Lcy03g000520 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g000520 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.4e-43
Identity = 166/576 (28.82%), Postives = 276/576 (47.92%), Query Frame = 0

Query: 34  KDLEKQLK-YLQDIREKVQ--SLVNEQRSNSYEIDNYVLTWLAEVDGELLEMPQQNEGSN 93
           K LE++L+ +L+ + E V    L+ E+RS+          WL++ D E+LE  ++ E   
Sbjct: 35  KSLERKLRIWLRKVEENVPLGELILEKRSS-------CAIWLSDKDVEILEKVKRLEEQG 94

Query: 94  ISCFNCVQWHQLARKTKKREYNKDNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDA 153
                 +  ++ +R+  +R                    L   +   KT   + +++KD 
Sbjct: 95  QDLIKKISVNKSSREIVER-------------------VLGPSFHPQKTALEMLDKLKDC 154

Query: 154 LADPNVNKVGVCGMGGVGKTKLLDEVKKLVLE---NNLFHRVVVVTVGQLKGATDIQDEV 213
           L   NV K+GV GMGGVGKT L+  +   +L+      F  V+ VTV +      +Q ++
Sbjct: 155 LKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVSKDFDLKRVQMDI 214

Query: 214 KGKLNMELNENN---------KEIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEG 273
             +L                 + ++++K N L+++DD+W   DL+ + GIP   E  K+ 
Sbjct: 215 AKRLGKRFTREQMNQLGLTICERLIDLK-NFLLILDDVWHPIDLD-QLGIPLALERSKDS 274

Query: 274 CKIVITSRFQNLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE-SMEVGIEQIAKSVVKKC 333
            K+V+TSR   + +QM  ++  +V  L E+E+W+ F   VGE +    ++ IAK V  +C
Sbjct: 275 -KVVLTSRRLEVCQQMMTNENIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHEC 334

Query: 334 GGLPIALNILAKTLKGR-NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGEA 393
            GLP+A+  + +TL+G+  V VWK  L  LK   S    D  ++++  LK+ YD +    
Sbjct: 335 CGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR--SAPSIDTEEKIFGTLKLSYDFLQDNM 394

Query: 394 KLLFLMCSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLE 453
           K  FL C++FP+++ I V +L MY +  GLL D   ++ED  +  + LV+ LK S LL  
Sbjct: 395 KSCFLFCALFPEDYSIKVSELIMYWVAEGLL-DGQHHYEDMMNEGVTLVERLKDSCLL-- 454

Query: 454 SNNERDDYVRMHDVVRDVAKYIASKIDMHFEPSLMHS-----------LKNLQTLCMLNF 513
            + +  D V+MHDVVRD A +  S     F   +M             + ++Q + ++  
Sbjct: 455 EDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQDKFVSSVQRVSLMAN 514

Query: 514 TCE------CNSIDTI---------------GELKTLKILRIIFCS--TIEELPESMSQL 559
             E         ++T+               G L+    LRI+  S   I  LP+S S L
Sbjct: 515 KLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILDLSGVRIRTLPDSFSNL 574

BLAST of Lcy03g000520 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 6.7e-41
Identity = 162/622 (26.05%), Postives = 303/622 (48.71%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           ME    ++  +++ +      ++     +K N+K L + L+ L +++  +        + 
Sbjct: 1   MECCAPVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHETLLTK 60

Query: 61  SYEIDNYVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKK-----REYNKDN 120
              +   ++ W  E + E++   +      +SC   ++  +++RK  K     +   KD 
Sbjct: 61  DKPLRLKLMRWQREAE-EVISKARLKLEERVSCGMSLR-PRMSRKLVKILDEVKMLEKDG 120

Query: 121 VG-------HRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVG 180
           +           P      P +   +Q + +  L   +I+D L      K+GV GMGGVG
Sbjct: 121 IEFVDMLSVESTPERVEHVPGVSVVHQTMASNML--AKIRDGLTSEKAQKIGVWGMGGVG 180

Query: 181 KTKLLDEVKKLVLENNL---FHRVVVVTVGQLKGATDIQDEVKGKLNM--ELNENNKEIV 240
           KT L+  +   + E      F  V+ V V +     ++Q ++  +L++  ++ E+ +++ 
Sbjct: 181 KTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLA 240

Query: 241 E------MKD-NILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLLKQMNI 300
                  MK+   L+++DD+WK  DL+   GIP R+E  K G K+++TSRF  + + M  
Sbjct: 241 RRIYVGLMKERKFLLILDDVWKPIDLDL-LGIP-RTEENK-GSKVILTSRFLEVCRSMKT 300

Query: 301 DKWFEVNSLNEEESWKFFVKIVGESMEVG-IEQIAKSVVKKCGGLPIALNILAKTLKG-R 360
           D    V+ L EE++W+ F K  G+ +    + +IAK+V ++CGGLP+A+  +   ++G +
Sbjct: 301 DLDVRVDCLLEEDAWELFCKNAGDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKK 360

Query: 361 NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGEAKLLFLMCSVFPDNHEISV 420
           NV++W  VL KL   +   I+ + ++++  LK+ YD ++ +AK  FL+C++FP+++ I V
Sbjct: 361 NVKLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLEDKAKFCFLLCALFPEDYSIEV 420

Query: 421 EDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDVVRDV 480
            ++  Y M  G + +++ + ED+ +  +  V+ LK  Y LLE  + RD  V+MHDVVRD 
Sbjct: 421 TEVVRYWMAEGFM-EELGSQEDSMNEGITTVESLK-DYCLLEDGDRRDT-VKMHDVVRDF 480

Query: 481 AKYI--ASKIDMHFEPSLMHSLKNLQTLCMLNFTCECNSIDTI-GELKTLKILRIIFC-S 540
           A +I  +S+ D H   SL+ S   LQ +           +  +  +L++L  L   FC  
Sbjct: 481 AIWIMSSSQDDSH---SLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVK 540

Query: 541 TIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWESFERWGEEVSYKN 593
           T   L +    L E+ +  +   P L +++     S T+++          +     +  
Sbjct: 541 TSVLLLQGNFLLKEVPIGFLQAFPTLRILN----LSGTRIKSFPSCSLLRLFSLHSLFLR 600

BLAST of Lcy03g000520 vs. ExPASy Swiss-Prot
Match: Q8RXS5 (Probable disease resistance protein At5g63020 OS=Arabidopsis thaliana OX=3702 GN=At5g63020 PE=2 SV=2)

HSP 1 Score: 160.6 bits (405), Expect = 6.9e-38
Identity = 183/691 (26.48%), Postives = 315/691 (45.59%), Query Frame = 0

Query: 3   TLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREK-VQSLVNEQRSNS 62
           +++I  + + K +   + R   Y+   + N+  L++ L+ ++  RE  ++ +++E+R   
Sbjct: 6   SVSISCDQLTKNVCSCLNRNGDYIHGLEENLTALQRALEQIEQRREDLLRKILSEERRGL 65

Query: 63  YEIDNYVLTWLAEVD------GELLEMPQQNEGSNISCFNC----VQWHQLARKTKKREY 122
             + + V  W+++V+       EL+ M          C  C    V  ++  ++  K   
Sbjct: 66  QRL-SVVQGWVSKVEAIVPRVNELVRMRSVQVQRLCLCGFCSKNLVSSYRYGKRVMKMIE 125

Query: 123 NKDNVGHRAPLPDTKCPTLPADYQILKTRTLLA-----EQIKDALADPNVNKVGVCGMGG 182
             + + ++            A  +   TR ++A     E   + L +  +  +G+ GMGG
Sbjct: 126 EVEVLRYQGDFAVVAERVDAARVEERPTRPMVAMDPMLESAWNRLMEDEIGILGLHGMGG 185

Query: 183 VGKTKLLDEVKKLVLENNLFHR-------VVVVTVGQLKGATDIQDEVKGKLNMELNENN 242
           VGKT LL  +      NN F R       V+ + V +      IQDE+  KL  + NE  
Sbjct: 186 VGKTTLLSHI------NNRFSRVGGEFDIVIWIVVSKELQIQRIQDEIWEKLRSD-NEKW 245

Query: 243 KEIVE-----------MKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQ 302
           K+  E                ++++DD+W + DL  E G+P    SR+ GCKIV T+R +
Sbjct: 246 KQKTEDIKASNIYNVLKHKRFVLLLDDIWSKVDL-TEVGVP--FPSRENGCKIVFTTRLK 305

Query: 303 NLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE---SMEVGIEQIAKSVVKKCGGLPIALN 362
            +  +M +D   EV  L  +++W  F K VGE        I  +A++V KKC GLP+ALN
Sbjct: 306 EICGRMGVDSDMEVRCLAPDDAWDLFTKKVGEITLGSHPEIPTVARTVAKKCRGLPLALN 365

Query: 363 ILAKTLK-GRNVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGE-AKLLFLMC 422
           ++ +T+   R V+ W+  +  L +  +     + D++   LK  YD++  E  KL F  C
Sbjct: 366 VIGETMAYKRTVQEWRSAIDVLTSS-AAEFSGMEDEILPILKYSYDNLKSEQLKLCFQYC 425

Query: 423 SVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDD 482
           ++FP++H I   DL  Y +G G ++   +N   A ++  +++  L  S LL+E N E   
Sbjct: 426 ALFPEDHNIEKNDLVDYWIGEGFID---RNKGKAENQGYEIIGILVRSCLLMEENQET-- 485

Query: 483 YVRMHDVVRDVAKYIASKIDMHFEPSLMHSLKNLQTLCMLNFTCECNSIDTIGELKTLKI 542
            V+MHDVVR++A +IAS      E  ++ +   LQ+           +I  I + K  + 
Sbjct: 486 -VKMHDVVREMALWIASDFGKQKENFIVQA--GLQS----------RNIPEIEKWKVARR 545

Query: 543 LRIIF--CSTIEELPESMSQLT--------------------ELKVLEVSQCPFLEVIHP 602
           + ++F    +I + PES   +T                     L VL++S    L  + P
Sbjct: 546 VSLMFNNIESIRDAPESPQLITLLLRKNFLGHISSSFFRLMPMLVVLDLSMNRDLRHL-P 605

Query: 603 NIISSMTKLEELSLWESFER-WGEEVSYKNKL----IKNVKLSE----LNGLTRLFSLKL 621
           N IS    L+ LSL  +  R W   +    KL    ++  ++ E    ++GLT L  L+L
Sbjct: 606 NEISECVSLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTRMVESICGISGLTSLKVLRL 663

BLAST of Lcy03g000520 vs. ExPASy Swiss-Prot
Match: Q9LMP6 (Probable disease resistance protein At1g15890 OS=Arabidopsis thaliana OX=3702 GN=At1g15890 PE=3 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 3.8e-36
Identity = 141/476 (29.62%), Postives = 239/476 (50.21%), Query Frame = 0

Query: 159 VGVCGMGGVGKTKLLDEVKKLVLEN-NLFHRVVVVTVGQLKGATDIQDEVKGKLNM---- 218
           +G+ GMGGVGKT LL  +    LE  N F  V+ V V +      IQ+++ G+L +    
Sbjct: 177 LGLYGMGGVGKTTLLASINNKFLEGMNGFDLVIWVVVSKDLQNEGIQEQILGRLGLHRGW 236

Query: 219 -ELNENNK-----EIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRF 278
            ++ E  K      I+ +K  +L++ DDLW E DLE+  G+P    +R+ G KIV T+R 
Sbjct: 237 KQVTEKEKASYICNILNVKKFVLLL-DDLWSEVDLEK-IGVP--PLTRENGSKIVFTTRS 296

Query: 279 QNLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE---SMEVGIEQIAKSVVKKCGGLPIAL 338
           +++ + M +D   +V+ L  +E+W+ F K VG         I  +A+ V +KC GLP+AL
Sbjct: 297 KDVCRDMEVDGEMKVDCLPPDEAWELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLAL 356

Query: 339 NILAKTLKGR-NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSI-DGEAKLLFLM 398
           +++ K +  R  V+ W+ V+  L N  S     + +++   LK  YD + D + KL FL 
Sbjct: 357 SVIGKAMASRETVQEWQHVIHVL-NSSSHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLY 416

Query: 399 CSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERD 458
           CS+FP+++E+  E+L  Y M  G + D  ++ + A ++   ++  L  ++LL++   E  
Sbjct: 417 CSLFPEDYEVRKEELIEYWMCEGFI-DGNEDEDGANNKGHDIIGSLVRAHLLMD--GELT 476

Query: 459 DYVRMHDVVRDVAKYIASKIDMHFEP-------SLMHSLKNLQTLCMLNFTCECNSIDTI 518
             V+MHDV+R++A +IAS      E         L H  K++    +   +  CN I  I
Sbjct: 477 TKVKMHDVIREMALWIASNFGKQKETLCVKPGVQLCHIPKDINWESLRRMSLMCNQIANI 536

Query: 519 G------ELKTLKI----LRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIIS 578
                   L TL +    L  I C     +P        L VL++S+   L  + P  IS
Sbjct: 537 SSSSNSPNLSTLLLQNNKLVHISCDFFRFMP-------ALVVLDLSRNSSLSSL-PEAIS 596

Query: 579 SMTKLEELSLWESFERWGEEVSYKN-KLIKNVKLSELNGLTRLFSLKLQVQNVDML 601
            +  L+ ++L  +  +W   VS+K  K + ++ L   + L  +  +   + N+ +L
Sbjct: 597 KLGSLQYINLSTTGIKW-LPVSFKELKKLIHLNLEFTDELESIVGIATSLPNLQVL 635

BLAST of Lcy03g000520 vs. ExPASy Swiss-Prot
Match: Q9LVT3 (Probable disease resistance protein At5g47260 OS=Arabidopsis thaliana OX=3702 GN=At5g47260 PE=3 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 8.4e-36
Identity = 151/537 (28.12%), Postives = 256/537 (47.67%), Query Frame = 0

Query: 135 QILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEVKKLVLENNLFHRVVVVTV 194
           Q+  ++  L +     L D NV  +G+ G GGVGKT LL +++  +L  + F  V+ V V
Sbjct: 148 QLTVSQAKLLDTAWARLMDINVGTLGIYGRGGVGKTTLLTKLRNKLLV-DAFGLVIFVVV 207

Query: 195 GQLKGATDIQDEVKGKLNMELNENNKE-------IVEMKDNILIMVDDLWKECDLEREFG 254
           G  +    IQDE+  +L ++     KE        V  +   ++++D + +E DLE E G
Sbjct: 208 G-FEEVESIQDEIGKRLGLQWRRETKERKAAEILAVLKEKRFVLLLDGIQRELDLE-EIG 267

Query: 255 IPCRSESRKEGCKIVITSRFQNLLKQMN-IDKWFEVNSLNEEESWKFFVKIVGES---ME 314
           +P    SR  GCKIV T++      +   +D   E+  L+ EE+W  F + VGE+     
Sbjct: 268 VP--FPSRDNGCKIVFTTQSLEACDESKWVDAKVEITCLSPEEAWDLFQETVGENTLRSH 327

Query: 315 VGIEQIAKSVVKKCGGLPIALNILAKTLKG-RNVRVWKDVLKKLKNPLSVNIRDVSDQLY 374
             I ++A+ V   C GLP+ALN++ + + G R VR W+  +  L +  +    D+ D   
Sbjct: 328 QDIPKLARVVASTCRGLPLALNLIGEAMSGKRTVREWRYTIHVLASS-TAEFPDMEDGTL 387

Query: 375 TCLKICYDSIDGE-AKLLFLMCSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRV 434
             LK  YD++  E  +L FL C++FP+N +I  EDL  Y +  G+L  + +  E+A  + 
Sbjct: 388 PILKSIYDNMSDEIIRLCFLYCALFPENLDIGKEDLVNYWICEGILAKEDR--EEAEIQG 447

Query: 435 MKLVDDLKTSYLLLESNNERDDYVRMHDVVRDVAKYIASKIDMHFEPSLMHSLKNLQTLC 494
            +++ DL    LL+ES N   + V+MH +VR++A +IAS+  +      +H + N+    
Sbjct: 448 YEIICDLVRMRLLMESGN--GNCVKMHGMVREMALWIASEHFVVVGGERIHQMLNVNDWR 507

Query: 495 M-------------LNFTCECNSIDTI----------------GELKTLKILRIIFCSTI 554
           M             ++ + +C+ + T+                  +  L +L + F   +
Sbjct: 508 MIRRMSVTSTQIQNISDSPQCSELTTLVFRRNRHLKWISGAFFQWMTGLVVLDLSFNREL 567

Query: 555 EELPESMSQLTELKVLEVS-QC---------PFLEVIH-----------PNIISSMTKLE 609
            ELPE +S L  L+ L +S  C             +IH            ++I+S+  L+
Sbjct: 568 AELPEEVSSLVLLRFLNLSWTCIKGLPLGLKELKSLIHLDLDYTSNLQEVDVIASLLNLQ 627

BLAST of Lcy03g000520 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.9e-224
Identity = 464/861 (53.89%), Postives = 574/861 (66.67%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           M+    +   VV+Y + PIGRQL YLFFY RNIKDLEKQL+ L+  RE+VQ LVNE RSN
Sbjct: 1   MDVFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSN 60

Query: 61  SYEIDNYVLTW-LAEVDGELLEMPQQNE------GSNISCFNCVQWHQLARKTKKR---- 120
           +YEI + V TW L EVD EL  MP+Q E       SN+SC N VQ H+L+RK+KKR    
Sbjct: 61  AYEIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDI 120

Query: 121 ----EYNK--DNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCG 180
               E  K  + VGH APLPDTK  TLPADYQ+L++RTL+AE+IKDAL++PNVNKVGVCG
Sbjct: 121 LQLIEEGKKFEKVGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCG 180

Query: 181 MGGVGKTKLLDEVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNEN----- 240
           M GVGKTKLLDE+KKLVLEN LF RV+ VTVG+  G  +IQD++ GKLNM L+       
Sbjct: 181 MAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKE 240

Query: 241 ------NKEIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL- 300
                   ++VEMKD ILIM+DDLW E DLE+E GIPCRSES KEGCKI++TSR +++L 
Sbjct: 241 GRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDILT 300

Query: 301 KQMNIDKWFEVNSLNEEESWKFFVKIVGESMEVGIEQIAKSVVKKCGGLPIALNILAKTL 360
            +MN  K F+VNSL+E+ESWKFF+ IVGE  +  IE IAK+VVKKCGGLPIAL I+AK L
Sbjct: 301 NKMNTKKCFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKAL 360

Query: 361 KGRNVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSI-DGEAKLLFLMCSVFPDNH 420
           KG+ + +WKD  +KL+  + V I+ VSDQLY+CLK+ YD I D E +LLFL+CSVFPD+H
Sbjct: 361 KGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDH 420

Query: 421 EISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDV 480
            I V+DLQMY+MGMGLLN DI NWE  ++RV+ LVDDLK+SYLLLESN+  D+YV+MHDV
Sbjct: 421 NICVKDLQMYAMGMGLLN-DINNWEATKNRVIDLVDDLKSSYLLLESNS-GDNYVQMHDV 480

Query: 481 VRDVAKYIASKID----------------------------------------------- 540
           VRDVAKYIAS  D                                               
Sbjct: 481 VRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQL 540

Query: 541 -----------------------------------MHFEP---SLMHSLKNLQTLCMLNF 600
                                              M FEP   S+ HSLKNLQTLCM   
Sbjct: 541 LILGMPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTLCM--S 600

Query: 601 TCECNSIDTIGELKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIIS 660
            CE   IDTIG+LK LKIL+I  C T EELP +MSQLT+L++L++S C  L+VI  NIIS
Sbjct: 601 YCEYYDIDTIGQLKKLKILKINDC-TFEELPANMSQLTQLRLLDLSNCSNLKVIPINIIS 660

Query: 661 SMTKLEELSLWESFERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELS 720
           SMTKLEEL+LW SF+RWGEE+SY+NKLI+NVKLSELN L+RL +L+L++ NV++ S++LS
Sbjct: 661 SMTKLEELNLWRSFKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVNIFSDDLS 720

Query: 721 LERAQNLEEFMIRAG--YYDTCLSPKYATSLFLDIESKIDSIGGVLEILLQRCEQLGASE 745
           LER + LEEF I  G   +   +   YA  L ++I+S+I +IGG L+ILL+ CEQL   +
Sbjct: 721 LERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGCEQLYIED 780

BLAST of Lcy03g000520 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 2.1e-162
Identity = 370/805 (45.96%), Postives = 501/805 (62.24%), Query Frame = 0

Query: 7   IVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSNSYEIDN 66
           I+  V+   I PIG QLGYL  Y RN K+L +QL+ L+  ++ V   V E +  SY I  
Sbjct: 3   ILVSVIAATIKPIGHQLGYLVCYNRNKKELREQLENLETTKKDVNQRVEEAKGKSYTISE 62

Query: 67  YVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKKRE------YNKDN----V 126
            V  WLA+VD  +      N  SN SCFN  Q +QL+RK +K+        NK N    V
Sbjct: 63  EVSKWLADVDNAITHDELSN--SNPSCFNLAQRYQLSRKREKQVNYILQLMNKRNSFVEV 122

Query: 127 GHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEV 186
           G+RAPLPDT+   +P DYQ+L+++TLLA+ IK+AL+ P VNK+GV GM GVGKT  L+EV
Sbjct: 123 GYRAPLPDTENTVVPGDYQVLESKTLLAKDIKNALSKPEVNKIGVYGMAGVGKTYFLNEV 182

Query: 187 KKLVL--ENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNENNK--------EIVEMK 246
           KKLVL  E+ LF RV+ V VG+    TDIQ+++  +LN+EL ++ +         + +M+
Sbjct: 183 KKLVLKGEDRLFDRVIDVRVGRFNDVTDIQEQIGDQLNVELPKSKEGRASFLRNNLAKME 242

Query: 247 DNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL-KQMNIDKWFEVNSL 306
            NILI++DDLWKE DL +E GIP      K+GCK++ITSR Q++L   MN  + F+V+SL
Sbjct: 243 GNILILLDDLWKEYDLLKEIGIPL----SKDGCKVLITSRSQDILTNNMNTQECFQVSSL 302

Query: 307 NEEESWKFFVKIVGESME-VGIEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVWKDVLK 366
           +EEESWKFF+ I+G+  + +  + IAK+V K+CGGLP+AL+ +AK LKG+++  W+D L 
Sbjct: 303 SEEESWKFFMAIIGDKFDTIYKKNIAKNVAKECGGLPLALDTIAKALKGKDMHHWEDALT 362

Query: 367 KLKNPLSVNIRDVSDQLYTCLKICYDSIDG-EAKLLFLMCSVFPDNHEISVEDLQMYSMG 426
           KL+N + ++I+ VSD++Y  L++ YD +DG E KL+FL+CSVFPD+++IS+++LQMY+M 
Sbjct: 363 KLRNSIGMDIKGVSDKVYASLRLSYDHLDGEETKLIFLLCSVFPDDYKISIKNLQMYAMC 422

Query: 427 MGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNE-RDDYVRMHDVVRDVAKYIASK- 486
           M LLN  +K WED+++RVMKLV+DL +S LLLE+ ++ +D YV+MHDVVRDVA +IASK 
Sbjct: 423 MRLLN-KVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHDVVRDVAIHIASKE 482

Query: 487 ---------------------------------------IDMHF---------------E 546
                                                  + M+F               E
Sbjct: 483 GNMSTLNIGYNKVNEWEDECRSGSHRAIFANCDNLNNLPLKMNFPQLELLILRVSYWLVE 542

Query: 547 PSLM-----------------------------HSLKNLQTLCMLNFTCECNSIDTIGEL 606
            +L                               SL NLQ LCML   CE N IDTIGEL
Sbjct: 543 DNLQIPYAFFDGMVKLKVLDLTGMCCLRPLWTTPSLNNLQALCMLR--CEFNDIDTIGEL 602

Query: 607 KTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWES 666
           K L++LRI+ C+ ++ LP +MSQLT LKVLEV  CP LEV+  NI SSMTKLEEL L +S
Sbjct: 603 KKLEVLRIVKCNMLDHLPPTMSQLTHLKVLEVLNCPKLEVVPANIFSSMTKLEELKLQDS 662

Query: 667 FERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMIR 697
           F RWGEEV YK++L+KNV +SELN L  L +L L+  NV +LS E+S +  + L+EF I 
Sbjct: 663 FCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILS-EISSQTCKKLKEFWIC 722

BLAST of Lcy03g000520 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 3.3e-160
Identity = 372/832 (44.71%), Postives = 507/832 (60.94%), Query Frame = 0

Query: 7   IVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSNSYEIDN 66
           I+  V+   I PIG QLGYL  Y RN K+L  QL+ L+  +  V   V E +  SY I  
Sbjct: 3   ILVSVIAATIKPIGHQLGYLVCYNRNKKELRDQLENLETTKRDVNQRVQEAKGKSYTISE 62

Query: 67  YVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKKRE------YNKDN----V 126
            V  WLA+VD  +L     N  SN SCFN  + +QL+RK +K+        NK N    V
Sbjct: 63  EVSKWLADVDNAILHDELSN--SNPSCFNLARRYQLSRKREKQVNYILQLMNKRNSFVEV 122

Query: 127 GHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEV 186
           G+RAPLPDT+   +P DYQ+L+++T LA+ IK+ALA P V KVGV GM GVGKT LL+EV
Sbjct: 123 GYRAPLPDTENIVVPGDYQVLESKTSLAKDIKNALAKPEVKKVGVYGMAGVGKTYLLNEV 182

Query: 187 KKLVL--ENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNENNK--------EIVEMK 246
           KKLVL  E+ LF R + V VG+    T+IQ+++  +LN+EL ++ +         +V+M+
Sbjct: 183 KKLVLKGEDRLFDRAIDVRVGRFNDVTEIQEQIGDQLNIELPKSKEGRASFLRNNLVKME 242

Query: 247 DNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL-KQMNIDKWFEVNSL 306
            NILI++DDLWKE DL +E GIP      KEGCK+++TSR Q++L   MN  + F+V+SL
Sbjct: 243 GNILILLDDLWKEYDLLKEIGIPL----SKEGCKVLMTSRSQDILTNNMNTQECFQVSSL 302

Query: 307 NEEESWKFFVKIVGESME-VGIEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVWKDVLK 366
           +EEESWKFF+ I+G+  + +  + IAK+V K+CGGLP+AL+ +AK LKG+++  W+D L 
Sbjct: 303 SEEESWKFFMAIIGDKFDTIYKKNIAKNVAKECGGLPLALDTIAKALKGKDMHHWEDALT 362

Query: 367 KLKNPLSVNIRDVSDQLYTCLKICYDSIDG-EAKLLFLMCSVFPDNHEISVEDLQMYSMG 426
           KL++ + ++I+ VSD++Y  L++ Y+ +DG E KLLFL+CSVF D+++I ++DLQMY+MG
Sbjct: 363 KLRDSIGMDIKGVSDKVYASLRLSYEHLDGEETKLLFLLCSVFADDYKIPIKDLQMYAMG 422

Query: 427 MGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNE-RDDYVRMHDVVRDVAKYIASK- 486
           M LLN  +K WED+++RVMKLV+DL +S LLLE+ ++ +D YV+MH+VVRDVA +IASK 
Sbjct: 423 MRLLN-KVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHNVVRDVAIHIASKE 482

Query: 487 ---------------------------------------IDMHFEPSL------------ 546
                                                  + M+F P L            
Sbjct: 483 GNMSTFNIGYNKVNEWEDEYRSGSHRAIFANCDNFNNLPLKMNF-PQLELLILRVSNWLV 542

Query: 547 ---------------------------------MHSLKNLQTLCMLNFTCECNSIDTIGE 606
                                              SL NL+TLCML   CE N IDTIGE
Sbjct: 543 ENNLQIPYAFFDGMEKLKVLDLTGMCCLRPLWTTPSLNNLRTLCMLR--CEFNDIDTIGE 602

Query: 607 LKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWE 666
           LK L++LRI+ C+ ++ LP +MSQLT+LKVLEV  C  LEV+  N+ SSMTKLEEL L +
Sbjct: 603 LKKLEVLRIVKCNMLDHLPPTMSQLTQLKVLEVLNCSKLEVVPANVFSSMTKLEELKLQD 662

Query: 667 SFERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMI 724
           SF RWGEEV YK++L+KNV +SELN L  L +L L+  NV +LS E++ +  + L+EF I
Sbjct: 663 SFCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILS-EINSQTCKKLKEFWI 722

BLAST of Lcy03g000520 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 7.4e-160
Identity = 371/727 (51.03%), Postives = 460/727 (63.27%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           M+ L  IV  V +Y + PIGRQLGYLFFY +NIKD+EKQL+ L+  REKVQ LVNE RSN
Sbjct: 1   MDILISIVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSN 60

Query: 61  SYEIDNYVLTW-LAEVDGELLEMPQQN------EGSNISCFNCVQWHQLARKTKKR---- 120
           +YEI + V TW LAEVD EL  MP +N      +GSN SC N VQ H+L+R++KKR    
Sbjct: 61  AYEIYDEVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDI 120

Query: 121 ----EYNK--DNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCG 180
               E  K  D VG+ APLPDTK  TLPADYQIL++RTLLAE+IKD+LADPNVNKVGVCG
Sbjct: 121 LEIIEKGKKFDKVGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCG 180

Query: 181 MGGVGKTKLLDEVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNEN----- 240
           MGGVGKTKLLDEVKKLVLEN LF RV+ VT+GQ  G  +IQD++ GKLNM LN       
Sbjct: 181 MGGVGKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKE 240

Query: 241 ------NKEIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL- 300
                   ++VEMKD+ILIM+DDLW E DL++E GIPCRS S KEGCKI++TSR +++L 
Sbjct: 241 GRAPFLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCRSRSSKEGCKILMTSRSRDILT 300

Query: 301 KQMNIDKWFEVNSLNEEESWKFFVKIVGESMEVGIEQIAKSVVKKCGGLPIALNILAKTL 360
            +M+ +K FEVNSL+EEESWKFF+ IVGE  +  IE +A++VVKKCGGLPIAL I+AK L
Sbjct: 301 NRMDAEKCFEVNSLSEEESWKFFIAIVGEFDKNRIEHLARNVVKKCGGLPIALKIIAKAL 360

Query: 361 KGRNVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSI-DGEAKLLFLMCSVFPDNH 420
           KG++VR+WKD L+KL+  +SV I+ VSDQLY+CLK+ YD I D E +LLFL+CSVFPD+H
Sbjct: 361 KGKDVRIWKDALEKLEKRVSVKIKGVSDQLYSCLKLSYDRIEDEETRLLFLLCSVFPDDH 420

Query: 421 EISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDV 480
           +I V+DLQMY+MGMGLLN DI NWE +R+ V++LVD LK+S LLLESN+  D+YV+MHDV
Sbjct: 421 KIHVKDLQMYAMGMGLLN-DIDNWETSRNMVVELVDHLKSSSLLLESNS-GDNYVQMHDV 480

Query: 481 VRDVAKYIASK-----------------------------ID------------------ 540
           VRDVAKYIA+K                             ID                  
Sbjct: 481 VRDVAKYIATKENKMSSLSYGRGQSEWREKERCGSYNVIFIDCVDFPNLPPNMEFPDLQM 540

Query: 541 -----------------------------------MHFEP---SLMHSLKNLQTLCMLNF 600
                                              M FEP   S  HSLKNLQTL M   
Sbjct: 541 LILRIQSFFGEGRAIQIPEALFEGMKKLRVLDMTKMCFEPSKTSFTHSLKNLQTLRM--S 600

Query: 601 TCECNSIDTIGELKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVI-HPNII 609
            CECN I TI +LK L+ILRI     I  L  S  +L +L+    S+   LE + H N +
Sbjct: 601 YCECNDILTIFQLKKLEILRINGIMII-TLQVSFPELEDLR----SEGLSLETLWHNNGL 660

BLAST of Lcy03g000520 vs. ExPASy TrEMBL
Match: A0A6J1FY21 (probable disease resistance protein At4g27220 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448887 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 6.3e-151
Identity = 342/795 (43.02%), Postives = 473/795 (59.50%), Query Frame = 0

Query: 1   METLTIIVEPVV-KYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRS 60
           ME L  + E ++ +Y+I PIGRQL YL  Y RN   L++QL+ L+  R  VQ  V E RS
Sbjct: 1   MEILVSVAESIMGEYVIKPIGRQLSYLLCYNRNKNKLKEQLEALKTARRDVQGRVQEARS 60

Query: 61  NSYEIDNYVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKK----------R 120
           N+Y I   V  WL + D  + +    N  SN S FN V  +QL RKTKK          +
Sbjct: 61  NAYAIREEVSKWLTDADNAIHDELSSN-SSNPSAFNLVDRYQLGRKTKKKIEALHELIAK 120

Query: 121 EYNKDNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGK 180
             N   VG+ APL +TK   +P  YQ+L+++  +A+QIK AL    V++VGV GMGGVGK
Sbjct: 121 RINFVEVGYPAPLLETKNIIVPGGYQVLESKISMAKQIKKALQKAEVSRVGVYGMGGVGK 180

Query: 181 TKLLDEVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNENNK--------E 240
           T LL+EVKK+VLE  +F RV+ V VGQ  G   IQ+++   LN+ L ++ +         
Sbjct: 181 TYLLNEVKKMVLEEKVFDRVIEVCVGQSNGVIQIQEQIGDVLNVRLPKSKEGRASFIRNN 240

Query: 241 IVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQN-LLKQMNIDKWF 300
           +V+M+ ++LI++DDLWKE DL +E GIPC S      CK+++TSR Q+ L+  MN    F
Sbjct: 241 LVKMEGSVLIILDDLWKEYDLVKEIGIPCSS------CKVLMTSRSQDILMNNMNTTMCF 300

Query: 301 EVNSLNEEESWKFFVKIVGESMEV-GIEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVW 360
           +VNSL+EEESWKFF +I+G+  +   +E +AK VV++CGGLP+AL+ +AK LKG+++  W
Sbjct: 301 QVNSLSEEESWKFFTEIIGDEFDTPDMENLAKMVVRECGGLPLALDTVAKALKGKHMNHW 360

Query: 361 KDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDG-EAKLLFLMCSVFPDNHEISVEDLQ 420
           KD L KL+N + ++I+ VSD++Y  L++ YD + G E KL+FL+CSVFPD+++IS+ DLQ
Sbjct: 361 KDALSKLRNSIGMDIKGVSDKVYASLRLSYDHLVGEEPKLMFLLCSVFPDDYKISLNDLQ 420

Query: 421 MYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESN-NERDDYVRMHDVVRDVAKY 480
           MY+MGM +L   +  WED ++RVMKLV DLK+  LLL+++ + RD YV+MHDVVRDVA +
Sbjct: 421 MYAMGMRILR-KVNTWEDMKNRVMKLVHDLKSCSLLLDADEHSRDTYVKMHDVVRDVAIH 480

Query: 481 IASK-------------------------------------------------------- 540
           IAS                                                         
Sbjct: 481 IASMEGIMTTMSFGSKLSDWEDEYRSGAYRAIFVNCDNFQKLPQNLNFPDLELLILRSSN 540

Query: 541 ------------------------IDMHFEPSLMHSLKNLQTLCMLNFTCECNSIDTIGE 600
                                     M F  +   SLKN++TLC+L   CE N IDTIGE
Sbjct: 541 GFVGNLEIPDGFFAGMEKIKALDITGMSFLQTSWRSLKNIRTLCLLR--CEFNHIDTIGE 600

Query: 601 LKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWE 660
           LK L+ILRI  C+ ++ LP +M+QLTELKVLEV  C  LEV   N+ISSM KLE L L +
Sbjct: 601 LKNLEILRICSCNKLDHLPVAMNQLTELKVLEVLNCSNLEVFPANVISSMNKLEVLKLRD 660

Query: 661 SFERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMI 689
           SF+RWGEEV YK++LI N+KLSELN L RL SL+L+  +V +LS E++ E +  L+EF +
Sbjct: 661 SFDRWGEEVGYKDELIMNIKLSELNCLERLSSLRLESSSVKILS-EITSETSNKLKEFWV 720

BLAST of Lcy03g000520 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 788.9 bits (2036), Expect = 3.9e-224
Identity = 464/861 (53.89%), Postives = 574/861 (66.67%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           M+    +   VV+Y + PIGRQL YLFFY RNIKDLEKQL+ L+  RE+VQ LVNE RSN
Sbjct: 1   MDVFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSN 60

Query: 61  SYEIDNYVLTW-LAEVDGELLEMPQQNE------GSNISCFNCVQWHQLARKTKKR---- 120
           +YEI + V TW L EVD EL  MP+Q E       SN+SC N VQ H+L+RK+KKR    
Sbjct: 61  AYEIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDI 120

Query: 121 ----EYNK--DNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCG 180
               E  K  + VGH APLPDTK  TLPADYQ+L++RTL+AE+IKDAL++PNVNKVGVCG
Sbjct: 121 LQLIEEGKKFEKVGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCG 180

Query: 181 MGGVGKTKLLDEVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNEN----- 240
           M GVGKTKLLDE+KKLVLEN LF RV+ VTVG+  G  +IQD++ GKLNM L+       
Sbjct: 181 MAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKE 240

Query: 241 ------NKEIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL- 300
                   ++VEMKD ILIM+DDLW E DLE+E GIPCRSES KEGCKI++TSR +++L 
Sbjct: 241 GRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDILT 300

Query: 301 KQMNIDKWFEVNSLNEEESWKFFVKIVGESMEVGIEQIAKSVVKKCGGLPIALNILAKTL 360
            +MN  K F+VNSL+E+ESWKFF+ IVGE  +  IE IAK+VVKKCGGLPIAL I+AK L
Sbjct: 301 NKMNTKKCFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKAL 360

Query: 361 KGRNVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSI-DGEAKLLFLMCSVFPDNH 420
           KG+ + +WKD  +KL+  + V I+ VSDQLY+CLK+ YD I D E +LLFL+CSVFPD+H
Sbjct: 361 KGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDH 420

Query: 421 EISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDV 480
            I V+DLQMY+MGMGLLN DI NWE  ++RV+ LVDDLK+SYLLLESN+  D+YV+MHDV
Sbjct: 421 NICVKDLQMYAMGMGLLN-DINNWEATKNRVIDLVDDLKSSYLLLESNS-GDNYVQMHDV 480

Query: 481 VRDVAKYIASKID----------------------------------------------- 540
           VRDVAKYIAS  D                                               
Sbjct: 481 VRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQL 540

Query: 541 -----------------------------------MHFEP---SLMHSLKNLQTLCMLNF 600
                                              M FEP   S+ HSLKNLQTLCM   
Sbjct: 541 LILGMPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTLCM--S 600

Query: 601 TCECNSIDTIGELKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIIS 660
            CE   IDTIG+LK LKIL+I  C T EELP +MSQLT+L++L++S C  L+VI  NIIS
Sbjct: 601 YCEYYDIDTIGQLKKLKILKINDC-TFEELPANMSQLTQLRLLDLSNCSNLKVIPINIIS 660

Query: 661 SMTKLEELSLWESFERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELS 720
           SMTKLEEL+LW SF+RWGEE+SY+NKLI+NVKLSELN L+RL +L+L++ NV++ S++LS
Sbjct: 661 SMTKLEELNLWRSFKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVNIFSDDLS 720

Query: 721 LERAQNLEEFMIRAG--YYDTCLSPKYATSLFLDIESKIDSIGGVLEILLQRCEQLGASE 745
           LER + LEEF I  G   +   +   YA  L ++I+S+I +IGG L+ILL+ CEQL   +
Sbjct: 721 LERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGCEQLYIED 780

BLAST of Lcy03g000520 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 732.3 bits (1889), Expect = 4.4e-207
Identity = 439/857 (51.23%), Postives = 538/857 (62.78%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           ++ +  +V  +  Y I PI  QL YLF YK NIK++EK+L  L+     VQ LVN+ +  
Sbjct: 70  LKEMDAVVGVLADYTIKPILHQLDYLFHYKGNIKEVEKKLGILRTTERTVQILVNQDKGK 129

Query: 61  SYEIDNYVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKK------------ 120
              I + V  WL  V   +L+M QQNE  N SCFNCVQ HQL+RK KK            
Sbjct: 130 GNAIFDGVTNWLDNVK-NVLDMAQQNEAPNPSCFNCVQRHQLSRKAKKRLEDINELINEG 189

Query: 121 REYNKDNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVG 180
           R++NKDNVG+  P PDT   TLPADYQ  ++RT +AE+IKDALA+PNVNKVGVCGMGGVG
Sbjct: 190 RQFNKDNVGYPVPSPDTSSSTLPADYQFFESRTSMAEKIKDALANPNVNKVGVCGMGGVG 249

Query: 181 KTKLLDEVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNME-LNENNKE------ 240
           KT LL EVKKLVLEN LF RV+ V VGQ K    IQ+E++ KLNME LN   +E      
Sbjct: 250 KTTLLKEVKKLVLENKLFDRVIQVEVGQSKSVIHIQEEIRAKLNMEYLNMEFEEVRTSRL 309

Query: 241 ---IVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL-KQMNID 300
              I E K+NIL M+DDLWKE D+E+EFGIP RS+SR EGCKI++TSR ++L   QMNI+
Sbjct: 310 QTHIAEKKENILFMLDDLWKEYDVEKEFGIPYRSDSRIEGCKILMTSRRRDLFTNQMNIE 369

Query: 301 KWFEVNSLNEEESWKFFVKIVG-ESMEVG-IEQIAKSVVKKCGGLPIALNILAKTLKGRN 360
           K FEVNSL +EESWKFF  I+G E +E G ++QIAK V+ +CGGLPIAL IL KTLKGR 
Sbjct: 370 KLFEVNSLTKEESWKFFSNIIGDEFVEDGYMKQIAKYVINECGGLPIALKILGKTLKGRR 429

Query: 361 VRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGEAKLLFLMCSVFPDNHEISVE 420
           V +WKD LKKLKNP+ V+I  VSDQLY+CL+  YDSI+ EAKLL L+CSVFPD+H I V+
Sbjct: 430 VEIWKDALKKLKNPVVVDIHGVSDQLYSCLQFSYDSIENEAKLLLLLCSVFPDDHRIYVD 489

Query: 421 DLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDVVRDVA 480
           DLQ Y+MGMGLLN     WEDA++RV+KLVDDLK+SYLLL      D+YV+MHDVV D A
Sbjct: 490 DLQKYAMGMGLLN-QTNTWEDAKNRVIKLVDDLKSSYLLLAPPEWDDNYVKMHDVVHDFA 549

Query: 481 KYIASKID---------------------------------------------------- 540
           KYIASKID                                                    
Sbjct: 550 KYIASKIDKMTSFTYGNGQRAWEEDNRHGSYNAIYVDPTTFLNLPPKLEFPNLQLFILIS 609

Query: 541 -----------------------------MHFEPSLMHSLKNLQTLCMLNFTCECNSIDT 600
                                         +FEPSL++SLKNL+ LC+ +  C+ N ID 
Sbjct: 610 NGLGPRPTIQNLETVFEGMKELRVLDVRMWYFEPSLIYSLKNLRVLCLSH--CDSNDIDA 669

Query: 601 IGELKTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELS 660
           IGELK LKIL I FC  I+E PE  S+LT+LKVL+VS C  L+VIHPNIIS MT+LE+LS
Sbjct: 670 IGELKRLKILEIHFCRGIQEFPECTSKLTQLKVLQVSHCMNLKVIHPNIISKMTRLEDLS 729

Query: 661 LWESFERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKL-QVQNVDMLSNELSLERAQNLE 720
              SF+ WGEEV YK KLI+N KLSELN LT L  L+L  ++NV++LSNELSLE  Q LE
Sbjct: 730 FSHSFDGWGEEVWYKQKLIRNAKLSELNCLTHLVHLELCNLKNVEILSNELSLESTQKLE 789

Query: 721 EFMIRAG------YYDTCLSP------KYATSLFLDIESKIDSIGGVLEILLQRCEQLGA 738
              I  G      +Y+   +P      +Y T+L L I+S IDSIGGV +ILLQ+CE+L  
Sbjct: 790 TCRIFVGDERWNIHYNDRNNPYNRKDKRYTTALNLYIDSPIDSIGGVFQILLQKCEKLVL 849

BLAST of Lcy03g000520 vs. NCBI nr
Match: XP_038887227.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 681.4 bits (1757), Expect = 8.9e-192
Identity = 396/797 (49.69%), Postives = 506/797 (63.49%), Query Frame = 0

Query: 7   IVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSNSYEIDN 66
           +V  VV+Y I PI  QLGYLF Y  N+K L+++ K L+  R+ VQ LV++ +     I +
Sbjct: 4   VVGVVVEYTIRPILHQLGYLFRYNGNVKKLQEKHKTLETTRDTVQILVSQDKGKGNAIFD 63

Query: 67  YVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKK------------REYNKD 126
            V  WL  V  E+  M +QN+  N SCFN V+ H+L+RK+KK            R +NKD
Sbjct: 64  EVTKWLDNVK-EVSNMTKQNKELNPSCFNFVKRHRLSRKSKKMVEDIAELINEGRRFNKD 123

Query: 127 NVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLD 186
            +G+  P  DT CPT+P DYQI+++RTLL E IKDALA+PNVNKVGVCGMGGVGKT LL+
Sbjct: 124 RIGYPVPSSDTNCPTIPTDYQIIESRTLLVENIKDALANPNVNKVGVCGMGGVGKTSLLN 183

Query: 187 EVKKLVLENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNE-----NNKEIVEMKDNI 246
           EVKKLVLENNLF RV+ V VGQ K   DIQ+E++G LNM+  +         I+E K+NI
Sbjct: 184 EVKKLVLENNLFDRVIQVEVGQSKSVIDIQEEIRGALNMQCGKITASLLQTHIIERKENI 243

Query: 247 LIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQN-LLKQMNIDKWFEVNSLNEE 306
           L M+DDLWK+ DLE+EFGIPCRSESR EGCKI++TSR ++ L  QMNI+K FEVN + ++
Sbjct: 244 LFMLDDLWKDYDLEKEFGIPCRSESRTEGCKILMTSRSRHTLTNQMNIEKLFEVNPVTKK 303

Query: 307 ESWKFFVKIVGESMEVG-IEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVWKDVLKKLK 366
           ESW FF  IVGE +E G I+QIAK VV +CGGLPIAL ++AK LKG+ V +WKD LKKLK
Sbjct: 304 ESWMFFTAIVGEYVEDGYIQQIAKDVVNECGGLPIALKVIAKALKGKRVEIWKDALKKLK 363

Query: 367 NPLSVNIRDVSDQLYTCLKICYDSIDGEAKLLFLMCSVFPDNHEISVEDLQMYSMGMGLL 426
           NP+ VNI  VSD+LY+CL+  YDSI+ EAKL+ L+CSVFPD+H I V+DLQMY++GMG L
Sbjct: 364 NPVVVNISGVSDRLYSCLQFSYDSIEDEAKLVLLLCSVFPDDHRIYVKDLQMYAVGMGFL 423

Query: 427 NDDIKNWEDARDRVMKLVDDLKTSYLLLESNNER-DDYVRMHDVVRDVAKYIASKID--- 486
           N  I  WEDAR+RV+KLVDDLK+S LLL  + E  ++YV++HDV+ D  KYIASK D   
Sbjct: 424 N-HINTWEDARNRVIKLVDDLKSSNLLLAPDEEEGEEYVKIHDVIHDFGKYIASKTDKMS 483

Query: 487 ------------------------------------------------------------ 546
                                                                       
Sbjct: 484 SLTYRSGQKELQENDRHGTYNAIYLNCSEFLNYPQKLELPNLQLLILRNVLLDYKVIPIP 543

Query: 547 -MHFE--------------------PSLMHSLKNLQTLCMLNFTCECNSIDTIGELKTLK 606
              FE                     SL  SLKNLQTLC+ N  C    IDTI +LK L+
Sbjct: 544 VAFFEGMNGVRVLDVRVAHLGQPQLTSLTQSLKNLQTLCLSN--CGSIDIDTISQLKKLE 603

Query: 607 ILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWESFERW 666
           ILRI  C  I+E+  +MS+LT+LKVL+ + C  L+VIHPN+I++MT+LEEL+L   F+RW
Sbjct: 604 ILRIHHC-IIKEVTVTMSELTQLKVLDFANCSSLKVIHPNVIANMTRLEELNLETDFKRW 663

Query: 667 GEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMIRAGYY 699
           GE VSYK KLIKN +LSELN LT L  L + + N+  LSNELS+ER QNLE+F I  G  
Sbjct: 664 GENVSYKKKLIKNAELSELNCLTHLLYLNVTIPNIHTLSNELSIERVQNLEKFCIHVGAG 723

BLAST of Lcy03g000520 vs. NCBI nr
Match: XP_011650062.1 (probable disease resistance protein At5g63020 [Cucumis sativus] >KAE8652346.1 hypothetical protein Csa_022345 [Cucumis sativus])

HSP 1 Score: 582.8 bits (1501), Expect = 4.3e-162
Identity = 370/805 (45.96%), Postives = 501/805 (62.24%), Query Frame = 0

Query: 7   IVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSNSYEIDN 66
           I+  V+   I PIG QLGYL  Y RN K+L +QL+ L+  ++ V   V E +  SY I  
Sbjct: 3   ILVSVIAATIKPIGHQLGYLVCYNRNKKELREQLENLETTKKDVNQRVEEAKGKSYTISE 62

Query: 67  YVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKKRE------YNKDN----V 126
            V  WLA+VD  +      N  SN SCFN  Q +QL+RK +K+        NK N    V
Sbjct: 63  EVSKWLADVDNAITHDELSN--SNPSCFNLAQRYQLSRKREKQVNYILQLMNKRNSFVEV 122

Query: 127 GHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEV 186
           G+RAPLPDT+   +P DYQ+L+++TLLA+ IK+AL+ P VNK+GV GM GVGKT  L+EV
Sbjct: 123 GYRAPLPDTENTVVPGDYQVLESKTLLAKDIKNALSKPEVNKIGVYGMAGVGKTYFLNEV 182

Query: 187 KKLVL--ENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNENNK--------EIVEMK 246
           KKLVL  E+ LF RV+ V VG+    TDIQ+++  +LN+EL ++ +         + +M+
Sbjct: 183 KKLVLKGEDRLFDRVIDVRVGRFNDVTDIQEQIGDQLNVELPKSKEGRASFLRNNLAKME 242

Query: 247 DNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL-KQMNIDKWFEVNSL 306
            NILI++DDLWKE DL +E GIP      K+GCK++ITSR Q++L   MN  + F+V+SL
Sbjct: 243 GNILILLDDLWKEYDLLKEIGIPL----SKDGCKVLITSRSQDILTNNMNTQECFQVSSL 302

Query: 307 NEEESWKFFVKIVGESME-VGIEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVWKDVLK 366
           +EEESWKFF+ I+G+  + +  + IAK+V K+CGGLP+AL+ +AK LKG+++  W+D L 
Sbjct: 303 SEEESWKFFMAIIGDKFDTIYKKNIAKNVAKECGGLPLALDTIAKALKGKDMHHWEDALT 362

Query: 367 KLKNPLSVNIRDVSDQLYTCLKICYDSIDG-EAKLLFLMCSVFPDNHEISVEDLQMYSMG 426
           KL+N + ++I+ VSD++Y  L++ YD +DG E KL+FL+CSVFPD+++IS+++LQMY+M 
Sbjct: 363 KLRNSIGMDIKGVSDKVYASLRLSYDHLDGEETKLIFLLCSVFPDDYKISIKNLQMYAMC 422

Query: 427 MGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNE-RDDYVRMHDVVRDVAKYIASK- 486
           M LLN  +K WED+++RVMKLV+DL +S LLLE+ ++ +D YV+MHDVVRDVA +IASK 
Sbjct: 423 MRLLN-KVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHDVVRDVAIHIASKE 482

Query: 487 ---------------------------------------IDMHF---------------E 546
                                                  + M+F               E
Sbjct: 483 GNMSTLNIGYNKVNEWEDECRSGSHRAIFANCDNLNNLPLKMNFPQLELLILRVSYWLVE 542

Query: 547 PSLM-----------------------------HSLKNLQTLCMLNFTCECNSIDTIGEL 606
            +L                               SL NLQ LCML   CE N IDTIGEL
Sbjct: 543 DNLQIPYAFFDGMVKLKVLDLTGMCCLRPLWTTPSLNNLQALCMLR--CEFNDIDTIGEL 602

Query: 607 KTLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWES 666
           K L++LRI+ C+ ++ LP +MSQLT LKVLEV  CP LEV+  NI SSMTKLEEL L +S
Sbjct: 603 KKLEVLRIVKCNMLDHLPPTMSQLTHLKVLEVLNCPKLEVVPANIFSSMTKLEELKLQDS 662

Query: 667 FERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMIR 697
           F RWGEEV YK++L+KNV +SELN L  L +L L+  NV +LS E+S +  + L+EF I 
Sbjct: 663 FCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILS-EISSQTCKKLKEFWIC 722

BLAST of Lcy03g000520 vs. NCBI nr
Match: XP_038902150.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-161
Identity = 372/830 (44.82%), Postives = 510/830 (61.45%), Query Frame = 0

Query: 7   IVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSNSYEIDN 66
           ++  VV   I PIG QLGYL  Y +N K+L++Q++ L+  +  V   V E +  +Y + +
Sbjct: 3   VLVSVVAATIKPIGHQLGYLVCYNKNKKELQEQIENLETTKRDVDKRVEEAKGKAYTVSD 62

Query: 67  YVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKKR---------EYNKD-NV 126
            V  WL +VDG ++    Q   SN SCFN  Q ++L+R+TKK+         + NK   V
Sbjct: 63  EVSKWLTDVDGAMIH--HQLSSSNPSCFNLAQRYRLSRETKKKVNYILQLIDKRNKFLEV 122

Query: 127 GHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEV 186
           G+RAPL DT+   +P DYQ+L+++T LA+ IK+ALA P+VNKVGV GMGGVGKT LL+EV
Sbjct: 123 GYRAPLADTENTIVPGDYQVLESKTSLAKDIKNALAKPDVNKVGVYGMGGVGKTYLLNEV 182

Query: 187 KKLVL--ENNLFHRVVVVTVGQLKGATDIQDEVKGKLNMELNENNK--------EIVEMK 246
           K+LVL  ++  F RV+ V+VG+    T IQ+++  +LN+EL ++ +         + +M+
Sbjct: 183 KRLVLKEDDKSFDRVIDVSVGRSNDLTQIQEQIGDQLNIELPKSKEGRASFLRNNLAKME 242

Query: 247 DNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLL-KQMNIDKWFEVNSL 306
            NIL+++DDLWKE DL +E GIP      KEGCK+++TSR +++L   MN  + F+V+SL
Sbjct: 243 GNILVLLDDLWKEYDLIKEIGIPL----SKEGCKVLMTSRSRDILTNNMNTQECFQVSSL 302

Query: 307 NEEESWKFFVKIVGESME-VGIEQIAKSVVKKCGGLPIALNILAKTLKGRNVRVWKDVLK 366
            EEESWKFF  I+G+  + + +E IAK V K+CGGLP+AL+ +AK LKG+++  W+D L 
Sbjct: 303 TEEESWKFFKAIIGDKFDTIYMESIAKEVSKECGGLPLALDTIAKALKGKDMHYWEDALS 362

Query: 367 KLKNPLSVNIRDVSDQLYTCLKICYDSIDG-EAKLLFLMCSVFPDNHEISVEDLQMYSMG 426
           KLKN + ++I+ VSD++Y  L++ Y+ +DG E KLLFL+CSVFPD+++I ++DLQMY+MG
Sbjct: 363 KLKNSIGMDIKGVSDKVYASLRLSYEYLDGEETKLLFLLCSVFPDDYKIGMKDLQMYAMG 422

Query: 427 MGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNE-RDDYVRMHDVVRDVAKYIASK- 486
           M LLN  +K WEDA++RVMKLV DL +S L LE++++ +D YV+MHDVVRDVA +IAS  
Sbjct: 423 MRLLN-KVKTWEDAKNRVMKLVHDLISSSLFLEADSDSKDKYVKMHDVVRDVAIHIASNE 482

Query: 487 ------------------------------------------------------------ 546
                                                                       
Sbjct: 483 GNMSTLNIGYKLNEWEDEYRSGSHRAIFANCDNLNNLPPKMKFPQLELLILRVSNWLVEN 542

Query: 547 -----------------IDM-----HFEPSLMHSLKNLQTLCMLNFTCECNSIDTIGELK 606
                            +DM     H       SL NL+TLCM+   CE N IDTIGELK
Sbjct: 543 NLQIPYAFFEGMEKLKVLDMTGMCFHRPLWTTSSLNNLRTLCMV--CCEFNDIDTIGELK 602

Query: 607 TLKILRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWESF 666
            L+ILRI+ C+ ++ LP +MSQLT LKVLEV  C  LEV+  NI SSMTKLEEL L +SF
Sbjct: 603 KLEILRIMKCNMLDHLPTTMSQLTHLKVLEVLNCSKLEVVPANIFSSMTKLEELKLQDSF 662

Query: 667 ERWGEEVSYKNKLIKNVKLSELNGLTRLFSLKLQVQNVDMLSNELSLERAQNLEEFMIRA 724
            RWGEEV YK++LIKNVKLSELN L RL +L L+   V +LS E+S +  +NL+EF I +
Sbjct: 663 CRWGEEVWYKDELIKNVKLSELNYLPRLSNLCLESWKVKILS-EISSQTCKNLKEFWICS 722

BLAST of Lcy03g000520 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-44
Identity = 166/576 (28.82%), Postives = 276/576 (47.92%), Query Frame = 0

Query: 34  KDLEKQLK-YLQDIREKVQ--SLVNEQRSNSYEIDNYVLTWLAEVDGELLEMPQQNEGSN 93
           K LE++L+ +L+ + E V    L+ E+RS+          WL++ D E+LE  ++ E   
Sbjct: 35  KSLERKLRIWLRKVEENVPLGELILEKRSS-------CAIWLSDKDVEILEKVKRLEEQG 94

Query: 94  ISCFNCVQWHQLARKTKKREYNKDNVGHRAPLPDTKCPTLPADYQILKTRTLLAEQIKDA 153
                 +  ++ +R+  +R                    L   +   KT   + +++KD 
Sbjct: 95  QDLIKKISVNKSSREIVER-------------------VLGPSFHPQKTALEMLDKLKDC 154

Query: 154 LADPNVNKVGVCGMGGVGKTKLLDEVKKLVLE---NNLFHRVVVVTVGQLKGATDIQDEV 213
           L   NV K+GV GMGGVGKT L+  +   +L+      F  V+ VTV +      +Q ++
Sbjct: 155 LKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVSKDFDLKRVQMDI 214

Query: 214 KGKLNMELNENN---------KEIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEG 273
             +L                 + ++++K N L+++DD+W   DL+ + GIP   E  K+ 
Sbjct: 215 AKRLGKRFTREQMNQLGLTICERLIDLK-NFLLILDDVWHPIDLD-QLGIPLALERSKDS 274

Query: 274 CKIVITSRFQNLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE-SMEVGIEQIAKSVVKKC 333
            K+V+TSR   + +QM  ++  +V  L E+E+W+ F   VGE +    ++ IAK V  +C
Sbjct: 275 -KVVLTSRRLEVCQQMMTNENIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHEC 334

Query: 334 GGLPIALNILAKTLKGR-NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGEA 393
            GLP+A+  + +TL+G+  V VWK  L  LK   S    D  ++++  LK+ YD +    
Sbjct: 335 CGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR--SAPSIDTEEKIFGTLKLSYDFLQDNM 394

Query: 394 KLLFLMCSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLE 453
           K  FL C++FP+++ I V +L MY +  GLL D   ++ED  +  + LV+ LK S LL  
Sbjct: 395 KSCFLFCALFPEDYSIKVSELIMYWVAEGLL-DGQHHYEDMMNEGVTLVERLKDSCLL-- 454

Query: 454 SNNERDDYVRMHDVVRDVAKYIASKIDMHFEPSLMHS-----------LKNLQTLCMLNF 513
            + +  D V+MHDVVRD A +  S     F   +M             + ++Q + ++  
Sbjct: 455 EDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQDKFVSSVQRVSLMAN 514

Query: 514 TCE------CNSIDTI---------------GELKTLKILRIIFCS--TIEELPESMSQL 559
             E         ++T+               G L+    LRI+  S   I  LP+S S L
Sbjct: 515 KLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILDLSGVRIRTLPDSFSNL 574

BLAST of Lcy03g000520 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 170.6 bits (431), Expect = 4.7e-42
Identity = 162/622 (26.05%), Postives = 303/622 (48.71%), Query Frame = 0

Query: 1   METLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREKVQSLVNEQRSN 60
           ME    ++  +++ +      ++     +K N+K L + L+ L +++  +        + 
Sbjct: 1   MECCAPVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHETLLTK 60

Query: 61  SYEIDNYVLTWLAEVDGELLEMPQQNEGSNISCFNCVQWHQLARKTKK-----REYNKDN 120
              +   ++ W  E + E++   +      +SC   ++  +++RK  K     +   KD 
Sbjct: 61  DKPLRLKLMRWQREAE-EVISKARLKLEERVSCGMSLR-PRMSRKLVKILDEVKMLEKDG 120

Query: 121 VG-------HRAPLPDTKCPTLPADYQILKTRTLLAEQIKDALADPNVNKVGVCGMGGVG 180
           +           P      P +   +Q + +  L   +I+D L      K+GV GMGGVG
Sbjct: 121 IEFVDMLSVESTPERVEHVPGVSVVHQTMASNML--AKIRDGLTSEKAQKIGVWGMGGVG 180

Query: 181 KTKLLDEVKKLVLENNL---FHRVVVVTVGQLKGATDIQDEVKGKLNM--ELNENNKEIV 240
           KT L+  +   + E      F  V+ V V +     ++Q ++  +L++  ++ E+ +++ 
Sbjct: 181 KTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLA 240

Query: 241 E------MKD-NILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQNLLKQMNI 300
                  MK+   L+++DD+WK  DL+   GIP R+E  K G K+++TSRF  + + M  
Sbjct: 241 RRIYVGLMKERKFLLILDDVWKPIDLDL-LGIP-RTEENK-GSKVILTSRFLEVCRSMKT 300

Query: 301 DKWFEVNSLNEEESWKFFVKIVGESMEVG-IEQIAKSVVKKCGGLPIALNILAKTLKG-R 360
           D    V+ L EE++W+ F K  G+ +    + +IAK+V ++CGGLP+A+  +   ++G +
Sbjct: 301 DLDVRVDCLLEEDAWELFCKNAGDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKK 360

Query: 361 NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGEAKLLFLMCSVFPDNHEISV 420
           NV++W  VL KL   +   I+ + ++++  LK+ YD ++ +AK  FL+C++FP+++ I V
Sbjct: 361 NVKLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLEDKAKFCFLLCALFPEDYSIEV 420

Query: 421 EDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDDYVRMHDVVRDV 480
            ++  Y M  G + +++ + ED+ +  +  V+ LK  Y LLE  + RD  V+MHDVVRD 
Sbjct: 421 TEVVRYWMAEGFM-EELGSQEDSMNEGITTVESLK-DYCLLEDGDRRDT-VKMHDVVRDF 480

Query: 481 AKYI--ASKIDMHFEPSLMHSLKNLQTLCMLNFTCECNSIDTI-GELKTLKILRIIFC-S 540
           A +I  +S+ D H   SL+ S   LQ +           +  +  +L++L  L   FC  
Sbjct: 481 AIWIMSSSQDDSH---SLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVK 540

Query: 541 TIEELPESMSQLTELKVLEVSQCPFLEVIHPNIISSMTKLEELSLWESFERWGEEVSYKN 593
           T   L +    L E+ +  +   P L +++     S T+++          +     +  
Sbjct: 541 TSVLLLQGNFLLKEVPIGFLQAFPTLRILN----LSGTRIKSFPSCSLLRLFSLHSLFLR 600

BLAST of Lcy03g000520 vs. TAIR 10
Match: AT5G63020.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 160.6 bits (405), Expect = 4.9e-39
Identity = 183/691 (26.48%), Postives = 315/691 (45.59%), Query Frame = 0

Query: 3   TLTIIVEPVVKYIIVPIGRQLGYLFFYKRNIKDLEKQLKYLQDIREK-VQSLVNEQRSNS 62
           +++I  + + K +   + R   Y+   + N+  L++ L+ ++  RE  ++ +++E+R   
Sbjct: 6   SVSISCDQLTKNVCSCLNRNGDYIHGLEENLTALQRALEQIEQRREDLLRKILSEERRGL 65

Query: 63  YEIDNYVLTWLAEVD------GELLEMPQQNEGSNISCFNC----VQWHQLARKTKKREY 122
             + + V  W+++V+       EL+ M          C  C    V  ++  ++  K   
Sbjct: 66  QRL-SVVQGWVSKVEAIVPRVNELVRMRSVQVQRLCLCGFCSKNLVSSYRYGKRVMKMIE 125

Query: 123 NKDNVGHRAPLPDTKCPTLPADYQILKTRTLLA-----EQIKDALADPNVNKVGVCGMGG 182
             + + ++            A  +   TR ++A     E   + L +  +  +G+ GMGG
Sbjct: 126 EVEVLRYQGDFAVVAERVDAARVEERPTRPMVAMDPMLESAWNRLMEDEIGILGLHGMGG 185

Query: 183 VGKTKLLDEVKKLVLENNLFHR-------VVVVTVGQLKGATDIQDEVKGKLNMELNENN 242
           VGKT LL  +      NN F R       V+ + V +      IQDE+  KL  + NE  
Sbjct: 186 VGKTTLLSHI------NNRFSRVGGEFDIVIWIVVSKELQIQRIQDEIWEKLRSD-NEKW 245

Query: 243 KEIVE-----------MKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRFQ 302
           K+  E                ++++DD+W + DL  E G+P    SR+ GCKIV T+R +
Sbjct: 246 KQKTEDIKASNIYNVLKHKRFVLLLDDIWSKVDL-TEVGVP--FPSRENGCKIVFTTRLK 305

Query: 303 NLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE---SMEVGIEQIAKSVVKKCGGLPIALN 362
            +  +M +D   EV  L  +++W  F K VGE        I  +A++V KKC GLP+ALN
Sbjct: 306 EICGRMGVDSDMEVRCLAPDDAWDLFTKKVGEITLGSHPEIPTVARTVAKKCRGLPLALN 365

Query: 363 ILAKTLK-GRNVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSIDGE-AKLLFLMC 422
           ++ +T+   R V+ W+  +  L +  +     + D++   LK  YD++  E  KL F  C
Sbjct: 366 VIGETMAYKRTVQEWRSAIDVLTSS-AAEFSGMEDEILPILKYSYDNLKSEQLKLCFQYC 425

Query: 423 SVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERDD 482
           ++FP++H I   DL  Y +G G ++   +N   A ++  +++  L  S LL+E N E   
Sbjct: 426 ALFPEDHNIEKNDLVDYWIGEGFID---RNKGKAENQGYEIIGILVRSCLLMEENQET-- 485

Query: 483 YVRMHDVVRDVAKYIASKIDMHFEPSLMHSLKNLQTLCMLNFTCECNSIDTIGELKTLKI 542
            V+MHDVVR++A +IAS      E  ++ +   LQ+           +I  I + K  + 
Sbjct: 486 -VKMHDVVREMALWIASDFGKQKENFIVQA--GLQS----------RNIPEIEKWKVARR 545

Query: 543 LRIIF--CSTIEELPESMSQLT--------------------ELKVLEVSQCPFLEVIHP 602
           + ++F    +I + PES   +T                     L VL++S    L  + P
Sbjct: 546 VSLMFNNIESIRDAPESPQLITLLLRKNFLGHISSSFFRLMPMLVVLDLSMNRDLRHL-P 605

Query: 603 NIISSMTKLEELSLWESFER-WGEEVSYKNKL----IKNVKLSE----LNGLTRLFSLKL 621
           N IS    L+ LSL  +  R W   +    KL    ++  ++ E    ++GLT L  L+L
Sbjct: 606 NEISECVSLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTRMVESICGISGLTSLKVLRL 663

BLAST of Lcy03g000520 vs. TAIR 10
Match: AT1G15890.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 154.8 bits (390), Expect = 2.7e-37
Identity = 141/476 (29.62%), Postives = 239/476 (50.21%), Query Frame = 0

Query: 159 VGVCGMGGVGKTKLLDEVKKLVLEN-NLFHRVVVVTVGQLKGATDIQDEVKGKLNM---- 218
           +G+ GMGGVGKT LL  +    LE  N F  V+ V V +      IQ+++ G+L +    
Sbjct: 177 LGLYGMGGVGKTTLLASINNKFLEGMNGFDLVIWVVVSKDLQNEGIQEQILGRLGLHRGW 236

Query: 219 -ELNENNK-----EIVEMKDNILIMVDDLWKECDLEREFGIPCRSESRKEGCKIVITSRF 278
            ++ E  K      I+ +K  +L++ DDLW E DLE+  G+P    +R+ G KIV T+R 
Sbjct: 237 KQVTEKEKASYICNILNVKKFVLLL-DDLWSEVDLEK-IGVP--PLTRENGSKIVFTTRS 296

Query: 279 QNLLKQMNIDKWFEVNSLNEEESWKFFVKIVGE---SMEVGIEQIAKSVVKKCGGLPIAL 338
           +++ + M +D   +V+ L  +E+W+ F K VG         I  +A+ V +KC GLP+AL
Sbjct: 297 KDVCRDMEVDGEMKVDCLPPDEAWELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLAL 356

Query: 339 NILAKTLKGR-NVRVWKDVLKKLKNPLSVNIRDVSDQLYTCLKICYDSI-DGEAKLLFLM 398
           +++ K +  R  V+ W+ V+  L N  S     + +++   LK  YD + D + KL FL 
Sbjct: 357 SVIGKAMASRETVQEWQHVIHVL-NSSSHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLY 416

Query: 399 CSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRVMKLVDDLKTSYLLLESNNERD 458
           CS+FP+++E+  E+L  Y M  G + D  ++ + A ++   ++  L  ++LL++   E  
Sbjct: 417 CSLFPEDYEVRKEELIEYWMCEGFI-DGNEDEDGANNKGHDIIGSLVRAHLLMD--GELT 476

Query: 459 DYVRMHDVVRDVAKYIASKIDMHFEP-------SLMHSLKNLQTLCMLNFTCECNSIDTI 518
             V+MHDV+R++A +IAS      E         L H  K++    +   +  CN I  I
Sbjct: 477 TKVKMHDVIREMALWIASNFGKQKETLCVKPGVQLCHIPKDINWESLRRMSLMCNQIANI 536

Query: 519 G------ELKTLKI----LRIIFCSTIEELPESMSQLTELKVLEVSQCPFLEVIHPNIIS 578
                   L TL +    L  I C     +P        L VL++S+   L  + P  IS
Sbjct: 537 SSSSNSPNLSTLLLQNNKLVHISCDFFRFMP-------ALVVLDLSRNSSLSSL-PEAIS 596

Query: 579 SMTKLEELSLWESFERWGEEVSYKN-KLIKNVKLSELNGLTRLFSLKLQVQNVDML 601
            +  L+ ++L  +  +W   VS+K  K + ++ L   + L  +  +   + N+ +L
Sbjct: 597 KLGSLQYINLSTTGIKW-LPVSFKELKKLIHLNLEFTDELESIVGIATSLPNLQVL 635

BLAST of Lcy03g000520 vs. TAIR 10
Match: AT5G47260.1 (ATP binding;GTP binding;nucleotide binding;nucleoside-triphosphatases )

HSP 1 Score: 153.7 bits (387), Expect = 6.0e-37
Identity = 151/537 (28.12%), Postives = 256/537 (47.67%), Query Frame = 0

Query: 135 QILKTRTLLAEQIKDALADPNVNKVGVCGMGGVGKTKLLDEVKKLVLENNLFHRVVVVTV 194
           Q+  ++  L +     L D NV  +G+ G GGVGKT LL +++  +L  + F  V+ V V
Sbjct: 148 QLTVSQAKLLDTAWARLMDINVGTLGIYGRGGVGKTTLLTKLRNKLLV-DAFGLVIFVVV 207

Query: 195 GQLKGATDIQDEVKGKLNMELNENNKE-------IVEMKDNILIMVDDLWKECDLEREFG 254
           G  +    IQDE+  +L ++     KE        V  +   ++++D + +E DLE E G
Sbjct: 208 G-FEEVESIQDEIGKRLGLQWRRETKERKAAEILAVLKEKRFVLLLDGIQRELDLE-EIG 267

Query: 255 IPCRSESRKEGCKIVITSRFQNLLKQMN-IDKWFEVNSLNEEESWKFFVKIVGES---ME 314
           +P    SR  GCKIV T++      +   +D   E+  L+ EE+W  F + VGE+     
Sbjct: 268 VP--FPSRDNGCKIVFTTQSLEACDESKWVDAKVEITCLSPEEAWDLFQETVGENTLRSH 327

Query: 315 VGIEQIAKSVVKKCGGLPIALNILAKTLKG-RNVRVWKDVLKKLKNPLSVNIRDVSDQLY 374
             I ++A+ V   C GLP+ALN++ + + G R VR W+  +  L +  +    D+ D   
Sbjct: 328 QDIPKLARVVASTCRGLPLALNLIGEAMSGKRTVREWRYTIHVLASS-TAEFPDMEDGTL 387

Query: 375 TCLKICYDSIDGE-AKLLFLMCSVFPDNHEISVEDLQMYSMGMGLLNDDIKNWEDARDRV 434
             LK  YD++  E  +L FL C++FP+N +I  EDL  Y +  G+L  + +  E+A  + 
Sbjct: 388 PILKSIYDNMSDEIIRLCFLYCALFPENLDIGKEDLVNYWICEGILAKEDR--EEAEIQG 447

Query: 435 MKLVDDLKTSYLLLESNNERDDYVRMHDVVRDVAKYIASKIDMHFEPSLMHSLKNLQTLC 494
            +++ DL    LL+ES N   + V+MH +VR++A +IAS+  +      +H + N+    
Sbjct: 448 YEIICDLVRMRLLMESGN--GNCVKMHGMVREMALWIASEHFVVVGGERIHQMLNVNDWR 507

Query: 495 M-------------LNFTCECNSIDTI----------------GELKTLKILRIIFCSTI 554
           M             ++ + +C+ + T+                  +  L +L + F   +
Sbjct: 508 MIRRMSVTSTQIQNISDSPQCSELTTLVFRRNRHLKWISGAFFQWMTGLVVLDLSFNREL 567

Query: 555 EELPESMSQLTELKVLEVS-QC---------PFLEVIH-----------PNIISSMTKLE 609
            ELPE +S L  L+ L +S  C             +IH            ++I+S+  L+
Sbjct: 568 AELPEEVSSLVLLRFLNLSWTCIKGLPLGLKELKSLIHLDLDYTSNLQEVDVIASLLNLQ 627