Lcy01g016840 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g016840
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr01: 38450424 .. 38453378 (-)
RNA-Seq Expression	Lcy01g016840
Synteny	Lcy01g016840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGGTGAAAGATCGATTATCGGCCTAGTTCTTCTTCGGTGCGTCTCTCCAGATCTCAACTTTGGTCTCCTCCTCTTGTTCAGTTCTGGAAAATTAATGTTGATGCAGCCTGGGATGCCTCTTCTACTGGCATCAGAGTGATTTGCCGGGATGATAGGGGGAGAATTTTGGGTGCCTGTTGTCAATCTCTAAATTCTTCTTTCCCTCTTCCCATGACGGAACTGAAGGCTATCATGAAAGGGGTAGAGTTTGCATCTTCCATGGGTGGGAGTAAATTGATTGTGGAGACAGATTGCCTTCAAACTTATAACTTGCTGAGTCAAGATATTGAAATCTGGAATGAAGTTGGAACTCTGGTAGATAATATTCGAAGAAGGTCTTTGTCTGGTGAGGAAATTGTTTTTTCATATGTTCCTCGTGAGTGTAATTCAATAGCAGATGCTATTGCTAAGAGAGCCAAGCGTGAAAAGTGTAATCTTGAGTGGAGAGATGATTTTCCACAGTGGCTTGTATTCCTGGTCAGTTGACCGTTTCCTTTTGCCCTTGGTGGCGTTATACAGTGTTTTTTCTTTAAAAAAAAAAGGGAAGTATATGTGCTTTTATTTTAATAGAGATGGATAAAAAAGAATTAATGCATTTGACAAATTTTCATATATGTATAAGTATAATTATAAACTATAAATTCTAGACATGTATTAGAATTTTTGATGTGTGTGTGTTTGTTTTAAAGAAAAAAGATGTTATATGTATATAAACTAATTGTAAGATATTATGTTTGATCATTTACTTTATTTTGTAGTTTACCCTAGTGAATTTTATAACACTCTCTTGACACATGTGATTATTTTATTTTATTTTTTATAACAAGGAAATGATTTTAGTTTATCATGACACGATTCAAAAAATAACTAGTAAATAAAAGAGAAAATTAATCTTAGATTTCTATCTTTGCTCATTTGGTAAATTCTTAAAACTAATTTATAAAACCTTGAATGTGTTATTGTAGTTTCAAATTCTATAATGATTATCTTCTATATATTTGTCAATCTAGCTGATATAAGATGAAAATTTCCTCGATAACTTTTTAATTATGCAGCAAGCTGATACGCAAATTGCGAACCGCAAGTGTACGGACCCAAGTTATAATATAGTGATTTAAAATCGAATGTCGTCCTCTGGATTGGTTTTCTAGTTCCCCTAATTATTAAAGGTATATTTGCCCAATTTTATTTAGAGAAAATAAGCGAGAAATATGCAAATCAATTAAAAACTAATCAAAGCAATAAAATGTGGAAACTCAATGGAGAAAGGCCTTAGGGAATTGATTTCACCTATTAGATGCATGAATTAATTTAACGAATAATTATGAACAAATTCGTAACAAGTTTATAAACCAAGGATTATGAATAATTAAAAGCCTCTCCCGAGTGCAATTAATCCTTTAAGCGAATTAAGAAATTGATATCCCTATCTAATTTCAACAATAAGCCTAAGCAATAAGAACAATCCTCATGACGATTTCTCAAGCAATTCCAAATCTCCCGATTAACGAATTACCTAAGTCATGCAATTGAGAGTCTAATCTAAACTACCCTCTCCCGAGCGATTAGTTCAGAAACAACAACATACAATCTGTGATCAAGAGATTATAAGCATTAATCAAACAATTGCATAAATAAACCGCGTTACGAAACGTCCTAAAAAACCGTAAATTAATCCATACAAACTAATGGCTACATCAAGCCCCTAAGAAACGAAATCTAGCTACTCATGATCTCTAAAATACAAGCGAAATTTGTCCCAAACATGCTTCAAATCAATACAAGAAATAAGGGAGAAAGATTAAACTCGTTGGCAGTGATTCCCGTGCGTTCCCGTCACGAACCTCTCGATCCAAGCTCCCAAAATCAAGCTCCCTCGCTCTCTGCCTTGTTCTGTCCGTTTTCTCTCTCTAGAAGTCGTTTTTCTCTCCCCAAAACAGCTCCCCCTTGACGAAAGCATCGCAAAATATAAATACTGCTGATTCTGTGTGACAGCGTCGAGACGCTGAGGACATAGCGTCGCGACGCTGTCGCGCGTACGCCCTAATTAAATCTGGAAAGTTCTCAGCGTCGAGACGCTGTGGAGGTAGCGTCTCGACTCTGCCTCGAATTTGCGCGCGTGCTGACTTGCGCCCAGCGTTGAGACGCTCCAATGGCTAGCGTCTCAACGCTAGGCCTTTAATTGATCTTCCTTTTCTATTTTCTTGGCCCGAACTTATTCTTTAGCTCCTAAATCATCGGTAAAGCTCAAAAATGTCGATTTCCATTGAAATACTTGATTCCTACAAAACAAAACCAAATAGAGATAAAAATAGCCAAATAATACTCAAGACTAACGATAAAATCATCTAAAATATGGCACAATAAATTGTGCTATCACAAGCATAAGATGGACCAAAAGTTAGTACGATTTATAGGCCAATTGTCACACACTATTTTTGGACGACATTCAAAAACACGTGAGAAGAAGATATTTTATATGGATTTCACATACAATGGCCGACAAGCGACGAATTGACTAGCTCACACCTTGGCCGTCTACGCTCGAACCCAACACCACACTGAATATTCAAACACTATTTCAAATGCACTTTCAGAAATACCTCGTCATAAGATGTTGTTGTAACACTATTAACTTCATTTAATTTTGTTTTGAATTACTAAAAACAATTACTACAAACTAATATAAATTAATTACATAAACTAAAATTATAAGCCATTGTGACATTAGTCATTAATGATTAAATAACTTAGTGAATGTATGTGAAATCCATATATATTGCTAATAGCATTAGCAATATATTTTATAGCCATGGGAGGGGCAATTATTGGAGCTGCAAAGTGCAAAAAACAAGCTCATGATTGGCAAAAGCGTCGTCGTTTTCCTCGAGCTTTTCCGACAACTTTCTGA

mRNA sequence

ATGAGAGCCTGGGATGCCTCTTCTACTGGCATCAGAGTGATTTGCCGGGATGATAGGGGGAGAATTTTGGGTGCCTGTTGTCAATCTCTAAATTCTTCTTTCCCTCTTCCCATGACGGAACTGAAGGCTATCATGAAAGGGGTAGAGTTTGCATCTTCCATGGGTGGGAGTAAATTGATTGTGGAGACAGATTGCCTTCAAACTTATAACTTGCTGAGTCAAGATATTGAAATCTGGAATGAAGTTGGAACTCTGGTAGATAATATTCGAAGAAGGTCTTTGTCTGGTGAGGAAATTGTTTTTTCATATGTTCCTCGTGAGTGTAATTCAATAGCAGATGCTATTGCTAAGAGAGCCAAGCGTGAAAAGTCCATGGGAGGGGCAATTATTGGAGCTGCAAAGTGCAAAAAACAAGCTCATGATTGGCAAAAGCGTCGTCGTTTTCCTCGAGCTTTTCCGACAACTTTCTGA

Coding sequence (CDS)

ATGAGAGCCTGGGATGCCTCTTCTACTGGCATCAGAGTGATTTGCCGGGATGATAGGGGGAGAATTTTGGGTGCCTGTTGTCAATCTCTAAATTCTTCTTTCCCTCTTCCCATGACGGAACTGAAGGCTATCATGAAAGGGGTAGAGTTTGCATCTTCCATGGGTGGGAGTAAATTGATTGTGGAGACAGATTGCCTTCAAACTTATAACTTGCTGAGTCAAGATATTGAAATCTGGAATGAAGTTGGAACTCTGGTAGATAATATTCGAAGAAGGTCTTTGTCTGGTGAGGAAATTGTTTTTTCATATGTTCCTCGTGAGTGTAATTCAATAGCAGATGCTATTGCTAAGAGAGCCAAGCGTGAAAAGTCCATGGGAGGGGCAATTATTGGAGCTGCAAAGTGCAAAAAACAAGCTCATGATTGGCAAAAGCGTCGTCGTTTTCCTCGAGCTTTTCCGACAACTTTCTGA

Protein sequence

MRAWDASSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCLQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKREKSMGGAIIGAAKCKKQAHDWQKRRRFPRAFPTTF

Homology

[Close]

BLAST of Lcy01g016840 vs. ExPASy TrEMBL
Match: A0A2P6R2K8 (Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0437761 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.9e-10
Identity = 41/115 (35.65%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 7   SSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCL 66
           +  G+  + RD+ GR +GA  Q L+ + P    EL AI  G+EFA   G S L+VE DCL
Sbjct: 12  AEVGVGAVFRDNTGRFVGAVGQRLSFNVPPRTAELFAIKIGLEFALEKGWSALVVECDCL 71

Query: 67  QTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKR 122
           +  NLLS++ E  +E G +   ++R  +  +     + PRE N  A  +A+   R
Sbjct: 72  EVVNLLSKEEECMSEDGVVAWEVQRLLVRFQHARIVFGPREVNRAAHMVAQHVAR 126

[Close]

BLAST of Lcy01g016840 vs. ExPASy TrEMBL
Match: A0A2P6SD65 (Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0339311 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.5e-09
Identity = 40/115 (34.78%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 7   SSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCL 66
           +  G   + R +RGR +GA  Q L+ + P    EL AI  G+EFA   G S L+VE DCL
Sbjct: 12  AQVGAGAVFRHNRGRFVGAVGQRLSFNIPPRTAELFAIKIGLEFALEKGWSALVVECDCL 71

Query: 67  QTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKR 122
           +  NLLS++ E  +  G +   ++R  +  +     + PRE N  A  +A+   R
Sbjct: 72  EVVNLLSREEECMSADGVVAWEVQRLLVRFQHARIVFSPREVNRAAHMVAQHVAR 126

[Close]

BLAST of Lcy01g016840 vs. ExPASy TrEMBL
Match: A0A5B6VSY9 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_022621 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.5e-08
Identity = 35/115 (30.43%), Postives = 63/115 (54.78%), Query Frame = 0


Query: 7    SSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCL 66
            +S  + V+ R+DRG I+GAC   +N +    + E +A  + + FA  MG  K++VE D L
Sbjct: 946  NSAIVGVVGRNDRGLIMGACTYQINDAADAFVAESRACERAILFAIDMGFRKIMVEGDSL 1005

Query: 67   QTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKR 122
                 +  +++  + +  ++ NIR  +   E+I FS++PR+ N +A  +A    R
Sbjct: 1006 TVIKKIRAELDDRSVIRPIIRNIRMLAKGFEQISFSFIPRKGNRVAHTLAAEGHR 1060

[Close]

BLAST of Lcy01g016840 vs. ExPASy TrEMBL
Match: A0A6P5RZ31 (uncharacterized protein LOC110751579 OS=Prunus avium OX=42229 GN=LOC110751579 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 6.0e-08
Identity = 34/110 (30.91%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 10  GIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCLQTY 69
           G+  + R+  G ++GA    +   F   +TEL AI +G++F    G   L+VETD     
Sbjct: 104 GLGAVVRNVHGDLMGAISVPITGCFSAKVTELLAIREGLQFTWETGYDSLVVETDAKNAI 163

Query: 70  NLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRA 120
           N +    E +   G ++++I   S + + + F + PR CN++AD +AK A
Sbjct: 164 NDIISGTEAFGVAGGIINDIHMLSRNFDSLSFVFSPRLCNTVADRLAKFA 213

[Close]

BLAST of Lcy01g016840 vs. ExPASy TrEMBL
Match: A0A7N2RC67 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 6.0e-08
Identity = 40/115 (34.78%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 6   ASSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDC 65
           +SS GI VI RD RG  +GA       S  + + E  A  + V FA  +G  ++I E D 
Sbjct: 126 SSSAGIGVIIRDSRGDAIGALSVPTPLSTSVAVMEALACRRAVLFAKEIGLGQVIFEGDS 185

Query: 66  LQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAK 121
                 + Q      E G ++D+IR   +  + I F++V R CN++ADA+ K+AK
Sbjct: 186 AVVIQAVIQGNSASAEYGNIIDDIRILVVDFDFIHFNHVKRNCNAVADALTKKAK 240

[Close]

BLAST of Lcy01g016840 vs. NCBI nr
Match: XP_042985441.1 (uncharacterized protein LOC122314103 [Carya illinoinensis])

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-10
Identity = 41/116 (35.34%), Postives = 62/116 (53.45%), Query Frame = 0


Query: 4   WDASSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVET 63
           +D    G+ VI RDD+G +L AC +           E  A+++G+ F +  G SKLI+E+
Sbjct: 46  YDQQKAGVGVILRDDKGEVLVACSKLEKEMISSEFIEATAMLRGLHFCAQWGVSKLILES 105

Query: 64  DCLQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRA 120
           DCL   N L  + E + E+G L+ +IRR     +E+  S+V R  N     +AK A
Sbjct: 106 DCLVLVNDLLSESENFTELGVLMQDIRRMLNGFQEVKVSHVNRLGNEAVHRLAKHA 161

[Close]

BLAST of Lcy01g016840 vs. NCBI nr
Match: PRQ40599.1 (putative ribonuclease H-like domain-containing protein [Rosa chinensis])

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-09
Identity = 41/115 (35.65%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 7   SSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETDCL 66
           +  G+  + RD+ GR +GA  Q L+ + P    EL AI  G+EFA   G S L+VE DCL
Sbjct: 12  AEVGVGAVFRDNTGRFVGAVGQRLSFNVPPRTAELFAIKIGLEFALEKGWSALVVECDCL 71

Query: 67  QTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKR 122
           +  NLLS++ E  +E G +   ++R  +  +     + PRE N  A  +A+   R
Sbjct: 72  EVVNLLSKEEECMSEDGVVAWEVQRLLVRFQHARIVFGPREVNRAAHMVAQHVAR 126

[Close]

BLAST of Lcy01g016840 vs. NCBI nr
Match: XP_030970592.1 (uncharacterized protein LOC115990972 [Quercus lobata])

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-09
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 5   DASSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETD 64
           +A    I V+ +D RG  +GA   S+  +  +   E  A  K +EFA  +G  ++I E D
Sbjct: 175 EAHLPSISVVVQDWRGEFVGALSSSMLLTHSVVEMEALACRKAIEFAIEIGLQRVIFEGD 234

Query: 65  CLQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAKREKS 124
                N L+Q+    +  G ++++IR  +L  E  VF++  R CNS+ADAIAK+AK  +S
Sbjct: 235 SAMVINALNQNNVGLSSYGAVIEDIRSLALVFESCVFAHTSRVCNSVADAIAKKAKAFRS 294

[Close]

BLAST of Lcy01g016840 vs. NCBI nr
Match: XP_042974894.1 (uncharacterized protein LOC122306534 [Carya illinoinensis])

HSP 1 Score: 72.8 bits (177), Expect = 3.0e-09
Identity = 41/117 (35.04%), Postives = 67/117 (57.26%), Query Frame = 0


Query: 5   DASSTGIRVICRDDRGRILGACCQSLNSSFPLP-MTELKAIMKGVEFASSMGGSKLIVET 64
           D +  G+ V+ RD  GR+L AC +S   + P P + E  A+++G++F    G SK+++ET
Sbjct: 626 DFNKAGVGVVLRDQAGRVLVACSKS-EPAVPNPEIIEATAMLRGLQFGLQWGTSKVLMET 685

Query: 65  DCLQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRAK 121
           DCL     L+   E   E   ++  I+R  L  +EI F +V R+ N++A  +AK A+
Sbjct: 686 DCLFLVKALNSHSENLTEFAYVLAEIKRLMLYFQEISFVHVSRQGNNVAHCLAKYAR 741

[Close]

BLAST of Lcy01g016840 vs. NCBI nr
Match: XP_024046567.1 (uncharacterized protein LOC112100924 [Citrus clementina] >XP_024950779.1 uncharacterized protein LOC112497228 [Citrus sinensis])

HSP 1 Score: 72.4 bits (176), Expect = 3.9e-09
Identity = 37/115 (32.17%), Postives = 63/115 (54.78%), Query Frame = 0


Query: 5   DASSTGIRVICRDDRGRILGACCQSLNSSFPLPMTELKAIMKGVEFASSMGGSKLIVETD 64
           D  S G+ V+ R+ RG++L A  +S+N    + + E  AI  G+E A  +  S +I+ETD
Sbjct: 23  DRKSAGLGVVIRNHRGQVLAAAVKSMNFQGNIAIAEAYAIKWGMEVAKDLSLSSVIIETD 82

Query: 65  CLQTYNLLSQDIEIWNEVGTLVDNIRRRSLSGEEIVFSYVPRECNSIADAIAKRA 120
           C    +L ++ +    E+   + +I + +   + I F +V R+CN  A  +AKRA
Sbjct: 83  CKNVADLANKKVSNMTEIWWTMSDIHKSTQDFQLISFQHVLRKCNGFAHFLAKRA 137

The following BLAST results are available for this feature:

BLAST of Lcy01g016840 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy01g016840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2P6R2K8	4.9e-10	35.65	Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=7464...	[more]
A0A2P6SD65	5.5e-09	34.78	Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=7464...	[more]
A0A5B6VSY9	3.5e-08	30.43	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_022621 PE=4 SV=1	[more]
A0A6P5RZ31	6.0e-08	30.91	uncharacterized protein LOC110751579 OS=Prunus avium OX=42229 GN=LOC110751579 PE...	[more]
A0A7N2RC67	6.0e-08	34.78	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Lcy01g016840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_042985441.1	2.1e-10	35.34	uncharacterized protein LOC122314103 [Carya illinoinensis]	[more]
PRQ40599.1	1.0e-09	35.65	putative ribonuclease H-like domain-containing protein [Rosa chinensis]	[more]
XP_030970592.1	1.3e-09	35.00	uncharacterized protein LOC115990972 [Quercus lobata]	[more]
XP_042974894.1	3.0e-09	35.04	uncharacterized protein LOC122306534 [Carya illinoinensis]	[more]
XP_024046567.1	3.9e-09	32.17	uncharacterized protein LOC112100924 [Citrus clementina] >XP_024950779.1 unchara...	[more]

BLAST of Lcy01g016840 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 4..128 e-value: 1.7E-12 score: 49.4
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 7..119 e-value: 3.4E-22 score: 78.5
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 7..128
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 7..128
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 5..117 e-value: 5.57515E-20 score: 78.1248
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 10..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g016840.1	Lcy01g016840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity