Lcy02g016210 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g016210
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	beta-amyrin 11-oxidase-like
Location	Chr02: 43266344 .. 43273182 (+)
RNA-Seq Expression	Lcy02g016210
Synteny	Lcy02g016210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACTAATTTTATGGAGCCTTGATGGTATTTGATAACGAGATAACAATTGATCATTCAATGTAATAATTATCTTCTTCATCTATCTCAAAGCCACACCCTCTCGAGATGTTGTGAGGAGTCAAATAACAAAATATCATTTTTCGCCGACATGAACATAACTCAATTAGCAAAAAGTTTAGACTAATGACTAAGAGGTCTGTGATTCGAATCCTGATCAACTCCACATATGGTAAATCGTAAATAAAAAAAATAAATTACCAAATAGAAGCCAACTTGTTGCCATTAATGAGCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAATGATGAGCACTATTATTCTTAGCACTATAAAAGAGTCGCGGGTGTGGGTTTGGGAGATGCATCAGAAATCGAAGGAAGATGGAAATGAGCATTAATATTTGTTTGATTTTTGCAGGTGTTTTAGGGTTTTATGTGTTTGTGTTTGGGTTTGTGAGGAGACTGAACGAGCTATGGTATTTGGTTAAGTTGGGAAACAAGGTTTACAAATCTCTTCCTCCCGGTGACTTGGGATGGCCTCTCATTGGCTCTTCCCTATCCTTTTACAAAGCATTTACTGCTGGTGGTGATCCCGACTCTTTTATCAATCATCTTCTTTCCAGGTATCTCTTCATTAATGATCCTTCAACTTCTTGATATATACCTTCGATCAAGAGGTCAAAAGTCTGAATTCCTGTTGAACTAAAAGAAAAACTTGTTGGTAAATTCTCAACTTTTTAAAAAGTCTCAACCTTACCTAGTTTCTTTCTAAAAAAATTTATGTTAAAACTTGTTAAATAAAAAAATGAACCGATGATTTAAAGTATTATTGCTCTATTTTAGCGTATTTGAATGCGTGAAAGTTCCACTATGATACCATTTGACATGCTATCCATTCATAATAATTTAACGAAACACAAACTTTTTATTAATTTTATTTTTAACTTTTTTTTTTCTCGAAAGGACTTAATTGCCCCATTCTTCTTTCTGTTAGAAGAACGTGGAGGATAAATATGAATGGAGATGATTTTTCATTCAACTAAGCATGTCTTTTATAAGCTTACAAACATAACTATACAATACCAATGGTTCAAAACTTTAAATGTCATAAAATGCTGATAACTCACCCAGCTCCCAAAAACTCAAAAGTCAGGTTGCAAAGAAACCAAAAGCCAAAAGCCACAAAAACCAAAAGTCACATGAAATGCCACAAATAAAGTTTAATAATGAAAACCTTTCATTTTCTCAACACTTTCTTCTCTCTGCTCTTCGAGTCGAGCAATCATAATGGTGAACATTCTCCATGCAGAAATGGAAGAGTCGGGATGCACAAAAGCTACCTCTACGGGAGGCCAACGATCATCGTAACTGATCCAGATATATGTCGACGCATATACCTAGACGACGAAAGGTTCAAATCAAGTTATCCGAAGTCGGTGAAGCTATTAGAAGTAAATGGTTTTTTCTCAAGGACAGATCACAAATCTGCATATAGAATCATGGCAGCTCCAATGAATGGCTATGAAGTATTGTGTGGTTATGTGGAATTCATTGAGCAAATAATGGCAAAAGGGTTGGAGGAATGGAGCAGCATGAGAGAGCCCATTGAGTTGTTGACTGAGATTGGAAGTCTCTTCTTTAAAGTTATTGCACGTATTTTCTTGGGGAATGATATTGGTGGCTCTCGCTTGGCTGAGCTTGAGGCGTTTTACAAGCACTTGGGCCCTGCAATCTTGTCCATTGTTCCTTATGACTTGCCTGGTTTCACTTTTCACCGAGCACTCAAGGTATAAATATTCTCAACTGGCTTTTAACCCCGTAAAGATCGAACACGGGATCTTTGAAAAACTAGAGTATAACTCAACTGGTTAAGACATATTTCTTTAATCAAGTAATTAAGGTCAAAGGTTCAAAATAACTTTCGACTTCTAAATGTTGTATTGAACAACTAAAAAAATCACCAAAGATGATATGTTTTACTTGTGAGCTTCAAAATTGACACGTACAATATTATATGGGTTTGAACACATATCATGTATTATTATTATTGAACTCGAAAACTTTTGTTTTTTCAAAAAATATGTATCCATAATTTTGTGAGTAGTTATTCATCTTTACAAGGTTATAATAATCAATCACAAAGCTCTACCTAAATTTTTAATAGACACAAGGTTTTTTTTTTTTTTTTTTTGATAACGTGTAGGGTGGAGGGATTCAAATCACAAACCTCTTGATCATTAGTACAAGTTTTATGTTAGTTGAACTATAGTTACAAAGTTGAGAAGATTGAAAATTTGTTTTTGTTTTTCCTTTTTTCTTTTTTAAATTTATTTTCATCCACGCATTTAATGATTTACTTGTGCAAATCGATGTAATTGGGAAAAAACTTGGCTGCATACCGGTATGTAGGGATCTCTCTGCATACCACCATTAAAACATTGACAACTAAGAGAGATAACAAAAAATTCTCATTTTCTCCCTTTCTTTCTTACTTGTCAAGATTTTAATGGTATGTCCCTACATACCGGTATTCAGCCAAGTTTTGTTCTTGAAGATATATATTATCAAATGGGTGGTTGCATATATAGATTTTTTTTTAATATAACAAATGGGGTTATGAAGATTTGAACTTCCGACATCAAGGAAAGGAGTAATAATGCCCATTAGTCGTTACCGTACCGCTGAGTTATGCTCACTTTCATATATTTTACCCAAAATATTTTTACAAATTATATTTTGTACATGTTGGTAGGCAAGAAAAGAGATAGGAAATATCCTACAATGTGTGATAGAGGAGAAAAGAAGAGCACTAGAAAAGAAGGAAACAAGAGAAATGAAGTGTCAAATGGACAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATGATAATGCCATTATAGACTTGCTTCTTGGACTGTTACATGCTGGCCATTTTGCCTCAGCTCATGCTGCTATGTGGGCTCTTGTGCACATCTCAGAAAAATCTCATGTCTACCAAAAGGCCAAGGTGATTCTCTCAAAAGCACTTTTCCTCCTAGAAAAAAAAAAACAGAAAAAAAAAGAAGAAAACTTTAGTCCTTTTTCCCCTCTTTTGTAATTTTCAATTTTTCCATTTTTACAGGAAGAGCAAGAGTCGATTATAAAACAAAGACCATCTACTCAGAAGGGATTAACTTTTAAAGAAATCAAACAAATGAAATATCTCATGAAGGTGTGTGTGTGTTTTTTTTTTATTAATTATATATAATACTCGACGAAATCCACGTAATCTAATAAATAACATAGTTGACTTCTAAAAAGATAAAAGGCCAGATTGCGTTTGAGAGAAGTAAGGGTGTTTGGAATCCATTTCAATTTAAATGTTTTTTCAAGAATGCATTTTTTAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTTATATGTTTGGTTCATTTCTTCCAAATTGTTTTCATACACAAGTTATTGTATACTTTTTTTTTTTTTTTTGAGAAGGTAATTTATTGTATACTTAAAAAGTCTTTTTAAAAAAAAGTTGGTTTCAAGTGTTTCTCTTAAAATGATTTATTTATCAATCTCATTTTCTAATAAGCCATTTTTAGTGTTTGTCAAACACTAGAAATTTTTTTCAAAATTAAACCCTTGAAAAGTTAAACCAAACACACCCTAAATTGTAAATATTGACTAACTAAATGTAAGTTCTAAGAAAATATTAATTAACTGTTACGTGAAAAATAATCGAATGAAACATGTAAAACCACTCTTTTCAACAATTGGTTGATGCCTGCAAAAGTCAAAAAAAAAAAAAAAGAAAAAAAAAGGTAATTGAGTAGATATCGTTGAAAAAGAAAATAGCAATTATTATTGATTTCTCAAAGGAATAACAAAAAGAATTAAAAGAATTAGAAGAATAGTAATCTCCAAAATTGATAAGAAATAGTATTAAAGGGGCCACTCTAAGTTGTAGGATCATAGGGGATGTGGAGAAAAAAAAATTACAAATACTTTTTTCAAACTATATGTCAAGATGCATTCCTAAAAAAAAAAAAAAAAAAGCTTTTGAGAAAAATAAACTATAAATATCCCTCTCAAACCAAATGTCAACATATTCTAAAGAAACTTAGAAATTATTAATTAAGGGAGAATAACTGTCAACATGAACATAGTTTAACGATTAAGACATTTACTATTTTTTCTTGAGAATGGAGGTTCAAATCTCCACCCTATATTTGTTGTACTAATCAAAGTGATGATGACTTATGGAATAAGTTAAAACCATTCTCCACTACCTACAAAGCCTAAAAACGTTAGCTAGATAGATAGCAACAACTCGGTTATAAAGATAGCGAATATACATTATTACTCTAAGGAAATACCTTTTAAAAAAAATTAATGTTTCTATAAGAACAAAAAATTCCTCCATAATCTGTTGTTTGAAATTGGTAACATATAATACAAAAGGCTAGTCCAAATATGACATAGGACTTTACATGACAAACTAGAATTATAAAGGGGTTTTTTTTTTTTGTCATGGAAGATTTGGCCTCTTCATAAGTCATCTCATTCTTATCAATGTGTGACAACAATGATGGCAAAAAAAAGTGACAAAAAATAATATTAATAAATTTAATATCGAGAGCGAGAGACACCAACCCAAAACTTTGCAGCGATGCTAACACAAGCAAAATGTCATATAACCTTTGTTTTAAAAATTAAGCAGGTCATATATCAATTTGTTTGAATTTTTTTTTAATTTTTTTTTTATATATGTATAGTCCTTGAAACTTATGTTAATTGAATAAAATATAATACAACCAACCTTGATTTGATTTATTACTTGTGTTTCAGTTCATTAACGAGCTATTGAGAAGAAATACCATCTCTGTTGCAAGTTTTCGTGAGGCAAAAACGGATGTAAACATCAATGGTTAGTTTAGGCCCCATTTGATAACTATTTTATTTTTTGTTTTTAGTTTTTGAAAATTATGTTTGTTTCGTCTCAAATTTCTTACAATGATGTTCATCTTTTTTAAGGATACATTTGAATTCATAGCCAAATTCTAAAAACAAAAATAAGTTTTTGGAAACTACTTTTTCTAGTTTTTAAAATTTGACTCAGTTTTTGAAAACGAGGAAAGGATGTAGATAGTAAAACAAAGAAACTTGTGGGTAAAAGTAGAGTTTTAGGCTTAATTTTCAAAAACAAAAAACCAAAAACGAAATGGTTATCAAACGGGGCCTTGGTTATTCTATCATTGTCTTTGTCATTATATATTCATTTCCCTTTTTAAAATTCTTGTTAGATATAATGCTATATATATTTTTTTATTAGCTCGTATAATTGCATGATGCCGATTATTTCTTCGTTGATAATAATTTATTTTGTTCTTTTTAACTATTATTTTGGTCTTTTTTTTTTTTTTTAATTTTTCTATTTGTTTTAGGCTATACTATACCAAAAGGATGGAGTGTACCAATATGGACTAGAGCTGTTCACATGGACCCTCAAATATATTCAAATCCGCAAGAATTTGATCCTTCGAGATGGGATGTATGTTTCATAATCCCAATATAATCAATTCATTAAAATAAATCAACATGAATCTATATATAAAAAGGTTAAATTATAAAAAATACCCTTGAACTATGGAGTTTGTTTAAAAAATGCCCCTGAACTTTAAAAAGTTTCAATAATACCCCTGAACTTTACAAAACGTTTCAAAAATACCCTTGTAGTTAATTTTTGGATGGGAACCGTTAAAACCGTTAAAAATGTTTCGAAAATACCATTGTCGAAATCTAGTCAAGCTCTTGGGATGATGTGTTGAAAGAGTAGAAAAACTCCTAGTGTATGAATACATGAAAAAATAGGTAAAGCTTCTCATTAATTTTCATGGAAGAAGAATTTACGGTGAGAGAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACATTTTCTAATAAGGTTATTTTTTAAAATCTTTTGAAAGTTAAAGAGTATTAATGAAACTGAAATTTTTAATGGTTTTCATCCAAAAATTAATGACAAGGGTATTTTTGAAACATTTTTTAATGTTCAGGGGTATTTTTGAAACTTTTTAAAGGTCAGAGACATTTTTTAAACAAACCCCATAGTTTAGGGACATTTTTTATAATTTAGCCTTAAAAAAAAAGTTAGTTTTCATATTTATTTATTTATTTTTGAAATCAAATCGTATCAAGTCTAGGATTTCAAAAGTAGATATCGCCCATTTTTAAGAGTGTTGACAAATAAGAACGTCTTTTAAAGCCAAAGTGAACCTAGCTCAAAGCAGCTTGTACTAATGATCAAAAGGTTTGTGGTTTAAATCTCTCACTCCACACAATTTTAAAAAGAAAAAAAAAATCATCATGAATTATGAAATAGTCCTTAATTTAAATAAAAATTAAAAATATTAATGTTGGTAGAATTTGGGAAATGTGATGGGCAGAATTATACACCAAAACCAGGAGCATTCATCCCTTTTGGAATTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTACAAAGCTTGAGATTACAATTTTACTTCATCATTTTATACTCAATTACAAGTAAGTATCGTTCTTGCTTTTTTTTTTTTGTTACATTGAAAAAAAAAAAAAGTGATGGATTTTTTTTTTTGGTATTGTTGTAGAATGGAACGTGTCAATCCAAAAAGCTTGATCACTCACTTACCAGCCCCCAAACCAATAGACAACTGCCTTTGCAAAATTATCAAGCGCCCATAG

mRNA sequence

ATGGTACTAATTTTATGGAGCCTTGATGGTGTTTTAGGGTTTTATGTGTTTGTGTTTGGGTTTGTGAGGAGACTGAACGAGCTATGGTATTTGGTTAAGTTGGGAAACAAGGTTTACAAATCTCTTCCTCCCGGTGACTTGGGATGGCCTCTCATTGGCTCTTCCCTATCCTTTTACAAAGCATTTACTGCTGGTGGTGATCCCGACTCTTTTATCAATCATCTTCTTTCCAGAAATGGAAGAGTCGGGATGCACAAAAGCTACCTCTACGGGAGGCCAACGATCATCGTAACTGATCCAGATATATGTCGACGCATATACCTAGACGACGAAAGGTTCAAATCAAGTTATCCGAAGTCGGTGAAGCTATTAGAAGTAAATGGTTTTTTCTCAAGGACAGATCACAAATCTGCATATAGAATCATGGCAGCTCCAATGAATGGCTATGAAGTATTGTGTGGTTATGTGGAATTCATTGAGCAAATAATGGCAAAAGGGTTGGAGGAATGGAGCAGCATGAGAGAGCCCATTGAGTTGTTGACTGAGATTGGAAGTCTCTTCTTTAAAGTTATTGCACGTATTTTCTTGGGGAATGATATTGGTGGCTCTCGCTTGGCTGAGCTTGAGGCGTTTTACAAGCACTTGGGCCCTGCAATCTTGTCCATTGTTCCTTATGACTTGCCTGGTTTCACTTTTCACCGAGCACTCAAGGAGAAAAGAAGAGCACTAGAAAAGAAGGAAACAAGAGAAATGAAGTGTCAAATGGACAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATGATAATGCCATTATAGACTTGCTTCTTGGACTGTTACATGCTGGCCATTTTGCCTCAGCTCATGCTGCTATGTGGGCTCTTGTGCACATCTCAGAAAAATCTCATGTCTACCAAAAGGCCAAGGCTATACTATACCAAAAGGATGGAGTGTACCAATATGGACTAGAGCTGTTCACATGGACCCTCAAATATATTCAAATCCGCAAGAATTTGATCCTTCGAGATGGGATAATGGAACGTGTCAATCCAAAAAGCTTGATCACTCACTTACCAGCCCCCAAACCAATAGACAACTGCCTTTGCAAAATTATCAAGCGCCCATAG

Coding sequence (CDS)

ATGGTACTAATTTTATGGAGCCTTGATGGTGTTTTAGGGTTTTATGTGTTTGTGTTTGGGTTTGTGAGGAGACTGAACGAGCTATGGTATTTGGTTAAGTTGGGAAACAAGGTTTACAAATCTCTTCCTCCCGGTGACTTGGGATGGCCTCTCATTGGCTCTTCCCTATCCTTTTACAAAGCATTTACTGCTGGTGGTGATCCCGACTCTTTTATCAATCATCTTCTTTCCAGAAATGGAAGAGTCGGGATGCACAAAAGCTACCTCTACGGGAGGCCAACGATCATCGTAACTGATCCAGATATATGTCGACGCATATACCTAGACGACGAAAGGTTCAAATCAAGTTATCCGAAGTCGGTGAAGCTATTAGAAGTAAATGGTTTTTTCTCAAGGACAGATCACAAATCTGCATATAGAATCATGGCAGCTCCAATGAATGGCTATGAAGTATTGTGTGGTTATGTGGAATTCATTGAGCAAATAATGGCAAAAGGGTTGGAGGAATGGAGCAGCATGAGAGAGCCCATTGAGTTGTTGACTGAGATTGGAAGTCTCTTCTTTAAAGTTATTGCACGTATTTTCTTGGGGAATGATATTGGTGGCTCTCGCTTGGCTGAGCTTGAGGCGTTTTACAAGCACTTGGGCCCTGCAATCTTGTCCATTGTTCCTTATGACTTGCCTGGTTTCACTTTTCACCGAGCACTCAAGGAGAAAAGAAGAGCACTAGAAAAGAAGGAAACAAGAGAAATGAAGTGTCAAATGGACAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATGATAATGCCATTATAGACTTGCTTCTTGGACTGTTACATGCTGGCCATTTTGCCTCAGCTCATGCTGCTATGTGGGCTCTTGTGCACATCTCAGAAAAATCTCATGTCTACCAAAAGGCCAAGGCTATACTATACCAAAAGGATGGAGTGTACCAATATGGACTAGAGCTGTTCACATGGACCCTCAAATATATTCAAATCCGCAAGAATTTGATCCTTCGAGATGGGATAATGGAACGTGTCAATCCAAAAAGCTTGATCACTCACTTACCAGCCCCCAAACCAATAGACAACTGCCTTTGCAAAATTATCAAGCGCCCATAG

Protein sequence

MVLILWSLDGVLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFFSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKEKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMWALVHISEKSHVYQKAKAILYQKDGVYQYGLELFTWTLKYIQIRKNLILRDGIMERVNPKSLITHLPAPKPIDNCLCKIIKRP

Homology

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BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 129/321 (40.19%), Postives = 200/321 (62.31%), Query Frame = 0


Query: 15  YVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINH 74
           Y+F   FVR LN  +Y VKL  K +  LPPGD+GWPLIG  LSF K F++ G PDSFIN+
Sbjct: 17  YIFGSKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDFSS-GHPDSFINN 76

Query: 75  LLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVK-------LLEVN 134
           L+ + GR G++K++L+G P+IIV +P +CRR+  DD  FK  YPKS+K       +++V 
Sbjct: 77  LVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDV- 136

Query: 135 GFFSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLF 194
              S  +H+   R++ +P+ G++ L  Y+E +E+I+   LEE SSM+ P+ELL E+  + 
Sbjct: 137 ---SNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVS 196

Query: 195 FKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK---------- 254
           FK I  +F+G+      + ++ + +  L   + SI P ++PGFTFH+AL+          
Sbjct: 197 FKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEARKKLAKIVQ 256

Query: 255 ----EKRRALEK--KETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASA 313
               E+R  +E   +E  + K  +D L+   DENG KL D++ I DLL+GLL AGH ++A
Sbjct: 257 PVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-ISDLLIGLLFAGHESTA 316

HSP 2 Score: 44.3 bits (103), Expect = 3.6e-03
Identity = 20/41 (48.78%), Postives = 27/41 (65.85%), Query Frame = 0


Query: 336 IQIRKNLILRDGIMERVNPKSLITHLPAPKPIDNCLCKIIK 377
           I I  +  LR+  +ER+NP+  +T LP  KP DNCL K+IK
Sbjct: 449 ISIFLHYFLRNYRLERINPECHVTSLPVSKPTDNCLAKVIK 489

[Close]

BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 6.5e-53
Identity = 128/328 (39.02%), Postives = 196/328 (59.76%), Query Frame = 0


Query: 2   VLILWSLDGVLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKA 61
           ++++W    +LG +V  +  ++R+N   Y+ KLG K +  LPPGDLGWP+IG+  SF +A
Sbjct: 6   LILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRA 65

Query: 62  FTAGGDPDSFINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKS- 121
           F    DP+SFI   ++R GR G++K++++G P ++VT P+ CRR+  DD+ F   +PKS 
Sbjct: 66  FKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 125

Query: 122 VKLLEVNGF--FSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIE 181
           +KL+    F   S  +HK   R+ +AP+NG E L  Y++FIE+ +   LE+WS M E IE
Sbjct: 126 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IE 185

Query: 182 LLTEIGSLFFKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKE 241
            L+ +  L FKVI  IFL ++     +  LE  Y +L   + + +  +LPGF +HRALK 
Sbjct: 186 FLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKA 245

Query: 242 KRRAL------------EKKE--TREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLH 301
           +++ +            ++K+  +   K  +D LI   DENG ++ DD  IIDLLL  L+
Sbjct: 246 RKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLN 305

Query: 302 AGHFASAHAAMWALVHISEKSHVYQKAK 313
           AGH +S H  MWA + + E   + QKAK
Sbjct: 306 AGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.7e-50
Identity = 123/310 (39.68%), Postives = 177/310 (57.10%), Query Frame = 0


Query: 22  VRRLNELWYLVKLG-NKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNG 81
           VR++N   Y   LG N+ Y  LPPGDLGWP IG+ LSF +AF    DPDSF   L+ R G
Sbjct: 26  VRKVNVWLYESSLGENRHY--LPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYG 85

Query: 82  RVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKS-VKLLEVNGF--FSRTDHKS 141
             G++K++++G P+IIVT  D CRR+  DD+ FK  +P S ++L+    F   S  +HK 
Sbjct: 86  PKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKR 145

Query: 142 AYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKVIARIFLG 201
             R+ AAP+NG+E L  Y+ +IE+ +   L++W+ M E  E LT +  L F++I  IFL 
Sbjct: 146 LRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLS 205

Query: 202 NDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK--------------EKRRAL 261
           ++   + +  LE  Y  L   + ++   ++PGF +HRALK              E+R   
Sbjct: 206 SE-SENVMDALEREYTALNYGVRAMA-VNIPGFAYHRALKARKTLVAAFQSIVTERRNQR 265

Query: 262 EKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMWALVHISE 314
           ++      K  +D L+   DE+G K  DD  IID+LL  L+AGH +S H  MWA V + E
Sbjct: 266 KQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQE 325

[Close]

BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.7e-48
Identity = 114/329 (34.65%), Postives = 186/329 (56.53%), Query Frame = 0


Query: 5   LWSL-DGVLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFT 64
           LW L   V+   V V   VRR ++   +  LG +    LPPG++GWP++GS  +F +AF 
Sbjct: 17  LWVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK 76

Query: 65  AGGDPDSFINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPK-SVK 124
           + G+PD+FI   + R GR G+++++++  PTI+   P+ C+++ +DDE F + +PK +V 
Sbjct: 77  S-GNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 136

Query: 125 LLEVNGF--FSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMRE-PIEL 184
           L+    F   S  DH+   ++ AAP+NG++ L  Y+ FI+Q +   L  WSS     +E 
Sbjct: 137 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 196

Query: 185 LTEIGSLFFKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKEK 244
           LTE+  + FK+I +IF+ +    + +  LE  Y  L   + ++   +LPGF ++RAL+ +
Sbjct: 197 LTELRRMTFKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMA-INLPGFAYYRALRAR 256

Query: 245 RR-------ALEKKETREMK--------CQMDKLIVATDENGTKLYDDNAIIDLLLGLLH 304
           R+        L+ +     K          MD+LI A DE G +L DD  I+D+L+  L+
Sbjct: 257 RKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYLN 316

Query: 305 AGHFASAHAAMWALVHISEKSHVYQKAKA 314
           AGH +S H  MWA V + E   ++ +AKA
Sbjct: 317 AGHESSGHITMWATVFLQENPDIFARAKA 341

[Close]

BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.4e-47
Identity = 110/313 (35.14%), Postives = 173/313 (55.27%), Query Frame = 0


Query: 22  VRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNGR 81
           VR  +  ++   LG      LPPGD+GWPL+G   +F +AF + G PDSFI+    R GR
Sbjct: 25  VRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKS-GRPDSFIDSFARRFGR 84

Query: 82  VGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPK-SVKLLEVNGFFSR--TDHKSA 141
            G+++++++  PTI+ T P+ C+++ +DD+ F + +PK +V L+    F +    +H+  
Sbjct: 85  AGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRL 144

Query: 142 YRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREP--IELLTEIGSLFFKVIARIFL 201
            ++ AAP+NG++ L  Y+ FI+  +   L  WS        E LTE+  + F++I +IF+
Sbjct: 145 RKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFM 204

Query: 202 GNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKEKR-------RALEKKETR 261
           G        AELE  Y  L   + ++   DLPGF +H+A++ +R       R L+++  R
Sbjct: 205 GG-ADERTAAELERTYTELNYGMRAMA-IDLPGFAYHKAIRARRRLVAALQRVLDERRAR 264

Query: 262 EMKC---------QMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMWALVH 314
             K           MD+LI   DE G +L DD  IID+L+  L+AGH +S H  MWA V 
Sbjct: 265 GGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDE-IIDVLVMYLNAGHESSGHITMWATVF 324

[Close]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.4e-137
Identity = 276/479 (57.62%), Postives = 316/479 (65.97%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFY AFT GGDPDS
Sbjct: 9   IIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+ YGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNGFF
Sbjct: 69  FIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           +R DHKSAYRIMAAPMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 TRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYDLPG TFH ALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEK+E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISEK HV+ KAK    +I+ Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVA 368

Query: 371 ---------------------------------------------------------LRD 379
                                                                    L  
Sbjct: 369 SFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSPKPGTFLPF 428

[Close]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.3e-136
Identity = 271/479 (56.58%), Postives = 314/479 (65.55%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++G  + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFY AFT GGDPDS
Sbjct: 9   IIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+LYGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNGFF
Sbjct: 69  FIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           +R DHKSAYRIMAAPMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 TRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYD+PG  +HRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKIENVLECVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALE+KETRE+  QMDKLI+A DENG KLYD+N IIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISEK H++ KAK    +IL Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIVVA 368

Query: 371 ----------LRDGI--------------------------------------------- 379
                     + D I                                             
Sbjct: 369 SFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSPKPGTFIPF 428

[Close]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL
Match: A0A6J1GKS7 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454913 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 4.1e-135
Identity = 266/452 (58.85%), Postives = 313/452 (69.25%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAF+ GGD DS
Sbjct: 9   IIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFSVGGDSDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLL+RNGRVGM+KS+LYG+PTIIVTDP+ICRRIYLDDE FK +YP SVK+LEVNG F
Sbjct: 69  FIYHLLTRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFKPNYPNSVKILEVNGLF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           SR DHK AYRIMAAPMNGYEVL  YV+FI+QIMA+GLE+WS+ +EPIELL EIG LFFKV
Sbjct: 129 SRMDHKCAYRIMAAPMNGYEVLSHYVDFIDQIMAEGLEKWSTKKEPIELLGEIGRLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYDLPGFTFHRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGFTFHRALKARKKIENILQCVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKR+ALEK+E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGLLHAGH   ++AAMW
Sbjct: 249 EEKRKALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPSYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHIS+K H++ KAK    +IL Q+     G+    ++   + +K I   +R+N I   
Sbjct: 309 ALVHISQKPHIFHKAKEEQESILQQRLSTQKGLNFQEIKQMKYLIKLINEVLRRNTIAVA 368

Query: 371 ------------------------------LRDGI------------------------- 379
                                         +  GI                         
Sbjct: 369 SFRKAKKDVNINDYTIPKGWNNYSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILN 428

[Close]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.1e-132
Identity = 269/479 (56.16%), Postives = 310/479 (64.72%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAF  GGDPDS
Sbjct: 9   IIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+LYG PTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNG F
Sbjct: 69  FIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGLF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           SR DHK AYRIMAAP+NGY VL  YV+FI+QIMAKGLEEWS+ +EPIEL  EIGSLFF+V
Sbjct: 129 SRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGEIGSLFFRV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYDLPG TFHRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKIENILQCVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEK+E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGLLH GH   A+AAMW
Sbjct: 249 EEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQKD----GVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISE  HV+ KAK    +IL Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVA 368

Query: 371 ---------------------------------------------------------LRD 379
                                                                    +  
Sbjct: 369 SFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWENHSPKPGTFIPF 428

[Close]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 8.9e-130
Identity = 264/488 (54.10%), Postives = 309/488 (63.32%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAFT GGDPDS
Sbjct: 9   IIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+LYG+PTIIVTDP+ICRRIYLDDE F+ +YPKSVK+LEVNG F
Sbjct: 69  FIYHLLSRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFQPNYPKSVKILEVNGLF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           SR DH  AYRIMAAP+NGY VL  YV+FI+QIMAKGLE+WS+ +EPIELL EIGSLFFKV
Sbjct: 129 SRMDHNCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEKWSTKKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYD+PG TFHRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLTFHRALKARKKIENILQCVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEK+E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGLLH GH   A+AAMW
Sbjct: 249 EEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALV I E  HV+ KAK    +IL Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVQIFETPHVFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVA 368

Query: 371 ------------------------------------------------------------ 379
                                                                       
Sbjct: 369 SFREAKTDININGELFYYIVLDYTIPKGWTVLIWTRAIHMDPQLYSNPKEFDPSRWENHS 428

[Close]

BLAST of Lcy02g016210 vs. NCBI nr
Match: XP_023511634.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504.2 bits (1297), Expect = 9.7e-139
Identity = 275/452 (60.84%), Postives = 315/452 (69.69%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAFT GGDPDS
Sbjct: 9   IIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLL+RNGRVGM+KS+LYGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNG F
Sbjct: 69  FIYHLLTRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGLF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           SR DHKSAYRIM APMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 SRIDHKSAYRIMVAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYDLPG TFHRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKIENILECVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEK+E RE++ QMDKLI+A DE   KLYD+NAIIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEKEE-REVESQMDKLIMARDEKWMKLYDNNAIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISEK HV+ KAK    +I+ Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISEKLHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVA 368

Query: 371 ------------------------------LRDGI------------------------- 379
                                         L  GI                         
Sbjct: 369 SFRKAKKDVNINDYTIPKGWNNYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILN 428

[Close]

BLAST of Lcy02g016210 vs. NCBI nr
Match: XP_022952843.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 497.7 bits (1280), Expect = 9.1e-137
Identity = 276/479 (57.62%), Postives = 316/479 (65.97%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++GF + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFY AFT GGDPDS
Sbjct: 9   IIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+ YGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNGFF
Sbjct: 69  FIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           +R DHKSAYRIMAAPMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 TRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYDLPG TFH ALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEK+E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISEK HV+ KAK    +I+ Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVA 368

Query: 371 ---------------------------------------------------------LRD 379
                                                                    L  
Sbjct: 369 SFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSPKPGTFLPF 428

[Close]

BLAST of Lcy02g016210 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 496.1 bits (1276), Expect = 2.6e-136
Identity = 271/479 (56.58%), Postives = 314/479 (65.55%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++G  + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFY AFT GGDPDS
Sbjct: 9   IIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+LYGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNGFF
Sbjct: 69  FIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           +R DHKSAYRIMAAPMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 TRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYD+PG  +HRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKIENVLECVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALE+KETRE+  QMDKLI+A DENG KLYD+N IIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI--- 370
           ALVHISEK H++ KAK    +IL Q+     G+    ++   + +K+I   +R+N I   
Sbjct: 309 ALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIVVA 368

Query: 371 ----------LRDGI--------------------------------------------- 379
                     + D I                                             
Sbjct: 369 SFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSPKPGTFIPF 428

[Close]

BLAST of Lcy02g016210 vs. NCBI nr
Match: KAG6571835.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494.2 bits (1271), Expect = 1.0e-135
Identity = 260/407 (63.88%), Postives = 295/407 (72.48%), Query Frame = 0


Query: 21  FVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNG 80
           FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAFT GGDPDSFI HLLSRNG
Sbjct: 2   FVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVGGDPDSFIYHLLSRNG 61

Query: 81  RVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFFSRTDHKSAYR 140
           RVGM+KS+LYG PTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNG FSR DHK AYR
Sbjct: 62  RVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGLFSRMDHKCAYR 121

Query: 141 IMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKVIARIFLGNDI 200
           IMAAP+NGY VL  YV+FI+QIM KGLE WS+ +EPIEL  EIGSLFF+VI+RIFLG DI
Sbjct: 122 IMAAPINGYNVLSHYVDFIDQIMTKGLEGWSTKKEPIELFDEIGSLFFRVISRIFLGTDI 181

Query: 201 GGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK--------------EKRRALEKK 260
           G SR+AELE  YK LGPAILSI+PYDLPG TFHRALK              EKRRALEK+
Sbjct: 182 GASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKIENILQCVIEEKRRALEKQ 241

Query: 261 ETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMWALVHISEKSH 320
           E RE++ QMDKLI+A DENG KLYD+NAIIDLLLGL H GH   A+AAMWALVHISE  H
Sbjct: 242 E-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLQHGGHHTPAYAAMWALVHISETPH 301

Query: 321 VYQKAK----AILYQKD----GVYQYGLELFTWTLKYIQIRKNLILRDGI---------- 379
           V+ KAK    +IL Q+     G+    ++   + +K    +    +  GI          
Sbjct: 302 VFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKNHSPKPGTFIPFGIGARFCPGSEL 361

[Close]

BLAST of Lcy02g016210 vs. NCBI nr
Match: KAG6571834.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-135
Identity = 250/357 (70.03%), Postives = 289/357 (80.95%), Query Frame = 0


Query: 11  VLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDS 70
           ++G  + V+ FVR+LNE+WYLVKLG K YKSLPPGD+GWPL+GSS SFYKAFT GGDPDS
Sbjct: 9   IIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVGGDPDS 68

Query: 71  FINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKSVKLLEVNGFF 130
           FI HLLSRNGRVGM+KS+LYGRPTIIVTDP+ICRRIYLDDE FK +YPKSVK+LEVNGFF
Sbjct: 69  FIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFF 128

Query: 131 SRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKV 190
           +R DHKSAYRIMAAPMNGYEVL  YV+FI+Q+MAKGLEEWSSM+EPIELL EIGSLFFKV
Sbjct: 129 TRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV 188

Query: 191 IARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK------------- 250
           I+RIFLG DIG SR+AELE  YK LGPAILSI+PYD+PG  +HRALK             
Sbjct: 189 ISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKIENVLECVI 248

Query: 251 -EKRRALEKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMW 310
            EKRRALEKKETRE+  QMDKLI+A DENG KLYD+N IIDLLLGLLHAGH   A+AAMW
Sbjct: 249 EEKRRALEKKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMW 308

Query: 311 ALVHISEKSHVYQKAK----AILYQK----DGVYQYGLELFTWTLKYIQ--IRKNLI 344
           ALVHISEK H++ KAK    +IL Q+     G+    ++   + +K+I   +R+N I
Sbjct: 309 ALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTI 365

[Close]

BLAST of Lcy02g016210 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 209.5 bits (532), Expect = 4.6e-54
Identity = 128/328 (39.02%), Postives = 196/328 (59.76%), Query Frame = 0


Query: 2   VLILWSLDGVLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKA 61
           ++++W    +LG +V  +  ++R+N   Y+ KLG K +  LPPGDLGWP+IG+  SF +A
Sbjct: 6   LILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRA 65

Query: 62  FTAGGDPDSFINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKS- 121
           F    DP+SFI   ++R GR G++K++++G P ++VT P+ CRR+  DD+ F   +PKS 
Sbjct: 66  FKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 125

Query: 122 VKLLEVNGF--FSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIE 181
           +KL+    F   S  +HK   R+ +AP+NG E L  Y++FIE+ +   LE+WS M E IE
Sbjct: 126 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IE 185

Query: 182 LLTEIGSLFFKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKE 241
            L+ +  L FKVI  IFL ++     +  LE  Y +L   + + +  +LPGF +HRALK 
Sbjct: 186 FLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKA 245

Query: 242 KRRAL------------EKKE--TREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLH 301
           +++ +            ++K+  +   K  +D LI   DENG ++ DD  IIDLLL  L+
Sbjct: 246 RKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLN 305

Query: 302 AGHFASAHAAMWALVHISEKSHVYQKAK 313
           AGH +S H  MWA + + E   + QKAK
Sbjct: 306 AGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of Lcy02g016210 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 209.5 bits (532), Expect = 4.6e-54
Identity = 128/328 (39.02%), Postives = 196/328 (59.76%), Query Frame = 0


Query: 2   VLILWSLDGVLGFYVFVFGFVRRLNELWYLVKLGNKVYKSLPPGDLGWPLIGSSLSFYKA 61
           ++++W    +LG +V  +  ++R+N   Y+ KLG K +  LPPGDLGWP+IG+  SF +A
Sbjct: 6   LILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRA 65

Query: 62  FTAGGDPDSFINHLLSRNGRVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKS- 121
           F    DP+SFI   ++R GR G++K++++G P ++VT P+ CRR+  DD+ F   +PKS 
Sbjct: 66  FKT-SDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 125

Query: 122 VKLLEVNGF--FSRTDHKSAYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIE 181
           +KL+    F   S  +HK   R+ +AP+NG E L  Y++FIE+ +   LE+WS M E IE
Sbjct: 126 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IE 185

Query: 182 LLTEIGSLFFKVIARIFLGNDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKE 241
            L+ +  L FKVI  IFL ++     +  LE  Y +L   + + +  +LPGF +HRALK 
Sbjct: 186 FLSHLRKLTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKA 245

Query: 242 KRRAL------------EKKE--TREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLH 301
           +++ +            ++K+  +   K  +D LI   DENG ++ DD  IIDLLL  L+
Sbjct: 246 RKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLN 305

Query: 302 AGHFASAHAAMWALVHISEKSHVYQKAK 313
           AGH +S H  MWA + + E   + QKAK
Sbjct: 306 AGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of Lcy02g016210 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 199.5 bits (506), Expect = 4.8e-51
Identity = 123/310 (39.68%), Postives = 177/310 (57.10%), Query Frame = 0


Query: 22  VRRLNELWYLVKLG-NKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNG 81
           VR++N   Y   LG N+ Y  LPPGDLGWP IG+ LSF +AF    DPDSF   L+ R G
Sbjct: 26  VRKVNVWLYESSLGENRHY--LPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYG 85

Query: 82  RVGMHKSYLYGRPTIIVTDPDICRRIYLDDERFKSSYPKS-VKLLEVNGF--FSRTDHKS 141
             G++K++++G P+IIVT  D CRR+  DD+ FK  +P S ++L+    F   S  +HK 
Sbjct: 86  PKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKR 145

Query: 142 AYRIMAAPMNGYEVLCGYVEFIEQIMAKGLEEWSSMREPIELLTEIGSLFFKVIARIFLG 201
             R+ AAP+NG+E L  Y+ +IE+ +   L++W+ M E  E LT +  L F++I  IFL 
Sbjct: 146 LRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLS 205

Query: 202 NDIGGSRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALK--------------EKRRAL 261
           ++   + +  LE  Y  L   + ++   ++PGF +HRALK              E+R   
Sbjct: 206 SE-SENVMDALEREYTALNYGVRAMA-VNIPGFAYHRALKARKTLVAAFQSIVTERRNQR 265

Query: 262 EKKETREMKCQMDKLIVATDENGTKLYDDNAIIDLLLGLLHAGHFASAHAAMWALVHISE 314
           ++      K  +D L+   DE+G K  DD  IID+LL  L+AGH +S H  MWA V + E
Sbjct: 266 KQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQE 325

[Close]

BLAST of Lcy02g016210 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 95.9 bits (237), Expect = 7.4e-20
Identity = 86/297 (28.96%), Postives = 148/297 (49.83%), Query Frame = 0


Query: 28  LWYLVK-LGNKVYKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSRNGRVGMHK 87
           L++L K L N  Y +LPPG +G+PLIG +LSF  A    G P+ F+   + R+   G+ K
Sbjct: 18  LFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSA-GRQGHPEKFVTDRV-RHFSSGIFK 77

Query: 88  SYLYGRPTIIVTDPDICRRIYLDDERFKSS-YPKSV-KLLEVNGFFSRTDHKSAYRIMAA 147
           ++L+G P  +VT     + ++ ++ +   S +P SV K+   +   S  +     R++  
Sbjct: 78  THLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTSSKEEAIKTRMLLM 137

Query: 148 PMNGYEVLCGYVEFIEQIMAKGLE-EWSSMREPIEL-LTEIGSLFFKVIARIFLGNDIGG 207
           P    E L  YV  +++I  K  E EW++  + I   LT+     F +  R+FL  D   
Sbjct: 138 PSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTK--KFTFSIACRLFLSMD-DL 197

Query: 208 SRLAELEAFYKHLGPAILSIVPYDLPGFTFHRALKEKRRALEKKET----REMKCQMDKL 267
            R+ +LE  +  +   + SI P DLPG  F+RA+K  R   ++  T    R+ + +  K+
Sbjct: 198 ERVRKLEEPFTTVMTGVFSI-PIDLPGTRFNRAIKASRLLSKEVSTIIRQRKEELKAGKV 257

Query: 268 IVATDENGTKLYD-----DNAIIDLLLGLLHAGHFASAHAAMWALVHISEKSHVYQK 311
            V  D     L +     D  + D ++ LL  GH  ++    + + +++E  H+YQ+
Sbjct: 258 SVEQDILSHMLMNIGETKDEDLADKIIALLIGGHDTTSIVCTFVVNYLAEFPHIYQR 308

[Close]

BLAST of Lcy02g016210 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 90.5 bits (223), Expect = 3.1e-18
Identity = 87/301 (28.90%), Postives = 145/301 (48.17%), Query Frame = 0


Query: 39  YKSLPPGDLGWPLIGSSLSFYKAFTAGGDPDSFINHLLSR--NGRVGMHKSYLYGRPTII 98
           Y +LPPG+ G PLIG S SF  A    G P+ FI   + R  +    + K++L+G PT +
Sbjct: 31  YPNLPPGNTGLPLIGESFSFLSA-GRQGHPEKFITDRVRRFSSSSSCVFKTHLFGSPTAV 90

Query: 99  VTDPDICRRIYLDDERFKSS-YPKSV-KLLEVNGFFSRTDHKSAYRIMAAPMNGYEVLCG 158
           VT     + ++ ++ +   S +P SV K+   +   S  +     R++ +     E L  
Sbjct: 91  VTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQTSSKEEARKLRMLLSQFMKPEALRR 150

Query: 159 YVEFIEQIMAKGLE-EWSSMREPIEL-LTEIGSLFFKVIARIFLGNDIGGSRLAELEAFY 218
           YV  +++I  +  E EW++  + I   LT+     F +  R FL  +   +R+ +LE  +
Sbjct: 151 YVGVMDEIAQRHFETEWANQDQVIVFPLTK--KFTFSIACRSFLSME-DPARVRQLEEQF 210

Query: 219 KHLGPAILSIVPYDLPGFTFHRALKEKRRALEKKET------REMKCQMDKLIVATDENG 278
             +   I SI P DLPG  F+RA+K  R  L +KE       R+ + +  K +   D   
Sbjct: 211 NTVAVGIFSI-PIDLPGTRFNRAIKASR--LLRKEVSAIVRQRKEELKAGKALEEHDILS 270

Query: 279 TKLYD-----DNAIIDLLLGLLHAGHFASAHAAMWALVHISEKSHVYQKA----KAILYQ 319
             L +     D  + D ++GLL  GH  ++    + + +++E  HVYQ+     K IL +
Sbjct: 271 HMLMNIGETKDEDLADKIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKE 324

The following BLAST results are available for this feature:

BLAST of Lcy02g016210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.1e-57	40.19	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	6.5e-53	39.02	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	6.7e-50	39.68	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	3.7e-48	34.65	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9AXH9	2.4e-47	35.14	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of Lcy02g016210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GLR1	4.4e-137	57.62	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1GJM1	1.3e-136	56.58	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A6J1GKS7	4.1e-135	58.85	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454913 PE=3 S...	[more]
A0A6J1GJP8	1.1e-132	56.16	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 S...	[more]
A0A6J1I8I5	8.9e-130	54.10	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=...	[more]

BLAST of Lcy02g016210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023511634.1	9.7e-139	60.84	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952843.1	9.1e-137	57.62	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_022952147.1	2.6e-136	56.58	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
KAG6571835.1	1.0e-135	63.88	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6571834.1	2.2e-135	70.03	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lcy02g016210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	4.6e-54	39.02	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	4.6e-54	39.02	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	4.8e-51	39.68	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT5G36140.1	7.4e-20	28.96	cytochrome P450, family 716, subfamily A, polypeptide 2	[more]
AT5G36110.1	3.1e-18	28.90	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 43..214 e-value: 2.8E-5 score: 23.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 34..323 e-value: 1.8E-32 score: 114.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 40..313
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 14..338
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 14..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g016210.1	Lcy02g016210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen