Lcy02g012840 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g012840
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGDSL esterase/lipase
LocationChr02: 38124277 .. 38126658 (+)
RNA-Seq ExpressionLcy02g012840
SyntenyLcy02g012840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACGACCCAAATTAATCGGTTCCATGTACCTCTCTTCCTGTGCAGGTCAAATGTCCCTTGCAAATTGAAATCAATGGCTTCCGATCCCGATTAACCTGCATTCGCCGACGAACGCCTTCTTCAAGCGTCGGTTCGGCAGATGCATTGCCGTCCTTAAGGTATTTCCGTGAATCCATTGCCTTCTCTCCATCGGTTTTTTCTCTTTGCAGATTCATTTCTTGGCAGGCGTCTCGTTTTTGTTGTTCTTTTGCTTCGATTCGCAATTTGCAATGTCGTGTTGATTGGTATTTCCCATTTTGATTTAGATCCGCAGTGGTTTCTGAGATGCCAATGGTTCTTTTTGTGAAGAATGATTGCGTGTTTGAGAATCAAAGTAGTTGATCTCCTTTTTCCTGGACTTCCTTCTTCTAACAGTTCTAGTTATAGCGGACTCAAGATTTTAGTTTTTCGATAGATCTTCTTGTATTGGCGTTCTGCAGTTGCTGTTCTAGACTCTGGAGCGTGAAGTTCCTCGAAATGATCTCGAAAAACAAGCAGTTCTCTTAAATGATAATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATACATATGTTAAACGAGAAATCTAGATTCAATGGCGAACTAGTTTTGGTTAATGTGTTGATTTTCTGCTTTCGTAGTTTAAATGATAAATAGTCTGGTAGTTTTCGGACAACGCCTCGTCTTTTTACTAGATGACATGAAGCTAAAGGACAAACTGTTAGTTTGAATTTTTTTTATGTTGAAATTTTGCTCGAAGGATCGCTTTCATTGAACTTCTGTAAATTGTGCTTTTTTATAAATGAACAATGAAGTTTTAATTGTTAGAGTTTATCTTTGGTGCATGATGCTACAATATATGAAACAAGTTGGATCAAGTTCTAATCCCTTTCTGAACTTTTCCTTGCTTGGTGTAGAGCAACTTTGTTTTCAAGAGGAAAGGAGAGGAATATTGAGTGTAATGGCCTCTGAGAGGGAAGTTTTTGAACTTTCAGGGCCATTACACTTGGCTCCCGTTGATTGGTAAGAATAAATTTCCCTTCTATTGACAAGAAAATAGAAGTTCGATTACTAGTATGATATGAAAAGTTTTTAGAAATTGAGTTCAGACTTCAGGGAGTCCCTTCAGTTGATTGTGGCCCAAATGCTCATTTATTTCCAGCGTTGGCAATTTCTACATTTGGGCTCTTTAATAATGTTTTTTTGGTGTTTACAATGAGTTTAAATTAGTCTGGTTGAGTAGGGGATAACATAATGGCTGCAGGGAGAATGCCGATCACAGAAGGTCAGCTGCTGCTTGTTTGGTTCAAGGTGTCTACATTTTAGAACGAGATCGCCAGGAGAAACGTCTAGGTTCTGAGGCCTTAGCTCCCCGCTGGTGGGAATTCTGCCACTTTCGGTTGCTTCGTCAGCTTGTCGATGATGTTGATTTTTCCATTTTTGGTGCTGTTTATGAATTCAAGCCTTTGCTGCTTCAAGGTGACCACAAGAATGATGGGAGTCCCCGTTATGTGATTGCCTTCCGAGGCACCCTGACCAAACCAGATTCTGTCTCTCGAGATATAGAGTTAGATCTCCACCTCATTCAAAATGGTCTGCATCGGACATCACGTTTCGAGATTGCGATGCAAGTGGTCAGGAATATGGTTGCTACTGTTGGTGATTCAAATATCTGGTTAGCTGGCCATTCCTTGGGGTCAGCCATGGCAATGCTTGCTGGAAAAACCTTGGCAAGAACAGGCGTTTTCCTTCAATCATATCTATTCAATCCACCGTTCTTCGCTGCACCGATCGAGAGAATCAAGGACAAGAAATTGAAACATGGACTACGGTTTGCTGGCAGTGTTATCACAGCAGGACTTGCTTTTGCTTTGAAGGCTAGTAGTAATAACCAGAGGAATCAATCCGAGGAACCATTTTTTGCCATTGCAGCATGGGTTCCATCTCTATTTGTTAATCCATCAGACGATCTAGGATCAGAATACATTGGATATTTTGAACACAGGAAGAAAATGGAAGATATCGGTGCAGGAAGCATCGAGAGATTAGCAACCCAGAACTCGATCGGAGGTCTTTTGATGAATGCAATCGGGAGGGAATCAGAGCCACTGCATCTCATTCCATCAGCAAACCTGATTACAAATTTAACTCCTGCACAAGACTTTAAACAAGCTCATGGCATTCACCAATGGTGGCAACCACGTATTCAATTGCAGTCAAATATCTATAAGTACTCCTAGATTTTTCCTCTTTTGAACTTGTGATAATGTAATTTATCAATGTTGTATGTATGGTGGATTAGCATTTGAGAATTGTCTCTTCTTTGTTTATAATATGAACGAGAGAACGTCTGTCCAA

mRNA sequence

AAAAAAACGACCCAAATTAATCGGTTCCATGTACCTCTCTTCCTGTGCAGGTCAAATGTCCCTTGCAAATTGAAATCAATGGCTTCCGATCCCGATTAACCTGCATTCGCCGACGAACGCCTTCTTCAAGCGTCGGTTCGGCAGATGCATTGCCGTCCTTAAGAGCAACTTTGTTTTCAAGAGGAAAGGAGAGGAATATTGAGTGTAATGGCCTCTGAGAGGGAAGTTTTTGAACTTTCAGGGCCATTACACTTGGCTCCCGTTGATTGGGAGAATGCCGATCACAGAAGGTCAGCTGCTGCTTGTTTGGTTCAAGGTGTCTACATTTTAGAACGAGATCGCCAGGAGAAACGTCTAGGTTCTGAGGCCTTAGCTCCCCGCTGGTGGGAATTCTGCCACTTTCGGTTGCTTCGTCAGCTTGTCGATGATGTTGATTTTTCCATTTTTGGTGCTGTTTATGAATTCAAGCCTTTGCTGCTTCAAGGTGACCACAAGAATGATGGGAGTCCCCGTTATGTGATTGCCTTCCGAGGCACCCTGACCAAACCAGATTCTGTCTCTCGAGATATAGAGTTAGATCTCCACCTCATTCAAAATGGTCTGCATCGGACATCACGTTTCGAGATTGCGATGCAAGTGGTCAGGAATATGGTTGCTACTGTTGGTGATTCAAATATCTGGTTAGCTGGCCATTCCTTGGGGTCAGCCATGGCAATGCTTGCTGGAAAAACCTTGGCAAGAACAGGCGTTTTCCTTCAATCATATCTATTCAATCCACCGTTCTTCGCTGCACCGATCGAGAGAATCAAGGACAAGAAATTGAAACATGGACTACGGTTTGCTGGCAGTGTTATCACAGCAGGACTTGCTTTTGCTTTGAAGGCTAGTAGTAATAACCAGAGGAATCAATCCGAGGAACCATTTTTTGCCATTGCAGCATGGGTTCCATCTCTATTTGTTAATCCATCAGACGATCTAGGATCAGAATACATTGGATATTTTGAACACAGGAAGAAAATGGAAGATATCGGTGCAGGAAGCATCGAGAGATTAGCAACCCAGAACTCGATCGGAGGTCTTTTGATGAATGCAATCGGGAGGGAATCAGAGCCACTGCATCTCATTCCATCAGCAAACCTGATTACAAATTTAACTCCTGCACAAGACTTTAAACAAGCTCATGGCATTCACCAATGGTGGCAACCACGTATTCAATTGCAGTCAAATATCTATAAGTACTCCTAGATTTTTCCTCTTTTGAACTTGTGATAATGTAATTTATCAATGTTGTATGTATGGTGGATTAGCATTTGAGAATTGTCTCTTCTTTGTTTATAATATGAACGAGAGAACGTCTGTCCAA

Coding sequence (CDS)

ATGGCCTCTGAGAGGGAAGTTTTTGAACTTTCAGGGCCATTACACTTGGCTCCCGTTGATTGGGAGAATGCCGATCACAGAAGGTCAGCTGCTGCTTGTTTGGTTCAAGGTGTCTACATTTTAGAACGAGATCGCCAGGAGAAACGTCTAGGTTCTGAGGCCTTAGCTCCCCGCTGGTGGGAATTCTGCCACTTTCGGTTGCTTCGTCAGCTTGTCGATGATGTTGATTTTTCCATTTTTGGTGCTGTTTATGAATTCAAGCCTTTGCTGCTTCAAGGTGACCACAAGAATGATGGGAGTCCCCGTTATGTGATTGCCTTCCGAGGCACCCTGACCAAACCAGATTCTGTCTCTCGAGATATAGAGTTAGATCTCCACCTCATTCAAAATGGTCTGCATCGGACATCACGTTTCGAGATTGCGATGCAAGTGGTCAGGAATATGGTTGCTACTGTTGGTGATTCAAATATCTGGTTAGCTGGCCATTCCTTGGGGTCAGCCATGGCAATGCTTGCTGGAAAAACCTTGGCAAGAACAGGCGTTTTCCTTCAATCATATCTATTCAATCCACCGTTCTTCGCTGCACCGATCGAGAGAATCAAGGACAAGAAATTGAAACATGGACTACGGTTTGCTGGCAGTGTTATCACAGCAGGACTTGCTTTTGCTTTGAAGGCTAGTAGTAATAACCAGAGGAATCAATCCGAGGAACCATTTTTTGCCATTGCAGCATGGGTTCCATCTCTATTTGTTAATCCATCAGACGATCTAGGATCAGAATACATTGGATATTTTGAACACAGGAAGAAAATGGAAGATATCGGTGCAGGAAGCATCGAGAGATTAGCAACCCAGAACTCGATCGGAGGTCTTTTGATGAATGCAATCGGGAGGGAATCAGAGCCACTGCATCTCATTCCATCAGCAAACCTGATTACAAATTTAACTCCTGCACAAGACTTTAAACAAGCTCATGGCATTCACCAATGGTGGCAACCACGTATTCAATTGCAGTCAAATATCTATAAGTACTCCTAG

Protein sequence

MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWWEFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTGVFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS
Homology
BLAST of Lcy02g012840 vs. ExPASy Swiss-Prot
Match: Q680C0 (GDSL esterase/lipase At4g10955 OS=Arabidopsis thaliana OX=3702 GN=At4g10955 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.1e-119
Identity = 206/352 (58.52%), Postives = 267/352 (75.85%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSE-ALAPRW 60
           M SER+ F L+GPLHL  +DW N  HRRS A  LVQG+Y+ ERDRQ +R G E AL+P W
Sbjct: 2   MISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPIW 61

Query: 61  WEFCHFRLLRQLVDDVDFSIFGAVYEFK--PLLLQGDHKNDGSPRYVIAFRGTLTKPDSV 120
            EF HFRL+R+ VDD D SIFG +YE+K    L Q     + SPR+VIAFRGT+TK DS+
Sbjct: 62  SEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDSI 121

Query: 121 SRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLA 180
           SRDIE D+H+I+NGLH T+RFEIA+Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+A
Sbjct: 122 SRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTIA 181

Query: 181 RTGVFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQ--- 240
           RTG F + + FNPPF +APIE+IKDK++KHG+R AGSVITAGLA A KA+ +  +N    
Sbjct: 182 RTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRAL 241

Query: 241 --SEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLL 300
               +PF A++ W P L+VNP D L SEY+GYFEHR KME+IG G +ER+ATQ+S+GG+L
Sbjct: 242 PAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGML 301

Query: 301 MNAIGRESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKY 345
           +       EP+HLIPS+ L  NL+ ++DFKQAHGIHQWW+   + ++ +Y+Y
Sbjct: 302 LGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Lcy02g012840 vs. ExPASy TrEMBL
Match: A0A6J1KA98 (GDSL esterase/lipase At4g10955 OS=Cucurbita maxima OX=3661 GN=LOC111491521 PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 8.0e-186
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Lcy02g012840 vs. ExPASy TrEMBL
Match: A0A6J1H0Q1 (GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459360 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Lcy02g012840 vs. ExPASy TrEMBL
Match: A0A6J1H0S6 (GDSL esterase/lipase At4g10955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459360 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 22  MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 81

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 82  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 141

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 142 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 201

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 202 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 261

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 262 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 321

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 322 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 366

BLAST of Lcy02g012840 vs. ExPASy TrEMBL
Match: A0A6J1DX98 (GDSL esterase/lipase At4g10955 OS=Momordica charantia OX=3673 GN=LOC111025331 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 311/345 (90.14%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PVDWENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVDWENADHRRSVAACLVQGVYILERDRQEKRQGSDALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRL RQLVDDVD SIFGA+YEFKP+LLQGDHK DGSPRYVIAFRGTLT+PDSVSRD
Sbjct: 61  EFCHFRLFRQLVDDVDSSIFGAIYEFKPVLLQGDHKIDGSPRYVIAFRGTLTRPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH +QNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKT+ARTG
Sbjct: 121 IELDLHFMQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFFAAPIERIKDKKLKHG+R AGSVITAGLAFALKAS+NNQRN++EEPFF
Sbjct: 181 IFLESYLFNPPFFAAPIERIKDKKLKHGIRIAGSVITAGLAFALKASNNNQRNRTEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AIAAWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLATQNSIGGLL+NAIGRES
Sbjct: 241 AIAAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATQNSIGGLLLNAIGRES 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSA+L  NL PAQ+FKQAHGIHQWWQP +QL S IYKYS
Sbjct: 301 EPLHLIPSASLTVNLAPAQEFKQAHGIHQWWQPHLQLHSKIYKYS 345

BLAST of Lcy02g012840 vs. ExPASy TrEMBL
Match: A0A1S3AUJ1 (GDSL esterase/lipase At4g10955 OS=Cucumis melo OX=3656 GN=LOC103482838 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.6e-181
Identity = 311/346 (89.88%), Postives = 328/346 (94.80%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHLA VDWENA+HRRS AACLVQGVYILERDRQEKR G+EA APRWW
Sbjct: 1   MASEREVFELSGPLHLASVDWENANHRRSVAACLVQGVYILERDRQEKRQGTEAFAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLRQLVDDVD SIFGAVYEFKPLLLQG HK DGSPR+VIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRQLVDDVDSSIFGAVYEFKPLLLQGHHKVDGSPRFVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAMLAG+T+ARTG
Sbjct: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNVWLAGHSLGSAMAMLAGRTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKA-SSNNQRNQSEEPF 240
           +FL+SYLFNPPFFAAPIERIKDKKLKHGLR AGSVITAGLA ALKA S+NNQRN  EEPF
Sbjct: 181 IFLKSYLFNPPFFAAPIERIKDKKLKHGLRIAGSVITAGLALALKAGSNNNQRNGIEEPF 240

Query: 241 FAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRE 300
           FAIAAWVPSLFVNPSD +GSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLL+NA+GRE
Sbjct: 241 FAIAAWVPSLFVNPSDHVGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLLNALGRE 300

Query: 301 SEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           SEP+HLIPSA L+ NL+PAQDFKQAHGIHQWWQP +QL S IY+YS
Sbjct: 301 SEPIHLIPSAELVVNLSPAQDFKQAHGIHQWWQPHLQLHSKIYEYS 346

BLAST of Lcy02g012840 vs. NCBI nr
Match: XP_022996235.1 (GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996236.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996237.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996238.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996239.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_023534452.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534453.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534455.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534456.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534457.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 1.6e-185
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Lcy02g012840 vs. NCBI nr
Match: XP_022957996.1 (GDSL esterase/lipase At4g10955 isoform X1 [Cucurbita moschata])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 22  MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 81

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 82  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 141

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 142 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 201

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 202 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 261

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 262 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 321

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 322 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 366

BLAST of Lcy02g012840 vs. NCBI nr
Match: XP_022957997.1 (GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022957998.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022957999.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022958000.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 313/345 (90.72%), Postives = 331/345 (95.94%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Lcy02g012840 vs. NCBI nr
Match: KAG6605591.1 (hypothetical protein SDJN03_02908, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655.2 bits (1689), Expect = 3.1e-184
Identity = 311/343 (90.67%), Postives = 329/343 (95.92%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PV+WENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRLLR LVDDVD SIFGAVYEFKP+LLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH IQNGLHRTSRFEIAMQVVRNMVATVGDSN+WLAGHSLGSAMAML GKT+ARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFF+APIERIKDKKLKHG+R AGSVITAGLAFALKASSNNQRN  EEPFF
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AI+AWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLAT+NSIGGLLMNAIGR+S
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYK 344
           EPLHLIPSANLITNL+P QDFKQAHGIHQWWQP ++LQSNIY+
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYR 343

BLAST of Lcy02g012840 vs. NCBI nr
Match: XP_022158868.1 (GDSL esterase/lipase At4g10955 [Momordica charantia])

HSP 1 Score: 654.8 bits (1688), Expect = 4.0e-184
Identity = 311/345 (90.14%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MASEREVFELSGPLHL PVDWENADHRRS AACLVQGVYILERDRQEKR GS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVDWENADHRRSVAACLVQGVYILERDRQEKRQGSDALAPRWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           EFCHFRL RQLVDDVD SIFGA+YEFKP+LLQGDHK DGSPRYVIAFRGTLT+PDSVSRD
Sbjct: 61  EFCHFRLFRQLVDDVDSSIFGAIYEFKPVLLQGDHKIDGSPRYVIAFRGTLTRPDSVSRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELDLH +QNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKT+ARTG
Sbjct: 121 IELDLHFMQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTMARTG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSEEPFF 240
           +FL+SYLFNPPFFAAPIERIKDKKLKHG+R AGSVITAGLAFALKAS+NNQRN++EEPFF
Sbjct: 181 IFLESYLFNPPFFAAPIERIKDKKLKHGIRIAGSVITAGLAFALKASNNNQRNRTEEPFF 240

Query: 241 AIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAIGRES 300
           AIAAWVPSLFVNPSD +GSEYIGYFEHRK ME+IGAGSIERLATQNSIGGLL+NAIGRES
Sbjct: 241 AIAAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATQNSIGGLLLNAIGRES 300

Query: 301 EPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           EPLHLIPSA+L  NL PAQ+FKQAHGIHQWWQP +QL S IYKYS
Sbjct: 301 EPLHLIPSASLTVNLAPAQEFKQAHGIHQWWQPHLQLHSKIYKYS 345

BLAST of Lcy02g012840 vs. TAIR 10
Match: AT2G05260.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 6.1e-122
Identity = 211/359 (58.77%), Postives = 274/359 (76.32%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MAS+RE F L GP HL  VDW N DH+R  AACLVQG+YI+E+DRQ KR G+EALA  WW
Sbjct: 1   MASDREEFNLCGPTHLTTVDWGNEDHQRCVAACLVQGIYIVEQDRQLKREGTEALASPWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           E  +F+L+R L DD DFSIFG ++E+K   LQ D  + G PRYVIAFRGTLTK DS++RD
Sbjct: 61  ESFNFKLIRHLKDDADFSIFGGIFEYKS--LQPDVVDSGVPRYVIAFRGTLTKADSITRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELD+H+I+NGLHRTSRFEIAMQ VR+M  +VG S+ WL GHSLG+AMA+LAGKT+ ++G
Sbjct: 121 IELDIHIIRNGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGKTMGKSG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFA--LKASSNNQRNQ---- 240
           V+++S LFNPP+ + PIERI +++++HG+RFAGS+ITAGLA +  LK +   Q+ Q    
Sbjct: 181 VYIKSLLFNPPYVSPPIERIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQQQQLQLQ 240

Query: 241 --SEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDI--GAGSIERLATQNSIGG 300
             +E+P  A+++W+P++ VNP D L SEYIG+FEHR  ME I  GAG +ER+A Q+S+GG
Sbjct: 241 NLTEDPLEALSSWLPNIHVNPGDHLCSEYIGFFEHRGNMEQIGYGAGIVERMAMQHSLGG 300

Query: 301 LLMNAIG-----RESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKY 345
           LLM+A+G        EP+H+IPSANLI N T ++D+K AHGIHQWW+    L S+IY Y
Sbjct: 301 LLMDAMGVSNAVEVEEPVHVIPSANLIVNKTISEDYKDAHGIHQWWRDDQDLVSHIYMY 357

BLAST of Lcy02g012840 vs. TAIR 10
Match: AT4G10955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.5e-120
Identity = 206/352 (58.52%), Postives = 267/352 (75.85%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSE-ALAPRW 60
           M SER+ F L+GPLHL  +DW N  HRRS A  LVQG+Y+ ERDRQ +R G E AL+P W
Sbjct: 2   MISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPIW 61

Query: 61  WEFCHFRLLRQLVDDVDFSIFGAVYEFK--PLLLQGDHKNDGSPRYVIAFRGTLTKPDSV 120
            EF HFRL+R+ VDD D SIFG +YE+K    L Q     + SPR+VIAFRGT+TK DS+
Sbjct: 62  SEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDSI 121

Query: 121 SRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLA 180
           SRDIE D+H+I+NGLH T+RFEIA+Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+A
Sbjct: 122 SRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTIA 181

Query: 181 RTGVFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQ--- 240
           RTG F + + FNPPF +APIE+IKDK++KHG+R AGSVITAGLA A KA+ +  +N    
Sbjct: 182 RTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRAL 241

Query: 241 --SEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLL 300
               +PF A++ W P L+VNP D L SEY+GYFEHR KME+IG G +ER+ATQ+S+GG+L
Sbjct: 242 PAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGML 301

Query: 301 MNAIGRESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKY 345
           +       EP+HLIPS+ L  NL+ ++DFKQAHGIHQWW+   + ++ +Y+Y
Sbjct: 302 LGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Lcy02g012840 vs. TAIR 10
Match: AT4G10955.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.5e-120
Identity = 206/352 (58.52%), Postives = 267/352 (75.85%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSE-ALAPRW 60
           M SER+ F L+GPLHL  +DW N  HRRS A  LVQG+Y+ ERDRQ +R G E AL+P W
Sbjct: 2   MISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPIW 61

Query: 61  WEFCHFRLLRQLVDDVDFSIFGAVYEFK--PLLLQGDHKNDGSPRYVIAFRGTLTKPDSV 120
            EF HFRL+R+ VDD D SIFG +YE+K    L Q     + SPR+VIAFRGT+TK DS+
Sbjct: 62  SEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDSI 121

Query: 121 SRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLA 180
           SRDIE D+H+I+NGLH T+RFEIA+Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+A
Sbjct: 122 SRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTIA 181

Query: 181 RTGVFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQ--- 240
           RTG F + + FNPPF +APIE+IKDK++KHG+R AGSVITAGLA A KA+ +  +N    
Sbjct: 182 RTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRAL 241

Query: 241 --SEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLL 300
               +PF A++ W P L+VNP D L SEY+GYFEHR KME+IG G +ER+ATQ+S+GG+L
Sbjct: 242 PAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGML 301

Query: 301 MNAIGRESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKY 345
           +       EP+HLIPS+ L  NL+ ++DFKQAHGIHQWW+   + ++ +Y+Y
Sbjct: 302 LGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Lcy02g012840 vs. TAIR 10
Match: AT5G24230.1 (Lipase class 3-related protein )

HSP 1 Score: 382.9 bits (982), Expect = 2.8e-106
Identity = 181/355 (50.99%), Postives = 254/355 (71.55%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MAS+RE+F +SGP+HL  +DW N+ HR S A+CLVQ VY LERDRQ+ R+G ++ A  WW
Sbjct: 15  MASQREIFSISGPIHLTSIDWNNSYHRTSVASCLVQAVYTLERDRQQNRIGLKSQANHWW 74

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGS---PRYVIAFRGTLTKPDSV 120
           EF +F L   L+DD D SI+GAV+E+K       H    S   PR+VIAFRGT+ KP S 
Sbjct: 75  EFFNFTLAETLIDDSDGSIYGAVFEYKHFFSYNYHHTPHSKPPPRHVIAFRGTILKPHSR 134

Query: 121 SRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLA 180
           SRD++LDL  I++ LH ++RF  A+QV+++ VA  G++ +WLAGHSLG+A+A+LAGK + 
Sbjct: 135 SRDLKLDLRCIRDSLHDSTRFVHAIQVIQSAVAKTGNAAVWLAGHSLGAAVALLAGKIMT 194

Query: 181 RTGVFLQSYLFNPPFFAAPIER-IKDKKLKHGLRFAGSVITAGLAFALKASSNNQRNQSE 240
           R+G  L+SYLFNPPF + PIE+ +K +KLKHG+RFAGS++ AG+A A+K   +N + Q +
Sbjct: 195 RSGFPLESYLFNPPFSSIPIEKLVKSEKLKHGVRFAGSLVKAGVAIAVKGRHHN-KGQED 254

Query: 241 EPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGSIERLATQNSIGGLLMNAI 300
           + F  +A+W+P L++NP D + SEYIGYF+HR KM +IGAG IER+AT+NS+  LL    
Sbjct: 255 DSFMKLASWIPYLYLNPLDTICSEYIGYFKHRNKMFEIGAGKIERIATRNSLRSLLSGGG 314

Query: 301 G------RESEPLHLIPSANLITNLTPAQDFKQAHGIHQWWQPRIQLQSNIYKYS 346
           G        SEPLHL+PSA +  N + + +FK+AHGIHQWW P    +  +++++
Sbjct: 315 GGGSSSDSSSEPLHLLPSAYMTINASKSPNFKRAHGIHQWWDPMFNGEYVLHQFN 368

BLAST of Lcy02g012840 vs. TAIR 10
Match: AT2G05260.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 360.5 bits (924), Expect = 1.5e-99
Identity = 172/286 (60.14%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 1   MASEREVFELSGPLHLAPVDWENADHRRSAAACLVQGVYILERDRQEKRLGSEALAPRWW 60
           MAS+RE F L GP HL  VDW N DH+R  AACLVQG+YI+E+DRQ KR G+EALA  WW
Sbjct: 1   MASDREEFNLCGPTHLTTVDWGNEDHQRCVAACLVQGIYIVEQDRQLKREGTEALASPWW 60

Query: 61  EFCHFRLLRQLVDDVDFSIFGAVYEFKPLLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120
           E  +F+L+R L DD DFSIFG ++E+K   LQ D  + G PRYVIAFRGTLTK DS++RD
Sbjct: 61  ESFNFKLIRHLKDDADFSIFGGIFEYKS--LQPDVVDSGVPRYVIAFRGTLTKADSITRD 120

Query: 121 IELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTLARTG 180
           IELD+H+I+NGLHRTSRFEIAMQ VR+M  +VG S+ WL GHSLG+AMA+LAGKT+ ++G
Sbjct: 121 IELDIHIIRNGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGKTMGKSG 180

Query: 181 VFLQSYLFNPPFFAAPIERIKDKKLKHGLRFAGSVITAGLAFA--LKASSNNQRNQ---- 240
           V+++S LFNPP+ + PIERI +++++HG+RFAGS+ITAGLA +  LK +   Q+ Q    
Sbjct: 181 VYIKSLLFNPPYVSPPIERIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQQQQLQLQ 240

Query: 241 --SEEPFFAIAAWVPSLFVNPSDDLGSEYIGYFEHRKKMEDIGAGS 279
             +E+P  A+++W+P++ VNP D L SEYIG+FEHR  ME IG GS
Sbjct: 241 NLTEDPLEALSSWLPNIHVNPGDHLCSEYIGFFEHRGNMEQIGYGS 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q680C02.1e-11958.52GDSL esterase/lipase At4g10955 OS=Arabidopsis thaliana OX=3702 GN=At4g10955 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1KA988.0e-18690.72GDSL esterase/lipase At4g10955 OS=Cucurbita maxima OX=3661 GN=LOC111491521 PE=4 ... [more]
A0A6J1H0Q11.0e-18590.72GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1H0S61.0e-18590.72GDSL esterase/lipase At4g10955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1DX982.0e-18490.14GDSL esterase/lipase At4g10955 OS=Momordica charantia OX=3673 GN=LOC111025331 PE... [more]
A0A1S3AUJ11.6e-18189.88GDSL esterase/lipase At4g10955 OS=Cucumis melo OX=3656 GN=LOC103482838 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022996235.11.6e-18590.72GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996236.1 GDSL esterase/... [more]
XP_022957996.12.1e-18590.72GDSL esterase/lipase At4g10955 isoform X1 [Cucurbita moschata][more]
XP_022957997.12.1e-18590.72GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022957998.1 G... [more]
KAG6605591.13.1e-18490.67hypothetical protein SDJN03_02908, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022158868.14.0e-18490.14GDSL esterase/lipase At4g10955 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G05260.16.1e-12258.77alpha/beta-Hydrolases superfamily protein [more]
AT4G10955.11.5e-12058.52alpha/beta-Hydrolases superfamily protein [more]
AT4G10955.21.5e-12058.52alpha/beta-Hydrolases superfamily protein [more]
AT5G24230.12.8e-10650.99Lipase class 3-related protein [more]
AT2G05260.21.5e-9960.14alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 130..182
e-value: 1.3E-6
score: 28.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 79..207
e-value: 9.5E-10
score: 40.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 32..195
NoneNo IPR availablePANTHERPTHR31479:SF19LIPASE CLASS 3-LIKEcoord: 2..345
NoneNo IPR availablePANTHERPTHR31479ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 2..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g012840.1Lcy02g012840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process