Lcy02g000890 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g000890
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptioncytochrome P450 89A2-like
LocationChr02: 1244780 .. 1246701 (-)
RNA-Seq ExpressionLcy02g000890
SyntenyLcy02g000890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTTTGGGGACAAGCTGAATGAGACAGAGATCTTGGAAATTCAACAAGTGCAGCGTGAGATTTTGGTAAATCTTCAGCGTTTTGTTATACTCAATTTCTGGCCTAAGTTGATGAAGATTTTGCTTCGAAAGCGTTGGAAGGCTTACTTACAACTCAAAAGCAAGCAAGAAAAGGTTGACTTTCCGATACTTGAATTCAAAATCTTAAAGCTCATAAGTTAGGGTGTTTGGCATGGAAAAATTATTAGGTGAGGCCGCCACGATGAGAATGATAGTTTTGTATCGTTGGATGTAAAAAAAAAATGGTAAAAATACCTCGAGAGACAGAGGGACAAACATTAAGATTTACATCCAATGGTTTAAAAAGAGCATCCACATTGTGTTGACTTCATTGAATAATTTGTAGTTAACATGCCTCCTTATGTACGACTAAAAGTTCTTTTTTCATTTTTCTTTTAACCTAGCAATATCAGGTAAAATTTTTAATAAATTCTTGATAACTAAAGAGGGGTTTTGAGAAATTCTAAGTATAAATAAAAAGCGCTCGAAATTACTTTTAAATTAAGCATTTATTTAATTTTCTTCGAAAAGTACTTAAAAAGATATTTCACCGTAGGGATCTCCCTACACACCCCATTAAAATCTTGACAACTAAGAGAGAAAGAATGGAGAAAATGAGAAATTTTTTTATCTCTCCTAATTGTCAACATTTTAATGGTGATAATCACGGAGATCCCTACATATACCGAGTGTATGCACCCAAGTTTTTTCCTTTCTTTTAAAAACACTTTTTTTTTCTAAATATTATAACGGTTAGAATATGGGATCTTCGACTTTGATATCATATCAAGAGATCTGTGATTCGAATCCTTCACTCCACATGTTGTCAAACAAAAAAAAATTATTGAATTGCATTGTATTTAACCATTTATTAATTTTTTAATAGGTCCTAATCCCATTTATCAAGGCACGAGAGAAGGCAAAACAAGAAACATCAAAAAAAGATGGAGAAGAAAAAGAAGAATTTGTAGTATCATATGTGGATACTCTGCTCGATTTGCACCTTATCGATGAAAATAGAAAGCTTAAAAATGAAGAAATGGTGAGTTTATGCTCGGAGTTCCTCAACGCCGGGACTGACACCACTGCCACAACTTTGCAATGGATAATGGCAAATTTAGTAAAGTACCCACACATCCAACAAAAACTATTCCAAGAGATCAAACGAGTCATGGAATCCAACACAAAACAACATGGGTTACGGTAAATTTAATCGTTTTTTTTATTTTAATATTTCTTTTCACTCGTGCATCGATACGGAACTAGACGAAGAAATGATATTTTCAAAAATTAAAAATTAGACTCTCTACTCTCGACGTAAAAGTATAATTTGATAAGTTACGATACATTTAATTAGGGAGGAGGAGGTAAAGGAAGAAGATTTGGGGAAGCTTCCATATTTGAAAGCCGTGGTTCTAGAAGGATTACGAAGGCACCCGCCGGGACATTTCATGTTACCACATGCCGTGAAAGAAGATACGATGTTGGAGAATTATTTCATTCCCAAAAATGGAACGGTGAATATCTTGGCCGTGGAGATGGGGTGGGATCCGGAAGTATGGGAGGATCCGATGGCGTTTAAGCCAGAGAGATTCGTGAGCAGCGATGGAGAGGCGACGACGATGACGTTTGATGTGACATGTAGCAAGGAGATCAAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGGGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAGATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAACCCTCTCAAAGCCCATATACATCCAAGGTTTTAG

mRNA sequence

ATGTGCTTTGGGGACAAGCTGAATGAGACAGAGATCTTGGAAATTCAACAAGTGCAGCGTGAGATTTTGGTAAATCTTCAGCGTTTTGTTATACTCAATTTCTGGCCTAAGTTGATGAAGATTTTGCTTCGAAAGCGTTGGAAGGCTTACTTACAACTCAAAAGCAAGCAAGAAAAGGTCCTAATCCCATTTATCAAGGCACGAGAGAAGGCAAAACAAGAAACATCAAAAAAAGATGGAGAAGAAAAAGAAGAATTTGTAGTATCATATGTGGATACTCTGCTCGATTTGCACCTTATCGATGAAAATAGAAAGCTTAAAAATGAAGAAATGGTGAGTTTATGCTCGGAGTTCCTCAACGCCGGGACTGACACCACTGCCACAACTTTGCAATGGATAATGGCAAATTTAGTAAAGTACCCACACATCCAACAAAAACTATTCCAAGAGATCAAACGAGTCATGGAATCCAACACAAAACAACATGGGTTACGGGAGGAGGAGGTAAAGGAAGAAGATTTGGGGAAGCTTCCATATTTGAAAGCCGTGGTTCTAGAAGGATTACGAAGGCACCCGCCGGGACATTTCATGTTACCACATGCCGTGAAAGAAGATACGATGTTGGAGAATTATTTCATTCCCAAAAATGGAACGGTGAATATCTTGGCCGTGGAGATGGGGTGGGATCCGGAAGTATGGGAGGATCCGATGGCGTTTAAGCCAGAGAGATTCGTGAGCAGCGATGGAGAGGCGACGACGATGACGTTTGATGTGACATGTAGCAAGGAGATCAAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGGGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAGATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAACCCTCTCAAAGCCCATATACATCCAAGGTTTTAG

Coding sequence (CDS)

ATGTGCTTTGGGGACAAGCTGAATGAGACAGAGATCTTGGAAATTCAACAAGTGCAGCGTGAGATTTTGGTAAATCTTCAGCGTTTTGTTATACTCAATTTCTGGCCTAAGTTGATGAAGATTTTGCTTCGAAAGCGTTGGAAGGCTTACTTACAACTCAAAAGCAAGCAAGAAAAGGTCCTAATCCCATTTATCAAGGCACGAGAGAAGGCAAAACAAGAAACATCAAAAAAAGATGGAGAAGAAAAAGAAGAATTTGTAGTATCATATGTGGATACTCTGCTCGATTTGCACCTTATCGATGAAAATAGAAAGCTTAAAAATGAAGAAATGGTGAGTTTATGCTCGGAGTTCCTCAACGCCGGGACTGACACCACTGCCACAACTTTGCAATGGATAATGGCAAATTTAGTAAAGTACCCACACATCCAACAAAAACTATTCCAAGAGATCAAACGAGTCATGGAATCCAACACAAAACAACATGGGTTACGGGAGGAGGAGGTAAAGGAAGAAGATTTGGGGAAGCTTCCATATTTGAAAGCCGTGGTTCTAGAAGGATTACGAAGGCACCCGCCGGGACATTTCATGTTACCACATGCCGTGAAAGAAGATACGATGTTGGAGAATTATTTCATTCCCAAAAATGGAACGGTGAATATCTTGGCCGTGGAGATGGGGTGGGATCCGGAAGTATGGGAGGATCCGATGGCGTTTAAGCCAGAGAGATTCGTGAGCAGCGATGGAGAGGCGACGACGATGACGTTTGATGTGACATGTAGCAAGGAGATCAAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGGGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAGATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAACCCTCTCAAAGCCCATATACATCCAAGGTTTTAG

Protein sequence

MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKVLIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF
Homology
BLAST of Lcy02g000890 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-112
Identity = 200/330 (60.61%), Postives = 258/330 (78.18%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I E++ +QR  L++L +F I N WPK  K++LRKRW+ +LQ++ +Q  V
Sbjct: 186 MCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWPKFTKLILRKRWQEFLQIRRQQRDV 245

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+P I+AR K  +E  + + E+K+++V SYVDTLLDL L +ENRKL  E++++LCSEFL 
Sbjct: 246 LLPLIRARRKIVEERKRSEQEDKKDYVQSYVDTLLDLELPEENRKLNEEDIMNLCSEFLT 305

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTAT LQWIMANLVKYP IQ++L +EIK V+       G   +EV+EED+ K+PYL
Sbjct: 306 AGTDTTATALQWIMANLVKYPEIQERLHEEIKSVV-------GEEAKEVEEEDVEKMPYL 365

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAVVLEGLRRHPPGHF+LPH+V EDT+L  Y +PKNGT+N +  E+G DP  WE+PMAFK
Sbjct: 366 KAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFK 425

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+  + EA     D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK
Sbjct: 426 PERFMGEE-EAV----DLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWK 485

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
            V+G E+DL+EK+EFT+VMK+PLKA   PR
Sbjct: 486 EVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

BLAST of Lcy02g000890 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.2e-99
Identity = 181/337 (53.71%), Postives = 246/337 (73.00%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFG+KL + EI EI++ Q ++L++  +F +LN +P + K LLR++WK +L+L+  QE V
Sbjct: 191 MCFGEKLRKEEIREIEEAQYQMLISYTKFSVLNIFPSVTKFLLRRKWKEFLELRKSQESV 250

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDE------NRKLKNEEMVSL 120
           ++ ++ AR K           E    V+ YVDTLL+L +  E       RKL + E+VSL
Sbjct: 251 ILRYVNARSK-----------ETTGDVLCYVDTLLNLEIPTEEKEGGKKRKLSDSEIVSL 310

Query: 121 CSEFLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDL 180
           CSEFLNA TD TAT++QWIMA +VKYP IQ+K+++E+K V     ++     EE++EEDL
Sbjct: 311 CSEFLNAATDPTATSMQWIMAIMVKYPEIQRKVYEEMKTVFAGEEEE----REEIREEDL 370

Query: 181 GKLPYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWE 240
           GKL YLKAV+LE LRRHPPGH++  H V  DT+L  + IP+ GT+N +  EMG DP++WE
Sbjct: 371 GKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWE 430

Query: 241 DPMAFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLV 300
           DP+ FKPERF+  +GEA    FD+T ++EIKMMPFGAGRR+CPG  L++LHLEY+VANLV
Sbjct: 431 DPLTFKPERFL-ENGEA--CDFDMTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLV 490

Query: 301 WRFEWKAVEGDEIDLSEKMEF-TIVMKNPLKAHIHPR 331
           W+FEWK VEG+E+DLSEK +F T+VMKNP KA+I+PR
Sbjct: 491 WKFEWKCVEGEEVDLSEKQQFITMVMKNPFKANIYPR 509

BLAST of Lcy02g000890 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.9e-63
Identity = 137/332 (41.27%), Postives = 199/332 (59.94%), Query Frame = 0

Query: 1   MCFGDKLNETEILE-IQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEK 60
           MCFG +++  E++E + Q+ +++L+ L    I +F P L   +  K+ K   +++ +Q +
Sbjct: 186 MCFGVEMDNEEMIERVDQMMKDVLIVLDP-RIDDFLPILRLFVGYKQRKRVNEVRKRQIE 245

Query: 61  VLIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFL 120
            L+P I+ R    Q      G +K     SY+DTL D+ +        N E+V+LCSEFL
Sbjct: 246 TLVPLIEKRRSVVQ----NPGSDKTAASFSYLDTLFDVKVEGRKSGPTNAELVTLCSEFL 305

Query: 121 NAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPY 180
           N GTDTTAT L+W +  L++ P IQ +L+QEIK +         + +++V E D+ K+PY
Sbjct: 306 NGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTI---------VGDKKVDENDIEKMPY 365

Query: 181 LKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAF 240
           L AVV E LR+HPP +F L H+V E   L  Y IP +  V      +  DP VW DP  F
Sbjct: 366 LNAVVKELLRKHPPTYFTLTHSVTEPVKLAGYDIPMDTNVEFFVHGISHDPNVWSDPEKF 425

Query: 241 KPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 300
            P+RF+S   +A     D+T  KE+KMMPFG GRRICPG G+A +H+   +A +V  FEW
Sbjct: 426 DPDRFLSGREDA-----DITGVKEVKMMPFGVGRRICPGLGMATVHVNLMLARMVQEFEW 485

Query: 301 KAVEG-DEIDLSEKMEFTIVMKNPLKAHIHPR 331
            A  G +++D SEK+EFT+VMKNPL+A +  R
Sbjct: 486 FAYPGNNKVDFSEKLEFTVVMKNPLRAKVKLR 498

BLAST of Lcy02g000890 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.7e-62
Identity = 138/331 (41.69%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFG +++E  +  I QV + +L+ L    I ++ P L     ++R KA L+++ +Q + 
Sbjct: 196 MCFGLEMDEETVERIDQVMKSVLITLDP-RIDDYLPILSPFFSKQRKKA-LEVRREQVEF 255

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+P I+ R +A Q      G +      SY+DTL DL +  +     + E+VSLCSEFLN
Sbjct: 256 LVPIIEQRRRAIQ----NPGSDHTATTFSYLDTLFDLKVEGKKSAPSDAELVSLCSEFLN 315

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
            GTDTTAT ++W +A L+  P++Q KL++EIKR          + E++V E+D+ K+PYL
Sbjct: 316 GGTDTTATAVEWGIAQLIANPNVQTKLYEEIKRT---------VGEKKVDEKDVEKMPYL 375

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
            AVV E LR+HPP HF+L HAV E T L  Y IP +  V +    +  DP+ W +P  F 
Sbjct: 376 HAVVKELLRKHPPTHFVLTHAVTEPTTLGGYDIPIDANVEVYTPAIAEDPKNWLNPEKFD 435

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+S   EA     D+T    +KMMPFG GRRICPG  +A +H+   +A +V  FEW 
Sbjct: 436 PERFISGGEEA-----DITGVTGVKMMPFGVGRRICPGLAMATVHIHLMMARMVQEFEWG 495

Query: 301 AVEGD-EIDLSEKMEFTIVMKNPLKAHIHPR 331
           A   + ++D + K EFT+VMK  L+A I PR
Sbjct: 496 AYPPEKKMDFTGKWEFTVVMKESLRATIKPR 506

BLAST of Lcy02g000890 vs. ExPASy Swiss-Prot
Match: P37124 (Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.2e-60
Identity = 128/330 (38.79%), Postives = 190/330 (57.58%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFG +++E  I +I Q+ + +L+ L     L+ +  ++     K+ K  + ++ +Q K 
Sbjct: 201 MCFGVEMDEKTIEKIDQMMKSVLIALD--PRLDDYLPILSPFFSKQRKHAMDVRKQQIKT 260

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           ++PFI+ R+K  +        +K     SY+DTL DL +   N      E+V+LCSEFLN
Sbjct: 261 IVPFIEQRKKILESPE----IDKTAASFSYLDTLFDLKIEGRNSTPTYPELVTLCSEFLN 320

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
            GTDTTAT ++W +  L++ P+IQ +L++EIK+ +  N         ++ E+D+ K+PYL
Sbjct: 321 GGTDTTATAIEWAIGRLIENPNIQSQLYEEIKKTVGEN---------KIDEKDIEKMPYL 380

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
            AVV E LR+HPP +  L HAV E   L  Y IP    V I    +  DP +W +P  F 
Sbjct: 381 NAVVKELLRKHPPTYMSLTHAVTEPAKLGGYDIPTGVNVEIFLPGISDDPNLWSEPEKFD 440

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           P+RF     +A     D+T    +KM+PFG GRRICPG  +A +H+   +A LV  FEW 
Sbjct: 441 PDRFYLGKEDA-----DITGVSGVKMIPFGMGRRICPGLNMATVHVSLMLARLVQEFEWA 500

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
             E   +D +EK+EFT+VMKN L+A I PR
Sbjct: 501 DPENTRVDFTEKLEFTVVMKNTLRAKIKPR 510

BLAST of Lcy02g000890 vs. ExPASy TrEMBL
Match: A0A1S3ATZ4 (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.7e-156
Identity = 273/335 (81.49%), Postives = 308/335 (91.94%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNET+ILEIQ+VQR+ILVNLQRFV+LN WPKLMKILLR RWK YLQ+K++Q+KV
Sbjct: 192 MCFGDKLNETQILEIQEVQRDILVNLQRFVLLNLWPKLMKILLRNRWKEYLQIKNRQKKV 251

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+PFIKAREK K+E +K++GEEKEEFVVSYVDTLLDL LIDE RKLKNEE+VSLCSEFLN
Sbjct: 252 LVPFIKAREKVKEERAKREGEEKEEFVVSYVDTLLDLQLIDEKRKLKNEEIVSLCSEFLN 311

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQH-GLREEEVKEEDLGKLPY 180
           AGTDTTAT++QWIMANLVKYP IQ+KLFQEIK V ES+TK++ GLREEEVKEEDLGKLPY
Sbjct: 312 AGTDTTATSMQWIMANLVKYPKIQEKLFQEIKGVKESSTKKNGGLREEEVKEEDLGKLPY 371

Query: 181 LKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAF 240
           LKAV+LEGLRRHPPGHF+LPHAVKEDT+ ENYFIPKNG V+I AV MGWDP+VWEDPMAF
Sbjct: 372 LKAVILEGLRRHPPGHFILPHAVKEDTIFENYFIPKNGIVSIFAVGMGWDPQVWEDPMAF 431

Query: 241 KPERFVSSDG---EATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWR 300
           KPERF+S DG      + TFD+T +KEIKMMPFGAGRR+CP A LAMLHLEYFVANL+WR
Sbjct: 432 KPERFMSDDGGREAGYSTTFDITGNKEIKMMPFGAGRRMCPAASLAMLHLEYFVANLIWR 491

Query: 301 FEWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           FEWKAVEG+E+DLSEKMEFT+VMK PLKAHI+PRF
Sbjct: 492 FEWKAVEGEEVDLSEKMEFTVVMKKPLKAHINPRF 526

BLAST of Lcy02g000890 vs. ExPASy TrEMBL
Match: A0A6J1I6F9 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.6e-154
Identity = 271/331 (81.87%), Postives = 300/331 (90.63%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGD+LNET+ILEIQQVQREI+VNLQRF+ILN WPKLMKILLRKRWK YLQLK+KQ +V
Sbjct: 188 MCFGDELNETQILEIQQVQREIIVNLQRFLILNLWPKLMKILLRKRWKEYLQLKNKQHEV 247

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIP IKAREK K E + K+GEEKEE +VSYVDTL+DL LID+ RKL NEE+V+LCSEFLN
Sbjct: 248 LIPIIKAREKVKHEIT-KEGEEKEENLVSYVDTLIDLELIDQKRKLNNEEIVNLCSEFLN 307

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTATTLQWIMANLVK P IQQKLF+EI  +M+S T ++GLRE+EVKEEDLGKLPYL
Sbjct: 308 AGTDTTATTLQWIMANLVKNPQIQQKLFEEIDGIMKSCTTRNGLREDEVKEEDLGKLPYL 367

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHF+LPHAVKEDT LENYFIPKNGTVNI++ EMGWDPEVWEDPM FK
Sbjct: 368 KAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFK 427

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+S DGE ++  FD+T SKEIKMMPFG GRRICPG GLAMLHLEYFVANLVWRFEWK
Sbjct: 428 PERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWK 487

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           AVEGDE+DLSEKMEFTI MKNPLKA + PRF
Sbjct: 488 AVEGDEVDLSEKMEFTIWMKNPLKAQLRPRF 517

BLAST of Lcy02g000890 vs. ExPASy TrEMBL
Match: A0A6J1E305 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.9e-154
Identity = 271/331 (81.87%), Postives = 298/331 (90.03%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGD+LNET+ILEI QVQREI+VNLQRF+ILN WPKLMKILLRKRWK YLQLK+KQ +V
Sbjct: 188 MCFGDELNETQILEIHQVQREIVVNLQRFLILNLWPKLMKILLRKRWKEYLQLKNKQHEV 247

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIP IKAREK K E + K+GEEKEE +VSYVDTL+DL LID+ RKL NEE+V+LCSEFLN
Sbjct: 248 LIPVIKAREKVKHEIT-KEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLN 307

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTT TTLQWIMANLVK P IQQKLF+EI  +M+S+T +H LREEEVKEEDLGKLPYL
Sbjct: 308 AGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHELREEEVKEEDLGKLPYL 367

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHF+LPHAVKEDT LENYFIPKNGTVNI++ +MGWDPEVWEDPM FK
Sbjct: 368 KAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGDMGWDPEVWEDPMTFK 427

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERFVS DGEA +  FD+T SKEIKMMPFG GRRICPG GLAMLHLEYFVANLVWRFEWK
Sbjct: 428 PERFVSGDGEALSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWK 487

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           AVEGDE+DLSEKMEFTI MKNPLKA + PRF
Sbjct: 488 AVEGDEVDLSEKMEFTIWMKNPLKAQLRPRF 517

BLAST of Lcy02g000890 vs. ExPASy TrEMBL
Match: A0A6J1E669 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430324 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.2e-153
Identity = 269/330 (81.52%), Postives = 298/330 (90.30%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGD+LNET+IL+IQQVQREI+VNLQRF+ILNFWPKLMKILLRKRWK YLQLK+KQ +V
Sbjct: 188 MCFGDELNETQILDIQQVQREIIVNLQRFLILNFWPKLMKILLRKRWKEYLQLKNKQHEV 247

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIP IKAREK K E + K+GEEKEE +VSYVDTL+DL LID+ RKL NEE+V+LCSEFLN
Sbjct: 248 LIPIIKAREKVKHEIT-KEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLN 307

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTT TTLQWIMANLVK P IQQKLF+EI  +M+S+T QH L EEEVKEEDLGKLPYL
Sbjct: 308 AGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTQHELLEEEVKEEDLGKLPYL 367

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHF+LPHAVKEDT LENYFIPKNGTVNI++ EMGWDPEVWEDPM FK
Sbjct: 368 KAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFK 427

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+S +GE ++  FD+T SKEIKMMPFG GRRICPG GLAMLHLEYFVANLVWRFEWK
Sbjct: 428 PERFMSGEGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWK 487

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
           AVEGDE+DLSEKMEFTI MKNPLKA + PR
Sbjct: 488 AVEGDEVDLSEKMEFTIWMKNPLKAQLRPR 516

BLAST of Lcy02g000890 vs. ExPASy TrEMBL
Match: A0A6J1H044 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111459225 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.5e-152
Identity = 275/332 (82.83%), Postives = 296/332 (89.16%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNE EI+EIQQVQ+E+LVNLQRFV+LNFWPKLMKILLR RWKAYL LK+KQ+KV
Sbjct: 185 MCFGDKLNEKEIMEIQQVQQEMLVNLQRFVLLNFWPKLMKILLRSRWKAYLHLKAKQQKV 244

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+PFIKAREKAK E +K     +EE VVSYVDTLLDL L  ENRKLK+EEMV+LCSEFLN
Sbjct: 245 LMPFIKAREKAKHERAK-----EEEHVVSYVDTLLDLQLTHENRKLKDEEMVNLCSEFLN 304

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLR-EEEVKEEDLGKLPY 180
           AGTDTTATTLQWIMANLVKYP IQ KL++EIK VM+  ++QHG + EEEVKEEDL KLPY
Sbjct: 305 AGTDTTATTLQWIMANLVKYPEIQHKLYEEIKGVMK-ESQQHGSKVEEEVKEEDLEKLPY 364

Query: 181 LKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAF 240
           LKAVVLEGLRRHPPGHF+LPHAVKEDTMLENYFIPKNGTVNILA EMGWDPEVWEDPMAF
Sbjct: 365 LKAVVLEGLRRHPPGHFVLPHAVKEDTMLENYFIPKNGTVNILAAEMGWDPEVWEDPMAF 424

Query: 241 KPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 300
           KPERFV   GEA  M FD+T SKEIKMMPFG GRR+CP AGLAMLHLEYFVANLVWRFEW
Sbjct: 425 KPERFVGGGGEAEAMAFDITGSKEIKMMPFGVGRRMCPAAGLAMLHLEYFVANLVWRFEW 484

Query: 301 KAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           KAVEG E+DLSEKMEFTIVM NPLK HI PRF
Sbjct: 485 KAVEGVEVDLSEKMEFTIVMNNPLKPHIRPRF 510

BLAST of Lcy02g000890 vs. NCBI nr
Match: XP_038877784.1 (cytochrome P450 89A2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 582.4 bits (1500), Expect = 2.5e-162
Identity = 294/332 (88.55%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNET+ILEIQQVQREILVNLQRFVILN WPKLMKILLRKRWK YLQLKSKQ+KV
Sbjct: 190 MCFGDKLNETQILEIQQVQREILVNLQRFVILNLWPKLMKILLRKRWKEYLQLKSKQQKV 249

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIPFIKAREK K+E SK   EEKEEFVVSYVDTLLDL LIDE RKLKNEEMV+LCSEFLN
Sbjct: 250 LIPFIKAREKVKEERSKTK-EEKEEFVVSYVDTLLDLQLIDEKRKLKNEEMVNLCSEFLN 309

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTATTLQWIMANLVKYP IQQKLF+EIK V  S+TKQH LREEEVKEEDLGKLPYL
Sbjct: 310 AGTDTTATTLQWIMANLVKYPQIQQKLFEEIKGVKGSSTKQHELREEEVKEEDLGKLPYL 369

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHFMLPHAVKEDT LENYFIPKNG VNI AVEMGWDP+VWEDPMAFK
Sbjct: 370 KAVILEGLRRHPPGHFMLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFK 429

Query: 241 PERFVSS-DGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 300
           PERF+S+  GE T+ TFD+T SKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW
Sbjct: 430 PERFMSNVGGETTSTTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 489

Query: 301 KAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           KA+EG E+DLSEKMEFTIVMKNPLKA+IHPRF
Sbjct: 490 KAMEG-EVDLSEKMEFTIVMKNPLKAYIHPRF 519

BLAST of Lcy02g000890 vs. NCBI nr
Match: XP_038877782.1 (cytochrome P450 89A2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 582.4 bits (1500), Expect = 2.5e-162
Identity = 294/332 (88.55%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNET+ILEIQQVQREILVNLQRFVILN WPKLMKILLRKRWK YLQLKSKQ+KV
Sbjct: 193 MCFGDKLNETQILEIQQVQREILVNLQRFVILNLWPKLMKILLRKRWKEYLQLKSKQQKV 252

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIPFIKAREK K+E SK   EEKEEFVVSYVDTLLDL LIDE RKLKNEEMV+LCSEFLN
Sbjct: 253 LIPFIKAREKVKEERSKTK-EEKEEFVVSYVDTLLDLQLIDEKRKLKNEEMVNLCSEFLN 312

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTATTLQWIMANLVKYP IQQKLF+EIK V  S+TKQH LREEEVKEEDLGKLPYL
Sbjct: 313 AGTDTTATTLQWIMANLVKYPQIQQKLFEEIKGVKGSSTKQHELREEEVKEEDLGKLPYL 372

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHFMLPHAVKEDT LENYFIPKNG VNI AVEMGWDP+VWEDPMAFK
Sbjct: 373 KAVILEGLRRHPPGHFMLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFK 432

Query: 241 PERFVSS-DGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 300
           PERF+S+  GE T+ TFD+T SKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW
Sbjct: 433 PERFMSNVGGETTSTTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 492

Query: 301 KAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           KA+EG E+DLSEKMEFTIVMKNPLKA+IHPRF
Sbjct: 493 KAMEG-EVDLSEKMEFTIVMKNPLKAYIHPRF 522

BLAST of Lcy02g000890 vs. NCBI nr
Match: XP_008437556.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis melo])

HSP 1 Score: 562.0 bits (1447), Expect = 3.4e-156
Identity = 273/335 (81.49%), Postives = 308/335 (91.94%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNET+ILEIQ+VQR+ILVNLQRFV+LN WPKLMKILLR RWK YLQ+K++Q+KV
Sbjct: 192 MCFGDKLNETQILEIQEVQRDILVNLQRFVLLNLWPKLMKILLRNRWKEYLQIKNRQKKV 251

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+PFIKAREK K+E +K++GEEKEEFVVSYVDTLLDL LIDE RKLKNEE+VSLCSEFLN
Sbjct: 252 LVPFIKAREKVKEERAKREGEEKEEFVVSYVDTLLDLQLIDEKRKLKNEEIVSLCSEFLN 311

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQH-GLREEEVKEEDLGKLPY 180
           AGTDTTAT++QWIMANLVKYP IQ+KLFQEIK V ES+TK++ GLREEEVKEEDLGKLPY
Sbjct: 312 AGTDTTATSMQWIMANLVKYPKIQEKLFQEIKGVKESSTKKNGGLREEEVKEEDLGKLPY 371

Query: 181 LKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAF 240
           LKAV+LEGLRRHPPGHF+LPHAVKEDT+ ENYFIPKNG V+I AV MGWDP+VWEDPMAF
Sbjct: 372 LKAVILEGLRRHPPGHFILPHAVKEDTIFENYFIPKNGIVSIFAVGMGWDPQVWEDPMAF 431

Query: 241 KPERFVSSDG---EATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWR 300
           KPERF+S DG      + TFD+T +KEIKMMPFGAGRR+CP A LAMLHLEYFVANL+WR
Sbjct: 432 KPERFMSDDGGREAGYSTTFDITGNKEIKMMPFGAGRRMCPAASLAMLHLEYFVANLIWR 491

Query: 301 FEWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           FEWKAVEG+E+DLSEKMEFT+VMK PLKAHI+PRF
Sbjct: 492 FEWKAVEGEEVDLSEKMEFTVVMKKPLKAHINPRF 526

BLAST of Lcy02g000890 vs. NCBI nr
Match: XP_038877785.1 (cytochrome P450 89A2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-155
Identity = 284/332 (85.54%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKLNET+ILEIQQVQREILVNLQRFVILN WPKLMKILLRKRWK YLQLKSKQ+KV
Sbjct: 190 MCFGDKLNETQILEIQQVQREILVNLQRFVILNLWPKLMKILLRKRWKEYLQLKSKQQKV 249

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIPFIKAREK K+E SK   EEKEEFVVSYVDTLLDL LIDE RKLKNEEMV+LCSEFLN
Sbjct: 250 LIPFIKAREKVKEERSKTK-EEKEEFVVSYVDTLLDLQLIDEKRKLKNEEMVNLCSEFLN 309

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTATTLQWIMANLVKYP IQQKLF+EIK          G++  +VKEEDLGKLPYL
Sbjct: 310 AGTDTTATTLQWIMANLVKYPQIQQKLFEEIK----------GVKGSKVKEEDLGKLPYL 369

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHFMLPHAVKEDT LENYFIPKNG VNI AVEMGWDP+VWEDPMAFK
Sbjct: 370 KAVILEGLRRHPPGHFMLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFK 429

Query: 241 PERFVSS-DGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 300
           PERF+S+  GE T+ TFD+T SKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW
Sbjct: 430 PERFMSNVGGETTSTTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEW 489

Query: 301 KAVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           KA+EG E+DLSEKMEFTIVMKNPLKA+IHPRF
Sbjct: 490 KAMEG-EVDLSEKMEFTIVMKNPLKAYIHPRF 509

BLAST of Lcy02g000890 vs. NCBI nr
Match: KAG6579336.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.1 bits (1437), Expect = 4.9e-155
Identity = 273/331 (82.48%), Postives = 300/331 (90.63%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGD+LNET+ILEIQQVQREI+VNLQRF+ILNFWPKLMKILLRKRWK YLQLK+KQ +V
Sbjct: 188 MCFGDELNETQILEIQQVQREIIVNLQRFLILNFWPKLMKILLRKRWKEYLQLKNKQHEV 247

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           LIP IKAREK K E + K+GEEKEE +VSYVDTL+DL LID+ RKL NEE+V+LCSEFLN
Sbjct: 248 LIPIIKAREKVKHEIT-KEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLN 307

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTT TTLQWIMANLVK P IQQKLF+EI  +M+S+T QH LREEEVKEEDLGKLPYL
Sbjct: 308 AGTDTTTTTLQWIMANLVKNPPIQQKLFEEIDVIMKSSTTQHELREEEVKEEDLGKLPYL 367

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAV+LEGLRRHPPGHF+LPHAVKEDT LENYFIPKNGTVNI++ EMGWDPEVWEDPM FK
Sbjct: 368 KAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFK 427

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+S DGE ++  FD+T SKEIKMMPFG GRRICPG GLAMLHLEYFVANLVWRFEWK
Sbjct: 428 PERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWK 487

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPRF 332
           AVEGDE+DLSEKMEFTI MKNPLKA + PRF
Sbjct: 488 AVEGDEVDLSEKMEFTIWMKNPLKAQLRPRF 517

BLAST of Lcy02g000890 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 407.9 bits (1047), Expect = 7.7e-114
Identity = 200/330 (60.61%), Postives = 258/330 (78.18%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I E++ +QR  L++L +F I N WPK  K++LRKRW+ +LQ++ +Q  V
Sbjct: 186 MCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWPKFTKLILRKRWQEFLQIRRQQRDV 245

Query: 61  LIPFIKAREKAKQETSKKDGEEKEEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSEFLN 120
           L+P I+AR K  +E  + + E+K+++V SYVDTLLDL L +ENRKL  E++++LCSEFL 
Sbjct: 246 LLPLIRARRKIVEERKRSEQEDKKDYVQSYVDTLLDLELPEENRKLNEEDIMNLCSEFLT 305

Query: 121 AGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKLPYL 180
           AGTDTTAT LQWIMANLVKYP IQ++L +EIK V+       G   +EV+EED+ K+PYL
Sbjct: 306 AGTDTTATALQWIMANLVKYPEIQERLHEEIKSVV-------GEEAKEVEEEDVEKMPYL 365

Query: 181 KAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPMAFK 240
           KAVVLEGLRRHPPGHF+LPH+V EDT+L  Y +PKNGT+N +  E+G DP  WE+PMAFK
Sbjct: 366 KAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFK 425

Query: 241 PERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWK 300
           PERF+  + EA     D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK
Sbjct: 426 PERFMGEE-EAV----DLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWK 485

Query: 301 AVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
            V+G E+DL+EK+EFT+VMK+PLKA   PR
Sbjct: 486 EVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

BLAST of Lcy02g000890 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 403.3 bits (1035), Expect = 1.9e-112
Identity = 199/333 (59.76%), Postives = 251/333 (75.38%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I +++ VQR  L+   RF ILN WPK  K++LRKRW+ + Q++ +Q  V
Sbjct: 189 MCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMRREQHDV 248

Query: 61  LIPFIKAREKAKQETSKKDGEEKE---EFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSE 120
           L+P I+AR K  +E   +  EE+E   E+V SYVDTLL+L L DE RKL  +E+VSLCSE
Sbjct: 249 LLPLIRARRKIVEERKNRSSEEEEDNKEYVQSYVDTLLELELPDEKRKLNEDEIVSLCSE 308

Query: 121 FLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKL 180
           FLN GTDTTAT LQWIMANLVK P IQ++L++EIK V+       G    EV+EED  K+
Sbjct: 309 FLNGGTDTTATALQWIMANLVKNPDIQKRLYEEIKSVV-------GEEANEVEEEDAQKM 368

Query: 181 PYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPM 240
           PYL+AVV+EGLRRHPPGHF+LPH+V EDT+L  Y +PKNGT+N +  E+G DP+VWE+PM
Sbjct: 369 PYLEAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPKVWEEPM 428

Query: 241 AFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRF 300
           AFKPERF+           D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F
Sbjct: 429 AFKPERFMEE-------AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 488

Query: 301 EWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
           +WK V+G E+DL+EK+EFT+VMK+PLKA   PR
Sbjct: 489 DWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 507

BLAST of Lcy02g000890 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 401.0 bits (1029), Expect = 9.4e-112
Identity = 197/333 (59.16%), Postives = 252/333 (75.68%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I +++ VQR  L+   RF IL  WPK  K++ RKRW+ + Q++S+Q+ V
Sbjct: 190 MCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILTLWPKFTKLIYRKRWEEFFQMQSEQQDV 249

Query: 61  LIPFIKAREKAKQETSKKDGEEK---EEFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSE 120
           L+P I+AR K   E  K+  EE+   +E+V SYVDTLLD+ L DE RKL  +E+VSLCSE
Sbjct: 250 LLPLIRARRKIVDERKKRSSEEEKDNKEYVQSYVDTLLDVELPDEKRKLNEDEIVSLCSE 309

Query: 121 FLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKL 180
           FLNAGTDTTAT LQWIMANLVK P IQ++L++EIK ++       G   +EV+E+D  K+
Sbjct: 310 FLNAGTDTTATALQWIMANLVKNPEIQRRLYEEIKSIV-------GEEAKEVEEQDAQKM 369

Query: 181 PYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPM 240
           PYLKAVV+EGLRRHPPGHF+LPH+V EDT+L  Y +PK GT+N +  E+G DP+VWE+PM
Sbjct: 370 PYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRDPKVWEEPM 429

Query: 241 AFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRF 300
           AFKPERF+           D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F
Sbjct: 430 AFKPERFMEE-------AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 489

Query: 301 EWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
           EW+ V+G E+DL+EK+EFT+VMK+PLKA   PR
Sbjct: 490 EWQEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 508

BLAST of Lcy02g000890 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-110
Identity = 198/328 (60.37%), Postives = 249/328 (75.91%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I +++ VQR  L+   RF ILN WPK  K++LRKRW+ + Q++ +Q  V
Sbjct: 189 MCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMRREQHDV 248

Query: 61  LIPFIKAREKAKQETSKKDGEEKEE---FVVSYVDTLLDLHLIDENRKLKNEEMVSLCSE 120
           L+P I+AR K  +E   +  EE+E+   +V SYVDTLL+L L DE RKL  +E+VSLCSE
Sbjct: 249 LLPLIRARRKIVEERKNRSSEEEEDNKVYVQSYVDTLLELELPDEKRKLNEDEIVSLCSE 308

Query: 121 FLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKL 180
           FLN GTDTTAT LQWIMANLVK P IQ++L++EIK V+       G   +EV+EED  K+
Sbjct: 309 FLNGGTDTTATALQWIMANLVKNPEIQKRLYEEIKSVV-------GEEAKEVEEEDAQKM 368

Query: 181 PYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPM 240
           PYLKAVV+EGLRRHPPGHF+LPH+V EDT+L  Y +PK GT+N +  E+G DP VWE+PM
Sbjct: 369 PYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRDPMVWEEPM 428

Query: 241 AFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRF 300
           AFKPERF+  + EA     D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F
Sbjct: 429 AFKPERFMGEE-EAV----DITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 488

Query: 301 EWKAVEGDEIDLSEKMEFTIVMKNPLKA 326
           EWK V+G E+DL+EK EFT+VMK+ LKA
Sbjct: 489 EWKEVQGHEVDLTEKFEFTVVMKHSLKA 504

BLAST of Lcy02g000890 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 379.0 bits (972), Expect = 3.8e-105
Identity = 192/333 (57.66%), Postives = 250/333 (75.08%), Query Frame = 0

Query: 1   MCFGDKLNETEILEIQQVQREILVNLQRFVILNFWPKLMKILLRKRWKAYLQLKSKQEKV 60
           MCFGDKL+E +I +++ VQR++L+   R+ ILN  PK  K++LRKRW+ + Q++ +Q+ V
Sbjct: 188 MCFGDKLDEKQIKQVEYVQRQMLLGFARYSILNLCPKFTKLILRKRWEEFFQMRREQQDV 247

Query: 61  LIPFIKAREKAKQETSKKDGEEKE---EFVVSYVDTLLDLHLIDENRKLKNEEMVSLCSE 120
           L+  I AR K  +E  K+  EE+E   E+V SYVDTLLD+ L DE RKL  +E+VSLCSE
Sbjct: 248 LLRLIYARRKIVEERKKRSSEEEEENKEYVQSYVDTLLDVELPDEKRKLNEDEIVSLCSE 307

Query: 121 FLNAGTDTTATTLQWIMANLVKYPHIQQKLFQEIKRVMESNTKQHGLREEEVKEEDLGKL 180
           FL AG+DTTAT LQWIMANLVK   IQ++L++EI  V+       G   + V+E+D  K+
Sbjct: 308 FLIAGSDTTATVLQWIMANLVKNQEIQERLYEEITNVV-------GEEAKVVEEKDTQKM 367

Query: 181 PYLKAVVLEGLRRHPPGHFMLPHAVKEDTMLENYFIPKNGTVNILAVEMGWDPEVWEDPM 240
           PYLKAVV+E LRRHPPG+ +LPH+V EDT+L  Y +PK GT+N L  E+G DP+VWE+PM
Sbjct: 368 PYLKAVVMEALRRHPPGNTVLPHSVTEDTVLGGYKVPKKGTINFLVAEIGRDPKVWEEPM 427

Query: 241 AFKPERFVSSDGEATTMTFDVTCSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRF 300
           AFKPERF+  + EA     D+T S+ IKMMPFGAGRRICPG GLAMLHLEY+VAN+V  F
Sbjct: 428 AFKPERFMGEE-EAV----DITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 487

Query: 301 EWKAVEGDEIDLSEKMEFTIVMKNPLKAHIHPR 331
           +WK VEG E+DL+EK+EFT++MK+PLKA   PR
Sbjct: 488 QWKEVEGHEVDLTEKVEFTVIMKHPLKAIAVPR 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426021.1e-11260.61Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
Q9SRQ15.2e-9953.71Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
P371237.9e-6341.27Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
O489286.7e-6241.69Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
P371246.2e-6038.79Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3ATZ41.7e-15681.49cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1[more]
A0A6J1I6F92.6e-15481.87cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1[more]
A0A6J1E3055.9e-15481.87cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=... [more]
A0A6J1E6692.2e-15381.52cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430324 PE=3 SV=... [more]
A0A6J1H0442.5e-15282.83cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111459225 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_038877784.12.5e-16288.55cytochrome P450 89A2-like isoform X2 [Benincasa hispida][more]
XP_038877782.12.5e-16288.55cytochrome P450 89A2-like isoform X1 [Benincasa hispida][more]
XP_008437556.13.4e-15681.49PREDICTED: cytochrome P450 89A2-like [Cucumis melo][more]
XP_038877785.12.2e-15585.54cytochrome P450 89A2-like isoform X3 [Benincasa hispida][more]
KAG6579336.14.9e-15582.48Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G64900.17.7e-11460.61cytochrome P450, family 89, subfamily A, polypeptide 2 [more]
AT1G64950.11.9e-11259.76cytochrome P450, family 89, subfamily A, polypeptide 5 [more]
AT1G64940.19.4e-11259.16cytochrome P450, family 87, subfamily A, polypeptide 6 [more]
AT2G12190.11.0e-11060.37Cytochrome P450 superfamily protein [more]
AT1G64930.13.8e-10557.66cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 276..299
score: 33.08
coord: 266..276
score: 58.26
coord: 130..156
score: 33.72
coord: 181..199
score: 31.3
coord: 110..127
score: 26.77
coord: 222..246
score: 32.76
IPR001128Cytochrome P450PRINTSPR00385P450coord: 121..138
score: 43.0
coord: 276..287
score: 38.07
coord: 267..276
score: 60.88
coord: 182..193
score: 40.67
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..322
e-value: 6.9E-70
score: 236.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..331
e-value: 2.6E-80
score: 272.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 1..330
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 1..328
NoneNo IPR availablePANTHERPTHR24298:SF850CYTOCHROME P450coord: 1..328
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 269..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g000890.1Lcy02g000890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen