Lcy02g005020 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g005020
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Ribonuclease 3-like protein 1
Location	Chr02: 6592659 .. 6595839 (-)
RNA-Seq Expression	Lcy02g005020
Synteny	Lcy02g005020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTATTCAGAAGAGGTCGCTTCCAGAGGCCCAGAATCTATTCAGAGAAACTTCGCCATTTTTTTGGTATGAGATCTGTCCATTTTGCTCTGAATCTTCCATTGCTGATTTCGCATTCAAATTGATGTATGACACTCTGCAGAGAGATCATTGGACCCGAGCGGAAGAATCGAGGAAAGTAAATGGAGAATACTCCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATTAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCGATTCCGGTAAAACTTGGTTGTTTTCCCGCGACTGTGTTCGATTTTCTTTGGAATTCGAAAGGAATTTGATGTTGAGTTGAAAATTTTCAGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTATCGATTCAAACCTAGTTCCTCAACTTGATTGCTGTTTGGAATTCGAAATAGATGATTCCTGTGATTTTTCATCATTTTCATGGATTATTCTTTTCCTTTTCTTCGTTATTTGCTGGTTTTCTTCTAATTGATTGATTTGAAAATGCAATTGAATGTTCATCTCACTTGCATTCCATCAGGAGCAGTCTTTATCGCCCATTTCTCCCCAATCCCTCTCCCACTTCGTCGTCATTAGCATTCGCCAATAATTTTAACGTGCAGTTACAAACCCAAAAAAGAACAAAGAAAAAAGAAAAAAAAAGGGGGGGGAGGGGGTTTGCTATGGTAAGTATTTGATCTCAACACTTCCAAAACTGAACTCTGAATTATTTCCTTGATTGTGTTTGAAGATTATCATTACTTTCCTCCCTTTCTTATTAGAGAGCGGGATGGATGTTACCATCTATATCATTTTTTTTTCTTGTTTGTCTTCAATTCTTCTTAGCTGACTTGTTTTCTAAAAGAAATGAAGTTGTACGTGAATTTTGTATTCCAACCATACAATAACAGTAACATTATAAATTATCCGATCGAAATCTTTAGTGGCTGAAAGTTGTGAGAATAGTTATCTAAAGTAGGCCATTAGTTTCTTAAAATAACAAGTTACTCGTCAACCCTCAAGGGGTGCCCTGCACGTAAGAGCTGGAATCTCAAAATCCCACTTCAAATATGTAAATAGAAAACTTAGGCCGAGTATGATAACTATTTGGTTTTTGAAAATTGTGTTTGTTTTCTCTCAATTTTCTTTCGACGGTTTGCGTTTTATTATGAAACATTTAAATTTTTAGTCATATTCCAAAAAAAAAAAAAACAAGTTTTTTGAAAACTACTTTTTAAGTTTCAAAACTTGATTTGTTTTTAAAACATGTGAAGAAGGTAGATAACAAAACAAATAAATATAAGTGGAAGTATGGTTTATAAACTTAATTTTCAAAAACAGAAAATAAAATAGTCATCAAATCTACCTTAATATTTAAAATCTTTGTTGTCTTTTAGAGTTGACTTTGGAGCTGAGCTCCTTCGAGACTAATGGAGTGGGTCCCATTCTCCAGTTACCAATTAAAAGAAAAAACTCAGGAATAAAGTAAGACAAAACTAACTTAGAAATCGGGTACTCAAACATGTTTATACCCTTTTTTGTTTGAAAATCGAAAGTGATTTTGAAACTCAACTACCAAAAAGCACATAGGCTCTTGCACTTTGATGGCTTACTCGAGTTTTATCGACACTTTATTTCATTTAACTTTCTGCCTTCCTTATCTCAGTTTGAGTACATTGGCTTTTACAATTATACTGCTTATTCTGATTCCCATAAAATTTACCATGGCGAAATTTCCATTTTGGGTATTCAAAGTTTGCTTTAATGGTACAACTTCACCTTTCCATTTTAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTAAGCTCAATGACTAATCATCAGTAAGCGTTAGTAGTTATGAAGTACTAACACTTAATTCCAGTAACATATAGTGCCAAACTTTCAACACCCTCACAAAAGTGAAGGTATATTTTGGAGTGATTTTGAACATGACAAAAGTGATTTTAACTATTTCAAAATAACTTACGAACATGTTCTTGGTCCTCGTCTTGTGGATTACTCGCTAAAACTCAGAAAAATTTCCCTTCTAACACCTACTTTTGGAAGCTTTTCTAACGTTTCTGCCTTTTACCTTCAAAATGGGGATAACTAGATGGCACCATGTATGTTTTTGAGAAGGTGAGGGGTTACCAAGAGAGAGCTATTCAAGTTTCTTTTTACTTCATTGTTTGGATCTGACATGTTCATAGCCATCTGTGTGCAAACATGAGCTATAGCATAGATTGGAGAGTATTGATTTCCAGACAGGATTCAATAGAAGCGTTATTTAATGGGATTTTTGCAACTGATGTAAATTTGATTTTGATTTAGGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCACAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAATCAGGAATTGTAAAGAACTTGAAATGGAAAGTGAATAAGCTATTTAAACACAAGACTTTTTTGTGGAATAATATTTTCATTAACATGTAATCCGTGGGACCCATAATAAAGTGTTGGAATAAGAGTTAAAAAGTTTGTAGGACCAACAATAGAGTATTGTGATTGGTTGATAAATTTGTGGGTCCCACAATTGGATGTTGTATATCCTTGGGCCAAACAATTAACTTCACCCAACTCCAACTTCACAACTCATTGCCCCAAACAATTGGATGTTTGGTTTAAGGTTTTATAGATGCCTGAGAATACATGATGCCTGAGAATACTATGCCTGGGAAGATAATTGTGTTTGGTTGTGCATGGGAATGTCATCAGGA

mRNA sequence

GATTTATTCAGAAGAGGTCGCTTCCAGAGGCCCAGAATCTATTCAGAGAAACTTCGCCATTTTTTTGAGAGATCATTGGACCCGAGCGGAAGAATCGAGGAAAGTAAATGGAGAATACTCCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATTAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCGATTCCGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCACAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAATCAGGAATTGTAAAGAACTTGAAATGGAAAGTGAATAAGCTATTTAAACACAAGACTTTTTTGTGGAATAATATTTTCATTAACATGTAATCCGTGGGACCCATAATAAAGTGTTGGAATAAGAGTTAAAAAGTTTGTAGGACCAACAATAGAGTATTGTGATTGGTTGATAAATTTGTGGGTCCCACAATTGGATGTTGTATATCCTTGGGCCAAACAATTAACTTCACCCAACTCCAACTTCACAACTCATTGCCCCAAACAATTGGATGTTTGGTTTAAGGTTTTATAGATGCCTGAGAATACATGATGCCTGAGAATACTATGCCTGGGAAGATAATTGTGTTTGGTTGTGCATGGGAATGTCATCAGGA

Coding sequence (CDS)

ATGGAGAATACTCCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATTAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCGATTCCGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCACAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAA

Protein sequence

MENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAQHAAEGALWYLKHLGYRSKR

Homology

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BLAST of Lcy02g005020 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.3e-24
Identity = 47/89 (52.81%), Postives = 69/89 (77.53%), Query Frame = 0


Query: 108  GNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYG 167
            G+   + A+S LFE+C AN+W+PP F+ C+EEGPSH++K+ +KV+VE+KGAS  +LEC+ 
Sbjct: 1563 GDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGASATLLECHS 1622

Query: 168  NPQTRKKVAAQHAAEGALWYLKHLGYRSK 197
            + + +KK A +HAA+GALW LK LG+  K
Sbjct: 1623 DGKLQKKAAQEHAAQGALWCLKQLGHLPK 1651

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BLAST of Lcy02g005020 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-23
Identity = 48/88 (54.55%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 106  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 165
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 166  YGNPQTRKKVAAQHAAEGALWYLKHLGY 194
            YG  +  KK AA+HAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

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BLAST of Lcy02g005020 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-20
Identity = 60/187 (32.09%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 9   RKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSA 68
           R + + LK++PP+        + SSIP     ++    V        + + K  E  ++ 
Sbjct: 15  RSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNV-GMQRFQEKTDFKFEEEDNAI 74

Query: 69  RALNTKTIPEASSDSFDKKKE---NSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTA 128
            + +   I   S+ S   +K+      E  +T +       E  + + +AKS+L E+C +
Sbjct: 75  SSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDESKTRRGSAKSVLHEMCAS 134

Query: 129 NHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGAS-EAVLECYGNPQTRKKVAAQHAAEGA 188
             W+PP++ECC  +GP H++ + +KV+VE++ +S + VLEC+G+P+ +KK AA+HAAEGA
Sbjct: 135 KRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEHAAEGA 192

Query: 189 LWYLKHL 192
           LWYL+H+
Sbjct: 195 LWYLEHV 192

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BLAST of Lcy02g005020 vs. ExPASy TrEMBL
Match: A0A6J1DJ12 (uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020939 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.5e-80
Identity = 157/194 (80.93%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRS-SIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           ME TP YPR+LNV+LKNLPPIN  R GNGN   SIP KFEKSRYIRRVP F PPNHRPEM
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINP-RTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEM 60

Query: 61  KPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSLL 120
           KP    D+A ALNTKTIPEASSDSF  K ENSREIASTCSCGH S KEG  EKRAAKSLL
Sbjct: 61  KP----DTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLL 120

Query: 121 FEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAQH 180
           FE+CTAN+W+PPLFECCEEEGPSH KK+RFKVIVEMKGA +AVLECYGN Q RKKVAAQ+
Sbjct: 121 FEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQN 180

Query: 181 AAEGALWYLKHLGY 194
           AAEGALW LKHLGY
Sbjct: 181 AAEGALWCLKHLGY 189

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BLAST of Lcy02g005020 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.9e-76
Identity = 152/209 (72.73%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MENTPSY-PRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           MEN PS  P +LN++LKNLPPINN R  +G+ SSIP KFEKSRYIRRVPQFMP +HRPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  KPNE---------YSDSARALNTKTIPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEG 120
           + N+         YSDSARALNTKT P A S+DSF +KK+  +++AS CS C H STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 NSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGN 180
             EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV +EMKG  EAV+ECYGN
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGN 198

Query: 181 PQTRKKVAAQHAAEGALWYLKHLGYRSKR 198
            QTRKKVAA+HAAEGALWYL HLGYR KR
Sbjct: 199 LQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

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BLAST of Lcy02g005020 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 9.5e-75
Identity = 147/208 (70.67%), Postives = 169/208 (81.25%), Query Frame = 0


Query: 2   ENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKP 61
           +N PS P +LN++LKNLPPINN R   G  SSIP KFEKSRYIRRVPQF+P +HRPE++ 
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEIRS 80

Query: 62  NEY----------SDSARALNTKTIPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEGN 121
           N Y          SDSARA+NTKTIP A S+D F +KK+N +++AS CS C HGSTKEG 
Sbjct: 81  NNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEGT 140

Query: 122 SEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNP 181
            EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH +KYRFKV +EMKG  EAV+ECYGN 
Sbjct: 141 PEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYGNL 200

Query: 182 QTRKKVAAQHAAEGALWYLKHLGYRSKR 198
           QTRKK+AA+HAAEGALWYLK+LGYR +R
Sbjct: 201 QTRKKMAAEHAAEGALWYLKNLGYRFER 228

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BLAST of Lcy02g005020 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 9.5e-75
Identity = 147/208 (70.67%), Postives = 169/208 (81.25%), Query Frame = 0


Query: 2   ENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKP 61
           +N PS P +LN++LKNLPPINN R   G  SSIP KFEKSRYIRRVPQF+P +HRPE++ 
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEIRS 80

Query: 62  NEY----------SDSARALNTKTIPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEGN 121
           N Y          SDSARA+NTKTIP A S+D F +KK+N +++AS CS C HGSTKEG 
Sbjct: 81  NNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEGT 140

Query: 122 SEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNP 181
            EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH +KYRFKV +EMKG  EAV+ECYGN 
Sbjct: 141 PEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYGNL 200

Query: 182 QTRKKVAAQHAAEGALWYLKHLGYRSKR 198
           QTRKK+AA+HAAEGALWYLK+LGYR +R
Sbjct: 201 QTRKKMAAEHAAEGALWYLKNLGYRFER 228

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BLAST of Lcy02g005020 vs. ExPASy TrEMBL
Match: A0A6J1GWD5 (ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111458030 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.3e-68
Identity = 136/203 (67.00%), Postives = 155/203 (76.35%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMK 60
           MENTPS PR +NV+LKNLPPI+N  +GN N SSIP KFEKSRYI RVPQFMPPNH P+++
Sbjct: 1   MENTPSCPRSVNVNLKNLPPIHNPCVGNANHSSIPAKFEKSRYIGRVPQFMPPNHSPDVR 60

Query: 61  PNEYS---------DSARALNTKTIPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNS 120
           PNEY          DSAR+ N +T P+       ++KEN  + AS  S C H STKEG S
Sbjct: 61  PNEYDGVPMNPACFDSARSPNARTTPK-------ERKENFGDSASRSSGCSHDSTKEGTS 120

Query: 121 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQ 180
            K++AKSLL+EICTANHW+PPLFECCEEEGPSH KKY FKVIVEMKG     LECYG PQ
Sbjct: 121 VKKSAKSLLYEICTANHWKPPLFECCEEEGPSHAKKYTFKVIVEMKGDCGEDLECYGKPQ 180

Query: 181 TRKKVAAQHAAEGALWYLKHLGY 194
           TRKKVAA+ AAEGA+WYLKHLGY
Sbjct: 181 TRKKVAAEDAAEGAVWYLKHLGY 196

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BLAST of Lcy02g005020 vs. NCBI nr
Match: XP_022153429.1 (uncharacterized protein LOC111020939 isoform X1 [Momordica charantia])

HSP 1 Score: 308.9 bits (790), Expect = 3.1e-80
Identity = 157/194 (80.93%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRS-SIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           ME TP YPR+LNV+LKNLPPIN  R GNGN   SIP KFEKSRYIRRVP F PPNHRPEM
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINP-RTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEM 60

Query: 61  KPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSLL 120
           KP    D+A ALNTKTIPEASSDSF  K ENSREIASTCSCGH S KEG  EKRAAKSLL
Sbjct: 61  KP----DTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLL 120

Query: 121 FEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAQH 180
           FE+CTAN+W+PPLFECCEEEGPSH KK+RFKVIVEMKGA +AVLECYGN Q RKKVAAQ+
Sbjct: 121 FEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQN 180

Query: 181 AAEGALWYLKHLGY 194
           AAEGALW LKHLGY
Sbjct: 181 AAEGALWCLKHLGY 189

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BLAST of Lcy02g005020 vs. NCBI nr
Match: XP_038878793.1 (ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 306.6 bits (784), Expect = 1.5e-79
Identity = 160/204 (78.43%), Postives = 170/204 (83.33%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE-- 60
           MENT S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP   
Sbjct: 1   MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 60

Query: 61  ----MKPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEKR 120
               MKP   S SARALN+KTIPEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EKR
Sbjct: 61  AGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKR 120

Query: 121 AAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRK 180
           AAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTRK
Sbjct: 121 AAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRK 180

Query: 181 KVAAQHAAEGALWYLKHLGYRSKR 198
           KVAA+HAAEGA+WYLK+LGY  KR
Sbjct: 181 KVAAEHAAEGAIWYLKNLGYCFKR 203

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BLAST of Lcy02g005020 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 306.6 bits (784), Expect = 1.5e-79
Identity = 160/204 (78.43%), Postives = 170/204 (83.33%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE-- 60
           MENT S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP   
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 77

Query: 61  ----MKPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEKR 120
               MKP   S SARALN+KTIPEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EKR
Sbjct: 78  AGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKR 137

Query: 121 AAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRK 180
           AAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTRK
Sbjct: 138 AAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRK 197

Query: 181 KVAAQHAAEGALWYLKHLGYRSKR 198
           KVAA+HAAEGA+WYLK+LGY  KR
Sbjct: 198 KVAAEHAAEGAIWYLKNLGYCFKR 220

[Close]

BLAST of Lcy02g005020 vs. NCBI nr
Match: XP_004152191.1 (uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical protein Csa_014441 [Cucumis sativus])

HSP 1 Score: 294.7 bits (753), Expect = 6.1e-76
Identity = 152/209 (72.73%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MENTPSY-PRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           MEN PS  P +LN++LKNLPPINN R  +G+ SSIP KFEKSRYIRRVPQFMP +HRPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  KPNE---------YSDSARALNTKTIPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEG 120
           + N+         YSDSARALNTKT P A S+DSF +KK+  +++AS CS C H STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 NSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGN 180
             EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV +EMKG  EAV+ECYGN
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGN 198

Query: 181 PQTRKKVAAQHAAEGALWYLKHLGYRSKR 198
            QTRKKVAA+HAAEGALWYL HLGYR KR
Sbjct: 199 LQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of Lcy02g005020 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 292.0 bits (746), Expect = 3.9e-75
Identity = 152/193 (78.76%), Postives = 161/193 (83.42%), Query Frame = 0


Query: 1   MENTPSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE-- 60
           MENT S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP   
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 77

Query: 61  ----MKPNEYSDSARALNTKTIPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEKR 120
               MKP   S SARALN+KTIPEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EKR
Sbjct: 78  AGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKR 137

Query: 121 AAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRK 180
           AAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTRK
Sbjct: 138 AAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRK 197

Query: 181 KVAAQHAAEGALW 187
           KVAA+HAAEGA+W
Sbjct: 198 KVAAEHAAEGAIW 209

[Close]

BLAST of Lcy02g005020 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-24
Identity = 48/88 (54.55%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 106  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 165
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 166  YGNPQTRKKVAAQHAAEGALWYLKHLGY 194
            YG  +  KK AA+HAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Lcy02g005020 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-24
Identity = 48/88 (54.55%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 106  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 165
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1658

Query: 166  YGNPQTRKKVAAQHAAEGALWYLKHLGY 194
            YG  +  KK AA+HAA+ A+W LKH G+
Sbjct: 1659 YGEARATKKGAAEHAAQAAIWCLKHSGF 1686

[Close]

BLAST of Lcy02g005020 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 101.3 bits (251), Expect = 9.2e-22
Identity = 60/187 (32.09%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 9   RKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSA 68
           R + + LK++PP+        + SSIP     ++    V        + + K  E  ++ 
Sbjct: 15  RSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNV-GMQRFQEKTDFKFEEEDNAI 74

Query: 69  RALNTKTIPEASSDSFDKKKE---NSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTA 128
            + +   I   S+ S   +K+      E  +T +       E  + + +AKS+L E+C +
Sbjct: 75  SSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDESKTRRGSAKSVLHEMCAS 134

Query: 129 NHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGAS-EAVLECYGNPQTRKKVAAQHAAEGA 188
             W+PP++ECC  +GP H++ + +KV+VE++ +S + VLEC+G+P+ +KK AA+HAAEGA
Sbjct: 135 KRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEHAAEGA 192

Query: 189 LWYLKHL 192
           LWYL+H+
Sbjct: 195 LWYLEHV 192

[Close]

BLAST of Lcy02g005020 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 47/90 (52.22%), Postives = 64/90 (71.11%), Query Frame = 0


Query: 105 TKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASE-AVL 164
           T E  +++ +AKS L+ +C+  HW+ PL+E    EGP H+K +  KV VEMK  S   VL
Sbjct: 75  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 134

Query: 165 ECYGNPQTRKKVAAQHAAEGALWYLKHLGY 194
           EC+GNPQ +KK+AA+ AAE ALWYLK++GY
Sbjct: 135 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 163

[Close]

BLAST of Lcy02g005020 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 47/90 (52.22%), Postives = 64/90 (71.11%), Query Frame = 0


Query: 105 TKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASE-AVL 164
           T E  +++ +AKS L+ +C+  HW+ PL+E    EGP H+K +  KV VEMK  S   VL
Sbjct: 53  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 112

Query: 165 ECYGNPQTRKKVAAQHAAEGALWYLKHLGY 194
           EC+GNPQ +KK+AA+ AAE ALWYLK++GY
Sbjct: 113 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 141

The following BLAST results are available for this feature:

BLAST of Lcy02g005020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7LFZ6	3.3e-24	52.81	Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
P84634	1.6e-23	54.55	Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2	[more]
Q9M8N2	1.3e-20	32.09	Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1	[more]

BLAST of Lcy02g005020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ12	1.5e-80	80.93	uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0KYI7	2.9e-76	72.73	DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S...	[more]
A0A5D3E0Z9	9.5e-75	70.67	Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXK0	9.5e-75	70.67	uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1GWD5	2.3e-68	67.00	ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Lcy02g005020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153429.1	3.1e-80	80.93	uncharacterized protein LOC111020939 isoform X1 [Momordica charantia]	[more]
XP_038878793.1	1.5e-79	78.43	ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida]	[more]
XP_038878769.1	1.5e-79	78.43	ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib...	[more]
XP_004152191.1	6.1e-76	72.73	uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical ...	[more]
XP_038878786.1	3.9e-75	78.76	ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Lcy02g005020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20320.1	1.2e-24	54.55	dicer-like 4	[more]
AT5G20320.2	1.2e-24	54.55	dicer-like 4	[more]
AT1G80650.1	9.2e-22	32.09	RNAse THREE-like protein 1	[more]
AT4G00420.2	1.0e-20	52.22	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]
AT4G00420.3	1.0e-20	52.22	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 115..189 e-value: 5.4E-10 score: 49.2
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 114..190 score: 9.513474
None	No IPR available	GENE3D	3.30.160.20		coord: 121..190 e-value: 1.5E-22 score: 81.9
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 115..189 e-value: 3.6E-14 score: 52.8
None	No IPR available	PANTHER	PTHR14950:SF67	DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEIN	coord: 14..197
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 14..197
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 115..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g005020.1	Lcy02g005020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding