Lcy02g004080 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g004080
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Pre-mRNA-splicing factor ISY1-like protein
Location	Chr02: 5406691 .. 5407502 (+)
RNA-Seq Expression	Lcy02g004080
Synteny	Lcy02g004080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCACCCCTCACCGCCATACCCTACATGGAACTACTCCAAAAGATCTATATCCATTGACAGAAAAATCTTCTCCATTGCCTTTGATGAACAATCTCATGGAAGTCGGGCTTTGATCCACGAGATTGGCCGTAATTCCACACACTACCTCTCTGTTACTTGGAAGTCCCTTCAATGGTTGGCCTCATCATTCAACTCTCTCGCCAAGGACCCCTGTTCATACAAGTATTCTCTTAGGAAAGATCCGATGACTATGTGCACTGGATAGAAAAACTAAGGAAGAAGAATGGTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGATCCGGCTGGAGAAGCTCCATTGGTGCAATAGAGCTAGAAAGTCTCTTCAACAGACGGAACCAGGGTTTCTTCTGGTGCGACTCGTCCGCCAAATTTGATCAGGAAATTGTTCGGCGGCCAAATTCTTCAGGTGCAGATTTTTTAGGGTAGGATAAGATGGCTCGAAATGAGGAGAAGGCACAGTCAATGCTAAATCGTTTCATAGCCTTAAAAGCTGAGGAGAAGAAGAAACCTAAGCCGACCTTACCTTGCCTCTGAGTGTTGTGACCTTGCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGGGAGATTGGTCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTGGGTGAGCACCGCTTGCGTGATCTAAATGATGAGATTAACAAGCTCATCAGAGAGAAGTCACATTGGGAGCGCCGGATAGTTGGAGCTAGGTGGCCCCAATTATACCAAACACTCAGCCAAAATGACTGA

mRNA sequence

ATGAACCACCCCTCACCGCCATACCCTACATGGAACTACTCCAAAAGATCTATATCCATTGACAGAAAAATCTTCTCCATTGCCTTTGATGAACAATCTCATGGAAGTCGGGCTTTGATCCACGAGATTGGCCGTAATTCCACACACTACCTCTCTGTTACTTGGAAGTCCCTTCAATGGTTGGCCTCATCATTCAACTCTCTCGCCAAGGACCCCTGTTCATACAAACGGAACCAGGGTTTCTTCTGGTGCGACTCGTCCGCCAAATTTGATCAGGAAATTGTTCGGCGGCCAAATTCTTCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGGGAGATTGGTCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTGGGTGAGCACCGCTTGCGTGATCTAAATGATGAGATTAACAAGCTCATCAGAGAGAAGTCACATTGGGAGCGCCGGATAGTTGGAGCTAGGTGGCCCCAATTATACCAAACACTCAGCCAAAATGACTGA

Coding sequence (CDS)

ATGAACCACCCCTCACCGCCATACCCTACATGGAACTACTCCAAAAGATCTATATCCATTGACAGAAAAATCTTCTCCATTGCCTTTGATGAACAATCTCATGGAAGTCGGGCTTTGATCCACGAGATTGGCCGTAATTCCACACACTACCTCTCTGTTACTTGGAAGTCCCTTCAATGGTTGGCCTCATCATTCAACTCTCTCGCCAAGGACCCCTGTTCATACAAACGGAACCAGGGTTTCTTCTGGTGCGACTCGTCCGCCAAATTTGATCAGGAAATTGTTCGGCGGCCAAATTCTTCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGGGAGATTGGTCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTGGGTGAGCACCGCTTGCGTGATCTAAATGATGAGATTAACAAGCTCATCAGAGAGAAGTCACATTGGGAGCGCCGGATAGTTGGAGCTAGGTGGCCCCAATTATACCAAACACTCAGCCAAAATGACTGA

Protein sequence

MNHPSPPYPTWNYSKRSISIDRKIFSIAFDEQSHGSRALIHEIGRNSTHYLSVTWKSLQWLASSFNSLAKDPCSYKRNQGFFWCDSSAKFDQEIVRRPNSSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVGARWPQLYQTLSQND

Homology

[Close]

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot
Match: Q9ULR0 (Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 37/51 (72.55%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 102 EADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI 153
           +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI
Sbjct: 41  KAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRI 91

[Close]

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot
Match: Q69ZQ2 (Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 37/51 (72.55%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 102 EADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI 153
           +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI
Sbjct: 41  KAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRI 91

[Close]

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot
Match: Q6AYB3 (Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 37/51 (72.55%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 102 EADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI 153
           +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI
Sbjct: 41  KAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRI 91

[Close]

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot
Match: Q54N41 (Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=isy1 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.9e-13
Identity = 33/52 (63.46%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 102 EADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           +A+KWR+QI++EI R + EIQN  L E+++RDLND INKL+REK HWERRI+
Sbjct: 41  DAEKWRRQILKEITRGITEIQNSALDEYKIRDLNDHINKLVREKGHWERRIL 92

[Close]

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot
Match: Q4PEZ0 (Pre-mRNA-splicing factor ISY1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=ISY1 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.5e-12
Identity = 31/52 (59.62%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 102 EADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           E ++WR  ++REI RKV++IQ+ GL ++ +RDLNDEINKL+REK HWE +IV
Sbjct: 44  ECERWRSDVIREISRKVSKIQDFGLNDYEVRDLNDEINKLLREKGHWENQIV 95

[Close]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL
Match: A0A6A4QRS9 (Putative pre-mRNA-splicing factor Isy1 OS=Lupinus albus OX=3870 GN=Lal_00034101 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.7e-21
Identity = 56/71 (78.87%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV---GARW 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRE SHWERRIV   G  +
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRENSHWERRIVELGGPNY 100

Query: 161 PQLYQTLSQND 169
           P+L   ++  D
Sbjct: 101 PKLSAKMTDLD 111

[Close]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL
Match: A0A6A5PI33 (Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00035182 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.7e-21
Identity = 56/71 (78.87%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV---GARW 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRE SHWERRIV   G  +
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRENSHWERRIVELGGPNY 100

Query: 161 PQLYQTLSQND 169
           P+L   ++  D
Sbjct: 101 PKLSAKMTDLD 111

[Close]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL
Match: A0A371FXN8 (Pre-mRNA-splicing factor ISY1-like protein (Fragment) OS=Mucuna pruriens OX=157652 GN=Isy1 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.5e-20
Identity = 54/60 (90.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVGARWPQL 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+    P L
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRILELGGPNL 100

[Close]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL
Match: V7B7K9 (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_008G2330001g PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-20
Identity = 53/55 (96.36%), Postives = 54/55 (98.18%), Query Frame = 0


Query: 99  NSSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           + SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV
Sbjct: 39  DQSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 93

[Close]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL
Match: V7BPM7 (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_006G049400g PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-20
Identity = 53/55 (96.36%), Postives = 54/55 (98.18%), Query Frame = 0


Query: 99  NSSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           + SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV
Sbjct: 22  DQSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 76

[Close]

BLAST of Lcy02g004080 vs. NCBI nr
Match: TKY66561.1 (Pre-mRNA-splicing factor ISY1-like [Spatholobus suberectus])

HSP 1 Score: 110.5 bits (275), Expect = 1.4e-20
Identity = 55/60 (91.67%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVGARWPQL 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV    P L
Sbjct: 84  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNL 143

[Close]

BLAST of Lcy02g004080 vs. NCBI nr
Match: KAF1897476.1 (hypothetical protein Lal_00035182 [Lupinus albus])

HSP 1 Score: 110.5 bits (275), Expect = 1.4e-20
Identity = 56/71 (78.87%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV---GARW 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRE SHWERRIV   G  +
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRENSHWERRIVELGGPNY 100

Query: 161 PQLYQTLSQND 169
           P+L   ++  D
Sbjct: 101 PKLSAKMTDLD 111

[Close]

BLAST of Lcy02g004080 vs. NCBI nr
Match: KAE9617012.1 (putative pre-mRNA-splicing factor Isy1 [Lupinus albus] >KAE9617018.1 putative pre-mRNA-splicing factor Isy1 [Lupinus albus] >KAE9617019.1 putative pre-mRNA-splicing factor Isy1 [Lupinus albus] >KAF1896403.1 hypothetical protein Lal_00034101 [Lupinus albus])

HSP 1 Score: 110.5 bits (275), Expect = 1.4e-20
Identity = 56/71 (78.87%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV---GARW 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRE SHWERRIV   G  +
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIRENSHWERRIVELGGPNY 100

Query: 161 PQLYQTLSQND 169
           P+L   ++  D
Sbjct: 101 PKLSAKMTDLD 111

[Close]

BLAST of Lcy02g004080 vs. NCBI nr
Match: RDX83041.1 (Pre-mRNA-splicing factor ISY1-like protein, partial [Mucuna pruriens])

HSP 1 Score: 109.4 bits (272), Expect = 3.1e-20
Identity = 54/60 (90.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVGARWPQL 160
           SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+    P L
Sbjct: 41  SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRILELGGPNL 100

[Close]

BLAST of Lcy02g004080 vs. NCBI nr
Match: XP_007141873.1 (hypothetical protein PHAVU_008G2330001g [Phaseolus vulgaris] >ESW13867.1 hypothetical protein PHAVU_008G2330001g [Phaseolus vulgaris])

HSP 1 Score: 109.0 bits (271), Expect = 4.0e-20
Identity = 53/55 (96.36%), Postives = 54/55 (98.18%), Query Frame = 0


Query: 99  NSSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           + SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV
Sbjct: 39  DQSEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 93

[Close]

BLAST of Lcy02g004080 vs. TAIR 10
Match: AT3G18790.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Isy1-like splicing (InterPro:IPR009360); Has 1147 Blast hits to 965 proteins in 236 species: Archae - 12; Bacteria - 13; Metazoa - 351; Fungi - 230; Plants - 49; Viruses - 9; Other Eukaryotes - 483 (source: NCBI BLink). )

HSP 1 Score: 99.8 bits (247), Expect = 2.3e-21
Identity = 47/53 (88.68%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 101 SEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIV 154
           +EADKWRQQI+REIG KVAEIQNEGLGEHRLRDLNDEINKL+RE+ HWERRIV
Sbjct: 41  AEADKWRQQILREIGSKVAEIQNEGLGEHRLRDLNDEINKLLRERYHWERRIV 93

The following BLAST results are available for this feature:

BLAST of Lcy02g004080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ULR0	3.4e-14	72.55	Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3	[more]
Q69ZQ2	3.4e-14	72.55	Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2	[more]
Q6AYB3	3.4e-14	72.55	Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2...	[more]
Q54N41	2.9e-13	63.46	Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=is...	[more]
Q4PEZ0	5.5e-12	59.62	Pre-mRNA-splicing factor ISY1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237...	[more]

BLAST of Lcy02g004080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6A4QRS9	6.7e-21	78.87	Putative pre-mRNA-splicing factor Isy1 OS=Lupinus albus OX=3870 GN=Lal_00034101 ...	[more]
A0A6A5PI33	6.7e-21	78.87	Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00035182 PE=3 SV=1	[more]
A0A371FXN8	1.5e-20	90.00	Pre-mRNA-splicing factor ISY1-like protein (Fragment) OS=Mucuna pruriens OX=1576...	[more]
V7B7K9	2.0e-20	96.36	Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_008G2330001g PE=3...	[more]
V7BPM7	2.0e-20	96.36	Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_006G049400g PE=3 ...	[more]

BLAST of Lcy02g004080 vs. NCBI nr


Match Name	E-value	Identity	Description
TKY66561.1	1.4e-20	91.67	Pre-mRNA-splicing factor ISY1-like [Spatholobus suberectus]	[more]
KAF1897476.1	1.4e-20	78.87	hypothetical protein Lal_00035182 [Lupinus albus]	[more]
KAE9617012.1	1.4e-20	78.87	putative pre-mRNA-splicing factor Isy1 [Lupinus albus] >KAE9617018.1 putative pr...	[more]
RDX83041.1	3.1e-20	90.00	Pre-mRNA-splicing factor ISY1-like protein, partial [Mucuna pruriens]	[more]
XP_007141873.1	4.0e-20	96.36	hypothetical protein PHAVU_008G2330001g [Phaseolus vulgaris] >ESW13867.1 hypothe...	[more]

BLAST of Lcy02g004080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18790.1	2.3e-21	88.68	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 131..151
IPR009360	Pre-mRNA-splicing factor Isy1	PFAM	PF06246	Isy1	coord: 100..153 e-value: 3.0E-25 score: 89.3
IPR009360	Pre-mRNA-splicing factor Isy1	PANTHER	PTHR13021	PRE-MRNA-SPLICING FACTOR ISY1	coord: 100..153
IPR029012	Helix hairpin bin domain superfamily	GENE3D	1.10.287.660	Helix hairpin bin	coord: 101..154 e-value: 9.5E-28 score: 97.8
IPR037200	Pre-mRNA-splicing factor Isy1 superfamily	SUPERFAMILY	140102	ISY1 domain-like	coord: 99..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g004080.1	Lcy02g004080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000350	generation of catalytic spliceosome for second transesterification step
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus