Lcy02g014550 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g014550 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 2.5e-258
Identity = 460/723 (63.62%), Postives = 560/723 (77.46%), Query Frame = 0

Query: 26  YIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEE 85
           YI+HV  PE  +  +  DLESWY SFL  T +  SS E+ R++YSY+NV+ GF+A LT+E
Sbjct: 41  YIIHVTGPEGKMLTESEDLESWYHSFLPPTLM--SSEEQPRVIYSYKNVLRGFAASLTQE 100

Query: 86  QVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITP 145
           ++ A+E+K+GFISA P+ +L+  TT+TPK+LGL +  G+WK+SNFGKGVIIGVLD+GITP
Sbjct: 101 ELSAVEKKNGFISAHPQRVLHRQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITP 160

Query: 146 AHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAI-----QSPIDD 205
            HPSF D G+P PP KWKGRC    +   + CN KLIGAR FN A+ A+     ++PID+
Sbjct: 161 GHPSFSDVGIPPPPPKWKGRC----DLNVTACNNKLIGARAFNLAAEAMNGKKAEAPIDE 220

Query: 206 DGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDA 265
           DGHGTHTASTAAGAFV  A+ LGNA+G AAGMAP AH+A+YKVC  + C  SDILAALDA
Sbjct: 221 DGHGTHTASTAAGAFVNYAEVLGNAKGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDA 280

Query: 266 AIDDGVDVLSLSLG-GRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPW 325
           A++DGVDV+S+SLG     PFF D+ AIG+FAA+QKGIFVSC+AGNSGP  S++ N APW
Sbjct: 281 AVEDGVDVISISLGLSEPPPFFNDSTAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPW 340

Query: 326 ILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQG 385
           ILTVGAS  DR IVA AKLGNG+   GES++QP  F  T LPL YAG +G + S FC  G
Sbjct: 341 ILTVGASTIDRRIVATAKLGNGQEFDGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANG 400

Query: 386 SLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATH 445
           SL +   RGKVV+CERGGGI RIAKG  VK AGGAAMI++N + + FS +A+ H LPATH
Sbjct: 401 SLDDSAFRGKVVLCERGGGIARIAKGEEVKRAGGAAMILMNDETNAFSLSADVHALPATH 460

Query: 446 VSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSRISPGILKPDIT 505
           VSY AG+ IK+YI+S+  P A+I F+GTVIG N+ +PA+ASFSSRGP+  SPGILKPDI 
Sbjct: 461 VSYAAGIEIKAYINSTATPTATILFKGTVIG-NSLAPAVASFSSRGPNLPSPGILKPDII 520

Query: 506 GPGVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAI 565
           GPGVNILAAWPFPL N+TD+K TFN+ SGTSMSCPHLSGIAAL+KS+HP+WSPAAIKSAI
Sbjct: 521 GPGVNILAAWPFPLSNSTDSKLTFNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAI 580

Query: 566 MTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-Y 625
           MTS+D  N   K IVD+ L+P + FA G+GHVNPSRA DPGLVYDIQPDDYIPYLCGL Y
Sbjct: 581 MTSADTINLGNKLIVDETLQPTDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGY 640

Query: 626 ADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGASQTSKRTVTNVGDAMSNYSLVVK 685
           ++ +V  I H+ + CS    I EG+LNYPSF+V LG+S+T  RTVTNVG+A S+Y L+V 
Sbjct: 641 SETEVGIIAHRKIKCS--ASIPEGELNYPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVA 700

Query: 686 APLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPIS 742
           AP GV V V P  LNFS VN+K TYSV+FSR  L     E+++G+L WVS KH VRSPIS
Sbjct: 701 APQGVDVKVQPYKLNFSEVNQKETYSVTFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPIS 754

BLAST of Lcy02g014550 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 4.4e-239
Identity = 441/747 (59.04%), Postives = 546/747 (73.09%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAIK---LQTYIVHVEQPELGISGDKIDLESWYTSFLSETTI 60
           M  +  F I FLL+ H   A +   LQTYIVHV++P+  +  +  DLES+Y SFL  T  
Sbjct: 1   MTCICIFSIAFLLSFHLTTAQRSTGLQTYIVHVDKPDAQVLANSADLESYYNSFLPAT-- 60

Query: 61  TTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLG 120
            + S   SR+++SY +V +GF+A+L+ E+VK ME+K GF+SA+ E +L LHTT+TP +LG
Sbjct: 61  VSGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLG 120

Query: 121 LNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASIC 180
           L +  G W++SN+GKGVIIG+LDTGITP HPSF D  MP PPAKWKG+CE     G + C
Sbjct: 121 LYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFT---GNATC 180

Query: 181 NKKLIGARTFNRASSAIQSPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAH 240
           NKK+IGAR F   S     P D++GHGTHTASTAAG FV  A   GNA G A GMAPLAH
Sbjct: 181 NKKIIGARNFISGSGV--PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAH 240

Query: 241 IAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGI 300
           IAMYKVCS  GCS +DILAALDAAIDDGVDVLSLSLGG S PF+ DNIAIG+FAAI+KGI
Sbjct: 241 IAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGI 300

Query: 301 FVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPS 360
           FVS SAGN GP  STLSN+APWILTVGAS  DR IVA A LGNG+ + GES +QP DFP 
Sbjct: 301 FVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPH 360

Query: 361 TYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMI 420
           T LPLVY G+S  + + FC  GSL   +V+GKVVVC+RGG + R+ K   VK+AGGAAMI
Sbjct: 361 TLLPLVYPGTSDEEAA-FCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMI 420

Query: 421 VINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPA 480
           + N + DG  T A+AHVLPATHV Y AG  IKSYI+S+  P A I F+GT+IG  + SP+
Sbjct: 421 LANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS-SPS 480

Query: 481 MASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLS 540
           ++SFSSRGP+  SPGI+KPDI GPGVNILAAWP  ++N T T  TFN+ISGTSMSCPHLS
Sbjct: 481 VSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLS 540

Query: 541 GIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAA 600
           GI AL+KS HP+WSPAAIKSAIMTS+D +N  G+ I+D+   PA+ FA GAGHVNPS+A+
Sbjct: 541 GIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKAS 600

Query: 601 DPGLVYDIQPDDYIPYLCGL-YADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLG-A 660
           DPGL+YDIQ +DYI YLCGL Y +  +  IV + V C   + I E +LNYPSF++ LG  
Sbjct: 601 DPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCE--SSISEAELNYPSFSIILGPK 660

Query: 661 SQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVIT 720
           +Q   RTVTNVGDA S Y++ +    GV + V P TL F+++ ++ TY+VSF++      
Sbjct: 661 TQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQS--GDF 720

Query: 721 PSEFSEGYLVWVSDKHFVRSPISVKFE 743
              F +G + W S+++ VRSPISVK E
Sbjct: 721 TDRFVQGAISWSSNEYVVRSPISVKLE 734

BLAST of Lcy02g014550 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 8.9e-232
Identity = 434/728 (59.62%), Postives = 533/728 (73.21%), Query Frame = 0

Query: 23  LQTYIVHVEQPEL-GISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSAR 82
           L TYIVHV++ E+ G      +L +W+ SFL ET      + K R+++SYRNV SGF+ R
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET------SNKDRMVFSYRNVASGFAVR 97

Query: 83  LTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDT 142
           LT E+  A++EK+  +S RPE  L+LHTT+TP +LGL +  GLW DSN GKGVIIGV+DT
Sbjct: 98  LTPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDT 157

Query: 143 GITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAIQS-PIDD 202
           GI P H SF+D+GMP PPAKWKG CE     G S+CN KLIGAR  N   SAIQ  P +D
Sbjct: 158 GIYPFHLSFNDEGMPPPPAKWKGHCEFT---GGSVCNNKLIGAR--NLVKSAIQEPPYED 217

Query: 203 DGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPK---GCSSSDILAA 262
             HGTHTA+ AAG FVEGA   GNARG AAGMAP AH+A+YKVCS K    C  S ILAA
Sbjct: 218 FFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAA 277

Query: 263 LDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDA 322
           +D AI+DGVDVLSLSLG  S+PFF D IAIG+FAA QKGIFVSCSA NSGP  S+LSN+A
Sbjct: 278 MDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEA 337

Query: 323 PWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCV 382
           PWILTVGAS  DR I A AKLGNG  + GE+L+QP+DF S  LPLVYA +     S  C 
Sbjct: 338 PWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCA 397

Query: 383 QGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPA 442
            GSL N+NV+GKVVVC+ GGGI  IAKG  V +AGG+AMI+ N +  GF+T A AHVLPA
Sbjct: 398 PGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPA 457

Query: 443 THVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSRISPGILKPD 502
            HVSY A L IK+YI+S+  P A++ F+GT+IGD + +P++A+FSSRGPS+ SPGILKPD
Sbjct: 458 VHVSYAASLAIKAYINSTYTPTATVLFQGTIIGD-SLAPSVAAFSSRGPSQQSPGILKPD 517

Query: 503 ITGPGVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKS 562
           I GPGVNILAAW   +DN       F++ISGTSMSCPHLSGIAAL+KS HP+WSPAAIKS
Sbjct: 518 IIGPGVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKS 577

Query: 563 AIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL 622
           AIMT+++  N RG  I+DQ L+PA+ FA GAGHVNP RA DPGLVYDIQP+DY+PYLCGL
Sbjct: 578 AIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGL 637

Query: 623 -YADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSL 682
            Y+D +V  IV + V C  V  I + +LNYPSF++ LG+ SQ   RT+TNVG A S Y++
Sbjct: 638 GYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTV 697

Query: 683 VVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSE-FSEGYLVWVSDKHFVR 742
            +  PL + ++V+P  + F++VN+KV Y V F   I     +  F++G + WVSDKH VR
Sbjct: 698 KIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVR 750

BLAST of Lcy02g014550 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 1.2e-231
Identity = 426/722 (59.00%), Postives = 529/722 (73.27%), Query Frame = 0

Query: 23  LQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARL 82
           L TYIVHV +P++ I  D  DL ++Y S L E+T TT+     R++++YRNV++GF+ +L
Sbjct: 42  LSTYIVHVRKPQV-IQSD--DLHTFYYSLLPESTKTTN----QRIVFTYRNVVNGFAVKL 101

Query: 83  TEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTG 142
           T E+ KA+++ +  +SARPE IL+LHTT+TP +LGL +  GLWK SN GKGVIIG+LDTG
Sbjct: 102 TPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTG 161

Query: 143 ITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAIQSPIDDDG 202
           I+P HPSF D+GMP PPAKW G CE     G   CN K+IGAR F +  + +  P DD G
Sbjct: 162 ISPFHPSFSDEGMPSPPAKWNGICEFT---GKRTCNNKIIGARNFVKTKN-LTLPFDDVG 221

Query: 203 HGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAI 262
           HGTHTASTAAG  V+GA   GNA G A GMAP AHIAMYKVC   GCS S ILA +D A+
Sbjct: 222 HGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAV 281

Query: 263 DDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILT 322
           DDGVDVLSLSLGG S PFF D IA+G+F AIQKGIFVSCSA NSGP+ S+LSN+APWILT
Sbjct: 282 DDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILT 341

Query: 323 VGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKG-SEFCVQGSL 382
           VGAS  DRTI+A AKLGNG+ + G+S++QP+DF  + LPLVYAG++G    S FC   SL
Sbjct: 342 VGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESL 401

Query: 383 SNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHVS 442
           +  +V GKVV+CE GG + R+ KG  VK+AGGAAMI++N   + F+  A+ HVLPA H+S
Sbjct: 402 NRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHIS 461

Query: 443 YTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSRISPGILKPDITGP 502
           Y AGL +K YI+S+  P A+I FEGTVIG N  +P + SFSSRGPS+ SPGILKPDI GP
Sbjct: 462 YEAGLALKEYINSTSTPTATILFEGTVIG-NLLAPQVTSFSSRGPSKASPGILKPDIIGP 521

Query: 503 GVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMT 562
           G+NILAAWP  LDN+  T   FN+ISGTSMSCPHLSGIAAL+K++HP+WSPAAIKSAIMT
Sbjct: 522 GLNILAAWPVSLDNS--TTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMT 581

Query: 563 SSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YAD 622
           ++   N  G  I+DQ L PA+ FA GAGHVNP +A DPGLVYDI+P+DYIPYLCGL Y D
Sbjct: 582 TASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTD 641

Query: 623 AQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLG-ASQTSKRTVTNVGDAMSNYSLVVKA 682
            +V  I+ + V CS V  I E +LNYPSF++ LG  +Q   RTV NVG A S Y+  +  
Sbjct: 642 REVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGV 701

Query: 683 PLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPISV 742
           P+GV ++++P  L F+ V +K+TYSVSF           F++G L WVS K+ VRSPIS 
Sbjct: 702 PVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPISF 749

BLAST of Lcy02g014550 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.5e-226
Identity = 425/731 (58.14%), Postives = 523/731 (71.55%), Query Frame = 0

Query: 21  IKLQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSA 80
           I L TYIVHV++ E   S    DL SWY SFL +T        K R+++SYR V SGF+ 
Sbjct: 33  INLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQT-----FPHKERMVFSYRKVASGFAV 92

Query: 81  RLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLD 140
           +LT E+ K+++EK   +SARPE  L LHTT+TP +LGL +  GLW D N GKGVIIG++D
Sbjct: 93  KLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIID 152

Query: 141 TGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAIQS-PID 200
           TGI P HPSF+D+GMP PPAKWKG CE     G  +CN KLIGAR  N   SAIQ  P +
Sbjct: 153 TGIFPLHPSFNDEGMPPPPAKWKGHCEFT---GGQVCNNKLIGAR--NLVKSAIQEPPFE 212

Query: 201 DDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPK-GCSSSDILAAL 260
           +  HGTHTA+ AAG F+E A   GNA+G AAGMAP AH+A+YKVC+ K GC+ S ILAA+
Sbjct: 213 NFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAM 272

Query: 261 DAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAP 320
           D AI+DGVDVLSLSLG  S+PFF D IAIG+FAA Q G+FVSCSA NSGP  STLSN+AP
Sbjct: 273 DIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAP 332

Query: 321 WILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSG----TKGSE 380
           WILTVGAS  DR IVA AKLGNGE + GE+L+QP+DF    LPLVY GS G    T+   
Sbjct: 333 WILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQS 392

Query: 381 FCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHV 440
            C+ GSL N+++ GKVV+C+  G +  I KG  V N+GG AMI+ N +  GFST A AHV
Sbjct: 393 LCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHV 452

Query: 441 LPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSRISPGIL 500
           LPA  VSY AGL IKSYI S+ NP A++ F+GT+IGD + +P++  FSSRGPS+ SPGIL
Sbjct: 453 LPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD-SLAPSVVYFSSRGPSQESPGIL 512

Query: 501 KPDITGPGVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAA 560
           KPDI GPGVNILAAW   +DN       F+++SGTSMSCPHLSGIAAL+KS+HP+WSPAA
Sbjct: 513 KPDIIGPGVNILAAWAVSVDNKI---PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAA 572

Query: 561 IKSAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYL 620
           IKSAIMT+++  N  G  I+DQ L PA+ FA GAGHVNP +A DPGLVYDI+P+DY+PYL
Sbjct: 573 IKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYL 632

Query: 621 CGL-YADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGA-SQTSKRTVTNVGDAMSN 680
           CGL Y+D ++  IV   V CS V  I E  LNYPSF++ LG+ SQ   RT+TNVG A S 
Sbjct: 633 CGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANST 692

Query: 681 YSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSE-FSEGYLVWVSDKH 740
           Y + ++ PL + ++V P  + F+ VNEKV++SV F   I     +  F +G L WVSD+H
Sbjct: 693 YKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRH 748

Query: 741 FVRSPISVKFE 743
            VR PISV F+
Sbjct: 753 AVRIPISVIFK 748

BLAST of Lcy02g014550 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 581/757 (76.75%), Postives = 653/757 (86.26%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAIK----------LQTYIVHVEQPELGISGDKIDLESWYTS 60
           MV LPF CIFF LN HG +A+K          LQTYIVHV+QP L I GD IDL++WYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTT 120
           FL E TI  SS+E+ RLLYSYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTT
Sbjct: 61  FLPE-TIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120

Query: 121 YTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEE 180
           +TP+YLGLN+ FGLWK+SNFGKGVIIGVLDTGI P HPSF+D+GM  PPAKWKGRC    
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC---- 180

Query: 181 EFGASICNKKLIGARTFNRASSAI--QSPIDDDGHGTHTASTAAGAFVEGAQALGNARGK 240
           EFGASICN KLIGARTFN A++ +  +SP D++GHGTHTASTAAG FV+GA+ALGNARGK
Sbjct: 181 EFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGK 240

Query: 241 AAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIG 300
           A GMAPLAHIAMYKVCSP GCSSSDILAALDAAIDDGVDVLSLSLG  S PFF D IA+G
Sbjct: 241 AVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMG 300

Query: 301 SFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGES 360
           +FAAI+KGIFVSCSAGNSGPS++TL+N+APWILTVGAS  DR IVALAKLG+GEV+ GES
Sbjct: 301 AFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGES 360

Query: 361 LYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVV 420
           L+QPR+FPS +LPLVYAG SG +GSE+C+QGSL  +NV GKVVVCERGGGI R+AKG+VV
Sbjct: 361 LFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVV 420

Query: 421 KNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTV 480
           KN GGAAMI+INQ+P+GFST AEAHVLP TH+SY  GL+IK+YI+SS NP ASISF+GT+
Sbjct: 421 KNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL 480

Query: 481 IGD--NNFSPAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDN--NTDTKSTFN 540
           +G+    FSPAMASFSSRGP + SPGILKPDITGPGVNILAAWPFPL+N  NT+TKSTFN
Sbjct: 481 LGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFN 540

Query: 541 VISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANFF 600
           VISGTSMSCPHLSGIAAL+KS HPNWSPAAIKSAIMTS+D+ NP+GK I+DQDLKPANFF
Sbjct: 541 VISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFF 600

Query: 601 AMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGDL 660
           AMG+GHVNPS+AA+PGLVYDIQPDDYIPYLC LY DAQV+ IV K VTCSTV RIREGDL
Sbjct: 601 AMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDL 660

Query: 661 NYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTY 720
           NYPSFAV+LGA SQT  RTVTNVGDA S Y  +V+AP GVSV VTP  L FS++NEKVTY
Sbjct: 661 NYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTY 720

Query: 721 SVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPISVK 741
           SV+FSR   V T SEFSEGYL+WVS K  VRSPISVK
Sbjct: 721 SVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752

BLAST of Lcy02g014550 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 568/725 (78.34%), Postives = 638/725 (88.00%), Query Frame = 0

Query: 23  LQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARL 82
           LQTYIVHV+QPE+ I GD IDL++WYTSFL E TI  SSNE+SRLLYSYR+V+SGFSARL
Sbjct: 13  LQTYIVHVKQPEVEILGDTIDLQNWYTSFLPE-TIEASSNEQSRLLYSYRHVISGFSARL 72

Query: 83  TEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTG 142
           T+EQVK MEEKDGFISA PE+ LNLHTT+TP+YLGLN+ FGLWK+SNFGKGVIIGVLDTG
Sbjct: 73  TKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTG 132

Query: 143 ITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRAS--SAIQSPIDD 202
           I P HPSF+D+GMP PPAKWKGRC    EFGASICN KLIGARTFN A+  S  +SP D+
Sbjct: 133 IHPNHPSFNDEGMPSPPAKWKGRC----EFGASICNNKLIGARTFNLANNVSIGKSPNDE 192

Query: 203 DGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDA 262
           +GHGTHTASTAAG FV+GA+ALGNARGKA GMAPLAHIA+YKVCSPKGCSSSDILAALDA
Sbjct: 193 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDA 252

Query: 263 AIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWI 322
           AIDDGVDVLSLSLG  S PFF D IA+G+FAAI+KGIFVSCSAGNSGPS++TL+N+APWI
Sbjct: 253 AIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWI 312

Query: 323 LTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGS 382
           LTVGAS  DR IVALAKL +G+V  GESL+QPRDF S +LPLVYAG SG +GSE+CV+GS
Sbjct: 313 LTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGS 372

Query: 383 LSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHV 442
           L  +NV GK+VVCERGGGIGRIAKG+VVKN GGAAMI++NQ+PDGFST AEAHVLP TH+
Sbjct: 373 LEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHL 432

Query: 443 SYTAGLRIKSYISSSQNPIASISFEGTVIGD--NNFSPAMASFSSRGPSRISPGILKPDI 502
           SY  GL+IK YI+SS NP ASISFEGT++G+    FSPAMASFSSRGP + SPGILKPDI
Sbjct: 433 SYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDI 492

Query: 503 TGPGVNILAAWPFPLDN--NTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIK 562
           TGPGVNILAAWPFPL+N  NT+TKSTFNVISGTSMSCPHLSGIAAL+KS HPNWSPAAIK
Sbjct: 493 TGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIK 552

Query: 563 SAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCG 622
           SAIMTS+D+ NP+GK IVDQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQPDDY+PYLC 
Sbjct: 553 SAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCH 612

Query: 623 LYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSL 682
           LY DAQV+ IV + VTCSTV+RIREGDLNYPSFAV+LGA SQ   RTVTNVGDA S Y  
Sbjct: 613 LYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYA 672

Query: 683 VVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRS 741
           +VKAP GVSV VTPR L FS++NEK+TYSV+FSR   V T SEFSEGYL+WVS+KH VRS
Sbjct: 673 IVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRS 732

BLAST of Lcy02g014550 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 566/725 (78.07%), Postives = 634/725 (87.45%), Query Frame = 0

Query: 23  LQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARL 82
           LQTYIVHV+QP L I GD IDL++WYTSFL E TI  SS+E+ RLLYSYR+VMSGFSARL
Sbjct: 13  LQTYIVHVKQPVLEILGDTIDLQNWYTSFLPE-TIEASSDEQPRLLYSYRHVMSGFSARL 72

Query: 83  TEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTG 142
           T+EQVKAMEEKDGFISA PE+ LNLHTT+TP+YLGLN  FGLWK+SNFGKGVIIGVLDTG
Sbjct: 73  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTG 132

Query: 143 ITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAI--QSPIDD 202
           I P HPSF+D+GM  PPAKWKGRC    EFGASICN KLIGARTFN A++ +  +SP D+
Sbjct: 133 IHPNHPSFNDEGMSSPPAKWKGRC----EFGASICNNKLIGARTFNLANNFVIGKSPNDE 192

Query: 203 DGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDA 262
           +GHGTHTASTAAG FV+GA+ALGNARGKA GMAPLAHIAMYKVCSP GCSSSDILAALDA
Sbjct: 193 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDA 252

Query: 263 AIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWI 322
           AIDDGVDVLSLSLG  S PFF D IAIG+FAAI+KGIFVSCSAGNSGPS++TL+N+APWI
Sbjct: 253 AIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWI 312

Query: 323 LTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGS 382
           LTVGAS  DR IVALAKLG+GEV+ GESL+QPR+FPS +LPLVYAG SG +GSE+C+QGS
Sbjct: 313 LTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGS 372

Query: 383 LSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHV 442
           L  +NV GKVVVCERGGGI R+AKG+VVKN GGAAMI+INQ+P+GFST AEAHVLP TH+
Sbjct: 373 LEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHL 432

Query: 443 SYTAGLRIKSYISSSQNPIASISFEGTVIGD--NNFSPAMASFSSRGPSRISPGILKPDI 502
           SY  GL+IK+YI+SS NP ASISF+GT++G+    FSPAMASFSSRGP + SPGILKPDI
Sbjct: 433 SYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDI 492

Query: 503 TGPGVNILAAWPFPLDN--NTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIK 562
           TGPGVNILAAWPFPL+N  NT+TKSTFNVISGTSMSCPHLSGIAAL+KS HPNWSPAAIK
Sbjct: 493 TGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIK 552

Query: 563 SAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCG 622
           SAIMTS+D+ NP+GK I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQPDDYIPYLC 
Sbjct: 553 SAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCH 612

Query: 623 LYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSL 682
           LY DAQV+ IV K VTCSTV RIREGDLNYPSFAV+L A SQT  RTVTNVGDA S Y  
Sbjct: 613 LYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYP 672

Query: 683 VVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRS 741
           +V+AP GVSV VTP  L FS++NEKVTYSV+FSR   V T SEFSEGYL+WVS K  VRS
Sbjct: 673 IVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRS 732

BLAST of Lcy02g014550 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.7e-302
Identity = 541/760 (71.18%), Postives = 634/760 (83.42%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAI-----------KLQTYIVHVEQPELGISGDKID--LESW 60
           MV+L F  IFFLLN+  ++A            KLQTYIVHVEQP++ + G+  +  +ESW
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNL 120
           Y SF+ ++T TT   E+ +LLYSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++L
Sbjct: 61  YMSFIPKSTETTV--EQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120

Query: 121 HTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCE 180
           HTT+TP+YLGLN+QFG+WK+SNFGKGVI+GVLDTGI P HPSF+D+GM  PPAKWKG+C 
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKC- 180

Query: 181 EEEEFGASICNKKLIGARTFNRASSAI--QSPIDDDGHGTHTASTAAGAFVEGAQALGNA 240
              EF +S+CN KLIGARTFN  ++ +  +SP D+ GHGTHTASTAAGAFVE A+ALGNA
Sbjct: 181 ---EFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNA 240

Query: 241 RGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNI 300
           +GKAAG+APLAH+A+YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG RS+PFF DNI
Sbjct: 241 KGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNI 300

Query: 301 AIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHF 360
           A+ +F AIQKGIFVS SAGNSGP  STLSNDAPWILTVGAS  +R IVA+AKLGNGE + 
Sbjct: 301 AVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYE 360

Query: 361 GESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKG 420
           GESLYQP DFPS +LPLVYAG+   K   FC +GSL NM+V+GKVVVCE  GG+GR+AKG
Sbjct: 361 GESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKG 420

Query: 421 MVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFE 480
           +VVKNAGGAAMI+INQ+ DGFST +EAHVLPATHVSY AG+ IKSYI+SSQNP ASISF+
Sbjct: 421 LVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFK 480

Query: 481 GTVIGD-NNFS-PAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDNNTDTKSTF 540
           GTVIGD ++FS P+MASFSSRGP   SPGILKPDITGPGVNILAAWPFPLDN+T+TKSTF
Sbjct: 481 GTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTF 540

Query: 541 NVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANF 600
           NVISGTSMSCPHLSGIAAL+KS HPNWSPAAIKSAIMT+++I  P+G+ IVDQDL+PANF
Sbjct: 541 NVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANF 600

Query: 601 FAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGD 660
           FAMGAGHVNPS+AADPGLVYDIQPDDYIPYLCGLY D +V+ IVH+ V C  V  IREGD
Sbjct: 601 FAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGD 660

Query: 661 LNYPSFAVTLGASQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTY 720
           LNYPSF+V LG  QT KRTVTNVG+A S Y+ +V+APLGVS+TV PR L FSRVN+ +T+
Sbjct: 661 LNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTF 720

Query: 721 SVSFSRDILVITPSEFSEGYLVWV--SDKHFVRSPISVKF 742
           +V+F+R   V    EF EGYL WV  S K+ VRSP+SVKF
Sbjct: 721 TVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754

BLAST of Lcy02g014550 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 3.6e-300
Identity = 535/751 (71.24%), Postives = 627/751 (83.49%), Query Frame = 0

Query: 10  FFLLNVHGNIAI-----------KLQTYIVHVEQPELGISGDKID--LESWYTSFLSETT 69
           FFLLN+  ++A            KLQTYIVHVEQP++ + G+  +  +ESWY SF+ ++T
Sbjct: 19  FFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKST 78

Query: 70  ITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYL 129
            TT   E+ +LLYSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++LHTT+TP+YL
Sbjct: 79  ETTV--EQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYL 138

Query: 130 GLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASI 189
           GLN+QFG+WK+SNFGKGVI+GVLDTGI P HPSF+D+GM  PPAKWKG+C    EF +S+
Sbjct: 139 GLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKC----EFNSSL 198

Query: 190 CNKKLIGARTFNRASSAI--QSPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAP 249
           CN KLIGARTFN  ++ +  +SP D+ GHGTHTASTAAGAFVE A+ALGNA+GKAAG+AP
Sbjct: 199 CNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAP 258

Query: 250 LAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQ 309
           LAH+A+YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG RS+PFF DNIA+ +F AIQ
Sbjct: 259 LAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQ 318

Query: 310 KGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRD 369
           KGIFVS SAGNSGP  STLSNDAPWILTVGAS  +R IVA+AKLGNGE + GESLYQP D
Sbjct: 319 KGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPND 378

Query: 370 FPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGA 429
           FPS +LPLVYAG+   K   FC +GSL NM+V+GKVVVCE  GG+GR+AKG+VVKNAGGA
Sbjct: 379 FPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGA 438

Query: 430 AMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGD-NN 489
           AMI+INQ+ DGFST +EAHVLPATHVSY AG+ IKSYI+SSQNP ASISF+GTVIGD ++
Sbjct: 439 AMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDD 498

Query: 490 FS-PAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDNNTDTKSTFNVISGTSMS 549
           FS P+MASFSSRGP   SPGILKPDITGPGVNILAAWPFPLDN+T+TKSTFNVISGTSMS
Sbjct: 499 FSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTSMS 558

Query: 550 CPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVN 609
           CPHLSGIAAL+KS HPNWSPAAIKSAIMT+++I  P+G+ I DQDL+PANFFAMGAGHVN
Sbjct: 559 CPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVN 618

Query: 610 PSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVT 669
           PS+AADPGLVYDIQPDDYIPYLCGLY D +V+ IVH+ V C  V  IREGDLNYPSF+V 
Sbjct: 619 PSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVA 678

Query: 670 LGASQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDIL 729
           LG  QT KRTVTNVG+A S Y+ +V+APLGVS+TV PR L FSRVN+ +T++V+F+R   
Sbjct: 679 LGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRW 738

Query: 730 VITPSEFSEGYLVWV--SDKHFVRSPISVKF 742
           V    EF EGYL WV  S K+ VRSP+SVKF
Sbjct: 739 VKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 763

BLAST of Lcy02g014550 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 597/726 (82.23%), Postives = 657/726 (90.50%), Query Frame = 0

Query: 21  IKLQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSA 80
           IKLQTYIVHV++ EL I GD IDLESWYTSFL ET  T  SNE+SRLLYS+RNVMSGFSA
Sbjct: 12  IKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKT--SNEQSRLLYSFRNVMSGFSA 71

Query: 81  RLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLD 140
           RLTEE VKAMEEK+GFISARPE+ILNLHTT+TP+YLGLN+QFGLWKDSNFGKGVIIGVLD
Sbjct: 72  RLTEE-VKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLD 131

Query: 141 TGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAI--QSPI 200
           TGI+P HPSF+DDGMP PPAKWKGRC    EFGASICN KLIGARTFNRA+SA+  +SPI
Sbjct: 132 TGISPDHPSFNDDGMPQPPAKWKGRC----EFGASICNNKLIGARTFNRANSALMGESPI 191

Query: 201 DDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAAL 260
           D++GHGTHTASTAAG FVEGA+ALGNARGKA GMAPLAH+AMYKVCSPKGCSSSDILAAL
Sbjct: 192 DENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDILAAL 251

Query: 261 DAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAP 320
           DAAI DGVDVLSLSLG  S PF++DNIAIG+FAAI+ GIFVSCSAGNSGPS+STL+N+AP
Sbjct: 252 DAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAP 311

Query: 321 WILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQ 380
           WILTVGAS  DRTI+ALA+LGNGEVH GESL+QPRDFPS++LPLVYAGSSG KGSEFCVQ
Sbjct: 312 WILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSEFCVQ 371

Query: 381 GSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPAT 440
           GSL+N+NV+GK+VVCERGGGIGRIAKGMVVKNAGGAAMI+INQ+ D FST AEAH+LPAT
Sbjct: 372 GSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHILPAT 431

Query: 441 HVSYTAGLRIKSYISSSQNPIASISFEGTVIGD--NNFSPAMASFSSRGPSRISPGILKP 500
           HVSY AGL+IKSYI SSQNP+ASISFEGT+IG     FSPAMASFSSRGP   SPGILKP
Sbjct: 432 HVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPGILKP 491

Query: 501 DITGPGVNILAAWPFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIK 560
           DITGPGVNILAAWPF LDNNT+ KSTFNVISGTSMSCPHLSGIAAL+KS HPNWSPAAIK
Sbjct: 492 DITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIK 551

Query: 561 SAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCG 620
           SAIMTS+D+ N +G+ IVDQDLKPANFFAMG+GHVNPS+AADPGLVYDIQPDDYIPYLCG
Sbjct: 552 SAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCG 611

Query: 621 LYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGASQTSKRTVTNVGDAMSNYSLV 680
           LY+DAQV+ IV + VTCST++RIREGDLNYPSFAVTLG SQ   RTVTNVGDA S YS +
Sbjct: 612 LYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSIYSAI 671

Query: 681 VKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRSP 740
           V+AP+GVSV VTP  L FSRV EKVTYSV+FSRD LV T SEFSEGYL+WVS+KH VRSP
Sbjct: 672 VEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHMVRSP 730

Query: 741 ISVKFE 743
           ISVK +
Sbjct: 732 ISVKLK 730

BLAST of Lcy02g014550 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 583/757 (77.01%), Postives = 655/757 (86.53%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAIK----------LQTYIVHVEQPELGISGDKIDLESWYTS 60
           MV LPF CIFF LN HG +A+K          LQTYIVHV+QPE+ I GD IDL++WYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTS 60

Query: 61  FLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTT 120
           FL E TI  SSNE+SRLLYSYR+V+SGFSARLT+EQVK MEEKDGFISA PE+ LNLHTT
Sbjct: 61  FLPE-TIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTT 120

Query: 121 YTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEE 180
           +TP+YLGLN+ FGLWK+SNFGKGVIIGVLDTGI P HPSF+D+GMP PPAKWKGRC    
Sbjct: 121 HTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRC---- 180

Query: 181 EFGASICNKKLIGARTFNRAS--SAIQSPIDDDGHGTHTASTAAGAFVEGAQALGNARGK 240
           EFGASICN KLIGARTFN A+  S  +SP D++GHGTHTASTAAG FV+GA+ALGNARGK
Sbjct: 181 EFGASICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGK 240

Query: 241 AAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIG 300
           A GMAPLAHIA+YKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLG  S PFF D IA+G
Sbjct: 241 AVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVG 300

Query: 301 SFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGES 360
           +FAAI+KGIFVSCSAGNSGPS++TL+N+APWILTVGAS  DR IVALAKL +G+V  GES
Sbjct: 301 AFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGES 360

Query: 361 LYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVV 420
           L+QPRDF S +LPLVYAG SG +GSE+CV+GSL  +NV GK+VVCERGGGIGRIAKG+VV
Sbjct: 361 LFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVV 420

Query: 421 KNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTV 480
           KN GGAAMI++NQ+PDGFST AEAHVLP TH+SY  GL+IK YI+SS NP ASISFEGT+
Sbjct: 421 KNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTL 480

Query: 481 IGD--NNFSPAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDN--NTDTKSTFN 540
           +G+    FSPAMASFSSRGP + SPGILKPDITGPGVNILAAWPFPL+N  NT+TKSTFN
Sbjct: 481 LGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFN 540

Query: 541 VISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANFF 600
           VISGTSMSCPHLSGIAAL+KS HPNWSPAAIKSAIMTS+D+ NP+GK IVDQDLKPANFF
Sbjct: 541 VISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFF 600

Query: 601 AMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGDL 660
           AMG+GHVNPS+AA+PGLVYDIQPDDY+PYLC LY DAQV+ IV + VTCSTV+RIREGDL
Sbjct: 601 AMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDL 660

Query: 661 NYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTY 720
           NYPSFAV+LGA SQ   RTVTNVGDA S Y  +VKAP GVSV VTPR L FS++NEK+TY
Sbjct: 661 NYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTY 720

Query: 721 SVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPISVK 741
           SV+FSR   V T SEFSEGYL+WVS+KH VRSPISVK
Sbjct: 721 SVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVK 752

BLAST of Lcy02g014550 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 581/757 (76.75%), Postives = 653/757 (86.26%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAIK----------LQTYIVHVEQPELGISGDKIDLESWYTS 60
           MV LPF CIFF LN HG +A+K          LQTYIVHV+QP L I GD IDL++WYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTT 120
           FL E TI  SS+E+ RLLYSYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTT
Sbjct: 61  FLPE-TIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120

Query: 121 YTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEE 180
           +TP+YLGLN+ FGLWK+SNFGKGVIIGVLDTGI P HPSF+D+GM  PPAKWKGRC    
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC---- 180

Query: 181 EFGASICNKKLIGARTFNRASSAI--QSPIDDDGHGTHTASTAAGAFVEGAQALGNARGK 240
           EFGASICN KLIGARTFN A++ +  +SP D++GHGTHTASTAAG FV+GA+ALGNARGK
Sbjct: 181 EFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGK 240

Query: 241 AAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIG 300
           A GMAPLAHIAMYKVCSP GCSSSDILAALDAAIDDGVDVLSLSLG  S PFF D IA+G
Sbjct: 241 AVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMG 300

Query: 301 SFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGES 360
           +FAAI+KGIFVSCSAGNSGPS++TL+N+APWILTVGAS  DR IVALAKLG+GEV+ GES
Sbjct: 301 AFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGES 360

Query: 361 LYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVV 420
           L+QPR+FPS +LPLVYAG SG +GSE+C+QGSL  +NV GKVVVCERGGGI R+AKG+VV
Sbjct: 361 LFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVV 420

Query: 421 KNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTV 480
           KN GGAAMI+INQ+P+GFST AEAHVLP TH+SY  GL+IK+YI+SS NP ASISF+GT+
Sbjct: 421 KNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL 480

Query: 481 IGD--NNFSPAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDN--NTDTKSTFN 540
           +G+    FSPAMASFSSRGP + SPGILKPDITGPGVNILAAWPFPL+N  NT+TKSTFN
Sbjct: 481 LGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFN 540

Query: 541 VISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANFF 600
           VISGTSMSCPHLSGIAAL+KS HPNWSPAAIKSAIMTS+D+ NP+GK I+DQDLKPANFF
Sbjct: 541 VISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFF 600

Query: 601 AMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGDL 660
           AMG+GHVNPS+AA+PGLVYDIQPDDYIPYLC LY DAQV+ IV K VTCSTV RIREGDL
Sbjct: 601 AMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDL 660

Query: 661 NYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTY 720
           NYPSFAV+LGA SQT  RTVTNVGDA S Y  +V+AP GVSV VTP  L FS++NEKVTY
Sbjct: 661 NYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTY 720

Query: 721 SVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPISVK 741
           SV+FSR   V T SEFSEGYL+WVS K  VRSPISVK
Sbjct: 721 SVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752

BLAST of Lcy02g014550 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 566/725 (78.07%), Postives = 634/725 (87.45%), Query Frame = 0

Query: 23  LQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARL 82
           LQTYIVHV+QP L I GD IDL++WYTSFL E TI  SS+E+ RLLYSYR+VMSGFSARL
Sbjct: 13  LQTYIVHVKQPVLEILGDTIDLQNWYTSFLPE-TIEASSDEQPRLLYSYRHVMSGFSARL 72

Query: 83  TEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTG 142
           T+EQVKAMEEKDGFISA PE+ LNLHTT+TP+YLGLN  FGLWK+SNFGKGVIIGVLDTG
Sbjct: 73  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTG 132

Query: 143 ITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAI--QSPIDD 202
           I P HPSF+D+GM  PPAKWKGRC    EFGASICN KLIGARTFN A++ +  +SP D+
Sbjct: 133 IHPNHPSFNDEGMSSPPAKWKGRC----EFGASICNNKLIGARTFNLANNFVIGKSPNDE 192

Query: 203 DGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDA 262
           +GHGTHTASTAAG FV+GA+ALGNARGKA GMAPLAHIAMYKVCSP GCSSSDILAALDA
Sbjct: 193 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDA 252

Query: 263 AIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWI 322
           AIDDGVDVLSLSLG  S PFF D IAIG+FAAI+KGIFVSCSAGNSGPS++TL+N+APWI
Sbjct: 253 AIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWI 312

Query: 323 LTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGS 382
           LTVGAS  DR IVALAKLG+GEV+ GESL+QPR+FPS +LPLVYAG SG +GSE+C+QGS
Sbjct: 313 LTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGS 372

Query: 383 LSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHV 442
           L  +NV GKVVVCERGGGI R+AKG+VVKN GGAAMI+INQ+P+GFST AEAHVLP TH+
Sbjct: 373 LEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHL 432

Query: 443 SYTAGLRIKSYISSSQNPIASISFEGTVIGD--NNFSPAMASFSSRGPSRISPGILKPDI 502
           SY  GL+IK+YI+SS NP ASISF+GT++G+    FSPAMASFSSRGP + SPGILKPDI
Sbjct: 433 SYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDI 492

Query: 503 TGPGVNILAAWPFPLDN--NTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIK 562
           TGPGVNILAAWPFPL+N  NT+TKSTFNVISGTSMSCPHLSGIAAL+KS HPNWSPAAIK
Sbjct: 493 TGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIK 552

Query: 563 SAIMTSSDITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCG 622
           SAIMTS+D+ NP+GK I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQPDDYIPYLC 
Sbjct: 553 SAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCH 612

Query: 623 LYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGA-SQTSKRTVTNVGDAMSNYSL 682
           LY DAQV+ IV K VTCSTV RIREGDLNYPSFAV+L A SQT  RTVTNVGDA S Y  
Sbjct: 613 LYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYP 672

Query: 683 VVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRS 741
           +V+AP GVSV VTP  L FS++NEKVTYSV+FSR   V T SEFSEGYL+WVS K  VRS
Sbjct: 673 IVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRS 732

BLAST of Lcy02g014550 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1048.1 bits (2709), Expect = 3.5e-302
Identity = 541/760 (71.18%), Postives = 634/760 (83.42%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAI-----------KLQTYIVHVEQPELGISGDKID--LESW 60
           MV+L F  IFFLLN+  ++A            KLQTYIVHVEQP++ + G+  +  +ESW
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNL 120
           Y SF+ ++T TT   E+ +LLYSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++L
Sbjct: 61  YMSFIPKSTETTV--EQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120

Query: 121 HTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCE 180
           HTT+TP+YLGLN+QFG+WK+SNFGKGVI+GVLDTGI P HPSF+D+GM  PPAKWKG+C 
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKC- 180

Query: 181 EEEEFGASICNKKLIGARTFNRASSAI--QSPIDDDGHGTHTASTAAGAFVEGAQALGNA 240
              EF +S+CN KLIGARTFN  ++ +  +SP D+ GHGTHTASTAAGAFVE A+ALGNA
Sbjct: 181 ---EFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNA 240

Query: 241 RGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNI 300
           +GKAAG+APLAH+A+YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG RS+PFF DNI
Sbjct: 241 KGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNI 300

Query: 301 AIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHF 360
           A+ +F AIQKGIFVS SAGNSGP  STLSNDAPWILTVGAS  +R IVA+AKLGNGE + 
Sbjct: 301 AVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYE 360

Query: 361 GESLYQPRDFPSTYLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKG 420
           GESLYQP DFPS +LPLVYAG+   K   FC +GSL NM+V+GKVVVCE  GG+GR+AKG
Sbjct: 361 GESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKG 420

Query: 421 MVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFE 480
           +VVKNAGGAAMI+INQ+ DGFST +EAHVLPATHVSY AG+ IKSYI+SSQNP ASISF+
Sbjct: 421 LVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFK 480

Query: 481 GTVIGD-NNFS-PAMASFSSRGPSRISPGILKPDITGPGVNILAAWPFPLDNNTDTKSTF 540
           GTVIGD ++FS P+MASFSSRGP   SPGILKPDITGPGVNILAAWPFPLDN+T+TKSTF
Sbjct: 481 GTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTF 540

Query: 541 NVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDLKPANF 600
           NVISGTSMSCPHLSGIAAL+KS HPNWSPAAIKSAIMT+++I  P+G+ IVDQDL+PANF
Sbjct: 541 NVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANF 600

Query: 601 FAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGLYADAQVATIVHKLVTCSTVTRIREGD 660
           FAMGAGHVNPS+AADPGLVYDIQPDDYIPYLCGLY D +V+ IVH+ V C  V  IREGD
Sbjct: 601 FAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGD 660

Query: 661 LNYPSFAVTLGASQTSKRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTY 720
           LNYPSF+V LG  QT KRTVTNVG+A S Y+ +V+APLGVS+TV PR L FSRVN+ +T+
Sbjct: 661 LNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTF 720

Query: 721 SVSFSRDILVITPSEFSEGYLVWV--SDKHFVRSPISVKF 742
           +V+F+R   V    EF EGYL WV  S K+ VRSP+SVKF
Sbjct: 721 TVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754

BLAST of Lcy02g014550 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 629.8 bits (1623), Expect = 2.8e-180
Identity = 368/776 (47.42%), Postives = 488/776 (62.89%), Query Frame = 0

Query: 5   PFF-CIFFLLNVHGNIAI-KLQTYIV--HVEQPELGISGDKIDLESWYTSFLSETTITTS 64
           PFF CI FLL    +  I + QTYIV  H           K D   W+ SFL E  +   
Sbjct: 5   PFFLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFD---WHLSFLQEAVLGVE 64

Query: 65  SNEK---SRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLG 124
             E+   SRLLYSY + + GF+A+LTE + + +      ++ RP+ +L + TTY+ K+LG
Sbjct: 65  EEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLG 124

Query: 125 LN--EQFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGAS 184
           L+     G+W  S FG+G IIGVLDTG+ P  PSF D GMP  P KWKG C+E E F +S
Sbjct: 125 LDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSS 184

Query: 185 ICNKKLIGARTFNRASSAIQSP-------------IDDDGHGTHTASTAAGAFVEGAQAL 244
            CN+KLIGAR F R      SP              D  GHGTHTAST  G+ V  A  L
Sbjct: 185 SCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVL 244

Query: 245 GNARGKAAGMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFL 304
           GN  G A GMAP AHIA+YKVC   GC SSDILAA+D AI D VDVLSLSLGG  IP + 
Sbjct: 245 GNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYD 304

Query: 305 DNIAIGSFAAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGE 364
           D IAIG+F A+++GI V C+AGN+GP ES+++N APW+ T+GA   DR   A+ +L NG+
Sbjct: 305 DTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGK 364

Query: 365 VHFGESLYQPRDFPST--YLPLVYAGSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIG 424
           + +GESLY  +   +    + ++Y  + G KGSEFC++GSL    +RGK+V+C+RG   G
Sbjct: 365 LLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-G 424

Query: 425 RIAKGMVVKNAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIA 484
           R  KG  VK AGG AMI+ N + +    + + H+LPAT + YT  + +K+Y++++  P A
Sbjct: 425 RSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKA 484

Query: 485 SISFEGTVIGDNNFSPAMASFSSRGPSRISPGILKPDITGPGVNILAAWP-------FPL 544
            I F GTVIG +  +P +A FS+RGPS  +P ILKPD+  PGVNI+AAWP        P 
Sbjct: 485 RIIFGGTVIGRSR-APEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPY 544

Query: 545 DNNTDTKSTFNVISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSI 604
           D+    +  F V+SGTSMSCPH+SGI AL++S +PNWSPAAIKSA+MT++D+ + +GK+I
Sbjct: 545 DSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAI 604

Query: 605 VDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YADAQVATIVHKLVT 664
            D + KPA  FA+GAGHVNP +A +PGLVY+IQP DYI YLC L +  + +  I HK V+
Sbjct: 605 KDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVS 664

Query: 665 CSTVTRIREG-DLNYPSFAVTLGASQTSK---RTVTNVGDAMSNYSLVVKAPLGVSVTVT 724
           C+ + R   G  LNYPS AV     +T++   R VTNVG   S YS+ VKAP G+ V V 
Sbjct: 665 CNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVN 724

Query: 725 PRTLNFSRVNEKVTYSVSF--SRDILVITPSEFSEGYLVWVSDKHF---VRSPISV 740
           P+ L F  V++ ++Y V F   +       + F++G L WV+  +    VRSPISV
Sbjct: 725 PKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISV 770

BLAST of Lcy02g014550 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 601.7 bits (1550), Expect = 8.2e-172
Identity = 351/738 (47.56%), Postives = 462/738 (62.60%), Query Frame = 0

Query: 25  TYIVHVEQPELGISGDKIDLES-WYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSARLT 84
           TYIVH+ + ++  S    DL S WY S L       S ++ + LLY+Y N + GFS RLT
Sbjct: 31  TYIVHMAKSQMPSS---FDLHSNWYDSSL------RSISDSAELLYTYENAIHGFSTRLT 90

Query: 85  EEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFG-LWKDSNFGKGVIIGVLDTG 144
           +E+  ++  + G IS  PE    LHTT TP +LGL+E    L+ ++     V++GVLDTG
Sbjct: 91  QEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTG 150

Query: 145 ITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRA----------SS 204
           + P   S+ D+G    P+ WKG CE    F AS+CN+KLIGAR F R           S 
Sbjct: 151 VWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESK 210

Query: 205 AIQSPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKGCSSS 264
             +SP DDDGHGTHT+STAAG+ VEGA  LG A G A GMAP A +A+YKVC   GC SS
Sbjct: 211 ESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSS 270

Query: 265 DILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGPSEST 324
           DILAA+D AI D V+VLS+SLGG    ++ D +AIG+FAA+++GI VSCSAGN+GPS S+
Sbjct: 271 DILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSS 330

Query: 325 LSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAG-SSGTK 384
           LSN APWI TVGA   DR   ALA LGNG+   G SL++    P   LP +YAG +S   
Sbjct: 331 LSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT 390

Query: 385 GSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFSTAAE 444
               C+ G+L    V+GK+V+C+RG    R+ KG VVK AGG  MI+ N   +G    A+
Sbjct: 391 NGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAANGEELVAD 450

Query: 445 AHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSRISP 504
           AH+LPAT V   AG  I+ Y+++  NP ASIS  GTV+G    SP +A+FSSRGP+ I+P
Sbjct: 451 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP-SPVVAAFSSRGPNSITP 510

Query: 505 GILKPDITGPGVNILAAW---PFPLDNNTDTKST-FNVISGTSMSCPHLSGIAALVKSTH 564
            ILKPD+  PGVNILAAW     P    +D++   FN+ISGTSMSCPH+SG+AAL+KS H
Sbjct: 511 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 570

Query: 565 PNWSPAAIKSAIMTSSDITNPRGKSIVD-QDLKPANFFAMGAGHVNPSRAADPGLVYDIQ 624
           P WSPAAI+SA+MT++  T   GK ++D    KP+  F  GAGHV+P+ A +PGL+YD+ 
Sbjct: 571 PEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLT 630

Query: 625 PDDYIPYLCGL-YADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGASQTSK--RTV 684
            +DY+ +LC L Y   Q+ ++  +  TC         DLNYPSFAV +      K  RTV
Sbjct: 631 TEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 690

Query: 685 TNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSRDILVITPSEFSEGY 742
           T+VG A +    V     GV ++V P  LNF   NEK +Y+V+F+ D    + S  S G 
Sbjct: 691 TSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGS 750

BLAST of Lcy02g014550 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 599.7 bits (1545), Expect = 3.1e-171
Identity = 348/768 (45.31%), Postives = 474/768 (61.72%), Query Frame = 0

Query: 1   MVILPFFCIFFLLNVHGNIAIKLQTYIVHVEQPELGISGDKIDLESWYTSFLSETTITTS 60
           M    F   FFL+    N++ + QTY++H        +  K  + S + S  +E  I   
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIHTV-----TTSTKHIVTSLFNSLQTE-NINDD 78

Query: 61  SNEKSRLLYSYRNVMSGFSARLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNE 120
                 + Y Y N MSGFSA LT++Q+  ++   GFISA P+ +L+LHTTY+ ++LGL  
Sbjct: 79  DFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEF 138

Query: 121 QFGLWKDSNFGKGVIIGVLDTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKK 180
             GLW +++    VIIG++DTGI+P H SF D  M   P++W+G C+E   F +S CNKK
Sbjct: 139 GIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKK 198

Query: 181 LIGARTFNRASSAI----------QSPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAA 240
           +IGA  F +   +I          +S  D  GHGTHTASTAAG  V  A   G A+G A+
Sbjct: 199 IIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLAS 258

Query: 241 GMAPLAHIAMYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSF 300
           GM   + IA YK C   GC+S+D++AA+D AI DGVDV+SLSLGG S PF++D IAI  F
Sbjct: 259 GMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGF 318

Query: 301 AAIQKGIFVSCSAGNSGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLY 360
            A+QK IFVSCSAGNSGP+ ST+SN APW++TV AS TDRT  A+ ++GN +   G SLY
Sbjct: 319 GAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLY 378

Query: 361 QPRDFPSTYLPLVYAGSSGTK-GSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVK 420
           + +   +  LPL +  ++G + G+ FC++ SL    V GK+V+C RG   GR AKG  VK
Sbjct: 379 KGKSLKN--LPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVK 438

Query: 421 NAGGAAMIVINQQPDGFSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVI 480
            +GGAAM++++ + +G    A+ HVLPA  + ++ G  + +Y++ + N  AS+ F GT  
Sbjct: 439 RSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAY 498

Query: 481 GDNNFSPAMASFSSRGPSRISPGILKPDITGPGVNILAAW-PF---PLDNNTDTKSTFNV 540
           G    +P +A+FSSRGPS   P I KPDI  PG+NILA W PF    L  +   +  FN+
Sbjct: 499 GAT--APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNI 558

Query: 541 ISGTSMSCPHLSGIAALVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDL----KPA 600
           ISGTSM+CPH+SGIAAL+KS H +WSPA IKSAIMT++ IT+ R + I D+        A
Sbjct: 559 ISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAA 618

Query: 601 NFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YADAQVATIVHKLVTC-STVTRI 660
             FA GAG+V+P+RA DPGLVYD    DY+ YLC L Y   ++        TC S    +
Sbjct: 619 TAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVL 678

Query: 661 REGDLNYPSFAVTL--GASQTS---KRTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNF 720
             GDLNYPSFAV L  GA+  +   KRTVTNVG     Y + V+ P GV V V P+ L F
Sbjct: 679 SPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKF 738

Query: 721 SRVNEKVTYSVSFSRDILVITPSEFSEGYLVWVSDKHFVRSPISVKFE 743
            +  E+++Y+V++  +      S  S G LVW+ DK+ VRSPI+V +E
Sbjct: 739 QKARERLSYTVTYDAE-ASRNSSSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Lcy02g014550 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 580.1 bits (1494), Expect = 2.5e-165
Identity = 346/757 (45.71%), Postives = 458/757 (60.50%), Query Frame = 0

Query: 23  LQTYIVHVE---QPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFS 82
           L++YIVHV+   +P L  S +     +W+ S L       SS + + LLYSY   + GFS
Sbjct: 30  LESYIVHVQRSHKPSLFSSHN-----NWHVSLLRS---LPSSPQPATLLYSYSRAVHGFS 89

Query: 83  ARLTEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVL 142
           ARL+  Q  A+      IS  P+    +HTT+TP +LG ++  GLW +SN+G+ VI+GVL
Sbjct: 90  ARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVL 149

Query: 143 DTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNR---------- 202
           DTGI P HPSF D G+   P+ WKG CE   +F AS CN+KLIGAR F R          
Sbjct: 150 DTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTK 209

Query: 203 --ASSAIQSPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPK 262
             A+   +SP D +GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C   
Sbjct: 210 KHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTG 269

Query: 263 GCSSSDILAALDAAIDDGVDVLSLSLG--GRSIPFFLDNIAIGSFAAIQKGIFVSCSAGN 322
           GC  SDILAA+D A+ DGV V+SLS+G  G +  +  D+IAIG+F A + GI VSCSAGN
Sbjct: 270 GCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGN 329

Query: 323 SGPSESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYA 382
           SGP+  T +N APWILTVGAS  DR   A A  G+G+V  G SLY     P + L LVY 
Sbjct: 330 SGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVY- 389

Query: 383 GSSGTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDG 442
             SG  GS  C  G L++  V GK+V+C+RGG   R+ KG  VK AGGA MI+ N    G
Sbjct: 390 --SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESG 449

Query: 443 FSTAAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRG 502
               A++H++PAT V   AG +I+ YI +S +P A ISF GT+IG +  SP +A+FSSRG
Sbjct: 450 EELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRG 509

Query: 503 PSRISPGILKPDITGPGVNILAAWP---FPLDNNTDTKST-FNVISGTSMSCPHLSGIAA 562
           P+ ++P ILKPD+  PGVNILA W     P D + D +   FN+ISGTSMSCPH+SG+AA
Sbjct: 510 PNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAA 569

Query: 563 LVKSTHPNWSPAAIKSAIMTSSDITNPRGKSIVDQDL-KPANFFAMGAGHVNPSRAADPG 622
           L++  HP+WSPAAIKSA++T++      G+ I D    K +N F  GAGHV+P++A +PG
Sbjct: 570 LLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPG 629

Query: 623 LVYDIQPDDYIPYLC-------GLYADAQVATIVHKLVTCSTVTRIREGDLNYPSFAV-- 682
           LVYDI+  +Y+ +LC       G+    Q  T+      C T      GDLNYPSF+V  
Sbjct: 630 LVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD---ACETSKLRTAGDLNYPSFSVVF 689

Query: 683 -TLGASQTSKRTVTNVGDAM-SNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSFSR 742
            + G     KR V NVG  + + Y + VK+P  V + V+P  L FS+    + Y V+F  
Sbjct: 690 ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKS 749

BLAST of Lcy02g014550 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 565.8 bits (1457), Expect = 5.0e-161
Identity = 334/744 (44.89%), Postives = 448/744 (60.22%), Query Frame = 0

Query: 24  QTYIV---HVEQPELGISGDKIDLESWYTSFLSETTITTSSNEKSRLLYSYRNVMSGFSA 83
           +TYI+   H ++PE  ++        WYTS L         N +S LLY+Y     GFSA
Sbjct: 28  KTYIIRVNHSDKPESFLTH-----HDWYTSQL---------NSESSLLYTYTTSFHGFSA 87

Query: 84  RL-TEEQVKAMEEKDGFISARPESILNLHTTYTPKYLGLNEQFGLWKDSNFGKGVIIGVL 143
            L + E    +   +  +    + +  LHTT TP++LGLN +FG+    +   GVIIGVL
Sbjct: 88  YLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVL 147

Query: 144 DTGITPAHPSFHDDGMPLPPAKWKGRCEEEEEFGASICNKKLIGARTFNRASSAIQ---- 203
           DTG+ P   SF D  MP  P+KWKG CE   +F + +CNKKLIGAR+F++          
Sbjct: 148 DTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGF 207

Query: 204 -------SPIDDDGHGTHTASTAAGAFVEGAQALGNARGKAAGMAPLAHIAMYKVCSPKG 263
                  SP D DGHGTHT++TAAG+ V  A  LG A G A GMA  A +A YKVC   G
Sbjct: 208 SSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 267

Query: 264 CSSSDILAALDAAIDDGVDVLSLSLGGRSIPFFLDNIAIGSFAAIQKGIFVSCSAGNSGP 323
           C  SDILAA+D AI DGVDVLSLSLGG S P++ D IAIG+F+A+++G+FVSCSAGNSGP
Sbjct: 268 CFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGP 327

Query: 324 SESTLSNDAPWILTVGASDTDRTIVALAKLGNGEVHFGESLYQPRDFPSTYLPLVYAGSS 383
           + ++++N APW++TVGA   DR   A A LGNG+   G SLY      +  L LVY    
Sbjct: 328 TRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-NKG 387

Query: 384 GTKGSEFCVQGSLSNMNVRGKVVVCERGGGIGRIAKGMVVKNAGGAAMIVINQQPDGFST 443
            +  S  C+ GSL +  VRGK+VVC+RG    R+ KG VV++AGG  MI+ N    G   
Sbjct: 388 NSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEEL 447

Query: 444 AAEAHVLPATHVSYTAGLRIKSYISSSQNPIASISFEGTVIGDNNFSPAMASFSSRGPSR 503
            A++H+LPA  V    G  ++ Y+ S   P A + F+GTV+ D   SP +A+FSSRGP+ 
Sbjct: 448 VADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNT 507

Query: 504 ISPGILKPDITGPGVNILAAW-----PFPLDNNTDTKSTFNVISGTSMSCPHLSGIAALV 563
           ++P ILKPD+ GPGVNILA W     P  LD ++  ++ FN++SGTSMSCPH+SG+A L+
Sbjct: 508 VTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDS-RRTQFNIMSGTSMSCPHISGLAGLL 567

Query: 564 KSTHPNWSPAAIKSAIMTSS---DITNPRGKSIVDQDLKPANFFAMGAGHVNPSRAADPG 623
           K+ HP WSP+AIKSA+MT++   D TN       D  L  +N +A G+GHV+P +A  PG
Sbjct: 568 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL--SNPYAHGSGHVDPQKALSPG 627

Query: 624 LVYDIQPDDYIPYLCGL--YADAQVATIVHKLVTCSTVTRIREGDLNYPSFAVTLGASQT 683
           LVYDI  ++YI +LC L    D  VA +    V CS       G LNYPSF+V  G  + 
Sbjct: 628 LVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS-DPGQLNYPSFSVLFGGKRV 687

Query: 684 SK--RTVTNVGDAMSNYSLVVKAPLGVSVTVTPRTLNFSRVNEKVTYSVSF--SRDILVI 739
            +  R VTNVG A S Y + V     V ++V P  L+F  V EK  Y+V+F   + + + 
Sbjct: 688 VRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMT 747