Lcy02g003010 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g003010
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionEpoxide hydrolase 2
LocationChr02: 4024071 .. 4025501 (-)
RNA-Seq ExpressionLcy02g003010
SyntenyLcy02g003010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAGAGACAGAGAGAAGAACAAGGCGGAGAAGAAACAAAACGAAGCCATCCATGGAGAAAATCGAGCACTCAACCATCGCCACCAATGGCATCAACATCCACGTCGCTTCCATCGGCTCCGGTCCGGCGGTGCTATTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTCCTCGCCTCCAAGGGCTTCCGAGCCATAGCCCCCGACCTCCGCGGCTTCGGCGACTCCGACGCGCCGCCGTCTCCCTCCTCCTACACGCCTCACCACATCATCGGCGACCTCATCGGCGTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTAGGGCACGATTGGGGCGCGATGATGGCCTGGTACTTTTGCCTTTTCAGGTCCGATCGAGTCAAGGCTCTGGTCAATTTGAGTGTCCATTTTACTCCTCGGTTTCCGGAAATGTCGCCTCTCAGCGCCCTCCGACAGTTCCTCGGCAACGATTTCTACATATGTAAATTTCAGGTGAACGCGATTGAATTTGAATCTTCGCACTCTTTTGTAATCTCTTAAATTGAATTGAATCGGTCGCATTAGGTCAACTGATGAATCGCTAAGTATGGATTTCTGCACTATACGTAACCTCAGAAATTGAATGAATTCGATCGCTTCAGATCGACTGCTGAACCGCTAATTGTGGATTTTGGCACAGGAACCTGGAGTAGCCGAAGCCGATTTTGGATCTGACAGTACGGCTACGATGATGAAAAAATTCCTAACGCTGAGAGATCCAAGCCCTCCGATTATACCAAACGGATTCAAAACTCTGCCGACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAGGATATCGATTATTTCGCCTCCAAATTCGCCAAAACTGGTTTCACCGGCGGATTCAACTACTATCGCGCCATTGACCTGTGAGGATTCACAACTTTTGCTTGTTGAAATCGATTTGTCTGTTCCTGTTATCTAGGGCCAAATAAGTAAGAAACAAATCTTGTGTGTTTCGTTGATTGATTGCAGAACATGGGAGCTCACAGGGCCATGGAGCGGATCAAAGATCAAAGTTCCGACGAAATTCATAGTCGGCGATCTCGATTTGGTGTACCATTTTCCAGGTGCGAAGGAGTACATTCACGACGGCGGATTCAAGGAGGACGTGCCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCATTTCATCAACCAGGAAAAAGCAGATGAAATAAGCTTCCTCATCTATGATTTCATCAAGAAATTCTGATTCCTTTCATGGCGATTGTGTAATAACCGTCTGTTTGGATTTCTTCAATGCTTAATCTCTTAAAAGTACTTTTTGAACATCATATCTGGCTACATTTTCCTTCTTTTCTGGCTATATTTTTCTTTTCTTTTTTTTAATTAG

mRNA sequence

CGCAGAGACAGAGAGAAGAACAAGGCGGAGAAGAAACAAAACGAAGCCATCCATGGAGAAAATCGAGCACTCAACCATCGCCACCAATGGCATCAACATCCACGTCGCTTCCATCGGCTCCGGTCCGGCGGTGCTATTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTCCTCGCCTCCAAGGGCTTCCGAGCCATAGCCCCCGACCTCCGCGGCTTCGGCGACTCCGACGCGCCGCCGTCTCCCTCCTCCTACACGCCTCACCACATCATCGGCGACCTCATCGGCGTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTAGGGCACGATTGGGGCGCGATGATGGCCTGGTACTTTTGCCTTTTCAGGTCCGATCGAGTCAAGGCTCTGGTCAATTTGAGTGTCCATTTTACTCCTCGGTTTCCGGAAATGTCGCCTCTCAGCGCCCTCCGACAGTTCCTCGGCAACGATTTCTACATATGTAAATTTCAGGAACCTGGAGTAGCCGAAGCCGATTTTGGATCTGACAGTACGGCTACGATGATGAAAAAATTCCTAACGCTGAGAGATCCAAGCCCTCCGATTATACCAAACGGATTCAAAACTCTGCCGACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAGGATATCGATTATTTCGCCTCCAAATTCGCCAAAACTGGTTTCACCGGCGGATTCAACTACTATCGCGCCATTGACCTAACATGGGAGCTCACAGGGCCATGGAGCGGATCAAAGATCAAAGTTCCGACGAAATTCATAGTCGGCGATCTCGATTTGGTGTACCATTTTCCAGGTGCGAAGGAGTACATTCACGACGGCGGATTCAAGGAGGACGTGCCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCATTTCATCAACCAGGAAAAAGCAGATGAAATAAGCTTCCTCATCTATGATTTCATCAAGAAATTCTGATTCCTTTCATGGCGATTGTGTAATAACCGTCTGTTTGGATTTCTTCAATGCTTAATCTCTTAAAAGTACTTTTTGAACATCATATCTGGCTACATTTTCCTTCTTTTCTGGCTATATTTTTCTTTTCTTTTTTTTAATTAG

Coding sequence (CDS)

ATGGAGAAAATCGAGCACTCAACCATCGCCACCAATGGCATCAACATCCACGTCGCTTCCATCGGCTCCGGTCCGGCGGTGCTATTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTCCTCGCCTCCAAGGGCTTCCGAGCCATAGCCCCCGACCTCCGCGGCTTCGGCGACTCCGACGCGCCGCCGTCTCCCTCCTCCTACACGCCTCACCACATCATCGGCGACCTCATCGGCGTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTAGGGCACGATTGGGGCGCGATGATGGCCTGGTACTTTTGCCTTTTCAGGTCCGATCGAGTCAAGGCTCTGGTCAATTTGAGTGTCCATTTTACTCCTCGGTTTCCGGAAATGTCGCCTCTCAGCGCCCTCCGACAGTTCCTCGGCAACGATTTCTACATATGTAAATTTCAGGAACCTGGAGTAGCCGAAGCCGATTTTGGATCTGACAGTACGGCTACGATGATGAAAAAATTCCTAACGCTGAGAGATCCAAGCCCTCCGATTATACCAAACGGATTCAAAACTCTGCCGACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAGGATATCGATTATTTCGCCTCCAAATTCGCCAAAACTGGTTTCACCGGCGGATTCAACTACTATCGCGCCATTGACCTAACATGGGAGCTCACAGGGCCATGGAGCGGATCAAAGATCAAAGTTCCGACGAAATTCATAGTCGGCGATCTCGATTTGGTGTACCATTTTCCAGGTGCGAAGGAGTACATTCACGACGGCGGATTCAAGGAGGACGTGCCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCATTTCATCAACCAGGAAAAAGCAGATGAAATAAGCTTCCTCATCTATGATTTCATCAAGAAATTCTGA

Protein sequence

MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRDPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKADEISFLIYDFIKKF
Homology
BLAST of Lcy02g003010 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-60
Identity = 126/318 (39.62%), Postives = 177/318 (55.66%), Query Frame = 0

Query: 9   IATNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSD 68
           + TNG+ + V   G    P V+  HGFPEL YSWRHQ+  LA  G+  +APD RG+G S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSV 128
            P +  +Y  H +  DL+G+LD +G ++   VGHDWGA++ W   L  +DRV A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 HFTPRFPEMSPLSALRQFLG-NDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRDPSPP 188
              PR  ++ P  A R   G N FYI  FQEPG+A+A+   D   TM +    LR P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 II--------PNGF-KTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWE 248
                     P+GF   LP P  LP W+++E++D++  +F +TGFTGG N+YR  D  WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTGPWSGSKIKVPTKFIVGDLDLVYHF---PGAKEYIHDGGFKEDVPLLEEVVVIEGAAH 308
            T   +G  I VP+ FI G  D V  F     A E I  G ++E        V+I+GA H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVI-SGPYRE--------VLIDGAGH 308

Query: 309 FINQEKADEISFLIYDFI 312
           ++ QE+  E++  + +F+
Sbjct: 309 WLQQERPGEVTAALLEFL 316

BLAST of Lcy02g003010 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 103/304 (33.88%), Postives = 162/304 (53.29%), Query Frame = 0

Query: 14  INIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPSS 73
           + +H   +GSGPAV   HGFPE WYSWR+Q+  LA  G+R +A D++G+G+S APP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSVHFTPRFP 133
           Y    +  +++  LD LG+ Q   +GHDWG M+ WY  LF  +RV+A+ +L+  F P  P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 EMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRDPS----PPIIPN 193
            MSPL +++     D+ +  FQEPGVAEA+   + + T  K      D S      +   
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAELEQNLSRT-FKSLFRASDESVLSMHKVCEA 427

Query: 194 G--FKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGPWSGSKIK 253
           G  F   P   +L   +TEE+I ++  +F K+GF G  N+YR ++  W+      G KI 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKADEISFLI 312
           +P   +  + D V   P   +++ D      +P L+    IE   H+   +K  E++ ++
Sbjct: 488 IPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKR-GHIEDCGHWTQMDKPTEVNQIL 542

BLAST of Lcy02g003010 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 8.9e-48
Identity = 105/307 (34.20%), Postives = 165/307 (53.75%), Query Frame = 0

Query: 13  GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           GI +H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR +A D++G+GDS +PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSVHFTPRF 132
            Y    +  +++  LD LGI Q   +GHDW  +M W   LF  +RV+A+ +L+  F P  
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRDPSPPIIPN--- 192
           P++SP+  +R     ++ +  FQEPGVAEA+   + + T  K F    D +  I  +   
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRT-FKSFFRASDETGFIAVHKAT 424

Query: 193 --GFKTLPTPE--TLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGPWSGSK 252
             G   + TPE   L    TEE+I+++  +F KTGF G  N+YR  +  W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKADEISF 312
           I VP   +  + D+V     +K        ++ +P L+    IE   H+   EK  E++ 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVNQ 542

BLAST of Lcy02g003010 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 8.9e-48
Identity = 96/256 (37.50%), Postives = 145/256 (56.64%), Query Frame = 0

Query: 13  GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           G+ +H   +GSGPAV   HGFPE W+SWR+Q+  LA  GFR +A D++G+G+S APP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSVHFTPRF 132
            Y+   +  D++  L+ LG+ Q   +GHDWG ++ W   LF  +RV+A+ +L+  F P  
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRD----PSPPIIP 192
           P +SP+  ++     D+ +  FQEPGVAEA+   +   T  K F    D     +  +  
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAELEQNLDRT-FKNFFRAHDETFLTTNRVRE 426

Query: 193 NGFKTLPTPE--TLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGPWSGSKI 252
            G   + TPE  +L   +TEEDI ++  +F K+GF G  N+YR ++  W+     SG KI
Sbjct: 427 LGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKI 486

Query: 253 KVPTKFIVGDLDLVYH 263
            +P   +  + DLV H
Sbjct: 487 LIPALMVTAENDLVLH 500

BLAST of Lcy02g003010 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.5e-47
Identity = 102/306 (33.33%), Postives = 161/306 (52.61%), Query Frame = 0

Query: 13  GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           GI +H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR +A D++G+GDS +PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKALVNLSVHFTPRF 132
            Y    +  +++  L+ LGI Q   +GHDW  ++ W   LF  +RV+A+ +L+    P  
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLRDPSPPIIPN--- 192
           PE+SP+  +R     ++ +  FQEPGVAEA+   + + T    F T  D     +     
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATE 424

Query: 193 -GFKTLPTPE--TLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGPWSGSKI 252
            G   + TPE   +    TEE+I+Y+  +F K+GF G  N+YR  +  W+ +    G KI
Sbjct: 425 MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKI 484

Query: 253 KVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKADEISFL 312
            VP   +  + D+V     +K        +  +P L+    IE   H+   EK  E++ +
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVNQI 542

BLAST of Lcy02g003010 vs. ExPASy TrEMBL
Match: A0A6J1J107 (uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.5e-167
Identity = 278/314 (88.54%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           ME+IEHSTIATNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P +SPLS  RQFLG+DFYICKFQ+PGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTKF+VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 301 DEISAHIYDFINKF 314

BLAST of Lcy02g003010 vs. ExPASy TrEMBL
Match: A0A6J1J7K4 (uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.5e-167
Identity = 278/314 (88.54%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           ME+IEHSTIATNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P +SPLS  RQFLG+DFYICKFQ+PGVAEADFGS  TATMMKKFLT+R
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 524

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 525 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 584

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTKF+VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 585 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 644

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 645 DEISAHIYDFINKF 658

BLAST of Lcy02g003010 vs. ExPASy TrEMBL
Match: A0A6J1F1K3 (uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC111441542 PE=4 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.4e-165
Identity = 277/314 (88.22%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           MEKIEHSTIATNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGI+QVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+ PR P +SPLS  RQFLG+DFYICKFQ PGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPETLP WLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTK++VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 301 DEISAHIYDFIIKF 314

BLAST of Lcy02g003010 vs. ExPASy TrEMBL
Match: A0A5A7TQJ9 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001150 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.9e-163
Identity = 274/315 (86.98%), Postives = 294/315 (93.33%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M+KI+HSTI+TNGINIH ASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSDAPPSPSSYTPHHI+GDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P  SPL+  R++LG+DFYICKFQEPGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKT-LPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTG 240
           DP P IIPNGFKT L TPE LP WLTEEDI+YFASKF+KTGFTGGFNYYRA+D+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWS ++IKVPTKFIVGDLDLVY+FPGAKEYIH GGFK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEISFLIYDFIKKF 315
            DEIS LIYDFI KF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of Lcy02g003010 vs. ExPASy TrEMBL
Match: A0A1S3BZ44 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.9e-163
Identity = 274/315 (86.98%), Postives = 294/315 (93.33%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M+KI+HSTI+TNGINIH ASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSDAPPSPSSYTPHHI+GDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P  SPL+  R++LG+DFYICKFQEPGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKT-LPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTG 240
           DP P IIPNGFKT L TPE LP WLTEEDI+YFASKF+KTGFTGGFNYYRA+D+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWS ++IKVPTKFIVGDLDLVY+FPGAKEYIH GGFK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEISFLIYDFIKKF 315
            DEIS LIYDFI KF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of Lcy02g003010 vs. NCBI nr
Match: XP_022984035.1 (uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 278/314 (88.54%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           ME+IEHSTIATNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P +SPLS  RQFLG+DFYICKFQ+PGVAEADFGS  TATMMKKFLT+R
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 524

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 525 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 584

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTKF+VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 585 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 644

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 645 DEISAHIYDFINKF 658

BLAST of Lcy02g003010 vs. NCBI nr
Match: XP_022984036.1 (uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037.1 uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 278/314 (88.54%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           ME+IEHSTIATNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P +SPLS  RQFLG+DFYICKFQ+PGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTKF+VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 301 DEISAHIYDFINKF 314

BLAST of Lcy02g003010 vs. NCBI nr
Match: XP_022934351.1 (uncharacterized protein LOC111441542 [Cucurbita moschata])

HSP 1 Score: 592.0 bits (1525), Expect = 2.9e-165
Identity = 277/314 (88.22%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           MEKIEHSTIATNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGI+QVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+ PR P +SPLS  RQFLG+DFYICKFQ PGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPETLP WLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTK++VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 301 DEISAHIYDFIIKF 314

BLAST of Lcy02g003010 vs. NCBI nr
Match: XP_038905064.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 276/314 (87.90%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M KI+HSTI TNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD  PSPSSYTPHHI+GDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+TPR P +SPL+ +R  LG++FY CKFQEPGVAEADFGS  TATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DPSPPIIPNGFKTL TPETLPPWLTEEDIDYFASKF KTGFTGGFNYYRA DL+WELTGP
Sbjct: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           W+G++IKVPTKFIVGDLD+VYH PGAKEYIH GGFK+ VPLLEEVVVIEGAAHFINQEKA
Sbjct: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300

Query: 301 DEISFLIYDFIKKF 315
            +IS LIYDFIKKF
Sbjct: 301 YDISSLIYDFIKKF 314

BLAST of Lcy02g003010 vs. NCBI nr
Match: KAG6581275.1 (hypothetical protein SDJN03_21277, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 275/314 (87.58%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           ME+IEHSTIATNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 19  MEQIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 78

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           GFGDSD PPSPSSYTP HIIGDLIG+LDHLGIDQVFLVGHDWGAMMAWYFCLFR DRVKA
Sbjct: 79  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 138

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVNLSVH+ PR P +SPLS  RQFLG+DFYICKFQ+PGVAEADFGS  TATMMKKFLT+R
Sbjct: 139 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 198

Query: 181 DPSPPIIPNGFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELTGP 240
           DP+PPIIP+GFKTL TPETLP WLTEEDIDYFASKF KTGFTGGFNYYRAI+ TWELTGP
Sbjct: 199 DPNPPIIPDGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 258

Query: 241 WSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQEKA 300
           WSG+KIKVPTK++VGD+D+VYH PGAK+YIH GGFK+DVPLLEEVVV+EGAAHFINQEKA
Sbjct: 259 WSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 318

Query: 301 DEISFLIYDFIKKF 315
           DEIS  IYDFI KF
Sbjct: 319 DEISAHIYDFIIKF 332

BLAST of Lcy02g003010 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 450.3 bits (1157), Expect = 1.3e-126
Identity = 199/316 (62.97%), Postives = 250/316 (79.11%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           MEKIEH+TI+TNGIN+HVASIGSGP +LF+HGFP+LWYSWRHQL+  A+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQVFLVGHDWGAMMAWYFCLFRSDRVKA 120
           G+GDSDAPPS  SYT  HI+GDL+G+LD LG+D+VFLVGHDWGA++AW+ C+ R DRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFLTLR 180
           LVN SV F PR P + P+ A R   G+D+YIC+FQEPG  E DF    T  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPSPPIIPN--GFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAIDLTWELT 240
           +P PP IP   GF+ LP P +LP WLTE+D+ ++  KF++ GFTGG NYYRA++L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 GPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAAHFINQE 300
            PW+G +IKVP KFIVGDLD+ Y+ PG KEYIH+GG K+ VP L+EVVV+EG  HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 KADEISFLIYDFIKKF 315
           K DE++  IY F KKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Lcy02g003010 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 379.4 bits (973), Expect = 2.8e-105
Identity = 191/323 (59.13%), Postives = 229/323 (70.90%), Query Frame = 0

Query: 4   IEHSTIATNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRG 63
           +EH  +  NGI+IHVA  G   GP VL LHGFPELWYSWRHQ+  LA++G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  FGDSDAPPSPSSYTPHHIIGDLIGVLDHLGI---DQVFLVGHDWGAMMAWYFCLFRSDRV 123
           +GDSDAP   SSYT  +I+GDLI V+  L     ++VF+VGHDWGA++AWY CLFR DRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVHFT--PRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKF 183
           KALVNLSV F+  P  P + P+  +R F G+D+YIC+FQE G  EA+     T  +MK+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTLRDPSPPIIPN-----GFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAI 243
           LT R P P IIP      G K    P  LP WLTEED+ YF SKF + GF+G  NYYR  
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNF 240

Query: 244 DLTWELTGPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGA 303
           +   EL GPW GSKI+VPTKF++G+LDLVY+ PG KEYIH   FKEDVPLLEE VV+EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 AHFINQEKADEISFLIYDFIKKF 315
           AHFINQEK  EI  +I DFI KF
Sbjct: 301 AHFINQEKPQEILQIILDFISKF 321

BLAST of Lcy02g003010 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.4e-101
Identity = 183/322 (56.83%), Postives = 224/322 (69.57%), Query Frame = 0

Query: 4   IEHSTIATNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRG 63
           +EH  +  NGI+IHVA  G   G  VL LHGFPELWYSWRHQ+  LA++G+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  FGDSDAPPSPSSYTPHHIIGDLIGVLDHLGID--QVFLVGHDWGAMMAWYFCLFRSDRVK 123
           +GDSDAP   SS+T  +I+GDL+ V+  L  +  +VF+VGHDWGA++AWY CLFR D+VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--HFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKFL 183
           ALVNLSV   F P  P + P+  +R   GND+Y+C+FQE G  EA+     T  +MK+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TLRDPSPPIIPN-----GFKTLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRAID 243
           T R P P IIP      G K    P  LP WLTEED+ YF SKF + GF G  NYYR  +
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFN 240

Query: 244 LTWELTGPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEGAA 303
              EL GPW GSKI+VPTKF++G+LDLVY+ PG KEYIH   FKEDVPL+EE VV+EG A
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFINQEKADEISFLIYDFIKKF 315
           HF+NQEK  EI  +I DFI  F
Sbjct: 301 HFLNQEKPQEILQIILDFISTF 320

BLAST of Lcy02g003010 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.6e-100
Identity = 166/323 (51.39%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPD 60
           ME I+H  ++ NGI +H+A  G   GP VL LHGFP+LWY+WRHQ+  L+S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGFGDSDAPPSPSSYTPHHIIGDLIGVLDHLGIDQ--VFLVGHDWGAMMAWYFCLFRSD 120
           LRG+GDSD+P S S YT  +++GDL+ +LD +  +Q  VFLVGHDWGA++ W+ CLFR +
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMMKKF 180
           ++   V LSV +  R P++ P+   +   G+D+YIC+FQEPG  E +  S      ++  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTLRDPSPPIIP--NGFKTLPTPET----LPPWLTEEDIDYFASKFAKTGFTGGFNYYRA 240
            T R   PPI+P  N F   P P +    LP W +++D+D++ SKF K GFTGG NYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 IDLTWELTGPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEG 300
           +DL WELT PW+G+KI+VP KF+ GD D+VY  PG KEYIH GGF  DVP L+E+VVIE 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AAHFINQEKADEISFLIYDFIKK 314
           A HF+NQEK  E++  I DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Lcy02g003010 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.3e-98
Identity = 164/324 (50.62%), Postives = 225/324 (69.44%), Query Frame = 0

Query: 1   MEKIEHSTIATNGINIHVA-----SIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAI 60
           ++ +EH T+  NGIN+HVA       G  P +LFLHGFPELWY+WRHQ++ L+S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGFGDSDAPPSPSSYTPHHIIGDLIGVLDHL--GIDQVFLVGHDWGAMMAWYFCLF 120
           APDLRG+GD++AP     YT  ++ GD++ ++D +  G   V +VGHDWGAM+AW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RSDRVKALVNLSVHFTPRFPEMSPLSALRQFLGNDFYICKFQEPGVAEADFGSDSTATMM 180
           R ++VKALVN+SV F+PR P   P+  LR   G+D+Y+C+FQ+ G  E +F    T  ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTLRDPSPPIIPNG--FK-TLPTPETLPPWLTEEDIDYFASKFAKTGFTGGFNYYRA 240
           K+FLT + P P  +P    FK +      LP WLT+ED+DY+ +K+   GFTG  NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 IDLTWELTGPWSGSKIKVPTKFIVGDLDLVYHFPGAKEYIHDGGFKEDVPLLEEVVVIEG 300
           ID  WELT PW+G+KI+VP KFI+GD DL Y+FPGAKEYI+ GGFK DVPLL+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKADEISFLIYDFIKKF 315
             HF+++E  D I+  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS01.6e-6039.62Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349131.8e-4833.88Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349148.9e-4834.20Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Q6Q2C28.9e-4837.50Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802997.5e-4733.33Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1J1077.5e-16788.54uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J7K47.5e-16788.54uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1F1K31.4e-16588.22uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A5A7TQJ93.9e-16386.98Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZ443.9e-16386.98bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE... [more]
Match NameE-valueIdentityDescription
XP_022984035.11.6e-16688.54uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima][more]
XP_022984036.11.6e-16688.54uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037... [more]
XP_022934351.12.9e-16588.22uncharacterized protein LOC111441542 [Cucurbita moschata][more]
XP_038905064.15.0e-16587.90epoxide hydrolase A-like [Benincasa hispida][more]
KAG6581275.11.5e-16487.58hypothetical protein SDJN03_21277, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT4G02340.11.3e-12662.97alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.12.8e-10559.13soluble epoxide hydrolase [more]
AT2G26750.12.4e-10156.83alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.11.6e-10051.39alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.13.3e-9850.62alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 51..66
score: 51.51
coord: 97..110
score: 31.03
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..147
e-value: 2.4E-24
score: 86.4
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 111..124
score: 49.35
coord: 31..49
score: 56.94
coord: 248..264
score: 44.39
coord: 288..310
score: 39.53
coord: 97..110
score: 48.7
coord: 51..66
score: 56.82
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..314
e-value: 2.1E-108
score: 364.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..313
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..314
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g003010.1Lcy02g003010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity