Lcy02g004290 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g004290
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCytochrome P450
LocationChr02: 5683697 .. 5688642 (-)
RNA-Seq ExpressionLcy02g004290
SyntenyLcy02g004290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAGATTCCCACTACATTCTCTACATTGCTCTCTTCTTCGTCGTCTTCACCATTCTCCTTCAATATCTCCTTCACAGACCTTCAAAGAGCCTCACCCATGGCCGCCGCCGGCCGCCAAGTCCGCCGGCGCTGCCACTCATCGGCCACCTCCACTACTTCTCGCCGTACGTCAGCAAATCGTTCCACAATCTCGCCACCCGGTACGGCGACCTCCTCCTCCTCCGGCTAGGCAGCATCCAATGCGTCGTCGTTTCGTCGGCGGCGTACGCGGCGGAGGTCTACAAGAATCAGGACGTTATGTTCTCGTCGCGGCCGAAATTCGCGTTCGCCGACGAGCTGCCGTACGCGAAGGCCGGATTCTTCGGGGCGGAGTACGGGAGTTACTGGAGATATATGAAGAAACTGACGATGACGGAGCTTTTATCGCAGCGGCAAGTTGAGCGATCACGCGGCGTACGGAGAGAAGAAATGTTGAAATTCTTGAGGAAATTGGCGGATTCCGGCGAGAAGAACGAGGCTCTGGATTTGGGCGCCGAGCTCATCAAACTCACCAACAATAGTATTTGTAGGTAAGAACTTAATATTTTTTCATGCTCAAATTAAATCATCCAAAAAATGTTTTCAAAAAAAAAAAAAAAAATCATCCAAAAATATTTAAAATTTTAGACCCGTTCGATAATCATTTGGTTTCTGAAAACTAAACTTTAATGAGTTTTTTTTTTTTGTTACTTTCCATGAATATTTTAAAAAATCAAGTTCAATTCGATTTTAAAATTTTTTTTTTTTTTTTTTTGAAATTCGACAAGAAAAGGTGACAAAGATAGTGAAGAAATGATAAAGAAACCATACTAAATTTCAAAAACAGAAAAACAAAAATCAAACTAATTAGTTCGTTTTTTAAAACTTGTTTTTGCTTTTGAAATACGGTAAGAAAGGTGACAAAAGACAGTAAAGAATTTGTAAACAAAGGAACATAAATTTCAAAAACAGAAAAAATAAAAATGAAATTATGATCAAACGAGATTTTAAAGGTTTAAATTCGATTTTGGTGTTTATTCTAATTTCTACTTTTATTAATTTTCAAGCATTTTATTTTGATATTTTTACATTTAGCTTTTGTCCCTTTTGGTCTTTGTACCTTTGAAATTTCTGATTTGGTCTTTGTCCTTTTTAAAAAATAACTATTTTTTGTTGTTAGCACAATTGAAACATGAATACTATATACATAATGATAATTCTTTAATACAAAATTATGATTAAAAAAATTGAATGAGTATAGTGTTGTGTTGAATGACGATTTGTAAAGAATGAAATATATACATAAAGAAAACTAAAGGACTAAAATATTCACTTTTTAGAAGTACAAGAAATTGGAATCGAATAAAATGAACATTTTGATATATACTGGGACGAAAATTGAAAAAGCTAAAAGTACAGTGATCAAATAAACATTTTGAAAGTTTATGGAGACCAAAATCAACCAAAACGGAAAGTTGAGGAACTAAAATAAGATTTAAACTAAAAAAAAATAACGACAAAAATGGTAAAGTTTTAAAAAGTTTAACGGGTGTTTCAAAAGTGTAGGAACTAGCATGGACAAATTTTGAAACTACAAAAGATTAGGATGGGTATTTTGAAATGATTATCAAAAACAAAAATACAGAAAGACTATAGACCAAAATAAAAAAAATTAAGTTGGTTTGATAACCATTGTTTTTTGAAATCAGACTTTTTTTCTCCAATTTTTTTTTATTATGTTTTCATTTTTTTTTAATAAGAATACATTTGAATTTCTAACCAAATTCCAAAAACCAAAAGAAGTTTTTGAAAACTATTTTTTTGTAGTTTCAAAACCTGACTTGATTTTTGAAAACATAAGAAAAAAATAGATAACAAAATAAAGGAAATATAGAAGCAATATTTATAAGCTTAATTTTCAAAAATAAAAAACCAAAAGTCAAATGGTTATCTGAACCTTAATAAAAAATCACTCTAAGGTGAAAAAAATTGATAAATAATTCTAAATATGTTTGTCGATAACTTCTAGGCTTCTAATAACAATTAACTATGATAGGCGAAAGAATCTTTTGAACATGTCAAAGAATAATGAAGATATGTCGATTTGTTACCATACAATTTTTGTAAAATATTTTATTTAAGTATTCATACTAAATTAAAATTGTCTCACCTTTATTTATTTATTTATTTATATATATATAGGACAGTAGATATTTGACATCTTCTTTTCACTTTTTCTTTTTAGTTTAATACGTCTTATCTTTTTGTCAATGATTAATAGTTTAACTAGTTGGATATATTCTCTTCTTTTGAAAATTTTGTCCACACAAATTAGAGGAAAATTGCAGTAAATAATATGGAAAAAGATCAAAAAATTACTTAAGATTTGAATATACTTTTCTATGTTTTTTTTTTTACTATACTTAAGATTTGAATATATTTTTCTATGTTCCTAGTAAAAAAAATTAATGAATTAGGCTTTTGAAATCTATATTTTATTTCCTGAAATATAGTAAAGATTTCAAAAAGAATTTTGAAGTAGTAAAGAAAGTATAAAAACTATTAATAAATAAACTTAATTTTATAAACATAGAAAACTAGAATTGTTTTCAAATTAAAATTTAGTAGATATCCATGATTAATTTTTTAAAGAACAAAAAGAAAAATTCGTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAATCTGTCACAATTTTAGTTCTGTAAAGGAAATTTCGAAAACATATTTCTTGTTTACGAAATTTCAATAGGTTTTATAGAACACTTTTAAAAATTATTAAATACAACAACTACTAAAAAGAATAAGACATTCTAAGCCGAACTAATACTTAAATTTAAAAAAAACAACATTGTTATCAAACACAAACTTAAATTTCATTCCAAAGCCAGCATAGATTAGCGGTAATTGACAATTTACCTTCGACCAAAAGGTCAGAGGTTCAAATCCTACCACCCCAAATGTTGATATACTAAAAAAGCTTTAAAAATTTTCATTCTAACCTTTTTTTTTTTTTGAGTACATCAACATTCAGGGGTGGGGGATTTGAACCTCTGGCCTCATGGTCGAGGGTACATACCAATTACCGCTGAACTAAGCTCGCTTTGGCCATTTTAACCTTTTATATATACACATCTTTTTAAATTTTATTATCGATATTAATACTTTTGTCACCAGGTTAGTGATGAGTACAAGGTGTTCTGGCGACGGTGAAGACAATGAAGCAGAGAAGATAAGAACATTGGTAAAAGAGACGTTCGAAATAGCTACAAAAGTATCGTTTGGGAATGTGTTTGGATGGCCTTTAGAAAGGCTAGCCTTTTGGTTGTTTGGAAGACAAGCAAGGGATGTGACACTTAGGTATGATGAGATATTGGACAAAATATTAAGGCAACATGAAGAGAGAGCAAAGGAAGAGGGTTTGGATAGGGAAGATAGAGATTTAATGGACATTTTGTTGAAGGTTTATCAAGATGAGAATGCTGAATTCAAGATTACAAGAACCAATATCAAGGCTTTCTTGCTTGTAAGTCTTCTTTAATTATCATCTTATTTACAATTTTGCCTATTGAGAGACCTTTTAAACCTATTTCTCAAAACTCAACGATTAAAATGGTCTATTTTGAAAACTTTAGGGACCTTTTAAACCTACCTCTCAAAACTAAGAAATTCCGTTTTGAAAATTTAGGGATCAAATGCACCTATTCTCACAAATTCAGGGACCAAAAAGGTATTTTTCTCTATATTTTTGGAATATGACCTGAAGTTATTAACACACAAAATCGATAGTTGAAAGACCCATTTTTATACTTCAATCTATTAAACACAAAATTTGAATGTTCATAACTTAAACTTGAAACTTATTTGAGTTCATACACTGTGGATTTAAATTCTCCTTTTTTCTTAAGAATAATACTTAGCTACATATGTGTAGGTATCTCTCAATCCAATATCATTAAAATTTTGACAATTAGAAGAGATAACTTTTTTTCTTCTCAATTTCTATTTTTCTGATCTCTTAGCTGTCAAGATTTTAATGGTGGTGTTTAAGGAGCTACCTATATGCACTCGAGTATTGTTAACCATGAAGTTCCTTGTGATAGGATCTATTTGTGGGTGGAACCGGAACATCGACGGAGATAACCCAATGGGTGATGTCGGAGCTCCTCAACCATCCCAAAGCATTCGCCAAACTCAGGAACGAAATCAACTCTGTCGTCGGAACCACTAGACTCGTTGGAGAAGACGACATTCCAAATCTCCCTTACTTACAAGCTGTCGTCAAAGAGACTCTCCGACTCTACCCCCCGGTCCCTCTCGCCATGCGAGCCTGCCGTGAAGATTGCACAATCGATGGCTTCGACATCGCCAAAGGCACCATGGTTGCAGTCAACCTGTTCGCCATAATGCATGACCCTAAAACTTGGCAAAACCCCGACGAATTCGACCCCGAAAGGTTCATCGGCGGCGACGAGCGGTACGAGATAAAGGGGCAGCAGAGTTTCAAGTTTGTTCCCTTTGGTGGGGGCAGAAGGGCTTGCCCTGGCTCCACCTTGGCCTTCAGCTTCGTCAGTACAGTGGTTGCAGCCATGGTTCAATGCTTTGATTGGAAGATTGGGAAAAATGGAGATGAAACTCAAGTTAATATGGAGATTGCAGCTGCCTTCACTTTGCCTAGAGCCAACCCTCTTGACTGTGTTCCTGTTATTCGGTTAAACCCATTTGCTTTGGCAAACTGATGGCCTCCTATGCACTGAAAAAGTTAGTGCGTCGTATGCTCACTTTAGTTGTTTGTGTTTTGTGTATGATTTCAATTTGGTTTCTATGGTGATAAAAGTTTTAATTATGGTCGGTATGATTTGACCTAATCTTTGGAATGTGACGTCATTGTTGTTTTAATAAAAAGATA

mRNA sequence

GGAAGATTCCCACTACATTCTCTACATTGCTCTCTTCTTCGTCGTCTTCACCATTCTCCTTCAATATCTCCTTCACAGACCTTCAAAGAGCCTCACCCATGGCCGCCGCCGGCCGCCAAGTCCGCCGGCGCTGCCACTCATCGGCCACCTCCACTACTTCTCGCCGTACGTCAGCAAATCGTTCCACAATCTCGCCACCCGGTACGGCGACCTCCTCCTCCTCCGGCTAGGCAGCATCCAATGCGTCGTCGTTTCGTCGGCGGCGTACGCGGCGGAGGTCTACAAGAATCAGGACGTTATGTTCTCGTCGCGGCCGAAATTCGCGTTCGCCGACGAGCTGCCGTACGCGAAGGCCGGATTCTTCGGGGCGGAGTACGGGAGTTACTGGAGATATATGAAGAAACTGACGATGACGGAGCTTTTATCGCAGCGGCAAGTTGAGCGATCACGCGGCGTACGGAGAGAAGAAATGTTGAAATTCTTGAGGAAATTGGCGGATTCCGGCGAGAAGAACGAGGCTCTGGATTTGGGCGCCGAGCTCATCAAACTCACCAACAATAGTATTTGTAGGTTAGTGATGAGTACAAGGTGTTCTGGCGACGGTGAAGACAATGAAGCAGAGAAGATAAGAACATTGGTAAAAGAGACGTTCGAAATAGCTACAAAAGTATCGTTTGGGAATGTGTTTGGATGGCCTTTAGAAAGGCTAGCCTTTTGGTTGTTTGGAAGACAAGCAAGGGATGTGACACTTAGGTATGATGAGATATTGGACAAAATATTAAGGCAACATGAAGAGAGAGCAAAGGAAGAGGGTTTGGATAGGGAAGATAGAGATTTAATGGACATTTTGTTGAAGGTTTATCAAGATGAGAATGCTGAATTCAAGATTACAAGAACCAATATCAAGGCTTTCTTGCTTGATCTATTTGTGGGTGGAACCGGAACATCGACGGAGATAACCCAATGGGTGATGTCGGAGCTCCTCAACCATCCCAAAGCATTCGCCAAACTCAGGAACGAAATCAACTCTGTCGTCGGAACCACTAGACTCGTTGGAGAAGACGACATTCCAAATCTCCCTTACTTACAAGCTGTCGTCAAAGAGACTCTCCGACTCTACCCCCCGGTCCCTCTCGCCATGCGAGCCTGCCGTGAAGATTGCACAATCGATGGCTTCGACATCGCCAAAGGCACCATGGTTGCAGTCAACCTGTTCGCCATAATGCATGACCCTAAAACTTGGCAAAACCCCGACGAATTCGACCCCGAAAGGTTCATCGGCGGCGACGAGCGGTACGAGATAAAGGGGCAGCAGAGTTTCAAGTTTGTTCCCTTTGGTGGGGGCAGAAGGGCTTGCCCTGGCTCCACCTTGGCCTTCAGCTTCGTCAGTACAGTGGTTGCAGCCATGGTTCAATGCTTTGATTGGAAGATTGGGAAAAATGGAGATGAAACTCAAGTTAATATGGAGATTGCAGCTGCCTTCACTTTGCCTAGAGCCAACCCTCTTGACTGTGTTCCTGTTATTCGGTTAAACCCATTTGCTTTGGCAAACTGATGGCCTCCTATGCACTGAAAAAGTTAGTGCGTCGTATGCTCACTTTAGTTGTTTGTGTTTTGTGTATGATTTCAATTTGGTTTCTATGGTGATAAAAGTTTTAATTATGGTCGGTATGATTTGACCTAATCTTTGGAATGTGACGTCATTGTTGTTTTAATAAAAAGATA

Coding sequence (CDS)

ATGTTCTCGTCGCGGCCGAAATTCGCGTTCGCCGACGAGCTGCCGTACGCGAAGGCCGGATTCTTCGGGGCGGAGTACGGGAGTTACTGGAGATATATGAAGAAACTGACGATGACGGAGCTTTTATCGCAGCGGCAAGTTGAGCGATCACGCGGCGTACGGAGAGAAGAAATGTTGAAATTCTTGAGGAAATTGGCGGATTCCGGCGAGAAGAACGAGGCTCTGGATTTGGGCGCCGAGCTCATCAAACTCACCAACAATAGTATTTGTAGGTTAGTGATGAGTACAAGGTGTTCTGGCGACGGTGAAGACAATGAAGCAGAGAAGATAAGAACATTGGTAAAAGAGACGTTCGAAATAGCTACAAAAGTATCGTTTGGGAATGTGTTTGGATGGCCTTTAGAAAGGCTAGCCTTTTGGTTGTTTGGAAGACAAGCAAGGGATGTGACACTTAGGTATGATGAGATATTGGACAAAATATTAAGGCAACATGAAGAGAGAGCAAAGGAAGAGGGTTTGGATAGGGAAGATAGAGATTTAATGGACATTTTGTTGAAGGTTTATCAAGATGAGAATGCTGAATTCAAGATTACAAGAACCAATATCAAGGCTTTCTTGCTTGATCTATTTGTGGGTGGAACCGGAACATCGACGGAGATAACCCAATGGGTGATGTCGGAGCTCCTCAACCATCCCAAAGCATTCGCCAAACTCAGGAACGAAATCAACTCTGTCGTCGGAACCACTAGACTCGTTGGAGAAGACGACATTCCAAATCTCCCTTACTTACAAGCTGTCGTCAAAGAGACTCTCCGACTCTACCCCCCGGTCCCTCTCGCCATGCGAGCCTGCCGTGAAGATTGCACAATCGATGGCTTCGACATCGCCAAAGGCACCATGGTTGCAGTCAACCTGTTCGCCATAATGCATGACCCTAAAACTTGGCAAAACCCCGACGAATTCGACCCCGAAAGGTTCATCGGCGGCGACGAGCGGTACGAGATAAAGGGGCAGCAGAGTTTCAAGTTTGTTCCCTTTGGTGGGGGCAGAAGGGCTTGCCCTGGCTCCACCTTGGCCTTCAGCTTCGTCAGTACAGTGGTTGCAGCCATGGTTCAATGCTTTGATTGGAAGATTGGGAAAAATGGAGATGAAACTCAAGTTAATATGGAGATTGCAGCTGCCTTCACTTTGCCTAGAGCCAACCCTCTTGACTGTGTTCCTGTTATTCGGTTAAACCCATTTGCTTTGGCAAACTGA

Protein sequence

MFSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFLRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIATKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMDILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEINSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVAVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSFVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALAN
Homology
BLAST of Lcy02g004290 vs. ExPASy Swiss-Prot
Match: Q9LJY7 (Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.3e-94
Identity = 182/412 (44.17%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 3   SSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFL 62
           SSR      + L    + F GA YG YW++MKKL +T+LL  + +ERSR +R +E+ +F 
Sbjct: 105 SSRDNPPINESLLVGSSVFVGAPYGDYWKFMKKLLVTKLLGPQALERSRSIRADELERFY 164

Query: 63  RKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIAT 122
           R L D   K E++++G E  KL+ NSICR+ M    S   E  EAE++R LV E   +  
Sbjct: 165 RSLLDKAMKKESVEIGKEATKLSINSICRMSMGR--SFSEESGEAERVRGLVTELDGLTK 224

Query: 123 KVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMD 182
           KV   N+  WPLE+L   LF ++   V+  +DE+L++I+ + E++  E     +   LMD
Sbjct: 225 KVLLVNILRWPLEKLRISLFKKEIMYVSNSFDELLERIIVEREKKPNE----HQGTYLMD 284

Query: 183 ILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEI 242
           +LL+ Y+DE AE KITR +IK+  ++L +GGT TS +  QW M+EL+N+     +LR EI
Sbjct: 285 VLLEAYEDEKAEHKITRNHIKSLFVELLLGGTDTSAQTIQWTMAELINNRNVLKRLREEI 344

Query: 243 NSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVA 302
           +SVVG TRL+ E D+P LPYLQ+VVKE LRL+PP+PL +R  +  C + GF IA+ T + 
Sbjct: 345 DSVVGETRLIQEKDLPKLPYLQSVVKEGLRLHPPLPLMVRTFQRSCEMKGFYIAEKTTLV 404

Query: 303 VNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSF 362
           VN +A+M DP TW++PDEF PERF+  +E      +++ K + FG GRR CPGS LA  F
Sbjct: 405 VNAYAVMRDPTTWEDPDEFKPERFLRQEEE-----RRALKHIAFGSGRRGCPGSNLATIF 464

Query: 363 VSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           + T +  MVQCFD  I   GD  +V M+      L  A+PL+C+ V R  PF
Sbjct: 465 IGTAIGTMVQCFDLSI--KGD--KVKMDEVGGLNLTMAHPLECILVPRTQPF 501

BLAST of Lcy02g004290 vs. ExPASy Swiss-Prot
Match: Q9FI39 (Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.9e-93
Identity = 180/410 (43.90%), Postives = 261/410 (63.66%), Query Frame = 0

Query: 3   SSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFL 62
           SSRP     + L    + F    YG Y ++MKK  + +LL  + ++RSR +R +E+ +F 
Sbjct: 108 SSRPPPPIEESLILGSSSFINTPYGDYSKFMKKFMVQKLLGPQALQRSRNIRADELERFY 167

Query: 63  RKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIAT 122
           + L D   K + +++  E +KLTNN+IC+++M   CS   E+ EAE +R LV E+  +  
Sbjct: 168 KTLLDKAMKKQTVEIRNEAMKLTNNTICKMIMGRSCS--EENGEAETVRGLVTESIFLTK 227

Query: 123 KVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMD 182
           K   G +F  PL++L   LF ++  +V+ R+DE+L+KIL +HEE+ +E     +  D++D
Sbjct: 228 KHFLGAMFHKPLKKLGISLFAKELMNVSNRFDELLEKILVEHEEKLQEH---HQTSDMLD 287

Query: 183 ILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEI 242
           +LL+ Y DENAE+KITR  IK+  +DLF  GT  S    QW M+E++ +PK   +LR EI
Sbjct: 288 MLLEAYGDENAEYKITRDQIKSLFVDLFSAGTEASANTIQWTMAEIIKNPKICERLREEI 347

Query: 243 NSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVA 302
           +SVVG TRLV E D+PNLPYLQA+VKE LRL+PP P+ +R  +E C I GF I + T + 
Sbjct: 348 DSVVGKTRLVQETDLPNLPYLQAIVKEGLRLHPPGPV-VRTFKETCEIKGFYIPEKTRLF 407

Query: 303 VNLFAIMHDPKTWQNPDEFDPERFIGGDE--RYEIKGQQSFKFVPFGGGRRACPGSTLAF 362
           VN++AIM DP  W++P+EF PERF+        + K +   K++PFG GRRACPGS LA+
Sbjct: 408 VNVYAIMRDPDFWEDPEEFKPERFLASSRLGEEDEKREDMLKYIPFGSGRRACPGSHLAY 467

Query: 363 SFVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIR 411
           + V +V+  MVQ FDW I   G+  ++NM+     TL  A+PL C PV R
Sbjct: 468 TVVGSVIGMMVQHFDWII--KGE--KINMKEGGTMTLTMAHPLKCTPVPR 507

BLAST of Lcy02g004290 vs. ExPASy Swiss-Prot
Match: Q9FH67 (Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.6e-91
Identity = 175/408 (42.89%), Postives = 255/408 (62.50%), Query Frame = 0

Query: 12  DELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFLRKLADSGEK 71
           D L +  +GF  A YG YW++MKKL  T+LL    +E S+G R EE+ +F   L     K
Sbjct: 114 DSLVFGSSGFVTAPYGDYWKFMKKLISTKLLRPHALELSKGNRAEELRRFCLDLQGKARK 173

Query: 72  NEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIATKVSFGNVFG 131
            E++++G   +KLTNN ICR+ M   CS   ++  AE+ R LV ++F ++ K+ F N   
Sbjct: 174 KESVEIGKVALKLTNNIICRMSMGRSCS--EKNGVAERARELVNKSFALSVKLFFSN--- 233

Query: 132 WPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMDILLKVYQDE 191
                    +F +    V+  +DE L++IL +HEE  + +    +DRD++D LL+ Y++E
Sbjct: 234 ---------MFRKDIMGVSREFDEFLERILVEHEENLEGD----QDRDMIDHLLEAYRNE 293

Query: 192 NAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEINSVVGTTRL 251
            AE+KITR  IK+ ++++F+GGT +S +  QW M+E+LN+P    KLR EI+SVVG  RL
Sbjct: 294 EAEYKITRKQIKSLIVEIFLGGTDSSAQTIQWTMAEILNNPGVLEKLRAEIDSVVGGKRL 353

Query: 252 VGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVAVNLFAIMHD 311
           + E D+PNLPYLQAVVKE LRL+P  P+ +R   E C +  F + + T + VNL+A+  D
Sbjct: 354 IQESDLPNLPYLQAVVKEGLRLHPSAPVLLRVFGESCEVKEFYVPEKTTLVVNLYAVNRD 413

Query: 312 PKTWQNPDEFDPERF----IGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSFVSTVV 371
           P +W++PD F PERF    I GDE  E   +Q+ K+V FGGGRR CP   LA  F+ T +
Sbjct: 414 PDSWEDPDMFKPERFLVSSISGDE--EKIREQAVKYVTFGGGRRTCPAVKLAHIFMETAI 473

Query: 372 AAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFA 416
            AMVQCFDW+I   G++  +  E  +  +L  A+PL C PV+R +PF+
Sbjct: 474 GAMVQCFDWRI--KGEKVYME-EAVSGLSLKMAHPLKCTPVVRFDPFS 498

BLAST of Lcy02g004290 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.5e-90
Identity = 173/399 (43.36%), Postives = 263/399 (65.91%), Query Frame = 0

Query: 21  FFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFLRKLADSGEKNEALDLGAE 80
           F  A +G YW++MKKL M+ELLS R +++   VR++E  +F+ ++   G   EA+D G E
Sbjct: 115 FAFAPFGPYWKFMKKLCMSELLSGRMMDQFLPVRQQETKRFISRVFRKGVAGEAVDFGDE 174

Query: 81  LIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIATKVSFGNVFGWPLERLAFW 140
           L+ L+NN + R+ +S + S +  DN+AE+++ LV    E+  K +  + F W L+     
Sbjct: 175 LMTLSNNIVSRMTLSQKTSEN--DNQAEEMKKLVSNIAELMGKFNVSD-FIWYLKPFDLQ 234

Query: 141 LFGRQARDVTLRYDEILDKILRQHEE---RAKEEGLDREDRDLMDILLKVYQDENAEFKI 200
            F R+ ++   R+D ++D I++Q +E   + KE G  ++ +D++D+LL +++DENAE K+
Sbjct: 235 GFNRKIKETRDRFDVVVDGIIKQRQEERRKNKETGTAKQFKDMLDVLLDMHEDENAEIKL 294

Query: 201 TRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEINSVVGTTRLVGEDDI 260
            + NIKAF++D+FV GT TS    +W M+EL+N+P    K R EI++VVG +R+V E DI
Sbjct: 295 DKKNIKAFIMDIFVAGTDTSAVSIEWAMAELINNPDVLEKARQEIDAVVGKSRMVEESDI 354

Query: 261 PNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVAVNLFAIMHDPKTWQN 320
            NLPYLQA+V+ETLRL+P  PL +R   +   + G+DI   T + VN++AI  DP  W+ 
Sbjct: 355 ANLPYLQAIVRETLRLHPGGPLVVRESSKSAVVCGYDIPAKTRLFVNVWAIGRDPNHWEK 414

Query: 321 PDEFDPERFI-GGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSFVSTVVAAMVQCFDW 380
           P EF PERFI  G  + +++GQ  + F+PFG GRR CPG++LA+  V   +A ++QCF W
Sbjct: 415 PFEFRPERFIRDGQNQLDVRGQH-YHFIPFGSGRRTCPGASLAWQVVPVNLAIIIQCFQW 474

Query: 381 K-IGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           K +G NG   +V+ME  +  TLPRANP+ CVPV R+NPF
Sbjct: 475 KLVGGNG---KVDMEEKSGITLPRANPIICVPVPRINPF 506

BLAST of Lcy02g004290 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 7.3e-90
Identity = 176/418 (42.11%), Postives = 264/418 (63.16%), Query Frame = 0

Query: 2   FSSRPKFAFADE-LPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLK 61
           FS+RP    A E L Y    F  A YG YW++MKKL M+ELL    +++   VR+ E  K
Sbjct: 95  FSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPVRQXETKK 154

Query: 62  FLRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEI 121
           F++++   G   EA+D G E I L+NN + R+++S + S   ++NE E++R LVK+  E+
Sbjct: 155 FIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVS-QTSTTEDENEVEEMRKLVKDAAEL 214

Query: 122 ATKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEE--RAKEEGL-DRED 181
           + K +  +   + L+R     F ++   +   +D +LD+I++Q EE  R K E +  RE 
Sbjct: 215 SGKFNISDFVSF-LKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETVGKREF 274

Query: 182 RDLMDILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAK 241
           +D++D+L  + +DE++E K+ + NIKAF+LD+ + GT TS    +W M+EL+N+P    K
Sbjct: 275 KDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNPGVLEK 334

Query: 242 LRNEINSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAK 301
            R E+++VVG +R+V E DI NLPYLQ +V+ETLRL+P  PL  R       + G+DI  
Sbjct: 335 ARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRESSRRAVVCGYDIPA 394

Query: 302 GTMVAVNLFAIMHDPKTWQNPDEFDPERFI-GGDERYEIKGQQSFKFVPFGGGRRACPGS 361
            T + VN++AI  DP  W+NP EF PERF+  G  + +++GQ  +  +PFG GRRACPG+
Sbjct: 395 KTRLFVNVWAIGRDPNHWENPLEFRPERFVENGKSQLDVRGQH-YHLLPFGSGRRACPGT 454

Query: 362 TLAFSFVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           +LA   V   +A ++QCF WK+  + D  +VNME  A  TLPRA+P+ CVP+ RLNPF
Sbjct: 455 SLALQVVHVNLAVLIQCFQWKV--DCDNGKVNMEEKAGITLPRAHPIICVPIRRLNPF 507

BLAST of Lcy02g004290 vs. ExPASy TrEMBL
Match: A0A6J1DFG8 (cytochrome P450 705A5-like OS=Momordica charantia OX=3673 GN=LOC111020330 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.5e-199
Identity = 342/417 (82.01%), Postives = 380/417 (91.13%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FS RP FAF DEL YAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSR VRREEM+KF
Sbjct: 102 FSWRPNFAFGDELLYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMVKF 161

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           LRKLA+SG KNEA+DLGAEL+KLTNNS CR+VMSTRCSGD +D+EAEKIR LVKETF++A
Sbjct: 162 LRKLAESGHKNEAVDLGAELVKLTNNSTCRMVMSTRCSGD-DDDEAEKIRVLVKETFQMA 221

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           T+V+FG+VFGWPLERLAFWLFGRQA+DVTLRYDEIL+KIL+QHEERAKEEGLD EDRDLM
Sbjct: 222 TQVAFGDVFGWPLERLAFWLFGRQAQDVTLRYDEILEKILKQHEERAKEEGLDGEDRDLM 281

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEFKITRTNIKAFLLDLF+GGTGTSTE++QW M+ELLNHPK F KLR+E
Sbjct: 282 DILLKVYQDHNAEFKITRTNIKAFLLDLFLGGTGTSTEVSQWAMAELLNHPKVFTKLRDE 341

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+S++GT RLVGEDD+PNLPYLQAVVKE LRLYP VPL+MR+CREDCTIDG+ + K TMV
Sbjct: 342 IDSIIGTDRLVGEDDLPNLPYLQAVVKEALRLYPAVPLSMRSCREDCTIDGYVVPKNTMV 401

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+NP+EFDPERF  GD +YEIKGQQSFKFVPFGGGRRACPGS LAFS
Sbjct: 402 AVNLFDIMRDPKAWENPNEFDPERF-NGDMKYEIKGQQSFKFVPFGGGRRACPGSNLAFS 461

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALAN 419
           F+S V+AAMVQCFDWKIG+NGDE +VNMEI +AFTLP ANPL CVPV+R N FA AN
Sbjct: 462 FISNVIAAMVQCFDWKIGRNGDENKVNMEIGSAFTLPMANPLLCVPVVRFNFFASAN 516

BLAST of Lcy02g004290 vs. ExPASy TrEMBL
Match: A0A6J1GBA3 (cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452598 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.8e-195
Identity = 334/416 (80.29%), Postives = 379/416 (91.11%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYA AGFFGAEYG YWR+MKKLTMTELLSQRQVERSR VRR+EM+KF
Sbjct: 101 FSSRPKFAFGDELPYANAGFFGAEYGGYWRFMKKLTMTELLSQRQVERSRCVRRDEMVKF 160

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           L KLA+ GE+N+A+DLGAELI+L NNSICRL+MSTRC G+  D+EAE IRTL++ET E+A
Sbjct: 161 LVKLAECGERNDAVDLGAELIRLANNSICRLLMSTRCCGN--DDEAETIRTLIRETMEVA 220

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           TKVSFGNVFG PLE+LAFWLFGRQ RD+TLRYDEIL+K+L+QHE RAKE+   REDRDLM
Sbjct: 221 TKVSFGNVFGGPLEKLAFWLFGRQVRDLTLRYDEILEKVLKQHEHRAKEDDFGREDRDLM 280

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILL+VY+DEN+EFKITRT+IKAF LDL +GGTGTSTE+ QWVMSELLNHPK F KLRNE
Sbjct: 281 DILLRVYRDENSEFKITRTHIKAFFLDLLLGGTGTSTEVAQWVMSELLNHPKVFNKLRNE 340

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+SV+GTT+LVGEDD+PNLPYLQAVVKETLRLYP VP+AMRACR+DC I+GFD+ K  MV
Sbjct: 341 IDSVIGTTKLVGEDDVPNLPYLQAVVKETLRLYPAVPIAMRACRQDCKINGFDVPKDMMV 400

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLFAIM DPK W+NP+EFDPERF  G+ERYEIKGQQSFKFVPFGGGRRACPGSTLAF 
Sbjct: 401 AVNLFAIMRDPKVWENPNEFDPERFT-GEERYEIKGQQSFKFVPFGGGRRACPGSTLAFG 460

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALA 418
           F+STVVAAMVQCFDWKIG+NGDE++V+MEI AAFTLPRANPL CVPV+R +P +LA
Sbjct: 461 FISTVVAAMVQCFDWKIGRNGDESKVDMEIGAAFTLPRANPLLCVPVLRFDPSSLA 513

BLAST of Lcy02g004290 vs. ExPASy TrEMBL
Match: A0A5A7U3V6 (Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00360 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.8e-193
Identity = 334/418 (79.90%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSRGVRREEMLK 
Sbjct: 98  FSSRPKFAFGDELPYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKL 157

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           LRKL + GEK E +DLGAEL+KLTNNS CRLVMSTRCSGD +D+EAEKIR LVKETFE+A
Sbjct: 158 LRKLCECGEKKEVVDLGAELVKLTNNSTCRLVMSTRCSGD-DDDEAEKIRLLVKETFEMA 217

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           +KV+FG+VFGWPLERLAFW+FGRQA+DVTLRYDEIL+ ILRQHE+R +EEGLDREDRDLM
Sbjct: 218 SKVAFGDVFGWPLERLAFWMFGRQAKDVTLRYDEILENILRQHEDRGREEGLDREDRDLM 277

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEF ITRTNIKAFLLDLF+GGTGTSTE++QW M+ELLNHPK F KLRNE
Sbjct: 278 DILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGTSTEVSQWTMAELLNHPKVFNKLRNE 337

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           INS+VGT+RLVGEDD+PNLPYLQAVVKE LRLYP VP+AMR CR+DCTIDG+ I K TMV
Sbjct: 338 INSIVGTSRLVGEDDLPNLPYLQAVVKEALRLYPAVPIAMRTCRQDCTIDGYHIPKDTMV 397

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+N +EFDPERF  GD +YE KGQQSF FVPFGGGRRACPGSTLAFS
Sbjct: 398 AVNLFDIMRDPKIWKNSNEFDPERFT-GDVQYEFKGQQSFNFVPFGGGRRACPGSTLAFS 457

Query: 362 FVSTVVAAMVQCFDWKIGKNGDE-----TQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           F+S V+A MVQCFDWKI    D      ++V+MEI AAFTLP ANPL CVP++R NPF
Sbjct: 458 FISNVIATMVQCFDWKIIGRSDNNRDGISKVDMEIGAAFTLPMANPLRCVPIVRFNPF 513

BLAST of Lcy02g004290 vs. ExPASy TrEMBL
Match: A0A6J1K7Z6 (cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493062 PE=3 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.4e-192
Identity = 331/416 (79.57%), Postives = 378/416 (90.87%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYA AGFFGAEYG YWR+MKKLTMTELLSQRQVERSR VRR+EM+KF
Sbjct: 101 FSSRPKFAFGDELPYATAGFFGAEYGGYWRFMKKLTMTELLSQRQVERSRAVRRDEMVKF 160

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           L KLA+ GE+N+A+D+GAELI+L NNSICRL+MSTRC G+  D+EAEKI+ LVKET EIA
Sbjct: 161 LVKLAECGERNDAVDMGAELIRLANNSICRLLMSTRCCGN--DDEAEKIKKLVKETMEIA 220

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           TKVSFGNVFG P+E+LAFWLFGRQ RD+TLRYDEIL+K+L+QHE RAKE+G DREDRDLM
Sbjct: 221 TKVSFGNVFGGPMEKLAFWLFGRQVRDLTLRYDEILEKVLKQHEHRAKEDGFDREDRDLM 280

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILL+VY+DENAEFKITRT+IKAF LDL +GGTGTSTE+ QWVMSELLNHP+ F KLRNE
Sbjct: 281 DILLRVYRDENAEFKITRTHIKAFFLDLLLGGTGTSTEVAQWVMSELLNHPEVFNKLRNE 340

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+ VVGTT+LVGEDD+PNLPYLQAVVKETLRLYP VP+AMRACR+DC I+GFD+ K TMV
Sbjct: 341 IDFVVGTTKLVGEDDVPNLPYLQAVVKETLRLYPAVPIAMRACRQDCKINGFDVPKDTMV 400

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF++M DPK W+NP+EF+PERF  G+ERYEIKG Q FKFVPFGGGRRACPGSTLAF 
Sbjct: 401 AVNLFSVMRDPKVWKNPNEFNPERFT-GEERYEIKG-QIFKFVPFGGGRRACPGSTLAFG 460

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALA 418
           F+STVVAAMVQCFDW IG+NGDE++V+MEI AAFTLPRANPL CVPV+R +P +LA
Sbjct: 461 FISTVVAAMVQCFDWTIGRNGDESKVDMEIGAAFTLPRANPLLCVPVLRFDPCSLA 512

BLAST of Lcy02g004290 vs. ExPASy TrEMBL
Match: A0A6J1IYT7 (cytochrome P450 705A5-like OS=Cucurbita maxima OX=3661 GN=LOC111481896 PE=3 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.3e-192
Identity = 336/409 (82.15%), Postives = 367/409 (89.73%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSR VRREEMLKF
Sbjct: 97  FSSRPKFAFGDELPYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMLKF 156

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           L+KLAD GEKNEA+DLGAEL+KLTNNS CRLVMSTRCSGD  D EAEKIR LVKETFE+A
Sbjct: 157 LKKLADCGEKNEAVDLGAELVKLTNNSTCRLVMSTRCSGDDSD-EAEKIRVLVKETFEMA 216

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           TKV+FG+VFGWPLERLAFW+FG+ A+DVT+RYDEIL+KILRQHEERAKEEGLDREDRDLM
Sbjct: 217 TKVAFGDVFGWPLERLAFWMFGKHAKDVTMRYDEILEKILRQHEERAKEEGLDREDRDLM 276

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEFKITRTNIKAF LDLF+GGTGTSTE++QW M+ELLNHP+ F KLRNE
Sbjct: 277 DILLKVYQDHNAEFKITRTNIKAFFLDLFLGGTGTSTEVSQWTMAELLNHPEVFNKLRNE 336

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           IN+VVGTTRLVGEDD+PNLPYLQAVVKE LRLYP VP+AMRA REDCTIDGF + K TMV
Sbjct: 337 INAVVGTTRLVGEDDVPNLPYLQAVVKEALRLYPAVPIAMRASREDCTIDGFHVPKDTMV 396

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+NP+EF+PERF  G+ RYEIKGQQ   FVPFGGGRR CPGSTLAFS
Sbjct: 397 AVNLFHIMRDPKIWENPNEFEPERFT-GEVRYEIKGQQGSGFVPFGGGRRGCPGSTLAFS 456

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIR 411
           F+STV AAMVQCFDWKI      ++V+MEI AAFTLP ANPL CVP++R
Sbjct: 457 FISTVTAAMVQCFDWKIIGG---SKVDMEIGAAFTLPMANPLLCVPLLR 500

BLAST of Lcy02g004290 vs. NCBI nr
Match: XP_022152668.1 (cytochrome P450 705A5-like [Momordica charantia])

HSP 1 Score: 705.3 bits (1819), Expect = 3.2e-199
Identity = 342/417 (82.01%), Postives = 380/417 (91.13%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FS RP FAF DEL YAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSR VRREEM+KF
Sbjct: 102 FSWRPNFAFGDELLYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMVKF 161

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           LRKLA+SG KNEA+DLGAEL+KLTNNS CR+VMSTRCSGD +D+EAEKIR LVKETF++A
Sbjct: 162 LRKLAESGHKNEAVDLGAELVKLTNNSTCRMVMSTRCSGD-DDDEAEKIRVLVKETFQMA 221

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           T+V+FG+VFGWPLERLAFWLFGRQA+DVTLRYDEIL+KIL+QHEERAKEEGLD EDRDLM
Sbjct: 222 TQVAFGDVFGWPLERLAFWLFGRQAQDVTLRYDEILEKILKQHEERAKEEGLDGEDRDLM 281

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEFKITRTNIKAFLLDLF+GGTGTSTE++QW M+ELLNHPK F KLR+E
Sbjct: 282 DILLKVYQDHNAEFKITRTNIKAFLLDLFLGGTGTSTEVSQWAMAELLNHPKVFTKLRDE 341

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+S++GT RLVGEDD+PNLPYLQAVVKE LRLYP VPL+MR+CREDCTIDG+ + K TMV
Sbjct: 342 IDSIIGTDRLVGEDDLPNLPYLQAVVKEALRLYPAVPLSMRSCREDCTIDGYVVPKNTMV 401

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+NP+EFDPERF  GD +YEIKGQQSFKFVPFGGGRRACPGS LAFS
Sbjct: 402 AVNLFDIMRDPKAWENPNEFDPERF-NGDMKYEIKGQQSFKFVPFGGGRRACPGSNLAFS 461

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALAN 419
           F+S V+AAMVQCFDWKIG+NGDE +VNMEI +AFTLP ANPL CVPV+R N FA AN
Sbjct: 462 FISNVIAAMVQCFDWKIGRNGDENKVNMEIGSAFTLPMANPLLCVPVVRFNFFASAN 516

BLAST of Lcy02g004290 vs. NCBI nr
Match: XP_023525669.1 (cytochrome P450 705A22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.1 bits (1803), Expect = 2.3e-197
Identity = 337/416 (81.01%), Postives = 381/416 (91.59%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYA AGFFGAEYG YWR+MKKLTMTELLSQRQVERSRGVRR+EM+KF
Sbjct: 101 FSSRPKFAFGDELPYANAGFFGAEYGGYWRFMKKLTMTELLSQRQVERSRGVRRDEMVKF 160

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           L KLA+ GE+N+A+DLGAELI+L NNSICRL+MSTRC G+  D+EAE IRTLV+ET E+A
Sbjct: 161 LVKLAECGERNDAVDLGAELIRLANNSICRLLMSTRCCGN--DDEAETIRTLVRETMEVA 220

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           TKVSFGNVFG PLE+LAFWLFGRQ RD+TLRYDEIL+K+L+QHE RAKE+  DREDRDLM
Sbjct: 221 TKVSFGNVFGGPLEKLAFWLFGRQVRDLTLRYDEILEKVLKQHEHRAKEDDFDREDRDLM 280

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILL+VY+DEN+EFKITRT+IKAF LDL +GGTGTSTE+ QWVMSELLNHPK F KLRNE
Sbjct: 281 DILLRVYRDENSEFKITRTHIKAFFLDLLLGGTGTSTEVAQWVMSELLNHPKVFNKLRNE 340

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+SV+GTT+LVGEDD+PNLPYLQAVVKETLRLYP VP+AMRACR+DC I+GFD+ K TMV
Sbjct: 341 IDSVIGTTKLVGEDDVPNLPYLQAVVKETLRLYPAVPIAMRACRQDCKINGFDVPKDTMV 400

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+NP+EFDPERF  G+ERYEIKGQQSFKFVPFGGGRRACPGSTLAF 
Sbjct: 401 AVNLFTIMRDPKVWENPNEFDPERFT-GEERYEIKGQQSFKFVPFGGGRRACPGSTLAFG 460

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALA 418
           F+STVVAAMVQCFDWKIG+NGDE++V+MEI AAFTLPRANPL CVPV+R +P +LA
Sbjct: 461 FISTVVAAMVQCFDWKIGRNGDESKVDMEIGAAFTLPRANPLLCVPVLRFDPSSLA 513

BLAST of Lcy02g004290 vs. NCBI nr
Match: XP_038905910.1 (cytochrome P450 705A5-like [Benincasa hispida])

HSP 1 Score: 696.0 bits (1795), Expect = 1.9e-196
Identity = 344/416 (82.69%), Postives = 374/416 (89.90%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSRGVRREEMLK 
Sbjct: 97  FSSRPKFAFGDELPYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKL 156

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           LRKL + GEK EA+DLGAEL+KLTNNS CRLVMSTRCSGD +D+EAEKIR LVKETFE+A
Sbjct: 157 LRKLWECGEKKEAVDLGAELVKLTNNSTCRLVMSTRCSGD-DDDEAEKIRLLVKETFEMA 216

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           +KV+FG+VFGWPLERLAFW+FGR A+DVT+RYDEIL+KILRQHEERA+EEGLDRED DLM
Sbjct: 217 SKVAFGDVFGWPLERLAFWMFGRHAKDVTMRYDEILEKILRQHEERAREEGLDREDTDLM 276

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEF ITRTNIKAFLLDLF+GGTGTSTE++QW M+ELLNHPK   KLRNE
Sbjct: 277 DILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGTSTEVSQWAMAELLNHPKVLNKLRNE 336

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           INSVVGTTR+VGEDD+PNLPYLQAVVKE LRLYP VP+AMRACR+DC IDGFDI K TMV
Sbjct: 337 INSVVGTTRVVGEDDLPNLPYLQAVVKEALRLYPAVPIAMRACRQDCKIDGFDIPKDTMV 396

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK WQNP+EFDPERF  GD RYEIKGQQSF FVPFGGGRRACPGSTLAFS
Sbjct: 397 AVNLFDIMRDPKIWQNPNEFDPERFT-GDVRYEIKGQQSFDFVPFGGGRRACPGSTLAFS 456

Query: 362 FVSTVVAAMVQCFDWKI---GKNGD-ETQVNMEIAAAFTLPRANPLDCVPVIRLNP 414
           F+S V+AAMVQCFDWK+     NGD  ++VNMEI AAFTLP ANPL CVPV+R NP
Sbjct: 457 FISNVIAAMVQCFDWKVIGRDDNGDGRSKVNMEIGAAFTLPMANPLLCVPVVRFNP 510

BLAST of Lcy02g004290 vs. NCBI nr
Match: XP_022949167.1 (cytochrome P450 705A22-like [Cucurbita moschata])

HSP 1 Score: 691.8 bits (1784), Expect = 3.6e-195
Identity = 334/416 (80.29%), Postives = 379/416 (91.11%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYA AGFFGAEYG YWR+MKKLTMTELLSQRQVERSR VRR+EM+KF
Sbjct: 101 FSSRPKFAFGDELPYANAGFFGAEYGGYWRFMKKLTMTELLSQRQVERSRCVRRDEMVKF 160

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           L KLA+ GE+N+A+DLGAELI+L NNSICRL+MSTRC G+  D+EAE IRTL++ET E+A
Sbjct: 161 LVKLAECGERNDAVDLGAELIRLANNSICRLLMSTRCCGN--DDEAETIRTLIRETMEVA 220

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           TKVSFGNVFG PLE+LAFWLFGRQ RD+TLRYDEIL+K+L+QHE RAKE+   REDRDLM
Sbjct: 221 TKVSFGNVFGGPLEKLAFWLFGRQVRDLTLRYDEILEKVLKQHEHRAKEDDFGREDRDLM 280

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILL+VY+DEN+EFKITRT+IKAF LDL +GGTGTSTE+ QWVMSELLNHPK F KLRNE
Sbjct: 281 DILLRVYRDENSEFKITRTHIKAFFLDLLLGGTGTSTEVAQWVMSELLNHPKVFNKLRNE 340

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           I+SV+GTT+LVGEDD+PNLPYLQAVVKETLRLYP VP+AMRACR+DC I+GFD+ K  MV
Sbjct: 341 IDSVIGTTKLVGEDDVPNLPYLQAVVKETLRLYPAVPIAMRACRQDCKINGFDVPKDMMV 400

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLFAIM DPK W+NP+EFDPERF  G+ERYEIKGQQSFKFVPFGGGRRACPGSTLAF 
Sbjct: 401 AVNLFAIMRDPKVWENPNEFDPERFT-GEERYEIKGQQSFKFVPFGGGRRACPGSTLAFG 460

Query: 362 FVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFALA 418
           F+STVVAAMVQCFDWKIG+NGDE++V+MEI AAFTLPRANPL CVPV+R +P +LA
Sbjct: 461 FISTVVAAMVQCFDWKIGRNGDESKVDMEIGAAFTLPRANPLLCVPVLRFDPSSLA 513

BLAST of Lcy02g004290 vs. NCBI nr
Match: KAA0048325.1 (cytochrome P450 705A5-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 684.5 bits (1765), Expect = 5.8e-193
Identity = 334/418 (79.90%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRPKFAF DELPYAKAGFF AEYG YWR+MKKLTMTELLSQRQVERSRGVRREEMLK 
Sbjct: 98  FSSRPKFAFGDELPYAKAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKL 157

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           LRKL + GEK E +DLGAEL+KLTNNS CRLVMSTRCSGD +D+EAEKIR LVKETFE+A
Sbjct: 158 LRKLCECGEKKEVVDLGAELVKLTNNSTCRLVMSTRCSGD-DDDEAEKIRLLVKETFEMA 217

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
           +KV+FG+VFGWPLERLAFW+FGRQA+DVTLRYDEIL+ ILRQHE+R +EEGLDREDRDLM
Sbjct: 218 SKVAFGDVFGWPLERLAFWMFGRQAKDVTLRYDEILENILRQHEDRGREEGLDREDRDLM 277

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILLKVYQD NAEF ITRTNIKAFLLDLF+GGTGTSTE++QW M+ELLNHPK F KLRNE
Sbjct: 278 DILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGTSTEVSQWTMAELLNHPKVFNKLRNE 337

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           INS+VGT+RLVGEDD+PNLPYLQAVVKE LRLYP VP+AMR CR+DCTIDG+ I K TMV
Sbjct: 338 INSIVGTSRLVGEDDLPNLPYLQAVVKEALRLYPAVPIAMRTCRQDCTIDGYHIPKDTMV 397

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFS 361
           AVNLF IM DPK W+N +EFDPERF  GD +YE KGQQSF FVPFGGGRRACPGSTLAFS
Sbjct: 398 AVNLFDIMRDPKIWKNSNEFDPERFT-GDVQYEFKGQQSFNFVPFGGGRRACPGSTLAFS 457

Query: 362 FVSTVVAAMVQCFDWKIGKNGDE-----TQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           F+S V+A MVQCFDWKI    D      ++V+MEI AAFTLP ANPL CVP++R NPF
Sbjct: 458 FISNVIATMVQCFDWKIIGRSDNNRDGISKVDMEIGAAFTLPMANPLRCVPIVRFNPF 513

BLAST of Lcy02g004290 vs. TAIR 10
Match: AT1G50520.1 (cytochrome P450, family 705, subfamily A, polypeptide 27 )

HSP 1 Score: 371.7 bits (953), Expect = 7.7e-103
Identity = 188/407 (46.19%), Postives = 261/407 (64.13%), Query Frame = 0

Query: 14  LPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFLRKLADSGEKNE 73
           L +   GF  A YG YWR+MKKL +T L     +E++R +R +E+  F   L D   K  
Sbjct: 122 LLFGSFGFVSAPYGDYWRFMKKLLVTNLFGSHSLEQTRLIREKELKTFRTMLFDKAAKKG 181

Query: 74  ALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIATKVSFGNVFGWP 133
            +D+G E++KLTNNSICR++M  RCS   E++EAEK+  LV ++F +  KV   N  G  
Sbjct: 182 TVDVGKEMMKLTNNSICRMIMGRRCS--EENSEAEKVEDLVIKSFSLVKKVLIANTVGRL 241

Query: 134 LERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMDILLKVYQDENA 193
           L++    LF ++  +V+ RYDE+L+KI+++HEE   +    +EDRD+MD+LL+V  D+ A
Sbjct: 242 LKKFGISLFEKEIMEVSQRYDELLEKIIKEHEEDPNK----KEDRDMMDVLLEVCADDKA 301

Query: 194 EFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEINSVVGTTRLVG 253
           E KITR  IKA +++LF+GGT TS +  QW+M+EL+NHP+    LR EI SVVGTTR + 
Sbjct: 302 EVKITRNQIKALIVELFLGGTDTSAQTIQWIMAELINHPEILKILREEIESVVGTTRFIQ 361

Query: 254 EDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVAVNLFAIMHDPK 313
           E D+ NLPYLQAV+KE  RL+P  P+ +R   + C I G+ I + T + +N +A+M DP 
Sbjct: 362 ETDLSNLPYLQAVMKEGQRLHPHSPMLVRNATKGCKIGGYYIPQNTTMLINTYAMMIDPD 421

Query: 314 TWQNPDEFDPERFI-----GGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSFVSTVVA 373
           +W+NPD+F PERF+     G D+  E   Q +  F+PFG GRRACPG  L + F    + 
Sbjct: 422 SWENPDKFQPERFMVSPSKGKDDERE---QLALNFIPFGSGRRACPGEKLGYLFTGVAIG 481

Query: 374 AMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPFA 416
            MVQCFDW I    D  +VN+E A   TL  A+PL C PV R+NP A
Sbjct: 482 TMVQCFDWII----DGDKVNVEEAGEMTLTMAHPLKCTPVTRVNPLA 515

BLAST of Lcy02g004290 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 370.2 bits (949), Expect = 2.2e-102
Identity = 194/419 (46.30%), Postives = 279/419 (66.59%), Query Frame = 0

Query: 2   FSSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKF 61
           FSSRP+F  A+   Y  + F  A+YG YWR+MKKL MT+LL+  Q+E+   +R EE LK 
Sbjct: 105 FSSRPEFGSAEYFKYRGSRFVLAQYGDYWRFMKKLCMTKLLAVPQLEKFADIREEEKLKL 164

Query: 62  LRKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIA 121
           +  +A    +    DL ++ IK TNN ICR+ MSTRCS  G DNEAE+IR LVK++ E+A
Sbjct: 165 VDSVAKCCREGLPCDLSSQFIKYTNNVICRMAMSTRCS--GTDNEAEEIRELVKKSLELA 224

Query: 122 TKVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLM 181
            K+S G+V G PL+ + F   G++   V  +YD ++++I+++ E +AK++  D   +D++
Sbjct: 225 GKISVGDVLG-PLKVMDFSGNGKKLVAVMEKYDLLVERIMKEREAKAKKK--DGTRKDIL 284

Query: 182 DILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNE 241
           DILL+ Y+D  AE KITR ++K+FLLD+F+ GT TS    QW M +L+NHP+AF KLR E
Sbjct: 285 DILLETYRDPTAEMKITRNDMKSFLLDVFMAGTDTSAAAMQWAMGQLINHPQAFNKLREE 344

Query: 242 INSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMV 301
           IN+VVG+ RLV E D+PNLPYL+AV++ETLRL+P  PL +R C EDC ++G  +   T V
Sbjct: 345 INNVVGSKRLVKESDVPNLPYLRAVLRETLRLHPSAPLIIRECAEDCQVNGCLVKSKTRV 404

Query: 302 AVNLFAIMHDPKTWQNPDEFDPERFIG------GDERYEIKGQQSFKFVPFGGGRRACPG 361
            VN++AIM D + W + D F PERF+       G+ + + KG Q+F+++PFG GRR CPG
Sbjct: 405 LVNVYAIMRDSELWADADRFIPERFLESSEEKIGEHQMQFKG-QNFRYLPFGSGRRGCPG 464

Query: 362 STLAFSFVSTVVAAMVQCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNPF 415
           ++LA + +   V ++VQ FDW   K+ D  +V++   + F+   A PL C PV   N F
Sbjct: 465 ASLAMNVMHIGVGSLVQRFDW---KSVDGQKVDLSQGSGFSAEMARPLVCNPVDHFNTF 514

BLAST of Lcy02g004290 vs. TAIR 10
Match: AT3G20960.1 (cytochrome P450, family 705, subfamily A, polypeptide 33 )

HSP 1 Score: 364.8 bits (935), Expect = 9.4e-101
Identity = 188/418 (44.98%), Postives = 270/418 (64.59%), Query Frame = 0

Query: 3   SSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFL 62
           SSR   A  + L +  +G   A YG Y +++KK+  T+LL  + +ERSRG+R EE+ +  
Sbjct: 4   SSRGAAAIDESLVFGSSGVVYAPYGDYLKFVKKIIATKLLRPQVLERSRGLRAEELQRLY 63

Query: 63  RKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIAT 122
            ++ D  + NE +++G E   L NN  CR+ M    S   E+ EAE++R LV E++ +A 
Sbjct: 64  NRILDKAKMNENVEIGKEATMLMNNIFCRMSMGR--SFSEENGEAERVRGLVAESYALAK 123

Query: 123 KVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMD 182
           K+ F +V    L++L   LF +   DV+ R++E+L+KIL +H E+  EE    +D D+MD
Sbjct: 124 KIFFASVLRRLLKKLRIPLFKKDIMDVSNRFNELLEKILVEHNEKLDEE---HKDTDMMD 183

Query: 183 ILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEI 242
           +LL  Y DENAE+KITR +IK+F ++LFVGGT TS + TQW M+E++N+     +LR EI
Sbjct: 184 VLLAAYADENAEYKITRNHIKSFFVELFVGGTDTSVQTTQWTMAEIINNSDVLERLREEI 243

Query: 243 NSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVA 302
           +SVVGT+R++ E DIPNLPYLQAVVKE LRL+PP PL  R   E C I GF I + T + 
Sbjct: 244 DSVVGTSRMIQETDIPNLPYLQAVVKEGLRLHPPFPLLTRKFEERCEIKGFYIPEKTFLI 303

Query: 303 VNLFAIMHDPKTWQNPDEFDPERFIGGDERYEI-KGQQSFKFVPFGGGRRACPGSTLAFS 362
           +N +A M DP +W++P+EF PERF+G     ++ + +++ K++PFGGGRR CPG+ LA  
Sbjct: 304 INAYAWMRDPDSWEDPNEFKPERFLGSSRLGQVDEREEAQKYIPFGGGRRGCPGANLASI 363

Query: 363 FVSTVVAAMVQCFDWKIGKNGDETQVNM-EIAAAFTLPRANPLDCVPVIRLNPFALAN 419
           FV T +  MVQCFDW  G  GD  ++NM E     TL   +P+ C P+ R  PF  +N
Sbjct: 364 FVGTAIGVMVQCFDW--GIKGD--KINMEETFEGLTLTMVHPIKCTPIPRTLPFVTSN 412

BLAST of Lcy02g004290 vs. TAIR 10
Match: AT4G15360.1 (cytochrome P450, family 705, subfamily A, polypeptide 3 )

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-100
Identity = 184/415 (44.34%), Postives = 268/415 (64.58%), Query Frame = 0

Query: 3   SSRPKFAFADELPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFL 62
           SSR   A  + L +  +G   A YG YW++MKKL  T+LL  + +ERSRGVR EE+ +F 
Sbjct: 106 SSRGIIALDESLMFGASGILNAPYGDYWKFMKKLMATKLLRPQVLERSRGVRVEELHRFY 165

Query: 63  RKLADSGEKNEALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIAT 122
           R + D   KNE++++G E +KL NN++C+L+M    S D  + E+ ++R LV ET+ ++ 
Sbjct: 166 RSILDKATKNESVEIGKEAMKLMNNTLCKLIMGRSFSED--NGESNRVRGLVDETYALSE 225

Query: 123 KVSFGNVFGWPLERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMD 182
           K+    +   PL +L   LF ++   V+ ++DE+L++IL++ +E  +E+  + E  D+MD
Sbjct: 226 KIFLAAILRRPLAKLRISLFKKEIMGVSNKFDELLERILQERKENLEEK--NNEGMDMMD 285

Query: 183 ILLKVYQDENAEFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEI 242
           +LL+ Y DENAE+KIT  +IKAF ++ F+GGT TS + TQW M+E++N+     +LR EI
Sbjct: 286 VLLEAYGDENAEYKITWKHIKAFFVEFFIGGTDTSVQTTQWAMAEMINNANVLERLREEI 345

Query: 243 NSVVGTTRLVGEDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVA 302
            SVVG TRL+ E D+PNLPYLQAVVKE LRL+PP P+ +R  +E C + GF I + T + 
Sbjct: 346 VSVVGETRLIQETDLPNLPYLQAVVKEVLRLHPPSPVLIRKFQEKCEVKGFYIPEKTTLI 405

Query: 303 VNLFAIMHDPKTWQNPDEFDPERFIGGDERYEIKGQQSFKFVPFGGGRRACPGSTLAFSF 362
           VN++AIM D  +W++P++F PERF+      E + ++  KF+PFG GRR CPG+ L   F
Sbjct: 406 VNVYAIMRDSDSWEDPEKFKPERFLTSSRSGE-EDEKELKFLPFGSGRRGCPGANLGSIF 465

Query: 363 VSTVVAAMVQCFDWKIGKNGDETQVNM-EIAAAFTLPRANPLDCVPVIRLNPFAL 417
           V T +  MVQCFDWKI     E +VNM E     TL   +PL C P    N + L
Sbjct: 466 VGTAIGVMVQCFDWKI----KEDKVNMEETFEGMTLKMVHPLTCTPFFEPNLYLL 511

BLAST of Lcy02g004290 vs. TAIR 10
Match: AT1G50560.1 (cytochrome P450, family 705, subfamily A, polypeptide 25 )

HSP 1 Score: 363.6 bits (932), Expect = 2.1e-100
Identity = 181/402 (45.02%), Postives = 259/402 (64.43%), Query Frame = 0

Query: 14  LPYAKAGFFGAEYGSYWRYMKKLTMTELLSQRQVERSRGVRREEMLKFLRKLADSGEKNE 73
           L +   GF    YG YWR+MKKL + +LL    +E++R +R +E+  F   L D   KNE
Sbjct: 123 LLFGSFGFVSVPYGDYWRFMKKLLVKKLLGSHSLEQTRLLRGKELQTFRAMLFDKAAKNE 182

Query: 74  ALDLGAELIKLTNNSICRLVMSTRCSGDGEDNEAEKIRTLVKETFEIATKVSFGNVFGWP 133
            +D+G E++KLTNNSICR+ M   CS   E+ EAE++R LV ++  +  K    ++ G  
Sbjct: 183 TVDVGKEMMKLTNNSICRMTMGRSCS--EENGEAEQVRGLVTKSLSLTKKFLIASIVGQF 242

Query: 134 LERLAFWLFGRQARDVTLRYDEILDKILRQHEERAKEEGLDREDRDLMDILLKVYQDENA 193
            + +   LFG++  +V+ RYDE+L+KI+++HEE       + EDRD+MD+LL+V  D+NA
Sbjct: 243 SKLVGISLFGKEIMEVSQRYDELLEKIIKEHEENPN----NGEDRDMMDVLLEVCADDNA 302

Query: 194 EFKITRTNIKAFLLDLFVGGTGTSTEITQWVMSELLNHPKAFAKLRNEINSVVGTTRLVG 253
           EFKI+R  IKA  +++F+ GT TS +  QW+++EL+NHP+   KLR EI SVVG  RL+ 
Sbjct: 303 EFKISRNQIKALFVEIFLAGTDTSAQTIQWILAELINHPEILEKLRKEIESVVGVRRLIQ 362

Query: 254 EDDIPNLPYLQAVVKETLRLYPPVPLAMRACREDCTIDGFDIAKGTMVAVNLFAIMHDPK 313
           E D+PNLPYLQAV+KE LRL+P  P+ +R   E C I G+ I + T + VN +A++ DP 
Sbjct: 363 ETDLPNLPYLQAVMKEGLRLHPHTPILVRNATEGCKIGGYYIGQNTTMMVNAYAVLRDPD 422

Query: 314 TWQNPDEFDPERFIGG--DERYEIKGQQSFKFVPFGGGRRACPGSTLAFSFVSTVVAAMV 373
           +W+ P+EF PERF+      + + K Q +  F+PFG GRR C G  L + F+   +  MV
Sbjct: 423 SWEYPEEFQPERFMTSPLKGKEDEKAQLALNFIPFGSGRRGCLGKNLGYIFMGVAIGTMV 482

Query: 374 QCFDWKIGKNGDETQVNMEIAAAFTLPRANPLDCVPVIRLNP 414
           Q FDW+I  NGD  +VNME     TL  A+PL C+PV R+NP
Sbjct: 483 QGFDWRI--NGD--KVNMEETGEMTLTMAHPLKCIPVARINP 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJY71.3e-9444.17Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1[more]
Q9FI391.9e-9343.90Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1[more]
Q9FH678.6e-9142.89Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1[more]
Q427981.5e-9043.363,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1... [more]
O819737.3e-9042.11Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DFG81.5e-19982.01cytochrome P450 705A5-like OS=Momordica charantia OX=3673 GN=LOC111020330 PE=3 S... [more]
A0A6J1GBA31.8e-19580.29cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111452598 PE=3 S... [more]
A0A5A7U3V62.8e-19379.90Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1K7Z61.4e-19279.57cytochrome P450 705A22-like OS=Cucurbita maxima OX=3661 GN=LOC111493062 PE=3 SV=... [more]
A0A6J1IYT75.3e-19282.15cytochrome P450 705A5-like OS=Cucurbita maxima OX=3661 GN=LOC111481896 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022152668.13.2e-19982.01cytochrome P450 705A5-like [Momordica charantia][more]
XP_023525669.12.3e-19781.01cytochrome P450 705A22-like [Cucurbita pepo subsp. pepo][more]
XP_038905910.11.9e-19682.69cytochrome P450 705A5-like [Benincasa hispida][more]
XP_022949167.13.6e-19580.29cytochrome P450 705A22-like [Cucurbita moschata][more]
KAA0048325.15.8e-19379.90cytochrome P450 705A5-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G50520.17.7e-10346.19cytochrome P450, family 705, subfamily A, polypeptide 27 [more]
AT2G42250.12.2e-10246.30cytochrome P450, family 712, subfamily A, polypeptide 1 [more]
AT3G20960.19.4e-10144.98cytochrome P450, family 705, subfamily A, polypeptide 33 [more]
AT4G15360.11.6e-10044.34cytochrome P450, family 705, subfamily A, polypeptide 3 [more]
AT1G50560.12.1e-10045.02cytochrome P450, family 705, subfamily A, polypeptide 25 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 304..328
score: 40.81
coord: 84..102
score: 22.62
coord: 221..247
score: 31.34
coord: 343..353
score: 55.9
coord: 201..218
score: 35.12
coord: 264..282
score: 35.21
coord: 353..376
score: 27.55
IPR001128Cytochrome P450PRINTSPR00385P450coord: 265..276
score: 51.03
coord: 344..353
score: 57.4
coord: 212..229
score: 24.6
coord: 353..364
score: 32.24
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..388
e-value: 6.3E-82
score: 275.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..410
e-value: 8.8E-99
score: 333.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..410
NoneNo IPR availablePANTHERPTHR24298:SF59CYTOCHROME P450, FAMILY 705, SUBFAMILY A, POLYPEPTIDE 25-RELATEDcoord: 2..414
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 2..414
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 346..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g004290.1Lcy02g004290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen