Lcy02g007220 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g007220 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.8e-13
Identity = 53/192 (27.60%), Postives = 96/192 (50.00%), Query Frame = 0

Query: 141 FPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCFLLLIRGTHSIKDTLTAVTGA 200
           F   L++ G    D +       + +  F V+ D   +  ++ +RGT S++D LT ++  
Sbjct: 330 FASILKTTGLQYRDFIHVSFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE 389

Query: 201 VVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAK--LSTPFLLKGLDDFPDYKIKIVGH 260
                 ++  D  + + V   AH G+  AAR+I +  ++   L +     P+Y++ +VGH
Sbjct: 390 ----SENLELDIELQDCV---AHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGH 449

Query: 261 SLGGGTAALLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSF 320
           SLG G AALL  +LR    +       F+ P   ++  L E  K F+ ++I G D++P  
Sbjct: 450 SLGAGAAALLAIMLR--GAYPQVRAYAFSPPRGLLSKSLFEYSKDFVVSLILGMDVIPRL 509

Query: 321 SAASIDDLRYEV 330
           S A+++DL+  +
Sbjct: 510 SVANMEDLKRRI 512

BLAST of Lcy02g007220 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 56/188 (29.79%), Postives = 88/188 (46.81%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTVIRDSNSKCFLLLIRGTHSIKDTLTAVTGAV----VPFHHSVL 213
           D++       + +  F V  D + K  ++ IRGT S KD LT +TG      V  HH   
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGT- 426

Query: 214 HDGGISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKGLDDFPDYKIKIVGHSLGG 273
                        H GMV +A +I K       LS  F          Y + +VGHSLG 
Sbjct: 427 ----------WLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGA 486

Query: 274 GTAALLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAAS 330
           GTAA+L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + 
Sbjct: 487 GTAAILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQ 541

BLAST of Lcy02g007220 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 1.4e-12
Identity = 57/184 (30.98%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTVIRDSNSKCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGG 213
           D++       + +  F V  D + K  ++ IRGT S KD LT +TG         L   G
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDA-----ERLPVEG 426

Query: 214 ISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKGLDDFPDYKIKIVGHSLGGGTAA 273
                LG  H GMV +A +I K       LS  F          Y + +VGHSLG GTAA
Sbjct: 427 HRGTWLG--HKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAA 486

Query: 274 LLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAASIDDL 330
           +L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + ++  
Sbjct: 487 ILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGF 541

BLAST of Lcy02g007220 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.4e-12
Identity = 57/184 (30.98%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 154 DVLIQKPKAGILKPAFTVIRDSNSKCFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGG 213
           D++       + +  F V  D + K  ++ IRGT S KD LT +TG         L   G
Sbjct: 367 DIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDA-----ERLPVEG 426

Query: 214 ISNLVLGYAHFGMVAAARWIAK-------LSTPFLLKGLDDFPDYKIKIVGHSLGGGTAA 273
                LG  H GMV +A +I K       LS  F          Y + +VGHSLG GTAA
Sbjct: 427 HRGTWLG--HKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAA 486

Query: 274 LLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSFSAASIDDL 330
           +L+++LR Q  + +  C  ++ P   ++ +  E  K+F+T ++ G DLVP    + ++  
Sbjct: 487 ILSFLLRPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGF 541

BLAST of Lcy02g007220 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 3.2e-12
Identity = 54/192 (28.12%), Postives = 94/192 (48.96%), Query Frame = 0

Query: 141 FPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCFLLLIRGTHSIKDTLTAVTGA 200
           F   L++ G    D +       + +  F V+ D   +  ++ +RGT S++D LT ++  
Sbjct: 330 FASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSA- 389

Query: 201 VVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAK--LSTPFLLKGLDDFPDYKIKIVGH 260
                 S   + GI  L    AH G+  AAR+I +  ++   L +     P+Y++ +VGH
Sbjct: 390 -----ESETLELGI-ELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGH 449

Query: 261 SLGGGTAALLTYILREQKEFSSSTCITFA-PAACMTWELAESGKQFITTIINGSDLVPSF 320
           SLG G AALL  +LR    +       F+ P   ++  L E  K F+ ++I G D++P  
Sbjct: 450 SLGAGAAALLAIMLR--GAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRL 509

Query: 321 SAASIDDLRYEV 330
           S  +++DL+  +
Sbjct: 510 SVTNMEDLKRRI 512

BLAST of Lcy02g007220 vs. ExPASy TrEMBL
Match: A0A5D3BT55 (Sn1-specific diacylglycerol lipase alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00010 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 599/640 (93.59%), Postives = 618/640 (96.56%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQE+VLIQKPKAGILKPAFT+IRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGG+LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSS-DDSSDHDTDDERHHLIHEE 480
           TEELPE PRITER HESLI+E+ TIN IEKKKK ESGSSS DD+SDHDTD+ERHHLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVE 540
           RI++STDVEDITDGELWYELEKELQRQE+KVD   TRE  VA  AKEIKEEEE MLTDVE
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVD-ANTREGKVATVAKEIKEEEESMLTDVE 540

Query: 541 GSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPREL 600
           GSSEKPLSSLDASEN+RFYPPGKTMHIVS PSP+SDNLVQDD+DE +QE VGIYETPREL
Sbjct: 541 GSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETPREL 600

Query: 601 YSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           YSKLRLSRTMINDHYMPMYKKMME LIN+LE DV S+YEM
Sbjct: 601 YSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of Lcy02g007220 vs. ExPASy TrEMBL
Match: A0A1S3C7B1 (uncharacterized protein LOC103497826 OS=Cucumis melo OX=3656 GN=LOC103497826 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 599/640 (93.59%), Postives = 618/640 (96.56%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQE+VLIQKPKAGILKPAFT+IRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGG+LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSS-DDSSDHDTDDERHHLIHEE 480
           TEELPE PRITER HESLI+E+ TIN IEKKKK ESGSSS DD+SDHDTD+ERHHLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVE 540
           RI++STDVEDITDGELWYELEKELQRQE+KVD   TRE  VA  AKEIKEEEE MLTDVE
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVD-ANTREGKVATVAKEIKEEEESMLTDVE 540

Query: 541 GSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPREL 600
           GSSEKPLSSLDASEN+RFYPPGKTMHIVS PSP+SDNLVQDD+DE +QE VGIYETPREL
Sbjct: 541 GSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETPREL 600

Query: 601 YSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           YSKLRLSRTMINDHYMPMYKKMME LIN+LE DV S+YEM
Sbjct: 601 YSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of Lcy02g007220 vs. ExPASy TrEMBL
Match: A0A0A0KI10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G314110 PE=4 SV=1)

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 589/639 (92.18%), Postives = 607/639 (94.99%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYY LSRRLAAKGDEDDRSGNLSKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYFLSRRLAAKGDEDDRSGNLSKSIRSCRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQE+VLIQKPKAGILKPAFT+IRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQ EFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQTEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNG +LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGNILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDERHHLIHEER 480
           TEELPE P +TER HESL  E+  IN IEKKKK E GSS DDSSDHDTD+E+HH+I  ER
Sbjct: 421 TEELPEVPLMTERNHESLKCEEVRINGIEKKKKPEFGSSCDDSSDHDTDEEQHHIITGER 480

Query: 481 IVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVEG 540
           I++STDVEDITDGELWYELEKELQRQEKKVD   TREA VA   KEIKEEEE MLTDVEG
Sbjct: 481 IIASTDVEDITDGELWYELEKELQRQEKKVD-ANTREAKVATVGKEIKEEEESMLTDVEG 540

Query: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPRELY 600
           SSEKPLSSLDASENIRFYPPGKTMHIVS PSP+SDNLVQDD+DE  QE VGIYETPRELY
Sbjct: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSTPSPNSDNLVQDDEDESTQEIVGIYETPRELY 600

Query: 601 SKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           SKLRLSRTMINDHYMPMYKKMME LIN+LE+DV S+YEM
Sbjct: 601 SKLRLSRTMINDHYMPMYKKMMESLINQLEKDVISNYEM 638

BLAST of Lcy02g007220 vs. ExPASy TrEMBL
Match: A0A6J1H5B8 (uncharacterized protein LOC111460272 OS=Cucurbita moschata OX=3662 GN=LOC111460272 PE=4 SV=1)

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 586/649 (90.29%), Postives = 613/649 (94.45%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLAAKGDEDDRS NLS+SIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFT+IRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRE+K+FSS+TCI FAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRERKDFSSTTCIAFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQ AVKSAVVRTRSSL+SWSCMGARRRNGGLL NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 TEELPEAPRITERKHESLIAED--STINTIEKKKKLESGSSS---DDSSDHDTDDERHHL 480
           TEELPEAP  TERKHESLIA+D  +T NTI KKKKLESGSSS   DDSSDHDTDDERHHL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTILKKKKLESGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVSSTDVEDITDGELWYELEKELQRQEKKVDV---VITREADVAAAAKEIKEEE 540
           I+E++ + SSTDV+DITDGELWYELEKELQRQEKK      VITREA+VAA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTKTTTDVITREANVAAVAKEIVEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVG 600
           E MLT+VEGSSEKPLSSLDASENIRFYPPGKTMHIVS+PS DSDN     D+EIVQERVG
Sbjct: 541 ESMLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFV-HDNEIVQERVG 600

Query: 601 IYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSS-YEM 640
           IYETPR LY KLRLSRTMINDHYMPMYKKM+ELLIN+LERDVSSS YEM
Sbjct: 601 IYETPRGLYGKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of Lcy02g007220 vs. ExPASy TrEMBL
Match: A0A6J1KUU3 (uncharacterized protein LOC111498916 OS=Cucurbita maxima OX=3661 GN=LOC111498916 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 586/649 (90.29%), Postives = 612/649 (94.30%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLAAKGDEDDRS NLS+SIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFT+IRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQK+FSS+TCI FAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKDFSSTTCIAFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQ AVKSAVVRTRSSL+SWSCMGARRRNGGLL NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 TEELPEAPRITERKHESLIAED--STINTIEKKKKLESGSSS---DDSSDHDTDDERHHL 480
           TEELPEAP  TERKHESLIA+D  +T NTI KKKKLE GSSS   DDSSDHDTDDERHHL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTIVKKKKLEFGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVSSTDVEDITDGELWYELEKELQRQEKKVDV---VITREADVAAAAKEIKEEE 540
           I+E++ + SSTDV+DITDGELWYELEKELQRQEKK      VITREA+VAA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTRTTTDVITREANVAAVAKEIVEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVG 600
           E MLT+VEGSSEKPLSSLDASENIRFYPPGKTMHIVS+PS DSDN     D+EIVQERVG
Sbjct: 541 ESMLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFV-HDNEIVQERVG 600

Query: 601 IYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSS-YEM 640
           IYETPR LY KLRLSRTMINDHYMPMYKKM+ELLIN+LERDVSSS YEM
Sbjct: 601 IYETPRGLYGKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of Lcy02g007220 vs. NCBI nr
Match: XP_038874736.1 (uncharacterized protein LOC120067274 [Benincasa hispida])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 601/639 (94.05%), Postives = 617/639 (96.56%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRS NLSKSIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSDNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
            EL+SFLRLLTFCMLFSKKPFPIFLESAGYS E+VLIQKPKAGILKPAFT+IRDSNSKCF
Sbjct: 121 TELKSFLRLLTFCMLFSKKPFPIFLESAGYSPEEVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGG+LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDERHHLIHEER 480
           TEELPE P ITER HESL+A + TIN IEKKKK ESG+S DDSSD DTD+ERHHLI EER
Sbjct: 421 TEELPEVPLITERNHESLLAGEVTINAIEKKKKPESGTSGDDSSDRDTDEERHHLITEER 480

Query: 481 IVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVEG 540
           +++STDVEDITDGELWYELEKELQRQEKKVDV  TREADVA  AKEIKEEEE MLTDVEG
Sbjct: 481 MIASTDVEDITDGELWYELEKELQRQEKKVDVT-TREADVATVAKEIKEEEESMLTDVEG 540

Query: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPRELY 600
           SSEKPLSSLDASENIRFYPPGKTMHIVSIPS DSDNLVQDDD+EI+QERVGIYETPRELY
Sbjct: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSIPSSDSDNLVQDDDEEIMQERVGIYETPRELY 600

Query: 601 SKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           SKLRLSRTMINDHYMPMYKKMME LINELE+DV S+YEM
Sbjct: 601 SKLRLSRTMINDHYMPMYKKMMESLINELEKDVLSNYEM 638

BLAST of Lcy02g007220 vs. NCBI nr
Match: XP_008458404.1 (PREDICTED: uncharacterized protein LOC103497826 [Cucumis melo] >TYK02921.1 Sn1-specific diacylglycerol lipase alpha [Cucumis melo var. makuwa])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 599/640 (93.59%), Postives = 618/640 (96.56%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQE+VLIQKPKAGILKPAFT+IRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGG+LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSS-DDSSDHDTDDERHHLIHEE 480
           TEELPE PRITER HESLI+E+ TIN IEKKKK ESGSSS DD+SDHDTD+ERHHLI EE
Sbjct: 421 TEELPEVPRITERNHESLISEEVTINGIEKKKKTESGSSSCDDNSDHDTDEERHHLITEE 480

Query: 481 RIVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVE 540
           RI++STDVEDITDGELWYELEKELQRQE+KVD   TRE  VA  AKEIKEEEE MLTDVE
Sbjct: 481 RIIASTDVEDITDGELWYELEKELQRQERKVD-ANTREGKVATVAKEIKEEEESMLTDVE 540

Query: 541 GSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPREL 600
           GSSEKPLSSLDASEN+RFYPPGKTMHIVS PSP+SDNLVQDD+DE +QE VGIYETPREL
Sbjct: 541 GSSEKPLSSLDASENVRFYPPGKTMHIVSTPSPNSDNLVQDDEDESMQEIVGIYETPREL 600

Query: 601 YSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           YSKLRLSRTMINDHYMPMYKKMME LIN+LE DV S+YEM
Sbjct: 601 YSKLRLSRTMINDHYMPMYKKMMESLINQLENDVISNYEM 639

BLAST of Lcy02g007220 vs. NCBI nr
Match: XP_004150086.1 (uncharacterized protein LOC101210872 [Cucumis sativus] >KGN47411.1 hypothetical protein Csa_022986 [Cucumis sativus])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 589/639 (92.18%), Postives = 607/639 (94.99%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGAMATYAGAALIIYY LSRRLAAKGDEDDRSGNLSKSIRS RRRISRRPAQAPATWF
Sbjct: 1   MAAGAMATYAGAALIIYYFLSRRLAAKGDEDDRSGNLSKSIRSCRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQLKGPEII
Sbjct: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQE+VLIQKPKAGILKPAFT+IRDS+SKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEEVLIQKPKAGILKPAFTIIRDSSSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQ EFSSSTCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQTEFSSSTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIINGSDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVLNVVYRSASALGS
Sbjct: 301 KQFITTIINGSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNG +LSNP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGNILSNP 420

Query: 421 TEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDERHHLIHEER 480
           TEELPE P +TER HESL  E+  IN IEKKKK E GSS DDSSDHDTD+E+HH+I  ER
Sbjct: 421 TEELPEVPLMTERNHESLKCEEVRINGIEKKKKPEFGSSCDDSSDHDTDEEQHHIITGER 480

Query: 481 IVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEEERMLTDVEG 540
           I++STDVEDITDGELWYELEKELQRQEKKVD   TREA VA   KEIKEEEE MLTDVEG
Sbjct: 481 IIASTDVEDITDGELWYELEKELQRQEKKVD-ANTREAKVATVGKEIKEEEESMLTDVEG 540

Query: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVGIYETPRELY 600
           SSEKPLSSLDASENIRFYPPGKTMHIVS PSP+SDNLVQDD+DE  QE VGIYETPRELY
Sbjct: 541 SSEKPLSSLDASENIRFYPPGKTMHIVSTPSPNSDNLVQDDEDESTQEIVGIYETPRELY 600

Query: 601 SKLRLSRTMINDHYMPMYKKMMELLINELERDVSSSYEM 640
           SKLRLSRTMINDHYMPMYKKMME LIN+LE+DV S+YEM
Sbjct: 601 SKLRLSRTMINDHYMPMYKKMMESLINQLEKDVISNYEM 638

BLAST of Lcy02g007220 vs. NCBI nr
Match: KAG6575088.1 (Sn1-specific diacylglycerol lipase alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 588/649 (90.60%), Postives = 615/649 (94.76%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLAAKGDEDDRS NLS+SIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFT+IRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRE+K+FSS+TCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRERKDFSSTTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQ AVKSAVVRTRSSL+SWSCMGARRRNGGLL NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 TEELPEAPRITERKHESLIAED--STINTIEKKKKLESGSSS---DDSSDHDTDDERHHL 480
           TEELPEAP  TERKHESLIA+D  +T NTI KKKKLESGSSS   DDSSDHDTDDERHHL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTILKKKKLESGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVSSTDVEDITDGELWYELEKELQRQEKKVDV---VITREADVAAAAKEIKEEE 540
           I+E++ + SSTDV+DITDGELWYELEKELQRQEKK      VITREA+VAA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTKTTTDVITREANVAAVAKEIVEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVG 600
           E MLT+VEGSSEKPLSSLDASENIRFYPPGKTMHIVS+PS DSDN     D+EIVQERVG
Sbjct: 541 ESMLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFV-HDNEIVQERVG 600

Query: 601 IYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSS-YEM 640
           IYETPR LYSKLRLSRTMINDHYMPMYKKM+ELLIN+LERDVSSS YEM
Sbjct: 601 IYETPRGLYSKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of Lcy02g007220 vs. NCBI nr
Match: KAG7013660.1 (Sn1-specific diacylglycerol lipase alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 587/649 (90.45%), Postives = 614/649 (94.61%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAGA+ATYAGAAL++YYLLSRRLAAKGDEDDRS NLS+SIRSGRRRISRRPAQAPATWF
Sbjct: 1   MAAGALATYAGAALLMYYLLSRRLAAKGDEDDRSDNLSESIRSGRRRISRRPAQAPATWF 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETITTLSETLRFTY+ETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGN+SVQL+GPEII
Sbjct: 61  ETITTLSETLRFTYAETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNDSVQLRGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
           AEL+SFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFT+IRDSNSKCF
Sbjct: 121 AELKSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTIIRDSNSKCF 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTP 
Sbjct: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPR 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRE+K+FSS+TCITFAPAACMTWELAESG
Sbjct: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILRERKDFSSTTCITFAPAACMTWELAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           KQFITTIIN SDLVPSFSAASIDDLR EVTASSWLNDLRDQVERTRVL+VVYRSASALGS
Sbjct: 301 KQFITTIINSSDLVPSFSAASIDDLRSEVTASSWLNDLRDQVERTRVLSVVYRSASALGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVKSAVVRTRSSLSSWSCMGARRRNGGLLSNP 420
           RLPSIATAKAKVAGAGALLRPVSTTTQ AVKSAVVRTRSSL+SWSCMGARRRNGGLL NP
Sbjct: 361 RLPSIATAKAKVAGAGALLRPVSTTTQTAVKSAVVRTRSSLASWSCMGARRRNGGLLPNP 420

Query: 421 TEELPEAPRITERKHESLIAED--STINTIEKKKKLESGSSS---DDSSDHDTDDERHHL 480
           TEELPEAP  TERKHESLIA+D  +T NTI KKKKLESGSSS   DDSSDHDTDDERHHL
Sbjct: 421 TEELPEAPLRTERKHESLIADDEVTTNNTILKKKKLESGSSSLGDDDSSDHDTDDERHHL 480

Query: 481 IHEER-IVSSTDVEDITDGELWYELEKELQRQEKKVDV---VITREADVAAAAKEIKEEE 540
           I+E++ + SSTDV+DITDGELWYELEKELQRQEKK      VITREA+VAA AKEI EEE
Sbjct: 481 INEDKTVASSTDVDDITDGELWYELEKELQRQEKKTKTTTDVITREANVAAVAKEIVEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDDDDEIVQERVG 600
           E MLT+VEGSSEKPLSSLDASENIRFYPPGKTMHIVS+PS DSDN     D+EIVQERVG
Sbjct: 541 ESMLTEVEGSSEKPLSSLDASENIRFYPPGKTMHIVSVPSSDSDNSFV-HDNEIVQERVG 600

Query: 601 IYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSSS-YEM 640
           IYETPR LY KLRLSRTMINDHYMPMYKKM+ELLIN+LERDVSSS YEM
Sbjct: 601 IYETPRGLYGKLRLSRTMINDHYMPMYKKMIELLINQLERDVSSSNYEM 648

BLAST of Lcy02g007220 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 779.6 bits (2012), Expect = 1.9e-225
Identity = 436/661 (65.96%), Postives = 506/661 (76.55%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAG M T  GA +I+Y L  R + A+  EDD  G L KS RSGRRRI RRPAQAPATW 
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDDSGGELGKSGRSGRRRIVRRPAQAPATWL 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETI+TLSETLRFTYSETLGKWPI DLAFGINYLMRRQGN   A+VYAG+  ++LKGPEII
Sbjct: 61  ETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
            +L   LR LT CMLFSKKPF +FLESAGY+ EDVL+QKPKAGI++PAFT+IRD+NSKC 
Sbjct: 121 MDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCI 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTA TGAVVPFHHSVLHDGG+SNLVLGYAH GMVAAARWIAKLS P 
Sbjct: 181 LLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPC 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LD+ P +K++IVGHSLGGGTA+LLTYILREQKEF+S+TC TFAPAACMTW+LAESG
Sbjct: 241 LLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAPAACMTWDLAESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           K FITTIINGSDLVP+FSA+S+DDLR EVT+SSW NDLRDQVE TRVL+VVYRSA+A+GS
Sbjct: 301 KHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATAIGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVK------SAVVRTRSSLSSWSCMGARRRN- 420
           RLPSIA+AKAKVAGAGA+LRPVS+ TQ  +K       AVV+TRS+LSSWSC+G RRR  
Sbjct: 361 RLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAI 420

Query: 421 GGLLSNPTEELPEAPRI--TERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDE 480
              L++   ++PEA  I    R  E+L+AE   I+  +  K+ E  SSS   SD D  DE
Sbjct: 421 SSQLNSKVTDMPEASAIMAERRSTEALLAETVAIDR-KGHKRTEHSSSSSSESDRDEPDE 480

Query: 481 RHH---LIHEERIVSSTD--VEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKE 540
                 LI  +++++ T    ED+T+GELW EL++EL RQE + D     E   AAAAKE
Sbjct: 481 EEEEEPLISIDQVIAETSSIEEDVTEGELWDELDRELTRQENERDSEAMEEE--AAAAKE 540

Query: 541 IKEEEERML---TDVEGSSEKPL--SSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDD 600
           I EEE  +        G ++ P+  SS+D  EN RFYPPGK MHIVS+   +S+     +
Sbjct: 541 ITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVSVTETESET----E 600

Query: 601 DDEIV------QERVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSS 637
            DE+V       ERV IYETPRELY K+RLSRTMINDHYMPMYKKMMELLI ELE D  S
Sbjct: 601 RDEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMELLITELECDSHS 654

BLAST of Lcy02g007220 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 755.4 bits (1949), Expect = 3.8e-218
Identity = 429/661 (64.90%), Postives = 498/661 (75.34%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRLAAKGDEDDRSGNLSKSIRSGRRRISRRPAQAPATWF 60
           MAAG M T  GA +I+Y L  R + A+  EDD  G L KS RSGRRRI RRPAQAPATW 
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDDSGGELGKSGRSGRRRIVRRPAQAPATWL 60

Query: 61  ETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEII 120
           ETI+TLSETLRFTYSETLGKWPI DLAFGINYLMRRQGN   A+VYAG+  ++LKGPEII
Sbjct: 61  ETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGPEII 120

Query: 121 AELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCF 180
            +L   LR LT CMLFSKKPF +FLESAGY+ EDVL+QKPKAGI++PAFT+IRD+NSKC 
Sbjct: 121 MDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCI 180

Query: 181 LLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPF 240
           LLLIRGTHSIKDTLTA TGAVVPFHHSVLHDGG+SNLVLGYAH GMVAAARWIAKLS P 
Sbjct: 181 LLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPC 240

Query: 241 LLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAESG 300
           LLK LD+ P +K++IVGHSLGGGTA+LLTYILREQKEF+S+TC TFAP        AESG
Sbjct: 241 LLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAP--------AESG 300

Query: 301 KQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASALGS 360
           K FITTIINGSDLVP+FSA+S+DDLR EVT+SSW NDLRDQVE TRVL+VVYRSA+A+GS
Sbjct: 301 KHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATAIGS 360

Query: 361 RLPSIATAKAKVAGAGALLRPVSTTTQAAVK------SAVVRTRSSLSSWSCMGARRRN- 420
           RLPSIA+AKAKVAGAGA+LRPVS+ TQ  +K       AVV+TRS+LSSWSC+G RRR  
Sbjct: 361 RLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAI 420

Query: 421 GGLLSNPTEELPEAPRI--TERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDE 480
              L++   ++PEA  I    R  E+L+AE   I+  +  K+ E  SSS   SD D  DE
Sbjct: 421 SSQLNSKVTDMPEASAIMAERRSTEALLAETVAIDR-KGHKRTEHSSSSSSESDRDEPDE 480

Query: 481 RHH---LIHEERIVSSTD--VEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKE 540
                 LI  +++++ T    ED+T+GELW EL++EL RQE + D     E   AAAAKE
Sbjct: 481 EEEEEPLISIDQVIAETSSIEEDVTEGELWDELDRELTRQENERDSEAMEEE--AAAAKE 540

Query: 541 IKEEEERML---TDVEGSSEKPL--SSLDASENIRFYPPGKTMHIVSIPSPDSDNLVQDD 600
           I EEE  +        G ++ P+  SS+D  EN RFYPPGK MHIVS+   +S+     +
Sbjct: 541 ITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVSVTETESET----E 600

Query: 601 DDEIV------QERVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINELERDVSS 637
            DE+V       ERV IYETPRELY K+RLSRTMINDHYMPMYKKMMELLI ELE D  S
Sbjct: 601 RDEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMELLITELECDSHS 646

BLAST of Lcy02g007220 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-179
Identity = 362/649 (55.78%), Postives = 456/649 (70.26%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRL-AAKGDEDDRSGNLSKSIRSGR-RRISRRPAQAPAT 60
           MA   MAT AGAA ++YY L+R+L A   D DD +   S S  S R  R+S R  QAPAT
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPAT 60

Query: 61  WFETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPE 120
           W ETI+TLSETLRFTYSETLGKWPIGDLAFGIN+L++RQG L V  V+ G +SV+L+G E
Sbjct: 61  WLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSE 120

Query: 121 IIAELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSK 180
           +  EL+  L LLT C  FSKK FP FLE  G+++E+VLI +PKAGILKPAFTV+ D N+K
Sbjct: 121 VATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTK 180

Query: 181 CFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLST 240
            FLLLIRGTHSIKDTLTA TGA+VPFHH+V+++ G+SNLVLGYAH GMVAAAR IAKL+T
Sbjct: 181 YFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLAT 240

Query: 241 PFLLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAE 300
           P LLKGL+ +PDYKIKIVGHSLGGGTAALLTYI+REQK  S++TC+TFAPAACMTWELA+
Sbjct: 241 PCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELAD 300

Query: 301 SGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASAL 360
           SG  FI ++ING+DLVP+FSAA++DDLR EVTAS+WLNDLR+Q+E TR+L+ VYRSA+AL
Sbjct: 301 SGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATAL 360

Query: 361 GSRLPSIATAKAKVAGAGALLRPVSTTTQAAVK---SAVVRTRSSLSSWSCMGARRRNGG 420
           GSRLPS+ATAKAKVAGAGA+LRPVS+ TQ  ++   S + R   S+SSWSCMG RRR   
Sbjct: 361 GSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRR--- 420

Query: 421 LLSNPTEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDERHHL 480
             ++ T+ + E    T       I E S    +  ++      S  + S+++    R   
Sbjct: 421 --ASATQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITGKWKSEAECSNYEETSPRLGA 480

Query: 481 IH----EERIVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEE 540
                 E+     T  E +T+ ELW +LE +L     +       E DV   AKEIKEEE
Sbjct: 481 TDLDECEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQ----PEETDV---AKEIKEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSI-PSPDSDNLVQDDDD------- 600
           E ++ +  G +     + +  E+ RF P GK MHIV++ P     N  +D+D        
Sbjct: 541 EAVIAEA-GVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALERPE 600

Query: 601 --EIVQE-RVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINEL 630
             E V+E RVGI+ TPR LYSK+RLS+ MI+DH+MP+Y++ +E LI EL
Sbjct: 601 TVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQEL 636

BLAST of Lcy02g007220 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-179
Identity = 362/649 (55.78%), Postives = 456/649 (70.26%), Query Frame = 0

Query: 1   MAAGAMATYAGAALIIYYLLSRRL-AAKGDEDDRSGNLSKSIRSGR-RRISRRPAQAPAT 60
           MA   MAT AGAA ++YY L+R+L A   D DD +   S S  S R  R+S R  QAPAT
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPAT 60

Query: 61  WFETITTLSETLRFTYSETLGKWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPE 120
           W ETI+TLSETLRFTYSETLGKWPIGDLAFGIN+L++RQG L V  V+ G +SV+L+G E
Sbjct: 61  WLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSE 120

Query: 121 IIAELQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSK 180
           +  EL+  L LLT C  FSKK FP FLE  G+++E+VLI +PKAGILKPAFTV+ D N+K
Sbjct: 121 VATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTK 180

Query: 181 CFLLLIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLST 240
            FLLLIRGTHSIKDTLTA TGA+VPFHH+V+++ G+SNLVLGYAH GMVAAAR IAKL+T
Sbjct: 181 YFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLAT 240

Query: 241 PFLLKGLDDFPDYKIKIVGHSLGGGTAALLTYILREQKEFSSSTCITFAPAACMTWELAE 300
           P LLKGL+ +PDYKIKIVGHSLGGGTAALLTYI+REQK  S++TC+TFAPAACMTWELA+
Sbjct: 241 PCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELAD 300

Query: 301 SGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSASAL 360
           SG  FI ++ING+DLVP+FSAA++DDLR EVTAS+WLNDLR+Q+E TR+L+ VYRSA+AL
Sbjct: 301 SGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATAL 360

Query: 361 GSRLPSIATAKAKVAGAGALLRPVSTTTQAAVK---SAVVRTRSSLSSWSCMGARRRNGG 420
           GSRLPS+ATAKAKVAGAGA+LRPVS+ TQ  ++   S + R   S+SSWSCMG RRR   
Sbjct: 361 GSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRR--- 420

Query: 421 LLSNPTEELPEAPRITERKHESLIAEDSTINTIEKKKKLESGSSSDDSSDHDTDDERHHL 480
             ++ T+ + E    T       I E S    +  ++      S  + S+++    R   
Sbjct: 421 --ASATQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITGKWKSEAECSNYEETSPRLGA 480

Query: 481 IH----EERIVSSTDVEDITDGELWYELEKELQRQEKKVDVVITREADVAAAAKEIKEEE 540
                 E+     T  E +T+ ELW +LE +L     +       E DV   AKEIKEEE
Sbjct: 481 TDLDECEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQ----PEETDV---AKEIKEEE 540

Query: 541 ERMLTDVEGSSEKPLSSLDASENIRFYPPGKTMHIVSI-PSPDSDNLVQDDDD------- 600
           E ++ +  G +     + +  E+ RF P GK MHIV++ P     N  +D+D        
Sbjct: 541 EAVIAEA-GVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALERPE 600

Query: 601 --EIVQE-RVGIYETPRELYSKLRLSRTMINDHYMPMYKKMMELLINEL 630
             E V+E RVGI+ TPR LYSK+RLS+ MI+DH+MP+Y++ +E LI EL
Sbjct: 601 TVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQEL 636

BLAST of Lcy02g007220 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 9.3e-23
Identity = 81/304 (26.64%), Postives = 143/304 (47.04%), Query Frame = 0

Query: 65  TLSETLRFTYSETLG--KWPIGDLAFGINYLMRRQGNLQVANVYAGNESVQLKGPEIIAE 124
           +LSE +       LG   W  GDL  G+  +  RQ +L   + +   + V++     + +
Sbjct: 131 SLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASL---SPFEDVKGVEVVSESTVYD 190

Query: 125 LQSFLRLLTFCMLFSKKPFPIFLESAGYSQEDVLIQKPKAGILKPAFTVIRDSNSKCFLL 184
           L     L   C   S        ++    + ++L     + +++P + +  D   K  + 
Sbjct: 191 LIYNAELAKGCYRDSVSG---LAKNTMLRENNILKFVKDSSVMRPGYYIGVDHRRKLVVF 250

Query: 185 LIRGTHSIKDTLTAVTGAVVPFHHSVLHDGGISNLVLGYAHFGMVAAARWIAKLSTPFLL 244
            IRGTH+I D +T +   V      V  +G  +       HFG   AARW        + 
Sbjct: 251 GIRGTHTIYDLITDI---VSSSDEEVTFEGYST-------HFGTAEAARWFLNHELQTIR 310

Query: 245 KGLDDFPDYKIKIVGHSLGGGTAALLTYIL----REQKEFSSS--TCITFAPAACMTWEL 304
           + L  +  YK+++VGHSLGG  A+L+  +L    RE+  F +   + + +A   C++ EL
Sbjct: 311 RCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCVSKEL 370

Query: 305 AESGKQFITTIINGSDLVPSFSAASIDDLRYEVTASSWLNDLRDQVERTRVLNVVYRSAS 361
           AE+  +F+TTI+   D++P  SAAS+  LR E+  + W + + ++ E   VL++V  +  
Sbjct: 371 AENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVI-EKEEWKNVLDLVTNAKQ 417