Lcy02g005430 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g005430
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	Chr02: 7086684 .. 7088952 (-)
RNA-Seq Expression	Lcy02g005430
Synteny	Lcy02g005430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAGCAAGCCTTGCAAATGGAGGCATCAAAACAAGAACCAAAAGAAATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCGAAGATTGACAAAGACATTGCTCGTTCTCTCTTTTTCGTGCACATGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCATTTGCATTATACATTATCACAGGTCTGGGTATCAGTGTTTCCTATCATAGAAATCTAGCTCATCGAAGTTTTAGCCTTCCCAAATGGCTCGAATACTTGTTTGCTTACTGTGGAGCTCATGCACTTCAGGTTTGCTCTCTTTATAAAGTTAGTACATGATGATTAAAAGTTAATAGATATATATCCATTGTAAGTTGAAAATTTTCATTGATTTTTGAATTTCAGGGTGATCCAATTGATTGGGTGAGTACGCACAGGTGTCACCATCGGTTTGTTGATACTGAAAAAGACCCACATAGTCCTATTCAAGGATTCTGGTTTAGCCACATTACTTGGGTTTTTGATTCCTATGCTTTGACTAAAAAAGTTTGTCCAAAATATTTCAATGATTTTCAAAAGATAGATAGAAACATCTTCATGTTGTTTCTGAAGCAAGGGAGACCAGAAAATGTTGGGGACTTGGAGAAGCAAGCATTCTATAGATTTATTCACAGGACATATTTCCTTCATCCAATTCTTCTTGCAGTCATTCTCTATACTCTGGGCGGAGCTCCCTTTCTAATATGGGGAATGGTAAAATGAAATTACTTGTCTTAACAGTTTTGCTATGCTTAGGTTCATAAATTAAAATATCTCTAAATATATATATATATATATATATATATATATATATATATACTCTCTGTGCATCTTCAAGAACTAAATTAGAATAAAAAGTTGTATCAAGTATGTGACCCCAATTTTCAGAGGGAGATTACCTAACTTAGCAAATGTTTATTATCGATCTTTACAATTCTACATATATAGGTTTCGAGAATTTTTTCTCTTTTTCTTTTAGTTCAATAATATATGGGGTTGAGAGATTCTAATCTTTTAACCTTTTAATTTATGACACATATTACACCTCAGTTGAGAGCTTTTCATGTTGGCGACTTCGAGAATTTGTGCTCTACAGATCATCTAAAGAAAATTAGCATCAAATAAATTTTTTCTTTTTTAAAAAATCTATTCTATTATATATATCTTTTTGTAGCATCTGGATTATAATTTTCTCCTCTTAAAAAATTAATGATCTAGTTAGTCATTAAAAATGTGTACAAAAGAAATATGACATAACAAATTCTGTCCTGCAGGGTGTGAGGATCGTAGCGGTCTTACATATAACATTTATGGTGAATTCAGTATGTCATATATGGGGAAAACGACAATGGAAAACAAACGATTTGTCAAGAAACAATTGGTAATTTTCTTTTTCTTTCTTTCCCATTATGAAGGAAAAAAAAAAGATTTAAATGGTTAAGGGCCTTTATTTCAATAAAGTAACACTCTTTAGGCTGTGAAGTAACTGAATAATTTTAGTGTTAATTTTGAAAATTAAAAAATTTGCATACAACCTTCCATTTATAGTAGCTAGTTATTTTTATTATTTTTTTAGTACAACAAACGTGGGGTATAGAGATTTGAACCTCCAACCTAAAGAGAGAATAGATATTGTAAGATATATCTTTTAAGCATACATCCTCGTATAATAAATTACTACGTGTTGTATATTTAATCTTTTTAAAACGATAATTCAAATAAGGAACAAACATAACCATAAAAGGCTTTCATTTGACAAATGCCAACTTGATTATATATTTTTTTAGTACAAAAAATATGTTTATTTTTGCATTGTATTTTACTATTTGTTATACTTTTCTTCCCCTTGTGTTTAGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATACTCAGCCAGACATGGACTTGAATGGTGGCAGATCGACTTTGGTTGGTACGTTGTTATGTTTCTTCGAGCCATTGGTGTAGCCACTAACATCAAACTACCCTCTCAAAGTCACAAGCAAAAGCTCGCACTCGAAAAATTACCTAAAGAAAGCTTTCAAAACTGATCCTTTACATATAAATTATCATACAAGTTTTATGCCAGTTAGATTATACTCTTGTTGGCACCTATATAAATTAGGTACTATTTGTAAAAATGTGTAATTCAAATATATAATGCAAAAGTTTATTTTCACGCTTCAATAAATTTTTG

mRNA sequence

TAAAGCAAGCCTTGCAAATGGAGGCATCAAAACAAGAACCAAAAGAAATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCGAAGATTGACAAAGACATTGCTCGTTCTCTCTTTTTCGTGCACATGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCATTTGCATTATACATTATCACAGGTCTGGGTATCAGTGTTTCCTATCATAGAAATCTAGCTCATCGAAGTTTTAGCCTTCCCAAATGGCTCGAATACTTGTTTGCTTACTGTGGAGCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGTACGCACAGGTGTCACCATCGGTTTGTTGATACTGAAAAAGACCCACATAGTCCTATTCAAGGATTCTGGTTTAGCCACATTACTTGGGTTTTTGATTCCTATGCTTTGACTAAAAAAGTTTGTCCAAAATATTTCAATGATTTTCAAAAGATAGATAGAAACATCTTCATGTTGTTTCTGAAGCAAGGGAGACCAGAAAATGTTGGGGACTTGGAGAAGCAAGCATTCTATAGATTTATTCACAGGACATATTTCCTTCATCCAATTCTTCTTGCAGTCATTCTCTATACTCTGGGCGGAGCTCCCTTTCTAATATGGGGAATGGGTGTGAGGATCGTAGCGGTCTTACATATAACATTTATGGTGAATTCAGTATGTCATATATGGGGAAAACGACAATGGAAAACAAACGATTTGTCAAGAAACAATTGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATACTCAGCCAGACATGGACTTGAATGGTGGCAGATCGACTTTGGTTGGTACGTTGTTATGTTTCTTCGAGCCATTGGTGTAGCCACTAACATCAAACTACCCTCTCAAAGTCACAAGCAAAAGCTCGCACTCGAAAAATTACCTAAAGAAAGCTTTCAAAACTGATCCTTTACATATAAATTATCATACAAGTTTTATGCCAGTTAGATTATACTCTTGTTGGCACCTATATAAATTAGGTACTATTTGTAAAAATGTGTAATTCAAATATATAATGCAAAAGTTTATTTTCACGCTTCAATAAATTTTTG

Coding sequence (CDS)

ATGGAGGCATCAAAACAAGAACCAAAAGAAATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCGAAGATTGACAAAGACATTGCTCGTTCTCTCTTTTTCGTGCACATGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCATTTGCATTATACATTATCACAGGTCTGGGTATCAGTGTTTCCTATCATAGAAATCTAGCTCATCGAAGTTTTAGCCTTCCCAAATGGCTCGAATACTTGTTTGCTTACTGTGGAGCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGTACGCACAGGTGTCACCATCGGTTTGTTGATACTGAAAAAGACCCACATAGTCCTATTCAAGGATTCTGGTTTAGCCACATTACTTGGGTTTTTGATTCCTATGCTTTGACTAAAAAAGTTTGTCCAAAATATTTCAATGATTTTCAAAAGATAGATAGAAACATCTTCATGTTGTTTCTGAAGCAAGGGAGACCAGAAAATGTTGGGGACTTGGAGAAGCAAGCATTCTATAGATTTATTCACAGGACATATTTCCTTCATCCAATTCTTCTTGCAGTCATTCTCTATACTCTGGGCGGAGCTCCCTTTCTAATATGGGGAATGGGTGTGAGGATCGTAGCGGTCTTACATATAACATTTATGGTGAATTCAGTATGTCATATATGGGGAAAACGACAATGGAAAACAAACGATTTGTCAAGAAACAATTGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATACTCAGCCAGACATGGACTTGAATGGTGGCAGATCGACTTTGGTTGGTACGTTGTTATGTTTCTTCGAGCCATTGGTGTAGCCACTAACATCAAACTACCCTCTCAAAGTCACAAGCAAAAGCTCGCACTCGAAAAATTACCTAAAGAAAGCTTTCAAAACTGA

Protein sequence

MEASKQEPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLGISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLALEKLPKESFQN

Homology

[Close]

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 6.9e-112
Identity = 177/295 (60.00%), Postives = 224/295 (75.93%), Query Frame = 0


Query: 20  KRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITG-LGISVSYHRNLAHR 79
           +R+W  +D      +  +H+L + APF FNW A  VAF LYI+TG LGI++S+HRNL+H+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 80  SFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWVF 139
           +F LPKWLEYLFAYCGA ALQG+PIDWVSTHR HH+F D+++DPHSP+ GFWFSH+ W+F
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 140 DSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPILL 199
           D+  +T++                       G P NVGDLEKQ FYRF+  TY LHP+ L
Sbjct: 215 DTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLAL 274

Query: 200 AVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILSF 259
           AV LY +GG PF++WGMGVRIV V HIT++VNS CH+WGK+ W T DLS+NNWWV  L+F
Sbjct: 275 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 334

Query: 260 GEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLA 314
           GEGWHNNHHAFE+SARHGLEWWQ+D  WYVV FL+AIG+AT++KLPS++ KQ++A
Sbjct: 335 GEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

[Close]

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-100
Identity = 162/305 (53.11%), Postives = 210/305 (68.85%), Query Frame = 0


Query: 10  EMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLGISV 69
           EM +  R+  +RKW ++D   A +  FVH LC+ APF+F W A  VA  +Y + GLGI+V
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITV 80

Query: 70  SYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGF 129
           SYHRNLAHRSF +PKWLEY FAYCG  A+QGDPIDWVSTHR HH+F D+++DPHSP +GF
Sbjct: 81  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 140

Query: 130 WFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHR 189
           WFSH+ W+FD+  L +K                       GR  NV DL++Q +Y+F+ R
Sbjct: 141 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 200

Query: 190 TYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRN 249
           T   H +    +LY  GG  FL WGMG+ +    H+T ++NS+CH+WG R WKTND SRN
Sbjct: 201 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 260

Query: 250 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHK 309
            WW+ + SFGE WHNNHHAFE SAR GLEWWQID  WY+V FL  IG+AT++KLPS+S +
Sbjct: 261 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 303

Query: 310 QKLAL 315
           +++A+
Sbjct: 321 RRMAM 303

[Close]

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 360.9 bits (925), Expect = 1.5e-98
Identity = 159/297 (53.54%), Postives = 202/297 (68.01%), Query Frame = 0


Query: 18  FGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLGISVSYHRNLAH 77
           F  R+W ++D     + F VH L + APF+F WSA WV F  Y I GLGI+VSYHRNLAH
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAH 90

Query: 78  RSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWV 137
           RSF +PKWLEYL AYC   A+QGDPIDWVSTHR HH+F D+E+DPHSP +GFWFSH+ W+
Sbjct: 91  RSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWI 150

Query: 138 FDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPIL 197
           +DS  L  K                       GR  NV DL++Q FYRF+ +T   H + 
Sbjct: 151 YDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHILG 210

Query: 198 LAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILS 257
           L   L+ LGG  F+ WGMGV     +H+T ++NS+CHIWG R WKTND SRN WW+ + S
Sbjct: 211 LGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFS 270

Query: 258 FGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLAL 315
           FGE WHNNHHAFE SAR GLEWWQID  WY+V F   IG+AT++K+P+++ ++++A+
Sbjct: 271 FGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAI 305

[Close]

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 357.5 bits (916), Expect = 1.6e-97
Identity = 161/306 (52.61%), Postives = 210/306 (68.63%), Query Frame = 0


Query: 11  MVKPLRSFGKRKWAKIDKDIARSLFFV--HMLCIFAPFHFNWSAFWVAFALYIITGLGIS 70
           +V+  RSF +R W     D+++ + FV  H+L + APF+F+W AFWV   L  I G+ I+
Sbjct: 79  VVRTKRSFWERSWT--SWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICIT 138

Query: 71  VSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQG 130
           +SYHRNL+HRSF LPKWLEYLFAY G  A QGDPI+WVS HR HH+  +T++DPHSP QG
Sbjct: 139 LSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQG 198

Query: 131 FWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIH 190
           FWFSH+ W+FD+ ++ +                        G  ENV DL +Q FYRF+ 
Sbjct: 199 FWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQ 258

Query: 191 RTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSR 250
           RT  LH +  + + Y  GG P L+WG+G+ I   LH+TF+VNSVCHIWG R W T+D S+
Sbjct: 259 RTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSK 318

Query: 251 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSH 310
           NNWWV IL+ GEGWHNNHHAFE+SARHGLEWWQ+D  W ++ FL AIG+ATN+KLP+++ 
Sbjct: 319 NNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 360

Query: 311 KQKLAL 315
            +  AL
Sbjct: 379 MKGKAL 360

[Close]

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.3e-97
Identity = 161/296 (54.39%), Postives = 206/296 (69.59%), Query Frame = 0


Query: 21  RKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGL-GISVSYHRNLAHRS 80
           RKW   D      +  +H+LC+FAP  F+W +FWV FALY I G+ G ++S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 81  FSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWVFD 140
           F LPK+LEY FAY G HALQGDP+ WVSTHR HH++ DT  DPHSPI+GFWF HI W+FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 141 SYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPILLA 200
           S         KY             +  K GR EN GDL KQ++YRF+ RT+  H  L A
Sbjct: 158 S---------KY-------------IIEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 201 VILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILSFG 260
            +LY  GG PF++WGM VR +   H++++VNSVCH WG R W T DLS NNW++ +L+ G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 261 EGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLALE 316
           EGWHNNHHAFEYSARHG+EWWQID  WY++  L  +G+AT+IK+PS+ HK+K++ +
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSFK 311

[Close]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 260/322 (80.75%), Postives = 295/322 (91.61%), Query Frame = 0


Query: 1   MEASKQEPKEMVKPLRSFGKR-KWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFAL 60
           MEA KQEPKEM  P +SFGKR KW K+DKD+A SLFFVH+L +FAPF+FNW AFW+AFAL
Sbjct: 21  MEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFAL 80

Query: 61  YIITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDT 120
           Y+ TGL GISVSYHRNLAH+SF+LPKWLEYLFAYCGAHALQGDPIDWV+THRCHHRFVDT
Sbjct: 81  YVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDT 140

Query: 121 EKDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDL 180
           E+DPHSPIQGFWFSHITW+ DSY+LTK+VCPKYF DFQK++RNIF++F K GRP+NVGDL
Sbjct: 141 ERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDL 200

Query: 181 EKQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGK 240
           EKQ FYRFIH+TYFLHPI+LA+ILY  GGAPFLIWGMGVRIV VLHIT M+NSVCHIWGK
Sbjct: 201 EKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGK 260

Query: 241 RQWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVA 300
           + W+T DLSRNNWWV ++SFGEGWHNNHHAFEYSARHG+EWWQIDFGWYVVMFL AIG+A
Sbjct: 261 QPWRTGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIA 320

Query: 301 TNIKLPSQSHKQKLALEKLPKE 321
           TN+K+PSQ+ KQKLA++  PKE
Sbjct: 321 TNVKVPSQNQKQKLAID-APKE 341

[Close]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 241/311 (77.49%), Postives = 280/311 (90.03%), Query Frame = 0


Query: 1   MEASKQEPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALY 60
           MEASKQEPKEM+KP R + +RKW K+DKDIA SLF VH+LCI APF FNWSAFWVAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  IITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTE 120
           I+TGL G+++SYHRNLAH+SF LPKWLEYLFAYCG  ALQGDP+DWVSTHRCHH+FVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 KDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLE 180
           +DPHSPI+GFWFSHI W+FDSY LTKKVCPKYF++FQK++RNIF+LF K G+PENV DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 181 KQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKR 240
           KQAFYRFIH+TY LHPI+LAVILY +GG PFL+WGM VRIV VLH T MVNSVCHIWGK+
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 241 QWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVAT 300
            W T D+S+NNWWV +++FGEGWHNNHHAFEYSA+ GLEWWQ+DFGWY+++FL+AIGVAT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

Query: 301 NIKLPSQSHKQ 311
           ++KLPSQSH Q
Sbjct: 301 HVKLPSQSHMQ 311

[Close]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL
Match: A0A6J1GWI3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457789 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.1e-144
Identity = 230/306 (75.16%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 7   EPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLG 66
           EPK MVKP  SF + KW K+D+ I+ +LF +H+LCIFAPFHFNW AFW++FALY+ITGL 
Sbjct: 3   EPKAMVKPPTSFMREKWTKLDQYISIALFILHILCIFAPFHFNWRAFWLSFALYVITGLS 62

Query: 67  ISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPI 126
           ISVSYHRNLAH+SF LPK LEY FAYC AHALQGDPIDWVSTHRCHH+FVDT++DPHSPI
Sbjct: 63  ISVSYHRNLAHKSFELPKVLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTDRDPHSPI 122

Query: 127 QGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRF 186
           QGFWFSHI W+FDSY LTK+VCPKYF DF+KI+R+I + F K GRP+NV DLEKQAFYRF
Sbjct: 123 QGFWFSHILWLFDSYTLTKRVCPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRF 182

Query: 187 IHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDL 246
           IH+TY LH +  AV+LY +GG PFLIWG  VRIV +LHITFMVNSVCHIWGKR+W T DL
Sbjct: 183 IHKTYLLHHVAFAVLLYEVGGLPFLIWGTCVRIVVLLHITFMVNSVCHIWGKRRWNTKDL 242

Query: 247 SRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQ 306
           S+NNW VG+L+FGEGWHNNHHAFEYSAR G+EWWQ D GWYV+ FL+AIGVAT++KLPSQ
Sbjct: 243 SKNNWLVGLLAFGEGWHNNHHAFEYSARLGIEWWQFDPGWYVIWFLQAIGVATHVKLPSQ 302

Query: 307 SHKQKL 313
            HKQ+L
Sbjct: 303 IHKQRL 308

[Close]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL
Match: A0A6J1IP09 (delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.6e-143
Identity = 229/316 (72.47%), Postives = 268/316 (84.81%), Query Frame = 0


Query: 2   EASKQEPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYI 61
           +ASKQEPKEMVKP RSF +RKW K+DK +A  +  +H+LCIFAPFHF W AF VA  L I
Sbjct: 19  DASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCIFAPFHFTWPAFRVAVTLQI 78

Query: 62  ITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEK 121
           ITGL G+++SYHRNLAH+SF +PKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTE+
Sbjct: 79  ITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTER 138

Query: 122 DPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEK 181
           DPHSPIQGFW SHI W+FDSY LT KV PKY ++ QKI++N+F+ F K  +PENVGDLE+
Sbjct: 139 DPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSNLQKIEKNMFVYFSKYRKPENVGDLER 198

Query: 182 QAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQ 241
           Q FYRFIH+TY LHPI LA ILYT+GG PFLIWGM VRIV + H TFMVNSVCH+WG++ 
Sbjct: 199 QTFYRFIHKTYLLHPIALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQH 258

Query: 242 WKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATN 301
           W+T DLSRNNWWVG+ SFGEGWHNNHHAF+YSAR GLEWWQ+D GWYV+ FL+ IG+AT+
Sbjct: 259 WRTGDLSRNNWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRFLQVIGLATD 318

Query: 302 IKLPSQSHKQKLALEK 317
           +KLPS +H Q LAL K
Sbjct: 319 VKLPSPAHMQALALNK 334

[Close]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL
Match: A0A1S4E0J1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494694 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.6e-143
Identity = 232/321 (72.27%), Postives = 271/321 (84.42%), Query Frame = 0


Query: 2   EASKQEPKEMVK-PLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALY 61
           E+ ++EPKEMVK P+RSF +RKW  +DK +A  + FVH+LCIFAPF FNWSAF VA AL 
Sbjct: 24  ESKQEEPKEMVKPPVRSFWRRKWTTLDKRVAYIISFVHLLCIFAPFQFNWSAFRVAVALK 83

Query: 62  IITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTE 121
           +ITGL G+++SYHRNLAH+SF LPKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTE
Sbjct: 84  VITGLFGMTISYHRNLAHKSFQLPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTE 143

Query: 122 KDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLE 181
           KDPHSPI GFW SH+ W+FDSY LT KVCPKY +D +KI+RN+F++F K G+P+NVGDLE
Sbjct: 144 KDPHSPINGFWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVGDLE 203

Query: 182 KQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKR 241
           KQ FYRFIH+TY LH + LA+ILY +GG PF IWGM VRIV V H TFMVNSVCHIWGK+
Sbjct: 204 KQRFYRFIHKTYMLHHLTLAIILYNVGGLPFFIWGMCVRIVVVFHSTFMVNSVCHIWGKQ 263

Query: 242 QWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVAT 301
            W+T DLSRNNW VG+ SFGEGWHNNHHAF YSAR GLEWWQ+D GWYV+ FL+ IG+AT
Sbjct: 264 PWRTGDLSRNNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIGLAT 323

Query: 302 NIKLPSQSHKQKLALEKLPKE 321
           N+KLPS SH Q LA++  PKE
Sbjct: 324 NVKLPSPSHMQNLAVDHKPKE 344

[Close]

BLAST of Lcy02g005430 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 582.0 bits (1499), Expect = 3.1e-162
Identity = 260/322 (80.75%), Postives = 295/322 (91.61%), Query Frame = 0


Query: 1   MEASKQEPKEMVKPLRSFGKR-KWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFAL 60
           MEA KQEPKEM  P +SFGKR KW K+DKD+A SLFFVH+L +FAPF+FNW AFW+AFAL
Sbjct: 21  MEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFAL 80

Query: 61  YIITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDT 120
           Y+ TGL GISVSYHRNLAH+SF+LPKWLEYLFAYCGAHALQGDPIDWV+THRCHHRFVDT
Sbjct: 81  YVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDT 140

Query: 121 EKDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDL 180
           E+DPHSPIQGFWFSHITW+ DSY+LTK+VCPKYF DFQK++RNIF++F K GRP+NVGDL
Sbjct: 141 ERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDL 200

Query: 181 EKQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGK 240
           EKQ FYRFIH+TYFLHPI+LA+ILY  GGAPFLIWGMGVRIV VLHIT M+NSVCHIWGK
Sbjct: 201 EKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGK 260

Query: 241 RQWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVA 300
           + W+T DLSRNNWWV ++SFGEGWHNNHHAFEYSARHG+EWWQIDFGWYVVMFL AIG+A
Sbjct: 261 QPWRTGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIA 320

Query: 301 TNIKLPSQSHKQKLALEKLPKE 321
           TN+K+PSQ+ KQKLA++  PKE
Sbjct: 321 TNVKVPSQNQKQKLAID-APKE 341

[Close]

BLAST of Lcy02g005430 vs. NCBI nr
Match: XP_011652978.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8649060.1 hypothetical protein Csa_015380 [Cucumis sativus])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 252/319 (79.00%), Postives = 287/319 (89.97%), Query Frame = 0


Query: 1   MEASKQEPKEM-VKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFAL 60
           ME SK E K+M VKPLRSF K+KWAKI++ +A SL FVH+LCIFAPFHFNWSAFWVAF L
Sbjct: 1   MEGSKLEQKDMAVKPLRSFRKKKWAKINRSVAGSLLFVHLLCIFAPFHFNWSAFWVAFVL 60

Query: 61  YIITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDT 120
           Y+ITGL GISVSYHRNLAHRSF+LPKWLEYLFAYCG HALQGDPI WV  HRCHHRFVDT
Sbjct: 61  YVITGLFGISVSYHRNLAHRSFNLPKWLEYLFAYCGVHALQGDPIGWVKIHRCHHRFVDT 120

Query: 121 EKDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPE-NVGD 180
           EKDPHSPIQGF FSH+TW+ DSY LT+KV PKYF+D++KI+RN+ M+ +KQGRPE NVGD
Sbjct: 121 EKDPHSPIQGFLFSHVTWLLDSYVLTEKVGPKYFDDYEKIERNVVMVNMKQGRPENNVGD 180

Query: 181 LEKQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWG 240
           LEKQAFYRF+ +TYFLH +LLAV+LY +GG PFLIWGMGVRIV VLHITFMVNSVCHIWG
Sbjct: 181 LEKQAFYRFLRKTYFLHLLLLAVLLYAMGGVPFLIWGMGVRIVVVLHITFMVNSVCHIWG 240

Query: 241 KRQWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGV 300
           K+ WKTNDLS NNW VG+L FGEGWHNNHHAFEYSARHGLEWW+IDFGWYV+MFL+AIGV
Sbjct: 241 KQLWKTNDLSTNNWLVGLLGFGEGWHNNHHAFEYSARHGLEWWEIDFGWYVIMFLQAIGV 300

Query: 301 ATNIKLPSQSHKQKLALEK 317
           AT++K+P Q HK+KLA+++
Sbjct: 301 ATHVKVPLQHHKKKLAMDE 319

[Close]

BLAST of Lcy02g005430 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 241/311 (77.49%), Postives = 280/311 (90.03%), Query Frame = 0


Query: 1   MEASKQEPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALY 60
           MEASKQEPKEM+KP R + +RKW K+DKDIA SLF VH+LCI APF FNWSAFWVAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  IITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTE 120
           I+TGL G+++SYHRNLAH+SF LPKWLEYLFAYCG  ALQGDP+DWVSTHRCHH+FVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 KDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLE 180
           +DPHSPI+GFWFSHI W+FDSY LTKKVCPKYF++FQK++RNIF+LF K G+PENV DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 181 KQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKR 240
           KQAFYRFIH+TY LHPI+LAVILY +GG PFL+WGM VRIV VLH T MVNSVCHIWGK+
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 241 QWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVAT 300
            W T D+S+NNWWV +++FGEGWHNNHHAFEYSA+ GLEWWQ+DFGWY+++FL+AIGVAT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

Query: 301 NIKLPSQSHKQ 311
           ++KLPSQSH Q
Sbjct: 301 HVKLPSQSHMQ 311

[Close]

BLAST of Lcy02g005430 vs. NCBI nr
Match: XP_038903746.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 1.7e-144
Identity = 229/322 (71.12%), Postives = 270/322 (83.85%), Query Frame = 0


Query: 1   MEASKQEPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALY 60
           M+ SKQEP E+VKPL  F + KW  IDK  A  +FF+H+ CIF PF F W AFWVAF LY
Sbjct: 1   MDVSKQEPSELVKPLTPFERGKWNNIDKTTAFVIFFIHIPCIFGPFQFTWGAFWVAFVLY 60

Query: 61  IITGL-GISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTE 120
           +ITGL GI++SYHR+L+H+SF LPKWLEYLFAYCG HALQGDP+DWVSTHRCHH+FVDTE
Sbjct: 61  VITGLFGITISYHRHLSHKSFKLPKWLEYLFAYCGVHALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 KDPHSPIQGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLE 180
           KDPHSPI+GFWFSHI W  DSY LTKKVCPKYF DF+ ++RN+ ML LK GRP NV DLE
Sbjct: 121 KDPHSPIEGFWFSHIAWTLDSYLLTKKVCPKYFRDFKGMERNMLMLNLKHGRPTNVVDLE 180

Query: 181 KQAFYRFIHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKR 240
           KQ+FYRFI +TYFLHPI LAV+LY +GG PF+IWGM VR+   +H+ FMVNS+ H WG R
Sbjct: 181 KQSFYRFIQKTYFLHPIALAVVLYAIGGIPFVIWGMFVRVTMFVHVMFMVNSIGHRWGTR 240

Query: 241 QWKTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVAT 300
           QW T+DLS NNWW+ +LSFG+GWHNNHHAFEYSARHGLEWWQIDF WYV+MFL+AIGVAT
Sbjct: 241 QWNTSDLSTNNWWLSLLSFGDGWHNNHHAFEYSARHGLEWWQIDFSWYVIMFLQAIGVAT 300

Query: 301 NIKLPSQSHKQKLALEKLPKES 322
            +KLP+QSHK++LA+ KL  ++
Sbjct: 301 EVKLPTQSHKERLAMHKLKDDT 322

[Close]

BLAST of Lcy02g005430 vs. NCBI nr
Match: XP_023526679.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 5.0e-144
Identity = 230/306 (75.16%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 7   EPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLG 66
           EPK MVKP  SF + KW K+D+ I+ +LF +H+LCIFAPFHFNW AFWV+FALY+ITGL 
Sbjct: 3   EPKAMVKPPTSFMREKWTKLDQYISIALFILHILCIFAPFHFNWRAFWVSFALYVITGLS 62

Query: 67  ISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPI 126
           ISVSYHRNLAH+SF LPK LEY FAYC AHALQGDPIDWVSTHRCHH+FVDTE+DPHSP+
Sbjct: 63  ISVSYHRNLAHKSFELPKLLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPV 122

Query: 127 QGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRF 186
           QGFWFSHI W+FDSY LTK+VCPKYF +F+KI+R+I + F K GRP+NV DLEKQAFYRF
Sbjct: 123 QGFWFSHILWLFDSYTLTKRVCPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRF 182

Query: 187 IHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDL 246
           IH+TY LH +  AV+LY +GG PFLIWGM VRIV +LHITFMVNSVCHIWGKR+W T DL
Sbjct: 183 IHKTYLLHHVAFAVLLYEVGGLPFLIWGMCVRIVVLLHITFMVNSVCHIWGKRRWNTKDL 242

Query: 247 SRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQ 306
           S+NNW VG+L+FGEGWHNNHHAFEYSAR G+EWW+ D GWYV+ FL+AIGVAT +KLPSQ
Sbjct: 243 SKNNWLVGLLAFGEGWHNNHHAFEYSARFGIEWWEYDPGWYVIRFLQAIGVATQVKLPSQ 302

Query: 307 SHKQKL 313
            HKQ+L
Sbjct: 303 IHKQRL 308

[Close]

BLAST of Lcy02g005430 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 405.2 bits (1040), Expect = 4.9e-113
Identity = 177/295 (60.00%), Postives = 224/295 (75.93%), Query Frame = 0


Query: 20  KRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITG-LGISVSYHRNLAHR 79
           +R+W  +D      +  +H+L + APF FNW A  VAF LYI+TG LGI++S+HRNL+H+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 80  SFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWVF 139
           +F LPKWLEYLFAYCGA ALQG+PIDWVSTHR HH+F D+++DPHSP+ GFWFSH+ W+F
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 140 DSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPILL 199
           D+  +T++                       G P NVGDLEKQ FYRF+  TY LHP+ L
Sbjct: 215 DTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLAL 274

Query: 200 AVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILSF 259
           AV LY +GG PF++WGMGVRIV V HIT++VNS CH+WGK+ W T DLS+NNWWV  L+F
Sbjct: 275 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 334

Query: 260 GEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLA 314
           GEGWHNNHHAFE+SARHGLEWWQ+D  WYVV FL+AIG+AT++KLPS++ KQ++A
Sbjct: 335 GEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

[Close]

BLAST of Lcy02g005430 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 367.9 bits (943), Expect = 8.7e-102
Identity = 162/305 (53.11%), Postives = 210/305 (68.85%), Query Frame = 0


Query: 10  EMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLGISV 69
           EM +  R+  +RKW ++D   A +  FVH LC+ APF+F W A  VA  +Y + GLGI+V
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITV 80

Query: 70  SYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGF 129
           SYHRNLAHRSF +PKWLEY FAYCG  A+QGDPIDWVSTHR HH+F D+++DPHSP +GF
Sbjct: 81  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 140

Query: 130 WFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHR 189
           WFSH+ W+FD+  L +K                       GR  NV DL++Q +Y+F+ R
Sbjct: 141 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 200

Query: 190 TYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRN 249
           T   H +    +LY  GG  FL WGMG+ +    H+T ++NS+CH+WG R WKTND SRN
Sbjct: 201 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 260

Query: 250 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHK 309
            WW+ + SFGE WHNNHHAFE SAR GLEWWQID  WY+V FL  IG+AT++KLPS+S +
Sbjct: 261 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 303

Query: 310 QKLAL 315
           +++A+
Sbjct: 321 RRMAM 303

[Close]

BLAST of Lcy02g005430 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 360.9 bits (925), Expect = 1.1e-99
Identity = 159/297 (53.54%), Postives = 202/297 (68.01%), Query Frame = 0


Query: 18  FGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLGISVSYHRNLAH 77
           F  R+W ++D     + F VH L + APF+F WSA WV F  Y I GLGI+VSYHRNLAH
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAH 90

Query: 78  RSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQGFWFSHITWV 137
           RSF +PKWLEYL AYC   A+QGDPIDWVSTHR HH+F D+E+DPHSP +GFWFSH+ W+
Sbjct: 91  RSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWI 150

Query: 138 FDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIHRTYFLHPIL 197
           +DS  L  K                       GR  NV DL++Q FYRF+ +T   H + 
Sbjct: 151 YDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHILG 210

Query: 198 LAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSRNNWWVGILS 257
           L   L+ LGG  F+ WGMGV     +H+T ++NS+CHIWG R WKTND SRN WW+ + S
Sbjct: 211 LGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFS 270

Query: 258 FGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSHKQKLAL 315
           FGE WHNNHHAFE SAR GLEWWQID  WY+V F   IG+AT++K+P+++ ++++A+
Sbjct: 271 FGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAI 305

[Close]

BLAST of Lcy02g005430 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-98
Identity = 161/306 (52.61%), Postives = 210/306 (68.63%), Query Frame = 0


Query: 11  MVKPLRSFGKRKWAKIDKDIARSLFFV--HMLCIFAPFHFNWSAFWVAFALYIITGLGIS 70
           +V+  RSF +R W     D+++ + FV  H+L + APF+F+W AFWV   L  I G+ I+
Sbjct: 79  VVRTKRSFWERSWT--SWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICIT 138

Query: 71  VSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPIQG 130
           +SYHRNL+HRSF LPKWLEYLFAY G  A QGDPI+WVS HR HH+  +T++DPHSP QG
Sbjct: 139 LSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQG 198

Query: 131 FWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRFIH 190
           FWFSH+ W+FD+ ++ +                        G  ENV DL +Q FYRF+ 
Sbjct: 199 FWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQ 258

Query: 191 RTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDLSR 250
           RT  LH +  + + Y  GG P L+WG+G+ I   LH+TF+VNSVCHIWG R W T+D S+
Sbjct: 259 RTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSK 318

Query: 251 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQSH 310
           NNWWV IL+ GEGWHNNHHAFE+SARHGLEWWQ+D  W ++ FL AIG+ATN+KLP+++ 
Sbjct: 319 NNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 360

Query: 311 KQKLAL 315
            +  AL
Sbjct: 379 MKGKAL 360

[Close]

BLAST of Lcy02g005430 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.1e-91
Identity = 151/310 (48.71%), Postives = 205/310 (66.13%), Query Frame = 0


Query: 7   EPKEMVKPLRSFGKRKWAKIDKDIARSLFFVHMLCIFAPFHFNWSAFWVAFALYIITGLG 66
           + K + K  R++  RKW + D   A ++  VH+LC+ APF++ W AF     L I+T L 
Sbjct: 12  QKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC 71

Query: 67  ISVSYHRNLAHRSFSLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTEKDPHSPI 126
           I+ SYHRNL HRSF LPKWLEY FAY    ALQGDP+DWVS HR HH+F D+++DPHSPI
Sbjct: 72  ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPI 131

Query: 127 QGFWFSHITWVFDSYALTKKVCPKYFNDFQKIDRNIFMLFLKQGRPENVGDLEKQAFYRF 186
           +GFWFSH+ W+FD+  + +K                       GR  NV DL++Q FYRF
Sbjct: 132 EGFWFSHVLWIFDTDYIREKC----------------------GRRNNVMDLKQQWFYRF 191

Query: 187 IHRTYFLHPILLAVILYTLGGAPFLIWGMGVRIVAVLHITFMVNSVCHIWGKRQWKTNDL 246
           + +T  LH +    ++Y  GG P+L W +G   V   H T++VNS CHI G + W+TND 
Sbjct: 192 LKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDT 251

Query: 247 SRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVMFLRAIGVATNIKLPSQ 306
           SRN WW+ +L+ GE WHNNHHAFE SARHGLEW+Q+D  WY++ F +A+G+ATN+KLP+ 
Sbjct: 252 SRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTD 299

Query: 307 SHKQKLALEK 317
           + K+K+A+ +
Sbjct: 312 AQKRKMAIRR 299

The following BLAST results are available for this feature:

BLAST of Lcy02g005430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	6.9e-112	60.00	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	1.2e-100	53.11	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
Q9SID2	1.5e-98	53.54	Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=...	[more]
Q9LVZ3	1.6e-97	52.61	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P0DOW2	6.3e-97	54.39	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]

BLAST of Lcy02g005430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHZ7	1.5e-162	80.75	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DJP0	1.3e-153	77.49	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GWI3	7.1e-144	75.16	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1IP09	4.6e-143	72.47	delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233...	[more]
A0A1S4E0J1	4.6e-143	72.27	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]

BLAST of Lcy02g005430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153632.1	3.1e-162	80.75	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_011652978.2	2.7e-153	79.00	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi...	[more]
XP_022153644.1	2.7e-153	77.49	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_038903746.1	1.7e-144	71.12	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_023526679.1	5.0e-144	75.16	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is...	[more]

BLAST of Lcy02g005430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	4.9e-113	60.00	fatty acid desaturase 5	[more]
AT1G06080.1	8.7e-102	53.11	delta 9 desaturase 1	[more]
AT2G31360.1	1.1e-99	53.54	16:0delta9 desaturase 2	[more]
AT3G15870.1	1.2e-98	52.61	Fatty acid desaturase family protein	[more]
AT1G06100.1	3.1e-91	48.71	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 109..138 score: 49.47 coord: 49..71 score: 25.86 coord: 72..92 score: 30.33
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 20..316
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 49..302 e-value: 1.01936E-72 score: 220.505
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 50..284 e-value: 9.6E-21 score: 74.8
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 20..316

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g005430.1	Lcy02g005430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water