Lcy03g001030 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g001030
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	exopolygalacturonase-like
Location	Chr03: 3438107 .. 3440427 (+)
RNA-Seq Expression	Lcy03g001030
Synteny	Lcy03g001030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATGAAAACAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGCTGTGAAAAGGTGGCTGCCATTTTCGACGACGTCACCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACAGTAAATCAGCAGCTTCCTGCAGCCGCTCCGCAGGCTCTTGGTATCGGAACTCTGACAGGCCGCGGCGGTAGTGTTACAGTAAATGATGTTAAGGCTGTTGTTGATCGGAATAGGAAGGGTGGTGCAATTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATGATGCACAAGTGATGATAATTAACTAACGTCAATGAATCACAAAAGTATAAACTTTTAATTTGAGTAATTACCTTTGAACTTATAAAATTATTGAATTTCAACAATTATCATCTAAATATACAAAACCATTGACATTACATCCCTACTGTTTGTAACTCCATTTGCTATTGGATTTAATTGCAATAATTTTATAAATTTGAACAAGTATAATTGCAACAATATTATTTAAATAATTTTTTTAAAAAATACTAATTATGAAGATTATAGGGATTTTTTTTAATTCAACAATATGTTTGGGTGAGGATCTTCCGAACAAGTTTATAATATCCTAACCAGTTGCAAAAAAATTCCAAGATTTAAACTTATATGACTCAAAAGTTTAACGGTAACAAAAATTTACCAACTTAAAGATAAGTCATCTGGTAAAAACAATCCCTACCTTTCTAATTTAACTTGCATCTTTTTTCTACTTTTCTAAAAAAAAAAACTTTGATTTTCCATGTGGCAGGCATTCATGACGACATGGATTGAAGCATGTCGGAACACAGCCGGTCCGGCGAAGTTTTTGATCCCACCAGGCACATTTCTAGCGGGTCCGGTGACTTTTGCTGGTCCGTGCCAAAGCTTGCCGATCACCATCGAAATTAAGGGAACTGTAAAAGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAAGGTATCACCGGCCTTATCCTCACCGGCTCCGGCGTCTTCGACGGGCAAGGCGCCTCTGCCTGGTCCTACAACGATTGCAAGAAAAACATTAACTGCCAGAGTCTTCCAATCGTAACTATAATTCTCCCTAACTCAATCATCCCATATTTAATTATAAATTAGAAGGCTTGGAACTTTTAAAGTTATGTCTAATTAATATGAAGTAAGAAATTCACTATTAAAATCCAGTCCTTTGCTATTAAAAAATTGTTTCAAGGACTAAATTTATAATTTGTTCTAATTATTATTGTGATTAATTAATTGTTTGTTTGTTTTTTTTTTTTTGCAGTCAATTAAATTCTCAAAATTGAACCACACAATAGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAATATGCACATTATAGCCCCTGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCAAAATTGGTCACTATTTCTAACAGCGTCATGGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTGAGAAAATCACTGTCACTAATATCACTTGCGGTCCTGGACATGGCATTAGGTAATTATATCTTTTCGAAAAAAAAATTAGATTAAATAGACACAAATCTTTTAACTTTGTAATTTTCATAGAAAAAGAAAAGCATCTCAAAGTTGTTTATTTTCTTATAGTGTGGGCAGCTTGGGCAAGTATCCGAAAGAGAAGAGTGTCTTTGGCGTTCTAGTGCAAAACTGCACCATTTTTAACGCCACCAACGGCGCTAGAATCAAGACATGGGCTGGCACCGTGTCAGGGTCGGCCACGGGAATAATCTTCGATGACATTGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACCTATGGCACAAAAAAGAACAAGGTAACAACAACAACAAAACAAACTAATCTACATTCTTCTTAGAACATTTATCACAAACCATCAACTTAAGCTTTTGAGTTTAGTAGTTATTTTACGCTCTACAATTTGAGCATGCAGGCTTCAAAGTGGAAGATCAGCGATGTTCACTTCAAGAACATCCGTGGGACATCGGTGACGAACGTGGCGGTGTTGTTGGAGTGCAGCGCATTGTTTCCATGCGAGGGGGTGGAGCTGAGGGATATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCGTCATGTTTGAATGCAAAAATTAAACATCATGGGATGCAGAATCCATCGCCTTGTGTTGTATGA

mRNA sequence

ATGACAATGAAAACAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGCTGTGAAAAGGTGGCTGCCATTTTCGACGACGTCACCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACAGTAAATCAGCAGCTTCCTGCAGCCGCTCCGCAGGCTCTTGGTATCGGAACTCTGACAGGCCGCGGCGGTAGTGTTACAGTAAATGATGTTAAGGCTGTTGTTGATCGGAATAGGAAGGGTGGTGCAATTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATGATGCACAAGCATTCATGACGACATGGATTGAAGCATGTCGGAACACAGCCGGTCCGGCGAAGTTTTTGATCCCACCAGGCACATTTCTAGCGGGTCCGGTGACTTTTGCTGGTCCGTGCCAAAGCTTGCCGATCACCATCGAAATTAAGGGAACTGTAAAAGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAAGGTATCACCGGCCTTATCCTCACCGGCTCCGGCGTCTTCGACGGGCAAGGCGCCTCTGCCTGGTCCTACAACGATTGCAAGAAAAACATTAACTGCCAGAGTCTTCCAATCTCAATTAAATTCTCAAAATTGAACCACACAATAGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAATATGCACATTATAGCCCCTGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCAAAATTGGTCACTATTTCTAACAGCGTCATGGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTGAGAAAATCACTGTCACTAATATCACTTGCGGTCCTGGACATGGCATTAGTGTGGGCAGCTTGGGCAAGTATCCGAAAGAGAAGAGTGTCTTTGGCGTTCTAGTGCAAAACTGCACCATTTTTAACGCCACCAACGGCGCTAGAATCAAGACATGGGCTGGCACCGTGTCAGGGTCGGCCACGGGAATAATCTTCGATGACATTGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACCTATGGCACAAAAAAGAACAAGGCTTCAAAGTGGAAGATCAGCGATGTTCACTTCAAGAACATCCGTGGGACATCGGTGACGAACGTGGCGGTGTTGTTGGAGTGCAGCGCATTGTTTCCATGCGAGGGGGTGGAGCTGAGGGATATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCGTCATGTTTGAATGCAAAAATTAAACATCATGGGATGCAGAATCCATCGCCTTGTGTTGTATGA

Coding sequence (CDS)

ATGACAATGAAAACAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGCTGTGAAAAGGTGGCTGCCATTTTCGACGACGTCACCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACAGTAAATCAGCAGCTTCCTGCAGCCGCTCCGCAGGCTCTTGGTATCGGAACTCTGACAGGCCGCGGCGGTAGTGTTACAGTAAATGATGTTAAGGCTGTTGTTGATCGGAATAGGAAGGGTGGTGCAATTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATGATGCACAAGCATTCATGACGACATGGATTGAAGCATGTCGGAACACAGCCGGTCCGGCGAAGTTTTTGATCCCACCAGGCACATTTCTAGCGGGTCCGGTGACTTTTGCTGGTCCGTGCCAAAGCTTGCCGATCACCATCGAAATTAAGGGAACTGTAAAAGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAAGGTATCACCGGCCTTATCCTCACCGGCTCCGGCGTCTTCGACGGGCAAGGCGCCTCTGCCTGGTCCTACAACGATTGCAAGAAAAACATTAACTGCCAGAGTCTTCCAATCTCAATTAAATTCTCAAAATTGAACCACACAATAGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAATATGCACATTATAGCCCCTGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCAAAATTGGTCACTATTTCTAACAGCGTCATGGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTGAGAAAATCACTGTCACTAATATCACTTGCGGTCCTGGACATGGCATTAGTGTGGGCAGCTTGGGCAAGTATCCGAAAGAGAAGAGTGTCTTTGGCGTTCTAGTGCAAAACTGCACCATTTTTAACGCCACCAACGGCGCTAGAATCAAGACATGGGCTGGCACCGTGTCAGGGTCGGCCACGGGAATAATCTTCGATGACATTGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACCTATGGCACAAAAAAGAACAAGGCTTCAAAGTGGAAGATCAGCGATGTTCACTTCAAGAACATCCGTGGGACATCGGTGACGAACGTGGCGGTGTTGTTGGAGTGCAGCGCATTGTTTCCATGCGAGGGGGTGGAGCTGAGGGATATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCGTCATGTTTGAATGCAAAAATTAAACATCATGGGATGCAGAATCCATCGCCTTGTGTTGTATGA

Protein sequence

MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQLPAAAPQALGIGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV

Homology

[Close]

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 6.6e-92
Identity = 164/347 (47.26%), Postives = 228/347 (65.71%), Query Frame = 0


Query: 82  NRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAG 141
           N     ++D+ K+GA  DG T+  +AF+ TWI+ C ++  PA  L+P GTFLAGPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVC-DSPVPATLLVPKGTFLAGPVIFAG 104

Query: 142 PCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCK 201
           PC+S  +T+ + GT+ ATT  S Y++PEWF  E +  L+LTG+G F G+G + W  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 202 KNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTD 261
           K + C   P S+KF  + +  ++GI+S+N+KAFH  +    N    N+ + AP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 262 GMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSV 321
           G+HLS +  V+I +S + TGDDCVS+G+G   +TV  + CGPGHG+SVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 322 FGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKA 381
            G+ V NCT+    NG RIKTW G+    A  I F++I+M +VK PIIIDQ YG++    
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD- 344

Query: 382 SKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGG 429
           S+  ISD+ FKNIRGT++T   V + CS   PC+GV + D+NL Y G
Sbjct: 345 SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVG 386

[Close]

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.2e-88
Identity = 168/370 (45.41%), Postives = 225/370 (60.81%), Query Frame = 0


Query: 91  VRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITI 150
           V K+GAKADGKTD ++ F+  W EAC +   P+  +IP GT+L   V   GPC++ PI I
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVT-PSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 151 EIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKKNINCQSLP 210
            ++GT++A  D S +  P W     +    + G G+FDGQG+ A+  N C+       LP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 211 ISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKL 270
           ++I+F  L + ++  ITS +SK FH +VF C N T   + I AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 271 VTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCT 330
           V I  S + TGDDC+SIG G + + +  ITCGPGHGIS+GSLGK+  E+ V G+ + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 331 IFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKI 390
           I N +NGARIKTW G   G+ + I F+DI M NV  PI+IDQ Y      KKN+ SK K+
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKL 331

Query: 391 SDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTIVSSCLNAKIK 450
           S++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN K  
Sbjct: 332 SNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAE----PATSQCLNVKPI 391

Query: 451 HHGMQNPSPC 457
             G  NP PC
Sbjct: 392 TSGKLNPIPC 395

[Close]

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.6e-88
Identity = 164/376 (43.62%), Postives = 230/376 (61.17%), Query Frame = 0


Query: 83  RKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGP 142
           +  G++F+V  YGAK  G  D +QA M  W  AC  + GP+  LIP G +  G V   GP
Sbjct: 4   QSSGSVFNVNDYGAKGAG--DISQAVMKAWKAACA-SQGPSTVLIPKGNYNMGEVAMQGP 63

Query: 143 CQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKK 202
           C+   I  +I G VKA  D S++ S  W S   I GL ++G+G  DGQG +AW+ N+C K
Sbjct: 64  CKGSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDK 123

Query: 203 NINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDG 262
           N NC+   ++++F  L H +V  ITSLNSK FH +V  C + T  ++ + APG S NTDG
Sbjct: 124 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 183

Query: 263 MHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVF 322
           +H+  SK VTI+N+ + TGDDC+SIG G + +T+T + CGPGHGIS+GSLG+Y  EK V 
Sbjct: 184 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 243

Query: 323 GVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKK 382
           G+ V+ CT     NG R+KTW  +  G+AT + F D+ M NV+ P+I+DQ Y       +
Sbjct: 244 GITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSR 303

Query: 383 NKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTIVS 442
              S+ K+S+++F NIRGTS   VAV++ CS   PC  +++ +INL+Y G         S
Sbjct: 304 QAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRG---AGGPATS 363

Query: 443 SCLNAKIKHHGMQNPS 455
           +C N K    G Q P+
Sbjct: 364 TCSNVKPTFSGKQVPA 373

[Close]

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 4.4e-88
Identity = 167/370 (45.14%), Postives = 225/370 (60.81%), Query Frame = 0


Query: 91  VRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITI 150
           V K+GAKADGKTD ++ F+  W EAC +   P+  +IP GT+L   V   GPC++ PI I
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVT-PSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 151 EIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKKNINCQSLP 210
            ++GT++A  D S +  P W     +    + G G+FDGQG+ A+  N C+       LP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 211 ISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKL 270
           ++I+F  + + ++  ITS +SK FH +VF C N T   + I AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 271 VTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCT 330
           V I  S + TGDDC+SIG G + + +  ITCGPGHGIS+GSLGK+  E+ V G+ + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 331 IFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKI 390
           I N +NGARIKTW G   G+ + I F+DI M NV  PI+IDQ Y      KKN+ SK K+
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKL 331

Query: 391 SDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTIVSSCLNAKIK 450
           S++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN K  
Sbjct: 332 SNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAE----PATSQCLNVKPI 391

Query: 451 HHGMQNPSPC 457
             G  NP PC
Sbjct: 392 TIGKLNPIPC 395

[Close]

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.4e-86
Identity = 172/390 (44.10%), Postives = 232/390 (59.49%), Query Frame = 0


Query: 73  NDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTF 132
           ND KA    +  GG+ FD+ K GA  +GKTD  +A    W  AC  T G    LIP G F
Sbjct: 30  NDAKA----SGPGGS-FDITKLGASGNGKTDSTKAVQEAWASACGGT-GKQTILIPKGDF 89

Query: 133 LAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSS-PEWFSIEGITGLILTGSGVFDGQG 192
           L GP+ F GPC+   +TI++ G + ATTD+S+Y     W  I  +  L++TG G  DGQG
Sbjct: 90  LVGPLNFTGPCKG-DVTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQG 149

Query: 193 ASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHI 252
            + WS N C K  +C+ LP S+    +N+  V GIT LNSK FH +++ C +    ++++
Sbjct: 150 PAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNV 209

Query: 253 IAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGS 312
            APG+SPNTDG+H+  S  VTI+N+V+G GDDC+SIG G  K+ +T +TCGPGHGIS+GS
Sbjct: 210 TAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGS 269

Query: 313 LGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSG-SATGIIFDDIVMYNVKYPIII 372
           LG+Y  EK V  + V++CT+    NG RIK +    S  +A+ I +++I M +  YPIII
Sbjct: 270 LGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIII 329

Query: 373 DQTYGTKK----NKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTY 432
           D  Y   K    N ASK  + DV FKNI GTS T  AV L C+A  PC GV + D+N+ Y
Sbjct: 330 DMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKY 389

Query: 433 GGNNLRNTTIVSSCLNAKIKHHGMQNPSPC 457
            G N +   +   C NAK    G      C
Sbjct: 390 SGTNNKTMAV---CKNAKGSAKGCLKELAC 409

[Close]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL
Match: A0A6J1JX85 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.0e-205
Identity = 358/459 (78.00%), Postives = 391/459 (85.19%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT   + SLFQ LLLV A QCC +VAA+FD VTG  NL+ GI ST NQQ  P AAP  LG
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQHRPPAAPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNT 120
           I T   R GSV +N  KA V     G A+FDV+KYGAKA+GK+DDAQAFMTTWI ACRNT
Sbjct: 61  IRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACRNT 120

Query: 121 AGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGL 180
            GPAKFLIP G FL GPVTFAGPC+S+PITIEI+GTVKATTDISEYSSP+W SIEGITGL
Sbjct: 121 TGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGITGL 180

Query: 181 ILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVF 240
           ILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTSVF
Sbjct: 181 ILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTSVF 240

Query: 241 NCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNI 300
            CYNFTATNM+I+APGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVTN+
Sbjct: 241 RCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVTNV 300

Query: 301 TCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDI 360
           TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA T+SGSA GI+FD+I
Sbjct: 301 TCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGSAVGIVFDNI 360

Query: 361 VMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVEL 420
           VM  VK PIIIDQTYGTK+ KASKWKISDVHFKNIRGTS TNVAVLLECS LFPCEGVEL
Sbjct: 361 VMRKVKNPIIIDQTYGTKEKKASKWKISDVHFKNIRGTSTTNVAVLLECSELFPCEGVEL 420

Query: 421 RDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           RDINL+YGG +L+NTT VSSCLNAKIK  G+QNP  CVV
Sbjct: 421 RDINLSYGGASLKNTTTVSSCLNAKIKTFGVQNPPACVV 459

[Close]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL
Match: A0A6J1GQZ3 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111456314 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.1e-202
Identity = 358/461 (77.66%), Postives = 389/461 (84.38%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT  T+ SLFQ LLLV A QCC +VAA+FD V    N + GI ST +QQ  P A P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPINGIVSTTSQQHRPPAPPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVVD--RNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACR 120
           IGTL    G V +N  KA VD   N  G A+FDV+KYGAKA+GK+DDAQAFMTTWI ACR
Sbjct: 61  IGTLANSHGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 NTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGIT 180
           NT GPAKFLIP GTFL GPV FAGPCQS PITIEI+GTVKATTDISEYSSP+W SIEGIT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTS 240
           GLILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVT 300
           VF CYNFTATNM+IIAPGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVT
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA T+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+V FKNIRGTS TNVAVLLECS LFPCEGV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVLFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           ELRDINL+YGG +L+NTTIVSSCLNAKI+  G+QNP  CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL
Match: A0A5A7U4W1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00950 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.1e-198
Identity = 351/452 (77.65%), Postives = 380/452 (84.07%), Query Frame = 0


Query: 9   LFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQL--PAAAPQALGIGTLTGR 68
           + QILLLVFA QC  KVAA  DDVTG  NL+     TVN+Q   PA AP+ LGIGTL   
Sbjct: 8   VIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLGIGTLPDI 67

Query: 69  GGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFL 128
           G   TVND++A +  N  GG++FDV K+GAKADG+TDDAQAFMTTWI ACRNT GPAKFL
Sbjct: 68  GS--TVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFL 127

Query: 129 IPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGV 188
           IP GT+L GPVTFAGPC+S PIT+E +GTVKATTDISEYSSPEWFSIE ITG ILTGSGV
Sbjct: 128 IPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFILTGSGV 187

Query: 189 FDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTA 248
           FDGQG + W YNDCKKN  CQ LPISIKFS+LNHTIVDG+TS+NS  FHTSVF CYNFTA
Sbjct: 188 FDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFYCYNFTA 247

Query: 249 TNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHG 308
           TNM IIAP NSPNTDGMHLSTSKLVTI+NSV+GTGDDCVSIG   E I VTN+TCGPGHG
Sbjct: 248 TNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVTCGPGHG 307

Query: 309 ISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKY 368
           +SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GIIF+DIVMYNVK 
Sbjct: 308 LSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIVMYNVKN 367

Query: 369 PIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTY 428
           PIIIDQTYGTKK K S WKISDVHFKNIRGTS TNVAVLLECS L PCEGVELRDINLTY
Sbjct: 368 PIIIDQTYGTKKKKVSNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINLTY 427

Query: 429 GGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           GG NLRNTTIVSSC NAKI   G+QNP PCVV
Sbjct: 428 GGTNLRNTTIVSSCSNAKIATFGVQNPPPCVV 457

[Close]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL
Match: A0A5A7TQ89 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36913G00010 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 7.1e-198
Identity = 350/452 (77.43%), Postives = 379/452 (83.85%), Query Frame = 0


Query: 9   LFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQL--PAAAPQALGIGTLTGR 68
           + QILLLVFA QC  K AA  DDVTG  NL+     TVN+Q   PA AP+ LGIGTL   
Sbjct: 8   VIQILLLVFASQCSAKDAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLGIGTLPDI 67

Query: 69  GGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFL 128
           G   TVND++A +  N  GG++FDV K+GAKADG+TDDAQAFMTTWI ACRNT GPAKFL
Sbjct: 68  GS--TVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVGPAKFL 127

Query: 129 IPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGV 188
           IP GT+L GPVTFAGPC+S PIT+E +GTVKATTDISEYSSPEWFSIE ITG ILTGSGV
Sbjct: 128 IPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFILTGSGV 187

Query: 189 FDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTA 248
           FDGQG + W YNDCKKN  CQ LPISIKFS+LNHTIVDG+TS+NS  FHTSVF CYNFTA
Sbjct: 188 FDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFYCYNFTA 247

Query: 249 TNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHG 308
           TNM IIAP NSPNTDGMHLSTSKLVTI+NSV+GTGDDCVSIG   E I VTN+TCGPGHG
Sbjct: 248 TNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVTCGPGHG 307

Query: 309 ISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKY 368
           +SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GIIF+DIVMYNVK 
Sbjct: 308 LSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIVMYNVKN 367

Query: 369 PIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTY 428
           PIIIDQTYGTKK K S WKISDVHFKNIRGTS TNVAVLLECS L PCEGVELRDINLTY
Sbjct: 368 PIIIDQTYGTKKKKVSNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELRDINLTY 427

Query: 429 GGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           GG NLRNTTIVSSC NAKI   G+QNP PCVV
Sbjct: 428 GGTNLRNTTIVSSCSNAKIATFGVQNPPPCVV 457

[Close]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL
Match: A0A1S3BRD3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.9e-190
Identity = 337/460 (73.26%), Postives = 382/460 (83.04%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQLPA--AAPQAL 60
           MT+     LFQILL VFAWQCC+KV+AI +D   I NL   I ST+NQQLP+  AAP  L
Sbjct: 1   MTITRDIGLFQILLWVFAWQCCDKVSAITND---IANLNYEIVSTINQQLPSPVAAPGPL 60

Query: 61  GIGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRN 120
           GI TL      V  +D+    D N  GG++F V K+GAKADGKTDDAQAF+TTWIEACRN
Sbjct: 61  GIETLPDLDNMVN-DDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRN 120

Query: 121 TAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITG 180
           T GPAK LIP GT+L GPVT AGPC+S PIT+E +GTVKATTDIS+YSSPEWFSIE ITG
Sbjct: 121 TVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITG 180

Query: 181 LILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSV 240
            ILTGSGVFDGQG +AW YNDCK N  CQ LPISIKFS+LN TIVD +TSLNSK FH S+
Sbjct: 181 FILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSL 240

Query: 241 FNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTN 300
           F+CYNFTATN++IIAP +SPNTDG+HLSTSKLV I+NS++GTGDDCVSIG   EKITVTN
Sbjct: 241 FSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTN 300

Query: 301 ITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDD 360
           +TCGPGHG+SVGSLGKYP+EK V+ VLV+NCTIFNATNGARIKT+A  +SGSA+GIIF+D
Sbjct: 301 VTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFED 360

Query: 361 IVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVE 420
           IVMYNVKYPIIIDQTY T +NK SKWK+SDVHFKNIRGTS TNVAVLL+CS L PCEGVE
Sbjct: 361 IVMYNVKYPIIIDQTYDTNENKESKWKVSDVHFKNIRGTSATNVAVLLDCSKLLPCEGVE 420

Query: 421 LRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           LRDI+LTYGG +L+NTTIVSSC NAKI   G+QNP PC V
Sbjct: 421 LRDIDLTYGGTDLKNTTIVSSCSNAKITTFGVQNPPPCDV 456

[Close]

BLAST of Lcy03g001030 vs. NCBI nr
Match: XP_038896336.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 735.7 bits (1898), Expect = 2.4e-208
Identity = 365/460 (79.35%), Postives = 397/460 (86.30%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQL--PAAAPQAL 60
           MTM TS SLFQILLL FAWQCC +VAAIFDD+TG  NLV G+ STVNQ L  PAAAP  L
Sbjct: 1   MTM-TSPSLFQILLLAFAWQCCAEVAAIFDDITGTANLVNGVVSTVNQPLLHPAAAPLPL 60

Query: 61  GIGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRN 120
           GIGTL   GG  TVND+KA VD N  GG++FDV K+GAK DGKTDDAQAFMTTWIEACRN
Sbjct: 61  GIGTLPDLGG--TVNDIKANVDLNGNGGSVFDVTKHGAKGDGKTDDAQAFMTTWIEACRN 120

Query: 121 TAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITG 180
             GPAKFLIP GTFL GPVTFAGPC+S PIT+E +GTVKATTDI++YSSPEWFSIE ITG
Sbjct: 121 AEGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEEITG 180

Query: 181 LILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSV 240
            ILTGSGVFDGQGA++W YNDCKKN  CQ LPISIKF+KLNHTIVDG+ SLNSK FHTS+
Sbjct: 181 FILTGSGVFDGQGAASWPYNDCKKNTKCQILPISIKFTKLNHTIVDGLASLNSKGFHTSI 240

Query: 241 FNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTN 300
           F CYNFTATNM+IIAPGNSPNTDGMH+STSKLVTI+NSV+GTGDDCVSIG   EKI VTN
Sbjct: 241 FYCYNFTATNMNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKIVVTN 300

Query: 301 ITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDD 360
           +TCGPGHG+SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GI F+D
Sbjct: 301 VTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPISGLASGITFED 360

Query: 361 IVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVE 420
           I+MYNVK PIIIDQTYGTK+ KAS WKISDVHFKNIRGTS TNVAV LECS L PCE VE
Sbjct: 361 IIMYNVKNPIIIDQTYGTKQKKASNWKISDVHFKNIRGTSTTNVAVSLECSELLPCEEVE 420

Query: 421 LRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           LRDINLTYGG NLRNTTI+SSC NAKI  +GMQNP  CVV
Sbjct: 421 LRDINLTYGGPNLRNTTILSSCSNAKIDSYGMQNPPACVV 457

[Close]

BLAST of Lcy03g001030 vs. NCBI nr
Match: XP_022991773.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.2e-204
Identity = 358/459 (78.00%), Postives = 391/459 (85.19%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT   + SLFQ LLLV A QCC +VAA+FD VTG  NL+ GI ST NQQ  P AAP  LG
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQHRPPAAPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNT 120
           I T   R GSV +N  KA V     G A+FDV+KYGAKA+GK+DDAQAFMTTWI ACRNT
Sbjct: 61  IRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACRNT 120

Query: 121 AGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGL 180
            GPAKFLIP G FL GPVTFAGPC+S+PITIEI+GTVKATTDISEYSSP+W SIEGITGL
Sbjct: 121 TGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGITGL 180

Query: 181 ILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVF 240
           ILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTSVF
Sbjct: 181 ILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTSVF 240

Query: 241 NCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNI 300
            CYNFTATNM+I+APGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVTN+
Sbjct: 241 RCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVTNV 300

Query: 301 TCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDI 360
           TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA T+SGSA GI+FD+I
Sbjct: 301 TCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGSAVGIVFDNI 360

Query: 361 VMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVEL 420
           VM  VK PIIIDQTYGTK+ KASKWKISDVHFKNIRGTS TNVAVLLECS LFPCEGVEL
Sbjct: 361 VMRKVKNPIIIDQTYGTKEKKASKWKISDVHFKNIRGTSTTNVAVLLECSELFPCEGVEL 420

Query: 421 RDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           RDINL+YGG +L+NTT VSSCLNAKIK  G+QNP  CVV
Sbjct: 421 RDINLSYGGASLKNTTTVSSCLNAKIKTFGVQNPPACVV 459

[Close]

BLAST of Lcy03g001030 vs. NCBI nr
Match: KAG7014351.1 (Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 721.8 bits (1862), Expect = 3.6e-204
Identity = 361/461 (78.31%), Postives = 390/461 (84.60%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT  T+ SLFQ LLLV A QCC +VAA+FD V    N + GI ST NQQ  P A P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVVD--RNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACR 120
           IGTL    G V +N  KA VD   N  G A+FDV+KYGAKA+GK+DDAQAFMTTWI ACR
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 NTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGIT 180
           NT GPAKFLIP GTFL GPV FAGPCQS PITIEI+GTVKATTDISEYSSP+W SIEGIT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTS 240
           GLILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVT 300
           VF CYNFTATNM+IIAPGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVT
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLGKY  EKSV  VLVQNCTIFNATNGARIKTWA T+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGKYKTEKSVLDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPCEGV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           ELRDINL+YGG +L+NTTIVSSCLNAKI+  G+QNP  CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Lcy03g001030 vs. NCBI nr
Match: XP_023549400.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.5 bits (1861), Expect = 4.7e-204
Identity = 362/461 (78.52%), Postives = 390/461 (84.60%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT  T+ SLFQ LLLV A QCC +VAA+FD VT   N + GI ST NQQ  P A P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVTVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVV--DRNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACR 120
           IGTL    G V +N  KA V    N  G A+FDV+KYGAKA+GKTDDAQAFMTTWI ACR
Sbjct: 61  IGTLANSRGRVAINGTKAAVYLKGNGNGNAVFDVKKYGAKANGKTDDAQAFMTTWIAACR 120

Query: 121 NTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGIT 180
           NT GPAKFLIP GTFL GPV FAGPC+S PITIEI+GTVKATTDISEYSSP+W SIEGIT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCKSWPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTS 240
           GLILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVT 300
           VF CYNFTATNM+IIAPGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVT
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFD 360
           NITCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA T+SGSA GIIFD
Sbjct: 301 NITCGPGHGISIGSLGRYKTEKSVSDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPCEGV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           ELRDINL+YGG + +NTTIVSSCLNAKIK  G+QNP PCVV
Sbjct: 421 ELRDINLSYGGASSKNTTIVSSCLNAKIKTFGVQNPPPCVV 461

[Close]

BLAST of Lcy03g001030 vs. NCBI nr
Match: KAG6575809.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719.2 bits (1855), Expect = 2.3e-203
Identity = 359/461 (77.87%), Postives = 389/461 (84.38%), Query Frame = 0


Query: 1   MTMKTSFSLFQILLLVFAWQCCEKVAAIFDDVTGIVNLVGGIASTVNQQ-LPAAAPQALG 60
           MT  T+ SLFQ LLLV A QCC +VAA+FD V    N + GI ST NQQ  P A P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGTLTGRGGSVTVNDVKAVVD--RNRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACR 120
           IGTL    G V +N  KA VD   N  G A+FDV+KYGAKA+GK+DDAQAFMTTWI ACR
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 NTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGIT 180
           NT GPAKFLIP GTFL GPV FAGPCQS PITIEI+GTVKATTDISEYSSP+W SIEGIT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTS 240
           GLILTGSGVFDGQGASAW YNDCK N NCQ LP SIKF++LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVT 300
           VF CYNFTATNM+IIAPGNSPNTDGMHLSTSKLVTIS+S +GTGDDCVSIG  CEKITVT
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNG RIKTWA T+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGVRIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPCEGV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPCVV 459
           ELRDINL+YGG +L+NTTIVSSCLNAKI+  G+QNP  CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Lcy03g001030 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 3.6e-93
Identity = 177/383 (46.21%), Postives = 245/383 (63.97%), Query Frame = 0


Query: 88  IFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLP 147
           + DVR +GA+A+   D  +AF+  W +AC++++     +IP G F  G + F+GPC ++ 
Sbjct: 34  LVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVS 93

Query: 148 ITIEIKGTVKATTDISEY-SSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKKNINC 207
               +   VKA+TD+S+Y S   W     I GL LTG G FDGQGA AW +N+C  + NC
Sbjct: 94  ---NLTVRVKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNC 153

Query: 208 QSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLS 267
           + LP S+KF  +N T+V  I+S+NSK FH ++  C +F  T ++I AP +SPNTDG+H+ 
Sbjct: 154 KLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIE 213

Query: 268 TSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLV 327
            S  V  S S + TGDDCVSIGQG  +IT+T+I CGPGHGISVGSLG+YP EK V G++V
Sbjct: 214 RSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVV 273

Query: 328 QNCTIFNATNGARIKTWAGTVS-GSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKA 387
           ++C I   TNG RIKTWA +    +AT + F++I+M NV  PIIIDQ+Y        N  
Sbjct: 274 KDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVP 333

Query: 388 SKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINL----TYGG----NNLRN 447
           SK ++S+++FKNIRGTS + VAV L CS   PC+ V L +++L    + GG    +N  N
Sbjct: 334 SKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQSSNRGN 393

Query: 448 TTIVSSCLNAKIKHHGMQNPSPC 457
             + SSC N +  + G Q P PC
Sbjct: 394 EAVSSSCRNVRANYIGTQIPPPC 413

[Close]

BLAST of Lcy03g001030 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 339.3 bits (869), Expect = 4.7e-93
Identity = 164/347 (47.26%), Postives = 228/347 (65.71%), Query Frame = 0


Query: 82  NRKGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAG 141
           N     ++D+ K+GA  DG T+  +AF+ TWI+ C ++  PA  L+P GTFLAGPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVC-DSPVPATLLVPKGTFLAGPVIFAG 104

Query: 142 PCQSLPITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCK 201
           PC+S  +T+ + GT+ ATT  S Y++PEWF  E +  L+LTG+G F G+G + W  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 202 KNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTD 261
           K + C   P S+KF  + +  ++GI+S+N+KAFH  +    N    N+ + AP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 262 GMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSV 321
           G+HLS +  V+I +S + TGDDCVS+G+G   +TV  + CGPGHG+SVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 322 FGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKA 381
            G+ V NCT+    NG RIKTW G+    A  I F++I+M +VK PIIIDQ YG++    
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD- 344

Query: 382 SKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGG 429
           S+  ISD+ FKNIRGT++T   V + CS   PC+GV + D+NL Y G
Sbjct: 345 SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVG 386

[Close]

BLAST of Lcy03g001030 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-89
Identity = 183/412 (44.42%), Postives = 238/412 (57.77%), Query Frame = 0


Query: 52  AAAPQALGIGTLTGRGGSVTVNDVKAVVDRNRKGGAIFDVRKYGAKADGKTDDAQAFMTT 111
           A A +       T  GG+     V A V   + GGA  DV+  GAK DGKTDD+ AF   
Sbjct: 36  ATAVEDTAKNAATAVGGAAA--SVGAKVTGAKPGGASLDVKASGAKGDGKTDDSAAFAAA 95

Query: 112 WIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLPITIEIKGTVKATTDISEYSSPE-- 171
           W EAC   A  +   +P G +L   + F GPC+  P+T+E+ G  KA   + + + P   
Sbjct: 96  WKEAC---AAGSTITVPKGEYLVESLEFKGPCKG-PVTLELNGNFKAPATV-KTTKPHAG 155

Query: 172 WFSIEGITGLILTGS-GVFDGQGASAWSYNDCKKNINCQSLPISIKFSKLNHTIVDGITS 231
           W   E +    L G+  +FDGQG+ AW  NDC K   C SLPI+I+F+ L ++ ++ ITS
Sbjct: 156 WIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITS 215

Query: 232 LNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLSTSKLVTISNSVMGTGDDCVSIG 291
            NSK FH ++ NC N T T++ I AP  S NTDG+H+  S  V +  + + TGDDCVSIG
Sbjct: 216 TNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIG 275

Query: 292 QGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWAGTVS 351
            G E + V N+ CGPGHGIS+GSLG+YP E+ V GV V+ C I N  NG RIKTW G+  
Sbjct: 276 DGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPP 335

Query: 352 GSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKISDVHFKNIRGTSVTNVAV 411
           G A+ I+F+DI M NV  P++IDQ Y      K    SK K+SDV  KNI+GTS T VAV
Sbjct: 336 GIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAV 395

Query: 412 LLECSALFPCEGVELRDINLTYGGNNLRNTTIVSSCLNAKIKHHGMQNPSPC 457
            L CS   PC  + L DINL + G   +    VS+C N K    G   P+ C
Sbjct: 396 KLMCSKGVPCTNIALSDINLVHNG---KEGPAVSACSNIKPILSGKLVPAAC 437

[Close]

BLAST of Lcy03g001030 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-86
Identity = 171/380 (45.00%), Postives = 225/380 (59.21%), Query Frame = 0


Query: 84  KGGAIFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPC 143
           K GA  DV+  GAK D KTDD+ AF   W EAC   A  +   +P G ++   + F GPC
Sbjct: 65  KPGAAVDVKASGAKGDSKTDDSAAFAAAWKEAC---AAGSTITVPKGEYMVESLEFKGPC 124

Query: 144 QSLPITIEIKGTVKATTDISEYSSPE--WFSIEGITGLILTGS-GVFDGQGASAWSYNDC 203
           +  P+T+E+ G  KA   + + + P   W   E I    L G+  +FDGQG+ AW  NDC
Sbjct: 125 KG-PVTLELNGNFKAPATV-KTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDC 184

Query: 204 KKNINCQSLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNT 263
            K   C SLPI+I+F+ L ++ ++ ITS NSK FH ++ NC N T +++ I AP  S NT
Sbjct: 185 AKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNT 244

Query: 264 DGMHLSTSKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKS 323
           DG+H+  S  V +  + + TGDDCVSIG G E + V N+ CGPGHGIS+GSLG+YP E+ 
Sbjct: 245 DGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQP 304

Query: 324 VFGVLVQNCTIFNATNGARIKTWAGTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GT 383
           V GV V+ C I N  NG RIKTW G+  G A+ I+F+DI M NV  P++IDQ Y      
Sbjct: 305 VKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHC 364

Query: 384 KKNKASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTI 443
           K    S+ K+SDV  K I+GTS T VAV L CS   PC  + L DINL + G   +    
Sbjct: 365 KAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNG---KEGPA 424

Query: 444 VSSCLNAKIKHHGMQNPSPC 457
           VS+C N K    G   P+ C
Sbjct: 425 VSACSNIKPILSGKLVPAAC 436

[Close]

BLAST of Lcy03g001030 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 299.3 bits (765), Expect = 5.3e-81
Identity = 159/376 (42.29%), Postives = 231/376 (61.44%), Query Frame = 0


Query: 88  IFDVRKYGAKADGKTDDAQAFMTTWIEACRNTAGPAKFLIPPGTFLAGPVTFAGPCQSLP 147
           IFDVR YGA+AD + D+A AF   W EAC+ + G +   IP G F    VTF+GPC+S  
Sbjct: 31  IFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSGIFYLRQVTFSGPCKS-S 90

Query: 148 ITIEIKGTVKATTDISEYSSPEWFSIEGITGLILTGSGVFDGQGASAWSYNDCKKNINCQ 207
           IT  I+GT+ A  +    +  EW   + +  L +TG G+ DGQG+ +W  NDC KN NC+
Sbjct: 91  ITFFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGGGLLDGQGSYSWPLNDCNKNTNCR 150

Query: 208 SLPISIKFSKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMHIIAPGNSPNTDGMHLST 267
           +L ++I F+ +  + ++G+ S+NSK  H ++F+  +F  T + I APG+SPNTDG+ +  
Sbjct: 151 TLAMNIGFAFVKSSKINGLRSINSKMGHFNLFSVEDFNITGVTITAPGDSPNTDGIKIGK 210

Query: 268 SKLVTISNSVMGTGDDCVSIGQGCEKITVTNITCGPGHGISVGSLGKYPKEKSVFGVLVQ 327
           S  + I N  +GTGDDC++I  G   + ++++ CGPGHGISVGSLG+Y +EK+V G+ V+
Sbjct: 211 SSHMQIYNVTIGTGDDCIAILDGTSNLDISDVRCGPGHGISVGSLGRYKEEKNVQGLTVR 270

Query: 328 NCTIFNATNGARIKTWAGTVSG-SATGIIFDDIVMYNVKYPIIIDQTY------GTKKNK 387
           N  I   T+G RIKTWA +VS  S +  ++++I M NV  PI+IDQ Y       +    
Sbjct: 271 NSIINGTTDGLRIKTWAKSVSQISVSNFLYENIQMINVGNPIVIDQQYCPHGQCDSPGKY 330

Query: 388 ASKWKISDVHFKNIRGTSVTNVAVLLECSALFPCEGVELRDINLTYGGNNLRNTTIVSSC 447
           AS  +I DV + NI GTS +  A+ ++CS  FPC+ VEL +INL Y G   R+  + + C
Sbjct: 331 ASHVQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINLHYVG---RDGLVTALC 390

Query: 448 LNAKIKHHGMQNPSPC 457
            N      G   P+ C
Sbjct: 391 ENVGGSIRGKIVPANC 402

The following BLAST results are available for this feature:

BLAST of Lcy03g001030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	6.6e-92	47.26	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q39786	1.2e-88	45.41	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
Q6H9K0	2.6e-88	43.62	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q39766	4.4e-88	45.14	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
P35339	2.4e-86	44.10	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]

BLAST of Lcy03g001030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JX85	6.0e-205	78.00	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1	[more]
A0A6J1GQZ3	2.1e-202	77.66	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111456314 PE=3 SV=...	[more]
A0A5A7U4W1	1.1e-198	77.65	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7TQ89	7.1e-198	77.43	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BRD3	1.9e-190	73.26	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1	[more]

BLAST of Lcy03g001030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896336.1	2.4e-208	79.35	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_022991773.1	1.2e-204	78.00	exopolygalacturonase-like [Cucurbita maxima]	[more]
KAG7014351.1	3.6e-204	78.31	Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_023549400.1	4.7e-204	78.52	exopolygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6575809.1	2.3e-203	77.87	Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lcy03g001030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18180.1	3.6e-93	46.21	Pectin lyase-like superfamily protein	[more]
AT1G02790.1	4.7e-93	47.26	polygalacturonase 4	[more]
AT3G14040.1	1.8e-89	44.42	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	1.5e-86	45.00	Pectin lyase-like superfamily protein	[more]
AT2G33160.1	5.3e-81	42.29	glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 268..289 e-value: 700.0 score: 8.3 coord: 291..311 e-value: 3100.0 score: 3.7 coord: 351..372 e-value: 4100.0 score: 2.9 coord: 241..267 e-value: 3800.0 score: 3.1 coord: 321..342 e-value: 16.0 score: 14.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 74..456 e-value: 5.3E-108 score: 363.6
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 121..433 e-value: 1.2E-90 score: 303.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 298..311
None	No IPR available	PANTHER	PTHR31375:SF158	POLYGALACTURONASE	coord: 2..453
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..453
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..457

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g001030.1	Lcy03g001030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity