Lcy02g013270 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g013270
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	Chr02: 38902708 .. 38904641 (+)
RNA-Seq Expression	Lcy02g013270
Synteny	Lcy02g013270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGTACTTGTCATTTCTTTCTGAAACAACGACAATATAATGGCGGCGAAAATTAGATCATTATTGTGTGTAATTGGTATAATGGTGTTGTGTTTGTTGGGCATTTCTGAATCGTTACCTCTGTCCACAAGAGGAAGATGGATCGTAGACGCTCTGACTGGCCAACGTGTGAAGCTGAGCTGCGTCAATCTGGTGTCCCATGCACAGTCCATGGTGGCACAAGGGCTGGACAAGCGTCCACTCAAGGAGTTGGCCGCTGAGATTAGCAGCCGCCAGTTCAATTGTGTGCGTCTCACGTGGTCCATCCACATGTTCACTCGTCACCCCTTGGAGACCATTGGTGAAGTCTTTGATCTCCTTGATATCTCCGATGTCAAAAGTGGAGTTCAGAAGCACAACCCTGAGATATTAAACATGACGGTTGTTCAGGCTTTTGAAACTGTCATCAATGGCTTGGGCTCGGAGGGTATTATGGTCATTTTGGACAACCACATCAGCCAACCCAGATGGTGTTGCTCTCTCAACGATGGAAATGGCTTCTTTGGTGACCGCAATTTCAACCCCTATGAATGGCTTAGAGGCTTGGCCTTTGTGGCTCGCCATTTCACTTATTGCAATCCCCATGTGAGTTATATTATATTATATTTGCTATATATATATGCTAACATGAATTAATTTAATTAATTATTTATAAATTAAATAGGTGATAGGAATGAGCCTAAGAAACGAGCTCCGAGGACCATTTACAAACACGGAGGTGTGGTACAAATACATATGGGAAGGAAGCCATCTGGTCCACAGAATAAACCCTAATCTCCTTGTAATCATCTCGGGTCTAAACTACGACAACGATCTAAGCCACCTGAAGAAAAGGCCACTAGACTACACCTTAAACAACAAAGTGGTCTTGGAGGCACATCTCTACTCCTTCAGTGGCGACCCAGAAAGCAAGTTCGTCAAGAAGCCATTGAACATAATATGCGACCAAATCATGGACAAGTTCGAAAGGGAAGCAGGCTTCGTAGTGGACATGGACAATAATCCATATCCATTGTTCCTCAGTGAATTTGGGTACGACCAATCCCGCGCCAACGAGGCCGAGAATCGATTCATGAGCTGCTTCTTGGCTCGCATTGCTGCCAAAGATATCGACTGGGCTCTCTGGGCTTTCCAAGGAAGCTACATGTACAGACAAGGCCGTGAAGATCCTGATGAAAGCTTTGGCGTTATGGATCATTCTTGGACTAAAGATCGAAACCCTAAACTCAGGCAAATGCTTCAGCTTGCCCAGAGAATTAACCAAGGTTACGTTACAAAGATTACATTTTTAATTTACCAACTGGCCTTTTTCTATTTTGTCTTTTTCGTTAACGAAAGCTCTTTGATTTTATTTTGCAGATCCAAATTCGAAAGCGCCGATGTCGTACATAATGCTCCATCCGGTGTCGGGCCAATGCGTCAAATCCGACGGCAAGGGAGGAATTCAACTCGGTGACTGCACGGCGCCGACCACCCAGTGGAGCCACAGCGGAGATGCAAGTCCGATGAAGTTGGTTTCCAATGGGCAGTGTTTGAAGTCGGCCGGAGATGGGAAACCACCGCTGGTGTCGGCCAACTGCTCCGCCGATGGGAGCACATGGACGGTGGCTTCGAGAGCCAAGCTTCAATTGGCCACCAAAATTGGTGGTGATAATTTTTGCTTGGAGAAGGATTCTGATACTTCCATTGTTGTGAAGAAATGCATTTGCCTTAAAGATGAGTGGAATTGCATGGATGATCCTCAGAGCCAATGGTTCAAGCTTGTTCCCACAAACGTTGCTTAGTTATTTGTTTAGGAATTTAGAATTTATTTTTGGAGGAGTTAAGATTATTTAGGATTTTCGAATTTAGTTTTAGTCTGACGGTATTTAATAATTATTGTTACTCGGCATGAA

mRNA sequence

CTCGTACTTGTCATTTCTTTCTGAAACAACGACAATATAATGGCGGCGAAAATTAGATCATTATTGTGTGTAATTGGTATAATGGTGTTGTGTTTGTTGGGCATTTCTGAATCGTTACCTCTGTCCACAAGAGGAAGATGGATCGTAGACGCTCTGACTGGCCAACGTGTGAAGCTGAGCTGCGTCAATCTGGTGTCCCATGCACAGTCCATGGTGGCACAAGGGCTGGACAAGCGTCCACTCAAGGAGTTGGCCGCTGAGATTAGCAGCCGCCAGTTCAATTGTGTGCGTCTCACGTGGTCCATCCACATGTTCACTCGTCACCCCTTGGAGACCATTGGTGAAGTCTTTGATCTCCTTGATATCTCCGATGTCAAAAGTGGAGTTCAGAAGCACAACCCTGAGATATTAAACATGACGGTTGTTCAGGCTTTTGAAACTGTCATCAATGGCTTGGGCTCGGAGGGTATTATGGTCATTTTGGACAACCACATCAGCCAACCCAGATGGTGTTGCTCTCTCAACGATGGAAATGGCTTCTTTGGTGACCGCAATTTCAACCCCTATGAATGGCTTAGAGGCTTGGCCTTTGTGGCTCGCCATTTCACTTATTGCAATCCCCATGTGATAGGAATGAGCCTAAGAAACGAGCTCCGAGGACCATTTACAAACACGGAGGTGTGGTACAAATACATATGGGAAGGAAGCCATCTGGTCCACAGAATAAACCCTAATCTCCTTGTAATCATCTCGGGTCTAAACTACGACAACGATCTAAGCCACCTGAAGAAAAGGCCACTAGACTACACCTTAAACAACAAAGTGGTCTTGGAGGCACATCTCTACTCCTTCAGTGGCGACCCAGAAAGCAAGTTCGTCAAGAAGCCATTGAACATAATATGCGACCAAATCATGGACAAGTTCGAAAGGGAAGCAGGCTTCGTAGTGGACATGGACAATAATCCATATCCATTGTTCCTCAGTGAATTTGGGTACGACCAATCCCGCGCCAACGAGGCCGAGAATCGATTCATGAGCTGCTTCTTGGCTCGCATTGCTGCCAAAGATATCGACTGGGCTCTCTGGGCTTTCCAAGGAAGCTACATGTACAGACAAGGCCGTGAAGATCCTGATGAAAGCTTTGGCGTTATGGATCATTCTTGGACTAAAGATCGAAACCCTAAACTCAGGCAAATGCTTCAGCTTGCCCAGAGAATTAACCAAGATCCAAATTCGAAAGCGCCGATGTCGTACATAATGCTCCATCCGGTGTCGGGCCAATGCGTCAAATCCGACGGCAAGGGAGGAATTCAACTCGGTGACTGCACGGCGCCGACCACCCAGTGGAGCCACAGCGGAGATGCAAGTCCGATGAAGTTGGTTTCCAATGGGCAGTGTTTGAAGTCGGCCGGAGATGGGAAACCACCGCTGGTGTCGGCCAACTGCTCCGCCGATGGGAGCACATGGACGGTGGCTTCGAGAGCCAAGCTTCAATTGGCCACCAAAATTGGTGGTGATAATTTTTGCTTGGAGAAGGATTCTGATACTTCCATTGTTGTGAAGAAATGCATTTGCCTTAAAGATGAGTGGAATTGCATGGATGATCCTCAGAGCCAATGGTTCAAGCTTGTTCCCACAAACGTTGCTTAGTTATTTGTTTAGGAATTTAGAATTTATTTTTGGAGGAGTTAAGATTATTTAGGATTTTCGAATTTAGTTTTAGTCTGACGGTATTTAATAATTATTGTTACTCGGCATGAA

Coding sequence (CDS)

ATGGCGGCGAAAATTAGATCATTATTGTGTGTAATTGGTATAATGGTGTTGTGTTTGTTGGGCATTTCTGAATCGTTACCTCTGTCCACAAGAGGAAGATGGATCGTAGACGCTCTGACTGGCCAACGTGTGAAGCTGAGCTGCGTCAATCTGGTGTCCCATGCACAGTCCATGGTGGCACAAGGGCTGGACAAGCGTCCACTCAAGGAGTTGGCCGCTGAGATTAGCAGCCGCCAGTTCAATTGTGTGCGTCTCACGTGGTCCATCCACATGTTCACTCGTCACCCCTTGGAGACCATTGGTGAAGTCTTTGATCTCCTTGATATCTCCGATGTCAAAAGTGGAGTTCAGAAGCACAACCCTGAGATATTAAACATGACGGTTGTTCAGGCTTTTGAAACTGTCATCAATGGCTTGGGCTCGGAGGGTATTATGGTCATTTTGGACAACCACATCAGCCAACCCAGATGGTGTTGCTCTCTCAACGATGGAAATGGCTTCTTTGGTGACCGCAATTTCAACCCCTATGAATGGCTTAGAGGCTTGGCCTTTGTGGCTCGCCATTTCACTTATTGCAATCCCCATGTGATAGGAATGAGCCTAAGAAACGAGCTCCGAGGACCATTTACAAACACGGAGGTGTGGTACAAATACATATGGGAAGGAAGCCATCTGGTCCACAGAATAAACCCTAATCTCCTTGTAATCATCTCGGGTCTAAACTACGACAACGATCTAAGCCACCTGAAGAAAAGGCCACTAGACTACACCTTAAACAACAAAGTGGTCTTGGAGGCACATCTCTACTCCTTCAGTGGCGACCCAGAAAGCAAGTTCGTCAAGAAGCCATTGAACATAATATGCGACCAAATCATGGACAAGTTCGAAAGGGAAGCAGGCTTCGTAGTGGACATGGACAATAATCCATATCCATTGTTCCTCAGTGAATTTGGGTACGACCAATCCCGCGCCAACGAGGCCGAGAATCGATTCATGAGCTGCTTCTTGGCTCGCATTGCTGCCAAAGATATCGACTGGGCTCTCTGGGCTTTCCAAGGAAGCTACATGTACAGACAAGGCCGTGAAGATCCTGATGAAAGCTTTGGCGTTATGGATCATTCTTGGACTAAAGATCGAAACCCTAAACTCAGGCAAATGCTTCAGCTTGCCCAGAGAATTAACCAAGATCCAAATTCGAAAGCGCCGATGTCGTACATAATGCTCCATCCGGTGTCGGGCCAATGCGTCAAATCCGACGGCAAGGGAGGAATTCAACTCGGTGACTGCACGGCGCCGACCACCCAGTGGAGCCACAGCGGAGATGCAAGTCCGATGAAGTTGGTTTCCAATGGGCAGTGTTTGAAGTCGGCCGGAGATGGGAAACCACCGCTGGTGTCGGCCAACTGCTCCGCCGATGGGAGCACATGGACGGTGGCTTCGAGAGCCAAGCTTCAATTGGCCACCAAAATTGGTGGTGATAATTTTTGCTTGGAGAAGGATTCTGATACTTCCATTGTTGTGAAGAAATGCATTTGCCTTAAAGATGAGTGGAATTGCATGGATGATCCTCAGAGCCAATGGTTCAAGCTTGTTCCCACAAACGTTGCTTAG

Protein sequence

MAAKIRSLLCVIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKIGGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA

Homology

[Close]

BLAST of Lcy02g013270 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.8e-118
Identity = 221/544 (40.62%), Postives = 329/544 (60.48%), Query Frame = 0


Query: 7   SLLCVIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKR 66
           +L  +  +++L +   S SLPL TRGRWIVD  TG RVKL+CVN V H +  + +GL++ 
Sbjct: 10  ALRLLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRL 69

Query: 67  PLKELAAEISSRQFNCVRLTWSIHMFTR--HPLETIGEVFDLLDISDVKSGVQKHNPEIL 126
           P+  +A  ISS  FNCVRLT+SIHM TR  +   T+ + F  L++++  SG++ +NPE+L
Sbjct: 70  PVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELL 129

Query: 127 NMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAF 186
           ++  V A+  V+  L   G+MVILDNH+S+P+WCC+++DGNGFFGDR FNP  W+ GL  
Sbjct: 130 DLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGL 189

Query: 187 VARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDN 246
           +A +F    P+V+ MSLRNELRG  +    W +++  G+  VH+ NP +LVI+SGL +D 
Sbjct: 190 MATYFNN-TPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDT 249

Query: 247 DLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVD 306
           DLS L   P+      K+V E H YSF G P    +    N +C     +F+   GFV  
Sbjct: 250 DLSFLPVLPVTLPFKEKIVYEGHWYSF-GVPWRTGLP---NDVCKNETGRFKSNVGFVTS 309

Query: 307 MDN-NPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGRE- 366
             N    PLF+SEFG DQ   N+ +NR+++C LA +A +D+DWALW   GSY YR  ++ 
Sbjct: 310 SANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQP 369

Query: 367 --DPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPN-SKAPMSYIMLHPVSGQCVKSD 426
             D +E++G  +H W++ RNP     L+  Q+  QDP  +  P   I+ HP SG CV+S 
Sbjct: 370 VKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESG 429

Query: 427 GKGGIQLGDCTAPTTQWSHSGDA-SPMKLVSNGQCLKSAGDGKPPLVSANCSADGST-WT 486
               + LG C +  ++W++      P+ L+ +  C+ + G+G P +++ NCSA  +T W+
Sbjct: 430 IGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWS 489

Query: 487 VASRAKLQLATKIGGDN-----FCLEKDSDTSIVVKKCICLKDEWNCMD--DPQSQWFKL 535
             S A+LQL T++ G +      CL+      I   +CIC+ D  +C    +P+ QWFK+
Sbjct: 490 TVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDS-HCYPKLNPEKQWFKV 547

[Close]

BLAST of Lcy02g013270 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 6.6e-11
Identity = 76/379 (20.05%), Postives = 143/379 (37.73%), Query Frame = 0


Query: 30  TRGRWIVDALTGQRVKLSCVNLVS-HAQSMVAQGLDKRPLKELAAEISSRQFNCVRLTWS 89
           T+G  IVD  +G+    + +N       +    GL  R + ++  ++    +N +RL +S
Sbjct: 41  TQGNKIVDE-SGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 90  IHMFTRHPLETIGEVFDLLDISDVKSGVQKH-NPEILNMTVVQAFETVINGLGSEGIMVI 149
             +F               D S     +  H NP+++ +  +Q  + +I   G  GI +I
Sbjct: 101 NQLF---------------DSSSRPDSIDYHKNPDLVGLNPIQIMDKLIEKAGQRGIQII 160

Query: 150 LDNHISQPRWCCSLNDGNG----FFGDRNFNPYEWLRGLAFVARHFTYCNPHVIGMSLRN 209
           LD H            G+G     +    +    W+     +A  +   NP VIG  L N
Sbjct: 161 LDRH----------RPGSGGQSELWYTSQYPESRWISDWKMLADRYKN-NPTVIGADLHN 220

Query: 210 ELRGPFT-----NTEVWYKYIWEGSHLVHRINPNLLVIISGLNYD-----------NDLS 269
           E  G  +      +  W        + +  +NPN L+++ G++++            +L+
Sbjct: 221 EPHGQASWGTGNASTDWRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLT 280

Query: 270 HLKKRPLDYTLNNKVVLEAHLY--SFSGDPESKFVKKPLNI--ICDQIMDKFEREAGFVV 329
            +   P+   + N+VV   H Y    S  P       P N+  I DQ      ++     
Sbjct: 281 GVANYPVVLDVPNRVVYSPHDYGPGVSSQPWFNDPAFPSNLPAIWDQTWGYISKQ----- 340

Query: 330 DMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGRED 383
               N  P+ + EFG      +  E ++ +  +  I A ++ +  W+            +
Sbjct: 341 ----NIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSL---------NPN 374

[Close]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL
Match: A0A0A0KNT6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.2e-250
Identity = 416/526 (79.09%), Postives = 468/526 (88.97%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I I+ L LL +S+SLPLSTRGRWIVD+ TGQRVKLSCVNLVSHAQSMVAQGLDKRPLK+
Sbjct: 7   LISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LA EI SR +NCVRLTWS++MFTR+P ETIG+V D LDI+ VK+GV+KHNP+ LNMTV  
Sbjct: 67  LANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTVTS 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TV++GLG+ G+MVILDNHISQPRWCCSL+DGNGFFGDRNF P EWLRGLA+VARHF+
Sbjct: 127 AFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARHFS 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
           + +P V+GMSLRNELRG   N  VW KY+  GSHL+HRINP LLV+ISGLNYDNDLS+LK
Sbjct: 187 W-HPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           K+PL Y LNNKVVLEAHLYSFSG+PESKFVKKPLNI C+Q+MDKFEREAGFVVDM  +PY
Sbjct: 247 KKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM-KDPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYD    N+A+NRFMSCFLARI  KDIDWA WAFQGSYMYRQG++D DESFG+
Sbjct: 307 PLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDESFGI 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MD SWTKDR+P+L+QMLQLA+RINQDPNSK PMSYIMLHPVSGQCVK DGKGGI+LGDC 
Sbjct: 367 MDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELGDCE 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
            PT  W H+GD SPMKL  NGQCLKSAGDGKPP+VSA CS DGS+WTVAS+AKLQL+TK 
Sbjct: 427 TPTL-WDHTGDGSPMKL-WNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTKS 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           GG+N CLEK+SDTSIVVKKCICLKDEWNC DDPQSQWFKLVPTNVA
Sbjct: 487 GGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

[Close]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL
Match: A0A5A7TIV3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002130 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.9e-245
Identity = 407/526 (77.38%), Postives = 462/526 (87.83%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I ++ L LL +S+SLPLSTRGRWIVD+ TGQRVKLSCVNLVSHAQSMVAQGLDKRPLK+
Sbjct: 7   LISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LA EI SR +NCVRLTWS++MFTRHP ETIG+V D LDI+ VK+GV+KHNP+ILNMTV  
Sbjct: 67  LANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTVTS 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TV++GLG+ G+MVI+DNHISQPRWCCSL+DGNGFFGDRNF P EWLRGLAF      
Sbjct: 127 AFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF------ 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
                V+GMSLRNELRG   N +VW KY+ +GSHL+HRINP LLV+ISGLNYDNDLS+LK
Sbjct: 187 -----VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           K+PL Y L+NKVVLEAHLYSFSG+PESKFVKKPLNI C+Q+MDKFEREAGFVVDM  NPY
Sbjct: 247 KKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM-KNPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYD    N+A+NRFMSCFLARI  KDI+WA WAFQGSYMYRQG++D DESFG+
Sbjct: 307 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MD +WTKDR+P+L+QML+LA+RINQDPNSKAP+SYIMLHPVSGQCV+ DGKGGI+LGDC 
Sbjct: 367 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
            PT  W H+GD SPMKL SNGQCLKSAGDGKPP+VSA CS DGS+WTVAS+AKLQLATK 
Sbjct: 427 KPTL-WDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKS 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           GG+  CLEK+SDTSIVVKKCICLKDEWNC DDPQ QWFKLVPTNVA
Sbjct: 487 GGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL
Match: A0A1S3AUC3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.9e-245
Identity = 407/526 (77.38%), Postives = 462/526 (87.83%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I ++ L LL +S+SLPLSTRGRWIVD+ TGQRVKLSCVNLVSHAQSMVAQGLDKRPLK+
Sbjct: 7   LISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LA EI SR +NCVRLTWS++MFTRHP ETIG+V D LDI+ VK+GV+KHNP+ILNMTV  
Sbjct: 67  LANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTVTS 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TV++GLG+ G+MVI+DNHISQPRWCCSL+DGNGFFGDRNF P EWLRGLAF      
Sbjct: 127 AFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF------ 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
                V+GMSLRNELRG   N +VW KY+ +GSHL+HRINP LLV+ISGLNYDNDLS+LK
Sbjct: 187 -----VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           K+PL Y L+NKVVLEAHLYSFSG+PESKFVKKPLNI C+Q+MDKFEREAGFVVDM  NPY
Sbjct: 247 KKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM-KNPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYD    N+A+NRFMSCFLARI  KDI+WA WAFQGSYMYRQG++D DESFG+
Sbjct: 307 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MD +WTKDR+P+L+QML+LA+RINQDPNSKAP+SYIMLHPVSGQCV+ DGKGGI+LGDC 
Sbjct: 367 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
            PT  W H+GD SPMKL SNGQCLKSAGDGKPP+VSA CS DGS+WTVAS+AKLQLATK 
Sbjct: 427 KPTL-WDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKS 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           GG+  CLEK+SDTSIVVKKCICLKDEWNC DDPQ QWFKLVPTNVA
Sbjct: 487 GGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL
Match: A0A6J1JQD4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.2e-242
Identity = 405/479 (84.55%), Postives = 441/479 (92.07%), Query Frame = 0


Query: 58  MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQ 117
           MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTR+  ETIG+V D LDI DVK+GV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRYAYETIGDVLDGLDIGDVKNGVE 60

Query: 118 KHNPEILNMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYE 177
           KHNPEIL M+VV+AF+TVIN LGSEGIMVILDNHISQPRWCCSL+DGNGFFGDRNFNP E
Sbjct: 61  KHNPEILKMSVVKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 178 WLRGLAFVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVII 237
           WL+GL+FVA  FT CNPHVIGMSLRNELRGPFTN + W  Y+ +GS L+HRINPN+LVII
Sbjct: 121 WLQGLSFVATQFT-CNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILVII 180

Query: 238 SGLNYDNDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFER 297
           SGLNYDNDLS+LKKRPL YTL+NKVVLEAHLYSFSGD ESKFVKKPLNIIC++IM+KFER
Sbjct: 181 SGLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFER 240

Query: 298 EAGFVVDMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMY 357
           EAGFVVDM+ NPYPLFLSEFGYDQS  NEAENRFMSCFLARIA KDIDWALWAFQGSYMY
Sbjct: 241 EAGFVVDME-NPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMY 300

Query: 358 RQGREDPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVK 417
           RQG  DPDESFGVMDHSWTKDRNPKL Q+LQL +RINQDPNSKAPMSYI+LH VSGQCVK
Sbjct: 301 RQGHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIVLHSVSGQCVK 360

Query: 418 SDGKGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWT 477
           SD KGGI+LGDCTAP TQW+H+GDASPMKL+SNGQCLKSAGDGK P+VSA CS DGS WT
Sbjct: 361 SDNKGGIELGDCTAP-TQWNHTGDASPMKLLSNGQCLKSAGDGKSPVVSAECSGDGSNWT 420

Query: 478 VASRAKLQLATKIGGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           V+S+AKLQLATKIG DNFCLEK+SD SIV+KKCICL DE +CMDDPQ+QWFKLVPTNV+
Sbjct: 421 VSSKAKLQLATKIGEDNFCLEKESDASIVLKKCICL-DEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL
Match: A0A6J1GQA8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456519 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.3e-238
Identity = 397/479 (82.88%), Postives = 437/479 (91.23%), Query Frame = 0


Query: 58  MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQ 117
           MVAQGLDKRPLKELAAEISSRQFNCVRLTWS+HMFTR+  ETIG+V D LDISDVK+GV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDISDVKNGVE 60

Query: 118 KHNPEILNMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYE 177
           KHN E+L M+V +AF+TVIN LGSEGIMVILDNHISQ RWCCSL+DGNGFFGDRNFNP E
Sbjct: 61  KHNSEVLKMSVAKAFQTVINCLGSEGIMVILDNHISQSRWCCSLDDGNGFFGDRNFNPNE 120

Query: 178 WLRGLAFVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVII 237
           W +GL+FVA  FT CNPHVIGMSLRNELRGPFTN + W  Y+ +GS L+HRINPN+L+II
Sbjct: 121 WFQGLSFVATQFT-CNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIII 180

Query: 238 SGLNYDNDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFER 297
           SGLNYDNDLS+LKKRPL YTL+NKVVLEAHLYSFSGD ESKFVKKPLNIIC++IM+KFER
Sbjct: 181 SGLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFER 240

Query: 298 EAGFVVDMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMY 357
           EAGFVVDM+ NPYPLFLSEFGYDQS  NEAENRFMSCFLARIA KDIDWALWAFQGSYMY
Sbjct: 241 EAGFVVDME-NPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMY 300

Query: 358 RQGREDPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVK 417
           RQG  DPDESFGVMDHSWTKDRNPKL Q+LQL +RINQDPNSKAPMSYIMLH VSGQCVK
Sbjct: 301 RQGHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVK 360

Query: 418 SDGKGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWT 477
           SD KGGI+LGDCT+P TQW+H+GDASPMKL+SNGQCLKSAGDG+ P+VSA CS DGS WT
Sbjct: 361 SDNKGGIELGDCTSP-TQWNHTGDASPMKLLSNGQCLKSAGDGQSPVVSAECSGDGSNWT 420

Query: 478 VASRAKLQLATKIGGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           V+S+AKLQLATK+G DNFCLEK SD SIV+KKCICL DE +CMDDPQ+QWFKLVPTNV+
Sbjct: 421 VSSKAKLQLATKVGEDNFCLEKKSDASIVLKKCICL-DEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of Lcy02g013270 vs. NCBI nr
Match: KAG6601845.1 (hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sororia] >KAG6601853.1 hypothetical protein SDJN03_07086, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-257
Identity = 428/526 (81.37%), Postives = 477/526 (90.68%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I +++  L   S+SLPL TRGRWI+DA TG+RVKL+CVNLVSHAQ+MVAQGLDKRPLKE
Sbjct: 7   LIALILFSLFSFSQSLPLCTRGRWIIDAQTGKRVKLNCVNLVSHAQTMVAQGLDKRPLKE 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LAAEISSRQFNCVRLTWS+HMFTR+  ETIG+V D LDI DVK+GV+KHNPEIL M+V +
Sbjct: 67  LAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDIGDVKNGVEKHNPEILKMSVAK 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TVIN LGSEGIMVILDNHISQPRWCCSL+DGNGFFGDRNFNP EW +GL+FVA  FT
Sbjct: 127 AFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWFQGLSFVATQFT 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
            CNPHVIGMSLRNELRGPFTN + W  Y+ +GS L+HRINPN+L+IISGLNYDNDLS+LK
Sbjct: 187 -CNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIIISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           KRPL YTL+NKVVLEAHLYSFSGD ESKFVKKPLNIIC++IM+KFEREAGFVVDM+ NPY
Sbjct: 247 KRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDME-NPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYDQS  NEAENRFMSCFLARIA KDIDWALWAFQGSYMYRQG  DPDESFGV
Sbjct: 307 PLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHTDPDESFGV 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MDHSWTKDRNPKL Q+LQL +RINQDPNSKAPMSYIMLH VSGQCV+SD KGGI+LGDCT
Sbjct: 367 MDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVRSDNKGGIELGDCT 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
           +P TQW+H+GDASPM L+SNGQCLKSAGDGK P+VSA CS DGS WTV+S+AKLQLATKI
Sbjct: 427 SP-TQWNHTGDASPMMLLSNGQCLKSAGDGKSPMVSAECSGDGSNWTVSSKAKLQLATKI 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           G DNFCLEK+S+ SIV+KKCICL DE +CMDDPQ+QWFKLVPTNV+
Sbjct: 487 GEDNFCLEKESNASIVLKKCICL-DEESCMDDPQTQWFKLVPTNVS 528

[Close]

BLAST of Lcy02g013270 vs. NCBI nr
Match: XP_038907081.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 897.9 bits (2319), Expect = 4.3e-257
Identity = 423/528 (80.11%), Postives = 476/528 (90.15%), Query Frame = 0


Query: 9   LCVIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 68
           L +I +++  LL +S+SLPLST GRWIVD+ TGQRVKLSCVNLVSHAQSM+AQGLDKR L
Sbjct: 5   LSLISLVLFSLLVLSQSLPLSTSGRWIVDSQTGQRVKLSCVNLVSHAQSMLAQGLDKRSL 64

Query: 69  KELAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTV 128
           K+LA +ISSR FNCVRLTWSIHMFTRH  ETIG+V D LDI  VKSGVQKHNPEILNMTV
Sbjct: 65  KDLANDISSRDFNCVRLTWSIHMFTRHAYETIGDVLDGLDIGKVKSGVQKHNPEILNMTV 124

Query: 129 VQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARH 188
             AF TVINGLG+ G+M++LDNHISQPRWCCSLNDGNGFFGDRNFNP EWL+GL +VARH
Sbjct: 125 SHAFRTVINGLGNAGLMIVLDNHISQPRWCCSLNDGNGFFGDRNFNPNEWLQGLTYVARH 184

Query: 189 FTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSH 248
           F+ C PHVIGMSLRNELRG   N + W+KY+  GSHL+H INPNLLV+ISGLNYDNDLSH
Sbjct: 185 FS-CQPHVIGMSLRNELRGS-NNVDEWHKYVRRGSHLIHNINPNLLVVISGLNYDNDLSH 244

Query: 249 LKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNN 308
           LKK+PL Y L+NKVVLEAHLYSFSGDPESKFVKKPLNI C+Q+M+KFEREAGFVVDM+ +
Sbjct: 245 LKKKPLGYKLHNKVVLEAHLYSFSGDPESKFVKKPLNIACNQVMEKFEREAGFVVDME-D 304

Query: 309 PYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESF 368
           PYPL++SEFGYD    N+A+NRFM+CFLAR+  KDIDWALWAFQGSYMYRQG+ED DESF
Sbjct: 305 PYPLWISEFGYDVRGGNKAQNRFMNCFLARLIGKDIDWALWAFQGSYMYRQGQEDRDESF 364

Query: 369 GVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGD 428
           GVMD++WTKDR+P+L+QMLQLA+RINQDPNSKA MSYIMLHPVSGQCVKSDGKGGI+LGD
Sbjct: 365 GVMDNTWTKDRSPQLKQMLQLAKRINQDPNSKASMSYIMLHPVSGQCVKSDGKGGIELGD 424

Query: 429 CTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLAT 488
           CT PT  W+H+GD +PMKL+SNGQCLKSAGDGKPP+VSA+CS DGS WTVAS+AKLQLAT
Sbjct: 425 CTTPTL-WNHTGDGTPMKLLSNGQCLKSAGDGKPPMVSADCSGDGSNWTVASKAKLQLAT 484

Query: 489 KIGGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           K GG+NFCLEK+SDTSIVVKKCICLKDEWNC DDPQSQWFKLVPTNVA
Sbjct: 485 KSGGENFCLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 528

[Close]

BLAST of Lcy02g013270 vs. NCBI nr
Match: XP_004143963.2 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 874.0 bits (2257), Expect = 6.6e-250
Identity = 416/526 (79.09%), Postives = 468/526 (88.97%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I I+ L LL +S+SLPLSTRGRWIVD+ TGQRVKLSCVNLVSHAQSMVAQGLDKRPLK+
Sbjct: 7   LISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LA EI SR +NCVRLTWS++MFTR+P ETIG+V D LDI+ VK+GV+KHNP+ LNMTV  
Sbjct: 67  LANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTVTS 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TV++GLG+ G+MVILDNHISQPRWCCSL+DGNGFFGDRNF P EWLRGLA+VARHF+
Sbjct: 127 AFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARHFS 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
           + +P V+GMSLRNELRG   N  VW KY+  GSHL+HRINP LLV+ISGLNYDNDLS+LK
Sbjct: 187 W-HPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           K+PL Y LNNKVVLEAHLYSFSG+PESKFVKKPLNI C+Q+MDKFEREAGFVVDM  +PY
Sbjct: 247 KKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM-KDPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYD    N+A+NRFMSCFLARI  KDIDWA WAFQGSYMYRQG++D DESFG+
Sbjct: 307 PLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDESFGI 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MD SWTKDR+P+L+QMLQLA+RINQDPNSK PMSYIMLHPVSGQCVK DGKGGI+LGDC 
Sbjct: 367 MDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELGDCE 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
            PT  W H+GD SPMKL  NGQCLKSAGDGKPP+VSA CS DGS+WTVAS+AKLQL+TK 
Sbjct: 427 TPTL-WDHTGDGSPMKL-WNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTKS 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           GG+N CLEK+SDTSIVVKKCICLKDEWNC DDPQSQWFKLVPTNVA
Sbjct: 487 GGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

[Close]

BLAST of Lcy02g013270 vs. NCBI nr
Match: XP_008437726.1 (PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK06112.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 856.3 bits (2211), Expect = 1.4e-244
Identity = 407/526 (77.38%), Postives = 462/526 (87.83%), Query Frame = 0


Query: 11  VIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 70
           +I ++ L LL +S+SLPLSTRGRWIVD+ TGQRVKLSCVNLVSHAQSMVAQGLDKRPLK+
Sbjct: 7   LISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 66

Query: 71  LAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQKHNPEILNMTVVQ 130
           LA EI SR +NCVRLTWS++MFTRHP ETIG+V D LDI+ VK+GV+KHNP+ILNMTV  
Sbjct: 67  LANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTVTS 126

Query: 131 AFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFT 190
           AF+TV++GLG+ G+MVI+DNHISQPRWCCSL+DGNGFFGDRNF P EWLRGLAF      
Sbjct: 127 AFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF------ 186

Query: 191 YCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLK 250
                V+GMSLRNELRG   N +VW KY+ +GSHL+HRINP LLV+ISGLNYDNDLS+LK
Sbjct: 187 -----VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYLK 246

Query: 251 KRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPY 310
           K+PL Y L+NKVVLEAHLYSFSG+PESKFVKKPLNI C+Q+MDKFEREAGFVVDM  NPY
Sbjct: 247 KKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM-KNPY 306

Query: 311 PLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGV 370
           PLFLSEFGYD    N+A+NRFMSCFLARI  KDI+WA WAFQGSYMYRQG++D DESFG+
Sbjct: 307 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 366

Query: 371 MDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLGDCT 430
           MD +WTKDR+P+L+QML+LA+RINQDPNSKAP+SYIMLHPVSGQCV+ DGKGGI+LGDC 
Sbjct: 367 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 426

Query: 431 APTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWTVASRAKLQLATKI 490
            PT  W H+GD SPMKL SNGQCLKSAGDGKPP+VSA CS DGS+WTVAS+AKLQLATK 
Sbjct: 427 KPTL-WDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKS 486

Query: 491 GGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           GG+  CLEK+SDTSIVVKKCICLKDEWNC DDPQ QWFKLVPTNVA
Sbjct: 487 GGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Lcy02g013270 vs. NCBI nr
Match: XP_022990540.1 (uncharacterized protein LOC111487386 [Cucurbita maxima])

HSP 1 Score: 847.0 bits (2187), Expect = 8.6e-242
Identity = 405/479 (84.55%), Postives = 441/479 (92.07%), Query Frame = 0


Query: 58  MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRHPLETIGEVFDLLDISDVKSGVQ 117
           MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTR+  ETIG+V D LDI DVK+GV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRYAYETIGDVLDGLDIGDVKNGVE 60

Query: 118 KHNPEILNMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYE 177
           KHNPEIL M+VV+AF+TVIN LGSEGIMVILDNHISQPRWCCSL+DGNGFFGDRNFNP E
Sbjct: 61  KHNPEILKMSVVKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 178 WLRGLAFVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVII 237
           WL+GL+FVA  FT CNPHVIGMSLRNELRGPFTN + W  Y+ +GS L+HRINPN+LVII
Sbjct: 121 WLQGLSFVATQFT-CNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILVII 180

Query: 238 SGLNYDNDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFER 297
           SGLNYDNDLS+LKKRPL YTL+NKVVLEAHLYSFSGD ESKFVKKPLNIIC++IM+KFER
Sbjct: 181 SGLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFER 240

Query: 298 EAGFVVDMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMY 357
           EAGFVVDM+ NPYPLFLSEFGYDQS  NEAENRFMSCFLARIA KDIDWALWAFQGSYMY
Sbjct: 241 EAGFVVDME-NPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMY 300

Query: 358 RQGREDPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVK 417
           RQG  DPDESFGVMDHSWTKDRNPKL Q+LQL +RINQDPNSKAPMSYI+LH VSGQCVK
Sbjct: 301 RQGHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIVLHSVSGQCVK 360

Query: 418 SDGKGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSADGSTWT 477
           SD KGGI+LGDCTAP TQW+H+GDASPMKL+SNGQCLKSAGDGK P+VSA CS DGS WT
Sbjct: 361 SDNKGGIELGDCTAP-TQWNHTGDASPMKLLSNGQCLKSAGDGKSPVVSAECSGDGSNWT 420

Query: 478 VASRAKLQLATKIGGDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLVPTNVA 537
           V+S+AKLQLATKIG DNFCLEK+SD SIV+KKCICL DE +CMDDPQ+QWFKLVPTNV+
Sbjct: 421 VSSKAKLQLATKIGEDNFCLEKESDASIVLKKCICL-DEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of Lcy02g013270 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.7e-108
Identity = 217/527 (41.18%), Postives = 305/527 (57.87%), Query Frame = 0


Query: 12  IGIMVLCLLGISESLPLSTRGRWIV-DALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKE 71
           I + +L  +  + + P ST  RWIV D   G+RVKL+CVN  SH ++ VA+GL K+PL  
Sbjct: 7   ISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDA 66

Query: 72  LAAEISSRQFNCVRLTWSIHMFTRHPLE---TIGEVFDLLDISDVKSGVQKHNPEILNMT 131
           +A +I S  FNCVRLTW +++ T        T+ +      + +  SG Q HNP IL++ 
Sbjct: 67  IAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLP 126

Query: 132 VVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVAR 191
           +++AF+ V+  L    +MVILDNHISQP WCCS NDGNGFFGD++ NP  W++GL  +A 
Sbjct: 127 LIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMAS 186

Query: 192 HFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLS 251
            F   + +V+GMSLRNELRGP  N + WYKY+ EG+  VH +NPN+LVI+SGLNY  DLS
Sbjct: 187 MFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLS 246

Query: 252 HLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDN 311
            L++RP + +   KVV E H Y F    E       LN IC +  +K  + +GF+++   
Sbjct: 247 FLRERPFEVSFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGFLLE--- 306

Query: 312 NPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDES 371
              PLF+SEFG DQ   N  +N+F+SCF+A  A +D+DW+LW   GSY  R+     DES
Sbjct: 307 KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDES 366

Query: 372 FGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSDGKGGIQLG 431
           +GV+D +W+  RN  + QM+   Q          P   IM HP +G C+       ++LG
Sbjct: 367 YGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCIVRKSLFQLKLG 426

Query: 432 DCTAPTTQWSHSGDASPMKLVSNGQCLKSAGDGKPPLVSANCSAD-GSTWTVASRAKLQL 491
            C   +  W  S             CLK+   GK   +    S    S W + S +K+QL
Sbjct: 427 SCNR-SESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQL 486

Query: 492 AT-KIGGDNFCLEKDSD-TSIVVKKCICLKDEWNCMDDPQSQWFKLV 532
           ++    G + CL+ D++  +IV   C CL+   +C  DP+SQWFKLV
Sbjct: 487 SSITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSC--DPRSQWFKLV 522

[Close]

BLAST of Lcy02g013270 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-107
Identity = 201/514 (39.11%), Postives = 299/514 (58.17%), Query Frame = 0


Query: 25  SLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKELAAEISSRQFNCVR 84
           S PLST  RWIVD   G RVKL C N  SH Q +VA+GL K+P+  +A +I    FNCVR
Sbjct: 32  SYPLSTSSRWIVDE-NGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 85  LTWSIHMFTRHPLE---TIGEVFDLLDISDVKSGVQKHNPEILNMTVVQAFETVINGLGS 144
           LTW + + T   L    T+ + F  L ++D   G Q +NP I+++ +++A++TV+  LG+
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 145 EGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFTYCNPHVIGMSL 204
             +MVILDNH+++P WCC+ +DGNGFFGD+ F+P  W+  L  +A  F   + +V+GMSL
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVS-NVVGMSL 211

Query: 205 RNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLKKRPLDYTLNNK 264
           RNELRGP  N   W+KY+ +G+  VH  N  +LVI+SGL++D DLS ++ RP+  +   K
Sbjct: 212 RNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLSFTGK 271

Query: 265 VVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPYPLFLSEFGYDQ 324
           +V E H YSFS D  S     P N IC +++++     G+++   N  +PLFLSEFG D+
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLL---NQGFPLFLSEFGIDE 331

Query: 325 SRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGVMDHSWTKDRNP 384
              N  +NR+  C     A  D+DW+LWA  GSY  RQG+   +E +GV+D  W   RN 
Sbjct: 332 RGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNS 391

Query: 385 KLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCV--KSDGKGGIQLGDCTAPTTQWSHS 444
              Q +   Q   Q P  +     ++ HP++G C+    D    + LG C + +  WS++
Sbjct: 392 SFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNS-SEPWSYT 451

Query: 445 GDASPMKLVSNGQCLKSAGDGKP-PLVSANCSADGSTWTVASRAKLQLA-TKIGGDNFCL 504
             A  +++     CL+S G   P  +   +CS  GS W   S +++ LA T     + CL
Sbjct: 452 KKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCL 511

Query: 505 EKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKLV 532
           + D+  ++V   C CL  + +C  +P SQWFK++
Sbjct: 512 DVDTANNVVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of Lcy02g013270 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.9e-106
Identity = 199/512 (38.87%), Postives = 296/512 (57.81%), Query Frame = 0


Query: 27  PLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKELAAEISSRQFNCVRLT 86
           PLST  RWI+D   GQRVKL+CVN  SH Q +VA+GL K+ + +LA +I +  FNCVR T
Sbjct: 4   PLSTNSRWIIDE-KGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 87  WSIHMFTRHPLE---TIGEVFDLLDISDVKSGVQKHNPEILNMTVVQAFETVINGLGSEG 146
           W + + T   L    T+ + F  L ++D  SG +  NP ++++ +++A++ V+  LG+  
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 147 IMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGLAFVARHFTYCNPHVIGMSLRN 206
           +MVILDNH+++P WCC  NDGNGFFGD  F+P  W+ GL  +A  F     +V+GMSLRN
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGAT-NVVGMSLRN 183

Query: 207 ELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYDNDLSHLKKRPLDYTLNNKVV 266
           ELRGP  N + W+KY+ +G+  VH  NPN+LVI+SGL+YD DLS ++ R ++ T   K+V
Sbjct: 184 ELRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLV 243

Query: 267 LEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVVDMDNNPYPLFLSEFGYDQSR 326
            E H YSF+    + +  K  N  C +I+   E   GF    +   +P+FLSEFG D   
Sbjct: 244 FELHRYSFTN--TNTWSSKNPNEACGEILKSIENGGGF----NLRDFPVFLSEFGIDLRG 303

Query: 327 ANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGREDPDESFGVMDHSWTKDRNPKL 386
            N  +NR++ C L   A  D+DW++W  QGSY  R+G     E +G++D  W + R+   
Sbjct: 304 KNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSF 363

Query: 387 RQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVKSD--GKGGIQLGDCTAPTTQWSHSGD 446
            Q L L     Q P S++ +  ++ HP++G C+         + LG C   +  WS++  
Sbjct: 364 LQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCN-ESQPWSYT-P 423

Query: 447 ASPMKLVSNGQCLKSAGDGKP-PLVSANCSADG-STWTVASRAKLQLATKIGGDNFCLEK 506
            + + L     CL+S G   P  L   +CS+   S W   S + + LA K   ++ CL+ 
Sbjct: 424 QNTLTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNSLCLDV 483

Query: 507 DSDTSIVVKKCICLKDEWNCMDDPQSQWFKLV 532
           D   +++   C C+K E +   DP SQWFK+V
Sbjct: 484 DETNNLMASNCKCVKGE-DSSCDPISQWFKIV 504

[Close]

BLAST of Lcy02g013270 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 348.6 bits (893), Expect = 9.0e-96
Identity = 200/538 (37.17%), Postives = 290/538 (53.90%), Query Frame = 0


Query: 1   MAAKIRSLLCVIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVA 60
           MA  I   + +   + L  L ++   PL T+ RWIV+   G RVKL+C N  SH + +VA
Sbjct: 1   MAKAIALTVFLFLFLSLISLTLATDYPLFTKSRWIVNN-KGHRVKLACANWPSHLKPVVA 60

Query: 61  QGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRHPLE---TIGEVFDLLDISDVKSGVQ 120
           +GL  +P+  ++ +I    FNCVRLTW + +     L    T+ + F+   +     G+ 
Sbjct: 61  EGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIY 120

Query: 121 KHNPEILNMTVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYE 180
            HNP I+N  ++  F+ V+  LG   +MVILDNH + P WCCS +D + FFGD  FNP  
Sbjct: 121 THNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDL 180

Query: 181 WLRGLAFVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVII 240
           W+ GL  +A  F     +V+GMSLRNELRG    ++ WYKY+ +G+  VH  NPN+LVI+
Sbjct: 181 WMLGLKKMATIFMNVK-NVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVIL 240

Query: 241 SGLNYDNDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFER 300
           SGLN+D DLS LK RP++ +   K+VLE H YSF+ D   ++    +N  C Q+  K  R
Sbjct: 241 SGLNFDADLSFLKDRPVNLSFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERR 300

Query: 301 EAGFVVDMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMY 360
             GFV+D     +PLFLSEFG DQ   +   NR+M+C LA  A KD+DWA+WA  G Y +
Sbjct: 301 TGGFVLDQG---FPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYF 360

Query: 361 RQGREDPDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCVK 420
           R+G+    E++G++D +W    N    + L + Q  +  P  K      + HP++G C+ 
Sbjct: 361 REGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLV 420

Query: 421 SDG---KGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLK-SAGDGKPPLVSANCSADG 480
                 +  + LG CT     WS+S          +  CL+     GK   +   C+   
Sbjct: 421 RKSHCHESELTLGPCT-KDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIE 480

Query: 481 STWTVASRAKLQLATKIG-GDNFCLEKDSDTSIVVKKCICLKDEWNCMDDPQSQWFKL 531
                 S  K+ L+     G   CL+ DSD ++V   C CL  +  C  +P SQWFK+
Sbjct: 481 Q----ISATKMHLSFNTSDGSLVCLDVDSDNNVVANSCNCLTGDTTC--EPASQWFKI 525

[Close]

BLAST of Lcy02g013270 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 4.5e-87
Identity = 196/536 (36.57%), Postives = 287/536 (53.54%), Query Frame = 0


Query: 10  CVIGIMVLCLLGISESLPLSTRGRWIVDALTGQRVKLSCVNLVSHAQSMVAQGLDKRPLK 69
           C+  + +     ++ S PLST+ RWIVD   GQRVKL+CVN  +H Q  VA+GL K+PL 
Sbjct: 8   CLFFLFISSTSKLTTSYPLSTKSRWIVDE-KGQRVKLACVNWPAHLQPTVAEGLSKQPLD 67

Query: 70  ELAAEISSRQFNCVRLTWSIHMFTRHPLE---TIGEVFDLLDISDVKSGVQKHNPEILNM 129
            ++ +I S  FNCVRLTW + + T   L    T+ + F+ L + +   G+Q HNP++L++
Sbjct: 68  SISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHL 127

Query: 130 TVVQAFETVINGLGSEGIMVILDNHISQPRWCCSLNDGNGFFGDRNFNPYEWLRGL---A 189
            +  AF+ V++ LG  G+MVILDNH++ P WCC  ND + FFG  +F+P  W +GL   A
Sbjct: 128 PLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMA 187

Query: 190 FVARHFTYCNPHVIGMSLRNELRGPFTNTEVWYKYIWEGSHLVHRINPNLLVIISGLNYD 249
            + R+FT    HVIGMSLRNE RG     ++W++++ +G+  VH  NP LLVI+SG+++D
Sbjct: 188 TLFRNFT----HVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFD 247

Query: 250 NDLSHLKKRPLDYTLNNKVVLEAHLYSFSGDPESKFVKKPLNIICDQIMDKFEREAGFVV 309
            +LS L+ R ++ +  +K+V E H YSFS D    + K   N  C +I++K     GF++
Sbjct: 248 TNLSFLRDRSVNVSFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFLL 307

Query: 310 DMDNNPYPLFLSEFGYDQSRANEAENRFMSCFLARIAAKDIDWALWAFQGSYMYRQGRED 369
                 +PL LSEFG DQ   + + NR+M+C +A  A  D+DWA+WA  G Y  R G   
Sbjct: 308 ---GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG--- 367

Query: 370 PDESFGVMDHSWTKDRNPKLRQMLQLAQRINQDPNSKAPMSYIMLHPVSGQCV---KSDG 429
                            P LR                 P   ++ HP +G CV    SD 
Sbjct: 368 -----------------PGLR-----------------PNKNLLFHPSTGLCVTNNPSDN 427

Query: 430 KGGIQLGDCTAPTTQWSHSGDASPMKLVSNGQCLKS---AGDGKPPLVSANCSADGSTWT 489
              ++LG C   +  W+   + S   L  N  C+++    G      V   CS  G    
Sbjct: 428 IPTLRLGPC-PKSDPWTF--NPSEGILWINKMCVEAPNVVGQKVKLGVGTKCSKLGQ--- 487

Query: 490 VASRAKLQLATKI-GGDNFCLEKDS-DTSIVVKKCICLKDEWNCMDDPQSQWFKLV 532
             S  K+ L+ K   G   CL+ D  D S+V  +C  L  + +C  DP SQWFK++
Sbjct: 488 -ISATKMHLSFKTSNGLLLCLDVDERDNSVVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of Lcy02g013270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	4.8e-118	40.63	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P23548	6.6e-11	20.05	Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2	[more]

BLAST of Lcy02g013270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNT6	3.2e-250	79.09	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3...	[more]
A0A5A7TIV3	6.9e-245	77.38	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3AUC3	6.9e-245	77.38	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV...	[more]
A0A6J1JQD4	4.2e-242	84.55	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=...	[more]
A0A6J1GQA8	1.3e-238	82.88	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456519 P...	[more]

BLAST of Lcy02g013270 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6601845.1	1.1e-257	81.37	hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038907081.1	4.3e-257	80.11	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
XP_004143963.2	6.6e-250	79.09	glycosyl hydrolase 5 family protein [Cucumis sativus]	[more]
XP_008437726.1	1.4e-244	77.38	PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 maj...	[more]
XP_022990540.1	8.6e-242	84.55	uncharacterized protein LOC111487386 [Cucurbita maxima]	[more]

BLAST of Lcy02g013270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26130.1	2.7e-108	41.18	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT1G13130.1	1.3e-107	39.11	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	1.9e-106	38.87	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	9.0e-96	37.17	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	4.5e-87	36.57	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 65..351 e-value: 8.5E-19 score: 68.0
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..396 e-value: 1.6E-66 score: 227.0
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 12..532
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..390
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 402..529

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g013270.1	Lcy02g013270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds