Homology
BLAST of Lcy01g018100 vs. ExPASy Swiss-Prot
Match:
O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)
HSP 1 Score: 675.6 bits (1742), Expect = 6.4e-193
Identity = 363/754 (48.14%), Postives = 501/754 (66.45%), Query Frame = 0
Query: 12 ETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKH 71
+ YIVH+D + MP F HH W+S+T+ S+ ++ SS + KL++SY +
Sbjct: 23 QRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI-----KASVPSSVDRFHSAPKLVYSYDN 82
Query: 72 AINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSD 131
++GFSA L+ EL AL+K PG++S+ +D +V+ TTH+S FL L+ +SGL P S G D
Sbjct: 83 VLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLGQD 142
Query: 132 VIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIA 191
VI+ V+D+G+WPES SF DDGM EIP RWKG C+ G F S+CN+KLIGA +FNKG++A
Sbjct: 143 VIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGILA 202
Query: 192 KFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVW 251
P + I+MNS RDT+GHGTH ++ AG++ K S FGY G ARGVAPRAR+A+YK +
Sbjct: 203 NDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKFSF 262
Query: 252 AEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGN 311
EG + SD+IAA+D A+ DGVD+IS+S G +PLYED I++A++ AM +G+ V+ SAGN
Sbjct: 263 NEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASAGN 322
Query: 312 NGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVY 371
GP +G+++NG+PW+L VA+G DR F GT+TL NG+ + G SLFP + + S P++Y
Sbjct: 323 RGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDS--PVIY 382
Query: 372 ---MGRCHNSKKLKRV---GYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISDWDN 431
+ C + + L +V IV+C+D + S +++ + A++ +FIS
Sbjct: 383 NKTLSDCSSEELLSQVENPENTIVICDDNGDFSDQMRI--ITRARLKAAIFISEDPGVFR 442
Query: 432 LIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPSESC 491
P P + ++ G + +Y+K S P A + F +T L TKPAP VA S+RGPS S
Sbjct: 443 SATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSY 502
Query: 492 PFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVL-SGTSMSCPHAAGVAALLKG 551
+ KPDI+APG ILA++P NV AT + + + S +L SGTSM+ PHAAG+AA+LK
Sbjct: 503 LGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKA 562
Query: 552 AHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLVY 611
AHP WS +AIRSAMMTTAD LDNTR PIKD D NK ATPL MG+GHV+PN+A+DPGLVY
Sbjct: 563 AHPEWSPSAIRSAMMTTADPLDNTRKPIKD-SDNNKAATPLDMGAGHVDPNRALDPGLVY 622
Query: 612 DMGIQDYVNLLCALNYTKNQIQTITRST-SNNCENPSLDLNYPSFIIIVNASDSKATREI 671
D QDYVNLLC+LN+T+ Q +TI RS+ S+NC NPS DLNYPSFI + + + E
Sbjct: 623 DATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFTLLE- 682
Query: 672 SREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKI-----EGR 731
++F R VTN+G+ TYKAKL A ++V P+ L FK KN+K S+ L I EG+
Sbjct: 683 -QKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQ 742
Query: 732 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
+R G ++WVE G H V+SPIV + +
Sbjct: 743 SR-------NVGSITWVEQNGNHSVRSPIVTSPI 757
BLAST of Lcy01g018100 vs. ExPASy Swiss-Prot
Match:
Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)
HSP 1 Score: 593.6 bits (1529), Expect = 3.2e-168
Identity = 337/766 (43.99%), Postives = 471/766 (61.49%), Query Frame = 0
Query: 4 FWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSS 63
F++ ET YI+HMDL+A P PF+ H SW+S TL+SV+ N +
Sbjct: 13 FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK--------------P 72
Query: 64 KLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLL 123
K+I++Y +++GFSA LT SEL+ L+ PGYVS +D V++ TT S F+GL+S SG
Sbjct: 73 KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTW 132
Query: 124 PISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGAR 183
P+SNYG+ ++IG++DTG+WP+S SF+DDG+ +PS+WKG CE + SLCNKKLIGA+
Sbjct: 133 PVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNS---SSLCNKKLIGAK 192
Query: 184 FFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 243
FNKGL A P++ + +S DT GHGTH + AAG++VK AS+F Y G A G+A
Sbjct: 193 VFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIA 252
Query: 244 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------NGVPLYEDPI 303
P A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+ +G L DPI
Sbjct: 253 PHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPI 312
Query: 304 AVATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVL 363
AVA++AA+++G+FV TS GN+GP ++ NGAPW++ V AGT+ R F GT+T N VS
Sbjct: 313 AVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFS 372
Query: 364 GSSLFPLNSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKV 423
SLFP P+ + G N K + +IVVC + ++ ++ ++S
Sbjct: 373 FPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGA 432
Query: 424 AVGVFISN-ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGS-SDPKARVNFHKTILGTKP 483
A V I++ + + + I+ FP F+ I+ Y + ++ A++ F KT++GTKP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492
Query: 484 APSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTS 543
AP V YSSRGP S P +LKPDI+APG IL++WP T + P++S FN+L+GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552
Query: 544 MSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGS 603
M+ PH AGVAAL+K HP WS +AI+SA+MTTA LDN PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612
Query: 604 GHVNPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPS 663
GHV+ NK ++PGL+YD QD++N LC ++ I ITRS S+ C+ PS LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672
Query: 664 FIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKND 723
I + S + F R +TN+GE +Y ++ + GL V V+PKKL F KN+
Sbjct: 673 IIAYFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732
Query: 724 KLSFKLKIEGRARVKRETDVVFGYLSWV-EVGGKHIVQSPIVVTSL 753
KLS+ +++E + + +VV+G +SWV E + V +V TSL
Sbjct: 733 KLSYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVATSL 732
BLAST of Lcy01g018100 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 545.0 bits (1403), Expect = 1.3e-153
Identity = 315/757 (41.61%), Postives = 443/757 (58.52%), Query Frame = 0
Query: 11 TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYK 70
T + YI+ ++ + P+ F +HH WY++ L+S S L+++Y
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------ESSLLYTYT 84
Query: 71 HAINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYG 130
+ +GFSA L +E ++L S + + D + TT + FLGL+S G+ + +
Sbjct: 85 TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 144
Query: 131 SDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGL 190
+ VIIGV+DTGVWPES SF+D M EIPS+WKGECESG+ F LCNKKLIGAR F+KG
Sbjct: 145 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 204
Query: 191 -IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAI 250
+A + S RD +GHGTHTSTTAAGS V+ ASF GY +G ARG+A RARVA
Sbjct: 205 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 264
Query: 251 YKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVA 310
YK W+ G + SD++AA+D AILDGVDV+SLSLG P Y D IA+ ++AMERG+FV+
Sbjct: 265 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 324
Query: 311 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSP 370
SAGN+GP +V N APWV+ V AGT+DRDF L NG + G SL+ M P
Sbjct: 325 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKP 384
Query: 371 LPIVYMGRCHNSKKLKRVGY--------KIVVCEDKDEHSLSLQVDNVQSAKVAVG---V 430
L +VY +S L G KIVVC + ++ +V+ + A G +
Sbjct: 385 LELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEKGAVVRDAGGLGMI 444
Query: 431 FISNISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSV 490
+ + + L+ P+I + G+++++Y+K S P A + F T+L KP+P V
Sbjct: 445 MANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVV 504
Query: 491 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 550
A +SSRGP+ P +LKPD++ PG ILA W + T ++ ++FN++SGTSMSCP
Sbjct: 505 AAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCP 564
Query: 551 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVN 610
H +G+A LLK AHP WS +AI+SA+MTTA VLDNT P+ D D N L+ P A GSGHV+
Sbjct: 565 HISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVD 624
Query: 611 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFII 670
P KA+ PGLVYD+ ++Y+ LC+L+YT + I I + S NC D LNYPSF +
Sbjct: 625 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 684
Query: 671 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 730
+ + + R +TR VTN+G + YK + + ++VKP KL FK +K
Sbjct: 685 LFGG------KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 744
Query: 731 FKLKIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPI 748
+ + + V FG ++W +H V+SP+
Sbjct: 745 YTVTFVSKKGVSMTNKAEFGSITW--SNPQHEVRSPV 747
BLAST of Lcy01g018100 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 526.9 bits (1356), Expect = 3.7e-148
Identity = 314/757 (41.48%), Postives = 449/757 (59.31%), Query Frame = 0
Query: 16 YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
YIVHM + MP F H +WY ++L S+ D S++L+++Y++AI+G
Sbjct: 32 YIVHMAKSQMPSSFDLHSNWYDSSLRSISD----------------SAELLYTYENAIHG 91
Query: 76 FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVII 135
FS LT E ++L PG +S + + ++ TT + FLGL ++ L P + SDV++
Sbjct: 92 FSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVV 151
Query: 136 GVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKFP 195
GV+DTGVWPES S++D+G IPS WKG CE+G +F SLCN+KLIGARFF +G +
Sbjct: 152 GVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMG 211
Query: 196 NITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVWA 255
I S S RD +GHGTHTS+TAAGS V+ AS GY SG ARG+APRARVA+YK W
Sbjct: 212 PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL 271
Query: 256 EGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGNN 315
G + SD++AAID AI D V+V+S+SLG Y D +A+ +AAMERGI V+ SAGN
Sbjct: 272 GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNA 331
Query: 316 GPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVYM 375
GP ++ N APW+ V AGT+DRDF L NG + G SLF A+ LP +Y
Sbjct: 332 GPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALPDKLLPFIYA 391
Query: 376 GRCHNSKK----------LKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN-IS 435
G N+ ++V KIV+C+ + +Q +V A VG+ ++N +
Sbjct: 392 GNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAA 451
Query: 436 DWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 495
+ + L+ P+ + G+II+ Y+ +P A ++ T++G KP+P VA +SSR
Sbjct: 452 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSR 511
Query: 496 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 555
GP+ P +LKPD++APG ILA+W T + S +FN++SGTSMSCPH +G+A
Sbjct: 512 GPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLA 571
Query: 556 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 615
ALLK HP WS AAIRSA+MTTA P+ D+ K +TP G+GHV+P A +
Sbjct: 572 ALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATN 631
Query: 616 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNA 675
PGL+YD+ +DY+ LCALNYT QI++++R N +PS DLNYPSF + V+
Sbjct: 632 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDG 691
Query: 676 SDSKATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL 735
+ ++TR VT++G G TY K+ + G++++V+P L FK N+K S+ +
Sbjct: 692 VG-------AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 751
Query: 736 KIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVT 751
+ K FG + W + GKH+V SP+ ++
Sbjct: 752 TFTVDSS-KPSGSNSFGSIEWSD--GKHVVGSPVAIS 755
BLAST of Lcy01g018100 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 523.1 bits (1346), Expect = 5.3e-147
Identity = 319/766 (41.64%), Postives = 443/766 (57.83%), Query Frame = 0
Query: 14 ENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAI 73
+ +I +D +MP F +H+ WYS + S+++H Y
Sbjct: 26 KTFIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVF 85
Query: 74 NGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSDVI 133
+GFSA +TP E + L+ P ++ D ++ TT S FLGL + GL S+YGSDVI
Sbjct: 86 HGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVI 145
Query: 134 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKF 193
IGV DTG+WPE SF+D + IP RW+G CESGA F CN+K+IGARFF KG A
Sbjct: 146 IGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV 205
Query: 194 ---PNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAV 253
N T+ S RD +GHGTHTS+TAAG + +AS GY SG+A+GVAP+AR+A YK
Sbjct: 206 IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVC 265
Query: 254 WAE-GNYVSDVIAAIDHAILDGVDVISLSL-GVNGV--PLYEDPIAVATYAAMERGIFVA 313
W + G SD++AA D A+ DGVDVIS+S+ G +G+ P Y DPIA+ +Y A +GIFV+
Sbjct: 266 WKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVS 325
Query: 314 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF---PLNSAMT 373
+SAGN GP +V N APWV V A T+DR+F L +G + G SL+ PLN M
Sbjct: 326 SSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM- 385
Query: 374 VSPLPIVYMGR---------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVG 433
P+VY G+ N+ K+V KIV+C+ ++ + V VG
Sbjct: 386 ---FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGL--VVKKAGGVG 445
Query: 434 VFISN-ISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAP 493
+ ++N S+ + L+ P+ + G+ IK Y +P A ++F TI+G KPAP
Sbjct: 446 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 505
Query: 494 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMS 553
+A +S RGP+ P +LKPD++APG ILA+W V T + S P ++FN+LSGTSM+
Sbjct: 506 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 565
Query: 554 CPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGH 613
CPH +G AALLK AHP WS A IRSAMMTT +++DN+ + D K ATP GSGH
Sbjct: 566 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGH 625
Query: 614 VNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSN--NCENPSL-DLNYPSF 673
+N +AM+PGLVYD+ DY+ LC++ Y IQ ITR+ PS +LNYPS
Sbjct: 626 LNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS- 685
Query: 674 IIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDK 733
I + +S+ R TN+G+ Y+A++ + G+ VTVKP +L F +
Sbjct: 686 --ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 745
Query: 734 LSFKLKIEGRAR--VKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
S+ + + R V ET VFG ++W + GGKH+V+SPIVVT +
Sbjct: 746 RSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761
BLAST of Lcy01g018100 vs. ExPASy TrEMBL
Match:
A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)
HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 689/763 (90.30%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T +SSSSS
Sbjct: 14 MVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT-TTSSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DTTHSS+FL LS N
Sbjct: 74 SLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFLALSPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 SAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE LDLNYPSFI+ VNASDS+
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTVNASDSQT 673
Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
R E+SREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN LSFKLKI G
Sbjct: 674 GRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
+KRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSIKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 774
BLAST of Lcy01g018100 vs. ExPASy TrEMBL
Match:
A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)
HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 628/756 (83.07%), Postives = 681/756 (90.08%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M I T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T ++SSSS
Sbjct: 14 MVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL L+ N
Sbjct: 74 SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALTPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFND+ +S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF LN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFVLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYI SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTADV+DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPSFII VNASDS++
Sbjct: 614 PGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVNASDSES 673
Query: 661 -TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
REISREF R VTNIGEKG TY+AK+ M GL VTV+PK LKFK KN LSFKLKI G
Sbjct: 674 GRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
VKRE+DVVFGYL+WVEVGG H VQSPIVV L+S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRS 767
BLAST of Lcy01g018100 vs. ExPASy TrEMBL
Match:
A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)
HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 595/752 (79.12%), Postives = 660/752 (87.77%), Query Frame = 0
Query: 3 IFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLS 62
IFWL TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S + +SSSSSSS
Sbjct: 14 IFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSFP 73
Query: 63 SKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGL 122
SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHFLGLSSN GL
Sbjct: 74 SKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGL 133
Query: 123 LPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGA 182
LPIS YGSDVIIG VDTG+WP+S SFNDDGMSEIPSRWKGECES HF S CN KLIGA
Sbjct: 134 LPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGA 193
Query: 183 RFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRA 242
RFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G ARGVAPRA
Sbjct: 194 RFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRA 253
Query: 243 RVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERG 302
RVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G++G+PLY DP+++AT+AA+ERG
Sbjct: 254 RVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERG 313
Query: 303 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAM 362
IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITL+NGVSVLGSSLFPLN+ M
Sbjct: 314 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTM 373
Query: 363 TVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISD 422
+SPLPIV+MG C N KKLKR+GYKIVVCED D +SL+ QVDNVQ+AKV +G+FISNI D
Sbjct: 374 GLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFD 433
Query: 423 WDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPS 482
WDNLIQTPFPSIF++PY GNIIKDYI SSDPKA VNFHKTILGTKPAP+VARYSSRGPS
Sbjct: 434 WDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPS 493
Query: 483 ESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALL 542
+SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCPHAAGVAALL
Sbjct: 494 QSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALL 553
Query: 543 KGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGL 602
KGAHP+WS AAIRSAMMTTADV+DNT+T IKD+G+ NK ATPLAMGSGHVNPNKA+DP L
Sbjct: 554 KGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDL 613
Query: 603 VYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSK-ATR 662
+YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N+SDSK R
Sbjct: 614 IYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKR 673
Query: 663 EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGRARV 722
+I EF R +T IGE TY+AKL M G +V VKP+ L+FK KN KLSF+LKI G A
Sbjct: 674 KILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSA-- 733
Query: 723 KRETDVVFGYLSWVEVGGKHIVQSPIVVTSLK 754
E++VVFGYLSW EVGG+HIVQSPIVV ++
Sbjct: 734 -EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 762
BLAST of Lcy01g018100 vs. ExPASy TrEMBL
Match:
A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)
HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 595/752 (79.12%), Postives = 660/752 (87.77%), Query Frame = 0
Query: 3 IFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLS 62
IFWL TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S + +SSSSSSS
Sbjct: 14 IFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSFP 73
Query: 63 SKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGL 122
SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHFLGLSSN GL
Sbjct: 74 SKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGL 133
Query: 123 LPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGA 182
LPIS YGSDVIIG VDTG+WP+S SFNDDGMSEIPSRWKGECES HF S CN KLIGA
Sbjct: 134 LPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGA 193
Query: 183 RFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRA 242
RFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G ARGVAPRA
Sbjct: 194 RFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRA 253
Query: 243 RVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERG 302
RVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G++G+PLY DP+++AT+AA+ERG
Sbjct: 254 RVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERG 313
Query: 303 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAM 362
IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITL+NGVSVLGSSLFPLN+ M
Sbjct: 314 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTM 373
Query: 363 TVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISD 422
+SPLPIV+MG C N KKLKR+GYKIVVCED D +SL+ QVDNVQ+AKV +G+FISNI D
Sbjct: 374 GLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFD 433
Query: 423 WDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPS 482
WDNLIQTPFPSIF++PY GNIIKDYI SSDPKA VNFHKTILGTKPAP+VARYSSRGPS
Sbjct: 434 WDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPS 493
Query: 483 ESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALL 542
+SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCPHAAGVAALL
Sbjct: 494 QSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALL 553
Query: 543 KGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGL 602
KGAHP+WS AAIRSAMMTTADV+DNT+T IKD+G+ NK ATPLAMGSGHVNPNKA+DP L
Sbjct: 554 KGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDL 613
Query: 603 VYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSK-ATR 662
+YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N+SDSK R
Sbjct: 614 IYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKR 673
Query: 663 EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGRARV 722
+I EF R +T IGE TY+AKL M G +V VKP+ L+FK KN KLSF+LKI G A
Sbjct: 674 KILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSA-- 733
Query: 723 KRETDVVFGYLSWVEVGGKHIVQSPIVVTSLK 754
E++VVFGYLSW EVGG+HIVQSPIVV ++
Sbjct: 734 -EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 762
BLAST of Lcy01g018100 vs. ExPASy TrEMBL
Match:
A0A6J1BPR9 (subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 PE=3 SV=1)
HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 603/768 (78.52%), Postives = 669/768 (87.11%), Query Frame = 0
Query: 1 MAIFW--LPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSS 60
+AIFW LP TET+NYIVHMDLAAMPKPFA+HH+WYSATLSSVLDN RLG
Sbjct: 18 IAIFWFLLPSASTETDNYIVHMDLAAMPKPFATHHTWYSATLSSVLDNPRLG-------- 77
Query: 61 SSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSS 120
L SKLIH+Y HAINGFSAS+TPSEL+ALQKSPGYVSSV DSS+QVDTTHSSHFLGLSS
Sbjct: 78 -MLPSKLIHTYNHAINGFSASITPSELKALQKSPGYVSSVPDSSLQVDTTHSSHFLGLSS 137
Query: 121 NSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKK 180
+SG+L +SNYGSDVIIG VDTGVW ES SF+D+GM EIPSRWKG+CESG H CNKK
Sbjct: 138 DSGVL-LSNYGSDVIIGFVDTGVWAESESFSDEGMPEIPSRWKGKCESGTH-----CNKK 197
Query: 181 LIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGV 240
LIGARFFN+GLIAKFPN+TISMN T DTNGHGTHT+ TAAG YVK+ASFFGYG G ARGV
Sbjct: 198 LIGARFFNRGLIAKFPNVTISMNYTNDTNGHGTHTAATAAGGYVKDASFFGYGRGTARGV 257
Query: 241 APRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAA 300
APRARVAIYKA+W EGNYVSDVIAAID AILD VDV+SLSLG++G LYEDP+A+AT+AA
Sbjct: 258 APRARVAIYKAIWEEGNYVSDVIAAIDQAILDSVDVLSLSLGLDGALLYEDPVAIATFAA 317
Query: 301 MERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPL 360
+ERGI V TSAGN GPQLGTVHNGAPWVLNVAAGTMDRDFGGTI L NGVSVLGSSLFPL
Sbjct: 318 VERGILVTTSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIRLGNGVSVLGSSLFPL 377
Query: 361 NSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDE---HSLSLQVDNVQSAKVAVGV 420
NS M S LP+V+MG C+N KKLK+VG+KIVVCEDKDE +SLSLQVDNV++AKV VGV
Sbjct: 378 NSGMATSKLPLVFMGGCYNLKKLKKVGHKIVVCEDKDEYNSYSLSLQVDNVETAKVGVGV 437
Query: 421 FISNISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVAR 480
FISN+SDWDN +QT FPSIFLSP GNIIK YI+ SS+PKARV+FHKTI G KPAPSVAR
Sbjct: 438 FISNLSDWDNSVQTSFPSIFLSPSNGNIIKHYIRTSSNPKARVSFHKTIFGRKPAPSVAR 497
Query: 481 YSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHA 540
YSSRGP+ESCPFVLKPDIMAPGDAILASWP VAATDVNSRPIYSKFNVLSGTSMSCPHA
Sbjct: 498 YSSRGPAESCPFVLKPDIMAPGDAILASWPLKVAATDVNSRPIYSKFNVLSGTSMSCPHA 557
Query: 541 AGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPN 600
AGVAALLK AHPRWS AAIRSAMMTTADV+DNT+TPIKDLG++NKLATPLAMGSGHVNPN
Sbjct: 558 AGVAALLKAAHPRWSPAAIRSAMMTTADVVDNTQTPIKDLGEKNKLATPLAMGSGHVNPN 617
Query: 601 KAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNAS 660
KA+DPGL+YDM IQDYVN+LCALNY KNQIQTITRS SNNC+NPSLDLNYPSFI+IVNAS
Sbjct: 618 KAVDPGLIYDMEIQDYVNVLCALNYNKNQIQTITRSASNNCKNPSLDLNYPSFIVIVNAS 677
Query: 661 DSKATREISREFTRMVTNIGEKGTTYKAKLIAM--DGLRVTVKPKKLKFKWKNDKLSFKL 720
DS EISREF R VTN+ E+ TTY+AKL M +G+RVTVKP KL+FK KN KLSF +
Sbjct: 678 DSNTEMEISREFKRTVTNVVEEATTYEAKLTPMNGNGVRVTVKPHKLEFKGKNHKLSFTV 737
Query: 721 KIE-GRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHR 761
K++ G V+R+ +VVF YLSWVEVGG H+ +SPIVVT L ++SH+
Sbjct: 738 KVQLGHWIVQRDPNVVFCYLSWVEVGGGHVARSPIVVTDLNFMLRSHQ 770
BLAST of Lcy01g018100 vs. NCBI nr
Match:
XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 631/756 (83.47%), Postives = 685/756 (90.61%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M IF T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T ++SSSS
Sbjct: 14 MVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74 SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVI+LSLG++ VPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
SA+ +SPLPI +MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN++KDYI SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YDMGI+DY NL+CALNYTKNQIQTITRSTSN+CE PSLDLNYPSFII VNASDS+
Sbjct: 614 PGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPSFIITVNASDSET 673
Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
R EISREF R VTNIGEKG TY+AK+ M GL VTV+PKKLKFK KN LSFKLKI G
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
VKRE+DVVFGYL+WVEVGG H VQSPIVVT++ S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVTNMSS 767
BLAST of Lcy01g018100 vs. NCBI nr
Match:
XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])
HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 689/763 (90.30%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T +SSSSS
Sbjct: 14 MVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT-TTSSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DTTHSS+FL LS N
Sbjct: 74 SLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFLALSPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 SAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE LDLNYPSFI+ VNASDS+
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTVNASDSQT 673
Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
R E+SREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN LSFKLKI G
Sbjct: 674 GRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
+KRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSIKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 774
BLAST of Lcy01g018100 vs. NCBI nr
Match:
KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1236.1 bits (3197), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 686/763 (89.91%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
+ IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T SSSSSS
Sbjct: 14 IVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74 --PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
A+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 LAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPSFII VNASDS+
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVNASDSQT 673
Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
R EISREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN LSFKLKI G
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
VKRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 773
BLAST of Lcy01g018100 vs. NCBI nr
Match:
KAG7029306.1 (Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 685/763 (89.78%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M IF T T+T+NYIVHMDLAAMP FA+HHSWYSATLSSV LD S L T SSSSSS
Sbjct: 14 MVIFLARSTLTQTDNYIVHMDLAAMPNSFATHHSWYSATLSSVLLDTSPLRTTTSSSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74 --PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
A+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 LAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPSFII VNASDS+
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVNASDSQT 673
Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
R EISREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN LSFKLKI G
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
VKRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 773
BLAST of Lcy01g018100 vs. NCBI nr
Match:
XP_022996869.1 (subtilisin-like protease SBT1.9 [Cucurbita maxima])
HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 628/756 (83.07%), Postives = 681/756 (90.08%), Query Frame = 0
Query: 1 MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
M I T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T ++SSSS
Sbjct: 14 MVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73
Query: 61 SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL L+ N
Sbjct: 74 SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALTPN 133
Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
SGLLPISNYGSDVIIG VDTGVWPES SFND+ +S+IPSRWKGECESG HF SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNASLCNKKL 193
Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253
Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313
Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF LN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFVLN 373
Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433
Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
ISDWDNLIQT FPSIFL+ Y GN+IKDYI SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493
Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
GPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553
Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
ALLKGAHP WS AAIRSAMMTTADV+DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613
Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
PGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPSFII VNASDS++
Sbjct: 614 PGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVNASDSES 673
Query: 661 -TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
REISREF R VTNIGEKG TY+AK+ M GL VTV+PK LKFK KN LSFKLKI G
Sbjct: 674 GRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFKLKIRGH 733
Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
VKRE+DVVFGYL+WVEVGG H VQSPIVV L+S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRS 767
BLAST of Lcy01g018100 vs. TAIR 10
Match:
AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 593.6 bits (1529), Expect = 2.3e-169
Identity = 337/766 (43.99%), Postives = 471/766 (61.49%), Query Frame = 0
Query: 4 FWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSS 63
F++ ET YI+HMDL+A P PF+ H SW+S TL+SV+ N +
Sbjct: 13 FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK--------------P 72
Query: 64 KLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLL 123
K+I++Y +++GFSA LT SEL+ L+ PGYVS +D V++ TT S F+GL+S SG
Sbjct: 73 KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTW 132
Query: 124 PISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGAR 183
P+SNYG+ ++IG++DTG+WP+S SF+DDG+ +PS+WKG CE + SLCNKKLIGA+
Sbjct: 133 PVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNS---SSLCNKKLIGAK 192
Query: 184 FFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 243
FNKGL A P++ + +S DT GHGTH + AAG++VK AS+F Y G A G+A
Sbjct: 193 VFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIA 252
Query: 244 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------NGVPLYEDPI 303
P A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+ +G L DPI
Sbjct: 253 PHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPI 312
Query: 304 AVATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVL 363
AVA++AA+++G+FV TS GN+GP ++ NGAPW++ V AGT+ R F GT+T N VS
Sbjct: 313 AVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFS 372
Query: 364 GSSLFPLNSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKV 423
SLFP P+ + G N K + +IVVC + ++ ++ ++S
Sbjct: 373 FPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGA 432
Query: 424 AVGVFISN-ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGS-SDPKARVNFHKTILGTKP 483
A V I++ + + + I+ FP F+ I+ Y + ++ A++ F KT++GTKP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492
Query: 484 APSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTS 543
AP V YSSRGP S P +LKPDI+APG IL++WP T + P++S FN+L+GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552
Query: 544 MSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGS 603
M+ PH AGVAAL+K HP WS +AI+SA+MTTA LDN PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612
Query: 604 GHVNPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPS 663
GHV+ NK ++PGL+YD QD++N LC ++ I ITRS S+ C+ PS LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672
Query: 664 FIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKND 723
I + S + F R +TN+GE +Y ++ + GL V V+PKKL F KN+
Sbjct: 673 IIAYFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732
Query: 724 KLSFKLKIEGRARVKRETDVVFGYLSWV-EVGGKHIVQSPIVVTSL 753
KLS+ +++E + + +VV+G +SWV E + V +V TSL
Sbjct: 733 KLSYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVATSL 732
BLAST of Lcy01g018100 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 545.0 bits (1403), Expect = 9.3e-155
Identity = 315/757 (41.61%), Postives = 443/757 (58.52%), Query Frame = 0
Query: 11 TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYK 70
T + YI+ ++ + P+ F +HH WY++ L+S S L+++Y
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------ESSLLYTYT 84
Query: 71 HAINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYG 130
+ +GFSA L +E ++L S + + D + TT + FLGL+S G+ + +
Sbjct: 85 TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 144
Query: 131 SDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGL 190
+ VIIGV+DTGVWPES SF+D M EIPS+WKGECESG+ F LCNKKLIGAR F+KG
Sbjct: 145 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 204
Query: 191 -IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAI 250
+A + S RD +GHGTHTSTTAAGS V+ ASF GY +G ARG+A RARVA
Sbjct: 205 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 264
Query: 251 YKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVA 310
YK W+ G + SD++AA+D AILDGVDV+SLSLG P Y D IA+ ++AMERG+FV+
Sbjct: 265 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 324
Query: 311 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSP 370
SAGN+GP +V N APWV+ V AGT+DRDF L NG + G SL+ M P
Sbjct: 325 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKP 384
Query: 371 LPIVYMGRCHNSKKLKRVGY--------KIVVCEDKDEHSLSLQVDNVQSAKVAVG---V 430
L +VY +S L G KIVVC + ++ +V+ + A G +
Sbjct: 385 LELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEKGAVVRDAGGLGMI 444
Query: 431 FISNISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSV 490
+ + + L+ P+I + G+++++Y+K S P A + F T+L KP+P V
Sbjct: 445 MANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVV 504
Query: 491 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 550
A +SSRGP+ P +LKPD++ PG ILA W + T ++ ++FN++SGTSMSCP
Sbjct: 505 AAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCP 564
Query: 551 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVN 610
H +G+A LLK AHP WS +AI+SA+MTTA VLDNT P+ D D N L+ P A GSGHV+
Sbjct: 565 HISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVD 624
Query: 611 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFII 670
P KA+ PGLVYD+ ++Y+ LC+L+YT + I I + S NC D LNYPSF +
Sbjct: 625 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 684
Query: 671 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 730
+ + + R +TR VTN+G + YK + + ++VKP KL FK +K
Sbjct: 685 LFGG------KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 744
Query: 731 FKLKIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPI 748
+ + + V FG ++W +H V+SP+
Sbjct: 745 YTVTFVSKKGVSMTNKAEFGSITW--SNPQHEVRSPV 747
BLAST of Lcy01g018100 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-149
Identity = 314/757 (41.48%), Postives = 449/757 (59.31%), Query Frame = 0
Query: 16 YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
YIVHM + MP F H +WY ++L S+ D S++L+++Y++AI+G
Sbjct: 32 YIVHMAKSQMPSSFDLHSNWYDSSLRSISD----------------SAELLYTYENAIHG 91
Query: 76 FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVII 135
FS LT E ++L PG +S + + ++ TT + FLGL ++ L P + SDV++
Sbjct: 92 FSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVV 151
Query: 136 GVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKFP 195
GV+DTGVWPES S++D+G IPS WKG CE+G +F SLCN+KLIGARFF +G +
Sbjct: 152 GVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMG 211
Query: 196 NITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVWA 255
I S S RD +GHGTHTS+TAAGS V+ AS GY SG ARG+APRARVA+YK W
Sbjct: 212 PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL 271
Query: 256 EGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGNN 315
G + SD++AAID AI D V+V+S+SLG Y D +A+ +AAMERGI V+ SAGN
Sbjct: 272 GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNA 331
Query: 316 GPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVYM 375
GP ++ N APW+ V AGT+DRDF L NG + G SLF A+ LP +Y
Sbjct: 332 GPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALPDKLLPFIYA 391
Query: 376 GRCHNSKK----------LKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN-IS 435
G N+ ++V KIV+C+ + +Q +V A VG+ ++N +
Sbjct: 392 GNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAA 451
Query: 436 DWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 495
+ + L+ P+ + G+II+ Y+ +P A ++ T++G KP+P VA +SSR
Sbjct: 452 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSR 511
Query: 496 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 555
GP+ P +LKPD++APG ILA+W T + S +FN++SGTSMSCPH +G+A
Sbjct: 512 GPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLA 571
Query: 556 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 615
ALLK HP WS AAIRSA+MTTA P+ D+ K +TP G+GHV+P A +
Sbjct: 572 ALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATN 631
Query: 616 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNA 675
PGL+YD+ +DY+ LCALNYT QI++++R N +PS DLNYPSF + V+
Sbjct: 632 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDG 691
Query: 676 SDSKATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL 735
+ ++TR VT++G G TY K+ + G++++V+P L FK N+K S+ +
Sbjct: 692 VG-------AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 751
Query: 736 KIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVT 751
+ K FG + W + GKH+V SP+ ++
Sbjct: 752 TFTVDSS-KPSGSNSFGSIEWSD--GKHVVGSPVAIS 755
BLAST of Lcy01g018100 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 523.1 bits (1346), Expect = 3.8e-148
Identity = 319/766 (41.64%), Postives = 443/766 (57.83%), Query Frame = 0
Query: 14 ENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAI 73
+ +I +D +MP F +H+ WYS + S+++H Y
Sbjct: 26 KTFIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVF 85
Query: 74 NGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSDVI 133
+GFSA +TP E + L+ P ++ D ++ TT S FLGL + GL S+YGSDVI
Sbjct: 86 HGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVI 145
Query: 134 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKF 193
IGV DTG+WPE SF+D + IP RW+G CESGA F CN+K+IGARFF KG A
Sbjct: 146 IGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV 205
Query: 194 ---PNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAV 253
N T+ S RD +GHGTHTS+TAAG + +AS GY SG+A+GVAP+AR+A YK
Sbjct: 206 IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVC 265
Query: 254 WAE-GNYVSDVIAAIDHAILDGVDVISLSL-GVNGV--PLYEDPIAVATYAAMERGIFVA 313
W + G SD++AA D A+ DGVDVIS+S+ G +G+ P Y DPIA+ +Y A +GIFV+
Sbjct: 266 WKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVS 325
Query: 314 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF---PLNSAMT 373
+SAGN GP +V N APWV V A T+DR+F L +G + G SL+ PLN M
Sbjct: 326 SSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM- 385
Query: 374 VSPLPIVYMGR---------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVG 433
P+VY G+ N+ K+V KIV+C+ ++ + V VG
Sbjct: 386 ---FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGL--VVKKAGGVG 445
Query: 434 VFISN-ISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAP 493
+ ++N S+ + L+ P+ + G+ IK Y +P A ++F TI+G KPAP
Sbjct: 446 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 505
Query: 494 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMS 553
+A +S RGP+ P +LKPD++APG ILA+W V T + S P ++FN+LSGTSM+
Sbjct: 506 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 565
Query: 554 CPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGH 613
CPH +G AALLK AHP WS A IRSAMMTT +++DN+ + D K ATP GSGH
Sbjct: 566 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGH 625
Query: 614 VNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSN--NCENPSL-DLNYPSF 673
+N +AM+PGLVYD+ DY+ LC++ Y IQ ITR+ PS +LNYPS
Sbjct: 626 LNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS- 685
Query: 674 IIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDK 733
I + +S+ R TN+G+ Y+A++ + G+ VTVKP +L F +
Sbjct: 686 --ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 745
Query: 734 LSFKLKIEGRAR--VKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
S+ + + R V ET VFG ++W + GGKH+V+SPIVVT +
Sbjct: 746 RSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761
BLAST of Lcy01g018100 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 517.3 bits (1331), Expect = 2.1e-146
Identity = 319/768 (41.54%), Postives = 446/768 (58.07%), Query Frame = 0
Query: 16 YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
YIVH+D A P F +H WY+++L+S+ +S +IH+Y +G
Sbjct: 28 YIVHVDHEAKPSIFPTHFHWYTSSLASL---------------TSSPPSIIHTYDTVFHG 87
Query: 76 FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSS--NSGLLPISNYGSDVI 135
FSA LT + L P +S + + + TT S FLGL S +GLL S++GSD++
Sbjct: 88 FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 147
Query: 136 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAK- 195
IGV+DTGVWPE SF+D G+ +P +WKG+C + F S CN+KL+GARFF G A
Sbjct: 148 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 207
Query: 196 -FPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVW 255
N T S RD++GHGTHT++ +AG YV AS GY G+A G+AP+AR+A YK W
Sbjct: 208 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 267
Query: 256 AEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGN 315
G Y SD++AA D A+ DGVDVISLS+G VP Y D IA+ + A++RGIFV+ SAGN
Sbjct: 268 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 327
Query: 316 NGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVY 375
GP TV N APW+ V AGT+DRDF + L NG + G S++ P+VY
Sbjct: 328 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 387
Query: 376 MGR------------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 435
G S V KIV+C D+ +S + + + V+ +G+ I+N
Sbjct: 388 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLC-DRGINSRATKGEIVRK-NGGLGMIIAN 447
Query: 436 -ISDWDNLIQT--PFPSIFLSPYRGNIIKDYI------KGSSDPKARVNFHKTILGTKPA 495
+ D + L+ P+ + G+ I+ YI + S P A + F T LG +PA
Sbjct: 448 GVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPA 507
Query: 496 PSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSM 555
P VA +S+RGP+ P +LKPD++APG ILA+WP + + V S ++FN+LSGTSM
Sbjct: 508 PVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSM 567
Query: 556 SCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSG 615
+CPH +G+AALLK AHP WS AAIRSA++TTA +DN+ P+ D N ++ + GSG
Sbjct: 568 ACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN-TSSVMDYGSG 627
Query: 616 HVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSL-----DLNY 675
HV+P KAMDPGLVYD+ DY+N LC NYT+ I TITR + +C+ +LNY
Sbjct: 628 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNY 687
Query: 676 PSFIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWK 735
PSF ++ ++S F R VTN+G+ + Y+ K+ G VTV+P+KL F+
Sbjct: 688 PSFSVVF---QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRV 747
Query: 736 NDKLSFKLKIEGRARVKRE---TDVVFGYLSWVEVGGKHIVQSPIVVT 751
KLSF ++++ VK T+V G++ W + GK V SP+VVT
Sbjct: 748 GQKLSFVVRVK-TTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVVT 770
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O82777 | 6.4e-193 | 48.14 | Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1 | [more] |
| Q9FHA4 | 3.2e-168 | 43.99 | Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 S... | [more] |
| Q9ZUF6 | 1.3e-153 | 41.61 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| O65351 | 3.7e-148 | 41.48 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| O49607 | 5.3e-147 | 41.64 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HH07 | 0.0e+00 | 82.57 | subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE... | [more] |
| A0A6J1K9W2 | 0.0e+00 | 83.07 | subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3... | [more] |
| A0A5A7V589 | 0.0e+00 | 79.12 | Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3BBC3 | 0.0e+00 | 79.12 | subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=... | [more] |
| A0A6J1BPR9 | 0.0e+00 | 78.52 | subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_023546329.1 | 0.0e+00 | 83.47 | subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022962414.1 | 0.0e+00 | 82.57 | subtilisin-like protease SBT1.9 [Cucurbita moschata] | [more] |
| KAG6598336.1 | 0.0e+00 | 82.57 | Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG7029306.1 | 0.0e+00 | 82.57 | Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosp... | [more] |
| XP_022996869.1 | 0.0e+00 | 83.07 | subtilisin-like protease SBT1.9 [Cucurbita maxima] | [more] |
