCCAGGATTTGCACCGCCAATCCGATCTAATCCAATCAAATGGACGATACCCATTCATTCAATGGTTTCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGAGTACTCCTCTTTCTCTCTCTCTTTCTCTCCTCTCTTCATCCGCTTTCATTCTCATTCTCATTCTATTCTCTTCTTTCTTGCAGTACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTGTATGAACCAACCAATTCACCCTCTCCCTCCTCTCCTTTGCATTTCTCTAATTCCTATCCATTTTCCGATTTTATTACATTTTCCCTTCGTTTTCAGAATTCCATCTCTTCTGCGTCTTTGATTGTTGTTCGTTTGCTTCTCTTTCTCTTGGAGCGCCTAATAATTGCGTTTCGTTTTGTTAATCTGATTTAGTTTTTGGGAATGAGAAATTCGATTGAGGGTTTCTTTTTTTTTTTTTTTTGGTTTTTTTTTTTTTTTTTGTTGCAGTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAATATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTAAGAATGATTTTCAGCATTGATCTTATCTTACACCCTTCACTTTCTATTCTACTGATTCTAGCCTTCTCCCTTTTTGATTTCTGCTAAATTTCTGGTTGGTTGGTTCTGTTCTGACAGGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGTATGTTTATCTTCTTCAAATCCTGTGAAATTTTGGCAATAGATTCTACTTCTGGAAAGGATGATCTTGCTTTCTGATTTTAAATGCCCTTTCAGTGAATCTTTTTCCTTGAATTGTTGGTGAGTTTTGTGAGAGAAGTAAGTCCTCTCGTTAGATGTTTCTTACTGTTGTTATTACATTACAGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGTAAGATCTCCCTCTCTCAATGGGCATGTATTCTTCTTTAATCTTTTATGTGCGGGAAGAAAAAATCGATTCCTTAACATGCTTTCTACTTGTAGGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTGTCGTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGTAAGACACATTTATTTTCCTCCTTTCGTATTTCGTCATGGAGTGCTTGCCTTCTATAGTTACACAAACATTAACTCGAACGATCGGGTAAATGTGCAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAGTGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTGTGGTAGATAGCACACCAAAAGTGTCCAGCCAGCATTATCAGCGAAACTCAGTCCATGAAAACCAAGCTCTTGATTCTCGTAGTTTTAGGCGAAGCCATTCTGATCAAAATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAAGAAAGAAAAGTTACTCGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCCTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGAGAAAACTTAGAGAGATCAGTTGGAACTTCTCTTGCAGCCCCTCTAGGTTGCAACAAACAATAGAGGGCGCAGCTGAAAACGCGGTTTTTAACGAACAAGATTGTGAAGATTGAAATCATAGTTGGTGTGAGTCATGTCGTTATAGCTGCCCCGTTCGGACGCCCAAAGGGCCCGAAATTGTGAGGCGTTTGTTAGATTCTTCAACTGCAGAGTATATATTCAAGAGTTAAGATTACAGGCACATTGCCCCAAACACTCTACAGAACAACTCTCTACCAGTTGGTGCTGTTGTGTTGTACAGAAATATGTAAGTTACTCTCAGCAGTTCTGAAACTTTTTTCACTTACAGTGACACTTTTTTTTTTTCCTTCTTTTTCTTGATCATTGTTTTAGTTTATAAGTTATTAGAAATTGTAATGGCTATTCTCTTA

mRNA sequence

GAGAAATGAGAGAAGAAACGAGAAATAAATTTAATTTAATTTACTTCTCACTCTGAAAAGTGAAAACACCCTCTCACTTTCATATTCATTCAACGCCTTTTTCTCTTCTCTCTTCTTCTGCAAACCCAAAACATCAAACATGTGCTTTCCAAACACTTCTCTCCCCAGAATCTGAGGAGGAACCCAGGATTTGCACCGCCAATCCGATCTAATCCAATCAAATGGACGATACCCATTCATTCAATGGTTTCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGATACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAATATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTGTCGTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAGTGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTGTGGTAGATAGCACACCAAAAGTGTCCAGCCAGCATTATCAGCGAAACTCAGTCCATGAAAACCAAGCTCTTGATTCTCGTAGTTTTAGGCGAAGCCATTCTGATCAAAATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAAGAAAGAAAAGTTACTCGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCCTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGAGAAAACTTAGAGAGATCAGTTGGAACTTCTCTTGCAGCCCCTCTAGGTTGCAACAAACAATAGAGGGCGCAGCTGAAAACGCGGTTTTTAACGAACAAGATTGTGAAGATTGAAATCATAGTTGGTGTGAGTCATGTCGTTATAGCTGCCCCGTTCGGACGCCCAAAGGGCCCGAAATTGTGAGGCGTTTGTTAGATTCTTCAACTGCAGAGTATATATTCAAGAGTTAAGATTACAGGCACATTGCCCCAAACACTCTACAGAACAACTCTCTACCAGTTGGTGCTGTTGTGTTGTACAGAAATATGTAAGTTACTCTCAGCAGTTCTGAAACTTTTTTCACTTACAGTGACACTTTTTTTTTTTCCTTCTTTTTCTTGATCATTGTTTTAGTTTATAAGTTATTAGAAATTGTAATGGCTATTCTCTTA

Coding sequence (CDS)

ATGGACGATACCCATTCATTCAATGGTTTCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGATACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAATATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTGTCGTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAGTGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTGTGGTAGATAGCACACCAAAAGTGTCCAGCCAGCATTATCAGCGAAACTCAGTCCATGAAAACCAAGCTCTTGATTCTCGTAGTTTTAGGCGAAGCCATTCTGATCAAAATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAAGAAAGAAAAGTTACTCGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCCTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGA

Protein sequence

MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCEDKFASQIHWEIRWLWN
Homology
BLAST of Lcy03g004390 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 649.4 bits (1674), Expect = 4.0e-185
Identity = 336/548 (61.31%), Postives = 393/548 (71.72%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D   Y E+DPTGRYGRY+ +LGKGASKTVYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQYR KH RVN+ AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KP+ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQP 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQ+DD        D+      + +   QP
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDY-----SLMNYLRQP 312

Query: 318 FLEQEKS---------LSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQLENLDNSI 377
           +LE   S           SI+      D W C       DGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIA+ +GH+RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILN 497
            IDGE+++LVP+W+PGPGI+E+   +Y   C S              N  S   LY  ++
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTYCHNCGS--------------NVSSCGSLYAYMS 492

Query: 498 CSTDECATADRH--FEQVSLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSF--- 551
            +   C  A+ H  FE+++ +A+     + DS         Q   V + +A+ S  F   
Sbjct: 493 SAARGCQCAELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSNGFVQM 541

BLAST of Lcy03g004390 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 4.0e-185
Identity = 336/548 (61.31%), Postives = 393/548 (71.72%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D   Y E+DPTGRYGRY+ +LGKGASKTVYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQYR KH RVN+ AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KP+ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQP 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQ+DD        D+      + +   QP
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDY-----SLMNYLRQP 312

Query: 318 FLEQEKS---------LSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQLENLDNSI 377
           +LE   S           SI+      D W C       DGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIA+ +GH+RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILN 497
            IDGE+++LVP+W+PGPGI+E+   +Y   C S              N  S   LY  ++
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTYCHNCGS--------------NVSSCGSLYAYMS 492

Query: 498 CSTDECATADRH--FEQVSLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSF--- 551
            +   C  A+ H  FE+++ +A+     + DS         Q   V + +A+ S  F   
Sbjct: 493 SAARGCQCAELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSNGFVQM 541

BLAST of Lcy03g004390 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 7.7e-181
Identity = 351/642 (54.67%), Postives = 425/642 (66.20%), Query Frame = 0

Query: 17  PDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S +VE+DPTGRYGRY+++LGKGASKTVYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTANRNINFVTE+FTSGTLRQYRL+H+RVN+RA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKPDALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQ 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  D     D +     Q
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDME---DSVGPLYRQ 308

Query: 317 P-----FLEQEKSLSSIN------------------YS------LEASDEWRCRTVPT-V 376
           P     +     + SS+N                  YS       E  + W      T  
Sbjct: 309 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 368

Query: 377 PDGIELFEDEDNDQLE------NLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDT 436
             GIELFE  +ND  E      N+D +IKGK R+DG + LRLRIA+KEG +RNIYFPFD 
Sbjct: 369 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 428

Query: 437 KNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQ 496
           + DTAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +      
Sbjct: 429 ETDTALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAA 488

Query: 497 SY---DASNHPSENHLIGNKRSGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVD 556
           S      SN  S   ++   R+      ++ C  + C      FE++++           
Sbjct: 489 SICNNCVSNRTSMGSVMDFLRTNPGA-NVIQCCRNGCGETHGRFEEITIR---------- 548

Query: 557 STPKVSSQHYQRNSVHENQALDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVI- 613
            T     + ++     E++ L S     +HS++            +++E L +    +  
Sbjct: 549 ETEVRLRELWKLQQQQESRELSSIDSGHNHSEEE-----------EEEEVLYEDPENMFS 608

BLAST of Lcy03g004390 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 4.0e-161
Identity = 290/437 (66.36%), Postives = 339/437 (77.57%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S YVE+DPTGRYGRY+++LGKG+SKTVYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT NRNINFVTEMFTSGTLRQYRLKH+RVN+RAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKPD L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFR--EELDGIASTTA 317
           VKDP +R F+EKCLA VS RLSA ELL D FL ID+       SD R  E   G+     
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDE-------SDMRRVESEKGLIDEAG 310

Query: 318 QPFLEQE--KSLSSINYSLEASDEWRCRTVPTVP----DGIELFEDEDNDQLE----NLD 377
            P          S+  YSL   ++W      TV     D +E   D+D ++ +    ++D
Sbjct: 311 TPLRHSYHIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVD 370

Query: 378 NSIKGKIREDG-SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVI 437
            SIKGK R++G  + LRL+   KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV 
Sbjct: 371 ISIKGKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVT 430

Query: 438 KIAEKIDGEIASLVPEW 442
           KIA  IDGEIASLVP W
Sbjct: 431 KIANMIDGEIASLVPNW 440

BLAST of Lcy03g004390 vs. ExPASy Swiss-Prot
Match: Q8S8Y9 (Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 5.2e-161
Identity = 307/548 (56.02%), Postives = 376/548 (68.61%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRYD+ILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKP+A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLSDFREELDGIASTTAQ 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  D+    D        
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 318 PFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDG 377
           P ++         Y  +      C         I+LF ++D D   ++D SIKGK   D 
Sbjct: 310 PLIDDPL------YHDQFESSQICE--------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 378 SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIAS 437
            I LRLRI++ EG IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 429

Query: 438 LVPEWK----PGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILNCSTD 497
           LVP+WK        ++     +  G C    ASN   +  +   ++S    ++  +    
Sbjct: 430 LVPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDK 489

Query: 498 ECATADRHFEQV-SLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSFRRSHSDQN 557
            C++    F  +  L           S+ KV S  +  N +      + R  +  H  Q 
Sbjct: 490 SCSSVHGRFADMWGLRESYSDDGEKQSSRKVRSGRWSENEMRR----ELRWLKARHKIQL 535

Query: 558 YKKTGQSI 560
            K  GQ+I
Sbjct: 550 MKMRGQTI 535

BLAST of Lcy03g004390 vs. ExPASy TrEMBL
Match: A0A6J1KJY6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495945 PE=4 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.4e-302
Identity = 529/615 (86.02%), Postives = 557/615 (90.57%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    EL+GIA TTA PFLE++ S SSINY LEASDEWRCR+V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD PP+PVV+S PKVSSQHYQ NSV  EN AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. ExPASy TrEMBL
Match: A0A6J1F099 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441166 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 9.2e-302
Identity = 527/615 (85.69%), Postives = 556/615 (90.41%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWRC +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD+PP+PVV+S PKVSSQHYQ NSV  E+ AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. ExPASy TrEMBL
Match: A0A1S3CI45 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103501021 PE=4 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.9e-299
Identity = 529/617 (85.74%), Postives = 554/617 (89.79%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           MD T S   FS LL  PDFSHYVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRR N+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAIL+KSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           SD R ELD  AST  +PFLE+EK  SSI+YSLEASDEWR R+V    DGIELFED DNDQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLD +IKGKIREDGSIVLRLRI +KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY+ASN PS+N LI NK +G
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHF--EQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQA 540
            KLYQILN STD  A A +HF  EQ SL+AD+P QPVV S P VSSQHYQ +SV  ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQA 540

Query: 541 LDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           L SRSFR+ H D NYKKT QS+AVG  KEKL D KATVI  TS RSLLGSRSLS VSSYC
Sbjct: 541 LSSRSFRQRHYDDNYKKTDQSLAVG-YKEKLPDKKATVI-DTSQRSLLGSRSLSRVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 615

BLAST of Lcy03g004390 vs. ExPASy TrEMBL
Match: A0A6J1DKV6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020998 PE=4 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 4.0e-297
Identity = 520/615 (84.55%), Postives = 549/615 (89.27%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S +GFS  LHHPD S YVEIDP+GRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   MEKDESIDGFSN-LHHPDSSRYVEIDPSGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCN LQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSG+L
Sbjct: 61  NQVKLCNLLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGSL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVN RAVKRWCRQILR LLYLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNNRAVKRWCRQILRALLYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHAD CVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADRCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV SG+KP ALYKVKDPSMRQFVEKCLAPV  RLSARELLSDPFLQIDDCESKSK+
Sbjct: 241 QIYKKVTSGEKPAALYKVKDPSMRQFVEKCLAPVPYRLSARELLSDPFLQIDDCESKSKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           SD + ELD IAST  QPFLE+E S SSINYSL+ SDEWRCR V T PDG ELFED+D+DQ
Sbjct: 301 SDCQGELDRIASTIVQPFLEREISFSSINYSLDTSDEWRCRIVETEPDGTELFEDDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LEN+DNSI GKIREDGSIVLRLRIA+KEG IRNIYFPFDTK DTALTVATEMIAELDITD
Sbjct: 361 LENVDNSITGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKMDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDV KIAEKIDGEIASLVPEWKPGPGIDETPRISYDG  QSYD SNHPS NHLI   ++G
Sbjct: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGDLQSYDTSNHPSYNHLIEKMQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
           AK  QILNCS DE A+A RHFEQVS + DQP QPVV+  PKVS+QHYQ NSV HE QAL 
Sbjct: 481 AKNSQILNCSADERASAPRHFEQVSHKEDQPTQPVVERIPKVSNQHYQLNSVLHEGQALS 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR+SHSDQN KKTGQ I  G+  EK+  SKAT IA++S+RSLLGSRSLS V SY ED
Sbjct: 541 SHSFRQSHSDQNNKKTGQLITGGNDIEKIQVSKATAIAKSSIRSLLGSRSLSRVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIHWEIRWLWN
Sbjct: 601 KFASQIHWEIRWLWN 614

BLAST of Lcy03g004390 vs. ExPASy TrEMBL
Match: A0A0A0M0J2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G695390 PE=4 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.9e-295
Identity = 522/617 (84.60%), Postives = 547/617 (88.65%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           MD T S   FS LL  PDFSHYVEIDPTGRYGRYD+ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYDEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRR N+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDGCESKLKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           SD R ELD  AST  +PFLE+EK  SSI+YSLE SDEWR R+V   PDGIELFED DNDQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEGSDEWRYRSVQKEPDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           L +LDN+IKGKIREDGSIVLRLRI +KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LVSLDNNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY+A N PS+N LI NK +G
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNACNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHF--EQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQA 540
            KLYQILN STD  A A  HF  EQ SL+AD+P Q      P VSSQHYQ +SV +ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHEHFEQEQFSLKADRPTQ------PNVSSQHYQPDSVLNENQA 540

Query: 541 LDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           L S SFR+ HSD NYKK  QS+ VG  KEKL  +KATVI  TS RSLLGSRSLSTVSSYC
Sbjct: 541 LSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPVNKATVI-DTSQRSLLGSRSLSTVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 610

BLAST of Lcy03g004390 vs. NCBI nr
Match: XP_038880702.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.7e-302
Identity = 528/617 (85.58%), Postives = 556/617 (90.11%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           MD T S +GFS L+  PDFS YVEIDPTGRYGRY++ILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVN+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQ+DD ESKSK+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S+ R ELD  AST A PFLEQEK  SSI+YS E SDEWRC TV    DGIELFED+DND 
Sbjct: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVP+WKPGPGIDETPRISYDGG +SY+ SN PS+N LI NK +G
Sbjct: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHFE--QVSLEADQPPQPVVDSTPKVSSQHYQRNS-VHENQA 540
           AKLYQILNCS D  A    HFE  Q SL+ADQP Q VV+S PKVSSQH Q NS +HENQA
Sbjct: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540

Query: 541 LDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           L S SFR+ HS +NYKKT QS+ VG  KEK+ D+KATVI RTSLRSLLGSRSLSTV SYC
Sbjct: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 617

BLAST of Lcy03g004390 vs. NCBI nr
Match: KAG6588353.1 (putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022205.1 putative serine/threonine-protein kinase WNK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1048.5 bits (2710), Expect = 2.2e-302
Identity = 529/615 (86.02%), Postives = 558/615 (90.73%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQI+DCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQINDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWRCR+V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYHLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD+PP+PVV+S PKVSSQHYQ NSV  E+ AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. NCBI nr
Match: XP_023001906.1 (probable serine/threonine-protein kinase WNK9 [Cucurbita maxima])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.9e-302
Identity = 529/615 (86.02%), Postives = 557/615 (90.57%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    EL+GIA TTA PFLE++ S SSINY LEASDEWRCR+V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD PP+PVV+S PKVSSQHYQ NSV  EN AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. NCBI nr
Match: XP_022933886.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.9e-301
Identity = 527/615 (85.69%), Postives = 556/615 (90.41%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWRC +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD+PP+PVV+S PKVSSQHYQ NSV  E+ AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. NCBI nr
Match: XP_023529957.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.5e-301
Identity = 528/615 (85.85%), Postives = 556/615 (90.41%), Query Frame = 0

Query: 1   MDDTHSFNGFSTLLHHPDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAW 60
           M+   S  GFS LL  PDFS YVEIDPTGRYGRY++ILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQ 360
           S    ELD IA TTA PFLE++ S SSINY LEASDEWRC +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDCIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN ++G
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSV-HENQALD 540
            KLYQILN ST E A+    F+Q SL+AD+PP+PVV+S PKVSSQHYQ NSV  E+ AL 
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCED 600
           S SFR SHS+QN+KK GQSI VGD KEK+LD  A  IARTSLRSLLGSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Lcy03g004390 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 635.2 bits (1637), Expect = 5.5e-182
Identity = 351/642 (54.67%), Postives = 425/642 (66.20%), Query Frame = 0

Query: 17  PDFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S +VE+DPTGRYGRY+++LGKGASKTVYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTANRNINFVTE+FTSGTLRQYRL+H+RVN+RA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKPDALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQ 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  D     D +     Q
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDME---DSVGPLYRQ 308

Query: 317 P-----FLEQEKSLSSIN------------------YS------LEASDEWRCRTVPT-V 376
           P     +     + SS+N                  YS       E  + W      T  
Sbjct: 309 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 368

Query: 377 PDGIELFEDEDNDQLE------NLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDT 436
             GIELFE  +ND  E      N+D +IKGK R+DG + LRLRIA+KEG +RNIYFPFD 
Sbjct: 369 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 428

Query: 437 KNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQ 496
           + DTAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +      
Sbjct: 429 ETDTALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAA 488

Query: 497 SY---DASNHPSENHLIGNKRSGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPVVD 556
           S      SN  S   ++   R+      ++ C  + C      FE++++           
Sbjct: 489 SICNNCVSNRTSMGSVMDFLRTNPGA-NVIQCCRNGCGETHGRFEEITIR---------- 548

Query: 557 STPKVSSQHYQRNSVHENQALDSRSFRRSHSDQNYKKTGQSIAVGDKKEKLLDSKATVI- 613
            T     + ++     E++ L S     +HS++            +++E L +    +  
Sbjct: 549 ETEVRLRELWKLQQQQESRELSSIDSGHNHSEEE-----------EEEEVLYEDPENMFS 608

BLAST of Lcy03g004390 vs. TAIR 10
Match: AT3G04910.2 (with no lysine (K) kinase 1 )

HSP 1 Score: 581.3 bits (1497), Expect = 9.4e-166
Identity = 328/613 (53.51%), Postives = 397/613 (64.76%), Query Frame = 0

Query: 46  VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN 105
           +YRAFDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN
Sbjct: 15  LYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTAN 74

Query: 106 RNINFVTEMFTSGTLRQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDN 165
           RNINFVTE+FTSGTLRQYRL+H+RVN+RA+K WCRQILRGL YLHS +PPVIHRDLKCDN
Sbjct: 75  RNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDN 134

Query: 166 IFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLE 225
           IFVNGNQGE+KIGDLGLAAILRKSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LE
Sbjct: 135 IFVNGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILE 194

Query: 226 MVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 285
           MVTF+YPYSEC HP QIYKKV+SGKKPDALYKVKDP ++ F+EKCLA VS R+SARELL 
Sbjct: 195 MVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLD 254

Query: 286 DPFLQIDDCESKSKLSDFREELDGIASTTAQP-----FLEQEKSLSSIN----------- 345
           DPFL+IDD E   +  D     D +     QP     +     + SS+N           
Sbjct: 255 DPFLRIDDGEFDLRSVDME---DSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSN 314

Query: 346 -------YS------LEASDEWRCRTVPT-VPDGIELFEDEDNDQLE------NLDNSIK 405
                  YS       E  + W      T    GIELFE  +ND  E      N+D +IK
Sbjct: 315 SSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIK 374

Query: 406 GKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEK 465
           GK R+DG + LRLRIA+KEG +RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  
Sbjct: 375 GKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANM 434

Query: 466 IDGEIASLVPEWKPGPGIDETPRISYDGGCQSY---DASNHPSENHLIGNKRSGAKLYQI 525
           IDGEI+SLVP W+PGP  +E    +      S      SN  S   ++   R+      +
Sbjct: 435 IDGEISSLVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPGA-NV 494

Query: 526 LNCSTDECATADRHFEQVSLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSFRRS 585
           + C  + C      FE++++            T     + ++     E++ L S     +
Sbjct: 495 IQCCRNGCGETHGRFEEITIR----------ETEVRLRELWKLQQQQESRELSSIDSGHN 554

Query: 586 HSDQNYKKTGQSIAVGDKKEKLLDSKATVI---ARTSLRSLLGSRSLSTV-SSYCE---D 613
           HS++            +++E L +    +    A   +  + GS S S + S YC+   +
Sbjct: 555 HSEEE-----------EEEEVLYEDPENMFSCEAGNEINHISGSGSFSFMPSKYCDEPSE 602

BLAST of Lcy03g004390 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.8e-162
Identity = 290/437 (66.36%), Postives = 339/437 (77.57%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S YVE+DPTGRYGRY+++LGKG+SKTVYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT NRNINFVTEMFTSGTLRQYRLKH+RVN+RAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKPD L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFR--EELDGIASTTA 317
           VKDP +R F+EKCLA VS RLSA ELL D FL ID+       SD R  E   G+     
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDE-------SDMRRVESEKGLIDEAG 310

Query: 318 QPFLEQE--KSLSSINYSLEASDEWRCRTVPTVP----DGIELFEDEDNDQLE----NLD 377
            P          S+  YSL   ++W      TV     D +E   D+D ++ +    ++D
Sbjct: 311 TPLRHSYHIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVD 370

Query: 378 NSIKGKIREDG-SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVI 437
            SIKGK R++G  + LRL+   KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV 
Sbjct: 371 ISIKGKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVT 430

Query: 438 KIAEKIDGEIASLVPEW 442
           KIA  IDGEIASLVP W
Sbjct: 431 KIANMIDGEIASLVPNW 440

BLAST of Lcy03g004390 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 569.3 bits (1466), Expect = 3.7e-162
Identity = 307/548 (56.02%), Postives = 376/548 (68.61%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRYD+ILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKP+A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLSDFREELDGIASTTAQ 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  D+    D        
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 318 PFLEQEKSLSSINYSLEASDEWRCRTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDG 377
           P ++         Y  +      C         I+LF ++D D   ++D SIKGK   D 
Sbjct: 310 PLIDDPL------YHDQFESSQICE--------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 378 SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIAS 437
            I LRLRI++ EG IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 429

Query: 438 LVPEWK----PGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILNCSTD 497
           LVP+WK        ++     +  G C    ASN   +  +   ++S    ++  +    
Sbjct: 430 LVPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDK 489

Query: 498 ECATADRHFEQV-SLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSFRRSHSDQN 557
            C++    F  +  L           S+ KV S  +  N +      + R  +  H  Q 
Sbjct: 490 SCSSVHGRFADMWGLRESYSDDGEKQSSRKVRSGRWSENEMRR----ELRWLKARHKIQL 535

Query: 558 YKKTGQSI 560
            K  GQ+I
Sbjct: 550 MKMRGQTI 535

BLAST of Lcy03g004390 vs. TAIR 10
Match: AT3G22420.2 (with no lysine (K) kinase 2 )

HSP 1 Score: 538.1 bits (1385), Expect = 9.2e-153
Identity = 307/607 (50.58%), Postives = 376/607 (61.94%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRYDQILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRYD+ILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCV-- 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CV  
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 ------------------------------GTPEFMAPEVYEEAYNELVDIYSFGMCVLE 257
                                         GTPEFMAPEVY+E YNELVD+Y+FGMCVLE
Sbjct: 190 SKPSHHWNFIALIMFFTTLDLPLLCLCVVKGTPEFMAPEVYDEEYNELVDVYAFGMCVLE 249

Query: 258 MVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 317
           MVTF+YPYSEC HP QIYKKV SGKKP+A Y VKDP +R+FVEKCLA V+CRL+A ELL 
Sbjct: 250 MVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQ 309

Query: 318 DPFLQIDDCES-KSKLSDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRCRTVP 377
           DPFLQ D+ +    +  D+    D        P ++         Y  +      C    
Sbjct: 310 DPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHPLIDDPL------YHDQFESSQICE--- 369

Query: 378 TVPDGIELFEDEDNDQLENLDNSIKGKIREDGSIVLRLRIAEKE---------------- 437
                I+LF ++D D   ++D SIKGK   D  I LRLRI++ E                
Sbjct: 370 -----IDLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGIVSIFFDSFKIIGLK 429

Query: 438 -----------GHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASL 497
                      G IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+L
Sbjct: 430 NVTRFRVLGNVGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAAL 489

Query: 498 VPEWK----PGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRSGAKLYQILNCSTDE 557
           VP+WK        ++     +  G C    ASN   +  +   ++S    ++  +     
Sbjct: 490 VPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDKS 549

Query: 558 CATADRHFEQV-SLEADQPPQPVVDSTPKVSSQHYQRNSVHENQALDSRSFRRSHSDQNY 560
           C++    F  +  L           S+ KV S  +  N +      + R  +  H  Q  
Sbjct: 550 CSSVHGRFADMWGLRESYSDDGEKQSSRKVRSGRWSENEMRR----ELRWLKARHKIQLM 594

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2YMV64.0e-18561.31Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3... [more]
Q0D5984.0e-18561.31Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX... [more]
Q9CAV67.7e-18154.67Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
Q2V3384.0e-16166.36Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y95.2e-16156.02Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KJY61.4e-30286.02Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F0999.2e-30285.69Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3CI451.9e-29985.74Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1DKV64.0e-29784.55Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0M0J22.9e-29584.60Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
Match NameE-valueIdentityDescription
XP_038880702.11.7e-30285.58probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida][more]
KAG6588353.12.2e-30286.02putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma s... [more]
XP_023001906.12.9e-30286.02probable serine/threonine-protein kinase WNK9 [Cucurbita maxima][more]
XP_022933886.11.9e-30185.69probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata][more]
XP_023529957.12.5e-30185.85probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
AT3G04910.15.5e-18254.67with no lysine (K) kinase 1 [more]
AT3G04910.29.4e-16653.51with no lysine (K) kinase 1 [more]
AT5G28080.22.8e-16266.36Protein kinase superfamily protein [more]
AT3G22420.13.7e-16256.02with no lysine (K) kinase 2 [more]
AT3G22420.29.2e-15350.58with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 32..289
e-value: 1.6E-59
score: 213.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 35..289
e-value: 3.7E-49
score: 167.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 32..289
score: 38.590778
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 379..433
e-value: 3.2E-5
score: 23.9
NoneNo IPR availableGENE3D3.10.20.90coord: 376..475
e-value: 1.5E-5
score: 27.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 18..113
e-value: 1.3E-32
score: 113.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 115..295
e-value: 1.7E-49
score: 169.8
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 80..302
e-value: 2.4E-19
score: 67.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 508..556
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 516..537
NoneNo IPR availablePANTHERPTHR13902:SF144SERINE/THREONINE-PROTEIN KINASE WNK1coord: 14..614
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 14..614
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 30..289
e-value: 4.45072E-171
score: 484.807
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 156..168
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 25..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g004390.1Lcy03g004390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity