Lcy02g002250 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g002250
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr02: 3125543 .. 3127504 (-)
RNA-Seq Expression	Lcy02g002250
Synteny	Lcy02g002250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAAAAATAAAAATAAAAATACAAAAATATTTCAATAAAATAAAATAAAAAAAGAGAAAAAGTCTTCTTCTTCCTTATAGAGTGATCAAAAGAGAACTGACTTTGCCATTCTCGATAACTGCAGAAGAAGAAGGAGGAGGAGAAGATGGCGGATTCTCGTGCAGCGAACAACGAAAAGGAGAGGCGGCGTTCGGTCAGCAGTAGCAGAGGGAAGAGCGACTCGATGAACGTCATATTCACTGATCCCCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTTTCAGGTCCGATCAAGCTTTTTTTTTGCTTTAAATTGTTGTTATTGTTCATCTGGATGTATATGATGCTTTATTCTTCTCGTTCGTTTTCGTTTTTTTTGTGATTTGAGTACTATAGTCACTTGTGTCTGTTTCTTGGATTTGACGATTTTGAAGTCCCATTTCTGTGAGCTTTTTTATCTTCTTTTTTGGAAGTTTATTGTGTAAACTGTGGATTTCTGATAAAATTTCAAGAAATATGCGAAGTTATTTACTGTTAGCTGAAGATTGTGTTGCCAAAGTACTAGCAAAATGGAGATTATTCTGGATATTGTGGGGACTGGCTCGAGAAATGGTTGGAATTGAAATGAAATATTGACCTGAATTGCTTATCTTGTTATATTATCTCGAGTGAACGTCAAAATCAGAATCAAGCCATGTTTATCTGACATTTTCTTCACCTTTATATGCCAAACTTGGGGTTCAATCGTCTGGATTATGGGATTTTCTTCATTGTTTAGAAACATGAATTTGAAAGGGATGAATATAGAAGAAAATTAAGCTTGCATGTGTGTATACAATATCTTATTTTGTTATTTATGAATCTATTTTATTCCATAACATATACATTGATCTTGGGCTGTTTGTGTTGATGAATTAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGGCAATTGGCTACATACGTTGCAGAGCGATCGAGAAAGTTCTGGAATCAATCAAGATACCCTGCCAAAACTCGATGTACGGATGCAAATTGACAATGAAGTTGAACCAGATAAATGACCATGAGTCATCGTGCACATACGAACCCTGCTCATGCCCTTTATCTGATTGCTTATATGTTGGTTCTTCTGAGAAGCTGTGCTCACACTTCAGAAAAAAGCACAAAGACTCAGCCAAAAGCTTCTTATACAATTCGAGGTTCACTATATGTTTGAACAGTGGTGATAGTTACCGTATCCTTCAAGCAGAGAATGATGGAGTTTTGTTCTTCCTCTCTTACACGTTTGAAATTTTCGGGAATGCGGTAACCGTGAATCGGATTGGACCTTCATCGTCTGAGAGAAAGTATTGTTATGAAATTAAAGCGAAAACGCAGGGGAGTATTCTTTCTTTGCAATCAGTAGCGAAAGAAATGCAAGCTCGCACTGAAGTTCCTCCTTCCAAGGGATCGCTTTTGATTCCGAACGAGTACTTTGGATCTTCTGCCCAGACGATCTTGGAGTTTCGCATTTGGCCTGCACTCTAAAGACAAAGACTGGGGATCGACACAGGTGCACATACTTTTGCAAGAAAGCTGATTATTTATAAACTGAGTTAAAGATTGTTGGCTGGCTGTGATCTTGATTGCCTAATCTTTGCATCTGTGTGTGTGTGTGTGTTTTTTTTAAGGAAAGGATTTAGTGCCTACTTTTGTACATTTCATCTTTTGTCTTAGATATCTAGAAATAGGAAGTATTGCATTTTGTATTGAGGGAAGAAGTATGCTCAAGAAATAGGAGGGGGGGTTTGGTATGCACTATGTAGATACATGGAATTGTGCTTCATAATCTCATCCTTCTATAACTTTGATCCATTCTCAGGTTTCACATTCTTGAGTTTGATAAGTTATATCATTAT

mRNA sequence

AAGAAAAAATAAAAATAAAAATACAAAAATATTTCAATAAAATAAAATAAAAAAAGAGAAAAAGTCTTCTTCTTCCTTATAGAGTGATCAAAAGAGAACTGACTTTGCCATTCTCGATAACTGCAGAAGAAGAAGGAGGAGGAGAAGATGGCGGATTCTCGTGCAGCGAACAACGAAAAGGAGAGGCGGCGTTCGGTCAGCAGTAGCAGAGGGAAGAGCGACTCGATGAACGTCATATTCACTGATCCCCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTTTCAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGGCAATTGGCTACATACGTTGCAGAGCGATCGAGAAAGTTCTGGAATCAATCAAGATACCCTGCCAAAACTCGATGTACGGATGCAAATTGACAATGAAGTTGAACCAGATAAATGACCATGAGTCATCGTGCACATACGAACCCTGCTCATGCCCTTTATCTGATTGCTTATATGTTGGTTCTTCTGAGAAGCTGTGCTCACACTTCAGAAAAAAGCACAAAGACTCAGCCAAAAGCTTCTTATACAATTCGAGGTTCACTATATGTTTGAACAGTGGTGATAGTTACCGTATCCTTCAAGCAGAGAATGATGGAGTTTTGTTCTTCCTCTCTTACACGTTTGAAATTTTCGGGAATGCGGTAACCGTGAATCGGATTGGACCTTCATCGTCTGAGAGAAAGTATTGTTATGAAATTAAAGCGAAAACGCAGGGGAGTATTCTTTCTTTGCAATCAGTAGCGAAAGAAATGCAAGCTCGCACTGAAGTTCCTCCTTCCAAGGGATCGCTTTTGATTCCGAACGAGTACTTTGGATCTTCTGCCCAGACGATCTTGGAGTTTCGCATTTGGCCTGCACTCTAAAGACAAAGACTGGGGATCGACACAGGTGCACATACTTTTGCAAGAAAGCTGATTATTTATAAACTGAGTTAAAGATTGTTGGCTGGCTGTGATCTTGATTGCCTAATCTTTGCATCTGTGTGTGTGTGTGTGTTTTTTTTAAGGAAAGGATTTAGTGCCTACTTTTGTACATTTCATCTTTTGTCTTAGATATCTAGAAATAGGAAGTATTGCATTTTGTATTGAGGGAAGAAGTATGCTCAAGAAATAGGAGGGGGGGTTTGGTATGCACTATGTAGATACATGGAATTGTGCTTCATAATCTCATCCTTCTATAACTTTGATCCATTCTCAGGTTTCACATTCTTGAGTTTGATAAGTTATATCATTAT

Coding sequence (CDS)

ATGGCGGATTCTCGTGCAGCGAACAACGAAAAGGAGAGGCGGCGTTCGGTCAGCAGTAGCAGAGGGAAGAGCGACTCGATGAACGTCATATTCACTGATCCCCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTTTCAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGGCAATTGGCTACATACGTTGCAGAGCGATCGAGAAAGTTCTGGAATCAATCAAGATACCCTGCCAAAACTCGATGTACGGATGCAAATTGACAATGAAGTTGAACCAGATAAATGACCATGAGTCATCGTGCACATACGAACCCTGCTCATGCCCTTTATCTGATTGCTTATATGTTGGTTCTTCTGAGAAGCTGTGCTCACACTTCAGAAAAAAGCACAAAGACTCAGCCAAAAGCTTCTTATACAATTCGAGGTTCACTATATGTTTGAACAGTGGTGATAGTTACCGTATCCTTCAAGCAGAGAATGATGGAGTTTTGTTCTTCCTCTCTTACACGTTTGAAATTTTCGGGAATGCGGTAACCGTGAATCGGATTGGACCTTCATCGTCTGAGAGAAAGTATTGTTATGAAATTAAAGCGAAAACGCAGGGGAGTATTCTTTCTTTGCAATCAGTAGCGAAAGAAATGCAAGCTCGCACTGAAGTTCCTCCTTCCAAGGGATCGCTTTTGATTCCGAACGAGTACTTTGGATCTTCTGCCCAGACGATCTTGGAGTTTCGCATTTGGCCTGCACTCTAA

Protein sequence

MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVPPSKGSLLIPNEYFGSSAQTILEFRIWPAL

Homology

[Close]

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.2e-53
Identity = 105/252 (41.67%), Postives = 148/252 (58.73%), Query Frame = 0


Query: 16  SVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQNKCPSCS 75
           S SSS  K   ++V   DP +LDC ICCEPL +P+FQC+NGH++C+ CCTKV+N+CPSC+
Sbjct: 90  SQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCT 149

Query: 76  LAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPLSDCLYV 135
           L IGY+RCRA+EKV+E+ ++ C N+ YGCK +        HE  C + PCSCP+ DC Y 
Sbjct: 150 LPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYT 209

Query: 136 GSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLSYTFEIFGN 195
           G  + L +H R +HKD   SF++N+R TI L+      ILQ ENDG +  +     +   
Sbjct: 210 GYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAV 269

Query: 196 AVTVNRIGP-SSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVPPSKGSLLIPNEYFG 255
            V+V+ I P +    +    +   T  S+L    + K +Q  T   P  G +LIP+  F 
Sbjct: 270 YVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLF- 329

Query: 256 SSAQTILEFRIW 267
            S    L  +IW
Sbjct: 330 -SGNDNLNLQIW 339

[Close]

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.8e-39
Identity = 93/260 (35.77%), Postives = 138/260 (53.08%), Query Frame = 0


Query: 12  ERRRSVSSSRGKSDS--MNVIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQN 71
           ++R+  SS   K D+   + +  D +ILDC IC E  ++P+FQC+NGH++CSSCC K+ N
Sbjct: 22  QKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNN 81

Query: 72  KCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPL 131
           KCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  +   +   HE  C +  C+CP 
Sbjct: 82  KCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPA 141

Query: 132 SDCLYVGSSEKLCSHFRKKHKD--SAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLS 191
            DC Y  S + L +H+R  H +     +F+ +   ++ +N      I        LF + 
Sbjct: 142 LDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQ 201

Query: 192 YTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKTQGSILSLQS-VAKEMQARTEVPPSKGS 251
              E +G  VTV+ I PSS E  +Y Y +     G  +  QS   K +   +   P +  
Sbjct: 202 CFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENF 261

Query: 252 LLIPNEYFGSSAQTILEFRI 266
           +LIPN         +LE RI
Sbjct: 262 MLIPNSLLRGD---VLEMRI 278

[Close]

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-37
Identity = 90/255 (35.29%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 4   SRAANNEKERRRSVSSSRGK---SDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 63
           S    ++K    S+SS  G+     + + + TD  ILDC IC + L +PVFQC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 123
           SSCC K++NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYN--SRFTICLNSGDSYRILQAE 183
            + PCSCP+  C Y GS + L  H+   H   + ++ +N  S     +   D   I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDGVLFFLSYTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKT-QGSILSLQS-VAKEMQA 243
              +LF +    E  G  V+V+ I PS+ E  ++ Y +   T +G  ++ QS   K++  
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 RTEVPPSKGSLLIPN 251
            +   P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

[Close]

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.1e-37
Identity = 82/228 (35.96%), Postives = 125/228 (54.82%), Query Frame = 0


Query: 29  VIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQNKCPSCSLAIGYIRCRAIEK 88
           ++  D  ILDC ICCE L+ P+FQC+NGH++C SCC K+ NKCP+C+L +G+ R RA+E 
Sbjct: 28  IVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMES 87

Query: 89  VLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPLSDCLYVGSSEKLCSHFRKK 148
           VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y GS + L +H++  
Sbjct: 88  VLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLT 147

Query: 149 HKD----SAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLSYTFEIFGNAVTVNRIGP 208
           H      + K F   + FT  +   D   I +     +L  +    E  G  VTV+ I P
Sbjct: 148 HSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAP 207

Query: 209 SSSE-RKYCYEIKAKTQGSILSLQSVAKEMQARTEV-PPSKGSLLIPN 251
           S+ E  ++ Y++     G  ++ +S   +   +  +  P +  +LIP+
Sbjct: 208 SAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPH 255

[Close]

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot
Match: Q9FM14 (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 5.1e-36
Identity = 89/269 (33.09%), Postives = 140/269 (52.04%), Query Frame = 0


Query: 1   MADSRA-ANNEKERRRS---VSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENG 60
           M DS +   N+  +R+S       R ++++ +    D  +LDC +C EPL++P FQC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCTKVQNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQIN 120
           HI C+ C  KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  ++   +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 DHESSCTYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C Y GS   +  HF ++H       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDSYRILQAENDGVLFFLSYTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKTQGSI 240
            +N  +   +L      +LF +    E  G  VTV RI P +SE +K+ Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQS--VAKEMQARTEVPPSKGSLLIPN 251
           ++ +S  V + ++  +++ P    + +PN
Sbjct: 241 VTYESPEVKRLLEVNSQI-PDDSFMFVPN 261

[Close]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.0e-127
Identity = 221/268 (82.46%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSRA++ E+ +R  V S RGK+++MNVIF+DPQ+LDCYICCEPLS PVFQCENGHI+C
Sbjct: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCCTKVQNKCPSCSL+IGYIRCRAIEKVLESIKIPC NS+YGCK+TM LN+ NDH S C
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
           TYEPCSCP+SDCL+VGSSEKLCSHF KKHK+SAKSF YNSRFT+CLNSGDSYRILQAE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
           G LFFLS+TFEIFGNAVTV+RIGPSSSE+K+CYEIKAKTQGSIL LQS+AKE+Q R EVP
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PSKGSLLIPNEYFGSSAQTILEFRIWPA 269
           PS  SLLIPNE+FGSSAQTILEFRIWP+
Sbjct: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPS 267

[Close]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 217/269 (80.67%), Postives = 244/269 (90.71%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSR+++ EKERRRSV+ SRGK+++M VIFTDPQILDCYICCEPLSVPV+QCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCK+TM LNQIN+HE+SC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCP+SDC YV S+E L SHFRK+H+DSAKSFLYNSRFTICLNSGD YRI QAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
             LF LSY+FEIFGNAVT++RIG   SE+K+CY+IKAK+QGS+LSLQSVAKE+Q  TE P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           P KGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 9.1e-121
Identity = 212/269 (78.81%), Postives = 240/269 (89.22%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSR+++ EKERRRSV+ SRGK+++M VIFTDPQILDCYICCEPLSVPV+QCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCK+TM LNQIN+HE+SC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCP+SDC YV S+E L SHFRK+H DSAKSFLYNSRFTICLNSGD YRI QAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
             +F LSY+FEIFGNAVT++RIG    E+K+CY+IKAK+QGS+LSLQSVA E++   E P
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           P KGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL
Match: A0A0A0L9Z6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.6e-120
Identity = 208/268 (77.61%), Postives = 237/268 (88.43%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           M DSR+++   ERR SV S RG + ++NVIFTDPQILDCYICCEPLS+PVFQCENGHI+C
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCCTK QNKCPSC+LAIGYIRCRAIEKVLESIK+PCQN++YGCK  M LN INDHES C
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCPL +C +VGS+E+L  HF KKHKDSAK F YN+RFTICLN+GD++RIL+AEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
           GVLFFLSYTFEIFGNAVT+NRIGP SSE+K+CYEIKAKT GS+LSLQS+AKE+Q   +VP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEYFGSSAQTILEFRIWPA 269
           PSKGSLLIPNEYFGSS QT+LE  IWPA
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPA 268

[Close]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.3e-114
Identity = 203/269 (75.46%), Postives = 233/269 (86.62%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MA SR+++ EKERR SV+SSR K+++M VI TDPQ+LDCYICC PLSVPV+QCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSL IGY+RCRAIEKVLESIK+PCQNS YGCK T  LNQIN+HE+ C
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            Y+PCSCPLSDC YV S+E L SHFRK+H+DSAKS  YNSRFTICLNS D Y I QAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
           GVLF LSY+FE FG+AVT+ RIGP SSE+K+CY+IKAK+ G++LSL SVAKE+Q  TE P
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           PSKGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 466.1 bits (1198), Expect = 2.1e-127
Identity = 221/268 (82.46%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSRA++ E+ +R  V S RGK+++MNVIF+DPQ+LDCYICCEPLS PVFQCENGHI+C
Sbjct: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCCTKVQNKCPSCSL+IGYIRCRAIEKVLESIKIPC NS+YGCK+TM LN+ NDH S C
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
           TYEPCSCP+SDCL+VGSSEKLCSHF KKHK+SAKSF YNSRFT+CLNSGDSYRILQAE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
           G LFFLS+TFEIFGNAVTV+RIGPSSSE+K+CYEIKAKTQGSIL LQS+AKE+Q R EVP
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PSKGSLLIPNEYFGSSAQTILEFRIWPA 269
           PS  SLLIPNE+FGSSAQTILEFRIWP+
Sbjct: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPS 267

[Close]

BLAST of Lcy02g002250 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 452.6 bits (1163), Expect = 2.4e-123
Identity = 217/269 (80.67%), Postives = 244/269 (90.71%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSR+++ EKERRRSV+ SRGK+++M VIFTDPQILDCYICCEPLSVPV+QCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCK+TM LNQIN+HE+SC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCP+SDC YV S+E L SHFRK+H+DSAKSFLYNSRFTICLNSGD YRI QAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
             LF LSY+FEIFGNAVT++RIG   SE+K+CY+IKAK+QGS+LSLQSVAKE+Q  TE P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           P KGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. NCBI nr
Match: KAG6606996.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 444.9 bits (1143), Expect = 5.0e-121
Identity = 213/269 (79.18%), Postives = 241/269 (89.59%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSR+++ EKERRRSV+ SRGK+++M VIFTDPQILDCYICCEPLSVPV+QCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCK+TM LNQIN+HE+SC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCP+SDC YV S+E L SHFRK+H+DSAKSFLYNSRFTICLNSGD YRI QAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
             LF LSY+FEIFGNAVT++RIG    E+K+CY+IKAK+QGS+LSLQSVA E++   E P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           P KGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. NCBI nr
Match: XP_022949045.1 (uncharacterized protein LOC111452508 [Cucurbita moschata])

HSP 1 Score: 443.0 bits (1138), Expect = 1.9e-120
Identity = 212/269 (78.81%), Postives = 240/269 (89.22%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           MADSR+++ EKERRRSV+ SRGK+++M VIFTDPQILDCYICCEPLSVPV+QCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCC+KV NKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCK+TM LNQIN+HE+SC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCP+SDC YV S+E L SHFRK+H DSAKSFLYNSRFTICLNSGD YRI QAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
             +F LSY+FEIFGNAVT++RIG    E+K+CY+IKAK+QGS+LSLQSVA E++   E P
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PSKGSLLIPNEYFG-SSAQTILEFRIWPA 269
           P KGSLLIPNE+FG SSAQTILE RIWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Lcy02g002250 vs. NCBI nr
Match: XP_004140738.2 (E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypothetical protein Csa_011184 [Cucumis sativus])

HSP 1 Score: 442.2 bits (1136), Expect = 3.2e-120
Identity = 208/268 (77.61%), Postives = 237/268 (88.43%), Query Frame = 0


Query: 1   MADSRAANNEKERRRSVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 60
           M DSR+++   ERR SV S RG + ++NVIFTDPQILDCYICCEPLS+PVFQCENGHI+C
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 120
           SSCCTK QNKCPSC+LAIGYIRCRAIEKVLESIK+PCQN++YGCK  M LN INDHES C
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAEND 180
            YEPCSCPL +C +VGS+E+L  HF KKHKDSAK F YN+RFTICLN+GD++RIL+AEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTVNRIGPSSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVP 240
           GVLFFLSYTFEIFGNAVT+NRIGP SSE+K+CYEIKAKT GS+LSLQS+AKE+Q   +VP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEYFGSSAQTILEFRIWPA 269
           PSKGSLLIPNEYFGSS QT+LE  IWPA
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPA 268

[Close]

BLAST of Lcy02g002250 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 211.5 bits (537), Expect = 8.6e-55
Identity = 105/252 (41.67%), Postives = 148/252 (58.73%), Query Frame = 0


Query: 16  SVSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQNKCPSCS 75
           S SSS  K   ++V   DP +LDC ICCEPL +P+FQC+NGH++C+ CCTKV+N+CPSC+
Sbjct: 90  SQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCT 149

Query: 76  LAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPLSDCLYV 135
           L IGY+RCRA+EKV+E+ ++ C N+ YGCK +        HE  C + PCSCP+ DC Y 
Sbjct: 150 LPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYT 209

Query: 136 GSSEKLCSHFRKKHKDSAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLSYTFEIFGN 195
           G  + L +H R +HKD   SF++N+R TI L+      ILQ ENDG +  +     +   
Sbjct: 210 GYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAV 269

Query: 196 AVTVNRIGP-SSSERKYCYEIKAKTQGSILSLQSVAKEMQARTEVPPSKGSLLIPNEYFG 255
            V+V+ I P +    +    +   T  S+L    + K +Q  T   P  G +LIP+  F 
Sbjct: 270 YVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLF- 329

Query: 256 SSAQTILEFRIW 267
            S    L  +IW
Sbjct: 330 -SGNDNLNLQIW 339

[Close]

BLAST of Lcy02g002250 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 163.3 bits (412), Expect = 2.7e-40
Identity = 93/260 (35.77%), Postives = 138/260 (53.08%), Query Frame = 0


Query: 12  ERRRSVSSSRGKSDS--MNVIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQN 71
           ++R+  SS   K D+   + +  D +ILDC IC E  ++P+FQC+NGH++CSSCC K+ N
Sbjct: 22  QKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNN 81

Query: 72  KCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPL 131
           KCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  +   +   HE  C +  C+CP 
Sbjct: 82  KCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPA 141

Query: 132 SDCLYVGSSEKLCSHFRKKHKD--SAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLS 191
            DC Y  S + L +H+R  H +     +F+ +   ++ +N      I        LF + 
Sbjct: 142 LDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQ 201

Query: 192 YTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKTQGSILSLQS-VAKEMQARTEVPPSKGS 251
              E +G  VTV+ I PSS E  +Y Y +     G  +  QS   K +   +   P +  
Sbjct: 202 CFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENF 261

Query: 252 LLIPNEYFGSSAQTILEFRI 266
           +LIPN         +LE RI
Sbjct: 262 MLIPNSLLRGD---VLEMRI 278

[Close]

BLAST of Lcy02g002250 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 158.3 bits (399), Expect = 8.6e-39
Identity = 90/255 (35.29%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 4   SRAANNEKERRRSVSSSRGK---SDSMNVIFTDPQILDCYICCEPLSVPVFQCENGHISC 63
           S    ++K    S+SS  G+     + + + TD  ILDC IC + L +PVFQC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCTKVQNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQINDHESSC 123
           SSCC K++NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 TYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYN--SRFTICLNSGDSYRILQAE 183
            + PCSCP+  C Y GS + L  H+   H   + ++ +N  S     +   D   I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDGVLFFLSYTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKT-QGSILSLQS-VAKEMQA 243
              +LF +    E  G  V+V+ I PS+ E  ++ Y +   T +G  ++ QS   K++  
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 RTEVPPSKGSLLIPN 251
            +   P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

[Close]

BLAST of Lcy02g002250 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 157.5 bits (397), Expect = 1.5e-38
Identity = 82/228 (35.96%), Postives = 125/228 (54.82%), Query Frame = 0


Query: 29  VIFTDPQILDCYICCEPLSVPVFQCENGHISCSSCCTKVQNKCPSCSLAIGYIRCRAIEK 88
           ++  D  ILDC ICCE L+ P+FQC+NGH++C SCC K+ NKCP+C+L +G+ R RA+E 
Sbjct: 28  IVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMES 87

Query: 89  VLESIKIPCQNSMYGCKLTMKLNQINDHESSCTYEPCSCPLSDCLYVGSSEKLCSHFRKK 148
           VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y GS + L +H++  
Sbjct: 88  VLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLT 147

Query: 149 HKD----SAKSFLYNSRFTICLNSGDSYRILQAENDGVLFFLSYTFEIFGNAVTVNRIGP 208
           H      + K F   + FT  +   D   I +     +L  +    E  G  VTV+ I P
Sbjct: 148 HSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAP 207

Query: 209 SSSE-RKYCYEIKAKTQGSILSLQSVAKEMQARTEV-PPSKGSLLIPN 251
           S+ E  ++ Y++     G  ++ +S   +   +  +  P +  +LIP+
Sbjct: 208 SAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPH 255

[Close]

BLAST of Lcy02g002250 vs. TAIR 10
Match: AT5G62800.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 152.9 bits (385), Expect = 3.6e-37
Identity = 89/269 (33.09%), Postives = 140/269 (52.04%), Query Frame = 0


Query: 1   MADSRA-ANNEKERRRS---VSSSRGKSDSMNVIFTDPQILDCYICCEPLSVPVFQCENG 60
           M DS +   N+  +R+S       R ++++ +    D  +LDC +C EPL++P FQC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCTKVQNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSMYGCKLTMKLNQIN 120
           HI C+ C  KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  ++   +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 DHESSCTYEPCSCPLSDCLYVGSSEKLCSHFRKKHKDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C Y GS   +  HF ++H       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDSYRILQAENDGVLFFLSYTFEIFGNAVTVNRIGPSSSE-RKYCYEIKAKTQGSI 240
            +N  +   +L      +LF +    E  G  VTV RI P +SE +K+ Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQS--VAKEMQARTEVPPSKGSLLIPN 251
           ++ +S  V + ++  +++ P    + +PN
Sbjct: 241 VTYESPEVKRLLEVNSQI-PDDSFMFVPN 261

The following BLAST results are available for this feature:

BLAST of Lcy02g002250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K34	1.2e-53	41.67	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD7	3.8e-39	35.77	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]
Q9FKD9	1.2e-37	35.29	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FKD5	2.1e-37	35.96	Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FM14	5.1e-36	33.09	E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]

BLAST of Lcy02g002250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNI1	1.0e-127	82.46	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1K8G5	1.2e-123	80.67	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P...	[more]
A0A6J1GBP1	9.1e-121	78.81	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508...	[more]
A0A0A0L9Z6	1.6e-120	77.61	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE...	[more]
A0A6J1KAW2	1.3e-114	75.46	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]

BLAST of Lcy02g002250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143325.1	2.1e-127	82.46	E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia]	[more]
XP_022997791.1	2.4e-123	80.67	E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima]	[more]
KAG6606996.1	5.0e-121	79.18	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]
XP_022949045.1	1.9e-120	78.81	uncharacterized protein LOC111452508 [Cucurbita moschata]	[more]
XP_004140738.2	3.2e-120	77.61	E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypotheti...	[more]

BLAST of Lcy02g002250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37930.1	8.6e-55	41.67	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	2.7e-40	35.77	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	8.6e-39	35.29	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37910.1	1.5e-38	35.96	Protein with RING/U-box and TRAF-like domains	[more]
AT5G62800.1	3.6e-37	33.09	Protein with RING/U-box and TRAF-like domains	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 81..235 e-value: 6.6E-20 score: 71.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 87..145 e-value: 3.5E-16 score: 61.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 9..267
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 92..253
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 9..267
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 92..150 score: 13.724751

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g002250.1	Lcy02g002250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding