Lcy02g000210 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g000210
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Cytochrome p450
Location	Chr02: 505391 .. 508193 (+)
RNA-Seq Expression	Lcy02g000210
Synteny	Lcy02g000210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTTTTAGTGCACAAAAAGCTTAAGAGAAATGGGTTTGGAGGTCCAACGCCAAGTTTTCCTCTTGGAAATATTAGTGAGATGAAAAGGAACAACCTTATTACTCCTATTTTTGAGTCTTCTCAACTTACCCACAACATACATTCTATCGTCTTTCCCTATTTTTCTCGCTGGCAAACTGCCCATGGTAAGCCATCGCCCTACCATTATATATATAGAGTTACATTATTGTGTAGTTTGAGATTAGATGTTGTCTTTGTTTATAAACTCATAATGCTGAACTCTAATAATTGCATTTCCATGCATTTTTTTTTCTCAATATATCCAAACTTAGTAATTATTTTTGAATGTGATACAGGGAAAATATTTACATATTGGTTGGGGACAGAGCCATTCTTGTACATTGCAGACCCAGAATTTCTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTGATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGTTAGTTTCTAATTCTCTTTTTTCATCCTCTTTTAAACCTTTCATATTAAACCATCCTAATCAAGTTTCTTAAGTTGTTTTTTATTCCCATTTTGAGTTTTTTTCAAAACATAAATAAATGGACTACTTTATCAATTGAATTATTTTTAAATTGACTAACAAAAAAGAAAAATATTTGGCTGCTTACCGGTACGTAAGGATCTCCCTACATACCCATTAAAATGTTGACAATTAAAAAAGAGAAAGGTAGAAAATGAAAAAAAAAAAAATATATCTCTCCTAATTGTCAAGATTTTAATAGTAAGGGGATCTCGACAAAAAATTTCATACTACCTTCATCAATTATTTGTCATGAAAGAAGTACTTAAGAATGATATGTGACCCATGAAATAAAATATTATATCATAAATGTTCATTAAATGACATGTCAAATGTTGAATGGAAAGAAAATACGTACTATATTAGACATTTTTCGGAAGATTTCTACCATGGGCATGGTATAGTCAAGTATTGTCATAGATTTAAAACATGTCCTATTTTCAAACTATTGTCAATCACACATTCCCATGTGCTTTTCACTTCCCAAGTTGACATTCCTTGACCATTTCAAACATCTTCACTCACATCCACAAAATCTTCATTCCTTTTTGAAAACATCATAATTACTAATAACCCAATACATGTGTAAACAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCGAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGCGGCGGCGGTGCTGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAAGCTGAGTACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGCCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAAAAAGGTAAATACTATACACTTTATGCTTTGATTCTTTGTTTTTCTTTTCCTTGTATTAATTATGAATACTTAACACATATCCGTGAGTCGTTATCTCTATACACATCATCATTAAAATATTGACAACCATGAGAAAAAAAATCTCATTCTCAATTTTTTTTGTCTTAGTTGTCAACATTTCAATGATGGTATGTATGAAGATATCCACGTACTGTTATGCAGTCAAATATTGAATATGACAGAGAATTTAAAAAAATGCAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGACTGACAATCCCCAATGGCACCAACATGTGGATCGACGTGGTGGCCATGCATCACGACCAAGCCCTTTGGGGCGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACCTGACGTTTATGGAGTATAAGATTGTGTTGACGTTGATTTTGTCTAGGTTTTCCTTCTCCTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCGCTCCGACCTGCCCATGGGTTGCCCCTCATTTTTCAACTACTCCAACATTAGACTAGGATAAATTATTGGAGTAGATTGTCACAATGTGGTTCATAGTTTTATACGGTTGGATATATGAGAGGGAAATTATAAAACATATATATTTCAAACCTACACTCATAAGTCAACAGAGGCATACGGATTTCGAATCTTGCAAACAGTCGTCGTTATTAAGAGGTAGCAACTTTGTGGCGGTGTTGATCAATGATCATTTTTCTTA

mRNA sequence

CATTTTTAGTGCACAAAAAGCTTAAGAGAAATGGGTTTGGAGGTCCAACGCCAAGTTTTCCTCTTGGAAATATTAGTGAGATGAAAAGGAACAACCTTATTACTCCTATTTTTGAGTCTTCTCAACTTACCCACAACATACATTCTATCGTCTTTCCCTATTTTTCTCGCTGGCAAACTGCCCATGGGAAAATATTTACATATTGGTTGGGGACAGAGCCATTCTTGTACATTGCAGACCCAGAATTTCTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTGATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCGAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGCGGCGGCGGTGCTGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAAGCTGAGTACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGCCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAAAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGACTGACAATCCCCAATGGCACCAACATGTGGATCGACGTGGTGGCCATGCATCACGACCAAGCCCTTTGGGGCGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACCTGACGTTTATGGAGTATAAGATTGTGTTGACGTTGATTTTGTCTAGGTTTTCCTTCTCCTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCGCTCCGACCTGCCCATGGGTTGCCCCTCATTTTTCAACTACTCCAACATTAGACTAGGATAAATTATTGGAGTAGATTGTCACAATGTGGTTCATAGTTTTATACGGTTGGATATATGAGAGGGAAATTATAAAACATATATATTTCAAACCTACACTCATAAGTCAACAGAGGCATACGGATTTCGAATCTTGCAAACAGTCGTCGTTATTAAGAGGTAGCAACTTTGTGGCGGTGTTGATCAATGATCATTTTTCTTA

Coding sequence (CDS)

ATGAAAAGGAACAACCTTATTACTCCTATTTTTGAGTCTTCTCAACTTACCCACAACATACATTCTATCGTCTTTCCCTATTTTTCTCGCTGGCAAACTGCCCATGGGAAAATATTTACATATTGGTTGGGGACAGAGCCATTCTTGTACATTGCAGACCCAGAATTTCTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTGATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCGAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGCGGCGGCGGTGCTGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAAGCTGAGTACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGCCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAAAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGACTGACAATCCCCAATGGCACCAACATGTGGATCGACGTGGTGGCCATGCATCACGACCAAGCCCTTTGGGGCGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACCTGACGTTTATGGAGTATAAGATTGTGTTGACGTTGATTTTGTCTAGGTTTTCCTTCTCCTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCGCTCCGACCTGCCCATGGGTTGCCCCTCATTTTTCAACTACTCCAACATTAG

Protein sequence

MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLLLKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH

Homology

[Close]

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 163/452 (36.06%), Postives = 252/452 (55.75%), Query Frame = 0


Query: 13  SSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKP 72
           +   + N  + +FPYF  W   +G I+ Y  G+   L + DP  +K L+   K+   GKP
Sbjct: 79  TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELA-NCKSLDLGKP 138

Query: 73  SVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANL 132
              +++R ++ G G++ + GD WV QR VI P      +K M +LM+E+   ML  W N 
Sbjct: 139 CYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNE 198

Query: 133 V--LSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVP 192
           V    G  +I V++ + + + ++I++  FG     G+++F K+R LQ  + K + L+GVP
Sbjct: 199 VEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGVP 258

Query: 193 FAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLLLKESGEEGKL 252
            +  L     R    L   I  L    I+ + E+ ++ +V    DLL  +++ S ++G  
Sbjct: 259 GSRYLPTRSNRGIWNLDSSIRTLI-LNISKKYEHDSSTSV--NKDLLHSIIQGS-KDGPF 318

Query: 253 GRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDF 312
                   ++D CK  +F GHETT+    W L+LLA H EWQ++ R E   +   +  DF
Sbjct: 319 ASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLDF 378

Query: 313 DFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQ 372
           D   L +LKK+  V+ E LRLYP A  V R+A  D+ + G+ IP GTN+WI +   H D 
Sbjct: 379 DI--LRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDP 438

Query: 373 ALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSR 432
           ++WG   ++F P+RF N  IAG C     Y+PFG G R C G++L  +E K+VL+L+LS+
Sbjct: 439 SVWGPSADKFDPDRFAN-GIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSK 498

Query: 433 FSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
           F F LSP+Y H P+  L++ P  G+PLIF+ L
Sbjct: 499 FEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

[Close]

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 7.1e-78
Identity = 161/456 (35.31%), Postives = 257/456 (56.36%), Query Frame = 0


Query: 12  ESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTE---VKAKF 71
           +   + H+I   + P++  W   +GK F  W GT+P L + + E +K L  +   V  + 
Sbjct: 70  DCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRS 129

Query: 72  WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLER 131
           W    + ++  K+  G GL+MA G +W  QRH+  PAF    LK  +  MVE T+ ++ER
Sbjct: 130 W----LQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 189

Query: 132 WANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVG 191
               V  G  ++E+ +E+     +II++T FG   + G+++F  L  LQ    +  R + 
Sbjct: 190 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 249

Query: 192 VPFAGVLNAGKAREARRLGEEIDGLFRAVITARRE-NQAAAAVLQQHDLLSLLLKESGEE 251
            P +  L +   RE + L +E++ L   +I +RR+  +   +     DLL LLL E   +
Sbjct: 250 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 309

Query: 252 GKLGRKLSTTELI-DECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDK 311
                  +  +LI DECKTFFF GHETTAL LTWT +LLA +  WQ ++REE+++V G  
Sbjct: 310 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 369

Query: 312 HTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAM 371
                  +LS+L  +  V++E LRLYP A  + R A  D+ +  LTIP G ++WI V+A+
Sbjct: 370 GLP-SVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAI 429

Query: 372 HHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTL 431
           HH + LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI+L  
Sbjct: 430 HHSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILAT 489

Query: 432 ILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
           ++S+F+F++S +Y H+P ++L+++P +G+ +I + L
Sbjct: 490 LISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.2e-77
Identity = 155/455 (34.07%), Postives = 251/455 (55.16%), Query Frame = 0


Query: 24  VFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKPSVFKRDRKSMF 83
           +FPYF +W+ A+G+ + YWL   P LY+ DPE +  +   V     GKP   ++ ++ +F
Sbjct: 107 IFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDM-GKPKYLQKGQEPLF 166

Query: 84  GNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLV----LSGHPQ 143
           G G++ A G  W RQR VI P F  + ++AM  LMV++   ++  W + +     +   +
Sbjct: 167 GGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAE 226

Query: 144 IEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVLNAGK 203
           + V+ ++ S + ++I++  FG D+  GR++F +LR+L   + +T+ +  +P    L  GK
Sbjct: 227 VVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSLRHLPTGK 286

Query: 204 AREARRLGEEIDGLFRAVITARRENQAAAAVLQQH------------DLLSLLLKESGEE 263
            R   RL  EI  L   ++   RE + AA   ++H            D L  +++ SG  
Sbjct: 287 NRRIWRLTGEIRSLIMELV---RERRCAARAAREHGGKAAPPSPPERDFLGSIIENSG-- 346

Query: 264 GKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKH 323
              G+      ++D CK  +F GHET+A+  TW L+LLA H EWQ + R E+ +V G   
Sbjct: 347 ---GQPRPDDFVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDG 406

Query: 324 TDF----DFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDV 383
                  DF  +S+++ +G V+ E LRL+P +  V R+  RD+ +  L  P GT +++ V
Sbjct: 407 AAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPV 466

Query: 384 VAMHHDQALWGDQVNEFHPERFQNDAIAGGCSH-KMGYLPFGFGGRMCVGRHLTFMEYKI 443
             MHHD A WG     F P RF+ D +A  C H +  ++PFG G R C+G++L  +E K 
Sbjct: 467 STMHHDVAAWGPTARLFDPSRFR-DGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKT 526

Query: 444 VLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPL 458
           ++ ++L+RF F+LSP+Y HSP+  L + P  GL L
Sbjct: 527 LVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRL 551

[Close]

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.5e-77
Identity = 163/452 (36.06%), Postives = 251/452 (55.53%), Query Frame = 0


Query: 12  ESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGK 71
           + S + HNI   + P++  W   +GK F  W GTEP L + + E +K L T+      GK
Sbjct: 71  DCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK-HNPVTGK 130

Query: 72  PSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWAN 131
             + ++  K   G GL+MA G+ W  QRH+  PAF    LK  +  MVE T  M ER   
Sbjct: 131 SWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRK 190

Query: 132 LVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPF 191
            V     ++E+ +E+     +II++T FG     G+++F  L  LQ    +  R +  P 
Sbjct: 191 EV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG 250

Query: 192 AGVLNAGKAREARRLGEEIDGLFRAVITARREN-QAAAAVLQQHDLLSLLLKESGEEGKL 251
           +  L +   RE + L  E++ L   +I +R+++ +   +     DLL LLL +       
Sbjct: 251 SRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN--- 310

Query: 252 GRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDF 311
              L+   ++DECKTFFF GHETT+L LTWTL+LLA +  WQ  +R+E++QV G      
Sbjct: 311 KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVP- 370

Query: 312 DFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQ 371
              +LS L  +  V++E LRLYP A  + R A  DI +  L IP G ++WI V+A+HH  
Sbjct: 371 SVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSN 430

Query: 372 ALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSR 431
            LWG+  NEF+PERF   + A        ++PF  G R C+G+    ME KI+L +++S+
Sbjct: 431 ELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSK 490

Query: 432 FSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
           FSF++S +Y H+P ++L+++P +G+ L+ + L
Sbjct: 491 FSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 8.7e-76
Identity = 168/469 (35.82%), Postives = 264/469 (56.29%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKIL 60
           MKR + ++ +  SS   ++I   + P++ +W + +G+ F YW GTEP + I+DPE  K +
Sbjct: 61  MKRESHLSILDPSS---NDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTM 120

Query: 61  STEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVE 120
            +  K  F+ K        K +   GLV  EG +WVR R ++ PAF+   LK M+++MV+
Sbjct: 121 LSN-KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVD 180

Query: 121 STTHMLERW---ANLVLSGHPQI--EVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRD 180
            T  MLE W   +    + HP+I  E+ +E      +IIA ++FG  +  G +VF+   +
Sbjct: 181 CTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQME 240

Query: 181 LQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHD 240
           L+     +   V +P    L         +L  ++D   + +I++R ++++        D
Sbjct: 241 LKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKSDYG----DD 300

Query: 241 LLSLLLKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQL 300
           LL +LLK    EGK  RK+S  E+I EC+TFFFGGHETT+  L WT +LL++H +WQ +L
Sbjct: 301 LLGILLKAYNTEGK-ERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKL 360

Query: 301 REEIKQVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPN 360
           REEI +  G + T  D    S+LK M  V+ E LRLY     + R+A  +I +  L IP 
Sbjct: 361 REEIFKECGKEKTP-DSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 420

Query: 361 GTNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHL 420
           GT + I ++ MH D+ LWG   ++F+P RF N  ++   +H    L F  G R C+G++ 
Sbjct: 421 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFAN-GVSRAANHPNALLAFSVGPRACIGQNF 480

Query: 421 TFMEYKIVLTLILSRFSF-SLSPDYCHSPSIMLSLRPAHGLPLIFQLLQ 464
             +E K VLT+IL RF F SL  +Y H+P   ++++P +GLP++ Q L+
Sbjct: 481 VMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLE 518

[Close]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL
Match: A0A6J1H0W5 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 8.9e-241
Identity = 411/464 (88.58%), Postives = 440/464 (94.83%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKIL 60
           MKR +    +FES  LTH+IHSIVFPYFSRWQ AHGK+FTYWLGTEPFLY+A+PEF+K+L
Sbjct: 57  MKRTS-FDRVFES--LTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKML 116

Query: 61  STEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVE 120
           S EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVE
Sbjct: 117 SREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVE 176

Query: 121 STTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTL 180
           STT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTL
Sbjct: 177 STTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTL 236

Query: 181 FKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLL 240
           FKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQAAA V+QQ+DLLSLL
Sbjct: 237 FKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLL 296

Query: 241 LKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIK 300
           LKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLL IHTEWQTQLR+EIK
Sbjct: 297 LKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIK 356

Query: 301 QVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMW 360
           +VLGDK +DFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMW
Sbjct: 357 EVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMW 416

Query: 361 IDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEY 420
           IDVVAMHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEY
Sbjct: 417 IDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEY 476

Query: 421 KIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           KIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLL+H
Sbjct: 477 KIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 517

[Close]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL
Match: A0A6J1K6M8 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.2e-234
Identity = 400/452 (88.50%), Postives = 428/452 (94.69%), Query Frame = 0


Query: 16  LTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKPSVF 75
           LTH+IHSIVFPYFSRWQ A+GKIFTYWLGTEPFLY+ADPEF+K+LS EV+AK+WGKPSVF
Sbjct: 69  LTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKPSVF 128

Query: 76  KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVLS 135
           KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVESTT ML+RWA+LVLS
Sbjct: 129 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 188

Query: 136 GHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVL 195
           GHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTLFKTNRLVGVPFAG+L
Sbjct: 189 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGIL 248

Query: 196 NAGKAREARRLGEEIDGLFRAVITARRENQ---AAAAVLQQHDLLSLLLKESGEEGKLGR 255
           NAGKAREA+RLGEEID LFR VITARRENQ   AAA V+ Q+DLLSLLLKESG EGKL R
Sbjct: 249 NAGKAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGKLRR 308

Query: 256 KLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDFDF 315
            LST ELIDECKTFFFGGHETTALALTWTLLLL IHTEWQ QLR+EIK+VLGDK +DFDF
Sbjct: 309 WLSTAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESDFDF 368

Query: 316 TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQAL 375
           TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMWIDVVAMHHD+AL
Sbjct: 369 TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEAL 428

Query: 376 WGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFS 435
           WG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL RFS
Sbjct: 429 WGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFS 488

Query: 436 FSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           F+LSPDYCHSP IMLSLRPAHGLPLIF+LL++
Sbjct: 489 FTLSPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

[Close]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL
Match: A0A6J1E0B2 (cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 379/463 (81.86%), Postives = 416/463 (89.85%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKIL 60
           MK+ N    + ESSQ+THNIHS VFPYFSRWQ +HGKIF YWLGTEPFLYIA+PEFLK L
Sbjct: 56  MKKKNKNCSVLESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTL 115

Query: 61  STEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVE 120
           STEVKAKFWGKPSVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVE
Sbjct: 116 STEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVE 175

Query: 121 STTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTL 180
           STT MLERWA ++ SG  +IEVE+EIT+TAGEIIAKTSFGIDH+SGRQVF+KLR LQMTL
Sbjct: 176 STTEMLERWARMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFEKLRALQMTL 235

Query: 181 FKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLL 240
           FKTNRLVGVPF  +LNAGKAREA RLGEEIDGLF +VITARRE         Q DLLSLL
Sbjct: 236 FKTNRLVGVPFGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQV---QQDLLSLL 295

Query: 241 LKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIK 300
           L++ G     GR+LSTTEL+DECKTFFFGGHETTALA+TWTLLLL IHTEWQTQLREE+K
Sbjct: 296 LRDDG-----GRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVK 355

Query: 301 QVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMW 360
           +VLG+K ++FDF+ L+ LKKMGWVM EVLRLYPSAPNVQRQAR+DITINGLTIP+GTNMW
Sbjct: 356 EVLGNKESEFDFSNLAGLKKMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMW 415

Query: 361 IDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEY 420
           IDVVAMHHD+ALWGD+VNEFHPERF+NDA+AGGC+HKMGYLPFGFGGRMCVGRHLTFMEY
Sbjct: 416 IDVVAMHHDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEY 475

Query: 421 KIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQ 464
           KI++ LIL RFSFSLSP Y HSPSIMLSLRPAHGLPLIFQLL+
Sbjct: 476 KIMVALILCRFSFSLSPAYRHSPSIMLSLRPAHGLPLIFQLLE 510

[Close]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL
Match: E5GCU7 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.3e-219
Identity = 385/476 (80.88%), Postives = 420/476 (88.24%), Query Frame = 0


Query: 1   MKRNNLITP--IFESS------QLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIA 60
           MK  N+  P   FE S       +THNIHS VFPYF+RWQT +GK+FTYWLGTEPFLYIA
Sbjct: 36  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 95

Query: 61  DPEFLKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLK 120
           DPEF+KILS EVKAK WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLK
Sbjct: 96  DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 155

Query: 121 AMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQK 180
           AM+SLMVES T M+ERWANLV+SG+PQIEVE+EITSTAGEIIAKTSFGI H+SGRQVF K
Sbjct: 156 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 215

Query: 181 LRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAA--AAV 240
           LR LQ+TLFKTNRLVGVPF G+LNA KAREA+ LGEEID LFR+VIT RRE+  A     
Sbjct: 216 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTT 275

Query: 241 LQQHDLLSLLLKES--GEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIH 300
            QQ+DLLSLLLKES  G  G+ GR+LST ELIDECKTFFFGGHETTALALTWTL+LLA+H
Sbjct: 276 EQQNDLLSLLLKESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 335

Query: 301 TEWQTQLREEIKQVLG-DKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT 360
           +EWQT LREEIK+V G +  T FDFT LS LKKMGWVMSEVLRLYPSAPNVQRQAR+DI 
Sbjct: 336 SEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDII 395

Query: 361 INGLTIPNGTNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGG 420
           INGLTIPNGTNMWIDVV+MHHDQALWG QVNEFHPERF+ND ++GGC+HKMGYLPFGFGG
Sbjct: 396 INGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 455

Query: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQ 464
           RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDY HSPSIMLSLRPAHGLPL+F+LL+
Sbjct: 456 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLE 511

[Close]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL
Match: A0A1S4DS94 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.3e-219
Identity = 385/476 (80.88%), Postives = 420/476 (88.24%), Query Frame = 0


Query: 1   MKRNNLITP--IFESS------QLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIA 60
           MK  N+  P   FE S       +THNIHS VFPYF+RWQT +GK+FTYWLGTEPFLYIA
Sbjct: 63  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 122

Query: 61  DPEFLKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLK 120
           DPEF+KILS EVKAK WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLK
Sbjct: 123 DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 182

Query: 121 AMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQK 180
           AM+SLMVES T M+ERWANLV+SG+PQIEVE+EITSTAGEIIAKTSFGI H+SGRQVF K
Sbjct: 183 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 242

Query: 181 LRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAA--AAV 240
           LR LQ+TLFKTNRLVGVPF G+LNA KAREA+ LGEEID LFR+VIT RRE+  A     
Sbjct: 243 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTT 302

Query: 241 LQQHDLLSLLLKES--GEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIH 300
            QQ+DLLSLLLKES  G  G+ GR+LST ELIDECKTFFFGGHETTALALTWTL+LLA+H
Sbjct: 303 EQQNDLLSLLLKESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 362

Query: 301 TEWQTQLREEIKQVLG-DKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT 360
           +EWQT LREEIK+V G +  T FDFT LS LKKMGWVMSEVLRLYPSAPNVQRQAR+DI 
Sbjct: 363 SEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDII 422

Query: 361 INGLTIPNGTNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGG 420
           INGLTIPNGTNMWIDVV+MHHDQALWG QVNEFHPERF+ND ++GGC+HKMGYLPFGFGG
Sbjct: 423 INGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 482

Query: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQ 464
           RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDY HSPSIMLSLRPAHGLPL+F+LL+
Sbjct: 483 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLE 538

[Close]

BLAST of Lcy02g000210 vs. NCBI nr
Match: XP_022958036.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 842.4 bits (2175), Expect = 1.8e-240
Identity = 411/464 (88.58%), Postives = 440/464 (94.83%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKIL 60
           MKR +    +FES  LTH+IHSIVFPYFSRWQ AHGK+FTYWLGTEPFLY+A+PEF+K+L
Sbjct: 57  MKRTS-FDRVFES--LTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKML 116

Query: 61  STEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVE 120
           S EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVE
Sbjct: 117 SREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVE 176

Query: 121 STTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTL 180
           STT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTL
Sbjct: 177 STTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTL 236

Query: 181 FKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLL 240
           FKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQAAA V+QQ+DLLSLL
Sbjct: 237 FKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLL 296

Query: 241 LKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIK 300
           LKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLL IHTEWQTQLR+EIK
Sbjct: 297 LKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIK 356

Query: 301 QVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMW 360
           +VLGDK +DFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMW
Sbjct: 357 EVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMW 416

Query: 361 IDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEY 420
           IDVVAMHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEY
Sbjct: 417 IDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEY 476

Query: 421 KIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           KIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLL+H
Sbjct: 477 KIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 517

[Close]

BLAST of Lcy02g000210 vs. NCBI nr
Match: XP_023533013.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 834.3 bits (2154), Expect = 5.0e-238
Identity = 409/459 (89.11%), Postives = 436/459 (94.99%), Query Frame = 0


Query: 10  IFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFW 69
           +FES  LTH+IHSIVFPYFSRWQ AHGKIFTYWLGTEPFLYIA+PEF+++LS EV+AK+W
Sbjct: 54  VFES--LTHDIHSIVFPYFSRWQAAHGKIFTYWLGTEPFLYIAEPEFVRMLSREVQAKYW 113

Query: 70  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 129
           GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVESTT ML+RW
Sbjct: 114 GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRW 173

Query: 130 ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 189
           A+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTLFKTNRLVGV
Sbjct: 174 AHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGV 233

Query: 190 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAA----VLQQHDLLSLLLKESG 249
           PFAG+L AGKAREA+RLGEEID LFR VITARRENQAAAA    V+QQ+DLLSLLLKESG
Sbjct: 234 PFAGILIAGKAREAKRLGEEIDELFREVITARRENQAAAAAAAEVVQQNDLLSLLLKESG 293

Query: 250 EEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGD 309
            EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLL IHTEWQTQLR+EIK+VLGD
Sbjct: 294 GEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGD 353

Query: 310 KHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVA 369
           K +DFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMWIDVVA
Sbjct: 354 KESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVA 413

Query: 370 MHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLT 429
           MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLT
Sbjct: 414 MHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLT 473

Query: 430 LILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           LIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLL+H
Sbjct: 474 LILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 510

[Close]

BLAST of Lcy02g000210 vs. NCBI nr
Match: XP_022995689.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 819.7 bits (2116), Expect = 1.3e-233
Identity = 400/452 (88.50%), Postives = 428/452 (94.69%), Query Frame = 0


Query: 16  LTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKPSVF 75
           LTH+IHSIVFPYFSRWQ A+GKIFTYWLGTEPFLY+ADPEF+K+LS EV+AK+WGKPSVF
Sbjct: 69  LTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKPSVF 128

Query: 76  KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVLS 135
           KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVESTT ML+RWA+LVLS
Sbjct: 129 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 188

Query: 136 GHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVL 195
           GHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTLFKTNRLVGVPFAG+L
Sbjct: 189 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGIL 248

Query: 196 NAGKAREARRLGEEIDGLFRAVITARRENQ---AAAAVLQQHDLLSLLLKESGEEGKLGR 255
           NAGKAREA+RLGEEID LFR VITARRENQ   AAA V+ Q+DLLSLLLKESG EGKL R
Sbjct: 249 NAGKAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGKLRR 308

Query: 256 KLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDFDF 315
            LST ELIDECKTFFFGGHETTALALTWTLLLL IHTEWQ QLR+EIK+VLGDK +DFDF
Sbjct: 309 WLSTAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESDFDF 368

Query: 316 TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQAL 375
           TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMWIDVVAMHHD+AL
Sbjct: 369 TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEAL 428

Query: 376 WGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFS 435
           WG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL RFS
Sbjct: 429 WGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFS 488

Query: 436 FSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           F+LSPDYCHSP IMLSLRPAHGLPLIF+LL++
Sbjct: 489 FTLSPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

[Close]

BLAST of Lcy02g000210 vs. NCBI nr
Match: KAG7035636.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 394/455 (86.59%), Postives = 419/455 (92.09%), Query Frame = 0


Query: 10  IFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFW 69
           +FES  LTH+IHSIVFPYFSRWQ AH                 +PEF+K+LS EV+AK+W
Sbjct: 54  VFES--LTHDIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYW 113

Query: 70  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 129
           GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAM+SLMVESTT ML+RW
Sbjct: 114 GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRW 173

Query: 130 ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 189
           A+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTLFKTNRLVGV
Sbjct: 174 AHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGV 233

Query: 190 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLLLKESGEEGK 249
           PFAGVLNAGKAREA+RLGEEID LFR VITARRENQAAA V+QQ+DLLSLLLKESG EGK
Sbjct: 234 PFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGK 293

Query: 250 LGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTD 309
           LGR LSTTELIDECKTFFFGGHETTALALTWTLLLL IHTEWQTQLR+EIK+VLGDK +D
Sbjct: 294 LGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESD 353

Query: 310 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHD 369
           FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMWIDVVAMHHD
Sbjct: 354 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 413

Query: 370 QALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILS 429
           +ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 
Sbjct: 414 EALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 473

Query: 430 RFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLL+H
Sbjct: 474 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 489

[Close]

BLAST of Lcy02g000210 vs. NCBI nr
Match: KAG6605729.1 (Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793.1 bits (2047), Expect = 1.3e-225
Identity = 391/455 (85.93%), Postives = 417/455 (91.65%), Query Frame = 0


Query: 10  IFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFW 69
           +FES  LTH+IHSIVFPYFSRWQ AHGKIFTYWLGTEPFLY+A+PEF+K+LS EV+AK+W
Sbjct: 65  VFES--LTHDIHSIVFPYFSRWQAAHGKIFTYWLGTEPFLYVAEPEFVKMLSREVQAKYW 124

Query: 70  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 129
           GKPSVFKRDRKSMFGNGLVMAEGDEW                 AM+SLMVESTT ML+RW
Sbjct: 125 GKPSVFKRDRKSMFGNGLVMAEGDEW-----------------AMASLMVESTTQMLDRW 184

Query: 130 ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 189
           A LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KLR+LQMTLFKTNRLVGV
Sbjct: 185 AQLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGV 244

Query: 190 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLLLKESGEEGK 249
           PFAGVLNAGKAREA+RLGEEID LFR VITARRENQAAA V+QQ+DLLSLLLKESG EGK
Sbjct: 245 PFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGK 304

Query: 250 LGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTD 309
           LGR LSTTELIDECKTFFFGGHETTALALTWTLLLL IHTEWQ QLR+EIK+VLGDK +D
Sbjct: 305 LGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESD 364

Query: 310 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHD 369
           FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT+NGLTIPNGTNMWIDVVAMHHD
Sbjct: 365 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 424

Query: 370 QALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILS 429
           +ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 
Sbjct: 425 EALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 484

Query: 430 RFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLQH 465
           RFSF+LSPDYCHSP IMLSLRPAHGLPLIF+LL+H
Sbjct: 485 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLEH 500

[Close]

BLAST of Lcy02g000210 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 561.2 bits (1445), Expect = 7.6e-160
Identity = 273/466 (58.58%), Postives = 350/466 (75.11%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQ----LTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEF 60
           MK+  + + + ++S+    + H+IHSI  P+F+RWQ  +GK+F YWLG EPF+Y+ADPEF
Sbjct: 63  MKKLKMASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEF 122

Query: 61  LKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSS 120
           L ++S  V  K WGKP+VFK+DR+ MFG GLVM EGD+W R RH+ITPAF P NLK M++
Sbjct: 123 LSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTN 182

Query: 121 LMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDL 180
           +MVES ++ML+RW   + SG+P+ ++E EI  TAGEIIAKTSFG+  ++G QV + LR +
Sbjct: 183 MMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAV 242

Query: 181 QMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDL 240
           Q  LF +NR VGVPF+ +L+  +  +A+ LG EIDGL  + I  +R+   A    Q HDL
Sbjct: 243 QFALFNSNRYVGVPFSNILSYKQTVKAKGLGHEIDGLLLSFIN-KRKISLAEGDDQGHDL 302

Query: 241 LSLLLKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLR 300
           L +LLK + ++G    K    EL+DECKTFFF GHETTALALTWT +LLAIH EWQ  +R
Sbjct: 303 LGMLLK-ADQKGNFTAK----ELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIR 362

Query: 301 EEIKQVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNG 360
           EEI++V+GD  +  ++ KL+ LKKM WVM+EVLRLYP APN QRQAR DI +NG  IPNG
Sbjct: 363 EEIREVIGD--SKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNG 422

Query: 361 TNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLT 420
           TN+WIDVVAMHHD  LWGD VNEF PERF  + + GGC +KMGY+PFGFGGRMC+GR+LT
Sbjct: 423 TNIWIDVVAMHHDVELWGDDVNEFKPERFDGN-LHGGCKNKMGYMPFGFGGRMCIGRNLT 482

Query: 421 FMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
            MEYKIVL+L+LSRF  S+SP Y HSP+ MLSLRP +GLPLI + L
Sbjct: 483 TMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

[Close]

BLAST of Lcy02g000210 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 292.7 bits (748), Expect = 5.1e-79
Identity = 161/456 (35.31%), Postives = 257/456 (56.36%), Query Frame = 0


Query: 12  ESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTE---VKAKF 71
           +   + H+I   + P++  W   +GK F  W GT+P L + + E +K L  +   V  + 
Sbjct: 70  DCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRS 129

Query: 72  WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLER 131
           W    + ++  K+  G GL+MA G +W  QRH+  PAF    LK  +  MVE T+ ++ER
Sbjct: 130 W----LQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 189

Query: 132 WANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVG 191
               V  G  ++E+ +E+     +II++T FG   + G+++F  L  LQ    +  R + 
Sbjct: 190 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 249

Query: 192 VPFAGVLNAGKAREARRLGEEIDGLFRAVITARRE-NQAAAAVLQQHDLLSLLLKESGEE 251
            P +  L +   RE + L +E++ L   +I +RR+  +   +     DLL LLL E   +
Sbjct: 250 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 309

Query: 252 GKLGRKLSTTELI-DECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDK 311
                  +  +LI DECKTFFF GHETTAL LTWT +LLA +  WQ ++REE+++V G  
Sbjct: 310 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 369

Query: 312 HTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAM 371
                  +LS+L  +  V++E LRLYP A  + R A  D+ +  LTIP G ++WI V+A+
Sbjct: 370 GLP-SVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAI 429

Query: 372 HHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTL 431
           HH + LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI+L  
Sbjct: 430 HHSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILAT 489

Query: 432 ILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
           ++S+F+F++S +Y H+P ++L+++P +G+ +I + L
Sbjct: 490 LISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lcy02g000210 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 290.4 bits (742), Expect = 2.5e-78
Identity = 163/452 (36.06%), Postives = 251/452 (55.53%), Query Frame = 0


Query: 12  ESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGK 71
           + S + HNI   + P++  W   +GK F  W GTEP L + + E +K L T+      GK
Sbjct: 71  DCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK-HNPVTGK 130

Query: 72  PSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWAN 131
             + ++  K   G GL+MA G+ W  QRH+  PAF    LK  +  MVE T  M ER   
Sbjct: 131 SWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRK 190

Query: 132 LVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPF 191
            V     ++E+ +E+     +II++T FG     G+++F  L  LQ    +  R +  P 
Sbjct: 191 EV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG 250

Query: 192 AGVLNAGKAREARRLGEEIDGLFRAVITARREN-QAAAAVLQQHDLLSLLLKESGEEGKL 251
           +  L +   RE + L  E++ L   +I +R+++ +   +     DLL LLL +       
Sbjct: 251 SRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN--- 310

Query: 252 GRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKHTDF 311
              L+   ++DECKTFFF GHETT+L LTWTL+LLA +  WQ  +R+E++QV G      
Sbjct: 311 KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVP- 370

Query: 312 DFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMHHDQ 371
              +LS L  +  V++E LRLYP A  + R A  DI +  L IP G ++WI V+A+HH  
Sbjct: 371 SVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSN 430

Query: 372 ALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSR 431
            LWG+  NEF+PERF   + A        ++PF  G R C+G+    ME KI+L +++S+
Sbjct: 431 ELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSK 490

Query: 432 FSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 463
           FSF++S +Y H+P ++L+++P +G+ L+ + L
Sbjct: 491 FSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of Lcy02g000210 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 285.8 bits (730), Expect = 6.2e-77
Identity = 168/469 (35.82%), Postives = 264/469 (56.29%), Query Frame = 0


Query: 1   MKRNNLITPIFESSQLTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKIL 60
           MKR + ++ +  SS   ++I   + P++ +W + +G+ F YW GTEP + I+DPE  K +
Sbjct: 61  MKRESHLSILDPSS---NDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTM 120

Query: 61  STEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVE 120
            +  K  F+ K        K +   GLV  EG +WVR R ++ PAF+   LK M+++MV+
Sbjct: 121 LSN-KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVD 180

Query: 121 STTHMLERW---ANLVLSGHPQI--EVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRD 180
            T  MLE W   +    + HP+I  E+ +E      +IIA ++FG  +  G +VF+   +
Sbjct: 181 CTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQME 240

Query: 181 LQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHD 240
           L+     +   V +P    L         +L  ++D   + +I++R ++++        D
Sbjct: 241 LKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKSDYG----DD 300

Query: 241 LLSLLLKESGEEGKLGRKLSTTELIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQL 300
           LL +LLK    EGK  RK+S  E+I EC+TFFFGGHETT+  L WT +LL++H +WQ +L
Sbjct: 301 LLGILLKAYNTEGK-ERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKL 360

Query: 301 REEIKQVLGDKHTDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPN 360
           REEI +  G + T  D    S+LK M  V+ E LRLY     + R+A  +I +  L IP 
Sbjct: 361 REEIFKECGKEKTP-DSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 420

Query: 361 GTNMWIDVVAMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHL 420
           GT + I ++ MH D+ LWG   ++F+P RF N  ++   +H    L F  G R C+G++ 
Sbjct: 421 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFAN-GVSRAANHPNALLAFSVGPRACIGQNF 480

Query: 421 TFMEYKIVLTLILSRFSF-SLSPDYCHSPSIMLSLRPAHGLPLIFQLLQ 464
             +E K VLT+IL RF F SL  +Y H+P   ++++P +GLP++ Q L+
Sbjct: 481 VMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLE 518

[Close]

BLAST of Lcy02g000210 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-74
Identity = 148/448 (33.04%), Postives = 247/448 (55.13%), Query Frame = 0


Query: 16  LTHNIHSIVFPYFSRWQTAHGKIFTYWLGTEPFLYIADPEFLKILSTEVKAKFWGKPSVF 75
           + H+  S +FPY   W+  +G+++TY  G +  LY+  PE +K L+ +      GK S  
Sbjct: 82  IAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELN-QANTLNLGKVSYV 141

Query: 76  KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVLS 135
            +  KS+ G G++ + G  W  QR +I P F    +K M  L+VES   ML +W  ++  
Sbjct: 142 TKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKR 201

Query: 136 GHP---QIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV-PF 195
                  I V++++ + + ++I++  FG     G+++F KLR LQ  +   N L  +  F
Sbjct: 202 EGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGF 261

Query: 196 AGVLNAGKAREARRLGEEIDGLFRAVITARRENQAAAAVLQQHDLLSLLLK--ESGEEGK 255
             V+   K     ++ E    +   +    +E +       + DL+ L+L+   S  +G 
Sbjct: 262 TDVVFGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDGN 321

Query: 256 LGRKLSTTE--LIDECKTFFFGGHETTALALTWTLLLLAIHTEWQTQLREEIKQVLGDKH 315
           L  K  + +  ++D CK+ +F GHET+A+A++W L+LLA++  WQT++R+E+   L  K+
Sbjct: 322 LEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEV--FLHCKN 381

Query: 316 TDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITINGLTIPNGTNMWIDVVAMH 375
              D   +S LK +  V+ E LRLYP A  V R+A  D  +  L +P G  +W  +  +H
Sbjct: 382 GIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLH 441

Query: 376 HDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI 435
            D  +WG   NEF+PERF ++ ++  C H   ++PFG G R+C+G++   ME K++++LI
Sbjct: 442 RDPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLI 501

Query: 436 LSRFSFSLSPDYCHSPSIMLSLRPAHGL 456
           +SRFSF+LSP Y HSP   + + P HG+
Sbjct: 502 VSRFSFTLSPTYQHSPVFRMLVEPQHGV 525

The following BLAST results are available for this feature:

BLAST of Lcy02g000210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QYH7	2.0e-80	36.06	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
Q9FF18	7.1e-78	35.31	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q5KQH7	1.2e-77	34.07	Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ...	[more]
Q9ZW95	3.5e-77	36.06	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q9ASR3	8.7e-76	35.82	Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1	[more]

BLAST of Lcy02g000210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H0W5	8.9e-241	88.58	cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV...	[more]
A0A6J1K6M8	6.2e-234	88.50	cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1	[more]
A0A6J1E0B2	2.1e-221	81.86	cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 S...	[more]
E5GCU7	7.3e-219	80.88	Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S4DS94	7.3e-219	80.88	cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1	[more]

BLAST of Lcy02g000210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958036.1	1.8e-240	88.58	cytokinin hydroxylase-like [Cucurbita moschata]	[more]
XP_023533013.1	5.0e-238	89.11	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022995689.1	1.3e-233	88.50	cytokinin hydroxylase-like [Cucurbita maxima]	[more]
KAG7035636.1	2.6e-226	86.59	Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605729.1	1.3e-225	85.93	Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lcy02g000210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	7.6e-160	58.58	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G38450.1	5.1e-79	35.31	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	2.5e-78	36.06	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT2G46960.2	6.2e-77	35.82	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]
AT5G24910.1	1.7e-74	33.04	cytochrome P450, family 714, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 323..334 score: 39.82 coord: 269..286 score: 41.07 coord: 401..410 score: 57.77 coord: 410..421 score: 34.73
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 19..441 e-value: 2.3E-82 score: 277.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 410..433 score: 32.68 coord: 278..304 score: 34.86 coord: 400..410 score: 51.95 coord: 32..51 score: 24.77 coord: 258..275 score: 27.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 7..462 e-value: 8.1E-111 score: 372.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 10..459
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 1..458
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 1..458
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 403..412

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g000210.1	Lcy02g000210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen