Lcy03g001310 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g001310
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionExonuclease domain-containing protein
LocationChr03: 4200193 .. 4202568 (-)
RNA-Seq ExpressionLcy03g001310
SyntenyLcy03g001310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGATTTCCATGTCTCCGAAAAGCACGTCTCCATTTTCTTCTCCACGATTTACAGCCTCTCTCTCTCTCTCTCTCAACTTACTTTCTTCTGTTAGGTTCTCAAATGCAGCATAATCCTTCCTCTGATTTCCTTTCAGGCAAGTTTCTCTCTCTCTATCTCTCTTCGTTTTGGAAGTTCCCTGTTTCTGCATTGAACAAATGGTACAAAATGCGAATAAGAGGAACAAGTTCGTATTCTTCTGAAATCCGAGTGTTAATAGCTCGAAGTTGCTCGACAATCTAATGGGTCGATAGCAGTTTGCACTGTATACCTTTTTTTCCAATCTGATGCAGCAGTGCTTAATTTGGAAGTTTTGAGTGCGAGCGATCAATTAGTTTTCGTTGTTTGATTTTGTGTTTATTTCATTTTTAGTAATGAATGATTGGGTTTCTCTTTGTGGAAGTTTGGGGAAGGATTAGAGATGGCGGGACTTTGGAATGTTCTTGGCTTAATGGATTTGATTGCAAAGTGTTTGTGAAAATGCCTGTTTGAGCTCAAGAAAGATGAATTCTAATTTTTTGCCTTTGATATTATTGTGTTTGTTCTTTGAGCATTTGGGGCTTTTAGCTTTGTGATTCTGTTTGATAATCAATGCGACGAAATTGAAGTTGATCCTTTTTTGTTTCTTTGTATGATATGGTCTCTTACCTTGTTTGTCACACTATTATCTTCAGGTTGAAGACAATTGCTTCCGATGCTGGATTGTTTTGGGCTTTCTATCTAGTTTTCCTCTTGTTGTGTTGTGATTTCAAATGATAACAGTTCCGATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTATCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGATGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCCACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCGACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTATTGAGAACAAGGACTTGGCTCGCCGCGTGACAGTTATAATCTTTGACATTGAAACGACAGGTTTTAGTAGAAACAAAGATAGAATAGTGGAAATGGCATTTCGAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTCCAAATTCAGATCTACATGGCATTACAAACCGTATGGTCAGTCGACCCGAAGTTCCGAGGTATCGAAATGCTCGATGTAGTCGCATTCCTCTATTATTTACCCTCTCTGTTCAGTGCTAGTTAAATATATTGACTCCAATTTGAATCTTGGGTTAGGTTTATGCTAGGTTCCCCCATTCAGTTTTCCTAAGTAATTGACTTTCATTTTTATCTACTGTGATATTTGCAGAGTGGATCAATCAATATATAAACGTTTCTTTTCTATGTTCATGTAGGATGGAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTTATGTATTATTGGTAGCTCATAATGCTCGTGCCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTGAAAATTGGCTATTTTTCGATAACATGATTTTAGCCCGTCAGTTGATGAAATCCTCAGGTTAGTTTTTTCCCTTTGCTCATATTTTCTTGCTGGTTTGTTTTGTGATAGAAAGATTAATGTGATGTAATGTAATATCGTGATCAGATTCGAAGATTACGAGAATGTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGAGCAATGTCGGACGTGATGTCGTTGTCGCAGATTCTTCAAAGGCTTACTTTTGACCTCAAGCTAGGCATCTCGGATCTCGTCGAAAGATCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAGTGGCTTAAGTTAAATGCACAAATCAATCTTAACATAGTGATCAAGCTGTGCTTTTGTTGTAATGTTATTTATTAATTGTGATGTAACTTCAGTTCATTCTTATCATCAGAATCTTGGGCCATTTTATTTATGGTCAGCAGATTTGTCTTGATGTCCATATTCTCCAAAAACTAGTGGCTGAATTGGAATATCATGACAAATTAAATCTATTTTTGTATGCTTGTTTTGAATGGATTTTGAATGAAACAAGCATTGATAGAGGAGAGTTGTTTATGAATATAA

mRNA sequence

CCGATTTCCATGTCTCCGAAAAGCACGTCTCCATTTTCTTCTCCACGATTTACAGCCTCTCTCTCTCTCTCTCTCAACTTACTTTCTTCTGTTAGGTTCTCAAATGCAGCATAATCCTTCCTCTGATTTCCTTTCAGGTTGAAGACAATTGCTTCCGATGCTGGATTGTTTTGGGCTTTCTATCTAGTTTTCCTCTTGTTGTGTTGTGATTTCAAATGATAACAGTTCCGATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTATCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGATGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCCACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCGACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTATTGAGAACAAGGACTTGGCTCGCCGCGTGACAGTTATAATCTTTGACATTGAAACGACAGGTTTTAGTAGAAACAAAGATAGAATAGTGGAAATGGCATTTCGAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTCCAAATTCAGATCTACATGGCATTACAAACCGTATGGTCAGTCGACCCGAAGTTCCGAGGATGGAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTTATGTATTATTGGTAGCTCATAATGCTCGTGCCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTGAAAATTGGCTATTTTTCGATAACATGATTTTAGCCCGTCAGTTGATGAAATCCTCAGATTCGAAGATTACGAGAATGTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGAGCAATGTCGGACGTGATGTCGTTGTCGCAGATTCTTCAAAGGCTTACTTTTGACCTCAAGCTAGGCATCTCGGATCTCGTCGAAAGATCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAGTGGCTTAAGTTAAATGCACAAATCAATCTTAACATAGTGATCAAGCTGTGCTTTTGTTGTAATGTTATTTATTAATTGTGATGTAACTTCAGTTCATTCTTATCATCAGAATCTTGGGCCATTTTATTTATGGTCAGCAGATTTGTCTTGATGTCCATATTCTCCAAAAACTAGTGGCTGAATTGGAATATCATGACAAATTAAATCTATTTTTGTATGCTTGTTTTGAATGGATTTTGAATGAAACAAGCATTGATAGAGGAGAGTTGTTTATGAATATAA

Coding sequence (CDS)

ATGATAACAGTTCCGATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTATCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGATGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCCACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCGACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTATTGAGAACAAGGACTTGGCTCGCCGCGTGACAGTTATAATCTTTGACATTGAAACGACAGGTTTTAGTAGAAACAAAGATAGAATAGTGGAAATGGCATTTCGAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTCCAAATTCAGATCTACATGGCATTACAAACCGTATGGTCAGTCGACCCGAAGTTCCGAGGATGGAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTTATGTATTATTGGTAGCTCATAATGCTCGTGCCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTGAAAATTGGCTATTTTTCGATAACATGATTTTAGCCCGTCAGTTGATGAAATCCTCAGATTCGAAGATTACGAGAATGTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGAGCAATGTCGGACGTGATGTCGTTGTCGCAGATTCTTCAAAGGCTTACTTTTGACCTCAAGCTAGGCATCTCGGATCTCGTCGAAAGATCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAG

Protein sequence

MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSDLHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRLTFDLKLGISDLVERSFTASDLINKKKN
Homology
BLAST of Lcy03g001310 vs. ExPASy Swiss-Prot
Match: Q682U6 (Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DPD1 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-66
Identity = 147/323 (45.51%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  ++ DG+  RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSV-DGK-ARWIRRNV 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECY-IPNSDLHGI 185
           DL++ +TVI+ D+ETTG  R  +RI+E+A +D++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDI 245
            N MV RPEVPRMEELIPI L++V+SRQKP GYV+LVAHN ++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PENWLFFDNMILARQLMKSSDSKI-TRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY +   G AHRA+SDV+ LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFDLKLGISDLVERSFTASDL 327
           +LT DLKL +SDLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lcy03g001310 vs. ExPASy Swiss-Prot
Match: Q895K2 (DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=polC PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.2e-14
Identity = 65/201 (32.34%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 134 IIFDIETTGFSRNKDRIVEM-AFRDLSGGENSTFQTLINPECYIPNS--DLHGITNRMVS 193
           ++FDIETTGFS   D+I+E+ A +   G     + T +NPE  IP    +L GI + MV 
Sbjct: 414 VVFDIETTGFSNKNDKIIEIGAVKIKEGKVVDKYSTFVNPERIIPEKIIELTGIHDYMVK 473

Query: 194 RPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIPENWLF 253
             + P++EE++P  + F++         +LVAHNA  FDV F+ K       +  +  + 
Sbjct: 474 --DAPKIEEVLPKFIDFIEDS-------ILVAHNAN-FDVSFIKK-------NCKDFGIH 533

Query: 254 FDNMIL-ARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRLTFDL 313
           F+N IL    L K    ++ R  L  + ++  IPL+ + HRA+ D  +   IL +   DL
Sbjct: 534 FNNPILDTIPLCKFLYPELKRYKLNVVAKHLGIPLL-NHHRAVEDAKTTGDILLKAFEDL 593

Query: 314 K----LGISDLVERSFTASDL 327
           K    + ++ L E  F   D+
Sbjct: 594 KDKEIMNLNMLNEEYFKNQDI 596

BLAST of Lcy03g001310 vs. ExPASy Swiss-Prot
Match: Q8RA32 (DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=polC PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.3e-12
Identity = 57/189 (30.16%), Postives = 97/189 (51.32%), Query Frame = 0

Query: 134 IIFDIETTGFSRNKDRIVEMAFRDLSGGE-NSTFQTLINPECYIPN--SDLHGITNRMVS 193
           ++FDIETTG S   D I+E+    +   +   TF+T +NP+  I +  + L GI   MV 
Sbjct: 389 VVFDIETTGLSNINDEIIEIGAVKIKNKKIVDTFETFVNPQIPISSFITKLTGIDESMVK 448

Query: 194 RPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIPENWLF 253
             + P +EE++P  L+F K         +LVAHNA  FDV F+  +  +  + +    L 
Sbjct: 449 --DAPLIEEVLPKFLEFAKG-------AVLVAHNAN-FDVSFIKSKAKKLGLTVENTVL- 508

Query: 254 FDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRLTFDL- 313
            D + L+R L +     +    L  L E++++ L+   HRA+ D  + ++I  ++   L 
Sbjct: 509 -DTLELSRHLYQD----LKNYKLDTLAEFFEVKLL-HHHRAVEDAKATAEIFIKMLEKLQ 560

Query: 314 KLGISDLVE 319
           ++GI  + E
Sbjct: 569 EIGIKSVSE 560

BLAST of Lcy03g001310 vs. ExPASy Swiss-Prot
Match: Q8XJR3 (DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=polC PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.4e-11
Identity = 57/173 (32.95%), Postives = 91/173 (52.60%), Query Frame = 0

Query: 134 IIFDIETTGFSRNKDRIVEMAFRDLSGGE-NSTFQTLINPECYIPN--SDLHGITNRMVS 193
           ++FDIETTGFS   D+I+E+    L  GE   +F T ++P+  IP   ++L  IT  MV+
Sbjct: 436 VVFDIETTGFSSKNDKIIEIGAVKLKDGEIVDSFSTFVDPKVNIPYKITELTSITQNMVN 495

Query: 194 RPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIPENWLF 253
               P ++E++P  ++FV       G  +LVAHNA AFDV F+ K        +      
Sbjct: 496 GQ--PTIDEVLPKFMEFV-------GNSVLVAHNA-AFDVGFIKKNLMDMGKTLKNP--V 555

Query: 254 FDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 304
            D + LAR L       + ++ L  + ++  I L  + HRA+ D  + ++IL+
Sbjct: 556 MDTVPLARYLYPD----LKKVKLNLVAKHLGISL-ENHHRAVDDAKATAEILK 591

BLAST of Lcy03g001310 vs. ExPASy Swiss-Prot
Match: Q8EQU6 (DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=polC PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.3e-09
Identity = 57/177 (32.20%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 132 TVIIFDIETTGFSRNKDRIVEMAFRDLSGGE-NSTFQTLINPECYIPNS--DLHGITNRM 191
           T ++FD+ETTG S   D I+E+A   + GGE    F++  NP   +  +  DL GIT+ M
Sbjct: 413 TFVVFDVETTGLSAVYDTIIELAAVKIKGGEIVDRFESFANPHHALSQTTIDLTGITDDM 472

Query: 192 VSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIPENW 251
           V+  + P + +++       K      G  +LVAHNA +FD+ FL + F R   +   N 
Sbjct: 473 VN--DAPEVADVL-------KDFHTWMGNDILVAHNA-SFDMGFLNQGFQRIDYEKASNP 532

Query: 252 LFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRL 306
           +  D + LAR L+     ++    L  L ++ DI L    HRA+ D  +   +L +L
Sbjct: 533 V-VDTLELARFLL----PELRNHRLNTLCKHLDIELT-QHHRAIYDAEATGYLLWKL 573

BLAST of Lcy03g001310 vs. ExPASy TrEMBL
Match: A0A6J1HDU6 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461962 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.8e-158
Identity = 282/332 (84.94%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT PMCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFR YNLE GQ+KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           I+++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQF+KSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKIT++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKLGI DL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lcy03g001310 vs. ExPASy TrEMBL
Match: A0A6J1HUD7 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467897 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 284/331 (85.80%), Postives = 310/331 (93.66%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT  MCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFRIYNLE GQ+KRW
Sbjct: 1   MITASMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRKAKT+PS KPS+IRNEILEESVSTSCTVSINK+EK  TQKLQ C+IQ+T
Sbjct: 61  TRKSMTTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           II++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 IIKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQFVKSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKIT++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKK 332
           ILQRLTFDLKLGI DL+ERSFT SDLINKKK
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Lcy03g001310 vs. ExPASy TrEMBL
Match: A0A6J1C1V8 (exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007515 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.7e-157
Identity = 282/332 (84.94%), Postives = 303/332 (91.27%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MITVPMCFSILRVPKFGVHTL+N WR NFHPLNRNYGHSSWY QL FRIYNLE GQNKRW
Sbjct: 1   MITVPMCFSILRVPKFGVHTLANFWRTNFHPLNRNYGHSSWYDQLCFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRK K +PS KPSDIRNEILE SVSTSCTVSINKTEKSETQKLQYCDIQQT
Sbjct: 61  TRKSITTKAGRKVKNNPSSKPSDIRNEILEGSVSTSCTVSINKTEKSETQKLQYCDIQQT 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
             + K+L R VTVI FDIETTGFSR KDRIVE+AF+DLSGGENSTFQTLINPECYIPNS 
Sbjct: 121 FTKKKELTRPVTVIAFDIETTGFSRKKDRIVEIAFQDLSGGENSTFQTLINPECYIPNSL 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM++LIPILLQFV SRQKPDGY+LLVAHNAR FDVPFLL EFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKDLIPILLQFVLSRQKPDGYILLVAHNARTFDVPFLLNEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           S DIP++WLF DNMILARQLM SSDSK++++SLQALREYY IPLVGSAHRAMSDVMSLS 
Sbjct: 241 SFDIPQDWLFLDNMILARQLMISSDSKLSKISLQALREYYSIPLVGSAHRAMSDVMSLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKL I DL+ERSFTASDLI K K+
Sbjct: 301 ILQRLTFDLKLEIPDLIERSFTASDLIKKNKD 332

BLAST of Lcy03g001310 vs. ExPASy TrEMBL
Match: A0A0A0LVY3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.7e-140
Identity = 255/326 (78.22%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI R PK+GV  L+NLWRENFHPLNRN+GH+ WY QL FRIYNLE G+NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           TTKAG KAKT+PS KP++IRNEIL+ES+  SCTV+INK+EKSETQKLQYCDIQ TII++K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSDLHGIT 185
           + A  VTVI FDIETTGFSRN DRIVE+AF+DLSGGENSTFQTLINP+CYI NS++HGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 186 NRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIP 245
           NRMV  P VPRM+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR SVDIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 246 ENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRL 305
            NWLFFD M LARQLMK SDSKIT+++LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 306 TFDLKLGISDLVERSFTASDLINKKK 332
           TFDLKL ISDLVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Lcy03g001310 vs. ExPASy TrEMBL
Match: A0A0N9LRH7 (Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.7e-140
Identity = 255/326 (78.22%), Postives = 283/326 (86.81%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI R PK+GV  L+NLWRENFHPLNRN+GH+ WY QL FRIYNLE G+NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           TTKAG KAKT+PS KP++IRNEIL+ESV  SCTV+INK+EKSETQKLQYCDIQ TII++K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSDLHGIT 185
           + A  VTVI FDIETTGFSRN DRIVE+AF+DLSGGENSTFQTLINP+CYI NS++HGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 186 NRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDIP 245
           NRMV  P VP M+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR SVDIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 246 ENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQRL 305
            NWLFFD M LARQLMK SDSKIT+++LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 306 TFDLKLGISDLVERSFTASDLINKKK 332
           TFDLKL ISDLVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Lcy03g001310 vs. NCBI nr
Match: KAG6590475.1 (Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024006.1 Exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568.5 bits (1464), Expect = 3.7e-158
Identity = 283/332 (85.24%), Postives = 312/332 (93.98%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT PMCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFRIYNLE GQ+KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           I+++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQF+KSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKIT++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKLGI DL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lcy03g001310 vs. NCBI nr
Match: XP_022961373.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 282/332 (84.94%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT PMCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFR YNLE GQ+KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           I+++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQF+KSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKIT++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKLGI DL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lcy03g001310 vs. NCBI nr
Match: XP_023515555.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.2 bits (1458), Expect = 1.8e-157
Identity = 282/332 (84.94%), Postives = 310/332 (93.37%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT PMCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFRIYNLE GQNKRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT PS KPS+IRNEILEESVSTSCT+SINK+EK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTISINKSEKNGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           I ++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 ITKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQF+KSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKI ++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKIKKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKLGI DL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lcy03g001310 vs. NCBI nr
Match: XP_022968752.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 565.1 bits (1455), Expect = 4.1e-157
Identity = 284/331 (85.80%), Postives = 310/331 (93.66%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MIT  MCFSILRVPKFGV TL+NLWRENFHPLNRNYGHSSWY QLGFRIYNLE GQ+KRW
Sbjct: 1   MITASMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRKAKT+PS KPS+IRNEILEESVSTSCTVSINK+EK  TQKLQ C+IQ+T
Sbjct: 61  TRKSMTTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQET 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
           II++K+LAR VTVI FDIETTGFSR  DRIVE+A +DLSGGENSTFQTLINPECYIPN+ 
Sbjct: 121 IIKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM+ELIPILLQFVKSRQKPDGYVLLVAHNAR FDVPFLLKEFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           SVDIP NWLFFDN++LARQLMKS+DSKIT++SLQALREYY+IPLVGSAHRAMSDV +LS 
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKK 332
           ILQRLTFDLKLGI DL+ERSFT SDLINKKK
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Lcy03g001310 vs. NCBI nr
Match: XP_022135594.1 (exonuclease DPD1, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 282/332 (84.94%), Postives = 303/332 (91.27%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRW 60
           MITVPMCFSILRVPKFGVHTL+N WR NFHPLNRNYGHSSWY QL FRIYNLE GQNKRW
Sbjct: 1   MITVPMCFSILRVPKFGVHTLANFWRTNFHPLNRNYGHSSWYDQLCFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRK K +PS KPSDIRNEILE SVSTSCTVSINKTEKSETQKLQYCDIQQT
Sbjct: 61  TRKSITTKAGRKVKNNPSSKPSDIRNEILEGSVSTSCTVSINKTEKSETQKLQYCDIQQT 120

Query: 121 IIENKDLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECYIPNSD 180
             + K+L R VTVI FDIETTGFSR KDRIVE+AF+DLSGGENSTFQTLINPECYIPNS 
Sbjct: 121 FTKKKELTRPVTVIAFDIETTGFSRKKDRIVEIAFQDLSGGENSTFQTLINPECYIPNSL 180

Query: 181 LHGITNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRC 240
           +HGIT+RMVSRP+VPRM++LIPILLQFV SRQKPDGY+LLVAHNAR FDVPFLL EFSRC
Sbjct: 181 VHGITSRMVSRPDVPRMKDLIPILLQFVLSRQKPDGYILLVAHNARTFDVPFLLNEFSRC 240

Query: 241 SVDIPENWLFFDNMILARQLMKSSDSKITRMSLQALREYYDIPLVGSAHRAMSDVMSLSQ 300
           S DIP++WLF DNMILARQLM SSDSK++++SLQALREYY IPLVGSAHRAMSDVMSLS 
Sbjct: 241 SFDIPQDWLFLDNMILARQLMISSDSKLSKISLQALREYYSIPLVGSAHRAMSDVMSLSL 300

Query: 301 ILQRLTFDLKLGISDLVERSFTASDLINKKKN 333
           ILQRLTFDLKL I DL+ERSFTASDLI K K+
Sbjct: 301 ILQRLTFDLKLEIPDLIERSFTASDLIKKNKD 332

BLAST of Lcy03g001310 vs. TAIR 10
Match: AT5G26940.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 147/323 (45.51%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  ++ DG+  RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSV-DGK-ARWIRRNV 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECY-IPNSDLHGI 185
           DL++ +TVI+ D+ETTG  R  +RI+E+A +D++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDI 245
            N MV RPEVPRMEELIPI L++V+SRQKP GYV+LVAHN ++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PENWLFFDNMILARQLMKSSDSKI-TRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY +   G AHRA+SDV+ LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFDLKLGISDLVERSFTASDL 327
           +LT DLKL +SDLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lcy03g001310 vs. TAIR 10
Match: AT5G26940.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 147/323 (45.51%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  ++ DG+  RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSV-DGK-ARWIRRNV 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECY-IPNSDLHGI 185
           DL++ +TVI+ D+ETTG  R  +RI+E+A +D++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDI 245
            N MV RPEVPRMEELIPI L++V+SRQKP GYV+LVAHN ++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PENWLFFDNMILARQLMKSSDSKI-TRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY +   G AHRA+SDV+ LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFDLKLGISDLVERSFTASDL 327
           +LT DLKL +SDLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lcy03g001310 vs. TAIR 10
Match: AT5G26940.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 147/323 (45.51%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  ++ DG+  RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSV-DGK-ARWIRRNV 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECY-IPNSDLHGI 185
           DL++ +TVI+ D+ETTG  R  +RI+E+A +D++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDI 245
            N MV RPEVPRMEELIPI L++V+SRQKP GYV+LVAHN ++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PENWLFFDNMILARQLMKSSDSKI-TRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY +   G AHRA+SDV+ LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFDLKLGISDLVERSFTASDL 327
           +LT DLKL +SDLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lcy03g001310 vs. TAIR 10
Match: AT5G26940.4 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 147/323 (45.51%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLSNLWRENFHPLNRNYGHSSWYGQLGFRIYNLEDGQNKRWTRKSV 65
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  ++ DG+  RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSV-DGK-ARWIRRNV 60

Query: 66  TTKAGRKAKTHPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIIENK 125
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 126 DLARRVTVIIFDIETTGFSRNKDRIVEMAFRDLSGGENSTFQTLINPECY-IPNSDLHGI 185
           DL++ +TVI+ D+ETTG  R  +RI+E+A +D++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TNRMVSRPEVPRMEELIPILLQFVKSRQKPDGYVLLVAHNARAFDVPFLLKEFSRCSVDI 245
            N MV RPEVPRMEELIPI L++V+SRQKP GYV+LVAHN ++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PENWLFFDNMILARQLMKSSDSKI-TRMSLQALREYYDIPLVGSAHRAMSDVMSLSQILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY +   G AHRA+SDV+ LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFDLKLGISDLVERSFTASDL 327
           +LT DLKL +SDLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q682U65.9e-6645.51Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q895K25.2e-1432.34DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) ... [more]
Q8RA328.3e-1230.16DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongens... [more]
Q8XJR31.4e-1132.95DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=... [more]
Q8EQU62.3e-0932.20DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP... [more]
Match NameE-valueIdentityDescription
A0A6J1HDU66.8e-15884.94exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1HUD72.0e-15785.80exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1C1V85.7e-15784.94exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0LVY31.7e-14078.22Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080... [more]
A0A0N9LRH73.7e-14078.22Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6590475.13.7e-15885.24Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma s... [more]
XP_022961373.11.4e-15784.94exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374... [more]
XP_023515555.11.8e-15784.94exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022968752.14.1e-15785.80exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1... [more]
XP_022135594.11.2e-15684.94exonuclease DPD1, chloroplastic/mitochondrial [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G26940.14.2e-6745.51Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.24.2e-6745.51Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.34.2e-6745.51Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.44.2e-6745.51Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 132..311
e-value: 5.6E-29
score: 112.3
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 134..300
e-value: 6.5E-22
score: 78.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 131..311
e-value: 1.4E-44
score: 154.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..82
NoneNo IPR availablePANTHERPTHR13058:SF23POLYMERASE III POLC-TYPE, PUTATIVE ISOFORM 1-RELATEDcoord: 3..329
NoneNo IPR availableCDDcd06127DEDDhcoord: 134..303
e-value: 6.79758E-40
score: 135.891
IPR040393Three-prime repair exonuclease 1/2PANTHERPTHR13058THREE PRIME REPAIR EXONUCLEASE 1, 2coord: 3..329
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 132..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g001310.1Lcy03g001310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000738 DNA catabolic process, exonucleolytic
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008408 3'-5' exonuclease activity