Lcy02g001610 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g001610
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMannose-6-phosphate isomerase
LocationChr02: 2154940 .. 2158376 (-)
RNA-Seq ExpressionLcy02g001610
SyntenyLcy02g001610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTATACCCCTTCAACTTTCTCTAGAATTCCCACATCTCCCTTTTCTTCTTCGTCTTCTTCCTCTTCCTCTCCCTCCTCCTCTTTCCCTCTGCAATTTTCATGGACTCCGATGCTGTTTCCGCCCTCAAACACACCAATGTCCTCCGATTGAAGTGCTCCGTCCAGAATTACGATTGGGGAATCAGAGGACGCGATTCCCACGTCGCCCGTCTCTTCGCCTTCAACTCTGGATCGGTCGTCGATTCCGATAAACCCTACGCCGAGTTCTGGATGGGGACTCACGATTCCGGACCGTCGTTTTTTATCGCCGCCGCCGGGAATGGCGGCTCGGTTGCCTCCGATTCCACTTCCTTGAAGTCGTGGGTTTCGGAAAACCCTAATGTCCTCGGCGGTAAGGTTGTTGAGAAATGGGGCGCCGATATCCCCTTTCTGTTCAAGGTACGAAAACCGTGTTGAATTGTTCAAACGGATGATTTCGTTTCGTTTCGTTTTTCTTTCTCATTTCGATTCTCGGTTTTAGGTTCTCTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAAGGTGCTTCATAAGCTGGAGCCTAAGGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCACTGGCCATCACGGAGTTCGAGGCTCTGTGTGGATTCATCAGTCTCGAGGTAAATTAATTACTGGGAACCTCAAATCTTCCAGATGATCTGGGAATTATTAAACTGCCATGGATTTGTTTGTTTGTTTGTTTGTTTAAGTTTATGATAATTTCCTGTTTACCAGATTTTCTGGAAGCTTTGAATGATTATTTTAATGGCTCCCCTTGATTTTGTCTTCTTTAGTTAAATTTCAATTTTCTAAAAGTTCGAATGGAATGTTAACTGATTTCCTAATCTTGATCTTTACTATTCTTAGTTTAATGAATATTTATTCACTGGGATCTATTAAACTATTGTATTGTAAAGGTTTCTGTCGTCAGGCTTATATGTTCAATCTTCTTTATACCTTTTTTTTTTCCCTTCTTTGACAGAACCTTCTCTTAGACTTGTTAATCTTGATATGACAATTAACAAAATATTTTTTGTGCGTTGGTAACAGTCGAATTCGTTGCTTCTGGTCTAATAGTGAAATGTCTCATATGTCCTTAATTTGTGGTTTGATTTATTTTGTTCCCTTTAGCAGCAAGTTGCTTTAACTTTTAGTATGACTTTTCAGATGTGTCGGTGATAAAGGAAAATTGGGGTGAAAAAAATGCTGGCAATTGATGCTTAATGTATTCCACATGATTTGCAAACTTTTTTGGAAGTTAGTGCTTAATATGTTCCACGTTATTTGCAAACTTTTTGGAATATCTGACAAGATTTAAACAGGGGAAGACAGGTTCTTGTGAAACAAATAGTTCTTTTTTCTGGGTTTGAGATTTTTCTTTTGTTTCTCTGGTTTCTCAGCTGCTGTTTTTTCTTGAAAGACAGTCCATGGTTTTTCTTCCCATATTGATAAGAAATTTTGTTCTTTTTGTCATGTTTAGATGACACCATCTGTTGTTTTCTTTTCTTTCCAGGAGCTGAAGGATGTTCTTCGTACTGTTCCTGAGATTGTAGAAATGATTGGTGATGCAGACTCAAACAGAGTGTTGTTGTTAGATAATGAAGATGAAGAGGAGGACCTGAAGTCTGCTCTGAGGTCGGCTTTCACTCTGTTAATGTCGGCTACCAAATTGTTGATATCAAGAGCCATATCTGACTTGAAGAGGCGACTGCACTCTGAAAGTGAGGTAACTATGCAATGTTTCATTACGTTTTCTGTGGAAGCTTTCTGCTTCAATGATCCATTCTTTTTGCCTGTAGAATGTAGGAATCAAAGTTTATCTCAGGCCATGACTTTTCATTCTCTGTTACAGCAGCAATGAAGAACTTATATTTGTATCTGCTCTGTAGTCGTAGTATTGTTTTCTTTGAAAAAAGATAACAAAGAACCTCTGCTTTAACCTTTTATTTTTCTGTTTTATTTCTGCTCTATGTAGAGTGTGATTGTGAACCATGACACTGTGAATGTAATTTCTAGATCTTATCTTGATTTCTTTCTAGATTTGAACTGCCTGTTTTGAAGTACACTTCTTGATAGAGCTCTGTAACCACTTGTTTTCTGTTTGAAGTTCCCTCAATCCTTCTGTATCTGCCTTGTAGTTCATGTTTACACTTGCTCCTGGACTTCTGGAGTAACATACATTTTTGTGATCTGTGCTCATCTAGCTGCCTTGTTTTGTTTATTTAATCCTTCTTCAGGTGAGGCAACTAACTGCTAAGGAACTGTTGGTTTTACGTTTGGAGAAGCAATATCCAGATGATGTTGGCGTCATAGCAGCCTTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTTTACCTTGGGGCAAATGAGCCACATGCATATATAAGCGGTGAGTGCATCGAGTGCATGGCAACGTCTGATAACGTCGTTCGGGCTGGCCTAACTCCCAAGTTCCGTGATGTGCAGACTCTCTGTGCCATGCTTACTTACAAACAGGTAAACTTTTGCATTAAACCATTCCAATTGTTTCTCTTGTAAGTTTGATATCAAGTTCAAGAAACAATATGACGTTCTTGGATGTTCATCCATCAACAACCGAAAAAATTTTACAGTTGCGCGAAAAATACGAGAAGGCCAGAGTTTAAAAGTTCTGTTGTATTTATATGCTTTGATCTGCAGGGCTTTCCTGAAATCCTCCGAGGAGTCCCCATGAATCCATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGTTGTGTTCTTCCCAAGGGAGCATCCGTTTTATTTCCCCCGAGCCCCGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGATGTTGCTGCATGAAGAATCACTGAGAAGTGATGTAATTAGTGAAGGAGACGTCCTCTTTGTACCTGCTCAAACTGAGATTAATATAACAAGTGAAGTTGAGTTGCTCATTTTTAGAACAGGAGTAAACAACAAGTTCTTTGACACATTCATGAATGGTAAATAAAATATTAACCTTGATTTTTTCTTTCTTTCTTTTTTACCCTTTTTTAGATGGTTTCTTATCATGATGTATATTTTTTTTCATTAACTTGTATATGAGAACTACATGGTTCTGTTGCTAAAATTCTTATTTGTTTATTTGTTCTCAATGATAATACAAACATAGTTATGTGCAATAATCTCAATTCAAAGATTGTTTAGTGGATGATCTTCCACACTCCTACATTTGTTGAACTAAAAGACCATTAGGATTACTTTATGAGGCAATGTAGGAACTTATCAACCAAATATATCATTAACAAGTTAGCAATTTTAAATAAGAGAATCACACTTGATTTGTAGCGATTTGGC

mRNA sequence

ATTTATACCCCTTCAACTTTCTCTAGAATTCCCACATCTCCCTTTTCTTCTTCGTCTTCTTCCTCTTCCTCTCCCTCCTCCTCTTTCCCTCTGCAATTTTCATGGACTCCGATGCTGTTTCCGCCCTCAAACACACCAATGTCCTCCGATTGAAGTGCTCCGTCCAGAATTACGATTGGGGAATCAGAGGACGCGATTCCCACGTCGCCCGTCTCTTCGCCTTCAACTCTGGATCGGTCGTCGATTCCGATAAACCCTACGCCGAGTTCTGGATGGGGACTCACGATTCCGGACCGTCGTTTTTTATCGCCGCCGCCGGGAATGGCGGCTCGGTTGCCTCCGATTCCACTTCCTTGAAGTCGTGGGTTTCGGAAAACCCTAATGTCCTCGGCGGTAAGGTTGTTGAGAAATGGGGCGCCGATATCCCCTTTCTGTTCAAGGTTCTCTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAAGGTGCTTCATAAGCTGGAGCCTAAGGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCACTGGCCATCACGGAGTTCGAGGCTCTGTGTGGATTCATCAGTCTCGAGGAGCTGAAGGATGTTCTTCGTACTGTTCCTGAGATTGTAGAAATGATTGGTGATGCAGACTCAAACAGAGTGTTGTTGTTAGATAATGAAGATGAAGAGGAGGACCTGAAGTCTGCTCTGAGGTCGGCTTTCACTCTGTTAATGTCGGCTACCAAATTGTTGATATCAAGAGCCATATCTGACTTGAAGAGGCGACTGCACTCTGAAAGTGAGGTGAGGCAACTAACTGCTAAGGAACTGTTGGTTTTACGTTTGGAGAAGCAATATCCAGATGATGTTGGCGTCATAGCAGCCTTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTTTACCTTGGGGCAAATGAGCCACATGCATATATAAGCGGTGAGTGCATCGAGTGCATGGCAACGTCTGATAACGTCGTTCGGGCTGGCCTAACTCCCAAGTTCCGTGATGTGCAGACTCTCTGTGCCATGCTTACTTACAAACAGGGCTTTCCTGAAATCCTCCGAGGAGTCCCCATGAATCCATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGTTGTGTTCTTCCCAAGGGAGCATCCGTTTTATTTCCCCCGAGCCCCGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGATGTTGCTGCATGAAGAATCACTGAGAAGTGATGTAATTAGTGAAGGAGACGTCCTCTTTGTACCTGCTCAAACTGAGATTAATATAACAAGTGAAGTTGAGTTGCTCATTTTTAGAACAGGAGTAAACAACAAGTTCTTTGACACATTCATGAATGGTAAATAAAATATTAACCTTGATTTTTTCTTTCTTTCTTTTTTACCCTTTTTTAGATGGTTTCTTATCATGATGTATATTTTTTTTCATTAACTTGTATATGAGAACTACATGGTTCTGTTGCTAAAATTCTTATTTGTTTATTTGTTCTCAATGATAATACAAACATAGTTATGTGCAATAATCTCAATTCAAAGATTGTTTAGTGGATGATCTTCCACACTCCTACATTTGTTGAACTAAAAGACCATTAGGATTACTTTATGAGGCAATGTAGGAACTTATCAACCAAATATATCATTAACAAGTTAGCAATTTTAAATAAGAGAATCACACTTGATTTGTAGCGATTTGGC

Coding sequence (CDS)

ATGGACTCCGATGCTGTTTCCGCCCTCAAACACACCAATGTCCTCCGATTGAAGTGCTCCGTCCAGAATTACGATTGGGGAATCAGAGGACGCGATTCCCACGTCGCCCGTCTCTTCGCCTTCAACTCTGGATCGGTCGTCGATTCCGATAAACCCTACGCCGAGTTCTGGATGGGGACTCACGATTCCGGACCGTCGTTTTTTATCGCCGCCGCCGGGAATGGCGGCTCGGTTGCCTCCGATTCCACTTCCTTGAAGTCGTGGGTTTCGGAAAACCCTAATGTCCTCGGCGGTAAGGTTGTTGAGAAATGGGGCGCCGATATCCCCTTTCTGTTCAAGGTTCTCTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAAGGTGCTTCATAAGCTGGAGCCTAAGGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCACTGGCCATCACGGAGTTCGAGGCTCTGTGTGGATTCATCAGTCTCGAGGAGCTGAAGGATGTTCTTCGTACTGTTCCTGAGATTGTAGAAATGATTGGTGATGCAGACTCAAACAGAGTGTTGTTGTTAGATAATGAAGATGAAGAGGAGGACCTGAAGTCTGCTCTGAGGTCGGCTTTCACTCTGTTAATGTCGGCTACCAAATTGTTGATATCAAGAGCCATATCTGACTTGAAGAGGCGACTGCACTCTGAAAGTGAGGTGAGGCAACTAACTGCTAAGGAACTGTTGGTTTTACGTTTGGAGAAGCAATATCCAGATGATGTTGGCGTCATAGCAGCCTTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTTTACCTTGGGGCAAATGAGCCACATGCATATATAAGCGGTGAGTGCATCGAGTGCATGGCAACGTCTGATAACGTCGTTCGGGCTGGCCTAACTCCCAAGTTCCGTGATGTGCAGACTCTCTGTGCCATGCTTACTTACAAACAGGGCTTTCCTGAAATCCTCCGAGGAGTCCCCATGAATCCATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGTTGTGTTCTTCCCAAGGGAGCATCCGTTTTATTTCCCCCGAGCCCCGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGATGTTGCTGCATGAAGAATCACTGAGAAGTGATGTAATTAGTGAAGGAGACGTCCTCTTTGTACCTGCTCAAACTGAGATTAATATAACAAGTGAAGTTGAGTTGCTCATTTTTAGAACAGGAGTAAACAACAAGTTCTTTGACACATTCATGAATGGTAAATAA

Protein sequence

MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGTHDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKPLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPEIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESEVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCVLPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVELLIFRTGVNNKFFDTFMNGK
Homology
BLAST of Lcy02g001610 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.8e-147
Identity = 257/416 (61.78%), Postives = 323/416 (77.64%), Query Frame = 0

Query: 16  RLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGTHDSGPSFFIAAAGNG 75
           RL+CSV++YDWG  G DS V R++A NS   +D  +PYAE WMGTH+SGPS+   A G+ 
Sbjct: 22  RLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADGSN 81

Query: 76  GSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKPLSIQAHPDKELAKVL 135
           G       +L+SW++ENP  LG +V+EKWG D+PFLFKVLSVA+PLSIQAHPDK+LAK +
Sbjct: 82  G------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLAKKM 141

Query: 136 HKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPEIVEMIGDADSNRVLL 195
           HK  P +Y+D NHKPEMALA T+FEALCGFI L+ELK V+R +PEI E++G  ++N+V  
Sbjct: 142 HKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQVFC 201

Query: 196 LDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESEVRQLTAKELLVLRLE 255
           +   DEE+ +KS +R+ FTLLMSA      + +S LKRRLH ES+ RQLT KE LVL+LE
Sbjct: 202 ITEHDEEK-VKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLVLKLE 261

Query: 256 KQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKF 315
           KQYP+D+GVI+AFF NYVKLNPGEALYLGANEPHAY+ GEC+E MATSDNVVRAGLT K 
Sbjct: 262 KQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGLTSKP 321

Query: 316 RDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCVLPKGASVLFPPSPGP 375
            D+QTLC+ML+YK G+PEIL+G  + PYIT+Y+PPF+EFEVD C LP GAS +FP  PGP
Sbjct: 322 LDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPSVPGP 381

Query: 376 SIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVELLIFRTGVNNKF 432
           S+ LV  GEG +  E S  +D IS GDVLFVPA TEI++ S  +L ++R G+N++F
Sbjct: 382 SLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGINSRF 428

BLAST of Lcy02g001610 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 252/443 (56.88%), Postives = 329/443 (74.27%), Query Frame = 0

Query: 1   MDSDAVSALKH--------TNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKP 60
           M +DA+    H          + RL+C V+NY+WG  G +S VARL   N+G  VDS+ P
Sbjct: 1   MGADAIQTNGHDQAKLTGGEEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIP 60

Query: 61  YAEFWMGTHDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLF 120
           YAEFWMGTH+SGPS     +G+G S      +LKSWV +NPN+LG KVV+KWG D+PFLF
Sbjct: 61  YAEFWMGTHESGPSHVEFGSGHGVS-DKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLF 120

Query: 121 KVLSVAKPLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELK 180
           KVLSV K LSIQAHP+K LA+ LH+ +P +YRD NHKPE+ALA+T F+ALCGF++L+ELK
Sbjct: 121 KVLSVTKALSIQAHPNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELK 180

Query: 181 DVLRTVPEIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLK 240
           +V+  VPEI E++G   ++++  +   DE+E +KS +R  FT LMSA+     + +S +K
Sbjct: 181 EVITNVPEITELVGSKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMK 240

Query: 241 RRLHSESEVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYI 300
            RL  E++ R+L+ KE LVL LEKQY  D+GVI+AFF NYVKLNPGEALYL ANEPHAYI
Sbjct: 241 NRLLLETKHRELSEKEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYI 300

Query: 301 SGECIECMATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFD 360
           SG+C+ECMA SDNVVRAGLTPK RDVQTLC+MLTYK G+PEIL+G P+ PY+T+Y+PPFD
Sbjct: 301 SGDCVECMAASDNVVRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFD 360

Query: 361 EFEVDRCVLPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEI 420
           EFEVD C LP+G S +FP  PGPS++LV  G+G L    S    +++ GDVLFVPA  EI
Sbjct: 361 EFEVDHCDLPRGKSTVFPAVPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEI 420

Query: 421 NITSEVELL-IFRTGVNNKFFDT 435
           ++T E +++ ++R GV+++FF T
Sbjct: 421 HVTGESDVMKLYRAGVSSRFFQT 440

BLAST of Lcy02g001610 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.4e-80
Identity = 188/445 (42.25%), Postives = 259/445 (58.20%), Query Frame = 0

Query: 11  HTNVLRLKCSVQNYDWGIRGRDSHVARL---FAFNSGSVVDSDKPYAEFWMGTHDSGPSF 70
           + N++ LKC  QNY+WG  G +S VA+L   +A     ++    PYAE WMG H S PS 
Sbjct: 18  NNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAPSK 77

Query: 71  F------IAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEK-WGADIPFLFKVLSVAKP 130
                  +       +V  +     S  S + + + G++VEK +G D PFLFKVLS+   
Sbjct: 78  VEYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDFPFLFKVLSIRTA 137

Query: 131 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 190
           LSIQAHPD +LA+VL K  P +Y+D  HKPE+A+A T FEALC F  L E++  + T+PE
Sbjct: 138 LSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLSEIQSFIDTIPE 197

Query: 191 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 250
               +     N ++ LD      D K  L+S  T L+ A  LLIS  + +L  RL+ + E
Sbjct: 198 FKNSL----PNNLIKLD------DCKEYLKSIVTSLLKADGLLISNNLKELNNRLNEKRE 257

Query: 251 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 310
             +    + LVL+L  QYP DVGV  A+ LNY+ L PGEAL+LGA EPHAYISG+C+ECM
Sbjct: 258 -EERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPHAYISGDCVECM 317

Query: 311 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVP-MNPYITK-----YVPPFDEF 370
           A SDNVVRAGLTPK +DV TL  MLTY+ G P+++  VP   P ++      Y PP DEF
Sbjct: 318 APSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLV--VPQQRPDLSLTNYRCYQPPVDEF 377

Query: 371 EVDRCVLPKGA-SVLFPPSPGPSIFLVAGGEGMLLHEESLRSDV--ISEGDVLFVPAQTE 430
           +V+   L     ++    S GPSI LV  G  + +  +S +S +  +  G +LFVPA T+
Sbjct: 378 QVEYYHLDDCCKNINVYSSKGPSIVLVYSG-NLSIENKSNQSTLNNLHTGSILFVPANTD 437

Query: 431 ---INITSEVELLIFRTGVNNKFFD 434
              I   S + + I+   V+N+ F+
Sbjct: 438 YQFIQSDSSIPVSIYVASVSNRIFN 448

BLAST of Lcy02g001610 vs. ExPASy Swiss-Prot
Match: Q8HXX2 (Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3)

HSP 1 Score: 293.1 bits (749), Expect = 5.2e-78
Identity = 176/425 (41.41%), Postives = 250/425 (58.82%), Query Frame = 0

Query: 14  VLRLKCSVQNYDWGIRGRDSHVARLFAFNSG-SVVDSDKPYAEFWMGTHDSGPSFFIAAA 73
           V  L C+VQ Y WG  G +S VARL A +   + +  DKPYAE WMGTH  G +      
Sbjct: 6   VFPLSCAVQQYAWGKMGYNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIF--- 65

Query: 74  GNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKPLSIQAHPDKELA 133
            NG S      +L  W++EN + LG KV + +  ++PFLFKVLSV  PLSIQAHP+KELA
Sbjct: 66  DNGIS----QKTLGQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELA 125

Query: 134 KVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPEIVEMIGDADSNR 193
           + LH   P+ Y DANHKPEMA+A+T F+ LCGF  +EE+   L+ VPE   +IGD  +  
Sbjct: 126 EKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATH 185

Query: 194 VLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSE----SEVRQLTAKE 253
           +    + D +  + SAL+S F+ LM + K +++  ++ L +R+  +    + +  +  + 
Sbjct: 186 LKQTMSHDSQA-VASALQSCFSHLMKSEKKVVAEQLNLLVKRISQQVAAGNNMEDIFGE- 245

Query: 254 LLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVR 313
            L+L+L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN VR
Sbjct: 246 -LLLQLHQQYPGDIGCFAIYFLNLLNLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVR 305

Query: 314 AGLTPKFRDVQTLCAMLTYKQGFPEILRGVPM----NPYITKYVPPFDEFEVDRCVLPKG 373
           AGLTPKF DV TLC ML+Y     +    +P     +PY++ Y PP  +F + +  +P  
Sbjct: 306 AGLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFAIMKMEVPGS 365

Query: 374 AS---VLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEIN--ITSEVE 425
            +   VL   S   SI LV  G  +     +     +  G VLF+ A   ++  +T   +
Sbjct: 366 VTEYKVLALDS--ASILLVVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKD 418

BLAST of Lcy02g001610 vs. ExPASy Swiss-Prot
Match: A5A6K3 (Mannose-6-phosphate isomerase OS=Pan troglodytes OX=9598 GN=MPI PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.5e-77
Identity = 172/423 (40.66%), Postives = 244/423 (57.68%), Query Frame = 0

Query: 14  VLRLKCSVQNYDWGIRGRDSHVARLFAFNSG-SVVDSDKPYAEFWMGTHDSGPSFFIAAA 73
           V  L C+VQ Y WG  G +S VARL A +   + +  DKPYAE WMGTH  G +  +   
Sbjct: 6   VFPLSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIL--- 65

Query: 74  GNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKPLSIQAHPDKELA 133
                      +L  W++EN + LG KV + +  ++PFLFKVLSV  PLSIQAHP+KELA
Sbjct: 66  ----DNRISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELA 125

Query: 134 KVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPEIVEMIGDADSNR 193
           + LH   P+ Y DANHKPEMA+A+T F+ LCGF  +EE+   L+ VPE   +IGD  +  
Sbjct: 126 EKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATH 185

Query: 194 VLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESEVRQLTAKEL--L 253
           +    + D +  + S+L+S F+ LM + K ++   ++ L +R+  ++            L
Sbjct: 186 LKQTMSHDSQA-VASSLQSCFSHLMKSEKKVVVEQLNLLVKRISQQAAAGNNMEDIFGEL 245

Query: 254 VLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAG 313
           +L+L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN VRAG
Sbjct: 246 LLQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAG 305

Query: 314 LTPKFRDVQTLCAMLTYKQGFPEILRGVPM----NPYITKYVPPFDEFEVDRCVLP---K 373
           LTPKF DV TLC ML+Y     +    +P     +PY++ Y PP  +F + +  +P    
Sbjct: 306 LTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSREDPYLSIYDPPVPDFTIMKTEVPGSVT 365

Query: 374 GASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEIN--ITSEVELL 425
           G  VL   S   SI L+  G  +     +     +  G VLF+ A   ++  +T   +LL
Sbjct: 366 GYKVLALDS--ASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLL 418

BLAST of Lcy02g001610 vs. ExPASy TrEMBL
Match: A0A6J1J7Z8 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 385/439 (87.70%), Postives = 413/439 (94.08%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS +K +N+ RLKCSV+NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRMKQSNIFRLKCSVRNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF IAA  NGGSV SDSTSLKSWV ENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVLENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E++LKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDNLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +LHEES RSDVISEGDVLF+P + E+NITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFIPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. ExPASy TrEMBL
Match: A0A6J1F1M9 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111441358 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 386/439 (87.93%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS  K +N+ RLKCSV NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF I+A  NGGSV SDSTSLKSWVSENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLISATENGGSVDSDSTSLKSWVSENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVL TVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E+DLKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKY+PPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYIPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +LHEES RSDVISEGDVLFVP + EINITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEINITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. ExPASy TrEMBL
Match: A0A6J1CQ99 (Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.5e-216
Identity = 383/439 (87.24%), Postives = 405/439 (92.26%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+S+AV   K +NV RLKCSVQNYDWGIRG DS VARLFA NSGS+VDSDKPYAEFWMGT
Sbjct: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF I AA NGG+V SDSTSLKSWVSENPN LG KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK+LHKL+PK YRDANHKPEMALAITEFEALCGFISLEELKDVLR+VPE
Sbjct: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
            VEMIG  D+NRVLLLD+EDEEEDLKS LRSAFT LMSATK +IS  ISDLKRRLHSESE
Sbjct: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM
Sbjct: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNVVRAGLTPKFRDVQTLCAMLTYK GFPEIL+GVP+NPYITKY+PPFDEFEVD CV
Sbjct: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP+GASVLFP SPGPSI LVA GEGM+L  ESL SDVISEGDVLFV A+TEI+ITSEVEL
Sbjct: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN++FFD FMNGK
Sbjct: 421 LIFRTGVNSRFFDAFMNGK 439

BLAST of Lcy02g001610 vs. ExPASy TrEMBL
Match: A0A5D3BQZ9 (Mannose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G002180 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 378/441 (85.71%), Postives = 401/441 (90.93%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDA    K +NV RLKCSVQ YDWGIRG+DS VARLFA NSGSV+D DKPYAEFWMGT
Sbjct: 1   MESDAFPTKKQSNVFRLKCSVQTYDWGIRGQDSLVARLFALNSGSVIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFI-AAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAK 120
           HDSGPSF I AA  NGG + S STSLKSWV ENPNVLG KVV+KWG+DIPFLFKVLSVAK
Sbjct: 61  HDSGPSFLIPAAVENGGRIDSYSTSLKSWVLENPNVLGDKVVQKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVP 180
           PLSIQAHPDKELAK LH L+PKVYRDANHKPEMALAIT+FEALCGFISLEELK VL TVP
Sbjct: 121 PLSIQAHPDKELAKELHILQPKVYRDANHKPEMALAITDFEALCGFISLEELKYVLHTVP 180

Query: 181 EIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSES 240
           E VEMIG A +NR+LLLD+EDEEEDLKSALR AFT LMSA KLLIS+ IS+L RRLHSES
Sbjct: 181 ESVEMIGTAATNRLLLLDDEDEEEDLKSALRLAFTQLMSAPKLLISKVISELTRRLHSES 240

Query: 241 EVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIEC 300
           EVRQLTAKE LVL LEKQYPDDVG+IAAFFLNYVKL+ GEALYLGANEPHAYI GECIEC
Sbjct: 241 EVRQLTAKEALVLNLEKQYPDDVGIIAAFFLNYVKLSAGEALYLGANEPHAYIKGECIEC 300

Query: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRC 360
           MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRC
Sbjct: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRC 360

Query: 361 VLPKGASVLFPPSPGPSIFLVAGGEG-MLLHEESLRSDVISEGDVLFVPAQTEINITSEV 420
            LPKG SVLFP SPGPSIFLVAGG+G M+L+EES+ SDVISEGDVLFVPA+TEIN+TSEV
Sbjct: 361 ALPKGTSVLFPSSPGPSIFLVAGGKGMMMLNEESVESDVISEGDVLFVPAETEINLTSEV 420

Query: 421 ELLIFRTGVNNKFFDTFMNGK 440
           ELLIFRTGVNNKFFDTFMNGK
Sbjct: 421 ELLIFRTGVNNKFFDTFMNGK 441

BLAST of Lcy02g001610 vs. ExPASy TrEMBL
Match: A0A1S3C581 (Mannose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103496801 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 378/441 (85.71%), Postives = 401/441 (90.93%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDA    K +NV RLKCSVQ YDWGIRG+DS VARLFA NSGSV+D DKPYAEFWMGT
Sbjct: 1   MESDAFPTKKQSNVFRLKCSVQTYDWGIRGQDSLVARLFALNSGSVIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFI-AAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAK 120
           HDSGPSF I AA  NGG + S STSLKSWV ENPNVLG KVV+KWG+DIPFLFKVLSVAK
Sbjct: 61  HDSGPSFLIPAAVENGGRIDSYSTSLKSWVLENPNVLGDKVVQKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVP 180
           PLSIQAHPDKELAK LH L+PKVYRDANHKPEMALAIT+FEALCGFISLEELK VL TVP
Sbjct: 121 PLSIQAHPDKELAKELHILQPKVYRDANHKPEMALAITDFEALCGFISLEELKYVLHTVP 180

Query: 181 EIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSES 240
           E VEMIG A +NR+LLLD+EDEEEDLKSALR AFT LMSA KLLIS+ IS+L RRLHSES
Sbjct: 181 ESVEMIGTAATNRLLLLDDEDEEEDLKSALRLAFTQLMSAPKLLISKVISELTRRLHSES 240

Query: 241 EVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIEC 300
           EVRQLTAKE LVL LEKQYPDDVG+IAAFFLNYVKL+ GEALYLGANEPHAYI GECIEC
Sbjct: 241 EVRQLTAKEALVLNLEKQYPDDVGIIAAFFLNYVKLSAGEALYLGANEPHAYIKGECIEC 300

Query: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRC 360
           MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRC
Sbjct: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRC 360

Query: 361 VLPKGASVLFPPSPGPSIFLVAGGEG-MLLHEESLRSDVISEGDVLFVPAQTEINITSEV 420
            LPKG SVLFP SPGPSIFLVAGG+G M+L+EES+ SDVISEGDVLFVPA+TEIN+TSEV
Sbjct: 361 ALPKGTSVLFPSSPGPSIFLVAGGKGMMMLNEESVESDVISEGDVLFVPAETEINLTSEV 420

Query: 421 ELLIFRTGVNNKFFDTFMNGK 440
           ELLIFRTGVNNKFFDTFMNGK
Sbjct: 421 ELLIFRTGVNNKFFDTFMNGK 441

BLAST of Lcy02g001610 vs. NCBI nr
Match: KAG6581183.1 (Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.3 bits (2006), Expect = 6.9e-221
Identity = 387/439 (88.15%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS  K +N+ RLKCSV NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF IAA  NGGSV SDSTSLKSWVSENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVSENPNVLGDKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVL TVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E+DLKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +LHEES RSDVISEGDVLFVP + E+NITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. NCBI nr
Match: XP_038875067.1 (mannose-6-phosphate isomerase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 775.8 bits (2002), Expect = 2.0e-220
Identity = 389/441 (88.21%), Postives = 414/441 (93.88%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDA S  K +NVLRLKCSVQ YDWGIRG+DS VAR FA NSGS++DSDKPYAEFWMGT
Sbjct: 1   MESDAFSMKKQSNVLRLKCSVQTYDWGIRGQDSRVARFFALNSGSIIDSDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAG-NGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAK 120
           HDSGPSF I AAG NGG + SDSTSLKSWV ENPNVLG KVV+KWG+DIPFLFKVLSVAK
Sbjct: 61  HDSGPSFLIPAAGENGGGIDSDSTSLKSWVLENPNVLGDKVVQKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVP 180
           PLSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVL TVP
Sbjct: 121 PLSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVP 180

Query: 181 EIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSES 240
           EIVEMIG+A +NRVLLLD+ED+EEDLKSALRSAFT LMSA+KL+IS+A S+LKRRLHS+S
Sbjct: 181 EIVEMIGNAATNRVLLLDDEDDEEDLKSALRSAFTQLMSASKLVISKATSELKRRLHSQS 240

Query: 241 EVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIEC 300
           EVRQLTAKE+LVL LEKQYPDDVGVIAAFFLNYVKL+PGEALYLGANEPHAYI GECIEC
Sbjct: 241 EVRQLTAKEVLVLSLEKQYPDDVGVIAAFFLNYVKLSPGEALYLGANEPHAYIRGECIEC 300

Query: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRC 360
           MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKY+PPFDEFE+DRC
Sbjct: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYIPPFDEFEIDRC 360

Query: 361 VLPKGASVLFPPSPGPSIFLVAGGEG-MLLHEESLRSDVISEGDVLFVPAQTEINITSEV 420
           VLPKG SVLFP SPGPSIFLVAGGEG MLL+EES RSDV+SEGDVLFVPAQTEINITSEV
Sbjct: 361 VLPKGESVLFPASPGPSIFLVAGGEGMMLLNEESHRSDVVSEGDVLFVPAQTEINITSEV 420

Query: 421 ELLIFRTGVNNKFFDTFMNGK 440
           ELLIFRTGVNNKFFDTFMNGK
Sbjct: 421 ELLIFRTGVNNKFFDTFMNGK 441

BLAST of Lcy02g001610 vs. NCBI nr
Match: XP_022934077.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 775.4 bits (2001), Expect = 2.6e-220
Identity = 386/439 (87.93%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS  K +N+ RLKCSV NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF I+A  NGGSV SDSTSLKSWVSENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLISATENGGSVDSDSTSLKSWVSENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVL TVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E+DLKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKY+PPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYIPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +LHEES RSDVISEGDVLFVP + EINITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEINITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. NCBI nr
Match: XP_022983634.1 (mannose-6-phosphate isomerase 1-like [Cucurbita maxima])

HSP 1 Score: 775.4 bits (2001), Expect = 2.6e-220
Identity = 385/439 (87.70%), Postives = 413/439 (94.08%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS +K +N+ RLKCSV+NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRMKQSNIFRLKCSVRNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF IAA  NGGSV SDSTSLKSWV ENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVLENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E++LKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDNLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +LHEES RSDVISEGDVLF+P + E+NITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFIPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. NCBI nr
Match: KAG7017923.1 (Mannose-6-phosphate isomerase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 774.2 bits (1998), Expect = 5.8e-220
Identity = 386/439 (87.93%), Postives = 410/439 (93.39%), Query Frame = 0

Query: 1   MDSDAVSALKHTNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGT 60
           M+SDAVS  K +N+ RLKCSV NYDWGIRG DS VARLFA NSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKP 120
           HDSGPSF IAA  NGGSV SDSTSLKSWVSENPNVLG KVVEKWGADIPFLFKVLSVAKP
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVSENPNVLGDKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180
           LSIQAHPDKELAK LH L+PKVYRDANHKPEMALAITEFEALCGFISLEELKDVL TVPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 IVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESE 240
           IVEMIGDAD+NRVLLL++ED+E+DLKSALRSAFT LMSA+K LIS+AIS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYKQGFPEIL+GVPMNPYITKYVPPFDEFEVDRCV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVEL 420
           LP GASV+FP SPGPSIFLVAGGEG +L EES RSDVISEGDVLFVP + E+NITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLQEESHRSDVISEGDVLFVPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNNKFFDTFMNGK 440
           LIFRTGVN+KFFDTFMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of Lcy02g001610 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-148
Identity = 257/416 (61.78%), Postives = 323/416 (77.64%), Query Frame = 0

Query: 16  RLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKPYAEFWMGTHDSGPSFFIAAAGNG 75
           RL+CSV++YDWG  G DS V R++A NS   +D  +PYAE WMGTH+SGPS+   A G+ 
Sbjct: 22  RLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADGSN 81

Query: 76  GSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLFKVLSVAKPLSIQAHPDKELAKVL 135
           G       +L+SW++ENP  LG +V+EKWG D+PFLFKVLSVA+PLSIQAHPDK+LAK +
Sbjct: 82  G------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLAKKM 141

Query: 136 HKLEPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPEIVEMIGDADSNRVLL 195
           HK  P +Y+D NHKPEMALA T+FEALCGFI L+ELK V+R +PEI E++G  ++N+V  
Sbjct: 142 HKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQVFC 201

Query: 196 LDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLKRRLHSESEVRQLTAKELLVLRLE 255
           +   DEE+ +KS +R+ FTLLMSA      + +S LKRRLH ES+ RQLT KE LVL+LE
Sbjct: 202 ITEHDEEK-VKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLVLKLE 261

Query: 256 KQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKF 315
           KQYP+D+GVI+AFF NYVKLNPGEALYLGANEPHAY+ GEC+E MATSDNVVRAGLT K 
Sbjct: 262 KQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGLTSKP 321

Query: 316 RDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFDEFEVDRCVLPKGASVLFPPSPGP 375
            D+QTLC+ML+YK G+PEIL+G  + PYIT+Y+PPF+EFEVD C LP GAS +FP  PGP
Sbjct: 322 LDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPSVPGP 381

Query: 376 SIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEINITSEVELLIFRTGVNNKF 432
           S+ LV  GEG +  E S  +D IS GDVLFVPA TEI++ S  +L ++R G+N++F
Sbjct: 382 SLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGINSRF 428

BLAST of Lcy02g001610 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 252/443 (56.88%), Postives = 329/443 (74.27%), Query Frame = 0

Query: 1   MDSDAVSALKH--------TNVLRLKCSVQNYDWGIRGRDSHVARLFAFNSGSVVDSDKP 60
           M +DA+    H          + RL+C V+NY+WG  G +S VARL   N+G  VDS+ P
Sbjct: 1   MGADAIQTNGHDQAKLTGGEEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIP 60

Query: 61  YAEFWMGTHDSGPSFFIAAAGNGGSVASDSTSLKSWVSENPNVLGGKVVEKWGADIPFLF 120
           YAEFWMGTH+SGPS     +G+G S      +LKSWV +NPN+LG KVV+KWG D+PFLF
Sbjct: 61  YAEFWMGTHESGPSHVEFGSGHGVS-DKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLF 120

Query: 121 KVLSVAKPLSIQAHPDKELAKVLHKLEPKVYRDANHKPEMALAITEFEALCGFISLEELK 180
           KVLSV K LSIQAHP+K LA+ LH+ +P +YRD NHKPE+ALA+T F+ALCGF++L+ELK
Sbjct: 121 KVLSVTKALSIQAHPNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELK 180

Query: 181 DVLRTVPEIVEMIGDADSNRVLLLDNEDEEEDLKSALRSAFTLLMSATKLLISRAISDLK 240
           +V+  VPEI E++G   ++++  +   DE+E +KS +R  FT LMSA+     + +S +K
Sbjct: 181 EVITNVPEITELVGSKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMK 240

Query: 241 RRLHSESEVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYI 300
            RL  E++ R+L+ KE LVL LEKQY  D+GVI+AFF NYVKLNPGEALYL ANEPHAYI
Sbjct: 241 NRLLLETKHRELSEKEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYI 300

Query: 301 SGECIECMATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILRGVPMNPYITKYVPPFD 360
           SG+C+ECMA SDNVVRAGLTPK RDVQTLC+MLTYK G+PEIL+G P+ PY+T+Y+PPFD
Sbjct: 301 SGDCVECMAASDNVVRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFD 360

Query: 361 EFEVDRCVLPKGASVLFPPSPGPSIFLVAGGEGMLLHEESLRSDVISEGDVLFVPAQTEI 420
           EFEVD C LP+G S +FP  PGPS++LV  G+G L    S    +++ GDVLFVPA  EI
Sbjct: 361 EFEVDHCDLPRGKSTVFPAVPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEI 420

Query: 421 NITSEVELL-IFRTGVNNKFFDT 435
           ++T E +++ ++R GV+++FF T
Sbjct: 421 HVTGESDVMKLYRAGVSSRFFQT 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8841.8e-14761.78Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q9FZH51.7e-14256.88Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q54PA09.4e-8042.25Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Q8HXX25.2e-7841.41Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3[more]
A5A6K31.5e-7740.66Mannose-6-phosphate isomerase OS=Pan troglodytes OX=9598 GN=MPI PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J7Z81.3e-22087.70Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 S... [more]
A0A6J1F1M91.3e-22087.93Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111441358 PE=3... [more]
A0A6J1CQ992.5e-21687.24Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=... [more]
A0A5D3BQZ91.1e-21185.71Mannose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3C5811.1e-21185.71Mannose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103496801 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6581183.16.9e-22188.15Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038875067.12.0e-22088.21mannose-6-phosphate isomerase 1-like isoform X2 [Benincasa hispida][more]
XP_022934077.12.6e-22087.93mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
XP_022983634.12.6e-22087.70mannose-6-phosphate isomerase 1-like [Cucurbita maxima][more]
KAG7017923.15.8e-22087.93Mannose-6-phosphate isomerase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G02570.11.3e-14861.78Mannose-6-phosphate isomerase, type I [more]
AT1G67070.11.2e-14356.88Mannose-6-phosphate isomerase, type I [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 308..327
score: 62.0
coord: 17..35
score: 46.32
coord: 108..129
score: 73.64
coord: 143..166
score: 64.17
coord: 270..289
score: 59.5
coord: 254..269
score: 41.25
coord: 289..308
score: 70.0
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 11..432
e-value: 6.6E-127
score: 422.1
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 10..433
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 18..309
e-value: 5.1E-136
score: 456.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 14..424
e-value: 5.1E-136
score: 456.1
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 167..334
e-value: 5.1E-136
score: 456.1
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 22..425
e-value: 7.5E-123
score: 408.5
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 22..425
e-value: 7.5E-123
score: 408.5
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 10..433
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 17..327
e-value: 6.01066E-126
score: 363.023
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 15..422
e-value: 1.6E-78
score: 262.4
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 14..388
e-value: 9.1E-108
score: 360.8
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00966PMI_I_2coord: 289..314
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 143..151
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 13..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g001610.1Lcy02g001610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding