Lcy02g004060 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g004060
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNA/RNP complex-1-interacting phosphatase, putative
Location	Chr02: 5401351 .. 5402097 (+)
RNA-Seq Expression	Lcy02g004060
Synteny	Lcy02g004060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAATATCTCCAAACCCCATTTCTTCCCCTCCTTCCATTTCCATCATTCCATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCGCCGCCAAAGCGACGGCGGCGCCGAGCAATTCAGAAACAGAATCAGCAGCCCCAGGTCGATCGCCCTCCTCTGCCGCCACAGCCAAGATCCTTCAGATTCTTCAGAAGAAAAAACAAAGAAAAGCCCAGAAAGGAAACAGAAGATTCCTGGGTTTGTTCAGAGGTTTGAGAAGATGGGAATGGAATTGAAGGAGAAATTGAGTCCTCAGAGGAAAGGGGACTGGAAGGATTTGACATTGATGAGCTTGTCATTTGCTGTTTATGTGTATATATCTCAGAAGATTGTTTGTGCTTATTTTGTTTGGATGTCAATGCCAAAACAACTGTGGTAGAATTTAGAAGATGATGGTTTTCAGAGTGAGGATTTGTCAGATCTTCTTGGTGTGGTTGTATAATTGGTCTTTTTGTTTGTTTGAGATTTGTAGAAATTAAATTGTTGAGATCAATTCAGTTCTTTATAATGTTTATGTAGTTAAATTATAATTTTATTTTCTAAGCTGATATGATATGGTTCTGATCTCTATCTTCTATCTTTAGATTAAAGTTTGTTCTATATTCTTGAAATAGAAATGGCAATAGAATTATAAGTCGGTTTTAAGTCGGTTTTGACGAGTTACTTGCTAAGGTATAAATTTGCTTTATAATATGAAATGAAC

mRNA sequence

AAAAAAAATATCTCCAAACCCCATTTCTTCCCCTCCTTCCATTTCCATCATTCCATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCGCCGCCAAAGCGACGGCGGCGCCGAGCAATTCAGAAACAGAATCAGCAGCCCCAGGTCGATCGCCCTCCTCTGCCGCCACAGCCAAGATCCTTCAGATTCTTCAGAAGAAAAAACAAAGAAAAGCCCAGAAAGGAAACAGAAGATTCCTGGGTTTGTTCAGAGGTTTGAGAAGATGGGAATGGAATTGAAGGAGAAATTGAGTCCTCAGAGGAAAGGGGACTGGAAGGATTTGACATTGATGAGCTTGTCATTTGCTGTTTATGTGTATATATCTCAGAAGATTGTTTGTGCTTATTTTGTTTGGATGTCAATGCCAAAACAACTGTGGTAGAATTTAGAAGATGATGGTTTTCAGAGTGAGGATTTGTCAGATCTTCTTGGTGTGGTTGTATAATTGGTCTTTTTGTTTGTTTGAGATTTGTAGAAATTAAATTGTTGAGATCAATTCAGTTCTTTATAATGTTTATGTAGTTAAATTATAATTTTATTTTCTAAGCTGATATGATATGGTTCTGATCTCTATCTTCTATCTTTAGATTAAAGTTTGTTCTATATTCTTGAAATAGAAATGGCAATAGAATTATAAGTCGGTTTTAAGTCGGTTTTGACGAGTTACTTGCTAAGGTATAAATTTGCTTTATAATATGAAATGAAC

Coding sequence (CDS)

ATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCGCCGCCAAAGCGACGGCGGCGCCGAGCAATTCAGAAACAGAATCAGCAGCCCCAGGTCGATCGCCCTCCTCTGCCGCCACAGCCAAGATCCTTCAGATTCTTCAGAAGAAAAAACAAAGAAAAGCCCAGAAAGGAAACAGAAGATTCCTGGGTTTGTTCAGAGGTTTGAGAAGATGGGAATGGAATTGAAGGAGAAATTGAGTCCTCAGAGGAAAGGGGACTGGAAGGATTTGACATTGATGAGCTTGTCATTTGCTGTTTATGTGTATATATCTCAGAAGATTGTTTGTGCTTATTTTGTTTGGATGTCAATGCCAAAACAACTGTGGTAG

Protein sequence

MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPSDSSEEKTKKSPERKQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSMPKQLW

Homology

[Close]

BLAST of Lcy02g004060 vs. ExPASy TrEMBL
Match: A0A5D3BX35 (Putative RNA/RNP complex-1-interacting phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1871G00020 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.7e-43
Identity = 98/126 (77.78%), Postives = 106/126 (84.13%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPS---DSSEEKT-KKSPE 60
           MAALSAGHRHFF   S     QFRNR  SPRSIA+LC H+QDPS   D S+EKT KKS E
Sbjct: 1   MAALSAGHRHFFYSLSGSAIVQFRNRSISPRSIAVLCLHNQDPSDPTDCSDEKTKKKSQE 60

Query: 61  RKQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120
           RK+ + GFVQRFEKMG++LKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYF+WMS
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMS 120

Query: 121 MPKQLW 123
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Lcy02g004060 vs. ExPASy TrEMBL
Match: A0A0A0LD14 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G690300 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 5.9e-43
Identity = 97/126 (76.98%), Postives = 106/126 (84.13%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPS---DSSEEKT-KKSPE 60
           MAALSAGHRHFF  QS      FRNR  SPRSI++LC H+QDPS   D SEEKT KKS E
Sbjct: 1   MAALSAGHRHFFYSQSGSATLLFRNRSISPRSISVLCLHNQDPSDPTDCSEEKTKKKSQE 60

Query: 61  RKQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120
           RK+ + GFVQRFEKMG++LKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYF+WM+
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMT 120

Query: 121 MPKQLW 123
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Lcy02g004060 vs. ExPASy TrEMBL
Match: A0A6J1DGN1 (uncharacterized protein LOC111020315 OS=Momordica charantia OX=3673 GN=LOC111020315 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.5e-42
Identity = 99/124 (79.84%), Postives = 105/124 (84.68%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPSDSSEEKTKKSPERK-Q 60
           MAALSAGHRHFFRR     A+Q R R SSP+SI LLCRHSQDP   SEE +K+  ERK Q
Sbjct: 1   MAALSAGHRHFFRR--GAAAQQLRTRRSSPKSIVLLCRHSQDP---SEENSKEGEERKQQ 60

Query: 61  KIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSMPK 120
           KI GFV+RFEKMGMELKEKLSPQRKGDWKD+ LMSLSFAVYVYISQKIVCAYFVWMSMPK
Sbjct: 61  KIGGFVERFEKMGMELKEKLSPQRKGDWKDVALMSLSFAVYVYISQKIVCAYFVWMSMPK 119

Query: 121 -QLW 123
            QLW
Sbjct: 121 QQLW 119

[Close]

BLAST of Lcy02g004060 vs. ExPASy TrEMBL
Match: A0A6P9E117 (uncharacterized protein LOC118344507 OS=Juglans regia OX=51240 GN=LOC118344507 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.0e-23
Identity = 68/122 (55.74%), Postives = 80/122 (65.57%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPSDSSEEKTKKSPERKQK 60
           MAAL  GH   +RR       Q   + SSPR+  LL   S  P+D+S  +TKK   R+  
Sbjct: 1   MAALLVGH-SLYRRIC---TIQEPRKKSSPRTFILLGCQSHKPADTSNPRTKKESRRESL 60

Query: 61  IPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSMPKQ 120
           +PG +  F K G  LKE +SPQ+KGDWKD+ LMSLSFAVYVYISQKIVCAY  WMSMPKQ
Sbjct: 61  LPGILVGFGKFGKVLKENMSPQQKGDWKDVMLMSLSFAVYVYISQKIVCAYCAWMSMPKQ 118

Query: 121 LW 123
            W
Sbjct: 121 PW 118

[Close]

BLAST of Lcy02g004060 vs. ExPASy TrEMBL
Match: A0A660KVF0 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010795 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 8.9e-23
Identity = 70/126 (55.56%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPSDSSEEKTKKSPERKQK 60
           MAALS GH   F R+      Q   + SSP ++ L C     P DSS  +TKK   +++ 
Sbjct: 1   MAALSIGHS--FHREPI--TYQQLRKSSSPTTLILSCCQRHKPVDSSNPRTKKEKSKRKL 60

Query: 61  IPGFVQR----FEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120
           +   +QR    FEK G  L+E LSP++KGDWKDLTLMSLSFAVYVYISQK+VCAYF WMS
Sbjct: 61  L---LQRSSVGFEKFGKILRENLSPKQKGDWKDLTLMSLSFAVYVYISQKLVCAYFAWMS 119

Query: 121 MPKQLW 123
           MPKQLW
Sbjct: 121 MPKQLW 119

[Close]

BLAST of Lcy02g004060 vs. NCBI nr
Match: KAG6606884.1 (hypothetical protein SDJN03_00226, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036590.1 hypothetical protein SDJN02_00209, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 186.8 bits (473), Expect = 1.1e-43
Identity = 99/125 (79.20%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQ---DPSDSSEEKTKKSPER 60
           MAALSAGHR+FF       A  FRNR  SPRSI LLC H+Q   DPSD SEEK KK  E+
Sbjct: 1   MAALSAGHRYFF----GSAAVHFRNRSISPRSIVLLCLHNQNPSDPSDPSEEKPKKEQEK 60

Query: 61  KQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSM 120
           KQ IPGFVQRFEKMG+ELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSM
Sbjct: 61  KQSIPGFVQRFEKMGIELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSM 120

Query: 121 PKQLW 123
           PKQ+W
Sbjct: 121 PKQVW 121

[Close]

BLAST of Lcy02g004060 vs. NCBI nr
Match: KAG6581569.1 (hypothetical protein SDJN03_21571, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018076.1 hypothetical protein SDJN02_19942, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 185.7 bits (470), Expect = 2.5e-43
Identity = 97/124 (78.23%), Postives = 104/124 (83.87%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLC--RHSQDPSDSSEEKTKKSPERK 60
           MAALSAGHRHFF       A QF NR  SPR I LLC   H+QDPSDSSEEKTKK  E K
Sbjct: 1   MAALSAGHRHFF------PAVQFGNRSISPRPIVLLCCHNHNQDPSDSSEEKTKKDQEGK 60

Query: 61  QKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSMP 120
           Q +PGF+QR++KMGMELKEKLSPQRKGDWKD+TLMSLSFAVYVYISQKIVCAYF+WMSMP
Sbjct: 61  QMVPGFIQRYKKMGMELKEKLSPQRKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMP 118

Query: 121 KQLW 123
           KQLW
Sbjct: 121 KQLW 118

[Close]

BLAST of Lcy02g004060 vs. NCBI nr
Match: KAA0048367.1 (putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa] >TYK04067.1 putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa])

HSP 1 Score: 184.5 bits (467), Expect = 5.5e-43
Identity = 98/126 (77.78%), Postives = 106/126 (84.13%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPS---DSSEEKT-KKSPE 60
           MAALSAGHRHFF   S     QFRNR  SPRSIA+LC H+QDPS   D S+EKT KKS E
Sbjct: 1   MAALSAGHRHFFYSLSGSAIVQFRNRSISPRSIAVLCLHNQDPSDPTDCSDEKTKKKSQE 60

Query: 61  RKQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120
           RK+ + GFVQRFEKMG++LKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYF+WMS
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMS 120

Query: 121 MPKQLW 123
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Lcy02g004060 vs. NCBI nr
Match: KGN58582.1 (hypothetical protein Csa_001602 [Cucumis sativus])

HSP 1 Score: 183.3 bits (464), Expect = 1.2e-42
Identity = 97/126 (76.98%), Postives = 106/126 (84.13%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPS---DSSEEKT-KKSPE 60
           MAALSAGHRHFF  QS      FRNR  SPRSI++LC H+QDPS   D SEEKT KKS E
Sbjct: 1   MAALSAGHRHFFYSQSGSATLLFRNRSISPRSISVLCLHNQDPSDPTDCSEEKTKKKSQE 60

Query: 61  RKQKIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120
           RK+ + GFVQRFEKMG++LKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYF+WM+
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMT 120

Query: 121 MPKQLW 123
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Lcy02g004060 vs. NCBI nr
Match: XP_022152649.1 (uncharacterized protein LOC111020315 [Momordica charantia])

HSP 1 Score: 179.9 bits (455), Expect = 1.4e-41
Identity = 99/124 (79.84%), Postives = 105/124 (84.68%), Query Frame = 0


Query: 1   MAALSAGHRHFFRRQSDGGAEQFRNRISSPRSIALLCRHSQDPSDSSEEKTKKSPERK-Q 60
           MAALSAGHRHFFRR     A+Q R R SSP+SI LLCRHSQDP   SEE +K+  ERK Q
Sbjct: 1   MAALSAGHRHFFRR--GAAAQQLRTRRSSPKSIVLLCRHSQDP---SEENSKEGEERKQQ 60

Query: 61  KIPGFVQRFEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMSMPK 120
           KI GFV+RFEKMGMELKEKLSPQRKGDWKD+ LMSLSFAVYVYISQKIVCAYFVWMSMPK
Sbjct: 61  KIGGFVERFEKMGMELKEKLSPQRKGDWKDVALMSLSFAVYVYISQKIVCAYFVWMSMPK 119

Query: 121 -QLW 123
            QLW
Sbjct: 121 QQLW 119

[Close]

BLAST of Lcy02g004060 vs. TAIR 10
Match: AT3G52070.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 23 Blast hits to 23 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 63.9 bits (154), Expect = 1.0e-10
Identity = 42/80 (52.50%), Postives = 50/80 (62.50%), Query Frame = 0


Query: 57  RKQKIP----GFVQR--------FEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYIS 116
           RKQK+P    G +QR          K+G  LK +   ++KGD KDL LMSLSFAVYVYIS
Sbjct: 38  RKQKLPEENEGVIQRTLRRMISEAGKIGKNLKPE---KKKGDVKDLMLMSLSFAVYVYIS 97

Query: 117 QKIVCAYFVW--MSMPKQLW 123
           Q +VCAYF W  +S PK  W
Sbjct: 98  QLLVCAYFSWQHLSFPKSSW 114

[Close]

BLAST of Lcy02g004060 vs. TAIR 10
Match: AT3G52070.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 23 Blast hits to 23 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 60.8 bits (146), Expect = 8.5e-10
Identity = 41/77 (53.25%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 57  RKQKIP----GFVQR--------FEKMGMELKEKLSPQRKGDWKDLTLMSLSFAVYVYIS 116
           RKQK+P    G +QR          K+G  LK +   ++KGD KDL LMSLSFAVYVYIS
Sbjct: 38  RKQKLPEENEGVIQRTLRRMISEAGKIGKNLKPE---KKKGDVKDLMLMSLSFAVYVYIS 97

Query: 117 QKIVCAYFVW--MSMPK 120
           Q +VCAYF W  +S PK
Sbjct: 98  QLLVCAYFSWQHLSFPK 111

The following BLAST results are available for this feature:

BLAST of Lcy02g004060 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy02g004060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BX35	2.7e-43	77.78	Putative RNA/RNP complex-1-interacting phosphatase OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LD14	5.9e-43	76.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G690300 PE=4 SV=1	[more]
A0A6J1DGN1	6.5e-42	79.84	uncharacterized protein LOC111020315 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6P9E117	4.0e-23	55.74	uncharacterized protein LOC118344507 OS=Juglans regia OX=51240 GN=LOC118344507 P...	[more]
A0A660KVF0	8.9e-23	55.56	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010795 PE=4 SV=1	[more]

BLAST of Lcy02g004060 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606884.1	1.1e-43	79.20	hypothetical protein SDJN03_00226, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6581569.1	2.5e-43	78.23	hypothetical protein SDJN03_21571, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0048367.1	5.5e-43	77.78	putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa] >T...	[more]
KGN58582.1	1.2e-42	76.98	hypothetical protein Csa_001602 [Cucumis sativus]	[more]
XP_022152649.1	1.4e-41	79.84	uncharacterized protein LOC111020315 [Momordica charantia]	[more]

BLAST of Lcy02g004060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52070.1	1.0e-10	52.50	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G52070.2	8.5e-10	53.25	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g004060.1	Lcy02g004060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane