Lcy02g018460 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g018460
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMagnesium transporter
LocationChr02: 45601463 .. 45606063 (-)
RNA-Seq ExpressionLcy02g018460
SyntenyLcy02g018460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGTGATGGATTTGTAATCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGAAGACGACGACGAAGAAGAAGAAGAATACTGCGGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGAGTACGGTTTTGGACGTGGATAAGTATGCGATTATGCAGAGGGTTCAGATTAATGCGAGAGATCTTCGCCTTCTCGATCCTCTCTTGTCGTATCCTTCCACTATCTTGGGCAGAGAACGAGTGATTGTTCTCAATTTGGAGGTAAAGTTACTGGAGATTGGTTCTTCGATCTTGTGAACTTCTGCTGATCTAACTTTGTTGAATTTCGGGATTTTTTTGTGTTATTTTACTGATTTTTTGGTGTCGCTCTGGGAATTGGTCCAGCACATCAAGTCAATTATCACTGCGGACGAGGTAATAATTTATTTATCCGTTTTTTGATTAATTAACTGTCTATTGATTGAGATTCTCTTTAATTGGTTGCTTTGTTGGGTTTATTTAGGCTAATTTTGGTTGAAGTCATTCTATCTTATGATGGGTTTTTCTAATTTTCAAACTCCCTTTTGGGGTTTAATAGTTCCTATAAAGGTTTCAAGTTGACTATGTTAATGGAAGTATTTATGTGTCTAGACTGTTCAAAGGATGCATCACATTGACTAAAATTATAAGTTTGACAAACCAAAATGTAAAAGAGGAGCTAAACTAAGAATTCAAACTCCTTTCCTAGGTTCGTCTTTTTACTATTTGACTTTGAATTTGGAGGAAGTTGACTTTATTTATTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTGTGCTGGGGTTAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAACTGCAGAGACGGTTGCCTTCCACAAGCTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCTCCTACCACCCAAAATGAGTTGGCTGAAAACGGTACAGCTTGGTGAAATATTCTCTCATGGGTTTTCCCCTTATATTCAATGATGCCATATTTGGAAAGATAATCATTATTGCTTGTAAAATTCATAATATTTTACATAGGAGTTAGATATTTCTTTTTCACTTCTAAGTTTTGTCATTCAATGCAGAATTTCCATTCGAGTTCCGCGCCTTAGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAGCTAGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGGTGAAGCTTGTTTAATCCTGCATATCCATTTAGTTGATCCATTCTTGAAATTGGAACTATTTACTGCTTAAACTTAAGTTATATCAGTAAAACAGAGATAAGTTTTCACGGATATATATTGAACAGCCTTCTAATTTCCTTTATTAGTTATATAGAGCATTGGATGTTTTTCTTGAATATCTTTGTCTTTTTAACTTCCTGCTACATCTCAAGTTCTCTAGGCTTGACGATTAACTGAAAGCAATCATTTTTCAGTTATATTTATTTCCATCAAAAGAAACAGAGTTATTTTCATCAAGATAATTATCTTCAAGGTGCTTTTGTGTGTATAATCATTCATACCATCTTATTTTTCTCTAGTAAAGACTTGAAAGGTGAATATCAGATATTGAAATGATCTGTCTCTCTGTAGTCTCTACTAGAAAGCTAATATGGTAGGAACATATAAAAGAATTATATGATTGTATTCTTTCATTTGGAGATTCTATTTTTAGTTTTATAATTATGGATGCGACGTGGAAAGGGACAGGACAAGTCATATATTTAATCAAATTCAATGCAAACTTTTGGTTACAAAACTTATCAAAGTAATACTGCATTCATAATTATGTTCTTAAAAAATCTAAATAAAAAGTTTCATATATGCTTATGGATGAATAATTGGTTGCTAATGACGTTCTTGTAACAATTAAGCTGTATAATAAAATGGATTTTTCCTCAAAATGAACTGACACAGATGTTTACTACCCCTCATCTAATGTTTAATCTGTATTAGGCACCATACCAAATTATCATTGCCTTTACCTGGTTGACCTGTAGTCTCCCTTTCTTCTTCTGAATCTAAGAAATTTGACATTTGACCTGATATGCAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACAAACCGGGTGCAAAAGGTAGTAAATTTTTGGTGCCATCTATAGTATGTGATATGAGGCTATGAGCCATTCTTGGCTTTCACTTATTCACTGCTCTAGGATCTTCAACTAAATATACCCTTTCAATAACAAACATCATAACATAAGGAGGAGGTACAAGCTAACGGCCAAGAGAAGTTGCATTGTACTACTATCTTCAACCAAATTTGTAATTGATCTACTCTAGGATGGTATATAGGTTGAACATCATGATATCTCTGGTTTACAAGTTTGATATTACTTTCAATTTTCAGGAAAAGTTGACAAGACAATAATTTCATAAAGTAAGGGTAGTTTTAGTGTTCAATATTCTGGATCTTCAACTAAAACTGTCAGTACACAAGCCCTTGCTATCCGAGATATATCGATTCAATTTTACTCTTCCAGTCAACATATAGAGCATTCAGTAAACGCCAGTTCACAATTCACCTTTCTCTTTGTGAATATTATCCAACAGCCACATTTTTAAACGTTCCTGATACTGCTCATTTGATCTTAGTTTGGTAGGGACAAAAAAGAGCTAAAAAGCAACAAAATAAATTATCATTGTTGAAATAAGTATGAGGCAACTAGTCCCCTCAAGAACCACACAGGTGCCAAAGAACCACTTGGGCGATTCAATACTTTGGTTTGGGTGGGTTAATCCTCTCCTGTTTGCTTGTCAGAGAACAGAATATGGAAAGATAACTAATAGTAGCCAACTGATGTATTTTTCTCATTCAATGTCGTTAGCTTCTTATTCTTGTTCCAGGTTAGAGATGAACTTGAACAACTTCTCGATGACGATGATGACATGGCGGAACTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGTGGCTGTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAGGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGCGCGATTACAATTCGAGAAGAGAATGATGTTGAGGAACTAGAAATGCTACTTGAGGTATAATCACTGTCTTTTAAAACATGATATATAACATCTAATTTACGGAGACTCCAGTACATTAAGATCTAATTGAGCTTAATTCTGAATATTCTGCAGGCTTATTTTATGCAAATTGAAGGAACATTAAACAAACTAATCACGGTATGAACTGAACTTCAAAAGTGCGTTAATGATAATTGCTATCTGTTCTTATCTGATATTTTGTATGTGGAACTTTCTGATTATGTGGGATTTTTGTATGATATATTTTGCGATCATCTTCACTCATCAGTTGCGGGAGTACATCGATGACACAGAGGATTACATCAATATTCAGGTATTCGTTCCAACATTGTTGACTATTTAATGTTGAGGATCCCACATTGAAAAGATTAGTGGAGACCTCCCAATTTATAAGATGTATGGGTTACTCCACTCATTGCCAATTGGTTTTGAGATGGAAGCCCATGTAATCTAATATTTAAGACTCTCTCATGCCATTAAAATATTGTTTTCTTTGTGATTTCTACTTCTGAATATTCTTGACAATGTTGAAAACTACAATCTACTGTTGTTTCTTATTAAATCCCCCGCTCATCTTTTGCAGCTGGACAACCATAGAAATCAACTTATTCAGGTACTCTTTTTAAAGCCTGAATGGTTTTCTTAAAGAATTTAATTTAAACCTCTGTTTCTTTTGGTTAAAGTTTTTCATCTATTGGCTATTGAAGTGGCCAGACTTTTCACTTAAATTCATCTGGTAGAACTGTATCAAGGTTTTTTCTTTTTAGTTCTCAGAAATGTAATGTTCTATGTGGCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTTTTCAAATGGGTAAGTATTTCTGGTGAAATTGAATGGAAAATCATATCAGAAACTCTCATTCTTTTTGAGACAGTCTCTTCTGAAGCTGCACTGTTGATCTTTTCTTTTCCATGAATTTAGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCCTTTACATGTCGCTAAGCTCGTATGCACGGTACAAAGGTCTCGTTGGATCTTGAACATTCTGCTCGTAAATTGTTTCCACGGTTTCGAGAAATTATGAACTCGAGTATGAAATGATTCTTGAAGTTTGTTGGGGAAGGATATGGAAGAAGGTTTACCACTTTGATATGTTACTAGGAAGCCATTGATAACTTTTGCTTGAGTCAAGAGATTTTCCAGGCATGTATTGTTTATGGCTTATCATTTTTTTTTCACTCTTTGTATTTTATATCATGTACAATTATTGGAAAAGGAATACCAATTAGTGAAAAGATTCATGTCAAAATTAATTACTAAAAAC

mRNA sequence

ATGGCTGGTGATGGATTTGTAATCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGAAGACGACGACGAAGAAGAAGAAGAATACTGCGGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGAGTACGGTTTTGGACGTGGATAAGTATGCGATTATGCAGAGGGTTCAGATTAATGCGAGAGATCTTCGCCTTCTCGATCCTCTCTTGTCGTATCCTTCCACTATCTTGGGCAGAGAACGAGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATTATCACTGCGGACGAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAACTGCAGAGACGGTTGCCTTCCACAAGCTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCTCCTACCACCCAAAATGAGTTGGCTGAAAACGAATTTCCATTCGAGTTCCGCGCCTTAGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAGCTAGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACAAACCGGGTGCAAAAGGTTAGAGATGAACTTGAACAACTTCTCGATGACGATGATGACATGGCGGAACTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGTGGCTGTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAGGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGCGCGATTACAATTCGAGAAGAGAATGATGTTGAGGAACTAGAAATGCTACTTGAGGCTTATTTTATGCAAATTGAAGGAACATTAAACAAACTAATCACGTTGCGGGAGTACATCGATGACACAGAGGATTACATCAATATTCAGCTGGACAACCATAGAAATCAACTTATTCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTTTTCAAATGGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCCTTTACATGTCGCTAAGCTCGTATGCACGGTACAAAGGTCTCGTTGGATCTTGAACATTCTGCTCGTAAATTGTTTCCACGGTTTCGAGAAATTATGAACTCGAGTATGAAATGATTCTTGAAGTTTGTTGGGGAAGGATATGGAAGAAGGTTTACCACTTTGATATGTTACTAGGAAGCCATTGATAACTTTTGCTTGAGTCAAGAGATTTTCCAGGCATGTATTGTTTATGGCTTATCATTTTTTTTTCACTCTTTGTATTTTATATCATGTACAATTATTGGAAAAGGAATACCAATTAGTGAAAAGATTCATGTCAAAATTAATTACTAAAAAC

Coding sequence (CDS)

ATGGCTGGTGATGGATTTGTAATCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGAAGACGACGACGAAGAAGAAGAAGAATACTGCGGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGAGTACGGTTTTGGACGTGGATAAGTATGCGATTATGCAGAGGGTTCAGATTAATGCGAGAGATCTTCGCCTTCTCGATCCTCTCTTGTCGTATCCTTCCACTATCTTGGGCAGAGAACGAGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATTATCACTGCGGACGAGGTGCTGCTTAGAGATCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAACTGCAGAGACGGTTGCCTTCCACAAGCTCCATTTACCAAGGACAAGGTGAAGAAGAAGAGCCTCCTACCACCCAAAATGAGTTGGCTGAAAACGAATTTCCATTCGAGTTCCGCGCCTTAGAGGTTGCACTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAGCTAGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGATTAGCAGTCGTAACTTGGACCGAGTGCGCAAACTGAAGAGTGCAATGACAAGGTTGACAAACCGGGTGCAAAAGGTTAGAGATGAACTTGAACAACTTCTCGATGACGATGATGACATGGCGGAACTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGTGGCTGTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAGGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGCGCGATTACAATTCGAGAAGAGAATGATGTTGAGGAACTAGAAATGCTACTTGAGGCTTATTTTATGCAAATTGAAGGAACATTAAACAAACTAATCACGTTGCGGGAGTACATCGATGACACAGAGGATTACATCAATATTCAGCTGGACAACCATAGAAATCAACTTATTCAGCTAGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCAATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTTTTCAAATGGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCCTTTACATGTCGCTAAGCTCGTATGCACGGTACAAAGGTCTCGTTGGATCTTGA

Protein sequence

MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS
Homology
BLAST of Lcy02g018460 vs. ExPASy Swiss-Prot
Match: B8AJT9 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.9e-144
Identity = 274/373 (73.46%), Postives = 310/373 (83.11%), Query Frame = 0

Query: 28  KNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRERVIV 87
           K    SRSW+  D  G   VLD DKYAIM RV INARDLR+LDPLLSYPSTILGRER IV
Sbjct: 20  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 79

Query: 88  LNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTSSIYQGQGEEEEPPTTQNEL 147
           LNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+++ +  +G EE+        
Sbjct: 80  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEGAEED-------- 139

Query: 148 AENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAM 207
              E PFEFRALEV LEAICSFL ART ELE+  YPALDELTSKISSRNLDRVRKLKS M
Sbjct: 140 ---ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKSGM 199

Query: 208 TRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESGCGTPIWFLASPRDYSKMS 267
           TRL  RVQKVRDELEQLLDDDDDMA+LYLSRK+AG  +P SG G P WF ASP   SK+S
Sbjct: 200 TRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASPTIGSKIS 259

Query: 268 KTSRASAITIR-EENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHR 327
           + SRASA TI   ENDVEELEMLLEAYFMQI+GTLNKL TLREYIDDTEDYINIQLDNHR
Sbjct: 260 RASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDNHR 319

Query: 328 NQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATLYMS 387
           NQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +VFKWVV+++G+ CA +++S
Sbjct: 320 NQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVLVSGLFCAFMFVS 379

Query: 388 LSSYARYKGLVGS 397
           + +YAR+KGLVGS
Sbjct: 380 IVAYARHKGLVGS 381

BLAST of Lcy02g018460 vs. ExPASy Swiss-Prot
Match: Q10D38 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.9e-144
Identity = 274/373 (73.46%), Postives = 310/373 (83.11%), Query Frame = 0

Query: 28  KNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRERVIV 87
           K    SRSW+  D  G   VLD DKYAIM RV INARDLR+LDPLLSYPSTILGRER IV
Sbjct: 23  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 82

Query: 88  LNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTSSIYQGQGEEEEPPTTQNEL 147
           LNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+++ +  +G EE+        
Sbjct: 83  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEGAEED-------- 142

Query: 148 AENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAM 207
              E PFEFRALEV LEAICSFL ART ELE+  YPALDELTSKISSRNLDRVRKLKS M
Sbjct: 143 ---ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKSGM 202

Query: 208 TRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESGCGTPIWFLASPRDYSKMS 267
           TRL  RVQKVRDELEQLLDDDDDMA+LYLSRK+AG  +P SG G P WF ASP   SK+S
Sbjct: 203 TRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASPTIGSKIS 262

Query: 268 KTSRASAITIR-EENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHR 327
           + SRASA TI   ENDVEELEMLLEAYFMQI+GTLNKL TLREYIDDTEDYINIQLDNHR
Sbjct: 263 RASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDNHR 322

Query: 328 NQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATLYMS 387
           NQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +VFKWVV+++G+ CA +++S
Sbjct: 323 NQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVLVSGLFCAFMFVS 382

Query: 388 LSSYARYKGLVGS 397
           + +YAR+KGLVGS
Sbjct: 383 IVAYARHKGLVGS 384

BLAST of Lcy02g018460 vs. ExPASy Swiss-Prot
Match: Q9FLG2 (Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 4.6e-142
Identity = 279/403 (69.23%), Postives = 319/403 (79.16%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           MA +G+++P +  AV   KKKT            SW  +D  G+S  LDVDKY IM RVQ
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTP---------QASWALIDATGQSEPLDVDKYEIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+
Sbjct: 61  IHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELR 120

Query: 121 RRLP-STSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTREL 180
           RRLP   +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART EL
Sbjct: 121 RRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAEL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA+LYLS
Sbjct: 181 ETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLS 240

Query: 241 RKV--AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIR-EENDVEELEMLLEAYFMQ 300
           RK+  A +P S  G P W+  SP   SK+S+ SRAS  T+  +ENDVEELEMLLEAYFMQ
Sbjct: 241 RKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQ 300

Query: 301 IEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMN 360
           I+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMN
Sbjct: 301 IDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMN 360

Query: 361 IPYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           IPYTW + H ++FK+VV LTG +C  +++ + SYARYKGLVGS
Sbjct: 361 IPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Lcy02g018460 vs. ExPASy Swiss-Prot
Match: Q304A0 (Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.4e-135
Identity = 262/402 (65.17%), Postives = 309/402 (76.87%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           M+ DG ++PV+S AV        T K+K + +SRSW+S+D  G+ TVLDVDK+ IM RVQ
Sbjct: 1   MSPDGELVPVDSSAV-------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE Q
Sbjct: 61  IHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQ 120

Query: 121 RRLPSTSSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELET 180
           RRLP  +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE 
Sbjct: 121 RRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEK 180

Query: 181 DTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRK 240
             YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LYL+RK
Sbjct: 181 FAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRK 240

Query: 241 VAGTPE--SGCGTPIWFLASPRDYSKMSKTSRASAITIR--EENDVEELEMLLEAYFMQI 300
           + G     S    PIW+  SP   S +S+ SR S +T+R  +E DVEELEMLLEAYFMQI
Sbjct: 241 LVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQI 300

Query: 301 EGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNI 360
           + TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IFGMNI
Sbjct: 301 DSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNI 360

Query: 361 PYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           P TW  DH ++FKWVV LTG  C  L++ + SYAR++GL+GS
Sbjct: 361 PNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Lcy02g018460 vs. ExPASy Swiss-Prot
Match: P0CZ21 (Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 6.0e-110
Identity = 219/370 (59.19%), Postives = 280/370 (75.68%), Query Frame = 0

Query: 33  SRSWVSLDHEGRSTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEH 92
           S SW+S+D  G+ TVLDVDKY IM RVQI+ARDLR+LDP L YPS ILGRER IVLNLEH
Sbjct: 18  SWSWISIDATGKKTVLDVDKYVIMHRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEH 77

Query: 93  IKSIITADEVLLRDPMDENVVPIVEELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFP 152
           IK+IITA EVL++D  DEN++P +EE Q RL   +  + GQ + +        + E+E  
Sbjct: 78  IKAIITAKEVLIQDSSDENLIPTLEEFQTRLSVGNKAHGGQLDGD-------VVEEDESA 137

Query: 153 FEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNR 212
           FEFRALEVALEAICSFL ART ELE   YPALDELT K++SRNL RV KLKS+MTRLT +
Sbjct: 138 FEFRALEVALEAICSFLAARTIELEKSAYPALDELTLKLTSRNLLRVCKLKSSMTRLTAQ 197

Query: 213 VQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESGCGTPI-WFLASPRDYSKMSKTS-- 272
           VQK++DELEQLL+DD+DMAELYLSRK+AG  +P    G  I W+  SP   +K+S+    
Sbjct: 198 VQKIKDELEQLLEDDEDMAELYLSRKLAGASSPAIDSGEHINWYPTSPTIGAKISRAKSH 257

Query: 273 --RASAITIREENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQ 332
             R++ +   ++NDVEE+EMLLEA+FMQI+ TLNKL  LREY+D+TED++NIQLD+ RNQ
Sbjct: 258 LVRSATVRGDDKNDVEEVEMLLEAHFMQIDRTLNKLTELREYVDETEDFLNIQLDSSRNQ 317

Query: 333 LIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATLYMSLS 392
           LI+ E+ L++G++C+S+YS+V  I GMNIP+ W    +H+FKWVV  T  VCA L++++ 
Sbjct: 318 LIKFEIILTAGSICVSVYSVVVGILGMNIPFPW-NIKKHMFKWVVSGTATVCAILFVTIM 377

Query: 393 SYARYKGLVG 396
           S+ARYK L G
Sbjct: 378 SFARYKKLFG 379

BLAST of Lcy02g018460 vs. ExPASy TrEMBL
Match: A0A6J1HGA4 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.3e-204
Identity = 379/400 (94.75%), Postives = 384/400 (96.00%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK----KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGDGF  PVESQAVSVKKK     TTT KKKNT VSRSWVSLDHEGRSTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP  TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           TWKEDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Lcy02g018460 vs. ExPASy TrEMBL
Match: A0A6J1HR33 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 5.7e-204
Identity = 377/397 (94.96%), Postives = 383/397 (96.47%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK-KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           MAGDGF  PVESQ VSVKKK  TTT KKKNT VSRSWVSLDHEGR+TVLDVDKYAIMQRV
Sbjct: 1   MAGDGFGFPVESQVVSVKKKTATTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRV 60

Query: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240
            YPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV
Sbjct: 181 AYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240

Query: 241 AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTLN 300
           AGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIEGTLN
Sbjct: 241 AGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEGTLN 300

Query: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360
           KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK
Sbjct: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360

Query: 361 EDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           EDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 EDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 397

BLAST of Lcy02g018460 vs. ExPASy TrEMBL
Match: A0A0A0LLQ5 (Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.7e-203
Identity = 374/397 (94.21%), Postives = 384/397 (96.73%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSV-KKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GDGFVIPVESQ  SV KKKK TT KKK TAVSRSWVSLD EGRST+LDVDKY IM+RV
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQTTTKKKTTAVSRSWVSLDREGRSTILDVDKYVIMERV 60

Query: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240

Query: 241 AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTLN 300
           AGTPESG GTPIWFLASP+DYSK+S+TSR SAITIR ENDVEELEMLLEAYFMQIEGTLN
Sbjct: 241 AGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIEGTLN 300

Query: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360
           KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK
Sbjct: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360

Query: 361 EDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           EDHEHVFKWVVI+TGIVCAT+Y+SLSSYARYKGLVGS
Sbjct: 361 EDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGS 397

BLAST of Lcy02g018460 vs. ExPASy TrEMBL
Match: A0A1S3BLU7 (Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491255 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.2e-201
Identity = 370/397 (93.20%), Postives = 383/397 (96.47%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSV-KKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GDGFVIPVESQ  SV KKKK +T KKK TAVSRSWVSLD +GRST+LDVDKYAIM+R 
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIMERA 60

Query: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDP DENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240

Query: 241 AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTLN 300
           AGTPESG GTPIWFLASP+DYSK+S+TSR SAITIR ENDVEELEMLLEAYFMQIEGTLN
Sbjct: 241 AGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIEGTLN 300

Query: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360
           KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK
Sbjct: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360

Query: 361 EDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           EDHEHVFKWVVI+TGIVCAT+Y+SLSSYARYKGLVG+
Sbjct: 361 EDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGT 397

BLAST of Lcy02g018460 vs. ExPASy TrEMBL
Match: A0A5D3E2W6 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001010 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.9e-200
Identity = 370/398 (92.96%), Postives = 383/398 (96.23%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSV-KKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           M GDGFVIPVESQ  SV KKKK +T KKK TAVSRSWVSLD +GRST+LDVDKYAIM+R 
Sbjct: 1   MGGDGFVIPVESQVSSVKKKKKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIMERA 60

Query: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDP DENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYLSRK 240
           TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYLSRK
Sbjct: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYLSRK 240

Query: 241 VAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTL 300
           VAGTPESG GTPIWFLASP+DYSK+S+TSR SAITIR ENDVEELEMLLEAYFMQIEGTL
Sbjct: 241 VAGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIEGTL 300

Query: 301 NKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTW 360
           NKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTW
Sbjct: 301 NKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTW 360

Query: 361 KEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           KEDHEHVFKWVVI+TGIVCAT+Y+SLSSYARYKGLVG+
Sbjct: 361 KEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGT 398

BLAST of Lcy02g018460 vs. NCBI nr
Match: KAG6571485.1 (Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011247.1 Magnesium transporter MRS2-7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 722.2 bits (1863), Expect = 2.4e-204
Identity = 379/400 (94.75%), Postives = 385/400 (96.25%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK----KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGDGF  PVESQAVSVKKK     TTT KKKNT VSRSWVSLDHEGR+TVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           TWKEDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Lcy02g018460 vs. NCBI nr
Match: XP_038888713.1 (magnesium transporter MRS2-I-like [Benincasa hispida])

HSP 1 Score: 721.5 bits (1861), Expect = 4.0e-204
Identity = 378/399 (94.74%), Postives = 386/399 (96.74%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKK---TTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQ 60
           MAG+GFVIPVESQ  SVKKKK   TTTKKKKNTA+SRSWVSLD EGRST+LDVDKYAIMQ
Sbjct: 1   MAGNGFVIPVESQVGSVKKKKKKQTTTKKKKNTAISRSWVSLDREGRSTILDVDKYAIMQ 60

Query: 61  RVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVE 120
           RVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVE
Sbjct: 61  RVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVE 120

Query: 121 ELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELE 180
           ELQRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICS LDARTRELE
Sbjct: 121 ELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSCLDARTRELE 180

Query: 181 TDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSR 240
           TDTYPALDELTSKISS NLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSR
Sbjct: 181 TDTYPALDELTSKISSLNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSR 240

Query: 241 KVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGT 300
           KVAGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIE T
Sbjct: 241 KVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITTRGENDVEELEMLLEAYFMQIEST 300

Query: 301 LNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYT 360
           LNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYT
Sbjct: 301 LNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYT 360

Query: 361 WKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           WKEDHEHVFKWVVILTGIVCAT+Y+SLSSYARYKGLVGS
Sbjct: 361 WKEDHEHVFKWVVILTGIVCATIYVSLSSYARYKGLVGS 399

BLAST of Lcy02g018460 vs. NCBI nr
Match: XP_023554091.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.1 bits (1860), Expect = 5.3e-204
Identity = 378/401 (94.26%), Postives = 385/401 (96.01%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK-----KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAI 60
           MAGDGF  PVESQAVSVKKK      TTT KKKNT VSRSWVSLDHEGR+TVLDVDKYA+
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTATTTTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAL 60

Query: 61  MQRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           MQRVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPI
Sbjct: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120

Query: 121 VEELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180
           VEELQRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRE
Sbjct: 121 VEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180

Query: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL 240
           LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL
Sbjct: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIE 300
           SRKVAGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIE
Sbjct: 241 SRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIE 300

Query: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360
           GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP
Sbjct: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360

Query: 361 YTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           YTWKEDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 YTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401

BLAST of Lcy02g018460 vs. NCBI nr
Match: XP_022963506.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 720.7 bits (1859), Expect = 6.9e-204
Identity = 379/400 (94.75%), Postives = 384/400 (96.00%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK----KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIM 60
           MAGDGF  PVESQAVSVKKK     TTT KKKNT VSRSWVSLDHEGRSTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPST+S+YQGQGEEEEP  TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           TWKEDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Lcy02g018460 vs. NCBI nr
Match: XP_022967532.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 719.9 bits (1857), Expect = 1.2e-203
Identity = 377/397 (94.96%), Postives = 383/397 (96.47%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKK-KTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRV 60
           MAGDGF  PVESQ VSVKKK  TTT KKKNT VSRSWVSLDHEGR+TVLDVDKYAIMQRV
Sbjct: 1   MAGDGFGFPVESQVVSVKKKTATTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRV 60

Query: 61  QINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120
           QINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL
Sbjct: 61  QINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEEL 120

Query: 121 QRRLPSTSSIYQGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180
           QRRLPST+S+YQGQGEEEEP TTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD
Sbjct: 121 QRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETD 180

Query: 181 TYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240
            YPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV
Sbjct: 181 AYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKV 240

Query: 241 AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIREENDVEELEMLLEAYFMQIEGTLN 300
           AGTPESG GTPIWFLASP+DYSKMSKTSRASAIT R ENDVEELEMLLEAYFMQIEGTLN
Sbjct: 241 AGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEGTLN 300

Query: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360
           KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK
Sbjct: 301 KLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWK 360

Query: 361 EDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           EDHEHVFKWVVILTGIVCAT+Y+SLSSYAR KGLVGS
Sbjct: 361 EDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 397

BLAST of Lcy02g018460 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 505.8 bits (1301), Expect = 3.2e-143
Identity = 279/403 (69.23%), Postives = 319/403 (79.16%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           MA +G+++P +  AV   KKKT            SW  +D  G+S  LDVDKY IM RVQ
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTP---------QASWALIDATGQSEPLDVDKYEIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+
Sbjct: 61  IHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELR 120

Query: 121 RRLP-STSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTREL 180
           RRLP   +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART EL
Sbjct: 121 RRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAEL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA+LYLS
Sbjct: 181 ETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLS 240

Query: 241 RKV--AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIR-EENDVEELEMLLEAYFMQ 300
           RK+  A +P S  G P W+  SP   SK+S+ SRAS  T+  +ENDVEELEMLLEAYFMQ
Sbjct: 241 RKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQ 300

Query: 301 IEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMN 360
           I+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMN
Sbjct: 301 IDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMN 360

Query: 361 IPYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           IPYTW + H ++FK+VV LTG +C  +++ + SYARYKGLVGS
Sbjct: 361 IPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Lcy02g018460 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 483.4 bits (1243), Expect = 1.7e-136
Identity = 262/402 (65.17%), Postives = 309/402 (76.87%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           M+ DG ++PV+S AV        T K+K + +SRSW+S+D  G+ TVLDVDK+ IM RVQ
Sbjct: 1   MSPDGELVPVDSSAV-------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE Q
Sbjct: 61  IHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQ 120

Query: 121 RRLPSTSSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELET 180
           RRLP  +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE 
Sbjct: 121 RRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEK 180

Query: 181 DTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRK 240
             YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LYL+RK
Sbjct: 181 FAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRK 240

Query: 241 VAGTPE--SGCGTPIWFLASPRDYSKMSKTSRASAITIR--EENDVEELEMLLEAYFMQI 300
           + G     S    PIW+  SP   S +S+ SR S +T+R  +E DVEELEMLLEAYFMQI
Sbjct: 241 LVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQI 300

Query: 301 EGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNI 360
           + TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IFGMNI
Sbjct: 301 DSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNI 360

Query: 361 PYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           P TW  DH ++FKWVV LTG  C  L++ + SYAR++GL+GS
Sbjct: 361 PNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Lcy02g018460 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 483.4 bits (1243), Expect = 1.7e-136
Identity = 262/402 (65.17%), Postives = 309/402 (76.87%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           M+ DG ++PV+S AV        T K+K + +SRSW+S+D  G+ TVLDVDK+ IM RVQ
Sbjct: 12  MSPDGELVPVDSSAV-------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQ 71

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE Q
Sbjct: 72  IHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQ 131

Query: 121 RRLPSTSSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELET 180
           RRLP  +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE 
Sbjct: 132 RRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEK 191

Query: 181 DTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRK 240
             YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LYL+RK
Sbjct: 192 FAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRK 251

Query: 241 VAGTPE--SGCGTPIWFLASPRDYSKMSKTSRASAITIR--EENDVEELEMLLEAYFMQI 300
           + G     S    PIW+  SP   S +S+ SR S +T+R  +E DVEELEMLLEAYFMQI
Sbjct: 252 LVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQI 311

Query: 301 EGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNI 360
           + TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IFGMNI
Sbjct: 312 DSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNI 371

Query: 361 PYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           P TW  DH ++FKWVV LTG  C  L++ + SYAR++GL+GS
Sbjct: 372 PNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 397

BLAST of Lcy02g018460 vs. TAIR 10
Match: AT5G64560.2 (magnesium transporter 9 )

HSP 1 Score: 473.4 bits (1217), Expect = 1.8e-133
Identity = 263/376 (69.95%), Postives = 298/376 (79.26%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           MA +G+++P +  AV   KKKT            SW  +D  G+S  LDVDKY IM RVQ
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTP---------QASWALIDATGQSEPLDVDKYEIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP LSYPSTILGRER IVLNLEHIK+IIT++EVLLRDP DENV+P+VEEL+
Sbjct: 61  IHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELR 120

Query: 121 RRLP-STSSIYQGQGEEEEPPTTQNE---LAENEFPFEFRALEVALEAICSFLDARTREL 180
           RRLP   +S   GQG+ +E    QN+     E+E PFEFRALEVALEAICSFL ART EL
Sbjct: 121 RRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAEL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA+LYLS
Sbjct: 181 ETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLS 240

Query: 241 RKV--AGTPESGCGTPIWFLASPRDYSKMSKTSRASAITIR-EENDVEELEMLLEAYFMQ 300
           RK+  A +P S  G P W+  SP   SK+S+ SRAS  T+  +ENDVEELEMLLEAYFMQ
Sbjct: 241 RKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQ 300

Query: 301 IEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMN 360
           I+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMN
Sbjct: 301 IDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMN 360

Query: 361 IPYTWKEDHEHVFKWV 370
           IPYTW + H ++FK+V
Sbjct: 361 IPYTWNDGHGYMFKYV 367

BLAST of Lcy02g018460 vs. TAIR 10
Match: AT5G09690.4 (magnesium transporter 7 )

HSP 1 Score: 452.2 bits (1162), Expect = 4.3e-127
Identity = 251/402 (62.44%), Postives = 297/402 (73.88%), Query Frame = 0

Query: 1   MAGDGFVIPVESQAVSVKKKKTTTKKKKNTAVSRSWVSLDHEGRSTVLDVDKYAIMQRVQ 60
           M+ DG ++PV+S AV        T K+K + +SRSW+S+D  G+ TVLDVDK+ IM RVQ
Sbjct: 1   MSPDGELVPVDSSAV-------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQ 60

Query: 61  INARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQ 120
           I+ARDLR+LDP L YPS ILGRER IVLNLEHIK+IITA+EVL+RD  DENV+P++EE Q
Sbjct: 61  IHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQ 120

Query: 121 RRLPSTSSIY--QGQGEEEEPPTTQNELAENEFPFEFRALEVALEAICSFLDARTRELET 180
           RRLP  +  +   G G+  E         E+E PFEFRALEVALEAICSFL ART ELE 
Sbjct: 121 RRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTTELEK 180

Query: 181 DTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRK 240
             YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LYL+RK
Sbjct: 181 FAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRK 240

Query: 241 VAGTPE--SGCGTPIWFLASPRDYSKMSKTSRASAITIR--EENDVEELEMLLEAYFMQI 300
           + G     S    PIW+  SP   S +S+ SR S +T+R  +E DVEELEMLLE      
Sbjct: 241 LVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLE------ 300

Query: 301 EGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNI 360
                    LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IFGMNI
Sbjct: 301 ---------LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNI 360

Query: 361 PYTWKEDHEHVFKWVVILTGIVCATLYMSLSSYARYKGLVGS 397
           P TW  DH ++FKWVV LTG  C  L++ + SYAR++GL+GS
Sbjct: 361 PNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B8AJT92.9e-14473.46Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE... [more]
Q10D382.9e-14473.46Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I ... [more]
Q9FLG24.6e-14269.23Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1[more]
Q304A02.4e-13565.17Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1[more]
P0CZ216.0e-11059.19Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HGA43.3e-20494.75Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1[more]
A0A6J1HR335.7e-20494.96Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1[more]
A0A0A0LLQ51.7e-20394.21Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1[more]
A0A1S3BLU71.2e-20193.20Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491255 PE=3 SV=1[more]
A0A5D3E2W62.9e-20092.96Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
Match NameE-valueIdentityDescription
KAG6571485.12.4e-20494.75Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_038888713.14.0e-20494.74magnesium transporter MRS2-I-like [Benincasa hispida][more]
XP_023554091.15.3e-20494.26magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022963506.16.9e-20494.75magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata][more]
XP_022967532.11.2e-20394.96magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G64560.13.2e-14369.23magnesium transporter 9 [more]
AT5G09690.11.7e-13665.17magnesium transporter 7 [more]
AT5G09690.21.7e-13665.17magnesium transporter 7 [more]
AT5G64560.21.8e-13369.95magnesium transporter 9 [more]
AT5G09690.44.3e-12762.44magnesium transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..226
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 110..326
e-value: 5.0E-68
score: 229.9
NoneNo IPR availableGENE3D2.40.128.330coord: 18..107
e-value: 3.3E-20
score: 73.3
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 333..387
e-value: 4.6E-5
score: 25.5
NoneNo IPR availablePANTHERPTHR13890:SF46MAGNESIUM TRANSPORTER MRS2-LIKE PROTEINcoord: 6..396
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 332..383
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 283..382
e-value: 5.4E-7
score: 29.3
IPR039204Magnesium transporter MRS2-likePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 6..396
IPR039204Magnesium transporter MRS2-likeCDDcd12823Mrs2_Mfm1p-likecoord: 36..393
e-value: 1.21438E-101
score: 302.241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g018460.1Lcy02g018460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity