Lcy02g017140 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g017140
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr02: 44335539 .. 44338503 (+)
RNA-Seq ExpressionLcy02g017140
SyntenyLcy02g017140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAGTGTATATGTGGTAGCTGTAGAATTTCCTCAGTTTTTAAGTTCATAGAATTCTTGAGAATGGAAGCACAGGAAAATTGGTGAGCTCTCCATGCCTTTAATGGCGGATTAAGAGAGAGAAACTCCAAATTCAAGTAATGGGGAGTTGAAAATTTTTTCAGAATGGAAACTTTCCTTTTCAATCGTCGTCGTCTGTAGCAGCGTTCGTCAAGTCTAGTCTCTTCTCTTCTCTTCCCGTTGAAGTGATCCAAACCTGCTTCCGATTTTGATGGCGAAGACGGGCGTGTTTGATTCCAATCCTACAGTCATGGGGAAGGCGACTGAGCTGAAGAGGGAGCTACAGAGACTCATCGTGGCTATAATTGATGACGATAATTACTCCACCGACACCATCGATCATGCTAAAGAGATTCTCTGTGCGCTCAAGGAGTTGAAACTCAGGAAGAGATCGCGCTCTTCGAATCTCTACGAGAATCTCATTTGCCCTGATGAATTCCGCTGTCCGCTTTCTCGAGAGCTCATGAGAGATCCTGTTGTTGTCTCTTCTGGCGAGGTTTGTTTTCATGGCTGTGTTCTGTGATTTCTCTTCTAGGTTATGGTGATTTTGATTGTGATGATTGAAATTTTCTTCTCGTTTCTCCGTCTTTGTGATGAGAACGCTTTGATATGTTTGCGGTTTTGTTGGTTTCATTTCGGGGGGTTTTCTTTCCCCATTCGCGGTTGAATCCAGTGTTTGTGGATCAACATACTAAGCGCACAGCGAAACGAAAAATTTGAGGACATCAGTGTGTTCTTCATCTTACTTTTCGTTCTTCTCTGCTTTCGTTCAATGTTGTTCTGTAAATCTGCTGTTTGGATTTGTCTTCTTCTTCTTCTTCGTTTTCTTCAAATTACATCTCCTCTGTTTAGTGAACGGAGAAGTGATTTCAAGAATAAAATATTTTCCAATTCGATATTTACAATCTGTAGGATTTTCAGGTTTCTGTGCTTTTAGTTTAGAATTTTCAGTTTAACTTCTGTTTTGTGACTGTATTGTCACTGACATTGCATCTTTTGTATTTAAAATTTTCGAGAGCCGACGATTTGAACCCTTTTTCAGTTACTGTATTCATGTTTCGTATTGTATTTGAAGGTAACAGATATTGATTGTGAAGACATATGAGTGATTTTCCAGAAAATCTGTATAATTTGTTATATGTATGATTTTGATGGACTAGATCTAATACCATTGTGAATCCTTTGTTACCTCAAGACATTCATTATGGTCATTTTTGAGTTTTCTATGTGTTATTGTTTTGTTCATCTCATTGCAGACGTACGACAGACCCTTCATAAATAAATGGTTAAAATCAGGAAACCGAACGTGCCCCCGAACTCAGCAAGTTCTTTCGCACACGAACCTCACTCCGAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTTCAATATCTTGATGACGATGTAATAACGGAGGCTGATAGAGACCGTTTTCTTACGTTGATAGAGAAAATGTCTACGACATTATCTGAACAGAAAGAAGCTGCTAAGGAGCTTCGGATGTTGACGAAGCGGATGCCCTCATTTCGAGCACTTTTTGGCGAGTCCCTTGATGCCATCTCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTAGTATTCATGCGGATCTGCAAGAAGATGTGATCACGACCCTCTTGAATCTGTCAATCCATGACAACAACAAGAAGCTTGTTGCTGAAACTCCCAAGGTAGTACCTCTTCTAATGGAAGCGTTGAGATCGGGAACCATGGAAACAAGGAGCAATGCAGCTGCAGCCCTTTTTACTTTGTCAGCTCTCGACTCAAACAAAGCACTCATCGGGAAAGCGGGTGCCTTGAAACCACTTATCGACCTTTTAGACGAGGGACATCCATTATCTATCAAAGATGCTGCTTCAGCAATATTTAATCTTTGTATTATTCATGAAAATAAAGGAAGAGCTGTTCGAGATGGTGCAGTTCGAGTGCTTCTGAAAAAGATCATGAACCAAATGCACGTCGACGAGTTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCGAAAGCTATCGAGGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGGGAAAATGCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCACGCTATCTGCTTATACGATCGAACCAAGTGGAAGGACATGAGGGAGGAGGAGAAATGCTATCGAACTATCTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTCGAAAGACTGAACAGGGCAGTTAATATGACCCATACCGCATAATGAAACTCTCAGCGTTAAAAATTTGTAGATTCTGCCTCTAAATCTGGCACTCTGGTCTTTGCTAAGACTCGGTCATCTCATCTGTTTGGGTAATCTTCAATCATCTTTTTTGTATATTCTCTTTTCCTGTGGAGTCTAATTTCGATTTCACTGCCAGGTTCATCAGTTAGTCACTGTGAATAAATGTGAAGTGAGTTCTGTTGATAAGTTTTTACTTTTGTTTCTGTTTTTAGCTTTACTTGTTCTTTTGACATTGGTTCTTTTCATCATTACTGCACTTTGCGAATGCTAGCTAGTTTCTCTCCCTTTTCTCTGTTTCATTGAACTGAACTGATAAGTTTAGAGCTGTTCAAAAGGAAGTGAGCAAATATGATTGGAGAGAATCTTAGAAGAGAGGATATGATTGTTTTCTTGCATATGTTCCTAAGTGGTACCAAATGGATCTAACATTGGACAAAGAAAGAGCCAGTGAATGGTAACAGGGAAAGTAATGTGCATCATTTTGAATAGTGTCTGTGATATCTTGTAATGTGGTCCATGGAAGATGGATGGAATAGGAATA

mRNA sequence

GAGAAAGTGTATATGTGGTAGCTGTAGAATTTCCTCAGTTTTTAAGTTCATAGAATTCTTGAGAATGGAAGCACAGGAAAATTGGTGAGCTCTCCATGCCTTTAATGGCGGATTAAGAGAGAGAAACTCCAAATTCAAGTAATGGGGAGTTGAAAATTTTTTCAGAATGGAAACTTTCCTTTTCAATCGTCGTCGTCTGTAGCAGCGTTCGTCAAGTCTAGTCTCTTCTCTTCTCTTCCCGTTGAAGTGATCCAAACCTGCTTCCGATTTTGATGGCGAAGACGGGCGTGTTTGATTCCAATCCTACAGTCATGGGGAAGGCGACTGAGCTGAAGAGGGAGCTACAGAGACTCATCGTGGCTATAATTGATGACGATAATTACTCCACCGACACCATCGATCATGCTAAAGAGATTCTCTGTGCGCTCAAGGAGTTGAAACTCAGGAAGAGATCGCGCTCTTCGAATCTCTACGAGAATCTCATTTGCCCTGATGAATTCCGCTGTCCGCTTTCTCGAGAGCTCATGAGAGATCCTGTTGTTGTCTCTTCTGGCGAGACGTACGACAGACCCTTCATAAATAAATGGTTAAAATCAGGAAACCGAACGTGCCCCCGAACTCAGCAAGTTCTTTCGCACACGAACCTCACTCCGAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTTCAATATCTTGATGACGATGTAATAACGGAGGCTGATAGAGACCGTTTTCTTACGTTGATAGAGAAAATGTCTACGACATTATCTGAACAGAAAGAAGCTGCTAAGGAGCTTCGGATGTTGACGAAGCGGATGCCCTCATTTCGAGCACTTTTTGGCGAGTCCCTTGATGCCATCTCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTAGTATTCATGCGGATCTGCAAGAAGATGTGATCACGACCCTCTTGAATCTGTCAATCCATGACAACAACAAGAAGCTTGTTGCTGAAACTCCCAAGGTAGTACCTCTTCTAATGGAAGCGTTGAGATCGGGAACCATGGAAACAAGGAGCAATGCAGCTGCAGCCCTTTTTACTTTGTCAGCTCTCGACTCAAACAAAGCACTCATCGGGAAAGCGGGTGCCTTGAAACCACTTATCGACCTTTTAGACGAGGGACATCCATTATCTATCAAAGATGCTGCTTCAGCAATATTTAATCTTTGTATTATTCATGAAAATAAAGGAAGAGCTGTTCGAGATGGTGCAGTTCGAGTGCTTCTGAAAAAGATCATGAACCAAATGCACGTCGACGAGTTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCGAAAGCTATCGAGGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGGGAAAATGCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCACGCTATCTGCTTATACGATCGAACCAAGTGGAAGGACATGAGGGAGGAGGAGAAATGCTATCGAACTATCTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTCGAAAGACTGAACAGGGCAGTTAATATGACCCATACCGCATAATGAAACTCTCAGCGTTAAAAATTTGTAGATTCTGCCTCTAAATCTGGCACTCTGGTCTTTGCTAAGACTCGGTCATCTCATCTGTTTGGGTAATCTTCAATCATCTTTTTTGTATATTCTCTTTTCCTGTGGAGTCTAATTTCGATTTCACTGCCAGGTTCATCAGTTAGTCACTGTGAATAAATGTGAAGTGAGTTCTGTTGATAAGTTTTTACTTTTGTTTCTGTTTTTAGCTTTACTTGTTCTTTTGACATTGGTTCTTTTCATCATTACTGCACTTTGCGAATGCTAGCTAGTTTCTCTCCCTTTTCTCTGTTTCATTGAACTGAACTGATAAGTTTAGAGCTGTTCAAAAGGAAGTGAGCAAATATGATTGGAGAGAATCTTAGAAGAGAGGATATGATTGTTTTCTTGCATATGTTCCTAAGTGGTACCAAATGGATCTAACATTGGACAAAGAAAGAGCCAGTGAATGGTAACAGGGAAAGTAATGTGCATCATTTTGAATAGTGTCTGTGATATCTTGTAATGTGGTCCATGGAAGATGGATGGAATAGGAATA

Coding sequence (CDS)

ATGGCGAAGACGGGCGTGTTTGATTCCAATCCTACAGTCATGGGGAAGGCGACTGAGCTGAAGAGGGAGCTACAGAGACTCATCGTGGCTATAATTGATGACGATAATTACTCCACCGACACCATCGATCATGCTAAAGAGATTCTCTGTGCGCTCAAGGAGTTGAAACTCAGGAAGAGATCGCGCTCTTCGAATCTCTACGAGAATCTCATTTGCCCTGATGAATTCCGCTGTCCGCTTTCTCGAGAGCTCATGAGAGATCCTGTTGTTGTCTCTTCTGGCGAGACGTACGACAGACCCTTCATAAATAAATGGTTAAAATCAGGAAACCGAACGTGCCCCCGAACTCAGCAAGTTCTTTCGCACACGAACCTCACTCCGAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTTCAATATCTTGATGACGATGTAATAACGGAGGCTGATAGAGACCGTTTTCTTACGTTGATAGAGAAAATGTCTACGACATTATCTGAACAGAAAGAAGCTGCTAAGGAGCTTCGGATGTTGACGAAGCGGATGCCCTCATTTCGAGCACTTTTTGGCGAGTCCCTTGATGCCATCTCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTAGTATTCATGCGGATCTGCAAGAAGATGTGATCACGACCCTCTTGAATCTGTCAATCCATGACAACAACAAGAAGCTTGTTGCTGAAACTCCCAAGGTAGTACCTCTTCTAATGGAAGCGTTGAGATCGGGAACCATGGAAACAAGGAGCAATGCAGCTGCAGCCCTTTTTACTTTGTCAGCTCTCGACTCAAACAAAGCACTCATCGGGAAAGCGGGTGCCTTGAAACCACTTATCGACCTTTTAGACGAGGGACATCCATTATCTATCAAAGATGCTGCTTCAGCAATATTTAATCTTTGTATTATTCATGAAAATAAAGGAAGAGCTGTTCGAGATGGTGCAGTTCGAGTGCTTCTGAAAAAGATCATGAACCAAATGCACGTCGACGAGTTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCGAAAGCTATCGAGGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGGGAAAATGCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCACGCTATCTGCTTATACGATCGAACCAAGTGGAAGGACATGAGGGAGGAGGAGAAATGCTATCGAACTATCTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTCGAAAGACTGAACAGGGCAGTTAATATGACCCATACCGCATAA

Protein sequence

MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKRSRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Homology
BLAST of Lcy02g017140 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.1e-155
Identity = 286/463 (61.77%), Postives = 367/463 (79.27%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PT + KA ELKRE+++L++ I D+D+    TID  ++ L AL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSNL--YENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQ 120
           ++SS+L   E + CP+EFRCPLS ELMRDPVV++SG+TYD+ FI KWL SGNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCTSRGIELQDRVQ---YLDDDVITEADRDRFLTLIEKMST 180
           VL HT LTPN LIREMIS+WC   G+E + +       +D+ +T +DR+ F +L+ K+S+
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-LSEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVIT 240
           + L +QK AAKELR+LT++   FRALFGES D I+ L++PL      S+    LQEDV+T
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLL---HGSNPDEKLQEDVVT 240

Query: 241 TLLNLSIH-DNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGK 300
           TLLN+SIH D+NKKLV E P V+PLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGK
Sbjct: 241 TLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGK 300

Query: 301 AGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDE 360
           +G LKPLIDLL+EG+PL+IKD A+AIF LCI HEN+ RAVRDGAVRVL KKI N ++VDE
Sbjct: 301 SGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDE 360

Query: 361 LLAILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKD 420
           LLAILAML  H KA+EE+GELG V +LL+I RE+ C RNKEN IVI+H IC  DRTKWK+
Sbjct: 361 LLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKE 420

Query: 421 MREEEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           ++EEE  + TI++L++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 421 IKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lcy02g017140 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.8e-81
Identity = 183/450 (40.67%), Postives = 279/450 (62.00%), Query Frame = 0

Query: 17  ATELKRELQRLIVAIIDD----DNYSTD-------TIDHAKEILCALKELKLRKRSRSSN 76
           A  L+RELQ+++  I++D    D   T+        ID A  IL  L++++ +      +
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPESDIS 68

Query: 77  LYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVLSHTNL 136
             E    P EF C LS  +M +PV+++SG+TY++ +I +WLK   RTCP+T+QVLSH   
Sbjct: 69  PVE---VPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKH-ERTCPKTKQVLSHRLW 128

Query: 137 TPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKM--STTLSEQKEA 196
            PNHLI ++I+QWC     + Q       D+++ E        L++++  S+++++Q EA
Sbjct: 129 IPNHLISDLITQWCLVNKYDHQKP----SDELVAELFTSDIEALLQRVSSSSSVADQIEA 188

Query: 197 AKELRMLTKRMPSFRALFGESL-DAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIH 256
           AKELR  TK+ P+ R  F   + D+I+ LLSPL           +LQE+++T L NLSI 
Sbjct: 189 AKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSIL 248

Query: 257 DNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLID 316
           ++NK ++AE   V+PLL ++L+ GT ETR NAAA L +LSA+DSNK +IG + A+K LID
Sbjct: 249 ESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALID 308

Query: 317 LLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAMLS 376
           L++EG  L+ K+A S +FNLCI+ ENKG+ V  G +    KKI    +VDELL++LA++S
Sbjct: 309 LIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIS 368

Query: 377 NHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAIC--LYDRTKWKDMREEEKC 436
            H +A+EEM +LG +  L  I+R+ +     EN +VI+  +     DR++ K + EEE  
Sbjct: 369 THNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQ 428

Query: 437 YRTISELAQNGTSRAKRKASGILERLNRAV 451
           + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 HGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

BLAST of Lcy02g017140 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.5e-75
Identity = 179/445 (40.22%), Postives = 268/445 (60.22%), Query Frame = 0

Query: 19  ELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRK-------RSRSSNL-YENL 78
           ++K E   L   +I         +D    +L  LK+  + +        +RS+++ + + 
Sbjct: 167 DMKNESIALHNMVISTAGEPDGCVDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHRSP 226

Query: 79  ICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVLSHTNLTPNHL 138
           I PDEFRCP+S ELM+DPV+VSSG+TY+R  I KWL SG++TCP+TQQ LSHT+LTPN +
Sbjct: 227 IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFV 286

Query: 139 IREMISQWCTSRGIELQDRVQYLDDDVITEA---DRDRFLTLIEKM-STTLSEQKEAAKE 198
           ++ +ISQWC + GIEL    Q   D    ++   D    ++L+ ++ S    EQ+ AA E
Sbjct: 287 LKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAGE 346

Query: 199 LRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIHDNNK 258
           +R+L KR  + R    E+  AI LL++ L      SS     QE  +T LLNLSIH+NNK
Sbjct: 347 IRLLAKRNVNNRICIAEA-GAIPLLVNLL------SSSDPRTQEHAVTALLNLSIHENNK 406

Query: 259 KLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLIDLLDE 318
             + ++   +P ++E L++G+METR NAAA LF+LS +D NK  IG AGA+ PLI+LL +
Sbjct: 407 ASIVDS-HAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCD 466

Query: 319 GHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQM--HVDELLAILAMLSNH 378
           G P   KDAA+AIFNLCI   NK RAV+ G V  L+  +++     +DE L++L++L+ +
Sbjct: 467 GSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGN 526

Query: 379 PKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTI 438
           P+    +     +P L+ +I+  +  RN+EN   I+  +C  D T+     +       +
Sbjct: 527 PEGKIVIARSEPIPPLVEVIKTGS-PRNRENAAAILWLLCSAD-TEQTLAAKAAGVEDAL 586

Query: 439 SELAQNGTSRAKRKASGILERLNRA 450
            EL++ GT RAKRKAS ILE +++A
Sbjct: 587 KELSETGTDRAKRKASSILELMHQA 601

BLAST of Lcy02g017140 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.3e-71
Identity = 178/475 (37.47%), Postives = 264/475 (55.58%), Query Frame = 0

Query: 6   VFDSNPTVMGKAT---------ELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELK 65
           V D +P ++ + +         ELK+E   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKR--------SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKS 125
             +         SR  + + + + P+ FRCP+S ELM+DPV+VS+G+TY+R  I KWL +
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIELQD-----RVQYLDDDVITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC S GIEL       R   +     ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DRFLTLIEKMST-TLSEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS 245
              L+L+EK++  T  +Q+ AA ELR+L KR    R    E+  AI LL+  L      S
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELL------S 404

Query: 246 SIHADLQEDVITTLLNLSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLS 305
           S     QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+LS
Sbjct: 405 SPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLS 464

Query: 306 ALDSNKALIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVL- 365
            +D NK  IG AGA++ LI LL+EG     KDAA+AIFNLCI   NK RAV+ G V  L 
Sbjct: 465 VIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLT 524

Query: 366 -LKKIMNQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVII 425
            L K      VDE LAILA+LS + +    + E  ++P L+ IIR  +  RN+EN   I+
Sbjct: 525 RLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGS-PRNRENAAAIL 584

Query: 426 HAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
             +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 WYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

BLAST of Lcy02g017140 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.5e-69
Identity = 162/391 (41.43%), Postives = 235/391 (60.10%), Query Frame = 0

Query: 70  LICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVLSHTNLTPNH 129
           LI P+EFRCP+S ELM DPV+VSSG+TY+R  I KWL+ G+ TCP+TQ+ L+   +TPN+
Sbjct: 254 LIPPEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDIMTPNY 313

Query: 130 LIREMISQWCTSRGIE-------LQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSE-QK 189
           ++R +I+QWC S GIE        Q   +        + + ++   L+ K+++   E ++
Sbjct: 314 VLRSLIAQWCESNGIEPPKRPNISQPSSKASSSSSAPDDEHNKIEELLLKLTSQQPEDRR 373

Query: 190 EAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSI 249
            AA E+R+L K+    R     S  AI LL++ L       S  +  QE  +T++LNLSI
Sbjct: 374 SAAGEIRLLAKQNNHNRVAIAAS-GAIPLLVNLL-----TISNDSRTQEHAVTSILNLSI 433

Query: 250 HDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLI 309
              NK  +  +   VP ++  L+ G+ME R NAAA LF+LS +D NK  IG AGA+ PL+
Sbjct: 434 CQENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKVTIGAAGAIPPLV 493

Query: 310 DLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMH--VDELLAILA 369
            LL EG     KDAA+A+FNLCI   NKG+AVR G V VL++ +       VDE L+ILA
Sbjct: 494 TLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESLSILA 553

Query: 370 MLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEK 429
           +LS+HP    E+G   AVP L+  IR  +  RNKEN   ++  +C +++   + + E +K
Sbjct: 554 ILSSHPDGKSEVGAADAVPVLVDFIRSGS-PRNKENSAAVLVHLCSWNQ---QHLIEAQK 613

Query: 430 --CYRTISELAQNGTSRAKRKASGILERLNR 449
                 + E+A+NGT R KRKA+ +L R +R
Sbjct: 614 LGIMDLLIEMAENGTDRGKRKAAQLLNRFSR 634

BLAST of Lcy02g017140 vs. ExPASy TrEMBL
Match: A0A6J1DYS9 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111025869 PE=4 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 429/456 (94.08%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELK+ELQRLI+AIIDDDNYSTDTIDHAKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSNLYENLICPD+FRCPLSRELMRDPVVVS+GETYDRPFI+KWLK+GNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSEQ 180
           SHTNLTPNHLIREMISQWC SRGIELQDRV YLDDD ITEADRD+FLTLIEKMS T+SEQ
Sbjct: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQFLTLIEKMSMTISEQ 180

Query: 181 KEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLS 240
           K AAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSSIH DLQEDVITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPL 300
           IHDNNKKLVAETPKV+PLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGK+GAL+PL
Sbjct: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300

Query: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAM 360
           IDLLDEGHPL+IKDAASAIFNLCIIHENK RAVRDGAVRVLLKKIM QMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKC 420
           LSNHPKAIEEMGELGAVPFLLRIIRE+ CSRNKENCIVIIHAICLYDRTKWKD+REEEK 
Sbjct: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

BLAST of Lcy02g017140 vs. ExPASy TrEMBL
Match: A0A6J1HUJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.2e-235
Identity = 422/458 (92.14%), Postives = 441/458 (96.29%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSN YENLICPDEFRCPLSRELMRDPVVVS+GETYDRPFI+KWL+SGNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQY--LDDDVITEADRDRFLTLIEKMSTTLS 180
           SHTNLTPNHLIRE+ISQWCTSRGIELQDRV+   LDDDVITEADRDRFLTLIE MS T+S
Sbjct: 121 SHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIS 180

Query: 181 EQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240
           EQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSS+HADLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALK 300
           LSIHDNNKKLVAETP V+PLL EALRSGTMETRSNAAAALFTLSALDSNKALIGK+GALK
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAIL 360
           PLIDLLDEGHPLSIKDAASAIFNLCI+HENK RAVRDGAVRVLLKKIMNQMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRE++CSRNKENCIVIIHAICLYDRTKWKDM+EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELA+NGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lcy02g017140 vs. ExPASy TrEMBL
Match: A0A6J1HKK5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.2e-235
Identity = 422/458 (92.14%), Postives = 440/458 (96.07%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSN YENLICPDEFRCPLSRELMRDPVVVS+GETYDRPFI+KWL+SGNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQY--LDDDVITEADRDRFLTLIEKMSTTLS 180
           SHTNLTPNHLIREMISQWCTSRGIELQDRV+   LDDDVITEADRDRFLTLIE MS T+ 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240
           EQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSS+HADLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALK 300
           LSIHDNNKKLVAETP V+PLL EALRSGTMETRSNAAAALFTLSALDSNKALIGK+GALK
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAIL 360
           PLIDLLDEGHPLS+KDAASAIFNLCI+HENK RAVRDGAVRVLLKKIMNQMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRE++CSRNKENCIVIIHAICLYDRTKWKDM+EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lcy02g017140 vs. ExPASy TrEMBL
Match: A0A6J1K749 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491272 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.1e-234
Identity = 418/455 (91.87%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSNPTVMGKA +LKRELQ+LIVAIIDDDNYSTD ID AKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSNPTVMGKAMDLKRELQKLIVAIIDDDNYSTDAIDQAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSNLYENLICPDEFRCPLS ELMRDPVVVS+GET+DRPFI KWLKSGNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDEFRCPLSTELMRDPVVVSTGETFDRPFIQKWLKSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSEQ 180
           SHTNLTPNHLIR MIS+WC+SRGIELQDRV YLDDD+ITEADR RF  LIEKMS TL EQ
Sbjct: 121 SHTNLTPNHLIRGMISRWCSSRGIELQDRVHYLDDDIITEADRGRFHRLIEKMSATLPEQ 180

Query: 181 KEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLS 240
           K AAKELRMLTKRMPSFRALFGES DAISLLLSPLCGDKSQSS+HADLQED+ITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESHDAISLLLSPLCGDKSQSSVHADLQEDLITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPL 300
           IHDNNKKLV+ETP+VVPLLMEALRSGTMETRSNAAAALFTLSALDSNK LIGKAGALKPL
Sbjct: 241 IHDNNKKLVSETPRVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKVLIGKAGALKPL 300

Query: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAM 360
           IDLLDEGHPLSIKDAASAIFNLCIIHENK RAVRDGAVRVLLKKIMNQMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKC 420
           LSNHPK+IEEMGEL AV  LLRIIRE+ CSRNKENCIVIIHAICLYDRT+WKDMREEEKC
Sbjct: 361 LSNHPKSIEEMGELRAVHSLLRIIRESTCSRNKENCIVIIHAICLYDRTQWKDMREEEKC 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
           YRTISELAQNGTSRAKRKASGIL+RLNR VN THT
Sbjct: 421 YRTISELAQNGTSRAKRKASGILDRLNRVVNTTHT 455

BLAST of Lcy02g017140 vs. ExPASy TrEMBL
Match: A0A6J1ESZ5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111437439 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 417/455 (91.65%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSNPTV+GKAT+LKRELQ+LIVAIIDDDN+S D ID AKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSNPTVVGKATDLKRELQKLIVAIIDDDNFSIDAIDQAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSNLYENLICPDEFRCPLS ELM+DPVVVS+GET+DRPFI KWLKSGNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDEFRCPLSTELMKDPVVVSTGETFDRPFIQKWLKSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSEQ 180
           SHTNLTPNHLIR MIS+WC+SRGIELQDRV YLDDD+ITEADR RF TLIEKMS TL EQ
Sbjct: 121 SHTNLTPNHLIRGMISRWCSSRGIELQDRVHYLDDDIITEADRGRFHTLIEKMSATLPEQ 180

Query: 181 KEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLS 240
           K AAKELRMLTKRMPSFRALFGES DAISLLLSPLCGDKSQSS+HADLQED+ITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESHDAISLLLSPLCGDKSQSSVHADLQEDLITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPL 300
           IHDNNKKLV+ETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNK LIGKAGALKPL
Sbjct: 241 IHDNNKKLVSETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKVLIGKAGALKPL 300

Query: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAM 360
           IDLLDEGHPLSIKDAASAIFNLCIIHENK RAV+DGAVRVLLKKIMNQMHVD LLAILAM
Sbjct: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKARAVKDGAVRVLLKKIMNQMHVDALLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKC 420
           LSNHPKAIEEMGEL AV  LLRIIRE+ CSRNKENCIVIIHAICLYDRT+WKDMREEEKC
Sbjct: 361 LSNHPKAIEEMGELRAVHSLLRIIRESTCSRNKENCIVIIHAICLYDRTQWKDMREEEKC 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
           YRTISELAQNGTSRAKRKASGILERLNR VN THT
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRVVNTTHT 455

BLAST of Lcy02g017140 vs. NCBI nr
Match: XP_022159455.1 (U-box domain-containing protein 9 [Momordica charantia])

HSP 1 Score: 845.1 bits (2182), Expect = 2.8e-241
Identity = 429/456 (94.08%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELK+ELQRLI+AIIDDDNYSTDTIDHAKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSNLYENLICPD+FRCPLSRELMRDPVVVS+GETYDRPFI+KWLK+GNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKMSTTLSEQ 180
           SHTNLTPNHLIREMISQWC SRGIELQDRV YLDDD ITEADRD+FLTLIEKMS T+SEQ
Sbjct: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQFLTLIEKMSMTISEQ 180

Query: 181 KEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLS 240
           K AAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSSIH DLQEDVITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPL 300
           IHDNNKKLVAETPKV+PLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGK+GAL+PL
Sbjct: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300

Query: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAM 360
           IDLLDEGHPL+IKDAASAIFNLCIIHENK RAVRDGAVRVLLKKIM QMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKC 420
           LSNHPKAIEEMGELGAVPFLLRIIRE+ CSRNKENCIVIIHAICLYDRTKWKD+REEEK 
Sbjct: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

BLAST of Lcy02g017140 vs. NCBI nr
Match: XP_038888241.1 (U-box domain-containing protein 9 [Benincasa hispida])

HSP 1 Score: 828.2 bits (2138), Expect = 3.5e-236
Identity = 429/460 (93.26%), Postives = 444/460 (96.52%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLIVAIIDDDNYSTDTID+AKEIL ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60

Query: 61  SR-SSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQV 120
           SR SSNL+ENLICP+EFRCPLSRELMRDPV+VS+GETYD+PFI KWLKSGNRTCPRTQQV
Sbjct: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDKPFIQKWLKSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVQ--YLDDDVITEADRDRFLTLIEKMSTTL 180
           LSHTNLTPNHLIREMISQWCTSRGIELQDRV+  YLDDDVITEADRDRFLTLIEKMS T+
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180

Query: 181 SEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQ-SSIHADLQEDVITTL 240
           SEQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQ SSIH DLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSSIHTDLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGA 300
           LNLSIHDNNKKLV ETP V+PLLMEAL SGTMETRSNAAAALFTLSALDSNKA+IGKAGA
Sbjct: 241 LNLSIHDNNKKLVIETPNVIPLLMEALGSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLI LLDEGHPLSIKDAASAIFNLCIIHENK RAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIKLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLS+H KAIEE+GELGAVPFLLRIIRE++CSRNKENCIVIIHAICLYDRTKWKDMRE
Sbjct: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMRE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           EEKCYRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 460

BLAST of Lcy02g017140 vs. NCBI nr
Match: XP_022967253.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 822.8 bits (2124), Expect = 1.5e-234
Identity = 422/458 (92.14%), Postives = 441/458 (96.29%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSN YENLICPDEFRCPLSRELMRDPVVVS+GETYDRPFI+KWL+SGNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQY--LDDDVITEADRDRFLTLIEKMSTTLS 180
           SHTNLTPNHLIRE+ISQWCTSRGIELQDRV+   LDDDVITEADRDRFLTLIE MS T+S
Sbjct: 121 SHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIS 180

Query: 181 EQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240
           EQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSS+HADLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALK 300
           LSIHDNNKKLVAETP V+PLL EALRSGTMETRSNAAAALFTLSALDSNKALIGK+GALK
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAIL 360
           PLIDLLDEGHPLSIKDAASAIFNLCI+HENK RAVRDGAVRVLLKKIMNQMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRE++CSRNKENCIVIIHAICLYDRTKWKDM+EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELA+NGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lcy02g017140 vs. NCBI nr
Match: XP_022963574.1 (U-box domain-containing protein 9-like [Cucurbita moschata])

HSP 1 Score: 822.8 bits (2124), Expect = 1.5e-234
Identity = 422/458 (92.14%), Postives = 440/458 (96.07%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSN YENLICPDEFRCPLSRELMRDPVVVS+GETYDRPFI+KWL+SGNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQY--LDDDVITEADRDRFLTLIEKMSTTLS 180
           SHTNLTPNHLIREMISQWCTSRGIELQDRV+   LDDDVITEADRDRFLTLIE MS T+ 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240
           EQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSS+HADLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALK 300
           LSIHDNNKKLVAETP V+PLL EALRSGTMETRSNAAAALFTLSALDSNKALIGK+GALK
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAIL 360
           PLIDLLDEGHPLS+KDAASAIFNLCI+HENK RAVRDGAVRVLLKKIMNQMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRE++CSRNKENCIVIIHAICLYDRTKWKDM+EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lcy02g017140 vs. NCBI nr
Match: XP_023554208.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821.6 bits (2121), Expect = 3.3e-234
Identity = 422/458 (92.14%), Postives = 440/458 (96.07%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKATELKRELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVL 120
           SRSSN YENLICPDEFRCPLSRELMRDPVVVS+GETYDRPFI+KWL+SGNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVQY--LDDDVITEADRDRFLTLIEKMSTTLS 180
           SHTNLTPNHLIREMISQWCTSRGIELQDRV+   L+DDVITEADRDRFLTLIE MS T+ 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLEDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240
           EQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALK 300
           LSIHDNNKKLVAETP V+PLL EALRSGTMETRSNAAAALFTLSALDSNKALIGK+GALK
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAIL 360
           PLIDLLDEGHPLS+KDAASAIFNLCI+HENK RAVRDGAVRVLLKKIMNQMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRE++CSRNKENCIVIIHAICLYDRTKWKDM+EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lcy02g017140 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 551.2 bits (1419), Expect = 7.8e-157
Identity = 286/463 (61.77%), Postives = 367/463 (79.27%), Query Frame = 0

Query: 1   MAKTGVFDSNPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PT + KA ELKRE+++L++ I D+D+    TID  ++ L AL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSNL--YENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQ 120
           ++SS+L   E + CP+EFRCPLS ELMRDPVV++SG+TYD+ FI KWL SGNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCTSRGIELQDRVQ---YLDDDVITEADRDRFLTLIEKMST 180
           VL HT LTPN LIREMIS+WC   G+E + +       +D+ +T +DR+ F +L+ K+S+
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-LSEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVIT 240
           + L +QK AAKELR+LT++   FRALFGES D I+ L++PL      S+    LQEDV+T
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLL---HGSNPDEKLQEDVVT 240

Query: 241 TLLNLSIH-DNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGK 300
           TLLN+SIH D+NKKLV E P V+PLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGK
Sbjct: 241 TLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGK 300

Query: 301 AGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDE 360
           +G LKPLIDLL+EG+PL+IKD A+AIF LCI HEN+ RAVRDGAVRVL KKI N ++VDE
Sbjct: 301 SGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDE 360

Query: 361 LLAILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKD 420
           LLAILAML  H KA+EE+GELG V +LL+I RE+ C RNKEN IVI+H IC  DRTKWK+
Sbjct: 361 LLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKE 420

Query: 421 MREEEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           ++EEE  + TI++L++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 421 IKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lcy02g017140 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 378.3 bits (970), Expect = 9.0e-105
Identity = 202/328 (61.59%), Postives = 260/328 (79.27%), Query Frame = 0

Query: 134 MISQWCTSRGIELQDRVQ---YLDDDVITEADRDRFLTLIEKMSTT-LSEQKEAAKELRM 193
           MIS+WC   G+E + +       +D+ +T +DR+ F +L+ K+S++ L +QK AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 194 LTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIH-DNNKKL 253
           LT++   FRALFGES D I+ L++PL      S+    LQEDV+TTLLN+SIH D+NKKL
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLL---HGSNPDEKLQEDVVTTLLNISIHDDSNKKL 120

Query: 254 VAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLIDLLDEGH 313
           V E P V+PLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGK+G LKPLIDLL+EG+
Sbjct: 121 VCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGN 180

Query: 314 PLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAMLSNHPKAI 373
           PL+IKD A+AIF LCI HEN+ RAVRDGAVRVL KKI N ++VDELLAILAML  H KA+
Sbjct: 181 PLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAV 240

Query: 374 EEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISELA 433
           EE+GELG V +LL+I RE+ C RNKEN IVI+H IC  DRTKWK+++EEE  + TI++L+
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 434 QNGTSRAKRKASGILERLNRAVNMTHTA 457
           + GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lcy02g017140 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 378.3 bits (970), Expect = 9.0e-105
Identity = 202/328 (61.59%), Postives = 260/328 (79.27%), Query Frame = 0

Query: 134 MISQWCTSRGIELQDRVQ---YLDDDVITEADRDRFLTLIEKMSTT-LSEQKEAAKELRM 193
           MIS+WC   G+E + +       +D+ +T +DR+ F +L+ K+S++ L +QK AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 194 LTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIH-DNNKKL 253
           LT++   FRALFGES D I+ L++PL      S+    LQEDV+TTLLN+SIH D+NKKL
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLL---HGSNPDEKLQEDVVTTLLNISIHDDSNKKL 120

Query: 254 VAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLIDLLDEGH 313
           V E P V+PLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGK+G LKPLIDLL+EG+
Sbjct: 121 VCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGN 180

Query: 314 PLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAMLSNHPKAI 373
           PL+IKD A+AIF LCI HEN+ RAVRDGAVRVL KKI N ++VDELLAILAML  H KA+
Sbjct: 181 PLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAV 240

Query: 374 EEMGELGAVPFLLRIIRENACSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISELA 433
           EE+GELG V +LL+I RE+ C RNKEN IVI+H IC  DRTKWK+++EEE  + TI++L+
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 434 QNGTSRAKRKASGILERLNRAVNMTHTA 457
           + GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lcy02g017140 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 4.8e-82
Identity = 183/450 (40.67%), Postives = 279/450 (62.00%), Query Frame = 0

Query: 17  ATELKRELQRLIVAIIDD----DNYSTD-------TIDHAKEILCALKELKLRKRSRSSN 76
           A  L+RELQ+++  I++D    D   T+        ID A  IL  L++++ +      +
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPESDIS 68

Query: 77  LYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKSGNRTCPRTQQVLSHTNL 136
             E    P EF C LS  +M +PV+++SG+TY++ +I +WLK   RTCP+T+QVLSH   
Sbjct: 69  PVE---VPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKH-ERTCPKTKQVLSHRLW 128

Query: 137 TPNHLIREMISQWCTSRGIELQDRVQYLDDDVITEADRDRFLTLIEKM--STTLSEQKEA 196
            PNHLI ++I+QWC     + Q       D+++ E        L++++  S+++++Q EA
Sbjct: 129 IPNHLISDLITQWCLVNKYDHQKP----SDELVAELFTSDIEALLQRVSSSSSVADQIEA 188

Query: 197 AKELRMLTKRMPSFRALFGESL-DAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIH 256
           AKELR  TK+ P+ R  F   + D+I+ LLSPL           +LQE+++T L NLSI 
Sbjct: 189 AKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSIL 248

Query: 257 DNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKAGALKPLID 316
           ++NK ++AE   V+PLL ++L+ GT ETR NAAA L +LSA+DSNK +IG + A+K LID
Sbjct: 249 ESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALID 308

Query: 317 LLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVLLKKIMNQMHVDELLAILAMLS 376
           L++EG  L+ K+A S +FNLCI+ ENKG+ V  G +    KKI    +VDELL++LA++S
Sbjct: 309 LIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIS 368

Query: 377 NHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVIIHAIC--LYDRTKWKDMREEEKC 436
            H +A+EEM +LG +  L  I+R+ +     EN +VI+  +     DR++ K + EEE  
Sbjct: 369 THNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQ 428

Query: 437 YRTISELAQNGTSRAKRKASGILERLNRAV 451
           + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 HGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

BLAST of Lcy02g017140 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 271.9 bits (694), Expect = 9.1e-73
Identity = 178/475 (37.47%), Postives = 264/475 (55.58%), Query Frame = 0

Query: 6   VFDSNPTVMGKAT---------ELKRELQRLIVAIIDDDNYSTDTIDHAKEILCALKELK 65
           V D +P ++ + +         ELK+E   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKR--------SRSSNLYENLICPDEFRCPLSRELMRDPVVVSSGETYDRPFINKWLKS 125
             +         SR  + + + + P+ FRCP+S ELM+DPV+VS+G+TY+R  I KWL +
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIELQD-----RVQYLDDDVITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC S GIEL       R   +     ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DRFLTLIEKMST-TLSEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS 245
              L+L+EK++  T  +Q+ AA ELR+L KR    R    E+  AI LL+  L      S
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELL------S 404

Query: 246 SIHADLQEDVITTLLNLSIHDNNKKLVAETPKVVPLLMEALRSGTMETRSNAAAALFTLS 305
           S     QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+LS
Sbjct: 405 SPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLS 464

Query: 306 ALDSNKALIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKGRAVRDGAVRVL- 365
            +D NK  IG AGA++ LI LL+EG     KDAA+AIFNLCI   NK RAV+ G V  L 
Sbjct: 465 VIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLT 524

Query: 366 -LKKIMNQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRENACSRNKENCIVII 425
            L K      VDE LAILA+LS + +    + E  ++P L+ IIR  +  RN+EN   I+
Sbjct: 525 RLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGS-PRNRENAAAIL 584

Query: 426 HAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
             +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 WYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRT01.1e-15561.77U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q3E9F76.8e-8140.67Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5VRH92.5e-7540.22U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q8VZ401.3e-7137.47U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q9ZV313.5e-6941.43U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1DYS91.4e-24194.08RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102586... [more]
A0A6J1HUJ67.2e-23592.14RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 P... [more]
A0A6J1HKK57.2e-23592.14RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870... [more]
A0A6J1K7496.1e-23491.87RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491272 P... [more]
A0A6J1ESZ52.3e-23391.65RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111437439... [more]
Match NameE-valueIdentityDescription
XP_022159455.12.8e-24194.08U-box domain-containing protein 9 [Momordica charantia][more]
XP_038888241.13.5e-23693.26U-box domain-containing protein 9 [Benincasa hispida][more]
XP_022967253.11.5e-23492.14U-box domain-containing protein 9-like [Cucurbita maxima][more]
XP_022963574.11.5e-23492.14U-box domain-containing protein 9-like [Cucurbita moschata][more]
XP_023554208.13.3e-23492.14U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G07360.17.8e-15761.77plant U-box 9 [more]
AT3G07360.29.0e-10561.59plant U-box 9 [more]
AT3G07360.39.0e-10561.59plant U-box 9 [more]
AT5G18320.14.8e-8240.67ARM repeat superfamily protein [more]
AT3G54850.19.1e-7337.47plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 443..456
NoneNo IPR availableCOILSCoilCoilcoord: 170..190
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 6..454
NoneNo IPR availablePANTHERPTHR23315:SF253U-BOX DOMAIN-CONTAINING PROTEIN 9coord: 6..454
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 76..118
e-value: 2.56958E-23
score: 89.9854
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 72..148
IPR000225ArmadilloSMARTSM00185arm_5coord: 242..283
e-value: 14.0
score: 12.1
coord: 284..324
e-value: 0.098
score: 21.8
coord: 364..405
e-value: 130.0
score: 4.7
IPR000225ArmadilloPFAMPF00514Armcoord: 287..323
e-value: 6.3E-7
score: 29.2
IPR003613U box domainSMARTSM00504Ubox_2coord: 75..138
e-value: 6.8E-31
score: 118.6
IPR003613U box domainPFAMPF04564U-boxcoord: 73..142
e-value: 3.2E-23
score: 81.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 71..145
score: 40.886593
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 165..453
e-value: 2.5E-32
score: 114.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 71..145
e-value: 5.5E-28
score: 98.6
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 178..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g017140.1Lcy02g017140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity