Lcy02g001710 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g001710 vs. ExPASy Swiss-Prot
Match: F4KE63 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 1586.2 bits (4106), Expect = 0.0e+00
Identity = 769/970 (79.28%), Postives = 866/970 (89.28%), Query Frame = 0

Query: 56   MLLQMAFSGP----SLLPPCSAYRLNPLILT-KQRRAFGLSRFHFRRPQLRL-LAVAASA 115
            M+L+ AFS P    +LL P S ++LN L  T ++RR    SR +    Q R   + AAS 
Sbjct: 1    MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRLISPSRLNSIFSQRRFSFSAAASG 60

Query: 116  NGVFTSPEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHA 175
            N VFTSPE +KTFDF+SEE+IY+WWESQGYFKP FDQ   PFVI MPPPNVTGSLHMGHA
Sbjct: 61   NNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGHA 120

Query: 176  MFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRV 235
            MFVTLEDIMVRYNRM GRPTLWLPGTDHAGIATQLVVE+MLASEGIKRV+LGRDEFTKRV
Sbjct: 121  MFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKRV 180

Query: 236  WEWKEKYGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVN 295
            WEWKEKYGGTITNQIKRLGASCDW+RE FTLD+QLSRAVVEAFV+LH++GLIYQGSYMVN
Sbjct: 181  WEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVN 240

Query: 296  WSPNLQTAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQ 355
            WSPNLQTAVSDLEVEYSEE G LYHIKYRVAG    D+LT+ATTRPETLFGDVA+AVHP+
Sbjct: 241  WSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAGS--PDFLTIATTRPETLFGDVALAVHPE 300

Query: 356  DERYSKYVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPI 415
            D+RYSKYVG  AIVPMTYGRHVPII+DK VDKDFGTGVLKISPGHDHNDYLLARKLGLPI
Sbjct: 301  DDRYSKYVGQTAIVPMTYGRHVPIIADKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPI 360

Query: 416  LNVMNKDGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIE 475
            LNVMNKD TLN VAGL+CGLDRFE R+KLWADLEE GLAVKKE HTLRVPRSQRGGE+IE
Sbjct: 361  LNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVIE 420

Query: 476  PLVSKQWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRI 535
            PLVSKQWFV M+PLAEKAL AVE  +LTIIPERFEKIYNHWL+NIKDWCISRQLWWGHRI
Sbjct: 421  PLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHRI 480

Query: 536  PVWYIVGKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGW 595
            PVWY+VGK+ EEDYIVA++A+EALE+A +KYGKDVEIYQDPDVLDTWFSS+LWPFSTLGW
Sbjct: 481  PVWYVVGKDCEEDYIVAKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLGW 540

Query: 596  PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 655
            PD  A+DF  FYPT MLETGHDILFFWVARMVM+GIEFTGTVPFS++YLHGLIRDSQGRK
Sbjct: 541  PDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRK 600

Query: 656  MSKTLGNVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFI 715
            MSK+LGNVIDPLDTIK+FGTDALRFT+ALGTAGQDLNLSTERLT+NKAFTNKLWNAGKF+
Sbjct: 601  MSKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLSTERLTANKAFTNKLWNAGKFV 660

Query: 716  LQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDV 775
            L +LPS +D+ +WE++   + +KE+ LL LPLPECW VS+LH LID VT SY+K FFGDV
Sbjct: 661  LHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGDV 720

Query: 776  GRDVYNFFWGDFADWYIEASKARLYQSGGDSVALA-QAVLLYVFENILKLLHPFMPFVTE 835
            GR+ Y+FFW DFADWYIEASK+RLY SGG+SV+LA QAVLLYVFENILKLLHPFMPFVTE
Sbjct: 721  GRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTE 780

Query: 836  ELWQALPHRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISA 895
            +LWQALP+RKEALI+SPWPQ SLP+   ++K+FENL+ LT+ IRNARAEYSVEP KRISA
Sbjct: 781  DLWQALPYRKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRISA 840

Query: 896  SIVASEEVNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLAD 955
            S+V S EV +YIS+EKEVLALL+RLDL+NV+F+N+PPG+ + SVHLVA EGLEAYLPLA 
Sbjct: 841  SVVGSAEVIEYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEAYLPLAA 900

Query: 956  MVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALT 1015
            MVDIS+E+QR+SKRL+KM+TEYD  I RL+SP FVEKAPE++VRGV+E+ EE +EKI LT
Sbjct: 901  MVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLT 960

Query: 1016 EKRLSLLRST 1019
            + RL  L+ST
Sbjct: 961  KARLDFLKST 968

BLAST of Lcy02g001710 vs. ExPASy Swiss-Prot
Match: Q8RBN5 (Valine--tRNA ligase OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=valS PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 1.4e-238
Identity = 410/906 (45.25%), Postives = 595/906 (65.67%), Query Frame = 0

Query: 117  EIAKTFDFTS-EERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 176
            +IAKT++    E+RIY +W  +G+F P+ D +  PF I +PPPNVTG LHMGHA+  TL+
Sbjct: 3    DIAKTYNPKEFEDRIYSFWMEKGFFTPEIDPEKKPFTIVIPPPNVTGELHMGHALDNTLQ 62

Query: 177  DIMVRYNRMKGRPTLWLPGTDHAGIATQL-VVERMLASEGIKRVELGRDEFTKRVWEWKE 236
            DI++R+ RM+G   LW+PGTDHA IAT++ V++++    G  + +L R+EF ++ WEWK+
Sbjct: 63   DILIRWRRMQGYAALWIPGTDHASIATEIKVLDKIREETGKTKKDLTREEFLEKAWEWKD 122

Query: 237  KYGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNL 296
            KY   I +Q+K+LG+SCDWTR  FT+D++ S+AV E FV L+E+GLIY+G+ ++NW P+ 
Sbjct: 123  KYENRILSQLKKLGSSCDWTRTAFTMDEKRSKAVREVFVSLYEKGLIYKGNRIINWCPSC 182

Query: 297  QTAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYS 356
             TA+SD EVE+ E  G L++IKY V G    DY+ +ATTRPET+ GDVA+AVHP+DERY 
Sbjct: 183  NTALSDAEVEHKEHKGHLWYIKYPVKG--EEDYVVIATTRPETMLGDVAVAVHPEDERYR 242

Query: 357  KYVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMN 416
              +G   I+P+  GR +P+I+D+ VD  FGTG +K++P HD ND+ +  +  LP +N+MN
Sbjct: 243  HLIGKTLILPLV-GREIPVIADEYVDPSFGTGAVKVTPAHDPNDFEIGVRHNLPFVNIMN 302

Query: 417  KDGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSK 476
            ++ T+N+  G Y GLDR+EAR+K+  DLEE GL +K E H   V    R   ++EPL+S+
Sbjct: 303  ENATINENGGKYEGLDRYEAREKIVKDLEEQGLLLKVEEHVHNVGHCYRCDTVVEPLLSE 362

Query: 477  QWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYI 536
            QWFV MEPLA+ AL+ V+ G +  +PERFEKIY +WL NIKDWCISRQLWWGHRIP WY 
Sbjct: 363  QWFVKMEPLAKPALQVVKEGKIKFVPERFEKIYTNWLENIKDWCISRQLWWGHRIPAWYC 422

Query: 537  VGKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETA 596
                 +  ++     D      + +      I+QD +VLDTWFSSALWPFST+GWP+ET 
Sbjct: 423  ----EDCGHVTVSRED----PVKCEVCGSTNIHQDENVLDTWFSSALWPFSTMGWPEET- 482

Query: 597  EDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTL 656
            ED K FYPT +L TG+DI+FFWVARM+ + +EF   +PF Y+ +HGL+RD+ GRKMSK+L
Sbjct: 483  EDLKYFYPTDVLVTGYDIIFFWVARMIFMSLEFMKEIPFKYVLIHGLVRDALGRKMSKSL 542

Query: 657  GNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKFILQNL 716
            GN IDPL+ I+++G D LRFTL +G A G D+  S E++  ++ F NKLWNA +++L NL
Sbjct: 543  GNGIDPLEVIEKYGADTLRFTLVIGNAPGNDMRFSWEKVEHSRNFANKLWNASRYVLLNL 602

Query: 717  PSQNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDV 776
              +ND+  +             L  L L + WI++  ++++  +T + +KF  G     +
Sbjct: 603  -DENDTNLY-------------LDNLALADKWILTRYNNIVKEITDNLEKFELGVAASKL 662

Query: 777  YNFFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQA 836
            Y+F W +F DWYIE SK  LY    ++  + ++VL YV +N L+LLHPFMPF+TEE+WQ 
Sbjct: 663  YDFVWSEFCDWYIELSKPVLYSDNEEAKKVTKSVLRYVLDNTLRLLHPFMPFITEEIWQN 722

Query: 837  LPHRKEALIISPWPQI--SLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIV 896
            LPH  E+++++ WP+    L  +  A KK E +    + IRN RAE +V P+K+    I 
Sbjct: 723  LPHEGESIMVAEWPKYREDLNFEEDA-KKAEIIMEAIRAIRNIRAEANVSPSKKAKVIIA 782

Query: 897  ASEEVNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVD 956
              +E ++ I E  +   ++       V    +      +++      GL A +PL +++D
Sbjct: 783  VEKEEHEKIFESGKNY-IMKLAGASEVAIERNRDNIPQKAMSAAISAGLIA-VPLEELID 842

Query: 957  ISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKR 1016
            +  E++RL +   K+  E +   + LN+ +FV+KAPE +V   REK E+    +   E+R
Sbjct: 843  LEEEIKRLEEEREKVLKEIERAQSLLNNENFVKKAPEKVVNAEREKLEKYTAMLKNIEER 879

Query: 1017 LSLLRS 1018
            LSLL S
Sbjct: 903  LSLLNS 879

BLAST of Lcy02g001710 vs. ExPASy Swiss-Prot
Match: Q3AF87 (Valine--tRNA ligase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=valS PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 1.4e-238
Identity = 422/894 (47.20%), Postives = 581/894 (64.99%), Query Frame = 0

Query: 127  EERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKG 186
            E + Y++WE  G+F  + D+   PF I MPPPNVTG LHMGHA+  T++DI+ RY RM+G
Sbjct: 15   ERKWYKYWEENGFFHTEPDERE-PFCIVMPPPNVTGQLHMGHALDNTMQDILARYKRMQG 74

Query: 187  RPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQIKR 246
              TLWLPGTDHAGIATQ  VE  L  EG+ + +LGR++F +RVW WKE YG  IT Q++ 
Sbjct: 75   FNTLWLPGTDHAGIATQAKVEEELRKEGLTKDDLGREKFLERVWAWKENYGNRITEQLRT 134

Query: 247  LGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEVEYS 306
            LGASCDW RE FTLD+  S AV E F+RL+E+GLIY+  Y+ NW P+ +T +SD+EVE+ 
Sbjct: 135  LGASCDWKRERFTLDEGCSEAVKEVFLRLYEKGLIYRDYYITNWCPHCKTTISDIEVEHL 194

Query: 307  EESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSKYVGMMAIVPMT 366
            E  G LY+I Y +  G  S YLTVATTRPET+ GD A+AVHP+DERY + +G   I+P+ 
Sbjct: 195  EREGKLYYINYPLEDG--SGYLTVATTRPETMLGDTAVAVHPEDERYRELIGKNVILPLV 254

Query: 367  YGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGLY 426
              R +P+I+D+ VDK+FGTG +KI+P HD ND+ +  +  LP + V++ D  +N+ AG Y
Sbjct: 255  -NRPIPVIADEYVDKEFGTGAVKITPAHDPNDFEVGLRHKLPQVVVLDDDAVMNENAGKY 314

Query: 427  CGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQWFVTMEPLAEK 486
             GLDR+EARKK+  DL++ GL VK+E  T  V    R   +IEP +SKQWFV M+PLAE 
Sbjct: 315  RGLDRYEARKKIVEDLKDLGLLVKEEEITHSVGHCYRCDTVIEPRLSKQWFVKMKPLAEP 374

Query: 487  ALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKNPEEDYIVA 546
            A+ A   G +  +PERF KIY +WL NI+DWCISRQLWWGHRIPVWY    +   + I +
Sbjct: 375  AIEAALTGKVKFVPERFTKIYLNWLYNIRDWCISRQLWWGHRIPVWYC---DECGEVIPS 434

Query: 547  RNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFKRFYPTTML 606
            R   ++  +      +  +++QDPDVLDTWFSSALWPFSTLGWP  T E+ K +YPT++L
Sbjct: 435  REEVKSCPKC-----QSTKVHQDPDVLDTWFSSALWPFSTLGWPQNT-EELKYYYPTSVL 494

Query: 607  ETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVIDPLDTIKE 666
             TG DI+FFWVARM+ +G+EF   VPF  + +HGL+ D+QGRKMSK+LGN +DP++ I  
Sbjct: 495  VTGRDIIFFWVARMLFMGLEFMKEVPFKEVLIHGLVLDAQGRKMSKSLGNGVDPVEVIAS 554

Query: 667  FGTDALRFTLALG-TAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPSQNDSQSWESI 726
             G D+LRF L  G T G DL    ERL   + F NKLWNA +F+L NL         E  
Sbjct: 555  HGADSLRFMLVTGNTPGNDLRFHFERLDGARNFANKLWNASRFVLMNL---------EGF 614

Query: 727  RSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYNFFWGDFADWY 786
                 ++E+    L L + WI++ L+++ID VT   D++  G+  R++Y F W +F DWY
Sbjct: 615  TPQGIKQEE----LTLADRWILARLNAVIDRVTAFLDEYELGEAARELYEFIWDEFCDWY 674

Query: 787  IEASKARLY--QSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRKEALII 846
            +E +K RLY    GGD+   A+ VL  V +  L+LLHPFMPF+TEE+WQ LPH  + +++
Sbjct: 675  VELTKPRLYGKMPGGDT---AREVLYAVLKTTLELLHPFMPFITEEIWQRLPHEGKTIML 734

Query: 847  SPWPQISLP-KQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIV-ASEEVNQYIS 906
            +PWP+     +   AVK+  +L  + + IR  RAE +V PAKR    +V A E++ + ++
Sbjct: 735  APWPKGRADYENPEAVKQMSSLMEVIREIRRLRAEVNVPPAKRGEVILVTADEQLTRLLN 794

Query: 907  EEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAELQRLSK 966
            E    +A L + +   V     P   V Q        G+  YLPL D++D+  E +RL+K
Sbjct: 795  ENAWAIAALAQSEPRVV-----PKMEVPQGALTGVAAGVTIYLPLKDLIDLEKEKERLNK 854

Query: 967  RLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1016
             L K+  E +    +LN+P F+ KAP ++V   REK      +  + E+R+ +L
Sbjct: 855  ELKKVLAEIERLNQKLNNPGFLAKAPAEVVNKEREKLTAFYREKEVLEQRIGML 874

BLAST of Lcy02g001710 vs. ExPASy Swiss-Prot
Match: Q72ZW8 (Valine--tRNA ligase OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=valS PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 3.8e-233
Identity = 416/914 (45.51%), Postives = 571/914 (62.47%), Query Frame = 0

Query: 114  TSPEIAKTFDFTS-EERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFV 173
            T   +   +D  S EE +Y+WW    YF+   D+   P+ I +PPPNVTG LH+GHA   
Sbjct: 4    TEKNLPTKYDHMSVEEGLYQWWLEGKYFEATGDEKKQPYTIVIPPPNVTGKLHLGHAWDT 63

Query: 174  TLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEW 233
            TL+DI+ R  RM+G   LWLPG DHAGIATQ  VE  L  EGI R +LGR++F ++ WEW
Sbjct: 64   TLQDILTRTKRMQGYDVLWLPGMDHAGIATQAKVEGKLREEGISRYDLGREKFLEKAWEW 123

Query: 234  KEKYGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP 293
            KE+Y   I  Q  ++G   D++RE FTLD+ LS AV + FV+L+E+GLIY+G Y++NW P
Sbjct: 124  KEEYASHIRQQWGKVGLGLDYSRERFTLDEGLSDAVNKVFVQLYEKGLIYRGEYIINWDP 183

Query: 294  NLQTAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDER 353
              +TA+SD+EV + E  G  YH+ Y +  G  S ++ +ATTRPET+ GD A+AVHP+D+R
Sbjct: 184  ATRTALSDIEVIHKEVQGAFYHMNYPLTDG--SGHIRLATTRPETMLGDTAVAVHPEDDR 243

Query: 354  YSKYVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 413
            Y   +G    +P+  GR +PII+D+ V+KDFGTGV+KI+P HD ND+ +  +  LP + V
Sbjct: 244  YKHLIGKTVTLPIV-GREIPIIADEYVEKDFGTGVVKITPAHDPNDFEVGNRHDLPRILV 303

Query: 414  MNKDGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLV 473
            MN+DGT+N+ AG Y G+DRFE RK L  DL+E G+ V+ E H   V  S+R G ++EP +
Sbjct: 304  MNEDGTMNEKAGKYNGMDRFECRKALVKDLQEAGVLVEIEPHMHSVGHSERSGAVVEPYL 363

Query: 474  SKQWFVTMEPLAEK--ALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIP 533
            S QWFV M PLAEK  AL+  E   +T +PERFE  Y  W+ NI DWCISRQLWWGHRIP
Sbjct: 364  STQWFVKMAPLAEKAVALQQKEEEKVTFVPERFENTYLRWMENIHDWCISRQLWWGHRIP 423

Query: 534  VWYIVGKNPEEDYIVARNADEALEQARKKYGKDVEIY-QDPDVLDTWFSSALWPFSTLGW 593
             WY   K   E Y+      EA          D+E + QD DVLDTWFSSALWPFSTLGW
Sbjct: 424  AWY--HKETGEVYV----GTEA--------PADIENWNQDNDVLDTWFSSALWPFSTLGW 483

Query: 594  PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 653
            P+E A DFKR+Y T  L TG+DI+FFWV+RM+  G+EFTG  PF  + +HGL+RD QGRK
Sbjct: 484  PNEDAADFKRYYSTDALVTGYDIIFFWVSRMIFQGLEFTGERPFKDVLIHGLVRDEQGRK 543

Query: 654  MSKTLGNVIDPLDTIKEFGTDALRFTLALGTA-GQDLNLSTERLTSNKAFTNKLWNAGKF 713
            MSK+LGN IDP++ I+++G DA+RF L+ G+A GQDL  S E++ S   F NK+WNA +F
Sbjct: 544  MSKSLGNGIDPMEVIEKYGADAMRFFLSTGSAPGQDLRFSMEKVESTWNFINKIWNASRF 603

Query: 714  ILQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGD 773
            +L N+               ++E+ D   +  + + WI++ L+  I+ VT + DK+ FG+
Sbjct: 604  VLMNMD------------DMKYEEIDLTGEKSVADKWILTRLNETIESVTRNMDKYEFGE 663

Query: 774  VGRDVYNFFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTE 833
             GR +YNF W DF DWYIE +K  LY     +    +++L YV +  ++LLHPFMPFVTE
Sbjct: 664  AGRSLYNFIWDDFCDWYIEMAKLPLYGEDEAAKKTTRSILAYVLDQTMRLLHPFMPFVTE 723

Query: 834  ELWQALPHRKEALIISPWPQISLPKQ-ASAVKKFENLKLLTKVIRNARAEYSVEPAKRIS 893
            ++WQ LPH  E++ ++ WP +    Q A A  +   L  + + +RN RAE +   +K++ 
Sbjct: 724  KIWQHLPHEGESITVAAWPTVREDLQDAEAAAEMHLLVDIIRSVRNIRAEVNTPMSKKVQ 783

Query: 894  ASIVASEEVNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGE--------G 953
              I A +E          VLA LT+   +   F N  P  +     L A E        G
Sbjct: 784  MQIKAKDEA---------VLAQLTKNSSYIERFCN--PSELTIQTDLQAPEKAMTAIVTG 843

Query: 954  LEAYLPLADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAE 1013
             E +LPLAD++++  E  RL K L K   E +    +L++  FV KAP  ++ G R K +
Sbjct: 844  AELFLPLADLINLDEERARLEKELEKFDKEVERVQKKLSNQGFVAKAPAAVIEGERAKEQ 877

BLAST of Lcy02g001710 vs. ExPASy Swiss-Prot
Match: Q9X2D7 (Valine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=valS PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 5.0e-233
Identity = 416/889 (46.79%), Postives = 573/889 (64.45%), Query Frame = 0

Query: 127  EERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKG 186
            E + YR+WE +GYF P+   +   F I +PPPN+TG +HMGHA+ +TL+DI+VRY RMKG
Sbjct: 14   ETKWYRYWEEKGYFTPKGVGEK--FSIVIPPPNITGRIHMGHALNITLQDIVVRYKRMKG 73

Query: 187  RPTLWLPGTDHAGIATQLVVER-MLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQIK 246
               LW+PG DHAGIATQ  VE+ +L ++G  R E+GR++F +  WEW  KY   I  QIK
Sbjct: 74   YDVLWVPGEDHAGIATQNAVEKFLLQTQGKTREEIGREKFLEITWEWANKYRREIREQIK 133

Query: 247  RLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEVEY 306
             LGAS DWTRE FTLD+ LSRAV + FV L+ +GLIY+G Y+VNW P  +T +SD EVE+
Sbjct: 134  ALGASVDWTRERFTLDEGLSRAVRKVFVELYRKGLIYRGKYIVNWCPRCKTVLSDEEVEH 193

Query: 307  SEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSKYVGMMAIVPM 366
             E    LY++KY V      +Y+ VATTRPET+ GD A+AVHP+DERY  +VG   I+P+
Sbjct: 194  KEHKSKLYYVKYPVK--DSDEYIVVATTRPETMLGDTAVAVHPEDERYKNFVGKTLILPL 253

Query: 367  TYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNKVAGL 426
              GR +P+++DK VD  FGTG +K++P HD NDYL+A++  LP++ + + +  +N+  G 
Sbjct: 254  V-GREIPVVADKYVDPKFGTGAVKVTPAHDPNDYLIAQRHNLPMIEIFDDNARINENGGK 313

Query: 427  YCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQWFVTMEPLAE 486
            Y GLDR+EAR+K+  DLEE G  VK E +T  V    R   +IEP +S QWFV+ +PLA+
Sbjct: 314  YKGLDRYEAREKIVKDLEEQGFLVKIEDYTHSVGHCYRCDTVIEPKLSDQWFVSTKPLAK 373

Query: 487  KALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKNPEEDYIV 546
            +A+ AVE G++   PER+ K+Y +W+  I+DWCISRQLWWGHRIPVWY       +D   
Sbjct: 374  RAIEAVENGEIRFFPERWTKVYLNWMYEIRDWCISRQLWWGHRIPVWYC------QDCGH 433

Query: 547  ARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFKRFYPTTM 606
               ++E +E+  K    +++  QD DVLDTWFSSALWPFSTLGWP+ET ED KR+YPT +
Sbjct: 434  LNVSEEDVEKCEKCGSTNLK--QDEDVLDTWFSSALWPFSTLGWPEET-EDLKRYYPTDL 493

Query: 607  LETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVIDPLDTIK 666
            L TG DI+FFWVARM+M+G EF    PFS++Y+H L+RD  GRKMSK+LGN IDPL+ I 
Sbjct: 494  LVTGFDIIFFWVARMIMMGYEFMNDKPFSHVYIHQLVRDKYGRKMSKSLGNGIDPLEVID 553

Query: 667  EFGTDALRFTLA-LGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPSQNDSQSWES 726
            E+G D +RFTLA L   G+D+ L      + K F NK+WNA +F+L NL           
Sbjct: 554  EYGADPMRFTLAILAAQGRDIKLDPRYFDAYKKFANKIWNATRFVLMNL----------- 613

Query: 727  IRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYNFFWGDFADW 786
                E  KE  L  L   + WI++ L+  ++ VT + + + F    R +YNFFW DF DW
Sbjct: 614  ----EDYKEVPLENLKTVDKWILTRLNKTVEEVTNALENYDFNIAARTIYNFFWDDFCDW 673

Query: 787  YIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALPHRKEALIIS 846
            YIEASK RL     +   L Q VL+ V +  L+LLHPFMPF+TEELWQ LP   E++ I+
Sbjct: 674  YIEASKPRLKT---EERNLVQTVLVKVLDASLRLLHPFMPFLTEELWQKLPVAGESITIA 733

Query: 847  PWPQISLPK-QASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEEVNQYISEE 906
             WP+I       +A K+F  L  + + +RN RAE ++  ++R+   I   E     ++EE
Sbjct: 734  KWPEIERELIDETAEKEFTRLMNMVRGVRNVRAEMNLPQSQRVKVYIKGYE-----VTEE 793

Query: 907  KEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAELQRLSKRL 966
            +E+L L T  ++  V F N  P    ++      E +EAY+ L  ++D   E +RL + +
Sbjct: 794  EELL-LKTLGNIEEVSFVNEKP---PKTATAYVEEEIEAYVDLGGLIDFEKEKERLKQIM 853

Query: 967  TKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRL 1013
             K++ E D    +L +  FVEKAPE++V   +EK    +E++A  E  L
Sbjct: 854  EKIQKEIDRLEKKLANKDFVEKAPEEVVEETKEKLNTNRERLARLESIL 861

BLAST of Lcy02g001710 vs. ExPASy TrEMBL
Match: A0A6J1IZ88 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1)

HSP 1 Score: 1901.7 bits (4925), Expect = 0.0e+00
Identity = 933/968 (96.38%), Postives = 950/968 (98.14%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK RR+FGLSRFHFRR Q R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKCRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARK GLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKFGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGG+SVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGNSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQI LP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKA+EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. ExPASy TrEMBL
Match: A0A6J1F580 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1)

HSP 1 Score: 1899.8 bits (4920), Expect = 0.0e+00
Identity = 932/968 (96.28%), Postives = 949/968 (98.04%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK+RR+FGLSRFHFRR   R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLLHRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLW DLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWVDLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHS ID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSQIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQI LP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKA+EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. ExPASy TrEMBL
Match: A0A1S3C4H7 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 1862.0 bits (4822), Expect = 0.0e+00
Identity = 914/968 (94.42%), Postives = 939/968 (97.00%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLL MAFSGPS L P SA RLNPL+ TK RR+F LSRF FRR   R LAVAASANGVFTS
Sbjct: 1    MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIY+WWESQGYF+P +DQD+VPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAV+EAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGS SDYLTVATTRPETLFGDVAIAV+PQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSSSDYLTVATTRPETLFGDVAIAVNPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGM AIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMTAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLW DLEE GLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWTDLEEMGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WFVTMEPLAEKALRAVE+G+LTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFVTMEPLAEKALRAVEKGELTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEALEQA+KKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEALEQAQKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVM+GIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMMGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI SFEFEK+DCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRDVYN
Sbjct: 661  QNDSQSWDSILSFEFEKDDCLLKLPLPECWIVSELHSLIDVVTISYDKFFFGDVGRDVYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVF+NILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFKNILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            +RKEALIIS WPQISLP+QASAVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  NRKEALIISHWPQISLPRQASAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYIS EKEVLALLTRLDL NV FANSPPGNVDQSVHLVAGEGLEAYLPLADM+DISAE
Sbjct: 841  VNQYISGEKEVLALLTRLDLRNVRFANSPPGNVDQSVHLVAGEGLEAYLPLADMIDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            +QRLSKRLTKMK EYDGFIARL+SPSFVEKAP+DIVRGVREKA EAKEKIALTEKRLSLL
Sbjct: 901  VQRLSKRLTKMKMEYDGFIARLSSPSFVEKAPKDIVRGVREKAVEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RST PVPD
Sbjct: 961  RSTAPVPD 968

BLAST of Lcy02g001710 vs. ExPASy TrEMBL
Match: A0A6J1CQF3 (Valyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111013225 PE=3 SV=1)

HSP 1 Score: 1847.8 bits (4785), Expect = 0.0e+00
Identity = 909/968 (93.90%), Postives = 934/968 (96.49%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQM FSGPSLLPP   YRLNPL+ T+ R AF LSR HF R +     VAASANGVFTS
Sbjct: 1    MLLQMPFSGPSLLPP---YRLNPLLFTRHRPAFRLSRSHFCRLRPTQFTVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PE+AKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEVAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPSLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLY+IKYRVAGGS+SDYLT+ATTRPETLFGDVAIAV+PQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYYIKYRVAGGSKSDYLTIATTRPETLFGDVAIAVNPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDK+VDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKHVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLY GLDRFEARKKLWADLEETGLAV+KEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYRGLDRFEARKKLWADLEETGLAVRKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWY+V
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYVV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEE+YIVARNA EALEQA KKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEEYIVARNAGEALEQAHKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDS+SWESI SFEFEKEDCLLKL LPECWIVSELHSLIDMVTTSYDKFFFGD+GRDVYN
Sbjct: 661  QNDSRSWESILSFEFEKEDCLLKLSLPECWIVSELHSLIDMVTTSYDKFFFGDIGRDVYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGG SV LAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGHSVTLAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQ SLP+QASAVKKFENLKLLT+ IRNARAEYSVE AKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQSSLPRQASAVKKFENLKLLTRAIRNARAEYSVESAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLAL TRLDL+NV+FANSPPGNVDQSVHLVAGEGLEAYLPLADMVDIS E
Sbjct: 841  VNQYISEEKEVLALFTRLDLNNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISVE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRL+KMK EYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIAL EKR  LL
Sbjct: 901  LQRLSKRLSKMKMEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALIEKRFFLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 965

BLAST of Lcy02g001710 vs. ExPASy TrEMBL
Match: A0A1S3C571 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 1787.3 bits (4628), Expect = 0.0e+00
Identity = 881/968 (91.01%), Postives = 906/968 (93.60%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLL MAFSGPS L P SA RLNPL+ TK RR+F LSRF FRR   R LAVAASANGVFTS
Sbjct: 1    MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIY+WWESQGYF+P +DQD+VPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAV+EAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGS SDYLTVATTRPETLFGDVAIAV+PQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSSSDYLTVATTRPETLFGDVAIAVNPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGM AIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMTAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLW DLEE GLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWTDLEEMGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WFVTMEPLAEKALRAVE+G+LTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFVTMEPLAEKALRAVEKGELTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEALEQA+KKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEALEQAQKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVM+GIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMMGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI SFEFEK+DCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRDVYN
Sbjct: 661  QNDSQSWDSILSFEFEKDDCLLKLPLPECWIVSELHSLIDVVTISYDKFFFGDVGRDVYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVF+NILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFKNILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            +RKEALIIS WPQISLP+QASAVKKFENLKLL                            
Sbjct: 781  NRKEALIISHWPQISLPRQASAVKKFENLKLL---------------------------- 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
                   EKEVLALLTRLDL NV FANSPPGNVDQSVHLVAGEGLEAYLPLADM+DISAE
Sbjct: 841  ------GEKEVLALLTRLDLRNVRFANSPPGNVDQSVHLVAGEGLEAYLPLADMIDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            +QRLSKRLTKMK EYDGFIARL+SPSFVEKAP+DIVRGVREKA EAKEKIALTEKRLSLL
Sbjct: 901  VQRLSKRLTKMKMEYDGFIARLSSPSFVEKAPKDIVRGVREKAVEAKEKIALTEKRLSLL 934

Query: 1016 RSTVPVPD 1024
            RST PVPD
Sbjct: 961  RSTAPVPD 934

BLAST of Lcy02g001710 vs. NCBI nr
Match: KAG6581186.1 (Valine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1907.9 bits (4941), Expect = 0.0e+00
Identity = 936/968 (96.69%), Postives = 952/968 (98.35%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK+RR+FGLSRFHFRR Q R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLQHRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQISLP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQISLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKA EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKANEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. NCBI nr
Match: XP_023528105.1 (LOW QUALITY PROTEIN: valine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1905.2 bits (4934), Expect = 0.0e+00
Identity = 934/968 (96.49%), Postives = 951/968 (98.24%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK+RR+FGLSRFHFRR Q R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQI LP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRG REKA+EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGAREKADEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. NCBI nr
Match: XP_022983417.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1901.7 bits (4925), Expect = 0.0e+00
Identity = 933/968 (96.38%), Postives = 950/968 (98.14%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK RR+FGLSRFHFRR Q R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKCRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARK GLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKFGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGG+SVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGNSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQI LP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKA+EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. NCBI nr
Match: XP_022935322.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1899.8 bits (4920), Expect = 0.0e+00
Identity = 932/968 (96.28%), Postives = 949/968 (98.04%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK+RR+FGLSRFHFRR   R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLLHRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLW DLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWVDLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHS ID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSQIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQI LP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQILLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PPGNVDQSVHLVAGEGLEAYLPLADMVDISAE
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 900

Query: 956  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 1015
            LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKA+EAKEKIALTEKRLSLL
Sbjct: 901  LQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIALTEKRLSLL 960

Query: 1016 RSTVPVPD 1024
            RSTVPVPD
Sbjct: 961  RSTVPVPD 968

BLAST of Lcy02g001710 vs. NCBI nr
Match: KAG7017926.1 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1897.1 bits (4913), Expect = 0.0e+00
Identity = 936/987 (94.83%), Postives = 953/987 (96.56%), Query Frame = 0

Query: 56   MLLQMAFSGPSLLPPCSAYRLNPLILTKQRRAFGLSRFHFRRPQLRLLAVAASANGVFTS 115
            MLLQMAFSGPSLL P SAYRLNPLI TK+RR+FGLSRFHFRR Q R LAVAASANGVFTS
Sbjct: 1    MLLQMAFSGPSLLHPSSAYRLNPLIFTKRRRSFGLSRFHFRRLQHRQLAVAASANGVFTS 60

Query: 116  PEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 175
            PEIAKTFDF SEERIYRWWESQGYF+P FDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61   PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 176  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 235
            DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121  DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 236  YGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQ 295
            YGGTITNQIKRLGASCDWT+EHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181  YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 296  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDERYSK 355
            TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQD+RYSK
Sbjct: 241  TAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQDDRYSK 300

Query: 356  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 415
            YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK
Sbjct: 301  YVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNVMNK 360

Query: 416  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 475
            DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ
Sbjct: 361  DGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPLVSKQ 420

Query: 476  WFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 535
            WF+TMEPLAEKALRAVE+GDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV
Sbjct: 421  WFMTMEPLAEKALRAVEKGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIV 480

Query: 536  GKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 595
            GKNPEEDYIVARNADEA EQA KKYGK VEIYQDPDVLDTWFSSALWPFSTLGWPDETAE
Sbjct: 481  GKNPEEDYIVARNADEAHEQAHKKYGKGVEIYQDPDVLDTWFSSALWPFSTLGWPDETAE 540

Query: 596  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 655
            DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG
Sbjct: 541  DFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLG 600

Query: 656  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPS 715
            NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLP+
Sbjct: 601  NVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFILQNLPT 660

Query: 716  QNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDVGRDVYN 775
            QNDSQSW+SI S+EFEKEDCLLKLPLPECWIVSELHSLID+VT SYDKFFFGDVGRD+YN
Sbjct: 661  QNDSQSWKSILSYEFEKEDCLLKLPLPECWIVSELHSLIDVVTASYDKFFFGDVGRDIYN 720

Query: 776  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 835
            FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP
Sbjct: 721  FFWGDFADWYIEASKARLYQSGGDSVALAQAVLLYVFENILKLLHPFMPFVTEELWQALP 780

Query: 836  HRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISASIVASEE 895
            HRKEALIISPWPQISLP+QA AVKKFENLKLLTK IRNARAEYSVEPAKRISASIVASEE
Sbjct: 781  HRKEALIISPWPQISLPRQAGAVKKFENLKLLTKAIRNARAEYSVEPAKRISASIVASEE 840

Query: 896  VNQYISEEKEVLALLTRLDLHNVYFANSPP-------------------GNVDQSVHLVA 955
            VNQYISEEKEVLALLTRLDLHNV+FAN+PP                   GNVDQSVHLVA
Sbjct: 841  VNQYISEEKEVLALLTRLDLHNVHFANAPPGMSITRSVEALFCLMICFTGNVDQSVHLVA 900

Query: 956  GEGLEAYLPLADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVRE 1015
            GEGLEAYLPLADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVRE
Sbjct: 901  GEGLEAYLPLADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVRE 960

Query: 1016 KAEEAKEKIALTEKRLSLLRSTVPVPD 1024
            KA+EAKEKIALTEKRLSLLRSTVPVPD
Sbjct: 961  KADEAKEKIALTEKRLSLLRSTVPVPD 987

BLAST of Lcy02g001710 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 1586.2 bits (4106), Expect = 0.0e+00
Identity = 769/970 (79.28%), Postives = 866/970 (89.28%), Query Frame = 0

Query: 56   MLLQMAFSGP----SLLPPCSAYRLNPLILT-KQRRAFGLSRFHFRRPQLRL-LAVAASA 115
            M+L+ AFS P    +LL P S ++LN L  T ++RR    SR +    Q R   + AAS 
Sbjct: 1    MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRLISPSRLNSIFSQRRFSFSAAASG 60

Query: 116  NGVFTSPEIAKTFDFTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHA 175
            N VFTSPE +KTFDF+SEE+IY+WWESQGYFKP FDQ   PFVI MPPPNVTGSLHMGHA
Sbjct: 61   NNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGHA 120

Query: 176  MFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRV 235
            MFVTLEDIMVRYNRM GRPTLWLPGTDHAGIATQLVVE+MLASEGIKRV+LGRDEFTKRV
Sbjct: 121  MFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKRV 180

Query: 236  WEWKEKYGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVN 295
            WEWKEKYGGTITNQIKRLGASCDW+RE FTLD+QLSRAVVEAFV+LH++GLIYQGSYMVN
Sbjct: 181  WEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVN 240

Query: 296  WSPNLQTAVSDLEVEYSEESGTLYHIKYRVAGGSRSDYLTVATTRPETLFGDVAIAVHPQ 355
            WSPNLQTAVSDLEVEYSEE G LYHIKYRVAG    D+LT+ATTRPETLFGDVA+AVHP+
Sbjct: 241  WSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAGS--PDFLTIATTRPETLFGDVALAVHPE 300

Query: 356  DERYSKYVGMMAIVPMTYGRHVPIISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPI 415
            D+RYSKYVG  AIVPMTYGRHVPII+DK VDKDFGTGVLKISPGHDHNDYLLARKLGLPI
Sbjct: 301  DDRYSKYVGQTAIVPMTYGRHVPIIADKYVDKDFGTGVLKISPGHDHNDYLLARKLGLPI 360

Query: 416  LNVMNKDGTLNKVAGLYCGLDRFEARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIE 475
            LNVMNKD TLN VAGL+CGLDRFE R+KLWADLEE GLAVKKE HTLRVPRSQRGGE+IE
Sbjct: 361  LNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVIE 420

Query: 476  PLVSKQWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRI 535
            PLVSKQWFV M+PLAEKAL AVE  +LTIIPERFEKIYNHWL+NIKDWCISRQLWWGHRI
Sbjct: 421  PLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHRI 480

Query: 536  PVWYIVGKNPEEDYIVARNADEALEQARKKYGKDVEIYQDPDVLDTWFSSALWPFSTLGW 595
            PVWY+VGK+ EEDYIVA++A+EALE+A +KYGKDVEIYQDPDVLDTWFSS+LWPFSTLGW
Sbjct: 481  PVWYVVGKDCEEDYIVAKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLGW 540

Query: 596  PDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSYIYLHGLIRDSQGRK 655
            PD  A+DF  FYPT MLETGHDILFFWVARMVM+GIEFTGTVPFS++YLHGLIRDSQGRK
Sbjct: 541  PDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRK 600

Query: 656  MSKTLGNVIDPLDTIKEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFI 715
            MSK+LGNVIDPLDTIK+FGTDALRFT+ALGTAGQDLNLSTERLT+NKAFTNKLWNAGKF+
Sbjct: 601  MSKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLSTERLTANKAFTNKLWNAGKFV 660

Query: 716  LQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKFFFGDV 775
            L +LPS +D+ +WE++   + +KE+ LL LPLPECW VS+LH LID VT SY+K FFGDV
Sbjct: 661  LHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGDV 720

Query: 776  GRDVYNFFWGDFADWYIEASKARLYQSGGDSVALA-QAVLLYVFENILKLLHPFMPFVTE 835
            GR+ Y+FFW DFADWYIEASK+RLY SGG+SV+LA QAVLLYVFENILKLLHPFMPFVTE
Sbjct: 721  GRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTE 780

Query: 836  ELWQALPHRKEALIISPWPQISLPKQASAVKKFENLKLLTKVIRNARAEYSVEPAKRISA 895
            +LWQALP+RKEALI+SPWPQ SLP+   ++K+FENL+ LT+ IRNARAEYSVEP KRISA
Sbjct: 781  DLWQALPYRKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRISA 840

Query: 896  SIVASEEVNQYISEEKEVLALLTRLDLHNVYFANSPPGNVDQSVHLVAGEGLEAYLPLAD 955
            S+V S EV +YIS+EKEVLALL+RLDL+NV+F+N+PPG+ + SVHLVA EGLEAYLPLA 
Sbjct: 841  SVVGSAEVIEYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEAYLPLAA 900

Query: 956  MVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKAEEAKEKIALT 1015
            MVDIS+E+QR+SKRL+KM+TEYD  I RL+SP FVEKAPE++VRGV+E+ EE +EKI LT
Sbjct: 901  MVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLT 960

Query: 1016 EKRLSLLRST 1019
            + RL  L+ST
Sbjct: 961  KARLDFLKST 968

BLAST of Lcy02g001710 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 642.9 bits (1657), Expect = 4.4e-184
Identity = 385/987 (39.01%), Postives = 545/987 (55.22%), Query Frame = 0

Query: 115  SPEIAKTFD-FTSEERIYRWWESQGYFKPQFDQDSVPFVISMPPPNVTGSLHMGHAMFVT 174
            S ++AK +   T E+  Y WWE    FK        PFVI +PPPNVTG+LH+GHA+   
Sbjct: 133  SSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSA 192

Query: 175  LEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVE-RMLASEGIKRVELGRDEFTKRVWEW 234
            +ED ++R+ RM G   LW+PG DHAGIATQ+VVE +++   G+ R ++GR+EF K VW+W
Sbjct: 193  IEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFVKEVWKW 252

Query: 235  KEKYGGTITNQIKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSP 294
            K +YGGTI  Q++RLGAS DW+RE FT+D+Q S+AV EAFVRL++ GLIY+   +VNW  
Sbjct: 253  KNQYGGTILTQLRRLGASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIRLVNWDC 312

Query: 295  NLQTAVSDLEVEYSE----------------ESGTLYHIKYRVAGGSRSDYLTVATTRPE 354
             L+TA+SD+EVEY +                E G L    Y + GG     + VATTR E
Sbjct: 313  ILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFGLLTSFAYPLEGGLGE--VIVATTRVE 372

Query: 355  TLFGDVAIAVHPQDERYSKYVGMMAIVPMTYGRHVPIISDK-NVDKDFGTGVLKISPGHD 414
            T+ GD AIA+HP D RY    G  A+ P   GR +PII D   VD +FGTG +KI+P HD
Sbjct: 373  TMLGDTAIAIHPDDARYKHLHGKFAVHPFN-GRKLPIICDGILVDPNFGTGCVKITPAHD 432

Query: 415  HNDYLLARKLGLPILNVMNKDGTLNKVAGL-YCGLDRFEARKKLWADLEETGLAVKKEAH 474
             ND  + ++  L  +N+   DG +N   G  + G+ RF AR+ +   L++ GL    + +
Sbjct: 433  PNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPRFAAREAVVEALQKQGLYRGAKNN 492

Query: 475  TLRVPRSQRGGEIIEPLVSKQWFVTMEPLAEKALRAV---ERGDLTIIPERFEKIYNHWL 534
             +R+    R  ++IEP++  QW+V    + ++AL      E   L  +P+++   +  WL
Sbjct: 493  EMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVAITDENKKLEFVPKQYTAEWRRWL 552

Query: 535  SNIKDWCISRQLWWGHRIPVWYIVGKNPE--------EDYIVARNADEALEQARKKY-GK 594
             NI+DWCISRQLWWGHRIP WY   +  +        + ++VAR  D+A E+A +K+ GK
Sbjct: 553  ENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYSDHWVVARTEDDAREEAAQKFLGK 612

Query: 595  DVEIYQDPDVLDTWFSSALWPFSTLGWPDETAEDFKRFYPTTMLETGHDILFFWVARMVM 654
              E+ +DPDVLDTWFSS L+P S LGWPD T +DFK FYPT++LETGHDILFFWVARMVM
Sbjct: 613  KFELTRDPDVLDTWFSSGLFPLSVLGWPDVT-DDFKAFYPTSVLETGHDILFFWVARMVM 672

Query: 655  LGIEFTGTVPFSYIYLHGLIRDSQGRKMSKTLGNVIDPLDTIK----------------- 714
            +G++  G VPFS +Y H +IRD+ GRKMSK+LGNVIDPL+ I                  
Sbjct: 673  MGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDPLEVINGVTLEGLHKRLEEGNLD 732

Query: 715  --------------------EFGTDALRFTLALGTAGQD-LNLSTERLTSNKAFTNKLWN 774
                                E GTDALRF L   TA  D +NL   R+   + + NKLWN
Sbjct: 733  PKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQSDKINLDILRVVGYRQWCNKLWN 792

Query: 775  AGKFILQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWIVSELHSLIDMVTTSYDKF 834
            A +F +  L    D  +     S E         +P    WI+S L+  I     S D F
Sbjct: 793  AVRFAMMKL---GDGYTPPQTLSPE--------TMPFSCQWILSVLNKAISKTVVSLDAF 852

Query: 835  FFGDVGRDVYNFFWGDFADWYIEASKARLYQSGGDSV-----ALAQAVLLYVFENILKLL 894
             F D    +Y ++   F D YIEA K   Y +G +       A AQ  L    E  L+LL
Sbjct: 853  EFSDAANTIYAWWQYQFCDVYIEAIKP--YFAGDNPTFASERAHAQHALWISLETGLRLL 912

Query: 895  HPFMPFVTEELWQALP-----HRKEALIISPWPQ-ISLPKQASAVKKFENLKLLTKVIRN 954
            HPFMPFVTEELWQ LP      RK +++I  +P  I          + + +    K +R 
Sbjct: 913  HPFMPFVTEELWQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTVLATVKCMRA 972

Query: 955  ARAE-YSVEPAKRISASIVASEEVNQYI--SEEKEV--LALLTRLDLHNVYFANSPPGNV 1014
             RA     +  +R+ A  +    V   I  S E E+  LA L+ L++ +     +PPG+ 
Sbjct: 973  LRAGLLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKGQHAAPPGSS 1032

Query: 1015 DQSVHLVAGEGLEAYLPLADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPE 1016
             ++V+    E L+ YL +   ++  AE +++  ++ +++ + +     ++  ++ EK P 
Sbjct: 1033 VETVN----ENLKVYLEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYEEKVPA 1092

BLAST of Lcy02g001710 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 238.0 bits (606), Expect = 3.3e-62
Identity = 200/811 (24.66%), Postives = 349/811 (43.03%), Query Frame = 0

Query: 125 TSEERIYRWWESQGYFKPQFDQDS-VPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNR 184
           T E  + + WE    FK   D ++   F++   PP   G LHMGHA+   L+DI+ RY  
Sbjct: 121 TREPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKL 180

Query: 185 MKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQ 244
           ++     ++PG D  G+  +L V + L  E   R EL   +   +  ++ +    T    
Sbjct: 181 LQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMES 240

Query: 245 IKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEV 304
            KR G   DW   + TLD +   A +E F ++  +G IY+G   V+WSP+ +TA+++ E+
Sbjct: 241 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 300

Query: 305 EYSEE--SGTLYHIKYRVAGGSRSD---------YLTVATTRPETLFGDVAIAVH----- 364
           EY E   S ++Y I +++ GG+++          YL V TT P T+  + A+AV+     
Sbjct: 301 EYPEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 360

Query: 365 --------PQDE-----------------RYSKYV--------------GMMAIVPMTY- 424
                    +DE                 +   +V              G+   +  T+ 
Sbjct: 361 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFL 420

Query: 425 --------------GRHVP-IISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 484
                          R  P +I    +  + GTG++  +PGH   DY    K GLP+++ 
Sbjct: 421 GSDLENCRYTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSP 480

Query: 485 MNKDGTLNKVAGLYCGLDRF-EARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPL 544
           ++ +G   + AG + GL    E    + + L+E    V +E++  + P   R  +     
Sbjct: 481 VDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFR 540

Query: 545 VSKQWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPV 604
            ++QWF ++E      + A+   ++  +P +     +   S+  DWCISRQ  WG  IP 
Sbjct: 541 ATEQWFASVEGFRTATMDAI--NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPA 600

Query: 605 WY-IVGKNP---EEDY-----IVARNADEAL------EQARKKY-GKDVEIYQDPDVLDT 664
           +Y +  K P   EE       I+++   +A       +   +KY  K  +  +  D +D 
Sbjct: 601 FYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDV 660

Query: 665 WFSSALWPFSTLGWPDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSY 724
           WF S         W     +     +P  +   G D    W    ++  I   G  P+S 
Sbjct: 661 WFDSG------SSWAGVLGKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSA 720

Query: 725 IYLHGLIRDSQGRKMSKTLGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLN 784
           +  HG + D +G KMSK+LGNV+DP   I+          +G D +R  ++      D+ 
Sbjct: 721 VITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVL 780

Query: 785 LSTERLTSNKAFTNKLWNAGKFILQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWI 836
           +  + L        KL    +++L NL        W    +  ++       LP+ +   
Sbjct: 781 IGPQILRQMSDIYRKLRGTLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHA 840

BLAST of Lcy02g001710 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 238.0 bits (606), Expect = 3.3e-62
Identity = 200/811 (24.66%), Postives = 349/811 (43.03%), Query Frame = 0

Query: 125 TSEERIYRWWESQGYFKPQFDQDS-VPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNR 184
           T E  + + WE    FK   D ++   F++   PP   G LHMGHA+   L+DI+ RY  
Sbjct: 121 TREPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKL 180

Query: 185 MKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQ 244
           ++     ++PG D  G+  +L V + L  E   R EL   +   +  ++ +    T    
Sbjct: 181 LQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMES 240

Query: 245 IKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEV 304
            KR G   DW   + TLD +   A +E F ++  +G IY+G   V+WSP+ +TA+++ E+
Sbjct: 241 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 300

Query: 305 EYSEE--SGTLYHIKYRVAGGSRSD---------YLTVATTRPETLFGDVAIAVH----- 364
           EY E   S ++Y I +++ GG+++          YL V TT P T+  + A+AV+     
Sbjct: 301 EYPEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 360

Query: 365 --------PQDE-----------------RYSKYV--------------GMMAIVPMTY- 424
                    +DE                 +   +V              G+   +  T+ 
Sbjct: 361 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFL 420

Query: 425 --------------GRHVP-IISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 484
                          R  P +I    +  + GTG++  +PGH   DY    K GLP+++ 
Sbjct: 421 GSDLENCRYTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSP 480

Query: 485 MNKDGTLNKVAGLYCGLDRF-EARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPL 544
           ++ +G   + AG + GL    E    + + L+E    V +E++  + P   R  +     
Sbjct: 481 VDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFR 540

Query: 545 VSKQWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPV 604
            ++QWF ++E      + A+   ++  +P +     +   S+  DWCISRQ  WG  IP 
Sbjct: 541 ATEQWFASVEGFRTATMDAI--NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPA 600

Query: 605 WY-IVGKNP---EEDY-----IVARNADEAL------EQARKKY-GKDVEIYQDPDVLDT 664
           +Y +  K P   EE       I+++   +A       +   +KY  K  +  +  D +D 
Sbjct: 601 FYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDV 660

Query: 665 WFSSALWPFSTLGWPDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSY 724
           WF S         W     +     +P  +   G D    W    ++  I   G  P+S 
Sbjct: 661 WFDSG------SSWAGVLGKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSA 720

Query: 725 IYLHGLIRDSQGRKMSKTLGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLN 784
           +  HG + D +G KMSK+LGNV+DP   I+          +G D +R  ++      D+ 
Sbjct: 721 VITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVL 780

Query: 785 LSTERLTSNKAFTNKLWNAGKFILQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWI 836
           +  + L        KL    +++L NL        W    +  ++       LP+ +   
Sbjct: 781 IGPQILRQMSDIYRKLRGTLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHA 840

BLAST of Lcy02g001710 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 238.0 bits (606), Expect = 3.3e-62
Identity = 200/811 (24.66%), Postives = 349/811 (43.03%), Query Frame = 0

Query: 125  TSEERIYRWWESQGYFKPQFDQDS-VPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNR 184
            T E  + + WE    FK   D ++   F++   PP   G LHMGHA+   L+DI+ RY  
Sbjct: 307  TREPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKL 366

Query: 185  MKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQ 244
            ++     ++PG D  G+  +L V + L  E   R EL   +   +  ++ +    T    
Sbjct: 367  LQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMES 426

Query: 245  IKRLGASCDWTREHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEV 304
             KR G   DW   + TLD +   A +E F ++  +G IY+G   V+WSP+ +TA+++ E+
Sbjct: 427  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 486

Query: 305  EYSEE--SGTLYHIKYRVAGGSRSD---------YLTVATTRPETLFGDVAIAVH----- 364
            EY E   S ++Y I +++ GG+++          YL V TT P T+  + A+AV+     
Sbjct: 487  EYPEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 546

Query: 365  --------PQDE-----------------RYSKYV--------------GMMAIVPMTY- 424
                     +DE                 +   +V              G+   +  T+ 
Sbjct: 547  SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFL 606

Query: 425  --------------GRHVP-IISDKNVDKDFGTGVLKISPGHDHNDYLLARKLGLPILNV 484
                           R  P +I    +  + GTG++  +PGH   DY    K GLP+++ 
Sbjct: 607  GSDLENCRYTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSP 666

Query: 485  MNKDGTLNKVAGLYCGLDRF-EARKKLWADLEETGLAVKKEAHTLRVPRSQRGGEIIEPL 544
            ++ +G   + AG + GL    E    + + L+E    V +E++  + P   R  +     
Sbjct: 667  VDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFR 726

Query: 545  VSKQWFVTMEPLAEKALRAVERGDLTIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPV 604
             ++QWF ++E      + A+   ++  +P +     +   S+  DWCISRQ  WG  IP 
Sbjct: 727  ATEQWFASVEGFRTATMDAI--NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPA 786

Query: 605  WY-IVGKNP---EEDY-----IVARNADEAL------EQARKKY-GKDVEIYQDPDVLDT 664
            +Y +  K P   EE       I+++   +A       +   +KY  K  +  +  D +D 
Sbjct: 787  FYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDV 846

Query: 665  WFSSALWPFSTLGWPDETAEDFKRFYPTTMLETGHDILFFWVARMVMLGIEFTGTVPFSY 724
            WF S         W     +     +P  +   G D    W    ++  I   G  P+S 
Sbjct: 847  WFDSG------SSWAGVLGKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSA 906

Query: 725  IYLHGLIRDSQGRKMSKTLGNVIDPLDTIK---------EFGTDALRFTLALGTAGQDLN 784
            +  HG + D +G KMSK+LGNV+DP   I+          +G D +R  ++      D+ 
Sbjct: 907  VITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVL 966

Query: 785  LSTERLTSNKAFTNKLWNAGKFILQNLPSQNDSQSWESIRSFEFEKEDCLLKLPLPECWI 836
            +  + L        KL    +++L NL        W    +  ++       LP+ +   
Sbjct: 967  IGPQILRQMSDIYRKLRGTLRYLLGNL------HDWRVDNAVPYQ------DLPIIDQHA 1026