Lcy02g018670 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g018670 vs. ExPASy Swiss-Prot
Match: Q08353 (NF-kappa-B inhibitor alpha OS=Sus scrofa OX=9823 GN=NFKBIA PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.9e-09
Identity = 48/166 (28.92%), Postives = 72/166 (43.37%), Query Frame = 0

Query: 49  TALHLAVVANQEEIVETLVRLICENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIA 108
           T LHLAV+ NQ EI E L+   C+         E+++ R N PLHLA   G +     + 
Sbjct: 113 TPLHLAVITNQPEIAEALLEAGCDP--------ELRDFRGNTPLHLACEQGCLASVGVLT 172

Query: 109 SAHG-----GLLDDRNIDDQTPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNNGD 168
              G      +L   N +  T L LA+++G       L S   D      +N +   NG 
Sbjct: 173 QPRGTQHLHSILQATNYNGHTCLHLASIHGYLGIVELLVSLGAD------VNAQEPCNGR 232

Query: 169 TVLHCAIGNEYFDLAFQLMHLNVDSMNWANEHGRTPLHILASKPAS 210
           T LH A+  +  DL   L+    D +N     G +P  +   +P++
Sbjct: 233 TALHLAVDLQNPDLVSLLLKCGAD-VNRVTYQGYSPYQLTWGRPST 263

BLAST of Lcy02g018670 vs. ExPASy Swiss-Prot
Match: A2CIR5 (Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 GN=NPR4 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-08
Identity = 65/245 (26.53%), Postives = 111/245 (45.31%), Query Frame = 0

Query: 359 SEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEK--NVVHLAAEYRQWKLYKF 418
           ++D  ++++  AA+ G VEIVK L E DP+     R++D+K    +H+A +     + + 
Sbjct: 220 AKDNGKNSLHFAARQGHVEIVKALLEKDPQL---ARRNDKKGQTALHMAVKGTNCDVLRA 279

Query: 419 LLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQ--------IQLEVEWYK 478
           L++   ++  L    D NGN+ALH+A  +   K  +I    L+        +  + +   
Sbjct: 280 LVDADPAIVML---PDKNGNTALHVATRK---KRAEIVAVLLRLPDTHVNALTRDHKTAY 339

Query: 479 FVKESLPI-----HFFPLYNNKG---------------ETASRIFEDTHMDLEQKGK--- 538
            + E+LP+         + +  G               +T + I +D H  LEQ  K   
Sbjct: 340 DIAEALPLCEESSEIKDILSQHGALRSRELNQPRDELRKTVTEIKKDVHTQLEQTRKTNK 399

Query: 539 --------------DWLCVTSQSCSVVATLIATVAFETAATIPGGNNDHGLATLKQEQAF 557
                         + +   + S +VVA L ATVAF    T+PGGN ++G+A + Q  +F
Sbjct: 400 NVHGIAKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGNANNGVAVVVQAASF 455

BLAST of Lcy02g018670 vs. ExPASy Swiss-Prot
Match: Q91974 (NF-kappa-B inhibitor alpha OS=Gallus gallus OX=9031 GN=NFKBIA PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.9e-08
Identity = 48/168 (28.57%), Postives = 71/168 (42.26%), Query Frame = 0

Query: 36  PEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLICENPGHNLERLEIKNDRQNNPLHLA 95
           P A   +LT +GDT LHLA++  ++ +   ++R    +       L  +N+    PLHLA
Sbjct: 67  PHAWAQQLTEDGDTFLHLAIIHEEKALSLEVIRQAAGDAAF----LNFQNNLSQTPLHLA 126

Query: 96  AAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAALYGKRDAFYCLYSFCGDDPVRITIN 155
                  +   +  A G  LD R+    TPL +A   G   +   L   C   P  +   
Sbjct: 127 VITDQAEIAEHLLKA-GCDLDVRDFRGNTPLHIACQQGSLRSVSVLTQHC--QPHHLLAV 186

Query: 156 CRLKN-NGDTVLHCAIGNEYFDLAFQLMHLNVDSMNWANEHGRTPLHI 203
            +  N NG T LH A    Y  +   L+ L  D       +GRT LH+
Sbjct: 187 LQATNYNGHTCLHLASIQGYLAVVEYLLSLGADVNAQEPCNGRTALHL 227

BLAST of Lcy02g018670 vs. ExPASy Swiss-Prot
Match: Q25338 (Delta-latroinsectotoxin-Lt1a OS=Latrodectus tredecimguttatus OX=6925 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 7.2e-08
Identity = 53/207 (25.60%), Postives = 87/207 (42.03%), Query Frame = 0

Query: 2   NPTSNNNNNELREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEE 61
           +P    N  E   F   HT   +   V+     HP+      +  G T  HLA++   +E
Sbjct: 593 SPNIKVNEKEDDGFTPLHTAVMSTYMVVDALLNHPDIDKNAQSTSGLTPFHLAIINESQE 652

Query: 62  IVETLVRLICENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNID 121
           + E+LV    +        L I++     P+H AA+MGS++M   + S    +  +   +
Sbjct: 653 VAESLVESNAD--------LNIQDVNHMAPIHFAASMGSIKMLRYLISIKDKVSINSVTE 712

Query: 122 DQ--TPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAF 181
           +   TPL  A  + K DA   L      D + +TI   + +   TVLH A+     ++  
Sbjct: 713 NNNWTPLHFAIYFKKEDAAKELLK---QDDINLTI---VADGNLTVLHLAVSTGQINIIK 772

Query: 182 QLMHLNVDSMNWANEHGRTPLHILASK 207
           +L+    +      E G T LHI A +
Sbjct: 773 ELLKRGSNIEEKTGE-GYTSLHIAAMR 784

BLAST of Lcy02g018670 vs. ExPASy Swiss-Prot
Match: P25963 (NF-kappa-B inhibitor alpha OS=Homo sapiens OX=9606 GN=NFKBIA PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.1e-07
Identity = 47/161 (29.19%), Postives = 72/161 (44.72%), Query Frame = 0

Query: 42  KLTPEGDTALHLAVVANQEEIVETLVRLICENPGHNLERLEIKNDRQNNPLHLAAAMGSV 101
           +LT +GD+ LHLA++  ++ +   ++R +      +L  L  +N+ Q  PLHLA      
Sbjct: 69  QLTEDGDSFLHLAIIHEEKALTMEVIRQV----KGDLAFLNFQNNLQQTPLHLAVITNQP 128

Query: 102 RMCHAIASAHGGLLDDRNIDDQTPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNN 161
            +  A+  A G   + R+    TPL LA   G   +   L   C   P   +I      N
Sbjct: 129 EIAEALLGA-GCDPELRDFRGNTPLHLACEQGCLASVGVLTQSC-TTPHLHSILKATNYN 188

Query: 162 GDTVLHCAIGNEYFDLAFQLMHLNVDSMNWANEHGRTPLHI 203
           G T LH A  + Y  +   L+ L  D       +GRT LH+
Sbjct: 189 GHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHL 223

BLAST of Lcy02g018670 vs. ExPASy TrEMBL
Match: A0A6J1K7T7 (uncharacterized protein LOC111491429 OS=Cucurbita maxima OX=3661 GN=LOC111491429 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 4.3e-149
Identity = 304/640 (47.50%), Postives = 395/640 (61.72%), Query Frame = 0

Query: 6   NNNNNELREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVET 65
           N+ N      LY + +R  W  VI++Y ++  A  LKL   GDTALHLAV+ NQEE VET
Sbjct: 2   NDQNTANSRVLYGYARRGQWEEVIKEYKKNQGAQNLKLNRRGDTALHLAVIDNQEETVET 61

Query: 66  LVRLICENPGHNLER---LEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDD 125
           LV+ I E     + +   LE  ++R NNPLHLAA MGSVRMC AIAS H GL+D RN  D
Sbjct: 62  LVKHISEATKDTITKKKVLETTDERGNNPLHLAAMMGSVRMCDAIASKHNGLVDMRNNVD 121

Query: 126 QTPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLM 185
           +TPLFLAA YG +DA +CLY+FC D+  RI+ NCR+  NGDTVLH A+ NE+FDLAFQL+
Sbjct: 122 ETPLFLAAAYGNKDACFCLYNFCSDNASRISANCRVSANGDTVLHHALRNEHFDLAFQLI 181

Query: 186 HLNVDSMNWANEHGRTPLHILASKPASFKSGSNIRGWRNLVYHFSTLV------------ 245
           +++ ++++W NE G TP+H+LASKP SFKSGS+I+GW+++VYH S +             
Sbjct: 182 NMHKEAISWVNEAGLTPIHVLASKPTSFKSGSHIKGWQHIVYHCSFVKPLQPRSIESLKQ 241

Query: 246 -----------------------------------------------------------N 305
                                                                      N
Sbjct: 242 ELDRSVQKAREKASPSPFPVNYRTCIDFYRGMKDIILTVWLFKCHNKNDTKNDGNEKKNN 301

Query: 306 KKKSSDEANKDKDLEKGEKAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTT 365
            K   D+ N+D + +K    E  ++PPT TN P NY+TC+D F I  SAI+ +LG GS  
Sbjct: 302 TKNDGDKRNRDLEEQKPLDTESATDPPT-TNFPVNYTTCIDFFHIAFSAIMIILGFGSIE 361

Query: 366 IRKIREMKQKHTWSVQVMEKLIDPQFVTPDRHGGGRPDYEDSGMESAEA-----PYTYTL 425
           I+KIR+ K+KHTWSVQVMEKL+  +   PD++        DS  ++ EA     PY +  
Sbjct: 362 IKKIRKKKEKHTWSVQVMEKLL--ELAEPDKYAEDGKSPMDSKFQNDEANGVTLPYNFVD 421

Query: 426 EEDKFSTSIGVKSE-------DVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEK 485
            E +FS  I    E       + TE+AMLLAAKNGV+EIVK + E  P  I +TRK D+K
Sbjct: 422 NEVQFSHFIEEPKELEKPQDVEATETAMLLAAKNGVIEIVKGILERFPLAIRDTRK-DKK 481

Query: 486 NVVHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQ 545
           NVV LAAE+RQ  +Y+FLL ++  +Q LFRAVD  GNSALHLAA    PKLW+ITGAALQ
Sbjct: 482 NVVLLAAEHRQPDVYRFLLKKRSEIQNLFRAVDHKGNSALHLAATSIDPKLWRITGAALQ 541

Query: 546 IQLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVA 560
           +Q EV+WY +VK S+P+  FP +N +G+TAS IF++TH +L  KG +WL  TS+S ++VA
Sbjct: 542 MQWEVKWYNYVKSSVPLRVFPHWNKEGKTASAIFQETHKELATKGGEWLYNTSESFTLVA 601

BLAST of Lcy02g018670 vs. ExPASy TrEMBL
Match: A0A6J1K7T7 (uncharacterized protein LOC111491429 OS=Cucurbita maxima OX=3661 GN=LOC111491429 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 9.7e-32
Identity = 83/167 (49.70%), Postives = 108/167 (64.67%), Query Frame = 0

Query: 555  VISSLHNLSHHV-SLHLDRQIRNQRPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQI 614
            V+ ++ N   HV  L L++ I  Q   FR VD  GNSALHLAA     K W I GAALQ+
Sbjct: 1260 VLLAVENRHPHVYQLLLEKNIVKQ-SAFRVVDSQGNSALHLAAKLGDYKPWLIPGAALQM 1319

Query: 615  QLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVAT 674
            Q E++WY++VK S+P +FFP YN   +TA  I  +TH +L + G  WL  TS+SCS+VA 
Sbjct: 1320 QWELKWYQYVKGSMPPNFFPHYNKDRKTARLILSETHSELVENGGKWLTSTSESCSLVAA 1379

Query: 675  LIATVAFETAATIPGGNNDHGLATLKQEQAFSIFVISSLHNLSHHVS 721
            LIATVAF TAAT+PGGN+  G   L  +  F++F I+SL  LS  V+
Sbjct: 1380 LIATVAFATAATVPGGNDPKGTPLLHGQPTFNVFAIASLIALSCSVT 1425

HSP 2 Score: 534.3 bits (1375), Expect = 8.2e-148
Identity = 306/637 (48.04%), Postives = 394/637 (61.85%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSI------GVKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVH 491
           + S SI        K ++V E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNVV 
Sbjct: 454 RLSNSIESKPKEAEKPKNVQETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNVVL 513

Query: 492 LAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQLE 551
           LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q E
Sbjct: 514 LAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQWE 573

Query: 552 VEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATLIA 560
           ++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TLI 
Sbjct: 574 IKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLIV 633

BLAST of Lcy02g018670 vs. ExPASy TrEMBL
Match: A0A6J1K4G3 (uncharacterized protein LOC111491104 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491104 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 5.3e-147
Identity = 305/639 (47.73%), Postives = 393/639 (61.50%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSIGVKSED--------VTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNV 491
           + S SI  K ++          E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNV
Sbjct: 454 RLSNSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNV 513

Query: 492 VHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQ 551
           V LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q
Sbjct: 514 VLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQ 573

Query: 552 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 560
            E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TL
Sbjct: 574 WEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTL 633

BLAST of Lcy02g018670 vs. ExPASy TrEMBL
Match: A0A6J1JZF8 (uncharacterized protein LOC111491104 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491104 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 5.3e-147
Identity = 305/639 (47.73%), Postives = 393/639 (61.50%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSIGVKSED--------VTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNV 491
           + S SI  K ++          E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNV
Sbjct: 454 RLSNSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNV 513

Query: 492 VHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQ 551
           V LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q
Sbjct: 514 VLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQ 573

Query: 552 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 560
            E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TL
Sbjct: 574 WEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTL 633

BLAST of Lcy02g018670 vs. ExPASy TrEMBL
Match: A0A6J1EX58 (uncharacterized protein LOC111437338 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437338 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 4.5e-146
Identity = 305/649 (47.00%), Postives = 394/649 (60.71%), Query Frame = 0

Query: 5   SNNNNNE--LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEI 64
           S N  +E  LR+FLY +TKR  W  VI+KY +HPEA GLKLT  GDTALHLAV+ N+EE+
Sbjct: 25  SMNEKHEATLRDFLYINTKRTEWEKVIKKYEKHPEAQGLKLTRNGDTALHLAVLDNREEM 84

Query: 65  VETLVRLICENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDD 124
           V+ LV  I ++    L  LE  NDR+ NPLHLAA MGS  MC+AIASAH  L++ RN  D
Sbjct: 85  VQKLVNRIKDSKRDEL--LETTNDRKENPLHLAAQMGSATMCYAIASAHHKLVEKRNKID 144

Query: 125 QTPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLM 184
           +TPL+LAA  G RDAF+CLY FC D    IT NCRL +NGDTVLH A+ N++FDLAF ++
Sbjct: 145 ETPLYLAAASGNRDAFFCLYHFCRDLDSGITANCRLSSNGDTVLHSALRNDHFDLAFHIL 204

Query: 185 HLNVDSMNWANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH----------------- 244
           HL+ ++M+W  + G TPLH+LASKP +FKSGS IRGWRN+ Y+                 
Sbjct: 205 HLHNEAMHWVTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLNPQPIDSLIR 264

Query: 245 -------------------------FSTLV--------------------NKKKSSDEAN 304
                                    F T V                      KK +D+A 
Sbjct: 265 DWIDRMSNPNTSTPCFPANYETCIDFFTWVWDGFLKGSGLKRICHDFKNDESKKDTDDAG 324

Query: 305 KDKDLEKGE----------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITV 364
           ++  +E GE                KA  ++   +ITN P NY+TC+  FQI+ SAI+  
Sbjct: 325 RNIMVEGGESSEAAEPHQRLDTQLLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAILIS 384

Query: 365 LGHGSTTIRKIREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEAP 424
           LG GS   +KIR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + A   
Sbjct: 385 LGWGSAEFKKIRRQKEKHTWSVQVMEKLL--EYAAPDEYDCNGGIPMDSTSQTPDQAGVT 444

Query: 425 YTYTLEEDKFSTSIGVKS----------EDVTESAMLLAAKNGVVEIVKELYECDPRTIY 484
             Y+ ++D    S+ ++S          +D  E+ MLLAAKNGV+EIVK ++   P +IY
Sbjct: 445 LPYSFQDDDVLFSVHIESKPTEAEKPKPKDFQETPMLLAAKNGVIEIVKGMFCRFPLSIY 504

Query: 485 ETRKSDEKNVVHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLW 544
           +  K D+KNVV LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W
Sbjct: 505 DAGK-DKKNVVLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIW 564

Query: 545 QITGAALQIQLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVT 560
           +ITGAALQ+Q E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL QK  DWL  T
Sbjct: 565 RITGAALQMQWEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGDWLIKT 624

BLAST of Lcy02g018670 vs. NCBI nr
Match: XP_022996109.1 (uncharacterized protein LOC111491429 [Cucurbita maxima])

HSP 1 Score: 538.5 bits (1386), Expect = 9.0e-149
Identity = 304/640 (47.50%), Postives = 395/640 (61.72%), Query Frame = 0

Query: 6   NNNNNELREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVET 65
           N+ N      LY + +R  W  VI++Y ++  A  LKL   GDTALHLAV+ NQEE VET
Sbjct: 2   NDQNTANSRVLYGYARRGQWEEVIKEYKKNQGAQNLKLNRRGDTALHLAVIDNQEETVET 61

Query: 66  LVRLICENPGHNLER---LEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDD 125
           LV+ I E     + +   LE  ++R NNPLHLAA MGSVRMC AIAS H GL+D RN  D
Sbjct: 62  LVKHISEATKDTITKKKVLETTDERGNNPLHLAAMMGSVRMCDAIASKHNGLVDMRNNVD 121

Query: 126 QTPLFLAALYGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLM 185
           +TPLFLAA YG +DA +CLY+FC D+  RI+ NCR+  NGDTVLH A+ NE+FDLAFQL+
Sbjct: 122 ETPLFLAAAYGNKDACFCLYNFCSDNASRISANCRVSANGDTVLHHALRNEHFDLAFQLI 181

Query: 186 HLNVDSMNWANEHGRTPLHILASKPASFKSGSNIRGWRNLVYHFSTLV------------ 245
           +++ ++++W NE G TP+H+LASKP SFKSGS+I+GW+++VYH S +             
Sbjct: 182 NMHKEAISWVNEAGLTPIHVLASKPTSFKSGSHIKGWQHIVYHCSFVKPLQPRSIESLKQ 241

Query: 246 -----------------------------------------------------------N 305
                                                                      N
Sbjct: 242 ELDRSVQKAREKASPSPFPVNYRTCIDFYRGMKDIILTVWLFKCHNKNDTKNDGNEKKNN 301

Query: 306 KKKSSDEANKDKDLEKGEKAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTT 365
            K   D+ N+D + +K    E  ++PPT TN P NY+TC+D F I  SAI+ +LG GS  
Sbjct: 302 TKNDGDKRNRDLEEQKPLDTESATDPPT-TNFPVNYTTCIDFFHIAFSAIMIILGFGSIE 361

Query: 366 IRKIREMKQKHTWSVQVMEKLIDPQFVTPDRHGGGRPDYEDSGMESAEA-----PYTYTL 425
           I+KIR+ K+KHTWSVQVMEKL+  +   PD++        DS  ++ EA     PY +  
Sbjct: 362 IKKIRKKKEKHTWSVQVMEKLL--ELAEPDKYAEDGKSPMDSKFQNDEANGVTLPYNFVD 421

Query: 426 EEDKFSTSIGVKSE-------DVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEK 485
            E +FS  I    E       + TE+AMLLAAKNGV+EIVK + E  P  I +TRK D+K
Sbjct: 422 NEVQFSHFIEEPKELEKPQDVEATETAMLLAAKNGVIEIVKGILERFPLAIRDTRK-DKK 481

Query: 486 NVVHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQ 545
           NVV LAAE+RQ  +Y+FLL ++  +Q LFRAVD  GNSALHLAA    PKLW+ITGAALQ
Sbjct: 482 NVVLLAAEHRQPDVYRFLLKKRSEIQNLFRAVDHKGNSALHLAATSIDPKLWRITGAALQ 541

Query: 546 IQLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVA 560
           +Q EV+WY +VK S+P+  FP +N +G+TAS IF++TH +L  KG +WL  TS+S ++VA
Sbjct: 542 MQWEVKWYNYVKSSVPLRVFPHWNKEGKTASAIFQETHKELATKGGEWLYNTSESFTLVA 601

BLAST of Lcy02g018670 vs. NCBI nr
Match: XP_022996109.1 (uncharacterized protein LOC111491429 [Cucurbita maxima])

HSP 1 Score: 148.7 bits (374), Expect = 2.0e-31
Identity = 83/167 (49.70%), Postives = 108/167 (64.67%), Query Frame = 0

Query: 555  VISSLHNLSHHV-SLHLDRQIRNQRPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQI 614
            V+ ++ N   HV  L L++ I  Q   FR VD  GNSALHLAA     K W I GAALQ+
Sbjct: 1260 VLLAVENRHPHVYQLLLEKNIVKQ-SAFRVVDSQGNSALHLAAKLGDYKPWLIPGAALQM 1319

Query: 615  QLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVAT 674
            Q E++WY++VK S+P +FFP YN   +TA  I  +TH +L + G  WL  TS+SCS+VA 
Sbjct: 1320 QWELKWYQYVKGSMPPNFFPHYNKDRKTARLILSETHSELVENGGKWLTSTSESCSLVAA 1379

Query: 675  LIATVAFETAATIPGGNNDHGLATLKQEQAFSIFVISSLHNLSHHVS 721
            LIATVAF TAAT+PGGN+  G   L  +  F++F I+SL  LS  V+
Sbjct: 1380 LIATVAFATAATVPGGNDPKGTPLLHGQPTFNVFAIASLIALSCSVT 1425

HSP 2 Score: 534.3 bits (1375), Expect = 1.7e-147
Identity = 306/637 (48.04%), Postives = 394/637 (61.85%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSI------GVKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVH 491
           + S SI        K ++V E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNVV 
Sbjct: 454 RLSNSIESKPKEAEKPKNVQETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNVVL 513

Query: 492 LAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQLE 551
           LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q E
Sbjct: 514 LAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQWE 573

Query: 552 VEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATLIA 560
           ++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TLI 
Sbjct: 574 IKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLIV 633

BLAST of Lcy02g018670 vs. NCBI nr
Match: KAG6606413.1 (Chlorophyllase type 0, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 533.5 bits (1373), Expect = 2.9e-147
Identity = 302/638 (47.34%), Postives = 389/638 (60.97%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY + KR NW  VI KY +HPEA GLKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 19  LRDFLYINMKRGNWKEVINKYEKHPEAQGLKLTRNGDTALHLAVLDNREEMVQKLVNRIK 78

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
           ++    L  LE  NDR+ NPLHLAA MGS  MC+AIASAH  L+++RN  D+TPL+LAA 
Sbjct: 79  DSKCDKL--LETTNDREENPLHLAAQMGSATMCYAIASAHHKLVEERNKMDETPLYLAAA 138

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC D    IT NCRL +NGDTVLH A+ N++FDLAF ++HLN ++M+W
Sbjct: 139 SGNRDAFFCLYHFCRDLGPEITANCRLSSNGDTVLHSALRNDHFDLAFHILHLNNEAMHW 198

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             + G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 199 VTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVEQLKPQPIDSLIRDWMDRMSNT 258

Query: 252 ----------------FSTLV--------------------NKKKSSDEANKDKDLEKGE 311
                           F T V                      KK +D+A ++  +E GE
Sbjct: 259 NTSTPCFPANYGICIDFFTWVWDGFLKGSGLKRICYDFKNDESKKDTDDAGRNIMVEGGE 318

Query: 312 ----------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIR 371
                           KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +
Sbjct: 319 RSEADEPHQRLDTQLLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAILISLGWGSAEFK 378

Query: 372 KIREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEAPYTYTLEEDK 431
           KIR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + A+    Y+ E+D 
Sbjct: 379 KIRRQKEKHTWSVQVMEKLL--EYAAPDEYDCNGGIPMDSTSQTPDQADVTLPYSFEDDD 438

Query: 432 FSTSIGVKS--------EDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVV 491
              S+ ++S        +D  E+ MLLAAKNGV+EIVK ++   P +IY+  K D+KNVV
Sbjct: 439 VLFSVHIESKPTEAEKPKDFQETPMLLAAKNGVIEIVKGMFRRFPLSIYDAGK-DKKNVV 498

Query: 492 HLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQL 551
            LAAEY Q  +Y+FLL+     + LFRAVD NGNSALHLAAA S   +W+ITGAALQ+Q 
Sbjct: 499 LLAAEYGQPDVYRFLLSPNVYKENLFRAVDANGNSALHLAAAASKSMIWRITGAALQMQW 558

Query: 552 EVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATLI 560
           E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL QK  DWL  TS+SCSVV  LI
Sbjct: 559 EIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGDWLTKTSKSCSVVGALI 618

BLAST of Lcy02g018670 vs. NCBI nr
Match: XP_022995622.1 (uncharacterized protein LOC111491104 isoform X3 [Cucurbita maxima])

HSP 1 Score: 531.6 bits (1368), Expect = 1.1e-146
Identity = 305/639 (47.73%), Postives = 393/639 (61.50%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSIGVKSED--------VTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNV 491
           + S SI  K ++          E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNV
Sbjct: 454 RLSNSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNV 513

Query: 492 VHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQ 551
           V LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q
Sbjct: 514 VLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQ 573

Query: 552 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 560
            E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TL
Sbjct: 574 WEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTL 633

BLAST of Lcy02g018670 vs. NCBI nr
Match: XP_022995620.1 (uncharacterized protein LOC111491104 isoform X1 [Cucurbita maxima])

HSP 1 Score: 531.6 bits (1368), Expect = 1.1e-146
Identity = 305/639 (47.73%), Postives = 393/639 (61.50%), Query Frame = 0

Query: 12  LREFLYAHTKRANWGAVIQKYAQHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLIC 71
           LR+FLY +TKR  W  VI+KY ++PEA  LKLT  GDTALHLAV+ N+EE+V+ LV  I 
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPEAQELKLTRNGDTALHLAVLDNREEVVQKLVNRIK 93

Query: 72  ENPGHNLERLEIKNDRQNNPLHLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAAL 131
               ++ E L+  NDR+  PLHLAA MGS  MC+AIASAH  L+D RN  D+TPL+LAA 
Sbjct: 94  HTSKYD-ELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGKRDAFYCLYSFCGDDPVRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNW 191
            G RDAF+CLY FC ++  RIT NCRL +NGDTVLH A+ N++FDLAFQ++HLN ++M+W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 ANEHGRTPLHILASKPASFKSGSNIRGWRNLVYH-------------------------- 251
             E G TPLH+LASKP +FKSGS IRGWRN+ Y+                          
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 ----------FSTLVN-------------------KKKSSDEANKDKD------LEKGE- 311
                     + T ++                       +DE+ KD        +E GE 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 ---------------KAERISNPPTITNSPENYSTCVDLFQIILSAIITVLGHGSTTIRK 371
                          KA  ++   +ITN P NY+TC+  FQI+ SAI+  LG GS   +K
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IREMKQKHTWSVQVMEKLIDPQFVTPDRH--GGGRP-DYEDSGMESAEA--PYTYTLEED 431
           IR  K+KHTWSVQVMEKL+  ++  PD +   GG P D      + AE   PY+    E 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL--EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEV 453

Query: 432 KFSTSIGVKSED--------VTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNV 491
           + S SI  K ++          E+AMLLAAKNGV+EIVK ++   P +I + RK D+KNV
Sbjct: 454 RLSNSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARK-DKKNV 513

Query: 492 VHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARSSPKLWQITGAALQIQ 551
           V LAAEY Q  +Y+FLL+ K   + LFRAVDDNGNSALHLAAA S   +W+ITGAALQ+Q
Sbjct: 514 VLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQ 573

Query: 552 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 560
            E++WYKFV+ES+P++FF  YN +G+ A+ IF +THMDL +K  +WL  TS+SCSVV TL
Sbjct: 574 WEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTL 633

BLAST of Lcy02g018670 vs. TAIR 10
Match: AT3G18670.1 (Ankyrin repeat family protein )

HSP 1 Score: 135.6 bits (340), Expect = 1.6e-31
Identity = 131/537 (24.39%), Postives = 214/537 (39.85%), Query Frame = 0

Query: 34  QHPEAHGLKLTPEGDTALHLAVVANQEEIVETLVRLICENPGHNLER-LEIKNDRQNNPL 93
           ++PEA    LT  GDT +H AV++   +IVE ++R I     H+ E+ L+IKND     L
Sbjct: 71  RNPEALTAILTSNGDTPIHKAVLSGHIKIVEEIIRRI-----HDPEQVLKIKNDNGYTAL 130

Query: 94  HLAAAMGSVRMCHAIASAHGGLLDDRNIDDQTPLFLAALYGKRDAFYCLYS---FCGDDP 153
             AA  G VR+   + +   GL+  RN  +  P+ +A+LYG +     LYS       DP
Sbjct: 131 TYAATGGIVRIAECLVNKCPGLVSVRNAKEHIPIVVASLYGHKHLVQYLYSHTPLSDLDP 190

Query: 154 VRITINCRLKNNGDTVLHCAIGNEYFDLAFQLMHLNVDSMNWANEHGRTPLHILASKPAS 213
              +   + KN    V +C +   Y  +A  L+          +    T +  LA  P +
Sbjct: 191 CDDSDEHKGKNGAMLVTNCIVDGLYC-IALDLIQRYPKLAYTRDSDNDTAIMALAQTPYA 250

Query: 214 FKSGSNIRGWRNLVYHFSTLVNKKKSSDEANKDKDLEKGEKAERISNPPTITNSPENYST 273
           F S   I                                                     
Sbjct: 251 FPSVPRI----------------------------------------------------- 310

Query: 274 CVDLFQIILSAIITVLGHGSTTIRKIREMKQKHTWSVQVMEKLID--PQFVTPDRHGGGR 333
                                 IR++ ++K  H  + ++++ +    P+F    +   G 
Sbjct: 311 ----------------------IRRVYKLKLGHAQAKEILDCICQEIPKFDAAQQKNAG- 370

Query: 334 PDYEDSGMESAEAPYTYTLEEDKFSTSIGVKSEDVTESAMLLAAKNGVVEIVKELYECDP 393
                                                 A+  A +NG+VE ++E+    P
Sbjct: 371 -----------------------------------LNQALFKAVENGIVEYIEEMMRHYP 430

Query: 394 RTIYETRKSDEKNVVHLAAEYRQWKLYKFLLNRKYSLQPLFRAVDDNGNSALHLAAARS- 453
             ++ ++ S   N+   A   RQ K++  + N       L    D   N+ LH AA R+ 
Sbjct: 431 DIVW-SKNSSGLNIFFYAVSQRQEKIFSLIYNIGAKKNILATNWDIFHNNMLHHAAYRAP 489

Query: 454 SPKLWQITGAALQIQLEVEWYKFVKESL-PIHFFPLYNNKGETASRIFEDTHMDLEQKGK 513
           + +L  I GAALQ+Q E++W+K V++ + P H   +   + +T   +F D H DL ++G+
Sbjct: 491 ASRLNLIPGAALQMQRELQWFKEVEKLVQPKHRKMVNLKQKKTPKALFTDQHKDLVEQGE 489

Query: 514 DWLCVTSQSCSVVATLIATVAFETAATIPGGNNDHGLATLKQEQAFSIFVISSLHNL 563
            W+  T+ SC+VVA LI T+ F +A T+PGG    G+     +  F IF+IS   +L
Sbjct: 551 KWMKETATSCTVVAALITTMMFSSAFTVPGGYRSDGMPLYIHQHRFKIFLISDAISL 489

BLAST of Lcy02g018670 vs. TAIR 10
Match: AT5G04700.1 (Ankyrin repeat family protein )

HSP 1 Score: 115.9 bits (289), Expect = 1.3e-25
Identity = 76/220 (34.55%), Postives = 116/220 (52.73%), Query Frame = 0

Query: 346 LEEDKFSTSIGVKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVHLA 405
           + E+  +  +  +SE V E A+L A + G V+ + E+   +   ++ TR S    +  LA
Sbjct: 343 ISEETLALGLKERSETVDE-ALLFAVRYGNVDFLVEMIRNNSELLWSTRTSSSSTLFLLA 402

Query: 406 AEYRQWKLYKFLL---NRKYSLQPLFRAVDDNGNSALHLAAARSSP-KLWQITGAALQIQ 465
            E+RQ K++  L    +RKY    L    D +GN  LHLA   S P KL  + GA LQ+Q
Sbjct: 403 VEFRQEKVFSLLYGLDDRKYL---LLADKDCDGNGVLHLAGFPSPPSKLSSVVGAPLQLQ 462

Query: 466 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 525
            E++W+K V+   P       N + +T   IF   H  L Q+ + W+  T+ SCS+VA L
Sbjct: 463 RELQWFKEVERIAPEIEKERVNTEEQTPIEIFTKEHQGLRQEAEKWMKDTAMSCSLVAAL 522

Query: 526 IATVAFETAATIPGGNNDH--GLATLKQEQAFSIFVISSL 560
           I TV F    T+PGG +D+  G     +++ F IF++S L
Sbjct: 523 IVTVTFAAVFTVPGGTDDNSKGKPFHLRDRRFIIFIVSDL 558

BLAST of Lcy02g018670 vs. TAIR 10
Match: AT5G04730.1 (Ankyrin-repeat containing protein )

HSP 1 Score: 106.3 bits (264), Expect = 1.1e-22
Identity = 60/203 (29.56%), Postives = 111/203 (54.68%), Query Frame = 0

Query: 357 VKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVHLAAEYRQWKLYKF 416
           ++ ++    A+L AAK+G  +   E+ +C+ + ++    +  +N+  LA E+++ K++  
Sbjct: 288 IRWKETVYEALLEAAKSGNRDFFIEIIKCNSQLLWILNPTSGRNLFQLAVEFKKEKIFNL 347

Query: 417 LLNRKYSLQPLFRAVDDNGNSALHLAAARSSP-KLWQITGAALQIQLEVEWYKFVKESLP 476
           +         L R+ D   N+ LH+A   S+P +L +I+GAAL++Q E +W+K V+  + 
Sbjct: 348 IHGLDDRKVTLLRSYDKGNNNILHIAGRLSTPDQLSKISGAALKMQRESQWFKEVESLVS 407

Query: 477 IHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATLIATVAFETAATIPG 536
                  N   +T  +IFE  H  L ++G++W+  T+ +CS VA LIATV F+   T+PG
Sbjct: 408 EREVVQKNKDNKTPRQIFEHYHEHLRKEGEEWMKYTATACSFVAALIATVTFQAIFTVPG 467

Query: 537 G-NNDHGLATLKQEQAFSIFVIS 558
           G +   G   +  +  F  F+ +
Sbjct: 468 GIDGTSGSPLILNDLHFRAFIFT 490

BLAST of Lcy02g018670 vs. TAIR 10
Match: AT5G04680.1 (Ankyrin repeat family protein )

HSP 1 Score: 102.1 bits (253), Expect = 2.0e-21
Identity = 75/229 (32.75%), Postives = 114/229 (49.78%), Query Frame = 0

Query: 346 LEEDKFSTSIGVKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVHLA 405
           + E+  +  +  +SE V E A+L A + G V+ + E+   +   ++ TR S    +  LA
Sbjct: 359 ISEETLTLGLKERSETVDE-ALLFAVRYGNVDFLVEMIRNNSELLWSTRTSSSSTLFLLA 418

Query: 406 AEYRQWKLYKFLL---NRKYSLQPLFRAVDDNGNSALHLAAARSSP-KLWQITGAALQIQ 465
            E+RQ K++  L    +RKY    L    D +GN  LHLA   S P KL  +  A L++Q
Sbjct: 419 VEFRQEKVFNLLYGLDDRKYL---LLADKDSDGNGVLHLAGFPSPPSKLASVICAPLRMQ 478

Query: 466 LEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVATL 525
            E++W+K V+   P       N + +T   IF   H  L Q+ + W+  T+ SCS+VA L
Sbjct: 479 RELQWFKEVERIAPEIEKERVNTEEQTPIEIFAKEHQGLRQEAEKWMKDTAMSCSLVAAL 538

Query: 526 IA---------TVAFETAATIPGGNNDH--GLATLKQEQAFSIFVISSL 560
           I          TV F    T+ GG++D+  G      EQ F IF++S L
Sbjct: 539 IVMVTFAALIITVIFAAVFTVSGGSDDNSEGNPFHLYEQRFIIFIVSDL 583

BLAST of Lcy02g018670 vs. TAIR 10
Match: AT5G04690.1 (Ankyrin repeat family protein )

HSP 1 Score: 97.8 bits (242), Expect = 3.8e-20
Identity = 71/221 (32.13%), Postives = 115/221 (52.04%), Query Frame = 0

Query: 346 LEEDKFSTSIGVKSEDVTESAMLLAAKNGVVEIVKELYECDPRTIYETRKSDEKNVVHLA 405
           + E+  +  +  +SE V E A+L A + G V+ + E+ + +   ++ T  S    + + A
Sbjct: 304 ISEETLALGLKERSESVDE-ALLFAVRYGNVDFLVEMIKNNSELLWSTGTS---TLFNTA 363

Query: 406 AEYRQWKLYKFLL---NRKYSLQPLFRA-VDDNGNSALHLAA-ARSSPKLWQITGAALQI 465
            + RQ K++  L    +RKY    LF A  D +GNS LHLA     + KL  +  A LQ+
Sbjct: 364 VQVRQEKVFSLLYGLGDRKY----LFLADKDSDGNSVLHLAGYPPPNYKLATVVSATLQM 423

Query: 466 QLEVEWYKFVKESLPIHFFPLYNNKGETASRIFEDTHMDLEQKGKDWLCVTSQSCSVVAT 525
           Q E++W+K ++  +P       N +  T   IF   H  +  + + W+  T+ SCS+VA 
Sbjct: 424 QRELQWFKEMERIVPAIENERVNTENLTPIEIFRKEHEAMRLEAEKWMKDTAMSCSLVAA 483

Query: 526 LIATVAFETAATIPGGNNDH--GLATLKQEQAFSIFVISSL 560
           LI TV F    T+PGG +D+  G    + E+ F IF++S L
Sbjct: 484 LIVTVTFAAIFTVPGGTDDNSGGRPFHRHERIFVIFIVSDL 516