Lcy01g017010 (gene) Sponge gourd (P93075) v1

BLAST of Lcy01g017010 vs. ExPASy Swiss-Prot
Match: Q8GXE6 (Potassium channel AKT6 OS=Arabidopsis thaliana OX=3702 GN=AKT6 PE=1 SV=2)

HSP 1 Score: 1003.4 bits (2593), Expect = 1.6e-291
Identity = 513/870 (58.97%), Postives = 647/870 (74.37%), Query Frame = 0

Query: 19  RDQEREI-EQLSRDG--SQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETF 78
           +D E ++ + LSRDG  SQYSL+ G+LP LG N R +R V L   I+SPFD  YR WETF
Sbjct: 27  KDAEDDVADHLSRDGTMSQYSLSKGLLPSLGANNRSSRDVILPRFIVSPFDPRYRAWETF 86

Query: 79  LIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDE 138
           L+ LVLYTAW  PFEFGFL+     L+++DN+VNG FA+DIVLTFFVA+LDK TYLLVD+
Sbjct: 87  LVFLVLYTAWASPFEFGFLQKPRPPLSILDNIVNGFFAVDIVLTFFVAFLDKVTYLLVDD 146

Query: 139 PKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEK 198
           PK IA RYA TWL+ DV+S  P E+  S+L  S Q YG F+MLRLWRLRRVS+ FAR+EK
Sbjct: 147 PKRIAWRYASTWLIFDVVSTFPYEIFGSLLHESIQGYGIFSMLRLWRLRRVSNCFARLEK 206

Query: 199 DRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARY 258
           DR ++YFWVRC+KL+ V LF +H   C  Y I + + DP K ++ L  E++ +  +  RY
Sbjct: 207 DRKYSYFWVRCSKLLLVTLFVIHCGACFLYSIAAHYPDPSKTFMALTDENWKESPIAVRY 266

Query: 259 VTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTR 318
            TA+YWSI T +TTGYGD+H VN REMTFI+ YM F LGL AY+IGNMTNLVVH T RTR
Sbjct: 267 NTAMYWSITTFSTTGYGDIHGVNSREMTFILFYMVFNLGLSAYIIGNMTNLVVHVTGRTR 326

Query: 319 KFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAH 378
           KFRDTIQAAS F QRN LPVRLQ+QM+AHLCL++RTD EGLQQQE +DSLPKAIRSSI+H
Sbjct: 327 KFRDTIQAASGFGQRNNLPVRLQDQMVAHLCLRYRTDSEGLQQQEIIDSLPKAIRSSISH 386

Query: 379 HLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLI 438
           +LFY +VD  YLF+G+S DL+FQLVTEMKAEYFPPKED+ILQNEAP+DFYI+VTG  D+I
Sbjct: 387 YLFYEVVDKIYLFHGISNDLLFQLVTEMKAEYFPPKEDVILQNEAPTDFYILVTGAVDII 446

Query: 439 IQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGD 498
            + NG+E+++ EA+ G V GE+GVLCY+PQLFTVRT RLSQLLRLNR+   NLVQA+VGD
Sbjct: 447 ARVNGVEQVVSEAQRGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTVLLNLVQANVGD 506

Query: 499 GTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKR 558
           G IIM+NLL+HLK+ +DP+M+ ++ +T   L Q  M++PL+L +AA  GDDLLLHQLL+R
Sbjct: 507 GAIIMNNLLQHLKDSEDPVMKGVLADTEHMLAQGKMDLPLSLCFAAARGDDLLLHQLLRR 566

Query: 559 GSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESI 618
           GS PNE+D   G+TALH+AA++G+ +CVVLLLE+GADPN RD EGNVPLW+AI G+H  I
Sbjct: 567 GSSPNEMD-KDGRTALHIAASKGSHYCVVLLLEHGADPNIRDSEGNVPLWEAIIGRHREI 626

Query: 619 VKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEG 678
            KLL +N A +S   V+ F+  A E+N +D L+ I++ GGDVTL   NGTTALH AV EG
Sbjct: 627 AKLLAENGAKLSLDSVSYFSGLAVEKNCLDALKDIIKYGGDVTLPDGNGTTALHRAVSEG 686

Query: 679 NSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSM-NKPSSVVHIPQI 738
           + E+VKFLL+QGAD+D PD +GWTPR LADHQG+EEIK LF   + +  KP  +  IPQ 
Sbjct: 687 HLEIVKFLLDQGADLDWPDSYGWTPRGLADHQGNEEIKTLFHNHRPVEKKPKPIPGIPQS 746

Query: 739 PEPNY-LRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFMSTINNG 798
           P     L K+ SE  M      +S   V +     +  ++R+ NN++NSL G +S  N+ 
Sbjct: 747 PVTGKPLMKYSSEPTM------HSGELVLDGG-QVVVSQKRKLNNFRNSLFGIISAANSA 806

Query: 799 ERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFGI 858
           +     P S       G  + Y  RVT+S PE GE  GK+VLLP S++ELL+IG  K G 
Sbjct: 807 DDGGEVPRSPAVPGGGG-SMIYPERVTISSPENGETGGKVVLLPNSMEELLKIGENKMGF 866

Query: 859 SVTKILTQEGAEVEDVNLLRDGDRLVLVSD 884
             TK+LT+EGAE++D+ L+RDGD L+L  D
Sbjct: 867 VPTKVLTREGAEIDDITLIRDGDFLLLSRD 887

BLAST of Lcy01g017010 vs. ExPASy Swiss-Prot
Match: Q38998 (Potassium channel AKT1 OS=Arabidopsis thaliana OX=3702 GN=AKT1 PE=1 SV=2)

HSP 1 Score: 1000.7 bits (2586), Expect = 1.0e-290
Identity = 520/880 (59.09%), Postives = 658/880 (74.77%), Query Frame = 0

Query: 13  GISMCGRDQEREIEQLSRDGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLW 72
           G  +CG+ Q+ EIEQLSR+ S +SL+ GILP LG   R NRRVKLR  ++SP+D+ YR+W
Sbjct: 4   GALLCGQVQD-EIEQLSRESSHFSLSTGILPSLG--ARSNRRVKLRRFVVSPYDHKYRIW 63

Query: 73  ETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLL 132
           E FL+VLV+YTAWV PFEFGFL+     L++ DN+VN  FAIDI++TFFV YLDKSTYL+
Sbjct: 64  EAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLI 123

Query: 133 VDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFAR 192
           VD+ K IA +Y ++W +LD++S IPSE A  I   S+Q+YG FNMLRLWRLRRV ++FAR
Sbjct: 124 VDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRI---SSQSYGLFNMLRLWRLRRVGALFAR 183

Query: 193 MEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLW 252
           +EKDR+FNYFWVRCAKL+ V LF VH A C +YLI +   +P K W+     ++ +ESLW
Sbjct: 184 LEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLW 243

Query: 253 ARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTS 312
            RYVT++YWSI TLTT GYGDLH VN +EM F I YM F LGL AYLIGNMTNLVVHGTS
Sbjct: 244 MRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTS 303

Query: 313 RTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSS 372
           RTR FRDTIQAASNFA RN LP RLQ+QMLAHLCLK+RTD EGLQQQET+D+LPKAIRSS
Sbjct: 304 RTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSS 363

Query: 373 IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTA 432
           I+H LFYSL+D  YLF GVS DL+FQLV+EMKAEYFPPKED+ILQNEAP+DFYI+V GTA
Sbjct: 364 ISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTA 423

Query: 433 DLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQAS 492
           DL+    G E I+ E K GD+IGEIGVLCY+PQLFTVRT RL QLLR+NR++F N++QA+
Sbjct: 424 DLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQAN 483

Query: 493 VGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQL 552
           VGDGTIIM+NLL+HLKE+ DP+M  ++ E    L +  M++PLNL +AA   DDLLLHQL
Sbjct: 484 VGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQL 543

Query: 553 LKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKH 612
           LKRG  PNE D ++G+T LH+AA++G  +CV+LLLE  ADPN RD EG+VPLW+A+   H
Sbjct: 544 LKRGLDPNESD-NNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGH 603

Query: 613 ESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAV 672
           E +VK+L+++ + I +GDV  FAC AAEQ ++ +L+ IV  GGDVT  R+ GT+ALH AV
Sbjct: 604 EKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAV 663

Query: 673 CEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNK----PSSV 732
           CE N EMVK+LLEQGAD++K D+HGWTPR LA+ QGHE+IK LF  K    +     SS 
Sbjct: 664 CEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSS 723

Query: 733 VHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM- 792
           V I +     +L +F SE N+   S++ S   +RE+        RR++NN+ NSL G + 
Sbjct: 724 VPILK-TGIRFLGRFTSEPNIRPASREVSFR-IRETR------ARRKTNNFDNSLFGILA 783

Query: 793 --STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLE 852
             S   NG   V +  + N             RVT+SC EK ++AGKLVLLP S +ELLE
Sbjct: 784 NQSVPKNGLATVDEGRTGN-----------PVRVTISCAEKDDIAGKLVLLPGSFKELLE 843

Query: 853 IGAKKFGISVTKILTQE-GAEVEDVNLLRDGDRLVLVSDA 885
           +G+ KFGI  TK++ ++  AE++DV+++RDGD L+  +D+
Sbjct: 844 LGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS 857

BLAST of Lcy01g017010 vs. ExPASy Swiss-Prot
Match: Q9SCX5 (Probable potassium channel AKT5 OS=Arabidopsis thaliana OX=3702 GN=AKT5 PE=2 SV=2)

HSP 1 Score: 986.5 bits (2549), Expect = 2.0e-286
Identity = 502/860 (58.37%), Postives = 642/860 (74.65%), Query Frame = 0

Query: 26  EQLSRDG--SQYSLTGGILPPLGV--NGRKNRRVKLRPHIISPFDYHYRLWETFLIVLVL 85
           E +SR+G  S YS + G+LPPLGV    R +R +KLR  I+SPFD  YR W+ FL++LVL
Sbjct: 31  EHISREGTMSHYSFSKGLLPPLGVGATARSSRHIKLRCFIVSPFDPRYRAWDWFLVILVL 90

Query: 86  YTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEPKLIAM 145
           YTAW  PFEFGFL+T    L+++DNVVNG FA+DIVLTFFVA+LDK+TYLLVD+PK IA 
Sbjct: 91  YTAWASPFEFGFLQTPRAPLSILDNVVNGFFAVDIVLTFFVAFLDKATYLLVDDPKRIAW 150

Query: 146 RYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEKDRHFNY 205
           RY  TWL+ DV+S +P EL  S+L  + Q YG F+MLRLWRL RVS  FAR+EKDR +NY
Sbjct: 151 RYTSTWLIFDVVSTVPYELFGSLLHNTIQGYGIFSMLRLWRLHRVSKCFARLEKDRKYNY 210

Query: 206 FWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARYVTAIYW 265
           FW+RC KL+ V LF VH   C  Y I + + DP   ++ L   ++ ++SL  RYVTA+YW
Sbjct: 211 FWIRCTKLLLVSLFVVHCGACFCYSIAAHYPDPSMTFMALAEANWKQKSLLIRYVTAMYW 270

Query: 266 SIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTRKFRDTI 325
           SI T +TTGYGD+H  N  E  FI+ YM F LGL AY+IGNMTNLVVH TSRTR FRDTI
Sbjct: 271 SITTFSTTGYGDIHGNNAEERAFILFYMIFNLGLLAYIIGNMTNLVVHVTSRTRNFRDTI 330

Query: 326 QAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAHHLFYSL 385
           QAAS FAQRN LP+ LQEQM+AHL L++RTD EGLQQQE +DSLPKAIRSSI+H+LFY +
Sbjct: 331 QAASAFAQRNNLPLGLQEQMVAHLSLRYRTDSEGLQQQEIIDSLPKAIRSSISHYLFYEV 390

Query: 386 VDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLIIQRNGI 445
           VD  YLF+G+S DL+FQLV+EMKAEYFPPKED+IL+NEAPSDFYI+VTG  D+I + NG+
Sbjct: 391 VDKTYLFHGISNDLLFQLVSEMKAEYFPPKEDVILRNEAPSDFYIMVTGAVDIIARVNGV 450

Query: 446 EEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGDGTIIMD 505
           ++++GEA+TG V GE+GVLCY+PQLFTVRT RLSQLLRLNR++F NLVQA+VGDG IIM+
Sbjct: 451 DQVVGEAQTGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTAFLNLVQANVGDGAIIMN 510

Query: 506 NLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKRGSQPNE 565
           NLL+HLK+  DP+M+ I+ ET   L Q  M++PL+L +AA  GDDLLLHQLLKRGS PNE
Sbjct: 511 NLLQHLKDSTDPVMKGILAETELMLAQGKMDLPLSLCFAAARGDDLLLHQLLKRGSNPNE 570

Query: 566 VDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESIVKLLMD 625
            D  +G+TALH+AA++G+++CVVLLLE+GADPN RD EG+VPLW+AI G+HE   KLL +
Sbjct: 571 TD-KNGRTALHIAASKGSQYCVVLLLEHGADPNIRDSEGSVPLWEAIIGRHEENAKLLSE 630

Query: 626 NRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEGNSEMVK 685
           N A +S   V  F+C A  QN+++ L+ IV+ GGD++LS  NGTTALH AV EGN E+V+
Sbjct: 631 NGATLSFDTVGYFSCLAVGQNNLNALKDIVKYGGDISLSDVNGTTALHRAVSEGNLEIVQ 690

Query: 686 FLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSSVVHIPQIPEPNYLR 745
           FLLE+GAD+DKPDV+GWT RALA+HQGHE+IK LF  ++ + +    + +   PE   L 
Sbjct: 691 FLLEKGADMDKPDVYGWTARALAEHQGHEDIKALFYNQRPVER--KTILVSGTPEIKPLM 750

Query: 746 KFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFMSTINNGERDVLQPT 805
           K  SE  M       +M P+  +       +RR+ +N++NSL G MS    G+       
Sbjct: 751 KHSSEPVMTHHHSREAMPPLARA-----VSQRRKLSNFKNSLFGIMSAAKTGDEG---GA 810

Query: 806 SINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFGISVTKILTQ 865
           S     S+GV   Y ARVT+S   +   +GK+V LP S++EL+EIG KK G   TKIL++
Sbjct: 811 STRTGISEGVGGVYPARVTIS--GEASSSGKVVKLPDSLEELIEIGEKKLGFVATKILSR 870

Query: 866 EGAEVEDVNLLRDGDRLVLV 882
           EGAE++D+ ++RDGD L+L+
Sbjct: 871 EGAEIDDIRIIRDGDFLLLL 877

BLAST of Lcy01g017010 vs. ExPASy Swiss-Prot
Match: P0C550 (Potassium channel AKT1 OS=Oryza sativa subsp. indica OX=39946 GN=AKT1 PE=2 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 4.9e-285
Identity = 505/871 (57.98%), Postives = 646/871 (74.17%), Query Frame = 0

Query: 27  QLSRDGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETFLIVLVLYTAWV 86
           +LSRDGS YS++  ILP LG   R NRR+KLR  IISP+D  YR+WETFLIVLV+Y+AWV
Sbjct: 61  ELSRDGSHYSISSAILPSLG--ARSNRRIKLRRFIISPYDRRYRIWETFLIVLVVYSAWV 120

Query: 87  CPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEPKLIAMRYAKT 146
            PFEFGF+    G LA  DNVVN  FA+DI+LTFFVAYLDK +Y+L D+PK IA RY+ T
Sbjct: 121 SPFEFGFIPKPTGALATADNVVNAFFAVDIILTFFVAYLDKMSYMLEDDPKKIAWRYSTT 180

Query: 147 WLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEKDRHFNYFWVRC 206
           WLVLDV S IPSE AR ILP   ++YG+FNMLRLWRLRRVSS+F+R+EKDRHFNYFWVRC
Sbjct: 181 WLVLDVASTIPSEFARRILPSKLRSYGFFNMLRLWRLRRVSSLFSRLEKDRHFNYFWVRC 240

Query: 207 AKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARYVTAIYWSIATL 266
           AKLI V LF VH A C +YL+   +  P   W+  +  D+++ SLW RYVT++YWSI TL
Sbjct: 241 AKLICVTLFAVHCAACFYYLLADRYPVPTSTWIGNYMADFHERSLWIRYVTSVYWSITTL 300

Query: 267 TTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTRKFRDTIQAASN 326
           TT GYGDLHA N REM F I YM F LGL AYLIGNMTNLVVHGTSRTR +RDTIQAA++
Sbjct: 301 TTVGYGDLHAENTREMIFNIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNYRDTIQAATS 360

Query: 327 FAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAHHLFYSLVDSAY 386
           F  RNQLP RLQ+QM++H+ LK+RTD EGLQQQE +DSLPKAI+SSI+ +LF+ LV + Y
Sbjct: 361 FGVRNQLPPRLQDQMISHISLKYRTDSEGLQQQEILDSLPKAIKSSISQYLFFHLVQNVY 420

Query: 387 LFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLIIQRNGIEEIIG 446
           LF GVS DLIFQLV+EMKAEYFPP+ED+ILQNEAP+DFYI+V+G+ +L+ Q+NG +++I 
Sbjct: 421 LFQGVSNDLIFQLVSEMKAEYFPPREDVILQNEAPTDFYILVSGSVELVEQQNGADQVIQ 480

Query: 447 EAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGDGTIIMDNLLKH 506
            A +G+V+GEIGVLCY+PQLFTVRT  L QLLRLNR++F ++VQ++VGDGTIIM+NL++ 
Sbjct: 481 VATSGEVVGEIGVLCYRPQLFTVRTRSLCQLLRLNRTAFLSIVQSNVGDGTIIMNNLIQF 540

Query: 507 LKEIKD-PIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKRGSQPNEVDGS 566
           LKE K+  +M  +V E    L +  +++P+ L +A   GDD LLHQLLKRG  PNE D +
Sbjct: 541 LKEQKENSVMAGVVKEIESMLARGNLDLPITLCFAVTRGDDFLLHQLLKRGMDPNESD-N 600

Query: 567 SGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESIVKLLMDNRAN 626
            G TALH+AA++GNE CV LLLE GADPN RD EG VPLW+A+  KH ++V+LL++  A+
Sbjct: 601 DGHTALHIAASKGNEQCVRLLLEYGADPNARDSEGKVPLWEALCEKHAAVVQLLVEGGAD 660

Query: 627 ISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEGNSEMVKFLLE 686
           +SSGD   +AC A E++  ++L  I+  GGDV  +R +GTTALH AVC+GN +M + LLE
Sbjct: 661 LSSGDTGLYACIAVEESDTELLNDIIHYGGDVNRARRDGTTALHRAVCDGNVQMAELLLE 720

Query: 687 QGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSSVVHIPQIPEPNYLRKFQS 746
            GADIDK D +GWTPRALA+ QGH++I+ LF  +++    S   H+P            +
Sbjct: 721 HGADIDKQDGNGWTPRALAEQQGHDDIQLLFRSRKAAT-ASGHHHVPSSTTTRVAPAAAA 780

Query: 747 ESNMPRFSQDYSMTPVRESNYSDMPPR------RRRSNNYQNSLVGFMSTINNGERDVLQ 806
            S + RF+ +  M  +   + +D+P R      RR+   +QNSL G +S+ +  +R+   
Sbjct: 781 ASLIGRFNSEPMMKNMIHED-ADLPSRVLPEKLRRKRVTFQNSLFGVISS-SQAQRETDH 840

Query: 807 PTSI-------NFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFG 866
           P S        + + S G + A   RVT+SCPEKG  AGKLVLLP+++  LLE+GAKKF 
Sbjct: 841 PLSRGGLAATGSPNPSSGSRNAV-IRVTISCPEKGNTAGKLVLLPQTLDMLLELGAKKFD 900

Query: 867 ISVTKILTQEGAEVEDVNLLRDGDRLVLVSD 884
            + TK+LT EGAEV++V L+RDGD LVLVSD
Sbjct: 901 FAPTKVLTVEGAEVDEVELIRDGDHLVLVSD 924

BLAST of Lcy01g017010 vs. ExPASy Swiss-Prot
Match: Q0JKV1 (Potassium channel AKT1 OS=Oryza sativa subsp. japonica OX=39947 GN=AKT1 PE=2 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 1.9e-284
Identity = 504/871 (57.86%), Postives = 646/871 (74.17%), Query Frame = 0

Query: 27  QLSRDGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETFLIVLVLYTAWV 86
           +LSRDGS YS++  ILP LG   R NRR+KLR  IISP+D  YR+WETFLIVLV+Y+AWV
Sbjct: 61  ELSRDGSHYSISSAILPSLG--ARSNRRIKLRRFIISPYDRRYRIWETFLIVLVVYSAWV 120

Query: 87  CPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEPKLIAMRYAKT 146
            PFEFGF+    G LA  DNVVN  FA+DI+LTFFVAYLDK +Y+L D+PK IA RY+ T
Sbjct: 121 SPFEFGFIPKPTGALATADNVVNAFFAVDIILTFFVAYLDKMSYMLEDDPKKIAWRYSTT 180

Query: 147 WLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEKDRHFNYFWVRC 206
           WLVLDV S IPSE AR ILP   ++YG+FNMLRLWRLRRVSS+F+R+EKDRHFNYFWVRC
Sbjct: 181 WLVLDVASTIPSEFARRILPSKLRSYGFFNMLRLWRLRRVSSLFSRLEKDRHFNYFWVRC 240

Query: 207 AKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARYVTAIYWSIATL 266
           AKLI V LF VH A C +YL+   +  P   W+  +  D+++ SLW RYVT++YWSI TL
Sbjct: 241 AKLICVTLFAVHCAACFYYLLADRYPVPTSTWIGNYMADFHERSLWIRYVTSVYWSITTL 300

Query: 267 TTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTRKFRDTIQAASN 326
           TT GYGDLHA N REM F I YM F LGL AYLIGNMTNLVVHGTSRTR +RDTIQAA++
Sbjct: 301 TTVGYGDLHAENTREMIFNIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNYRDTIQAATS 360

Query: 327 FAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAHHLFYSLVDSAY 386
           F  RNQLP RLQ+QM++H+ LK+RTD EGLQQQE +DSLPKAI+SSI+ +LF+ LV + Y
Sbjct: 361 FGVRNQLPPRLQDQMISHISLKYRTDSEGLQQQEILDSLPKAIKSSISQYLFFHLVQNVY 420

Query: 387 LFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLIIQRNGIEEIIG 446
           LF GVS DLIFQLV+EMKAEYFPP+ED+ILQNEAP+DFYI+V+G+ +L+ Q+NG +++I 
Sbjct: 421 LFQGVSNDLIFQLVSEMKAEYFPPREDVILQNEAPTDFYILVSGSVELVEQQNGADQVIQ 480

Query: 447 EAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGDGTIIMDNLLKH 506
            A +G+V+GEIGVLCY+PQLFTVRT  L QLLRLNR++F ++VQ++VGDGTIIM+NL++ 
Sbjct: 481 VATSGEVVGEIGVLCYRPQLFTVRTRSLCQLLRLNRTAFLSIVQSNVGDGTIIMNNLIQF 540

Query: 507 LKEIKD-PIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKRGSQPNEVDGS 566
           LKE K+  +M  +V E    L +  +++P+ L +A   GDD LLHQLLKRG  PNE D +
Sbjct: 541 LKEQKENSVMAGVVKEIESMLARGNLDLPITLCFAVTRGDDFLLHQLLKRGMDPNESD-N 600

Query: 567 SGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESIVKLLMDNRAN 626
            G TALH+AA++GNE CV LLLE GADPN RD EG VPLW+A+  KH ++V+LL++  A+
Sbjct: 601 DGHTALHIAASKGNEQCVRLLLEYGADPNARDSEGKVPLWEALCEKHAAVVQLLVEGGAD 660

Query: 627 ISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEGNSEMVKFLLE 686
           +SSGD   +AC A E++  ++L  I+  GGDV  +R +GTTALH AVC+GN +M + LLE
Sbjct: 661 LSSGDTGLYACIAVEESDTELLNDIIHYGGDVNRARRDGTTALHRAVCDGNVQMAELLLE 720

Query: 687 QGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSSVVHIPQIPEPNYLRKFQS 746
            GADIDK D +GWTPRALA+ QGH++I+ LF  +++    S   H+P            +
Sbjct: 721 HGADIDKQDGNGWTPRALAEQQGHDDIQLLFRSRKAAT-ASGHHHVPSSTTTRVAPAAAA 780

Query: 747 ESNMPRFSQDYSMTPVRESNYSDMPPR------RRRSNNYQNSLVGFMSTINNGERDVLQ 806
            S + RF+ +  M  +   + +D+P R      RR+   +QNSL G +S+ +  +R+   
Sbjct: 781 ASLIGRFNSEPMMKNMIHED-ADLPSRVLPEKLRRKRVTFQNSLFGVISS-SQAQRETDH 840

Query: 807 PTSI-------NFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFG 866
           P S        + + S G + A   RVT+SCPEKG  AGKLVLLP+++  LLE+GAKKF 
Sbjct: 841 PLSRGGLAATGSPNPSSGSRNAV-IRVTISCPEKGNTAGKLVLLPQTLDMLLELGAKKFD 900

Query: 867 ISVTKILTQEGAEVEDVNLLRDGDRLVLVSD 884
            + TK+LT EGAEV++V L+RDGD LVLVS+
Sbjct: 901 FAPTKVLTVEGAEVDEVELIRDGDHLVLVSN 924

BLAST of Lcy01g017010 vs. ExPASy TrEMBL
Match: A0A6J1FDK8 (potassium channel AKT1-like OS=Cucurbita moschata OX=3662 GN=LOC111443129 PE=3 SV=1)

HSP 1 Score: 1490.7 bits (3858), Expect = 0.0e+00
Identity = 736/883 (83.35%), Postives = 809/883 (91.62%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG SMCGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPFDY
Sbjct: 1   MGLLGRSMCGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFDY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL +  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPSPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPPS Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPSLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIASNYKDPKRTWSTLYMEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            ESLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NESLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDTIQAASNFAQRNQLP RLQEQMLAHLCLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTIQAASNFAQRNQLPGRLQEQMLAHLCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+G HE IVKLL+DN A+ISSGDVAQFAC AAEQN+IDML+SI++CGGDVTL RSNGTTA
Sbjct: 601 IQGNHEPIVKLLIDNGADISSGDVAQFACTAAEQNNIDMLKSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH AVCEGNSEMVKFLLE GADIDK DVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHTAVCEGNSEMVKFLLEHGADIDKADVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NYLRKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYLRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ V+ AYQARV LSCPEKG+V GKLVLLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSVRSAYQARVVLSCPEKGQVIGKLVLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFGIS T ILT EGAEVED++L+RDGD L+LV DA AA+PS
Sbjct: 841 SKKFGISATMILTHEGAEVEDIDLVRDGDHLILVGDASAAVPS 882

BLAST of Lcy01g017010 vs. ExPASy TrEMBL
Match: A0A6J1IG00 (potassium channel AKT1-like OS=Cucurbita maxima OX=3661 GN=LOC111476603 PE=3 SV=1)

HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 729/883 (82.56%), Postives = 810/883 (91.73%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG S+CGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPF+Y
Sbjct: 1   MGLLGRSICGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFNY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL T  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPTPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPP+ Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPTLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIDSNYKDPKRTWSTLYMEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            ESLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NESLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDTIQAASNFAQRNQLP RLQEQMLAH+CLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTIQAASNFAQRNQLPGRLQEQMLAHMCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLF+GVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFSGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+  HE IVKLL+DN A+ISSGDVAQFAC AAEQN+IDML+SI++CGGDVTL RSNGTTA
Sbjct: 601 IQRNHEPIVKLLIDNGADISSGDVAQFACTAAEQNNIDMLKSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH+AVCEGNSE VKFLLE GADIDKPDVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHMAVCEGNSETVKFLLEHGADIDKPDVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NY+RKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYIRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ ++ AYQARV LSCPEKG+V GKL+LLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSMRSAYQARVVLSCPEKGQVIGKLMLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFGIS T ILT EGAEVED++L+RDGD L+LVSDA AA+PS
Sbjct: 841 SKKFGISATMILTHEGAEVEDIDLVRDGDHLILVSDASAAVPS 882

BLAST of Lcy01g017010 vs. ExPASy TrEMBL
Match: A0A6J1BTX7 (potassium channel AKT1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005323 PE=3 SV=1)

HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 712/894 (79.64%), Postives = 792/894 (88.59%), Query Frame = 0

Query: 2   SKQKSKKMGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPH 61
           S  K KKMGLLG SMCGR+ E+EIEQLSR DGSQYSLTGGILPPLGVNGR NRR KLR H
Sbjct: 3   SSHKKKKMGLLGRSMCGREPEKEIEQLSRDDGSQYSLTGGILPPLGVNGRNNRRGKLRAH 62

Query: 62  IISPFDYHYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTF 121
           IISPFDY+YRLWETFLI LVLYTAWVCPFEFGFL    GVLA+ DNVVNG FAIDI+LTF
Sbjct: 63  IISPFDYNYRLWETFLIFLVLYTAWVCPFEFGFLDMAKGVLAITDNVVNGFFAIDIILTF 122

Query: 122 FVAYLDKSTYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRL 181
           FVAYLDKS+YLLVD+PKLIA+RY KTWLV DV+S IPSELAR ILP   Q YGYFNMLRL
Sbjct: 123 FVAYLDKSSYLLVDDPKLIALRYVKTWLVFDVVSTIPSELARKILPLPVQTYGYFNMLRL 182

Query: 182 WRLRRVSSMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLT 241
           WRLRRVSSMFAR+EKDR+FNYFWVRCAKLIFVCLF VHFAGCCF+LI S   DPKK W+ 
Sbjct: 183 WRLRRVSSMFARLEKDRNFNYFWVRCAKLIFVCLFAVHFAGCCFFLIASHMSDPKKTWMG 242

Query: 242 LFYEDYNKESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLI 301
           LF +D+ +ESLW  YVT+IYWSI TLTTTGYGDLH VN++EM+F+ICY+FF+LGLQAYLI
Sbjct: 243 LFVDDFRQESLWYCYVTSIYWSIVTLTTTGYGDLHPVNEKEMSFVICYLFFVLGLQAYLI 302

Query: 302 GNMTNLVVHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQE 361
           GNMTNLVVHGT RTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLK RTD EGLQQQE
Sbjct: 303 GNMTNLVVHGTGRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKHRTDSEGLQQQE 362

Query: 362 TVDSLPKAIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 421
           TVD+LPKAIRSSI+HHLFYSLVDS YLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA
Sbjct: 363 TVDALPKAIRSSISHHLFYSLVDSVYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 422

Query: 422 PSDFYIVVTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL 481
           P+DFYIVVTG ADLIIQRNG+EEI+GEAK GDVIGEIGVLCY+PQLFTVRT RLSQLLR+
Sbjct: 423 PTDFYIVVTGAADLIIQRNGLEEIVGEAKPGDVIGEIGVLCYRPQLFTVRTCRLSQLLRM 482

Query: 482 NRSSFFNLVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYA 541
           NR+SFFNLVQASVGDG+IIM+NLLKHL+EIKDP+MEEI+ ET Q+LT+SGMEMP +L YA
Sbjct: 483 NRTSFFNLVQASVGDGSIIMNNLLKHLREIKDPMMEEILQETQQTLTRSGMEMPHSLCYA 542

Query: 542 AESGDDLLLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEG 601
           A +GDDLLLH+LLKRGS PNEVD S+GKT LH+AA++GNEHCVVLLLE GADPN+RDFEG
Sbjct: 543 ANNGDDLLLHKLLKRGSHPNEVDNSNGKTPLHIAASKGNEHCVVLLLEYGADPNQRDFEG 602

Query: 602 NVPLWQAIRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLS 661
           + PLW+AI+GKHESIVKLLMDN A+IS+GD+  FAC+AAEQNS DML+S+V CGGD+T  
Sbjct: 603 STPLWEAIQGKHESIVKLLMDNGADISAGDIGSFACSAAEQNSTDMLKSLVLCGGDITQP 662

Query: 662 RSNGTTALHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSG-K 721
           RSNGTTALH++VCEGNSEMVK LLEQG DIDKPD+HGWTPRALAD QGHEEIKELFS  K
Sbjct: 663 RSNGTTALHMSVCEGNSEMVKLLLEQGGDIDKPDIHGWTPRALADQQGHEEIKELFSAVK 722

Query: 722 QSMNKPSSVVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNY 781
           Q+ +KP + V IPQ  +  Y++KFQSESNMPRFSQ+ SMTPVRESN+SD+PPRRRR+NNY
Sbjct: 723 QAASKPHT-VQIPQSLDLPYMQKFQSESNMPRFSQESSMTPVRESNFSDIPPRRRRNNNY 782

Query: 782 QNSLVGFMSTINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRS 841
           +NSLVGFM+T N G+ D     S  +S  Q V   Y+ARVT+SCPEKG  A KLVLLP S
Sbjct: 783 RNSLVGFMATANTGDGDSQPSASHGYSGGQSVGEGYRARVTISCPEKGN-AAKLVLLPNS 842

Query: 842 IQELLEIGAKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPSSHQ 894
           IQELLEIGAKKFG   TKILTQEGAE+ED++L+RDGDRL L  DAGA +PSSHQ
Sbjct: 843 IQELLEIGAKKFGFYATKILTQEGAEIEDIDLIRDGDRLFLAGDAGATLPSSHQ 894

BLAST of Lcy01g017010 vs. ExPASy TrEMBL
Match: A0A6J1BU03 (potassium channel AKT1 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005323 PE=3 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 710/894 (79.42%), Postives = 789/894 (88.26%), Query Frame = 0

Query: 2   SKQKSKKMGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPH 61
           S  K KKMGLLG SMCGR+ E+EIEQLSR DGSQYSLTGGILPPLGVNGR NRR KLR H
Sbjct: 3   SSHKKKKMGLLGRSMCGREPEKEIEQLSRDDGSQYSLTGGILPPLGVNGRNNRRGKLRAH 62

Query: 62  IISPFDYHYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTF 121
           IISPFDY+YRLWETFLI LVLYTAWVCPFEFGFL    GVLA+ DNVVNG FAIDI+LTF
Sbjct: 63  IISPFDYNYRLWETFLIFLVLYTAWVCPFEFGFLDMAKGVLAITDNVVNGFFAIDIILTF 122

Query: 122 FVAYLDKSTYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRL 181
           FVAYLDKS+YLLVD+PKLIA+RY KTWLV DV+S IPSELAR ILP   Q YGYFNMLRL
Sbjct: 123 FVAYLDKSSYLLVDDPKLIALRYVKTWLVFDVVSTIPSELARKILPLPVQTYGYFNMLRL 182

Query: 182 WRLRRVSSMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLT 241
           WRLRRVSSMFAR+EKDR+FNYFWVRCAKLIFVCLF VHFAGCCF+LI S   DPKK W+ 
Sbjct: 183 WRLRRVSSMFARLEKDRNFNYFWVRCAKLIFVCLFAVHFAGCCFFLIASHMSDPKKTWMG 242

Query: 242 LFYEDYNKESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLI 301
           LF +D+ +ESLW  YVT+IYWSI TLTTTGYGDLH VN++EM+F+ICY+FF+LGLQAYLI
Sbjct: 243 LFVDDFRQESLWYCYVTSIYWSIVTLTTTGYGDLHPVNEKEMSFVICYLFFVLGLQAYLI 302

Query: 302 GNMTNLVVHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQE 361
           GNMTNLVVHGT RTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLK RTD EGLQQQE
Sbjct: 303 GNMTNLVVHGTGRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKHRTDSEGLQQQE 362

Query: 362 TVDSLPKAIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 421
           TVD+LPKAIRSSI+HHLFYSLVDS YLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA
Sbjct: 363 TVDALPKAIRSSISHHLFYSLVDSVYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 422

Query: 422 PSDFYIVVTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL 481
           P+DFYIVVTG ADLIIQRNG+EEI+GEAK GDVIGEIGVLCY+PQLFTVRT RLSQLLR+
Sbjct: 423 PTDFYIVVTGAADLIIQRNGLEEIVGEAKPGDVIGEIGVLCYRPQLFTVRTCRLSQLLRM 482

Query: 482 NRSSFFNLVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYA 541
           NR+SFFNLVQASVGDG+IIM+NLLKHL+EIKDP+MEEI+ ET Q+LT+SGMEMP +L YA
Sbjct: 483 NRTSFFNLVQASVGDGSIIMNNLLKHLREIKDPMMEEILQETQQTLTRSGMEMPHSLCYA 542

Query: 542 AESGDDLLLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEG 601
           A +GDDLLLH+LLKRGS PNEVD S+GKT LH+AA++GNEHCVVLLLE GADPN+RDFEG
Sbjct: 543 ANNGDDLLLHKLLKRGSHPNEVDNSNGKTPLHIAASKGNEHCVVLLLEYGADPNQRDFEG 602

Query: 602 NVPLWQAIRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLS 661
           + PLW+AI+GKHESIVKLLMDN A+IS+GD+  FAC+AAEQNS DML+S+V CGGD+T  
Sbjct: 603 STPLWEAIQGKHESIVKLLMDNGADISAGDIGSFACSAAEQNSTDMLKSLVLCGGDITQP 662

Query: 662 RSNGTTALHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSG-K 721
           RSNGTTALH++VCEGNSEMVK LLEQG DIDKPD+HGWTPRALAD QGHEEIKELFS  K
Sbjct: 663 RSNGTTALHMSVCEGNSEMVKLLLEQGGDIDKPDIHGWTPRALADQQGHEEIKELFSAVK 722

Query: 722 QSMNKPSSVVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNY 781
           Q+ +KP + V IPQ  +  Y++KFQSESNMPRFSQ+ SMTPVRESN+SD+PPRRRR+NNY
Sbjct: 723 QAASKPHT-VQIPQSLDLPYMQKFQSESNMPRFSQESSMTPVRESNFSDIPPRRRRNNNY 782

Query: 782 QNSLVGFMSTINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRS 841
           +NSLVGFM+T N GE          F  +  V   Y+ARVT+SCPEKG  A KLVLLP S
Sbjct: 783 RNSLVGFMATANTGE-----IAFCIFEQTISVGEGYRARVTISCPEKGN-AAKLVLLPNS 842

Query: 842 IQELLEIGAKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPSSHQ 894
           IQELLEIGAKKFG   TKILTQEGAE+ED++L+RDGDRL L  DAGA +PSSHQ
Sbjct: 843 IQELLEIGAKKFGFYATKILTQEGAEIEDIDLIRDGDRLFLAGDAGATLPSSHQ 889

BLAST of Lcy01g017010 vs. ExPASy TrEMBL
Match: A0A6J1BT75 (potassium channel AKT1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005323 PE=3 SV=1)

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 711/911 (78.05%), Postives = 794/911 (87.16%), Query Frame = 0

Query: 2   SKQKSKKMGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPH 61
           S  K KKMGLLG SMCGR+ E+EIEQLSR DGSQYSLTGGILPPLGVNGR NRR KLR H
Sbjct: 3   SSHKKKKMGLLGRSMCGREPEKEIEQLSRDDGSQYSLTGGILPPLGVNGRNNRRGKLRAH 62

Query: 62  IISPFDYHYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTF 121
           IISPFDY+YRLWETFLI LVLYTAWVCPFEFGFL    GVLA+ DNVVNG FAIDI+LTF
Sbjct: 63  IISPFDYNYRLWETFLIFLVLYTAWVCPFEFGFLDMAKGVLAITDNVVNGFFAIDIILTF 122

Query: 122 FVAYLDKSTYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRL 181
           FVAYLDKS+YLLVD+PKLIA+RY KTWLV DV+S IPSELAR ILP   Q YGYFNMLRL
Sbjct: 123 FVAYLDKSSYLLVDDPKLIALRYVKTWLVFDVVSTIPSELARKILPLPVQTYGYFNMLRL 182

Query: 182 WRLRRVSSMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLT 241
           WRLRRVSSMFAR+EKDR+FNYFWVRCAKLIFVCLF VHFAGCCF+LI S   DPKK W+ 
Sbjct: 183 WRLRRVSSMFARLEKDRNFNYFWVRCAKLIFVCLFAVHFAGCCFFLIASHMSDPKKTWMG 242

Query: 242 LFYEDYNKESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLI 301
           LF +D+ +ESLW  YVT+IYWSI TLTTTGYGDLH VN++EM+F+ICY+FF+LGLQAYLI
Sbjct: 243 LFVDDFRQESLWYCYVTSIYWSIVTLTTTGYGDLHPVNEKEMSFVICYLFFVLGLQAYLI 302

Query: 302 GNMTNLVVHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQE 361
           GNMTNLVVHGT RTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLK RTD EGLQQQE
Sbjct: 303 GNMTNLVVHGTGRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKHRTDSEGLQQQE 362

Query: 362 TVDSLPKAIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 421
           TVD+LPKAIRSSI+HHLFYSLVDS YLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA
Sbjct: 363 TVDALPKAIRSSISHHLFYSLVDSVYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEA 422

Query: 422 PSDFYIVVTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL 481
           P+DFYIVVTG ADLIIQRNG+EEI+GEAK GDVIGEIGVLCY+PQLFTVRT RLSQLLR+
Sbjct: 423 PTDFYIVVTGAADLIIQRNGLEEIVGEAKPGDVIGEIGVLCYRPQLFTVRTCRLSQLLRM 482

Query: 482 NRSSFFNLVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYA 541
           NR+SFFNLVQASVGDG+IIM+NLLKHL+EIKDP+MEEI+ ET Q+LT+SGMEMP +L YA
Sbjct: 483 NRTSFFNLVQASVGDGSIIMNNLLKHLREIKDPMMEEILQETQQTLTRSGMEMPHSLCYA 542

Query: 542 AESGDDLLLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEG 601
           A +GDDLLLH+LLKRGS PNEVD S+GKT LH+AA++GNEHCVVLLLE GADPN+RDFEG
Sbjct: 543 ANNGDDLLLHKLLKRGSHPNEVDNSNGKTPLHIAASKGNEHCVVLLLEYGADPNQRDFEG 602

Query: 602 NVPLWQAIRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLS 661
           + PLW+AI+GKHESIVKLLMDN A+IS+GD+  FAC+AAEQNS DML+S+V CGGD+T  
Sbjct: 603 STPLWEAIQGKHESIVKLLMDNGADISAGDIGSFACSAAEQNSTDMLKSLVLCGGDITQP 662

Query: 662 RSNGTTALHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSG-K 721
           RSNGTTALH++VCEGNSEMVK LLEQG DIDKPD+HGWTPRALAD QGHEEIKELFS  K
Sbjct: 663 RSNGTTALHMSVCEGNSEMVKLLLEQGGDIDKPDIHGWTPRALADQQGHEEIKELFSAVK 722

Query: 722 QSMNKPSSVVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNY 781
           Q+ +KP + V IPQ  +  Y++KFQSESNMPRFSQ+ SMTPVRESN+SD+PPRRRR+NNY
Sbjct: 723 QAASKPHT-VQIPQSLDLPYMQKFQSESNMPRFSQESSMTPVRESNFSDIPPRRRRNNNY 782

Query: 782 QNSLVGFMSTINNGE--------RDVLQPTSIN---------FSNSQGVKVAYQARVTLS 841
           +NSLVGFM+T N GE          VL P+++N          + ++   V    RVT+S
Sbjct: 783 RNSLVGFMATANTGEIAFCIFEQTIVLIPSALNQRCPLRKLIRNQNKLQSVLLSTRVTIS 842

Query: 842 CPEKGEVAGKLVLLPRSIQELLEIGAKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVS 894
           CPEKG  A KLVLLP SIQELLEIGAKKFG   TKILTQEGAE+ED++L+RDGDRL L  
Sbjct: 843 CPEKGN-AAKLVLLPNSIQELLEIGAKKFGFYATKILTQEGAEIEDIDLIRDGDRLFLAG 902

BLAST of Lcy01g017010 vs. NCBI nr
Match: XP_022936563.1 (potassium channel AKT1-like [Cucurbita moschata])

HSP 1 Score: 1490.7 bits (3858), Expect = 0.0e+00
Identity = 736/883 (83.35%), Postives = 809/883 (91.62%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG SMCGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPFDY
Sbjct: 1   MGLLGRSMCGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFDY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL +  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPSPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPPS Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPSLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIASNYKDPKRTWSTLYMEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            ESLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NESLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDTIQAASNFAQRNQLP RLQEQMLAHLCLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTIQAASNFAQRNQLPGRLQEQMLAHLCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+G HE IVKLL+DN A+ISSGDVAQFAC AAEQN+IDML+SI++CGGDVTL RSNGTTA
Sbjct: 601 IQGNHEPIVKLLIDNGADISSGDVAQFACTAAEQNNIDMLKSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH AVCEGNSEMVKFLLE GADIDK DVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHTAVCEGNSEMVKFLLEHGADIDKADVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NYLRKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYLRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ V+ AYQARV LSCPEKG+V GKLVLLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSVRSAYQARVVLSCPEKGQVIGKLVLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFGIS T ILT EGAEVED++L+RDGD L+LV DA AA+PS
Sbjct: 841 SKKFGISATMILTHEGAEVEDIDLVRDGDHLILVGDASAAVPS 882

BLAST of Lcy01g017010 vs. NCBI nr
Match: KAG7024473.1 (Potassium channel AKT1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 734/883 (83.13%), Postives = 806/883 (91.28%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG SMCGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPFDY
Sbjct: 1   MGLLGRSMCGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFDY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL +  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPSPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPPS Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPSLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIDSNYKDPKRTWSTLYMEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            ESLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NESLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDTIQAASNFAQRNQLP RLQEQMLAHLCLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTIQAASNFAQRNQLPGRLQEQMLAHLCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+G HE IVKLL+DN A+ISSGDVAQFAC A EQN+IDML SI++CGGDVTL RSNGTTA
Sbjct: 601 IQGNHEPIVKLLIDNGADISSGDVAQFACTAVEQNNIDMLTSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH AVCEGNSEMVKFLLE GADIDK DVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHTAVCEGNSEMVKFLLEHGADIDKADVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NYLRKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYLRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ V+ AYQARV LSCPEKG+V GKLVLLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSVRSAYQARVVLSCPEKGQVIGKLVLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFGIS T ILT EGAEVED++L+RDGD L+LV DA A +PS
Sbjct: 841 SKKFGISATMILTHEGAEVEDIDLVRDGDHLILVGDASATVPS 882

BLAST of Lcy01g017010 vs. NCBI nr
Match: XP_023535584.1 (potassium channel AKT1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1484.5 bits (3842), Expect = 0.0e+00
Identity = 731/883 (82.79%), Postives = 808/883 (91.51%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG SMCGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPFDY
Sbjct: 1   MGLLGRSMCGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFDY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL +  GVLA+IDNVVNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPSPSGVLAIIDNVVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPPS Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPSLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIDSNYKDPKRTWSTLYIEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            +SLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NKSLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDT+QAASNFAQRNQLP RLQEQMLAHLCLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTVQAASNFAQRNQLPGRLQEQMLAHLCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNG+EEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGMEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  +GKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGNGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+G HE IVKLL+DN A+ISSGDVAQFAC A EQN+IDML+SI++CGGDVTL RSNGTTA
Sbjct: 601 IQGNHEPIVKLLIDNGADISSGDVAQFACTAVEQNNIDMLKSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH AVCEGNSEMVKFLLE GADIDK DVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHTAVCEGNSEMVKFLLEHGADIDKADVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NYLRKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYLRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ V+ AYQARV LSCPEKG+V GKLVLLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSVRSAYQARVVLSCPEKGQVIGKLVLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFG+S T ILT EGAEVED++L+RDGD L+LV DA AA+PS
Sbjct: 841 SKKFGVSATMILTHEGAEVEDIDLVRDGDHLILVGDASAAVPS 882

BLAST of Lcy01g017010 vs. NCBI nr
Match: XP_022976101.1 (potassium channel AKT1-like [Cucurbita maxima])

HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 729/883 (82.56%), Postives = 810/883 (91.73%), Query Frame = 0

Query: 9   MGLLGISMCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDY 68
           MGLLG S+CGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPF+Y
Sbjct: 1   MGLLGRSICGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFNY 60

Query: 69  HYRLWETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDK 128
           +YR WETFLI LVLYTAW CPFEFGFL T  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK
Sbjct: 61  NYRFWETFLIFLVLYTAWACPFEFGFLPTPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDK 120

Query: 129 STYLLVDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVS 188
           +TYLLVD+ KLIA+RYAKTWLVLDV+S IPSE+AR+ILPP+ Q YGYFNMLRLWRLRRVS
Sbjct: 121 TTYLLVDDRKLIALRYAKTWLVLDVVSNIPSEMARNILPPTLQVYGYFNMLRLWRLRRVS 180

Query: 189 SMFARMEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYN 248
           SMFAR+EKDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++
Sbjct: 181 SMFARLEKDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIDSNYKDPKRTWSTLYMEDFH 240

Query: 249 KESLWARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLV 308
            ESLW RYVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLV
Sbjct: 241 NESLWVRYVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLV 300

Query: 309 VHGTSRTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPK 368
           VHGTSRTR+FRDTIQAASNFAQRNQLP RLQEQMLAH+CLKFR D EGLQQQET+DSLPK
Sbjct: 301 VHGTSRTRQFRDTIQAASNFAQRNQLPGRLQEQMLAHMCLKFRIDSEGLQQQETIDSLPK 360

Query: 369 AIRSSIAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIV 428
           AIRS IAHHLFYSLVDSAYLF+GVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIV
Sbjct: 361 AIRSGIAHHLFYSLVDSAYLFSGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIV 420

Query: 429 VTGTADLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 488
           VTGTADLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN
Sbjct: 421 VTGTADLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFN 480

Query: 489 LVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDL 548
           LVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++AA SGDDL
Sbjct: 481 LVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFAASSGDDL 540

Query: 549 LLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQA 608
           LLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG++PLWQA
Sbjct: 541 LLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEGSLPLWQA 600

Query: 609 IRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTA 668
           I+  HE IVKLL+DN A+ISSGDVAQFAC AAEQN+IDML+SI++CGGDVTL RSNGTTA
Sbjct: 601 IQRNHEPIVKLLIDNGADISSGDVAQFACTAAEQNNIDMLKSIIECGGDVTLPRSNGTTA 660

Query: 669 LHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSS 728
           LH+AVCEGNSE VKFLLE GADIDKPDVHGWTPRALADHQGHEEIKELFS KQ++NK SS
Sbjct: 661 LHMAVCEGNSETVKFLLEHGADIDKPDVHGWTPRALADHQGHEEIKELFSLKQAVNK-SS 720

Query: 729 VVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM 788
           VVHIPQ PE NY+RKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+NSLVGFM
Sbjct: 721 VVHIPQNPESNYIRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYRNSLVGFM 780

Query: 789 STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIG 848
              N GERDVLQ   + FSNS+ ++ AYQARV LSCPEKG+V GKL+LLP++IQEL+E G
Sbjct: 781 ENTNKGERDVLQSALLGFSNSRSMRSAYQARVVLSCPEKGQVIGKLMLLPKTIQELVETG 840

Query: 849 AKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           +KKFGIS T ILT EGAEVED++L+RDGD L+LVSDA AA+PS
Sbjct: 841 SKKFGISATMILTHEGAEVEDIDLVRDGDHLILVSDASAAVPS 882

BLAST of Lcy01g017010 vs. NCBI nr
Match: KAG6591585.1 (Potassium channel AKT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1466.4 bits (3795), Expect = 0.0e+00
Identity = 728/890 (81.80%), Postives = 800/890 (89.89%), Query Frame = 0

Query: 16  MCGRDQEREIEQLSR-DGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWET 75
           MCGR+ +RE+E LSR DGSQYSLTGGILPPLGVNGR NRR KL PHIISPFDY+YR WET
Sbjct: 1   MCGREDQRELEHLSRDDGSQYSLTGGILPPLGVNGRNNRRSKLGPHIISPFDYNYRFWET 60

Query: 76  FLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVD 135
           FLI LVLYTAW CPFEFGFL +  GVLA+IDN+VNG+FAIDIVLTFFVAYLDK+TYLLVD
Sbjct: 61  FLIFLVLYTAWACPFEFGFLPSPSGVLAIIDNIVNGLFAIDIVLTFFVAYLDKTTYLLVD 120

Query: 136 EPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARME 195
           + KLIA+RYAKTWLVLDV+S IPSE+AR+ILPPS Q YGYFNMLRLWRLRRVSSMFAR+E
Sbjct: 121 DRKLIALRYAKTWLVLDVVSNIPSEMARNILPPSLQVYGYFNMLRLWRLRRVSSMFARLE 180

Query: 196 KDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWAR 255
           KDR++NYFWVR AKL+FVCLFTVHFAGCCFYLI S++KDPK+ W TL+ ED++ ESLW R
Sbjct: 181 KDRNYNYFWVRSAKLMFVCLFTVHFAGCCFYLIDSNYKDPKRTWSTLYMEDFHNESLWVR 240

Query: 256 YVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRT 315
           YVT+IYWSI T+TTTGYGDLH +ND+EM+F+ICY+FFILGLQ+YLIGNMTNLVVHGTSRT
Sbjct: 241 YVTSIYWSIVTITTTGYGDLHPINDQEMSFVICYLFFILGLQSYLIGNMTNLVVHGTSRT 300

Query: 316 RKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIA 375
           R+FRDTIQAASNFAQRNQLP RLQEQMLAHLCLKFR D EGLQQQET+DSLPKAIRS IA
Sbjct: 301 RQFRDTIQAASNFAQRNQLPGRLQEQMLAHLCLKFRIDSEGLQQQETIDSLPKAIRSGIA 360

Query: 376 HHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTA-- 435
           HHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAP+DFYIVVTGTA  
Sbjct: 361 HHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPTDFYIVVTGTALK 420

Query: 436 ------------DLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL 495
                       DLI+QRNGIEEI+GEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL
Sbjct: 421 QTISSDVCFTLQDLIVQRNGIEEIVGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRL 480

Query: 496 NRSSFFNLVQASVGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYA 555
           NRSSFFNLVQASVGDGTIIM+NLLKHLKE+KDP+MEEI+ ET QSL ++GMEMPLNL++A
Sbjct: 481 NRSSFFNLVQASVGDGTIIMNNLLKHLKEVKDPMMEEILQETEQSLIRAGMEMPLNLYFA 540

Query: 556 AESGDDLLLHQLLKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEG 615
           A SGDDLLLHQLLKRGS PNEVD  SGKTALH+AAA+GNEHCVVLLLE GADPNRRDFEG
Sbjct: 541 ASSGDDLLLHQLLKRGSNPNEVDEGSGKTALHIAAAKGNEHCVVLLLEYGADPNRRDFEG 600

Query: 616 NVPLWQAIRGKHESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLS 675
           ++PLWQAI+G HE IVKLL+DN A+ISSGDVAQFAC A EQN+IDML SI++CGGDVTL 
Sbjct: 601 SLPLWQAIQGNHEPIVKLLIDNGADISSGDVAQFACTAVEQNNIDMLTSIIECGGDVTLP 660

Query: 676 RSNGTTALHLAVCEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQ 735
           RSNGTTALH AVCEGNSEMVKFLLE GADIDK DVHGWTPRALADHQGHEEIKELFS KQ
Sbjct: 661 RSNGTTALHTAVCEGNSEMVKFLLEHGADIDKADVHGWTPRALADHQGHEEIKELFSLKQ 720

Query: 736 SMNKPSSVVHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQ 795
           ++NK SSVVHIPQ PE NYLRKFQSES +PRFS D  MTPVRESN SDMPPRRRRSNNY+
Sbjct: 721 AVNK-SSVVHIPQNPESNYLRKFQSESIIPRFSHDSCMTPVRESNCSDMPPRRRRSNNYR 780

Query: 796 NSLVGFMSTINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSI 855
           NSLVGFM   N GERDVLQ   + FSNS+ V+ AYQARV LSCPEKG+V GKLVLLP++I
Sbjct: 781 NSLVGFMENTNKGERDVLQSALLGFSNSRSVRSAYQARVVLSCPEKGQVIGKLVLLPKTI 840

Query: 856 QELLEIGAKKFGISVTKILTQEGAEVEDVNLLRDGDRLVLVSDAGAAIPS 891
           QEL+E G+KKFGIS T ILT EGAEVED++L+RDGD L+LV DA A +PS
Sbjct: 841 QELVETGSKKFGISATMILTHEGAEVEDIDLVRDGDHLILVGDASATVPS 889

BLAST of Lcy01g017010 vs. TAIR 10
Match: AT2G25600.1 (Shaker pollen inward K+ channel )

HSP 1 Score: 1003.4 bits (2593), Expect = 1.1e-292
Identity = 513/870 (58.97%), Postives = 647/870 (74.37%), Query Frame = 0

Query: 19  RDQEREI-EQLSRDG--SQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETF 78
           +D E ++ + LSRDG  SQYSL+ G+LP LG N R +R V L   I+SPFD  YR WETF
Sbjct: 27  KDAEDDVADHLSRDGTMSQYSLSKGLLPSLGANNRSSRDVILPRFIVSPFDPRYRAWETF 86

Query: 79  LIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDE 138
           L+ LVLYTAW  PFEFGFL+     L+++DN+VNG FA+DIVLTFFVA+LDK TYLLVD+
Sbjct: 87  LVFLVLYTAWASPFEFGFLQKPRPPLSILDNIVNGFFAVDIVLTFFVAFLDKVTYLLVDD 146

Query: 139 PKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEK 198
           PK IA RYA TWL+ DV+S  P E+  S+L  S Q YG F+MLRLWRLRRVS+ FAR+EK
Sbjct: 147 PKRIAWRYASTWLIFDVVSTFPYEIFGSLLHESIQGYGIFSMLRLWRLRRVSNCFARLEK 206

Query: 199 DRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARY 258
           DR ++YFWVRC+KL+ V LF +H   C  Y I + + DP K ++ L  E++ +  +  RY
Sbjct: 207 DRKYSYFWVRCSKLLLVTLFVIHCGACFLYSIAAHYPDPSKTFMALTDENWKESPIAVRY 266

Query: 259 VTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTR 318
            TA+YWSI T +TTGYGD+H VN REMTFI+ YM F LGL AY+IGNMTNLVVH T RTR
Sbjct: 267 NTAMYWSITTFSTTGYGDIHGVNSREMTFILFYMVFNLGLSAYIIGNMTNLVVHVTGRTR 326

Query: 319 KFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAH 378
           KFRDTIQAAS F QRN LPVRLQ+QM+AHLCL++RTD EGLQQQE +DSLPKAIRSSI+H
Sbjct: 327 KFRDTIQAASGFGQRNNLPVRLQDQMVAHLCLRYRTDSEGLQQQEIIDSLPKAIRSSISH 386

Query: 379 HLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLI 438
           +LFY +VD  YLF+G+S DL+FQLVTEMKAEYFPPKED+ILQNEAP+DFYI+VTG  D+I
Sbjct: 387 YLFYEVVDKIYLFHGISNDLLFQLVTEMKAEYFPPKEDVILQNEAPTDFYILVTGAVDII 446

Query: 439 IQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGD 498
            + NG+E+++ EA+ G V GE+GVLCY+PQLFTVRT RLSQLLRLNR+   NLVQA+VGD
Sbjct: 447 ARVNGVEQVVSEAQRGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTVLLNLVQANVGD 506

Query: 499 GTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKR 558
           G IIM+NLL+HLK+ +DP+M+ ++ +T   L Q  M++PL+L +AA  GDDLLLHQLL+R
Sbjct: 507 GAIIMNNLLQHLKDSEDPVMKGVLADTEHMLAQGKMDLPLSLCFAAARGDDLLLHQLLRR 566

Query: 559 GSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESI 618
           GS PNE+D   G+TALH+AA++G+ +CVVLLLE+GADPN RD EGNVPLW+AI G+H  I
Sbjct: 567 GSSPNEMD-KDGRTALHIAASKGSHYCVVLLLEHGADPNIRDSEGNVPLWEAIIGRHREI 626

Query: 619 VKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEG 678
            KLL +N A +S   V+ F+  A E+N +D L+ I++ GGDVTL   NGTTALH AV EG
Sbjct: 627 AKLLAENGAKLSLDSVSYFSGLAVEKNCLDALKDIIKYGGDVTLPDGNGTTALHRAVSEG 686

Query: 679 NSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSM-NKPSSVVHIPQI 738
           + E+VKFLL+QGAD+D PD +GWTPR LADHQG+EEIK LF   + +  KP  +  IPQ 
Sbjct: 687 HLEIVKFLLDQGADLDWPDSYGWTPRGLADHQGNEEIKTLFHNHRPVEKKPKPIPGIPQS 746

Query: 739 PEPNY-LRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFMSTINNG 798
           P     L K+ SE  M      +S   V +     +  ++R+ NN++NSL G +S  N+ 
Sbjct: 747 PVTGKPLMKYSSEPTM------HSGELVLDGG-QVVVSQKRKLNNFRNSLFGIISAANSA 806

Query: 799 ERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFGI 858
           +     P S       G  + Y  RVT+S PE GE  GK+VLLP S++ELL+IG  K G 
Sbjct: 807 DDGGEVPRSPAVPGGGG-SMIYPERVTISSPENGETGGKVVLLPNSMEELLKIGENKMGF 866

Query: 859 SVTKILTQEGAEVEDVNLLRDGDRLVLVSD 884
             TK+LT+EGAE++D+ L+RDGD L+L  D
Sbjct: 867 VPTKVLTREGAEIDDITLIRDGDFLLLSRD 887

BLAST of Lcy01g017010 vs. TAIR 10
Match: AT2G26650.1 (K+ transporter 1 )

HSP 1 Score: 1000.7 bits (2586), Expect = 7.3e-292
Identity = 520/880 (59.09%), Postives = 658/880 (74.77%), Query Frame = 0

Query: 13  GISMCGRDQEREIEQLSRDGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLW 72
           G  +CG+ Q+ EIEQLSR+ S +SL+ GILP LG   R NRRVKLR  ++SP+D+ YR+W
Sbjct: 4   GALLCGQVQD-EIEQLSRESSHFSLSTGILPSLG--ARSNRRVKLRRFVVSPYDHKYRIW 63

Query: 73  ETFLIVLVLYTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLL 132
           E FL+VLV+YTAWV PFEFGFL+     L++ DN+VN  FAIDI++TFFV YLDKSTYL+
Sbjct: 64  EAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLI 123

Query: 133 VDEPKLIAMRYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFAR 192
           VD+ K IA +Y ++W +LD++S IPSE A  I   S+Q+YG FNMLRLWRLRRV ++FAR
Sbjct: 124 VDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRI---SSQSYGLFNMLRLWRLRRVGALFAR 183

Query: 193 MEKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLW 252
           +EKDR+FNYFWVRCAKL+ V LF VH A C +YLI +   +P K W+     ++ +ESLW
Sbjct: 184 LEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLW 243

Query: 253 ARYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTS 312
            RYVT++YWSI TLTT GYGDLH VN +EM F I YM F LGL AYLIGNMTNLVVHGTS
Sbjct: 244 MRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTS 303

Query: 313 RTRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSS 372
           RTR FRDTIQAASNFA RN LP RLQ+QMLAHLCLK+RTD EGLQQQET+D+LPKAIRSS
Sbjct: 304 RTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSS 363

Query: 373 IAHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTA 432
           I+H LFYSL+D  YLF GVS DL+FQLV+EMKAEYFPPKED+ILQNEAP+DFYI+V GTA
Sbjct: 364 ISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTA 423

Query: 433 DLIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQAS 492
           DL+    G E I+ E K GD+IGEIGVLCY+PQLFTVRT RL QLLR+NR++F N++QA+
Sbjct: 424 DLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQAN 483

Query: 493 VGDGTIIMDNLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQL 552
           VGDGTIIM+NLL+HLKE+ DP+M  ++ E    L +  M++PLNL +AA   DDLLLHQL
Sbjct: 484 VGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQL 543

Query: 553 LKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKH 612
           LKRG  PNE D ++G+T LH+AA++G  +CV+LLLE  ADPN RD EG+VPLW+A+   H
Sbjct: 544 LKRGLDPNESD-NNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGH 603

Query: 613 ESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAV 672
           E +VK+L+++ + I +GDV  FAC AAEQ ++ +L+ IV  GGDVT  R+ GT+ALH AV
Sbjct: 604 EKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAV 663

Query: 673 CEGNSEMVKFLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNK----PSSV 732
           CE N EMVK+LLEQGAD++K D+HGWTPR LA+ QGHE+IK LF  K    +     SS 
Sbjct: 664 CEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSS 723

Query: 733 VHIPQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFM- 792
           V I +     +L +F SE N+   S++ S   +RE+        RR++NN+ NSL G + 
Sbjct: 724 VPILK-TGIRFLGRFTSEPNIRPASREVSFR-IRETR------ARRKTNNFDNSLFGILA 783

Query: 793 --STINNGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLE 852
             S   NG   V +  + N             RVT+SC EK ++AGKLVLLP S +ELLE
Sbjct: 784 NQSVPKNGLATVDEGRTGN-----------PVRVTISCAEKDDIAGKLVLLPGSFKELLE 843

Query: 853 IGAKKFGISVTKILTQE-GAEVEDVNLLRDGDRLVLVSDA 885
           +G+ KFGI  TK++ ++  AE++DV+++RDGD L+  +D+
Sbjct: 844 LGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS 857

BLAST of Lcy01g017010 vs. TAIR 10
Match: AT4G32500.1 (K+ transporter 5 )

HSP 1 Score: 986.5 bits (2549), Expect = 1.4e-287
Identity = 502/860 (58.37%), Postives = 642/860 (74.65%), Query Frame = 0

Query: 26  EQLSRDG--SQYSLTGGILPPLGV--NGRKNRRVKLRPHIISPFDYHYRLWETFLIVLVL 85
           E +SR+G  S YS + G+LPPLGV    R +R +KLR  I+SPFD  YR W+ FL++LVL
Sbjct: 31  EHISREGTMSHYSFSKGLLPPLGVGATARSSRHIKLRCFIVSPFDPRYRAWDWFLVILVL 90

Query: 86  YTAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEPKLIAM 145
           YTAW  PFEFGFL+T    L+++DNVVNG FA+DIVLTFFVA+LDK+TYLLVD+PK IA 
Sbjct: 91  YTAWASPFEFGFLQTPRAPLSILDNVVNGFFAVDIVLTFFVAFLDKATYLLVDDPKRIAW 150

Query: 146 RYAKTWLVLDVISIIPSELARSILPPSAQAYGYFNMLRLWRLRRVSSMFARMEKDRHFNY 205
           RY  TWL+ DV+S +P EL  S+L  + Q YG F+MLRLWRL RVS  FAR+EKDR +NY
Sbjct: 151 RYTSTWLIFDVVSTVPYELFGSLLHNTIQGYGIFSMLRLWRLHRVSKCFARLEKDRKYNY 210

Query: 206 FWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARYVTAIYW 265
           FW+RC KL+ V LF VH   C  Y I + + DP   ++ L   ++ ++SL  RYVTA+YW
Sbjct: 211 FWIRCTKLLLVSLFVVHCGACFCYSIAAHYPDPSMTFMALAEANWKQKSLLIRYVTAMYW 270

Query: 266 SIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTRKFRDTI 325
           SI T +TTGYGD+H  N  E  FI+ YM F LGL AY+IGNMTNLVVH TSRTR FRDTI
Sbjct: 271 SITTFSTTGYGDIHGNNAEERAFILFYMIFNLGLLAYIIGNMTNLVVHVTSRTRNFRDTI 330

Query: 326 QAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAHHLFYSL 385
           QAAS FAQRN LP+ LQEQM+AHL L++RTD EGLQQQE +DSLPKAIRSSI+H+LFY +
Sbjct: 331 QAASAFAQRNNLPLGLQEQMVAHLSLRYRTDSEGLQQQEIIDSLPKAIRSSISHYLFYEV 390

Query: 386 VDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLIIQRNGI 445
           VD  YLF+G+S DL+FQLV+EMKAEYFPPKED+IL+NEAPSDFYI+VTG  D+I + NG+
Sbjct: 391 VDKTYLFHGISNDLLFQLVSEMKAEYFPPKEDVILRNEAPSDFYIMVTGAVDIIARVNGV 450

Query: 446 EEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGDGTIIMD 505
           ++++GEA+TG V GE+GVLCY+PQLFTVRT RLSQLLRLNR++F NLVQA+VGDG IIM+
Sbjct: 451 DQVVGEAQTGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTAFLNLVQANVGDGAIIMN 510

Query: 506 NLLKHLKEIKDPIMEEIVHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQLLKRGSQPNE 565
           NLL+HLK+  DP+M+ I+ ET   L Q  M++PL+L +AA  GDDLLLHQLLKRGS PNE
Sbjct: 511 NLLQHLKDSTDPVMKGILAETELMLAQGKMDLPLSLCFAAARGDDLLLHQLLKRGSNPNE 570

Query: 566 VDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKHESIVKLLMD 625
            D  +G+TALH+AA++G+++CVVLLLE+GADPN RD EG+VPLW+AI G+HE   KLL +
Sbjct: 571 TD-KNGRTALHIAASKGSQYCVVLLLEHGADPNIRDSEGSVPLWEAIIGRHEENAKLLSE 630

Query: 626 NRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAVCEGNSEMVK 685
           N A +S   V  F+C A  QN+++ L+ IV+ GGD++LS  NGTTALH AV EGN E+V+
Sbjct: 631 NGATLSFDTVGYFSCLAVGQNNLNALKDIVKYGGDISLSDVNGTTALHRAVSEGNLEIVQ 690

Query: 686 FLLEQGADIDKPDVHGWTPRALADHQGHEEIKELFSGKQSMNKPSSVVHIPQIPEPNYLR 745
           FLLE+GAD+DKPDV+GWT RALA+HQGHE+IK LF  ++ + +    + +   PE   L 
Sbjct: 691 FLLEKGADMDKPDVYGWTARALAEHQGHEDIKALFYNQRPVER--KTILVSGTPEIKPLM 750

Query: 746 KFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFMSTINNGERDVLQPT 805
           K  SE  M       +M P+  +       +RR+ +N++NSL G MS    G+       
Sbjct: 751 KHSSEPVMTHHHSREAMPPLARA-----VSQRRKLSNFKNSLFGIMSAAKTGDEG---GA 810

Query: 806 SINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFGISVTKILTQ 865
           S     S+GV   Y ARVT+S   +   +GK+V LP S++EL+EIG KK G   TKIL++
Sbjct: 811 STRTGISEGVGGVYPARVTIS--GEASSSGKVVKLPDSLEELIEIGEKKLGFVATKILSR 870

Query: 866 EGAEVEDVNLLRDGDRLVLV 882
           EGAE++D+ ++RDGD L+L+
Sbjct: 871 EGAEIDDIRIIRDGDFLLLL 877

BLAST of Lcy01g017010 vs. TAIR 10
Match: AT4G22200.1 (potassium transport 2/3 )

HSP 1 Score: 562.4 bits (1448), Expect = 6.6e-160
Identity = 338/872 (38.76%), Postives = 480/872 (55.05%), Query Frame = 0

Query: 18  GRDQEREIEQLSRDGSQYSLTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETFLI 77
           GR++E +   LS +    +L+  ILPPLGV       ++    IISP D  YR WE +++
Sbjct: 28  GREEEYDASSLSLN----NLSKLILPPLGVASYNQNHIRSSGWIISPMDSRYRCWEFYMV 87

Query: 78  VLVLYTAWVCPFEFGFLKTT-GGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEP 137
           +LV Y+AWV PFE  FL ++    L + DN+V+  FA+DIVLTFFVAY+D+ T LLV EP
Sbjct: 88  LLVAYSAWVYPFEVAFLNSSPKRNLCIADNIVDLFFAVDIVLTFFVAYIDERTQLLVREP 147

Query: 138 KLIAMRYAKTWLVLDVISIIPSELARSILPPSAQ---AYGYFNMLRLWRLRRVSSMFARM 197
           K IA+RY  TW ++DV S IP +    ++  ++          +LR WRLRRV  +F R+
Sbjct: 148 KQIAVRYLSTWFLMDVASTIPFDAIGYLITGTSTLNITCNLLGLLRFWRLRRVKHLFTRL 207

Query: 198 EKDRHFNYFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWA 257
           EKD  ++YFW+RC +L+ V LF VH AGC +YLI   +    K W T    ++ + SL  
Sbjct: 208 EKDIRYSYFWIRCFRLLSVTLFLVHCAGCSYYLIADRYPHQGKTW-TDAIPNFTETSLSI 267

Query: 258 RYVTAIYWSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSR 317
           RY+ AIYWSI T+TT GYGDLHA N  EM FI  YM F LGL AYLIGNMTNLVV GT R
Sbjct: 268 RYIAAIYWSITTMTTVGYGDLHASNTIEMVFITVYMLFNLGLTAYLIGNMTNLVVEGTRR 327

Query: 318 TRKFRDTIQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSI 377
           T +FR++I+AASNF  RN+LP RL++Q+LA++CL+F+   E L QQ  +D LPK+I  SI
Sbjct: 328 TMEFRNSIEAASNFVNRNRLPPRLKDQILAYMCLRFKA--ESLNQQHLIDQLPKSIYKSI 387

Query: 378 AHHLFYSLVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTAD 437
             HLF   V+  YLF GVS +++  LV++MKAEY PP+ED+I+QNEAP D YI+V+G  +
Sbjct: 388 CQHLFLPSVEKVYLFKGVSREILLLLVSKMKAEYIPPREDVIMQNEAPDDVYIIVSGEVE 447

Query: 438 LIIQRNGIEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASV 497
           +I      E ++G  + GD+ GE+G LC +PQ +T +T  LSQLLRL  S     +Q   
Sbjct: 448 IIDSEMERESVLGTLRCGDIFGEVGALCCRPQSYTFQTKSLSQLLRLKTSFLIETMQIKQ 507

Query: 498 GDGTIIMDNLLKHLKEIKDPIMEEI-VHETAQSLTQSGMEMPLNLHYAAESGDDLLLHQL 557
            D   ++ N L+H K++ +  + ++   +  ++       +  NL     +G+  LL +L
Sbjct: 508 QDNATMLKNFLQHHKKLSNLDIGDLKAQQNGENTDVVPPNIASNLIAVVTTGNAALLDEL 567

Query: 558 LKRGSQPNEVDGSSGKTALHLAAARGNEHCVVLLLENGADPNRRDFEGNVPLWQAIRGKH 617
           LK    P+  D S GKT LH+AA+RG E CV++LL++G + + RD  GN  LW+AI  KH
Sbjct: 568 LKAKLSPDITD-SKGKTPLHVAASRGYEDCVLVLLKHGCNIHIRDVNGNSALWEAIISKH 627

Query: 618 ESIVKLLMDNRANISSGDVAQFACAAAEQNSIDMLQSIVQCGGDVTLSRSNGTTALHLAV 677
             I ++L    A           C AA+QN++++++++++ G +V     +G TAL +A+
Sbjct: 628 YEIFRILYHFAAISDPHIAGDLLCEAAKQNNVEVMKALLKQGLNVDTEDHHGVTALQVAM 687

Query: 678 CEGNSEMVKFLLEQGADIDKPDVHG-WTPRALADHQGHEEIKELFSGKQSMNKPSSVVHI 737
            E   +MV  L   GAD+   + H  +TP                               
Sbjct: 688 AEDQMDMVNLLATNGADVVCVNTHNEFTPL------------------------------ 747

Query: 738 PQIPEPNYLRKFQSESNMPRFSQDYSMTPVRESNYSDMPPRRRRSNNYQNSLVGFMSTIN 797
                   LR  + E    R          R S Y   P  RR                 
Sbjct: 748 ------EKLRVVEEEEEEER---------GRVSIYRGHPLERR----------------- 797

Query: 798 NGERDVLQPTSINFSNSQGVKVAYQARVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKF 857
             ER                          SC E    AGKL+LLP S+ +L +I  +KF
Sbjct: 808 --ER--------------------------SCNE----AGKLILLPPSLDDLKKIAGEKF 797

Query: 858 GI--SVTKILTQEGAEVEDVNLLRDGDRLVLV 882
           G   S T +  ++GAE++ + ++RD D+L  V
Sbjct: 868 GFDGSETMVTNEDGAEIDSIEVIRDNDKLYFV 797

BLAST of Lcy01g017010 vs. TAIR 10
Match: AT4G18290.1 (potassium channel in Arabidopsis thaliana 2 )

HSP 1 Score: 557.0 bits (1434), Expect = 2.8e-158
Identity = 275/488 (56.35%), Postives = 357/488 (73.16%), Query Frame = 0

Query: 25  IEQLSRDGSQYS--LTGGILPPLGVNGRKNRRVKLRPHIISPFDYHYRLWETFLIVLVLY 84
           +E+ + D  ++S  L+  +LP LG   R N+  KLR HIISPFD  +R WE +L++LV+Y
Sbjct: 15  VEEYNMDTFKHSSFLSADLLPSLG--ARINQSTKLRKHIISPFDPRFRGWEMWLVILVIY 74

Query: 85  TAWVCPFEFGFLKTTGGVLAVIDNVVNGIFAIDIVLTFFVAYLDKSTYLLVDEPKLIAMR 144
           +AW+CPFEF F+      L +IDN+VNG FAIDI+LTFFVAYLD  +YLLVD+PK IA+R
Sbjct: 75  SAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLVDKPKKIAIR 134

Query: 145 YAKTWLVLDVISIIPSELARSILPPSAQAYGY--FNMLRLWRLRRVSSMFARMEKDRHFN 204
           Y  TW   DV S  P +    +   +    G+   +MLRLWRLRRVSS+FAR+EKD  FN
Sbjct: 135 YLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFARLEKDIRFN 194

Query: 205 YFWVRCAKLIFVCLFTVHFAGCCFYLIGSDFKDPKKVWLTLFYEDYNKESLWARYVTAIY 264
           YFW RC KLI V LF VH AGC  YLI   + DP K W+   Y ++ + S+W+RYVTA+Y
Sbjct: 195 YFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSVWSRYVTALY 254

Query: 265 WSIATLTTTGYGDLHAVNDREMTFIICYMFFILGLQAYLIGNMTNLVVHGTSRTRKFRDT 324
           WSI TLTTTGYGDLHA N REM F + +M F LG  +YLIGNMTNLVVH TSRTR FRDT
Sbjct: 255 WSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWTSRTRNFRDT 314

Query: 325 IQAASNFAQRNQLPVRLQEQMLAHLCLKFRTDLEGLQQQETVDSLPKAIRSSIAHHLFYS 384
           ++AAS FA RNQLP  +Q+QML+H+CLKF+T  EGL+QQE ++ LPKAIRSSIA++LF+ 
Sbjct: 315 VRAASEFASRNQLPPNIQDQMLSHICLKFKT--EGLKQQEALNGLPKAIRSSIANYLFFP 374

Query: 385 LVDSAYLFNGVSTDLIFQLVTEMKAEYFPPKEDIILQNEAPSDFYIVVTGTADLIIQRNG 444
           +V + YLF+GVS + +FQLV+++ AEYFPP+ED+ILQNEAP+D YI+V+G  D  +    
Sbjct: 375 IVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVDFTVYVGE 434

Query: 445 IEEIIGEAKTGDVIGEIGVLCYQPQLFTVRTSRLSQLLRLNRSSFFNLVQASVGDGTIIM 504
            +++ G+A  GD  GEIGVLCY PQ FTVRT+ LSQ+LR+++ S  + ++A V DG +IM
Sbjct: 435 EDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHVEDGRVIM 494

Query: 505 DNLLKHLK 509
           +NL   L+
Sbjct: 495 NNLFMKLR 498

HSP 2 Score: 61.2 bits (147), Expect = 4.8e-09
Identity = 27/64 (42.19%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 818 RVTLSCPEKGEVAGKLVLLPRSIQELLEIGAKKFGISVTKILTQEGAEVEDVNLLRDGDR 877
           RVT+    + +   KL++LP SI+ELL +  +KFG S TK+   E AE++D +++RDGD 
Sbjct: 629 RVTIHLKSRDKDLSKLIILPASIEELLRLAGEKFGYSFTKVTNAENAEIDDEDVIRDGDH 688

Query: 878 LVLV 882
           L ++
Sbjct: 689 LYIL 692