Lcy02g005400 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g005400
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Delta 9 desaturase
Location	Chr02: 6994581 .. 6996909 (+)
RNA-Seq Expression	Lcy02g005400
Synteny	Lcy02g005400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTATTCTTGTGTTTCACCAATGGAGGCAACAAGGAAAGAGCTTGAAGATAAAAATGATAAGAAGGTGACATTTTCAGTGAAGTCCAAACGAGCTCTTCTAAAGAGAAAATGGACAACATTAGATCGACGTTCTGCTGGTGGGATTTTGTTCTTGCATCTGCTTTGTCTCTTGGCCCCATTTCACTACAATTGGTCAGCCTTTTGGATTGCCTTTGCACTATATTTTATTACCGGGGTACTCGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGTTTCAAACTTCCCAAATGGCTCGAGTACACATTCGCTTATTTGGGAGTGCAAGCCTTTCAGGTTTTTTTTTTTTTTTTCATTTCCTATTTAGTGTCGTGGGTTTGTTAATTTAAAGTTATACGATGTTGTAGTAATGTGAATATAATTTTTTATTTTATTTTATTATGCTACAGGGTGACCCAATTGATTGGGTTAGTACGCATAGATATCATCACCAGTTTGTTGATACAGAAAGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACCTGACTTGGATGTTCGACTCATATGCTTTGACCCACAAGATTTACCCGAAAAACCTCGAAGATTTCGCGGAAAGAGATTCAAAAAGAAAACTTTCTAGCAATATGTTTGTGGGATTTCTAAAGTATGGAAGGCCAAAGAATGTTGAGGATCTGCAGAAACAAGCCTTTTATAGGTTTCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTCCTCTATGCAATTGGAGGACTGTCCTTTGTTGTTTGGGGAATGGTAACTATTAAACGTAGAGATTTTTATTTTTATTTTATTATTTTATTTATTTTCAAAGTCGTGACAAGAAATCTCTCTTTTTATTTGCTTTTATTTTTAAAAATCTTAGTTTCTGCATGCTCTATTTATTGTGCACTGAGAACAAAACTTGGCTACAGAGCTCGAGGTAAGAAAACTTGTCTCCCCTATTAAATTTTCCTGATCTGTGTACTTTTACTATTTTATTTTTATTAAAATATATTCTTTTAAAACACTTCTTATGGTTGCATTTTCTAAAAATATATTAATAGTGGTTTTATAAATGTTAAAATCGTGTTTGATAACGATTTGATTTTTTTATAAAAAAAATTATATATACTCGTTTTCTCATCATTTTGTTGCAATTAATTTTTTTTTTTATTAAGAAAACATGTAAATATTCTTAACAAAATTTTAGGAAAAAAAAAAGCTTTTAAAAACTAATGGTAAAGATATTTTAAAAACAAGCTTTTAATTTCTTTACCATGAGTTTTCATCTTTTTCTTAAAGAAACATGTAAATTCTTAGCTAAATTGTAGAAACAAAAACAAGTTTTTGAAAACATTTGTAAGTAGATAACAAAACAAAGAAACCCATGGATGGAAGTAATGTTTTATTAGCTCTAACCTCTTAGTCCAGAGTTGATCAGAACCGGATACAAGCTTGCTTTATGCTGGTTGAACTATGTTCATATTGAAAAAAAAAAAAAAAACAACCTACCTCATATATATGTAGCCAATGTTTTTCTCTCCATATTTTTATGGATTTACTTATATGATCATTATATCTAAAAAAATTACATATCTCATAGTTAAAAAAATATTTATCATTATATCTAAAAAAATTCACAAGGAGATGGTAAGAAAATTAATGAGAGTTTGATGACGAATAATAATGGCCATGGCAGGGAGTGAGGCTTATTTGTGTGCTACACGCTACTTTCTTCGTAAATTCAGCATGTCACATTTGGGGCTACAGAAAATGGAACACGAATGATTTGTCGAAGAACAATTGGTGCTGTCACTTTTTCCTTTTCTTTTAGATTCATTGTCTTTTTTTTTTCCCATAATTAAGGTTGATGTGTTTTTAATTTGTGTTTATGTGATATATTTTGATTAGGGTGATAGGGTTGGTTGCTTGTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATACTCAGCTCGGCATGGCCTTCAATGGTGGGAATTTGACTTCACATGGTGGGTTATCTTGTTCCTTCAAGCTCTTGGATTGGCAACTGATGTGAAATTACCCTCTCAACTTCATATGCAAAAGTTGGCTCTCCAACGTCACAACGCTTGATGTTTTTTTACGACCCGTTTGGATTACGGATGTTTTATGACGAGTATGTAGATATTAAATATGTGGGAGTTGAAAATCCAGAATTTAAATGTCTAGAATGATGAATATCACTGTTTGGATATGCACGATAATTAATATTTGAGATATGGATGTTTTGTTT

mRNA sequence

GCTTATTCTTGTGTTTCACCAATGGAGGCAACAAGGAAAGAGCTTGAAGATAAAAATGATAAGAAGGTGACATTTTCAGTGAAGTCCAAACGAGCTCTTCTAAAGAGAAAATGGACAACATTAGATCGACGTTCTGCTGGTGGGATTTTGTTCTTGCATCTGCTTTGTCTCTTGGCCCCATTTCACTACAATTGGTCAGCCTTTTGGATTGCCTTTGCACTATATTTTATTACCGGGGTACTCGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGTTTCAAACTTCCCAAATGGCTCGAGTACACATTCGCTTATTTGGGAGTGCAAGCCTTTCAGGGTGACCCAATTGATTGGGTTAGTACGCATAGATATCATCACCAGTTTGTTGATACAGAAAGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACCTGACTTGGATGTTCGACTCATATGCTTTGACCCACAAGATTTACCCGAAAAACCTCGAAGATTTCGCGGAAAGAGATTCAAAAAGAAAACTTTCTAGCAATATGTTTGTGGGATTTCTAAAGTATGGAAGGCCAAAGAATGTTGAGGATCTGCAGAAACAAGCCTTTTATAGGTTTCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTCCTCTATGCAATTGGAGGACTGTCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTTATTTGTGTGCTACACGCTACTTTCTTCGTAAATTCAGCATGTCACATTTGGGGCTACAGAAAATGGAACACGAATGATTTGTCGAAGAACAATTGGGTGATAGGGTTGGTTGCTTGTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATACTCAGCTCGGCATGGCCTTCAATGGTGGGAATTTGACTTCACATGGTGGGTTATCTTGTTCCTTCAAGCTCTTGGATTGGCAACTGATGTGAAATTACCCTCTCAACTTCATATGCAAAAGTTGGCTCTCCAACGTCACAACGCTTGATGTTTTTTTACGACCCGTTTGGATTACGGATGTTTTATGACGAGTATGTAGATATTAAATATGTGGGAGTTGAAAATCCAGAATTTAAATGTCTAGAATGATGAATATCACTGTTTGGATATGCACGATAATTAATATTTGAGATATGGATGTTTTGTTT

Coding sequence (CDS)

ATGGAGGCAACAAGGAAAGAGCTTGAAGATAAAAATGATAAGAAGGTGACATTTTCAGTGAAGTCCAAACGAGCTCTTCTAAAGAGAAAATGGACAACATTAGATCGACGTTCTGCTGGTGGGATTTTGTTCTTGCATCTGCTTTGTCTCTTGGCCCCATTTCACTACAATTGGTCAGCCTTTTGGATTGCCTTTGCACTATATTTTATTACCGGGGTACTCGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGTTTCAAACTTCCCAAATGGCTCGAGTACACATTCGCTTATTTGGGAGTGCAAGCCTTTCAGGGTGACCCAATTGATTGGGTTAGTACGCATAGATATCATCACCAGTTTGTTGATACAGAAAGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACCTGACTTGGATGTTCGACTCATATGCTTTGACCCACAAGATTTACCCGAAAAACCTCGAAGATTTCGCGGAAAGAGATTCAAAAAGAAAACTTTCTAGCAATATGTTTGTGGGATTTCTAAAGTATGGAAGGCCAAAGAATGTTGAGGATCTGCAGAAACAAGCCTTTTATAGGTTTCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTCCTCTATGCAATTGGAGGACTGTCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTTATTTGTGTGCTACACGCTACTTTCTTCGTAAATTCAGCATGTCACATTTGGGGCTACAGAAAATGGAACACGAATGATTTGTCGAAGAACAATTGGGTGATAGGGTTGGTTGCTTGTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATACTCAGCTCGGCATGGCCTTCAATGGTGGGAATTTGACTTCACATGGTGGGTTATCTTGTTCCTTCAAGCTCTTGGATTGGCAACTGATGTGAAATTACCCTCTCAACTTCATATGCAAAAGTTGGCTCTCCAACGTCACAACGCTTGA

Protein sequence

MEATRKELEDKNDKKVTFSVKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLPSQLHMQKLALQRHNA

Homology

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BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-111
Identity = 181/308 (58.77%), Postives = 225/308 (73.05%), Query Frame = 0


Query: 21  KSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLSY 80
           K K    +R+W  +D  +   +L +HLL LLAPF +NW A  +AF LY +TG+LGITLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 81  HRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWF 140
           HRNL+H++FKLPKWLEY FAY G QA QG+PIDWVSTHRYHHQF D++RDPHSPL+GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 141 SHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFYR 200
           SH+ WMFD+  +T +                             G P NV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 201 FLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTND 260
           FLR TY+LHP+ALA+ LYA+GG  F+VWGMGVR++ V H T+ VNSACH+WG + WNT D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 327

Query: 261 LSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLPS 320
           LSKNNW +  +A GEGWHNNHHAFE+SARHGL+WW+ D TW+V+ FLQA+GLATDVKLPS
Sbjct: 328 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPS 367

Query: 321 QLHMQKLA 329
           +   Q++A
Sbjct: 388 EAQKQRMA 367

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BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-95
Identity = 168/334 (50.30%), Postives = 216/334 (64.67%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFSV---KSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYN 60
           M  +  E E+ N K         + KRA+ +RKW  LD   A   LF+H LCLLAPF++ 
Sbjct: 1   MSLSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFT 60

Query: 61  WSAFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVST 120
           W A  +A  +Y + G LGIT+SYHRNLAHRSFK+PKWLEY FAY G+ A QGDPIDWVST
Sbjct: 61  WPALRVALIVYTVGG-LGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVST 120

Query: 121 HRYHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNM 180
           HRYHHQF D++RDPHSP EGFWFSHL W+FD+  L  K                      
Sbjct: 121 HRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC--------------------- 180

Query: 181 FVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICV 240
                  GR  NVEDL++Q +Y+FL++T L H +    +LY  GGLSF+ WGMG+ +   
Sbjct: 181 -------GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAME 240

Query: 241 LHATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEF 300
            H T  +NS CH+WG R W TND S+N W + + + GE WHNNHHAFE SAR GL+WW+ 
Sbjct: 241 HHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQI 300

Query: 301 DFTWWVILFLQALGLATDVKLPSQLHMQKLALQR 332
           D +W+++ FL+ +GLATDVKLPS+   +++A+ R
Sbjct: 301 DISWYIVRFLEIIGLATDVKLPSESQRRRMAMVR 305

[Close]

BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 349.0 bits (894), Expect = 6.0e-95
Identity = 162/310 (52.26%), Postives = 211/310 (68.06%), Query Frame = 0


Query: 20  VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLS 79
           V++KR+  +R WT+ D       +  HLL LLAPF+++W AFW+   L FI G+  ITLS
Sbjct: 80  VRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLS 139

Query: 80  YHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFW 139
           YHRNL+HRSF LPKWLEY FAY GV AFQGDPI+WVS HRYHH+  +T+RDPHSP +GFW
Sbjct: 140 YHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFW 199

Query: 140 FSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFY 199
           FSH+ W+FD+ ++                                G  +NV+DL +Q FY
Sbjct: 200 FSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPFY 259

Query: 200 RFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTN 259
           RFL++T LLH +A + + Y  GG+  +VWG+G+ +   LH TF VNS CHIWG R WNT+
Sbjct: 260 RFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTS 319

Query: 260 DLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLP 319
           D SKNNW + ++  GEGWHNNHHAFE+SARHGL+WW+ D TW +I FL+A+GLAT+VKLP
Sbjct: 320 DFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLP 360

Query: 320 SQLHMQKLAL 330
           ++  M+  AL
Sbjct: 380 TETQMKGKAL 360

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BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.0e-94
Identity = 165/330 (50.00%), Postives = 212/330 (64.24%), Query Frame = 0


Query: 2   EATRKELEDKND-KKVTFSVKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSA 61
           E T    ED+    +V    K+K+    RKW + D      I  LH+LCL AP  ++W +
Sbjct: 10  ETTAAAEEDRLPCSEVPVKEKTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKS 69

Query: 62  FWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRY 121
           FW+ FALY I GV G TLS+HRNL HRSFKLPK+LEY FAY+G+ A QGDP+ WVSTHRY
Sbjct: 70  FWVCFALYAICGVFGTTLSFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRY 129

Query: 122 HHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVG 181
           HH++ DT  DPHSP+EGFWF H+ W+FDS  +  K                         
Sbjct: 130 HHKYTDTYLDPHSPIEGFWFCHIFWLFDSKYIIEKC------------------------ 189

Query: 182 FLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHA 241
               GR +N  DL KQ++YRFL +T++ H    A +LY  GG  F+VWGM VR I   H 
Sbjct: 190 ----GRYENAGDLMKQSYYRFLERTFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHL 249

Query: 242 TFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFT 301
           ++ VNS CH WG R WNT DLS NNW I ++  GEGWHNNHHAFEYSARHG++WW+ D T
Sbjct: 250 SWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTT 309

Query: 302 WWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           W++I  L+ LGLATD+K+PS++H +K++ +
Sbjct: 310 WYIIKLLEYLGLATDIKVPSEIHKRKMSFK 311

[Close]

BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.0e-94
Identity = 173/324 (53.40%), Postives = 216/324 (66.67%), Query Frame = 0


Query: 5   RKELEDKNDKKVTFSVKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIA 64
           + +LE     +V    K+K    +RKW   D ++A  +L LH L  +APF+++W AFWI+
Sbjct: 56  KDDLERFRTSEVVLERKAK-GFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWIS 115

Query: 65  FALY-FITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQ 124
           F L  F +GVLGITL +HR L H  FKLPK +EY FAY G  A QGDP++WVS HRYHHQ
Sbjct: 116 FILLGFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQ 175

Query: 125 FVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLK 184
           FVDTERD HSP +GFWF H+ W+ D          K+L  F E+               +
Sbjct: 176 FVDTERDVHSPTQGFWFCHIGWVLD----------KDL--FVEK---------------R 235

Query: 185 YGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFF 244
            GR  NV DL+KQAFYRFL+KTY+ H +AL  +LY +GG  ++VWGMG RL+ + H+TF 
Sbjct: 236 GGRRNNVNDLKKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFA 295

Query: 245 VNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWV 304
           +NS CH WG R WNT DLS NN  + L A GEGWHNNHHAFE SARHGL+WWE D TW+V
Sbjct: 296 INSVCHKWGGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYV 351

Query: 305 ILFLQALGLATDVKLPSQLHMQKL 328
           I  LQA+GLAT+VKLP++   QKL
Sbjct: 356 IRTLQAIGLATNVKLPTEAQKQKL 351

[Close]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL
Match: A0A6J1GVF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457787 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.8e-174
Identity = 291/331 (87.92%), Postives = 303/331 (91.54%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEATRKE+EDK DKK  FS V  KR  L RKWTTLDRR+A GILFLH+L L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AF LYF TGVLGITLSYHRNLAHRSFKLPKWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHHQFVDTERDPHSPLEGFWFSH+TWMFDSY LT+KIYPK LEDF ERD+KR LS N FV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+ FYRFLRKTYLLHPIALA+VLY  GGL FVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
           A FFVNSACHIWGYRKWNTNDLSKNNWVIGLV+CGEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           TWWVIL LQALGLATDVKLPSQ HMQKLA+Q
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQ 331

[Close]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL
Match: A0A0A0KWV4 (Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.1e-171
Identity = 284/331 (85.80%), Postives = 301/331 (90.94%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEA RKE+EDK+DKK  FS V  KRA   RKWTTLDRR+A GILFLHLL LLAPFH+NW+
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AFALY ITG LGITLSYHRNLAHRSFKLPKWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHHQFVDT+RDPHSP++GFWFSH+TWMFDSY LT KIYPKNLEDF ER++KR LS N FV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALAIVLY  GGL FVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
           A  FVNSACHIWGYRKWNTNDLSKNNW+IGL++ GEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           TWWVIL LQALGLATDVKLP+QLH QKLA+Q
Sbjct: 301 TWWVILLLQALGLATDVKLPTQLHKQKLAIQ 331

[Close]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL
Match: A0A6J1DIZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020970 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.7e-165
Identity = 278/334 (83.23%), Postives = 302/334 (90.42%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEATRKE+ED+ DKKV FS VK +R LLKRKWT LDRRSAG ILFLHLL LLAPFH NW+
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AFALYFITG+ GITLSYHRNLAH+SFKLPKWLEY FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHH+FVDTERDPHSPLEGFWFSH+ WMFD+YALT K+ PKN++DF ERD+KRK  SN+FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQKQAFYRFLRKTY+LHP ALAIVLYA GGL F+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
            TFFVNSACHIWGYR+W+TNDLSKNNW IGL+A GEGWHNNHHAFEYSARHGLQWWEFD 
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDL 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQRHN 334
           TW V+L LQALGLATDVKLPSQ+H QKLAL  HN
Sbjct: 301 TWCVVLVLQALGLATDVKLPSQIHKQKLAL--HN 332

[Close]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 270/334 (80.84%), Postives = 298/334 (89.22%), Query Frame = 0


Query: 1   MEATRKELEDKND-KKVTFS----VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFH 60
           MEATRKELEDKN+ KKV FS    +K +R  LKRKWT LDRRSAGGILFLHLLCLLAPFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWSAFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWV 120
            NW+AFW+AFALYFITG+ GITLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSS 180
           STHRYHHQFVDTERDPHSPLEGFWFSH+TWMFD+Y LT K+ PKN+EDF E+D+KRK  S
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NMFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLI 240
           N+FV  +KYGRP NV+DLQKQAFYRFLR+TY+LHP ALA+VLYA+GGL FVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWW 300
             LH TFF+NSACHIWGYR+WNT DLSKNNWV GL+A GEGWHNNHHAFEYSARHGLQWW
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWW 300

Query: 301 EFDFTWWVILFLQALGLATDVKLPSQLHMQKLAL 330
           EFD TWWV+LFLQ +GLATDVKLPSQ   QKLA+
Sbjct: 301 EFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAM 334

[Close]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL
Match: A0A6J1GCE1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452892 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.6e-157
Identity = 262/320 (81.88%), Postives = 283/320 (88.44%), Query Frame = 0


Query: 11  KNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYF 70
           +N KKV  S VKSK ALL RKWT LDRRSAGG+LFLH LCLLAPFH NW AFW+AFALYF
Sbjct: 2   RNTKKVALSMVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYF 61

Query: 71  ITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTER 130
           ITG+ G+TLSYHRNLAH+SFKLPKWLEY FAYLGVQAFQGDPIDWVSTHRYHHQFVDT+R
Sbjct: 62  ITGLFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDR 121

Query: 131 DPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKN 190
           DPHSPL+GFWFSHL WMFD+Y LT K+YPKN++DF ER +KRK   N+ VG++KYGRP N
Sbjct: 122 DPHSPLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNN 181

Query: 191 VEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACH 250
           VEDLQKQ FYRFLRKTY+LHP A AIVLYA GGL FVVWGMGVRL+ VLH TFFVNSACH
Sbjct: 182 VEDLQKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACH 241

Query: 251 IWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQA 310
           IWGYR W+TNDLSKNNWVIGL+A GEGWHNNHHAFEYSARHG +WWEFD TW VILFLQA
Sbjct: 242 IWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVILFLQA 301

Query: 311 LGLATDVKLPSQLHMQKLAL 330
           LGLATDVKLPS  H QKLAL
Sbjct: 302 LGLATDVKLPSARHQQKLAL 321

[Close]

BLAST of Lcy02g005400 vs. NCBI nr
Match: XP_022955953.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata] >KAG6581939.1 Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 291/331 (87.92%), Postives = 303/331 (91.54%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEATRKE+EDK DKK  FS V  KR  L RKWTTLDRR+A GILFLH+L L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AF LYF TGVLGITLSYHRNLAHRSFKLPKWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHHQFVDTERDPHSPLEGFWFSH+TWMFDSY LT+KIYPK LEDF ERD+KR LS N FV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+ FYRFLRKTYLLHPIALA+VLY  GGL FVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
           A FFVNSACHIWGYRKWNTNDLSKNNWVIGLV+CGEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           TWWVIL LQALGLATDVKLPSQ HMQKLA+Q
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQ 331

[Close]

BLAST of Lcy02g005400 vs. NCBI nr
Match: XP_023528301.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 3.1e-173
Identity = 290/331 (87.61%), Postives = 303/331 (91.54%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEATRKE+EDK DKK  FS V  KR  L RKWTTLDRR+A GILFLH+L L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSMVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AF LYF TGVLGITLSYHRNLAHRSFKLPKWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHHQFVDTERDPHSPLEGFWFSH+TWMFDSY LT+KIYPK +EDF ERD+KR LS N FV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYVEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALA+VLY  GGL FVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
           A FFVNSACHIWGYRKWNTNDLSKNNWVIGLV+CGEGWHNNHHAFEYSARHG+QWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGIQWWEFDF 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           TWWVIL LQALGLATDVKLPSQ HMQKLA+Q
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQ 331

[Close]

BLAST of Lcy02g005400 vs. NCBI nr
Match: XP_004152341.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KGN52907.1 hypothetical protein Csa_014904 [Cucumis sativus])

HSP 1 Score: 610.5 bits (1573), Expect = 8.5e-171
Identity = 284/331 (85.80%), Postives = 301/331 (90.94%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEA RKE+EDK+DKK  FS V  KRA   RKWTTLDRR+A GILFLHLL LLAPFH+NW+
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AFALY ITG LGITLSYHRNLAHRSFKLPKWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHHQFVDT+RDPHSP++GFWFSH+TWMFDSY LT KIYPKNLEDF ER++KR LS N FV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALAIVLY  GGL FVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
           A  FVNSACHIWGYRKWNTNDLSKNNW+IGL++ GEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQ 331
           TWWVIL LQALGLATDVKLP+QLH QKLA+Q
Sbjct: 301 TWWVILLLQALGLATDVKLPTQLHKQKLAIQ 331

[Close]

BLAST of Lcy02g005400 vs. NCBI nr
Match: XP_022153467.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-164
Identity = 278/334 (83.23%), Postives = 302/334 (90.42%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFS-VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWS 60
           MEATRKE+ED+ DKKV FS VK +R LLKRKWT LDRRSAG ILFLHLL LLAPFH NW+
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFW+AFALYFITG+ GITLSYHRNLAH+SFKLPKWLEY FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFV 180
           YHH+FVDTERDPHSPLEGFWFSH+ WMFD+YALT K+ PKN++DF ERD+KRK  SN+FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQKQAFYRFLRKTY+LHP ALAIVLYA GGL F+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 ATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDF 300
            TFFVNSACHIWGYR+W+TNDLSKNNW IGL+A GEGWHNNHHAFEYSARHGLQWWEFD 
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDL 300

Query: 301 TWWVILFLQALGLATDVKLPSQLHMQKLALQRHN 334
           TW V+L LQALGLATDVKLPSQ+H QKLAL  HN
Sbjct: 301 TWCVVLVLQALGLATDVKLPSQIHKQKLAL--HN 332

[Close]

BLAST of Lcy02g005400 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 581.6 bits (1498), Expect = 4.2e-162
Identity = 270/334 (80.84%), Postives = 298/334 (89.22%), Query Frame = 0


Query: 1   MEATRKELEDKND-KKVTFS----VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFH 60
           MEATRKELEDKN+ KKV FS    +K +R  LKRKWT LDRRSAGGILFLHLLCLLAPFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWSAFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWV 120
            NW+AFW+AFALYFITG+ GITLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSS 180
           STHRYHHQFVDTERDPHSPLEGFWFSH+TWMFD+Y LT K+ PKN+EDF E+D+KRK  S
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NMFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLI 240
           N+FV  +KYGRP NV+DLQKQAFYRFLR+TY+LHP ALA+VLYA+GGL FVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWW 300
             LH TFF+NSACHIWGYR+WNT DLSKNNWV GL+A GEGWHNNHHAFEYSARHGLQWW
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWW 300

Query: 301 EFDFTWWVILFLQALGLATDVKLPSQLHMQKLAL 330
           EFD TWWV+LFLQ +GLATDVKLPSQ   QKLA+
Sbjct: 301 EFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAM 334

[Close]

BLAST of Lcy02g005400 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 404.4 bits (1038), Expect = 8.6e-113
Identity = 181/308 (58.77%), Postives = 225/308 (73.05%), Query Frame = 0


Query: 21  KSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLSY 80
           K K    +R+W  +D  +   +L +HLL LLAPF +NW A  +AF LY +TG+LGITLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 81  HRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWF 140
           HRNL+H++FKLPKWLEY FAY G QA QG+PIDWVSTHRYHHQF D++RDPHSPL+GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 141 SHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFYR 200
           SH+ WMFD+  +T +                             G P NV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 201 FLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTND 260
           FLR TY+LHP+ALA+ LYA+GG  F+VWGMGVR++ V H T+ VNSACH+WG + WNT D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 327

Query: 261 LSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLPS 320
           LSKNNW +  +A GEGWHNNHHAFE+SARHGL+WW+ D TW+V+ FLQA+GLATDVKLPS
Sbjct: 328 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPS 367

Query: 321 QLHMQKLA 329
           +   Q++A
Sbjct: 388 EAQKQRMA 367

[Close]

BLAST of Lcy02g005400 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-96
Identity = 168/334 (50.30%), Postives = 216/334 (64.67%), Query Frame = 0


Query: 1   MEATRKELEDKNDKKVTFSV---KSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYN 60
           M  +  E E+ N K         + KRA+ +RKW  LD   A   LF+H LCLLAPF++ 
Sbjct: 1   MSLSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFT 60

Query: 61  WSAFWIAFALYFITGVLGITLSYHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVST 120
           W A  +A  +Y + G LGIT+SYHRNLAHRSFK+PKWLEY FAY G+ A QGDPIDWVST
Sbjct: 61  WPALRVALIVYTVGG-LGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVST 120

Query: 121 HRYHHQFVDTERDPHSPLEGFWFSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNM 180
           HRYHHQF D++RDPHSP EGFWFSHL W+FD+  L  K                      
Sbjct: 121 HRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC--------------------- 180

Query: 181 FVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICV 240
                  GR  NVEDL++Q +Y+FL++T L H +    +LY  GGLSF+ WGMG+ +   
Sbjct: 181 -------GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAME 240

Query: 241 LHATFFVNSACHIWGYRKWNTNDLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEF 300
            H T  +NS CH+WG R W TND S+N W + + + GE WHNNHHAFE SAR GL+WW+ 
Sbjct: 241 HHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQI 300

Query: 301 DFTWWVILFLQALGLATDVKLPSQLHMQKLALQR 332
           D +W+++ FL+ +GLATDVKLPS+   +++A+ R
Sbjct: 301 DISWYIVRFLEIIGLATDVKLPSESQRRRMAMVR 305

[Close]

BLAST of Lcy02g005400 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 349.0 bits (894), Expect = 4.3e-96
Identity = 162/310 (52.26%), Postives = 211/310 (68.06%), Query Frame = 0


Query: 20  VKSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLS 79
           V++KR+  +R WT+ D       +  HLL LLAPF+++W AFW+   L FI G+  ITLS
Sbjct: 80  VRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLS 139

Query: 80  YHRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFW 139
           YHRNL+HRSF LPKWLEY FAY GV AFQGDPI+WVS HRYHH+  +T+RDPHSP +GFW
Sbjct: 140 YHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFW 199

Query: 140 FSHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFY 199
           FSH+ W+FD+ ++                                G  +NV+DL +Q FY
Sbjct: 200 FSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPFY 259

Query: 200 RFLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTN 259
           RFL++T LLH +A + + Y  GG+  +VWG+G+ +   LH TF VNS CHIWG R WNT+
Sbjct: 260 RFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTS 319

Query: 260 DLSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLP 319
           D SKNNW + ++  GEGWHNNHHAFE+SARHGL+WW+ D TW +I FL+A+GLAT+VKLP
Sbjct: 320 DFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLP 360

Query: 320 SQLHMQKLAL 330
           ++  M+  AL
Sbjct: 380 TETQMKGKAL 360

[Close]

BLAST of Lcy02g005400 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 342.4 bits (877), Expect = 4.0e-94
Identity = 159/303 (52.48%), Postives = 198/303 (65.35%), Query Frame = 0


Query: 29  RKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLSYHRNLAHRS 88
           R+W  LD         +H L LLAPF++ WSA W+ F  Y I G LGIT+SYHRNLAHRS
Sbjct: 34  RRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG-LGITVSYHRNLAHRS 93

Query: 89  FKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHLTWMFD 148
           FK+PKWLEY  AY  + A QGDPIDWVSTHRYHHQF D+ERDPHSP EGFWFSHL W++D
Sbjct: 94  FKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYD 153

Query: 149 SYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLL 208
           S  L  K                             GR  NVEDL++Q FYRFL+KT L 
Sbjct: 154 SAYLVSKC----------------------------GRRANVEDLKRQWFYRFLQKTVLF 213

Query: 209 HPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTNDLSKNNWVI 268
           H + L   L+ +GG+SFV WGMGV     +H T  +NS CHIWG R W TND S+N W +
Sbjct: 214 HILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWL 273

Query: 269 GLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLPSQLHMQKLA 328
            + + GE WHNNHHAFE SAR GL+WW+ D +W+++ F + +GLATDVK+P++   +++A
Sbjct: 274 SVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 307

Query: 329 LQR 332
           + R
Sbjct: 334 IVR 307

[Close]

BLAST of Lcy02g005400 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-90
Identity = 163/311 (52.41%), Postives = 200/311 (64.31%), Query Frame = 0


Query: 21  KSKRALLKRKWTTLDRRSAGGILFLHLLCLLAPFHYNWSAFWIAFALYFITGVLGITLSY 80
           K KRA L RKWT +D      +  +HLLCLLAPF+Y W AF  A A+  I+  L IT SY
Sbjct: 18  KEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFA-AMVGISTNLSITFSY 77

Query: 81  HRNLAHRSFKLPKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWF 140
           HRNL HRSFKLPKWLEY FAY  + A QG PIDWVSTHR+HHQF D++RDPHSP+EGFWF
Sbjct: 78  HRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWF 137

Query: 141 SHLTWMFDSYALTHKIYPKNLEDFAERDSKRKLSSNMFVGFLKYGRPKNVEDLQKQAFYR 200
           SH+ W+FD+  +  K                             G   NV DL++Q FYR
Sbjct: 138 SHVFWIFDTSYIREKC----------------------------GGRDNVMDLKQQWFYR 197

Query: 201 FLRKTYLLHPIALAIVLYAIGGLSFVVWGMGVRLICVLHATFFVNSACHIWGYRKWNTND 260
           FL+ T  LH +   I++Y  GGL ++ W +GV      HAT+ +NSACHIWG R WNT D
Sbjct: 198 FLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWNTKD 257

Query: 261 LSKNNWVIGLVACGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILFLQALGLATDVKLPS 320
            S+N W +G    GE WHNNHHAFE SARHGL+W++ D TW++I F Q LGLATDVKLP+
Sbjct: 258 TSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATDVKLPT 299

Query: 321 QLHMQKLALQR 332
               +K++L R
Sbjct: 318 DAQKRKMSLAR 299

The following BLAST results are available for this feature:

BLAST of Lcy02g005400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	1.2e-111	58.77	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	1.6e-95	50.30	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
Q9LVZ3	6.0e-95	52.26	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P0DOW2	1.0e-94	50.00	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9FV68	3.0e-94	53.40	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]

BLAST of Lcy02g005400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GVF3	8.8e-174	87.92	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KWV4	4.1e-171	85.80	Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1	[more]
A0A6J1DIZ7	5.7e-165	83.23	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DIX0	2.0e-162	80.84	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GCE1	2.6e-157	81.88	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of Lcy02g005400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022955953.1	1.8e-173	87.92	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_023528301.1	3.1e-173	87.61	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_004152341.2	8.5e-171	85.80	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi...	[more]
XP_022153467.1	1.2e-164	83.23	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153442.1	4.2e-162	80.84	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]

BLAST of Lcy02g005400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	8.6e-113	58.77	fatty acid desaturase 5	[more]
AT1G06080.1	1.1e-96	50.30	delta 9 desaturase 1	[more]
AT3G15870.1	4.3e-96	52.26	Fatty acid desaturase family protein	[more]
AT2G31360.1	4.0e-94	52.48	16:0delta9 desaturase 2	[more]
AT1G06120.1	1.2e-90	52.41	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 58..80 score: 24.71 coord: 118..147 score: 49.12 coord: 81..101 score: 33.33
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 23..165
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 183..331
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 58..317 e-value: 5.32705E-76 score: 229.365
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 61..303 e-value: 2.6E-17 score: 63.5
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 183..331
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 23..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g005400.1	Lcy02g005400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042149	cellular response to glucose starvation
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031588	nucleotide-activated protein kinase complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016208	AMP binding
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function	GO:0019901	protein kinase binding
molecular_function	GO:0019887	protein kinase regulator activity