Lcy02g001360 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g001360
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr02: 1810920 .. 1811708 (+)
RNA-Seq ExpressionLcy02g001360
SyntenyLcy02g001360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTGGAGTTGGTGGAAGTTCAAGGACTTTTGGGATGGTGTTTCAAAAATTCTAGATGCCCAGGAAGCAGGTTTAGCTAGCCATATTATTTGGATAATTTGGCAGAAGAGGAACCAATTGAAACAAGCAAGCAGCAAACAAGATATGGCAACTCACCAATGATATTCTCAACGCAGCAGACAAATTCATATTTGAGAACGAGAATGAGAAATCTAACCAGGGCAAGAAGTCGGAGACCCTCCTGAGTCTCAGACGCTGGAATCCGCCGGGCAGATCTCAATGGAAGCTGAATGTGGATGCACTTGGTTTGAAGCATCAAATTCAGGCGGTTTGGGGTGGATTGCGCGAGACTTTGAAGGTTCTCTGATAGGCGCAGGCTGCAAGAAGATAAATAGAAGATTAGACATCAAAATTCTTGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAAAGGCTCAGGGTTTACCCGGAGAATTCGCTGCATGAGGTGGTGGTTGAGTCGGATGCTGCCGAAATCGTCAAGCTGATCATCGAAGAATCCGAAGACTTCTCGGAAATTTCCCTCTTGATCGACGAGATTCACGAGGTAGCGAATCGAATGACCCCTGTAACTTTTGTACATTGCCCTCGGGCTGCAAATTTTTTGGCGCATTCTCTGGCGTGCGGAGCTGTTAGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAATGGATGGTTGTGTTGTTGTAGGGGATGAGTTACCCCCTGGTTTGCCTCAGTTATGGAGGTAG

mRNA sequence

ATGTGCTGGAGTTGGTGGAAGTTCAAGGACTTTTGGGATGGTGTTTCAAAAATTCTAGATGCCCAGGAAGCAGGGCAAGAAGTCGGAGACCCTCCTGAGTCTCAGACGCTGGAATCCGCCGGGCAGATCTCAATGGAAGCTGAATGTGGATGCACTTGGTTTGAAGCATCAAATTCAGGCGGTTTGGGGTGGATTGCGCGAGACTTTGAAGGTTCTCTGATAGGCGCAGGCTGCAAGAAGATAAATAGAAGATTAGACATCAAAATTCTTGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAAAGGCTCAGGGTTTACCCGGAGAATTCGCTGCATGAGGTGGTGGTTGAGTCGGATGCTGCCGAAATCGTCAAGCTGATCATCGAAGAATCCGAAGACTTCTCGGAAATTTCCCTCTTGATCGACGAGATTCACGAGGTAGCGAATCGAATGACCCCTGTAACTTTTGTACATTGCCCTCGGGCTGCAAATTTTTTGGCGCATTCTCTGGCGTGCGGAGCTGTTAGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAATGGATGGTTGTGTTGTTGTAGGGGATGAGTTACCCCCTGGTTTGCCTCAGTTATGGAGGTAG

Coding sequence (CDS)

ATGTGCTGGAGTTGGTGGAAGTTCAAGGACTTTTGGGATGGTGTTTCAAAAATTCTAGATGCCCAGGAAGCAGGGCAAGAAGTCGGAGACCCTCCTGAGTCTCAGACGCTGGAATCCGCCGGGCAGATCTCAATGGAAGCTGAATGTGGATGCACTTGGTTTGAAGCATCAAATTCAGGCGGTTTGGGGTGGATTGCGCGAGACTTTGAAGGTTCTCTGATAGGCGCAGGCTGCAAGAAGATAAATAGAAGATTAGACATCAAAATTCTTGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAAAGGCTCAGGGTTTACCCGGAGAATTCGCTGCATGAGGTGGTGGTTGAGTCGGATGCTGCCGAAATCGTCAAGCTGATCATCGAAGAATCCGAAGACTTCTCGGAAATTTCCCTCTTGATCGACGAGATTCACGAGGTAGCGAATCGAATGACCCCTGTAACTTTTGTACATTGCCCTCGGGCTGCAAATTTTTTGGCGCATTCTCTGGCGTGCGGAGCTGTTAGAAGCTATAGCCATGGTCGAAGGTCTCTCACGTTTATCGGAATGGATGGTTGTGTTGTTGTAGGGGATGAGTTACCCCCTGGTTTGCCTCAGTTATGGAGGTAG

Protein sequence

MCWSWWKFKDFWDGVSKILDAQEAGQEVGDPPESQTLESAGQISMEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAHSLACGAVRSYSHGRRSLTFIGMDGCVVVGDELPPGLPQLWR
Homology
BLAST of Lcy02g001360 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.4e-10
Identity = 46/138 (33.33%), Postives = 74/138 (53.62%), Query Frame = 0

Query: 44  SMEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRL- 103
           S +      W   +N+GG+GWI RD +G +I A C+ I    +I  LE +A+ EGL  + 
Sbjct: 86  SWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIR 145

Query: 104 SERLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHC 163
            E  R   +     + +ESD+ E + L+  + +D +EI  L++EI ++   M  V+  H 
Sbjct: 146 QEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 205

Query: 164 PRAANFLAHSLACGAVRS 181
            R AN +AH LA  A+ +
Sbjct: 206 SREANKVAHDLARRAMEN 223

BLAST of Lcy02g001360 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 6.7e-10
Identity = 45/137 (32.85%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 44  SMEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLS 103
           S +      W   +N+GG+GWI RD +G +I A C+ I    +I  LE +A+ EG     
Sbjct: 86  SWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEG----- 145

Query: 104 ERLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCP 163
             LR   +     + +ESD+ E + L+  + +D +EI  L++EI ++   M  V+  H  
Sbjct: 146 --LRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 205

Query: 164 RAANFLAHSLACGAVRS 181
           R AN +AH LA  A+ +
Sbjct: 206 REANKVAHGLARRAMEN 215

BLAST of Lcy02g001360 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.8e-08
Identity = 39/123 (31.71%), Postives = 72/123 (58.54%), Query Frame = 0

Query: 52  TWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPE 111
           +W ++++ GG+GWI R ++G ++ AG + +    ++K+LEA A++EGL  L+    + P 
Sbjct: 86  SWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRP- 145

Query: 112 NSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAH 171
             LH   +E+D+AE+  L+  + ED ++   +++EI  + +    + F    R  N  AH
Sbjct: 146 --LH---IETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAH 202

Query: 172 SLA 175
           SLA
Sbjct: 206 SLA 202

BLAST of Lcy02g001360 vs. ExPASy TrEMBL
Match: A0A803MUM1 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.1e-07
Identity = 42/121 (34.71%), Postives = 67/121 (55.37%), Query Frame = 0

Query: 62  LGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPENSLHEVVVES 121
           +G + RD  G ++ AGC+++++  D  I+EA AMV G       L++  + ++  V+VES
Sbjct: 186 MGVVVRDHLGEVVRAGCQQVHQSWDASIVEAKAMVLG-------LKLAMQCNVKRVIVES 245

Query: 122 DAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAHSLACGAVRSY 181
           D   +VKL+    +D S + LL  EI  VA     + F H  R AN +AH++A  +   Y
Sbjct: 246 DCERLVKLVNGVGKDGSFLGLLAKEIKCVAEFFDSIVFAHVFREANSVAHAMAHLSPLEY 299

Query: 182 S 183
           S
Sbjct: 306 S 299

BLAST of Lcy02g001360 vs. ExPASy TrEMBL
Match: A0A251QFD7 (RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.1e-07
Identity = 36/136 (26.47%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 45  MEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSE 104
           ++  C  TW   +  GG GW+ RDF G ++ AG K+  R          A++  +  +  
Sbjct: 60  VKVNCDGTWNAQTMKGGYGWVIRDFVGWMLQAGGKRDRR-------YGSALIAEVDAIRA 119

Query: 105 RLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPR 164
            +    +     ++VESD+   ++++  +    +E+  L+ +IH V   +  VTF + PR
Sbjct: 120 AVVACQQGGFSHIIVESDSLSAIQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPR 179

Query: 165 AANFLAHSLACGAVRS 181
           + N  AH +A  A R+
Sbjct: 180 SCNMAAHEVAAFACRN 188

BLAST of Lcy02g001360 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-10
Identity = 46/138 (33.33%), Postives = 74/138 (53.62%), Query Frame = 0

Query: 44  SMEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRL- 103
           S +      W   +N+GG+GWI RD +G +I A C+ I    +I  LE +A+ EGL  + 
Sbjct: 86  SWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIR 145

Query: 104 SERLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHC 163
            E  R   +     + +ESD+ E + L+  + +D +EI  L++EI ++   M  V+  H 
Sbjct: 146 QEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 205

Query: 164 PRAANFLAHSLACGAVRS 181
            R AN +AH LA  A+ +
Sbjct: 206 SREANKVAHDLARRAMEN 223

BLAST of Lcy02g001360 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-09
Identity = 45/137 (32.85%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 44  SMEAECGCTWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLS 103
           S +      W   +N+GG+GWI RD +G +I A C+ I    +I  LE +A+ EG     
Sbjct: 86  SWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEG----- 145

Query: 104 ERLRVYPENSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCP 163
             LR   +     + +ESD+ E + L+  + +D +EI  L++EI ++   M  V+  H  
Sbjct: 146 --LRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 205

Query: 164 RAANFLAHSLACGAVRS 181
           R AN +AH LA  A+ +
Sbjct: 206 REANKVAHGLARRAMEN 215

BLAST of Lcy02g001360 vs. NCBI nr
Match: XP_037439691.1 (uncharacterized protein LOC119307722 isoform X1 [Triticum dicoccoides])

HSP 1 Score: 72.8 bits (177), Expect = 4.0e-09
Identity = 50/150 (33.33%), Postives = 83/150 (55.33%), Query Frame = 0

Query: 59  SGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPENSLHEVV 118
           +G  G + RD  G+ I A C      +D+  +EA+A++EGL RL+E L +Y       +V
Sbjct: 322 AGATGAVLRDSSGAFIAASCSFRQYAMDVPSMEALALLEGL-RLAEHLGIY------ALV 381

Query: 119 VESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAHSLACGAV 178
           VESD+ EIV+ + + SE  +  +++ D+  +V +  +  T VHC R +N +AH LA  + 
Sbjct: 382 VESDSQEIVQAMTDPSEYRASAAVVTDDCRQVLSSFSRATTVHCARESNGVAHWLARTSY 441

Query: 179 RSYSH--GRRSLTFIGMDGCVVVGDELPPG 207
           R   +  G  ++T + + G V V +  P G
Sbjct: 442 RRQLNEAGDMNVTKMLLMGDVDVNETDPEG 464

BLAST of Lcy02g001360 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-07
Identity = 39/123 (31.71%), Postives = 72/123 (58.54%), Query Frame = 0

Query: 52  TWFEASNSGGLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPE 111
           +W ++++ GG+GWI R ++G ++ AG + +    ++K+LEA A++EGL  L+    + P 
Sbjct: 86  SWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRP- 145

Query: 112 NSLHEVVVESDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAH 171
             LH   +E+D+AE+  L+  + ED ++   +++EI  + +    + F    R  N  AH
Sbjct: 146 --LH---IETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAH 202

Query: 172 SLA 175
           SLA
Sbjct: 206 SLA 202

BLAST of Lcy02g001360 vs. NCBI nr
Match: XP_021841246.1 (uncharacterized protein LOC110781352 [Spinacia oleracea])

HSP 1 Score: 66.6 bits (161), Expect = 2.9e-07
Identity = 43/146 (29.45%), Postives = 77/146 (52.74%), Query Frame = 0

Query: 61  GLGWIARDFEGSLIGAGCKKINRRLDIKILEAIAMVEGLSRLSERLRVYPENSLHEVVVE 120
           G+G I RD EG+++ A C ++  + ++ I+EA ++V G       L++  +    +VVVE
Sbjct: 99  GMGVIIRDEEGAVVRAACLQVAHQWEVSIVEAKSIVLG-------LKLAIQCGARDVVVE 158

Query: 121 SDAAEIVKLIIEESEDFSEISLLIDEIHEVANRMTPVTFVHCPRAANFLAHSLACG--AV 180
           SD  +++KL+  E  D   + ++  EI E+A+    + FVH  R AN   H++A      
Sbjct: 159 SDCLKVIKLVNGEIRDGLYLGMIGREIREIASLFDSINFVHVYREANLADHTMAMHEFCT 218

Query: 181 RSYSHGRRSLT--FIGMDGCVVVGDE 203
           R +  G  S+    +  D C++  D+
Sbjct: 219 RVWIRGCPSIVEDIVATDFCLINNDK 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DSV11.4e-1033.33uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1CP266.7e-1032.85uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DNV94.8e-0831.71uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A803MUM13.1e-0734.71Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A251QFD73.1e-0726.47RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE... [more]
Match NameE-valueIdentityDescription
XP_022156777.12.8e-1033.33uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022143535.11.4e-0932.85uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_037439691.14.0e-0933.33uncharacterized protein LOC119307722 isoform X1 [Triticum dicoccoides][more]
XP_022155262.11.0e-0731.71uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_021841246.12.9e-0729.45uncharacterized protein LOC110781352 [Spinacia oleracea][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 49..183
e-value: 2.8E-8
score: 35.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 53..174
e-value: 6.1E-18
score: 64.8
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 53..182
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 53..182
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 53..174
e-value: 4.55614E-15
score: 66.954
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 58..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g001360.1Lcy02g001360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity