Lcy03g000730 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g000730
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	nicotianamine synthase-like
Location	Chr03: 2067306 .. 2069028 (+)
RNA-Seq Expression	Lcy03g000730
Synteny	Lcy03g000730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAGACCTTTCCATTTCTCTCAACCCTCTCTCTCTCTCTCTCCTTTTCTTTTTGCTCATAGTGTCGACATGGTTTGTGAAGAAGAAGCTCTAATCCAAAAGGTGTCCGATTTGTATGAGCAAATCTCTTGCCTTGAAAGCCTCAAACCTTGTAAGCATGTTGACACTCTCTTCACTGAGCTGGTGGGCACGTGCATGGCACCCACGAGCCCTCATTTCCACATCAATTCACTCTCAAACCCTCTTCAGCAAATGAGATCAAATCTCATTCGCCTTTGTGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAAATTTGATTCCCCTCTTCACCATCTCCATGTCTTCCCTTACTTCTCTAACTACCTCAAACTTAGCCTCCTTGAATACTCCATCCTCCGCCGCCACTGCCCCACCGTCCCCTCCACCGTCGCCTTCGTCGGCTCCGGCCCTCTTCCTTTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCGCCACTACCTTCCACAATTACGATCTCGACCCGCTTGCCAATTCCCAGGTGATTGTCATCTGCATTCTCGATTCGTGTATCGGACTTAATTGCAAATTTTCATTGTGCTTTTTTCCTTTTCGATAAAATAGTAAATTTATCCTAACCCATTGGCTTGAATGCTTTTTGAGAAATGTTATTTTCTCTTCGATTAATATCGATATCCACTTATTGGATCTTGTGTGAATTTCTAAGTTTAACTTTTGAATTGATGGGTGACTTAATTAACGGTAAATATGGGCAAAATTAAACGAAACTAATAAACTCATGTTTTCTGTTGATGTACAGGCGTCGAATTTGGTATCGTCGGACCCGGACTTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACCGAGGAGCTGAAACAGTACGAGGTAGTGTTTTTGGCAGCTCTTGTGGGAATGGAGAAAGAGGAGAAATTGAGAGTGATTGAGCATTTGTCTGAGTTCATGTCGGAAGGGGCTTATTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTTCTTTATCCTGTGGTTGAGGATTGTGATTTGGTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACCGACGAAGTCATAAACTCCGTAATACTCGCACGAAAAAAGACGAGATTCGAGGCCGAGAACGAGAACGAGGATGACGGTTGTTGTTTGTCTGTTAATTCAGACAAAAAAGAGGAAGATGATAATGAGAAGGTAATGATTAGTAGCGGTATTGAAAATGGTGTTGTGCATAATAATAATAAATGTTCTGAGATTCAAAGCTTCAATAATCATGGAGGTATGATAGAGGAATTGGCCATGGAAGCTGAGTAATTCACTTGAATTGTTATGGTTAAGTGGGTTTCTGATTCTGATTCTGATTCTAGTTTTGGTTTTGGTGCTACAAAAATGTGTCTTTTCAACATGGGGACTTTGATTTTCTTGAGGAACATCCATGGGGGAAGAGCAAGTTTATAAGGTTTTATATTTTGTTTTCACTTTTTTTTTTTTTAGTTTGTTTATGTTTTTAGGGTTAATTGCAAATGGACCCTTCAGTTATGAAGGTTGATATGTTTTGTAATAATTTTTGTGTTCTCACTTTTGGATGAGAATCTTGTTTGGCTACTGCTTTTTCTGTTTCTGAATCTTGAATTTTCAATAGGGTAATGAGAG

mRNA sequence

AAAAAAAAGACCTTTCCATTTCTCTCAACCCTCTCTCTCTCTCTCTCCTTTTCTTTTTGCTCATAGTGTCGACATGGTTTGTGAAGAAGAAGCTCTAATCCAAAAGGTGTCCGATTTGTATGAGCAAATCTCTTGCCTTGAAAGCCTCAAACCTTGTAAGCATGTTGACACTCTCTTCACTGAGCTGGTGGGCACGTGCATGGCACCCACGAGCCCTCATTTCCACATCAATTCACTCTCAAACCCTCTTCAGCAAATGAGATCAAATCTCATTCGCCTTTGTGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAAATTTGATTCCCCTCTTCACCATCTCCATGTCTTCCCTTACTTCTCTAACTACCTCAAACTTAGCCTCCTTGAATACTCCATCCTCCGCCGCCACTGCCCCACCGTCCCCTCCACCGTCGCCTTCGTCGGCTCCGGCCCTCTTCCTTTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCGCCACTACCTTCCACAATTACGATCTCGACCCGCTTGCCAATTCCCAGGCGTCGAATTTGGTATCGTCGGACCCGGACTTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACCGAGGAGCTGAAACAGTACGAGGTAGTGTTTTTGGCAGCTCTTGTGGGAATGGAGAAAGAGGAGAAATTGAGAGTGATTGAGCATTTGTCTGAGTTCATGTCGGAAGGGGCTTATTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTTCTTTATCCTGTGGTTGAGGATTGTGATTTGGTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACCGACGAAGTCATAAACTCCGTAATACTCGCACGAAAAAAGACGAGATTCGAGGCCGAGAACGAGAACGAGGATGACGGTTGTTGTTTGTCTGTTAATTCAGACAAAAAAGAGGAAGATGATAATGAGAAGGTAATGATTAGTAGCGGTATTGAAAATGGTGTTGTGCATAATAATAATAAATGTTCTGAGATTCAAAGCTTCAATAATCATGGAGGTATGATAGAGGAATTGGCCATGGAAGCTGAGTAATTCACTTGAATTGTTATGGTTAAGTGGGTTTCTGATTCTGATTCTGATTCTAGTTTTGGTTTTGGTGCTACAAAAATGTGTCTTTTCAACATGGGGACTTTGATTTTCTTGAGGAACATCCATGGGGGAAGAGCAAGTTTATAAGGTTTTATATTTTGTTTTCACTTTTTTTTTTTTTAGTTTGTTTATGTTTTTAGGGTTAATTGCAAATGGACCCTTCAGTTATGAAGGTTGATATGTTTTGTAATAATTTTTGTGTTCTCACTTTTGGATGAGAATCTTGTTTGGCTACTGCTTTTTCTGTTTCTGAATCTTGAATTTTCAATAGGGTAATGAGAG

Coding sequence (CDS)

ATGGTTTGTGAAGAAGAAGCTCTAATCCAAAAGGTGTCCGATTTGTATGAGCAAATCTCTTGCCTTGAAAGCCTCAAACCTTGTAAGCATGTTGACACTCTCTTCACTGAGCTGGTGGGCACGTGCATGGCACCCACGAGCCCTCATTTCCACATCAATTCACTCTCAAACCCTCTTCAGCAAATGAGATCAAATCTCATTCGCCTTTGTGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAAATTTGATTCCCCTCTTCACCATCTCCATGTCTTCCCTTACTTCTCTAACTACCTCAAACTTAGCCTCCTTGAATACTCCATCCTCCGCCGCCACTGCCCCACCGTCCCCTCCACCGTCGCCTTCGTCGGCTCCGGCCCTCTTCCTTTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCGCCACTACCTTCCACAATTACGATCTCGACCCGCTTGCCAATTCCCAGGCGTCGAATTTGGTATCGTCGGACCCGGACTTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACCGAGGAGCTGAAACAGTACGAGGTAGTGTTTTTGGCAGCTCTTGTGGGAATGGAGAAAGAGGAGAAATTGAGAGTGATTGAGCATTTGTCTGAGTTCATGTCGGAAGGGGCTTATTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTTCTTTATCCTGTGGTTGAGGATTGTGATTTGGTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACCGACGAAGTCATAAACTCCGTAATACTCGCACGAAAAAAGACGAGATTCGAGGCCGAGAACGAGAACGAGGATGACGGTTGTTGTTTGTCTGTTAATTCAGACAAAAAAGAGGAAGATGATAATGAGAAGGTAATGATTAGTAGCGGTATTGAAAATGGTGTTGTGCATAATAATAATAAATGTTCTGAGATTCAAAGCTTCAATAATCATGGAGGTATGATAGAGGAATTGGCCATGGAAGCTGAGTAA

Protein sequence

MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQQMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCPTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEEDDNEKVMISSGIENGVVHNNNKCSEIQSFNNHGGMIEELAMEAE

Homology

[Close]

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.1e-94
Identity = 169/273 (61.90%), Postives = 220/273 (80.59%), Query Frame = 0


Query: 3   CEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQQM 62
           C+++ L+ K+ DLYE+IS LE+LKPC+ VDTLF +LV TC+ P +P+  +  +S  +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRHC-P 122
           RSNLI++CG+AE  LE HFS +L  F D+PLHHL++FPY++NYLKLS LE+ +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 182
            VP TVAF+GSGPLP TS+VLA+ HL  + FHN+D+DP AN  A+ LVSSDPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K+EK++V+EHL + MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARK 274
           YP+VE CDL GFEVLSV+HPTDEVINS++++RK
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.0e-93
Identity = 184/344 (53.49%), Postives = 243/344 (70.64%), Query Frame = 0


Query: 1   MVC-EEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPL 60
           MVC     +++KV +LYEQIS LE+L P K V+ LFT+LV TCM P      ++ L   +
Sbjct: 1   MVCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP--IDVSKLCQKI 60

Query: 61  QQMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHC 120
           Q++RS+LI+LCGQAE LLESHFS +L+ +++PL HLH+FPYF NY+KLSLLEY+IL ++ 
Sbjct: 61  QEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNT 120

Query: 121 PTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMV 180
             +P  +AF+GSGPLP TS+VLAT+HL  T FHNYD+D  AN  AS LV++DPD+ +RM 
Sbjct: 121 TNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMT 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAF 240
           FHT D+M VT  LK Y+VVFLAALVGM+KE+K++V++HL+++MS GA LM+RSAHG RAF
Sbjct: 181 FHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEE 300
           LYPV++  DL GFEVLSV+HPTDEVINSVI+ARK                L V S     
Sbjct: 241 LYPVLDPRDLRGFEVLSVYHPTDEVINSVIIARK----------------LPVPS----- 300

Query: 301 DDNEKVMISSGIENGVVHNNNKCSEIQSFN--NHGGMIEELAME 342
                V +  G+   V+ +   C+EI +FN  N   ++EE A+E
Sbjct: 301 -----VPLLDGLGAYVLPSKCACAEIHAFNPLNKMNLVEEFALE 316

[Close]

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.8e-90
Identity = 173/310 (55.81%), Postives = 228/310 (73.55%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           M C++E L+Q + DLYE+IS LESLKP + V+ LF +LV TC+ P +P+  +  + + +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRHC 120
           ++R NLI++CG AE  LE+HFS +L  + D+PLHHL++FPY++NYLKL  LE+ +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 -PTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRM 180
              VP +VAF+GSGPLP TSIVLA+ HL  T FHN+D+DP ANS AS LVSSDPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK++VIEHL + M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLS-VNSDKK 300
           FLYP+VE CDL GFEVLS++HPTD+VINSV++++K       N    + C L   N  K 
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVVSIGNVGGPNSCLLKPCNCSKT 300

Query: 301 EEDDNEKVMI 308
               N+ +MI
Sbjct: 301 HAKMNKNMMI 309

[Close]

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.3e-88
Identity = 159/275 (57.82%), Postives = 211/275 (76.73%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSL-SNPL 60
           M CE   +++++ DLY QIS LESLKP K+VDTLF +LV TC+ PT  +  +  +    +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRH 120
           + MRS+LI+LCG+AE  LE HFS +L  F D+PL+HLH+FPY++NYLKL  LE+ +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRM 180
              VP+ VAF+GSGP+P TSIVLA  HL  TTFHN+D+D  AN+ ASNLVS D DL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRA 240
           +FHT D++   E L QY+VVFLAALVGM+KE K++ IEHL + M+ GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARK 274
           FLYP+V+ CDL GFEVL+++HP+D+V+NSV++ARK
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.2e-87
Identity = 159/291 (54.64%), Postives = 213/291 (73.20%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           M C+   +++++ DLY+QIS L+SLKP K+VDTLF +LV TC+ PT  +  + ++   ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKF---DSPLHHLHVFPYFSNYLKLSLLEYSILRR 120
            MR+NLI+LCG+AE  LE HFS +L       +PL HLH+FPY+SNYLKL  LE+ +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTR 180
           H   VP+ +AFVGSGP+P TSIVLA  HL  TTFHN+D+D  AN+ ASNLVS DPDL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGR 240
           M+FHT D++  TE L QY+VVFLAALVGM+KE K++ IEHL + M+ GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGC 289
           AFLYP+V+  DL GF++L+++HPTD+V+NSV++ARK         N   GC
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARKLGGPTTPGVNGTRGC 290

[Close]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL
Match: A0A1S3BPH1 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 285/353 (80.74%), Postives = 312/353 (88.39%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MVCEEE LIQ+V +LY++IS LESLKPC +VDTLFT+LV TCM+PTSPHFHINSLS PLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRS+LI LCGQAESLLESHFSDLLAKFDSP+ HLHVFPYFSNYLKLSLLE+SILRRH P
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 TV-PSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMV 180
            V PS VAFVGSGPLP TSIVLATRHLT+T FHNYDLDP+ANS+ASNLVS DPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKL+VI+HLS++MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAE---NENEDDGCCLSVNSDK 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVI+ARK T+++ E   N+N DD CCL VNS  
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNS-- 300

Query: 301 KEEDDNEKVMISSGIE-----NGVVHNNNKCSEIQS--FNNHGGMIEELAMEA 343
              D+NEKV ISSGI+     NGVVH NNKCSEIQ+   NNHGG IEE AMEA
Sbjct: 301 ---DENEKVKISSGIDHKKDNNGVVH-NNKCSEIQNGFNNNHGGKIEEFAMEA 347

[Close]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL
Match: A0A0A0LVX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.9e-148
Identity = 281/349 (80.52%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MVCEEE LIQKV +LY++IS LESLKPC +VDTLFT+LV TCM+PTSPHFHINSLS PLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLE HFSDLLAKFDSP+ HLHVFPYFSNY+KLSLLE+SIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMV 180
             +PS VAFVGSGPLP TS+VLATRHLT+T FHNYDLDP+ANS+ASNLVS DPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKL+VI+HLSE+MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENE-----NEDDGCCLSVNS 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVI+ARK  +F+ +N+     N D+GCCL VNS
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DKKEEDDNEKVMISSGIENGVVHNNNKCSEIQS-FNNHGGMIEELAMEA 343
                D+NE V ISS    GVVH NNKCSEIQ+ FNNHGG IEE AMEA
Sbjct: 301 -----DENENVKISS----GVVH-NNKCSEIQNGFNNHGGKIEEFAMEA 339

[Close]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL
Match: A0A6J1JPC1 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.2e-145
Identity = 276/340 (81.18%), Postives = 290/340 (85.29%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MV EEEALIQKV DLYEQIS LESLKPCKHVDTLFTELV TCM PTSP+FHINSLSNPLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLESHFSDLLA FD PLHHLH+FPYFSNYLKLSLLE+SILRRHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 180
            +PS+VAFVGSGPLP TSIVLATRHL +T+FHNYDLDPLANSQAS LVS D DLKTRMVF
Sbjct: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKLRVIEHL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEED 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVI+ARKK         +DD C   V  D  E  
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK--------KKDDDCLKIVGDDNHE-- 300

Query: 301 DNEKVMISSGIENG-VVHNNNKCSEIQSFNNHGGMIEELA 340
                   +GIENG VVHN   C  I  FNNHGGMIEELA
Sbjct: 301 --------NGIENGVVVHNKQCCDHIHGFNNHGGMIEELA 322

[Close]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL
Match: A0A6J1EU11 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.7e-143
Identity = 274/340 (80.59%), Postives = 291/340 (85.59%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MV EEEALIQKV DLYEQIS LESLKPCKHVDTLFTELV TCM PTSP+FHINSLSNPLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLESHFSDLLA FD PLHHLH+FPYFSNYLKLSLLE+SILRRHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 180
            +PS+VAFVGSGPLP TSIVLATRHL +T+FHNYDLDPLAN+QAS LVS D DLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANTQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKLRVIEHL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEED 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVI+ARKK     + +++DD C   V       D
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK-----KKDHDDDDCLKIVG------D 300

Query: 301 DNEKVMISSGIENG-VVHNNNKCSEIQSFNNHGGMIEELA 340
           DN         ENG VVHN   C  I  FNNHGGMIEELA
Sbjct: 301 DNH--------ENGVVVHNKQCCDHIHGFNNHGGMIEELA 321

[Close]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL
Match: A0A5A7U6Y1 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004490 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.4e-120
Identity = 237/292 (81.16%), Postives = 257/292 (88.01%), Query Frame = 0


Query: 62  MRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCPT 121
           MRS+LI LCGQAESLLESHFSDLLAKFDSP+ HLHVFPYFSNYLKLSLLE+SILRRH P 
Sbjct: 1   MRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 60

Query: 122 V-PSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 181
           V PS VAFVGSGPLP TSIVLATRHLT+T FHNYDLDP+ANS+ASNLVS DPDLKTRMVF
Sbjct: 61  VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 120

Query: 182 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 241
           HTCDIMKVTEELKQYEVVFLAALVGMEK+EKL+VI+HLS++MSEGAYLMVRSAHGGRAFL
Sbjct: 121 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 180

Query: 242 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAE---NENEDDGCCLSVNSDKK 301
           YPVVED DL+GFEVLSVFHPTDEVINSVI+ARK T+++ E   N+N DD CCL VNS   
Sbjct: 181 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNS--- 240

Query: 302 EEDDNEKVMISSGIE-----NGVVHNNNKCSEIQS--FNNHGGMIEELAMEA 343
             D+NEKV ISSGI+     NGVVH NNKCSEIQ+   NNHGG IEE AMEA
Sbjct: 241 --DENEKVKISSGIDHKKDNNGVVH-NNKCSEIQNGFNNNHGGKIEEFAMEA 286

[Close]

BLAST of Lcy03g000730 vs. NCBI nr
Match: XP_008450545.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo])

HSP 1 Score: 539.3 bits (1388), Expect = 2.5e-149
Identity = 285/353 (80.74%), Postives = 312/353 (88.39%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MVCEEE LIQ+V +LY++IS LESLKPC +VDTLFT+LV TCM+PTSPHFHINSLS PLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRS+LI LCGQAESLLESHFSDLLAKFDSP+ HLHVFPYFSNYLKLSLLE+SILRRH P
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 TV-PSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMV 180
            V PS VAFVGSGPLP TSIVLATRHLT+T FHNYDLDP+ANS+ASNLVS DPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKL+VI+HLS++MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAE---NENEDDGCCLSVNSDK 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVI+ARK T+++ E   N+N DD CCL VNS  
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNS-- 300

Query: 301 KEEDDNEKVMISSGIE-----NGVVHNNNKCSEIQS--FNNHGGMIEELAMEA 343
              D+NEKV ISSGI+     NGVVH NNKCSEIQ+   NNHGG IEE AMEA
Sbjct: 301 ---DENEKVKISSGIDHKKDNNGVVH-NNKCSEIQNGFNNNHGGKIEEFAMEA 347

[Close]

BLAST of Lcy03g000730 vs. NCBI nr
Match: XP_038878580.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 536.2 bits (1380), Expect = 2.1e-148
Identity = 275/321 (85.67%), Postives = 295/321 (91.90%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MVCEEEALIQKVS+LY++IS LESLKPC HVDTLFT+LV TCM+PTSPHFHINSLS  LQ
Sbjct: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKSLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLE HFSDLLAKFDSP+HHLHVFPYFSNYLKLSLLE+SILRRHCP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 180
            +PS VAFVGSGPLP TSIVLATRHLT+T FHNYDLD LANS+ASNLVS DPDLKTRMVF
Sbjct: 121 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDLLANSKASNLVSLDPDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVI+HLSEFMSEGAYLMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIKHLSEFMSEGAYLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEED 300
           YPVVEDCDLVGFEVLSVFHPTDEVINSVI+A+KK  F+  N+N+D+   LSVNSDK EED
Sbjct: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIAQKKMIFDDNNDNDDNRDYLSVNSDKLEED 300

Query: 301 DNEKVMIS--SGIENGVVHNN 320
           +NEKVMIS      NGV+HN+
Sbjct: 301 ENEKVMISGFDRKNNGVMHND 321

[Close]

BLAST of Lcy03g000730 vs. NCBI nr
Match: XP_004135580.1 (nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_007577 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 6.1e-148
Identity = 281/349 (80.52%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MVCEEE LIQKV +LY++IS LESLKPC +VDTLFT+LV TCM+PTSPHFHINSLS PLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLE HFSDLLAKFDSP+ HLHVFPYFSNY+KLSLLE+SIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMV 180
             +PS VAFVGSGPLP TS+VLATRHLT+T FHNYDLDP+ANS+ASNLVS DPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKL+VI+HLSE+MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENE-----NEDDGCCLSVNS 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVI+ARK  +F+ +N+     N D+GCCL VNS
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DKKEEDDNEKVMISSGIENGVVHNNNKCSEIQS-FNNHGGMIEELAMEA 343
                D+NE V ISS    GVVH NNKCSEIQ+ FNNHGG IEE AMEA
Sbjct: 301 -----DENENVKISS----GVVH-NNKCSEIQNGFNNHGGKIEEFAMEA 339

[Close]

BLAST of Lcy03g000730 vs. NCBI nr
Match: KAG6588596.1 (putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 526.2 bits (1354), Expect = 2.2e-145
Identity = 275/340 (80.88%), Postives = 294/340 (86.47%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MV EEEALIQKV DLYEQIS LESLKPCKHVDTLFTELV TCMAPTSP+FHINSLSNPLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMAPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLESHFSDLLA FD PLHHLH+FPYFSNYLKLSLLE+SILRRHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 180
            +PS+VAFVGSGPLP TSIVLATRHL +T+FHNYD+DPLAN+QAS LVS D DLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDIDPLANTQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKLRVIEHL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEED 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVI+ARKK     + +++DD C   V  D  E  
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK-----KKDHDDDDCLKIVGDDNHE-- 300

Query: 301 DNEKVMISSGIENG-VVHNNNKCSEIQSFNNHGGMIEELA 340
                   +GIENG VVHN   C  I  FNNHGGMIEELA
Sbjct: 301 --------NGIENGVVVHNKQCCDHIHGFNNHGGMIEELA 325

[Close]

BLAST of Lcy03g000730 vs. NCBI nr
Match: XP_023529837.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.8 bits (1353), Expect = 2.8e-145
Identity = 275/340 (80.88%), Postives = 294/340 (86.47%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           MV EEEALIQKV DLYEQIS LESLKPCKHVDTLFTELV TCM PTSP+FHINSLSNP+Q
Sbjct: 1   MVTEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKFDSPLHHLHVFPYFSNYLKLSLLEYSILRRHCP 120
           QMRSNLIRLCGQAESLLESHFSDLLA FD PLHHLH+FPYFSNYLKLSLLE+SILRRHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 180
            +PS+VAFVGSGPLP TSIVLATRHL +T+FHNYDLDPLANSQAS LVS D DLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDADLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKLRVIEHL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLSVNSDKKEED 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVI+ARKK     + +++DD  CL +  D   E 
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK-----KKDHDDDDDCLKIVGDDNHE- 300

Query: 301 DNEKVMISSGIENG-VVHNNNKCSEIQSFNNHGGMIEELA 340
                   +GIENG VVHN   C  I  FNNHGGMIEELA
Sbjct: 301 --------NGIENGVVVHNKQCCDHIHGFNNHGGMIEELA 326

[Close]

BLAST of Lcy03g000730 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 346.7 bits (888), Expect = 2.2e-95
Identity = 169/273 (61.90%), Postives = 220/273 (80.59%), Query Frame = 0


Query: 3   CEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQQM 62
           C+++ L+ K+ DLYE+IS LE+LKPC+ VDTLF +LV TC+ P +P+  +  +S  +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRHC-P 122
           RSNLI++CG+AE  LE HFS +L  F D+PLHHL++FPY++NYLKLS LE+ +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 TVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRMVF 182
            VP TVAF+GSGPLP TS+VLA+ HL  + FHN+D+DP AN  A+ LVSSDPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K+EK++V+EHL + MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDCDLVGFEVLSVFHPTDEVINSVILARK 274
           YP+VE CDL GFEVLSV+HPTDEVINS++++RK
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lcy03g000730 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 332.0 bits (850), Expect = 5.6e-91
Identity = 173/310 (55.81%), Postives = 228/310 (73.55%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           M C++E L+Q + DLYE+IS LESLKP + V+ LF +LV TC+ P +P+  +  + + +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRHC 120
           ++R NLI++CG AE  LE+HFS +L  + D+PLHHL++FPY++NYLKL  LE+ +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 -PTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRM 180
              VP +VAF+GSGPLP TSIVLA+ HL  T FHN+D+DP ANS AS LVSSDPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK++VIEHL + M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGCCLS-VNSDKK 300
           FLYP+VE CDL GFEVLS++HPTD+VINSV++++K       N    + C L   N  K 
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVVSIGNVGGPNSCLLKPCNCSKT 300

Query: 301 EEDDNEKVMI 308
               N+ +MI
Sbjct: 301 HAKMNKNMMI 309

[Close]

BLAST of Lcy03g000730 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 326.2 bits (835), Expect = 3.1e-89
Identity = 159/275 (57.82%), Postives = 211/275 (76.73%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSL-SNPL 60
           M CE   +++++ DLY QIS LESLKP K+VDTLF +LV TC+ PT  +  +  +    +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLESHFSDLLAKF-DSPLHHLHVFPYFSNYLKLSLLEYSILRRH 120
           + MRS+LI+LCG+AE  LE HFS +L  F D+PL+HLH+FPY++NYLKL  LE+ +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTRM 180
              VP+ VAF+GSGP+P TSIVLA  HL  TTFHN+D+D  AN+ ASNLVS D DL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGRA 240
           +FHT D++   E L QY+VVFLAALVGM+KE K++ IEHL + M+ GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARK 274
           FLYP+V+ CDL GFEVL+++HP+D+V+NSV++ARK
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lcy03g000730 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 322.4 bits (825), Expect = 4.4e-88
Identity = 159/291 (54.64%), Postives = 213/291 (73.20%), Query Frame = 0


Query: 1   MVCEEEALIQKVSDLYEQISCLESLKPCKHVDTLFTELVGTCMAPTSPHFHINSLSNPLQ 60
           M C+   +++++ DLY+QIS L+SLKP K+VDTLF +LV TC+ PT  +  + ++   ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLAKF---DSPLHHLHVFPYFSNYLKLSLLEYSILRR 120
            MR+NLI+LCG+AE  LE HFS +L       +PL HLH+FPY+SNYLKL  LE+ +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPTVPSTVAFVGSGPLPFTSIVLATRHLTATTFHNYDLDPLANSQASNLVSSDPDLKTR 180
           H   VP+ +AFVGSGP+P TSIVLA  HL  TTFHN+D+D  AN+ ASNLVS DPDL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIEHLSEFMSEGAYLMVRSAHGGR 240
           M+FHT D++  TE L QY+VVFLAALVGM+KE K++ IEHL + M+ GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEDCDLVGFEVLSVFHPTDEVINSVILARKKTRFEAENENEDDGC 289
           AFLYP+V+  DL GF++L+++HPTD+V+NSV++ARK         N   GC
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARKLGGPTTPGVNGTRGC 290

[Close]

BLAST of Lcy03g000730 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-10
Identity = 32/56 (57.14%), Postives = 41/56 (73.21%), Query Frame = 0


Query: 222 MSEGAYLMVRSAHGGRAFLYPVVEDCDLVGFEVLSVFHP--TDEVINSVILARKKT 276
           M  GA LM+RSA G R+FLY  V+ CDL GFEVL ++HP  +D  +NSV++ARK T
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLT 56

The following BLAST results are available for this feature:

BLAST of Lcy03g000730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	3.1e-94	61.90	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	2.0e-93	53.49	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	7.8e-90	55.81	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FKT9	4.3e-88	57.82	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	6.2e-87	54.64	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]

BLAST of Lcy03g000730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BPH1	1.2e-149	80.74	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1	[more]
A0A0A0LVX1	2.9e-148	80.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1	[more]
A0A6J1JPC1	5.2e-145	81.18	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=...	[more]
A0A6J1EU11	3.7e-143	80.59	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 S...	[more]
A0A5A7U6Y1	4.4e-120	81.16	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of Lcy03g000730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008450545.1	2.5e-149	80.74	PREDICTED: nicotianamine synthase-like [Cucumis melo]	[more]
XP_038878580.1	2.1e-148	85.67	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_004135580.1	6.1e-148	80.52	nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_00...	[more]
KAG6588596.1	2.2e-145	80.88	putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023529837.1	2.8e-145	80.88	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy03g000730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	2.2e-95	61.90	nicotianamine synthase 4	[more]
AT1G09240.1	5.6e-91	55.81	nicotianamine synthase 3	[more]
AT5G56080.1	3.1e-89	57.82	nicotianamine synthase 2	[more]
AT5G04950.1	4.4e-88	54.64	nicotianamine synthase 1	[more]
AT4G26483.1	2.8e-10	57.14	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..275 e-value: 3.7E-118 score: 393.9
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..284
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..278 score: 55.328781
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..275 e-value: 5.6E-61 score: 208.0
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g000730.1	Lcy03g000730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity