Homology
BLAST of Lcy01g014450 vs. ExPASy Swiss-Prot
Match:
A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)
HSP 1 Score: 238.0 bits (606), Expect = 2.0e-61
Identity = 148/443 (33.41%), Postives = 235/443 (53.05%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M +++++SEEL++PSSPTP L+ +KLS LDQV + P++ Y D +
Sbjct: 1 MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRS 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
LK SLS VL+ +YPLAGR++ + +DCND GVPF+E RV +L + + ++LN
Sbjct: 61 ENLKRSLSTVLTQFYPLAGRIN-INSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 120
Query: 121 LVPF-------ELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAA 180
+PF E +I + V+++ FECG +G+C+SHKIADALS + +N W A
Sbjct: 121 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATC 180
Query: 181 RGVVEDLGRPHL-VAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESA 240
+ D+ +P+ + + FPP ++ + V +RFV + K+EA++ + A SA
Sbjct: 181 QEET-DIVQPNFDLGSHHFPPME-SIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSA 240
Query: 241 AMEGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFG 300
+ SRV+ + A ++ +F+ + + T + VN+R ++NPP PQ+A G
Sbjct: 241 T--EVKNSSRVQIVIAVIWKQFIDVTR--AKFDTKNKLVAAQAVNLRSRMNPPFPQSAMG 300
Query: 301 NYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFV 360
N T+ A D + S LV ++ +AKID E +++ Q+ L+ F
Sbjct: 301 ----NIATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYEAEPQELF- 360
Query: 361 KGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLK 420
+F+S CR YD DFGWG P + +P +NLV MD ++ DG +EA + +
Sbjct: 361 -------SFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDG-MEAWISMA 420
Query: 421 ESHMERLEADHEFLEFASTTGSD 436
E M L +D FL T S+
Sbjct: 421 EDEMSMLSSD--FLSLLDTDFSN 421
BLAST of Lcy01g014450 vs. ExPASy Swiss-Prot
Match:
Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)
HSP 1 Score: 229.2 bits (583), Expect = 9.1e-59
Identity = 146/432 (33.80%), Postives = 228/432 (52.78%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M ++E VSEEL+ PSSPTP +L+ YK+S LDQ+ + P + Y D +
Sbjct: 1 MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFP-EDLN 120
LK SLS VL+H+YPLAGR++ + +DCND GVPF+E RV+ +L + E L+
Sbjct: 61 QHLKQSLSKVLTHFYPLAGRIN-VNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 120
Query: 121 CLVPF------ELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAA 180
+P +++ +++ + V+++FFECG +G+ +SHKIAD LS + +N W A
Sbjct: 121 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 180
Query: 181 RGVVEDLGRPHLVAARLFPPRNAALYNTRN-SIARDR--VARRFVVEGSKVEAIREKYAE 240
RG E + +AAR FPP + NT + + D V +RFV + K+ A+R A+
Sbjct: 181 RGETEIVLPNFDLAARHFPP----VDNTPSPELVPDENVVMKRFVFDKEKIGALR---AQ 240
Query: 241 SAAMEGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNA 300
+++ ++ SRV+ + A+++ + + + A+ F+V VN+R ++NPPLP A
Sbjct: 241 ASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNK--FVVVQAVNLRSRMNPPLPHYA 300
Query: 301 FGNYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSAR 360
GN AE D + L+ +R + K E++ E++K +
Sbjct: 301 MGNIATLLFAAVDAEW--DKDFPDLIGPLRTSLEK---------TEDDHNHELLKGMTCL 360
Query: 361 FVKGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVH 420
+ +FTS CR YD DFGWG P A + P RN + MD +SGDG VEA +
Sbjct: 361 YELEPQELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDG-VEAWLP 410
Query: 421 LKESHMERLEAD 423
+ E M L +
Sbjct: 421 MAEDEMAMLPVE 410
BLAST of Lcy01g014450 vs. ExPASy Swiss-Prot
Match:
A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)
HSP 1 Score: 203.0 bits (515), Expect = 7.0e-51
Identity = 145/438 (33.11%), Postives = 225/438 (51.37%), Query Frame = 0
Query: 5 VEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIRNRLK 64
VE+VS+ +KPSSPTP +LRRY LS +DQ + + Y N P +I + LK
Sbjct: 6 VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYN--PSHESSKIGDLLK 65
Query: 65 SSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVV--SKSFPEDLNCLV 124
+SLS L YY AGR+ D+ IDCND+GV F+E R+ +++D++ KSF DL ++
Sbjct: 66 NSLSKTLVSYYQFAGRLIENDY-IDCNDEGVEFVEVRIHGRMNDILKRGKSFATDL--VL 125
Query: 125 PFELDRLDEIS-MGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDLG 184
P + L E S + VQL+ F+CG I +G SHK++D +S + WA++
Sbjct: 126 PTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKP 185
Query: 185 RPHLVAARLFPPRNAALYNTRNSIA-RDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 244
P L A +FPP + L + ++ ++ + + +RFV +E ++ K E G ++P
Sbjct: 186 TPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YGIQKP 245
Query: 245 SRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNAMT 304
SRVE ++A FL + + + VN+R L LPQN+ GN +
Sbjct: 246 SRVEVVTA-----FLCQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIFSFYFC 305
Query: 305 LPSAETLADGNCSGLVNEVRHQ--------IAKIDAEFLRQFQEENAQLEIIKATSARFV 364
+ + D S L++++R+ I+K + Q QE L+ + +S
Sbjct: 306 I--NDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLNISSL--- 365
Query: 365 KGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALP-FRNLVVFMDGKSGDGVVEALVHL 424
+ C +S CRFP+YD DFGWG P SP P ++L++ MD GDG +EAL+ +
Sbjct: 366 --DTYFC--SSWCRFPVYDIDFGWGKPI-LVSPFQPHVKDLILLMDSPEGDG-IEALITM 416
Query: 425 KESHMERLEADHEFLEFA 430
+E M E + E FA
Sbjct: 426 EEKKMAAFEKNEELRSFA 416
BLAST of Lcy01g014450 vs. ExPASy Swiss-Prot
Match:
I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)
HSP 1 Score: 198.0 bits (502), Expect = 2.2e-49
Identity = 146/462 (31.60%), Postives = 231/462 (50.00%), Query Frame = 0
Query: 5 VEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFT---ELEIRN 64
VEV+S+E +KP +PTP LR Y +S LDQ ++ VY P++ Y D T + +
Sbjct: 9 VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68
Query: 65 RLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNCL 124
LK SLS L H+YP+AGR+ + +DCND+G+ F E R+K ++ D + KS L+ L
Sbjct: 69 LLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFMMKS-DAHLSLL 128
Query: 125 VPFEL---DRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAA----AAR 184
+P E+ + + E + VQ+N F+CG + C+S+KIADA + + + A A R
Sbjct: 129 LPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIARR 188
Query: 185 G--VVEDLGRPHLV----AARLFPP----RNAALYNTRNSIARDR-VARRFVVEGSKVEA 244
G + +LV + LFPP + Y T++S + D+ V++RFV + +K+ +
Sbjct: 189 GSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKITS 248
Query: 245 IREKYAESAAMEGQ-RRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKL 304
REK + + RP+RVE +SA ++ +A+K SA + V H +N R K+
Sbjct: 249 AREKLQSLMHDKYKCHRPTRVEVVSALIW---KSAVK---SAPPGSISTVTHAMNFRKKM 308
Query: 305 NPPLPQNAFGNYYRNAMTLPSAETLADGN-----CSGLVNEVR---HQIAKIDAEFLRQF 364
+PPL +FGN + A T N S NE + +++ A R+
Sbjct: 309 DPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVALLRREI 368
Query: 365 QEENAQLEIIKATSARFVKGEIVSCA-------------FTSLCRFPLYDADFGWGSPAW 421
+ ++ +F+ G S A TS C+F Y+ADFGWG+P W
Sbjct: 369 DKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYEADFGWGTPVW 428
BLAST of Lcy01g014450 vs. ExPASy Swiss-Prot
Match:
Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)
HSP 1 Score: 192.6 bits (488), Expect = 9.4e-48
Identity = 142/449 (31.63%), Postives = 229/449 (51.00%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELE-I 60
+ +E E +S+ L+KPSSPTP +L RY LS+ DQ ++Y V + +P E+ I
Sbjct: 6 ISVETETLSKTLIKPSSPTPQSLSRYNLSYNDQ---NIYQTCVSVGFFYENPDGIEISTI 65
Query: 61 RNRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVS---KSFPE 120
R +L++SLS L YYP AG+V D+ I CNDDG+ F+E R++C+++D++ +S+
Sbjct: 66 REQLQNSLSKTLVSYYPFAGKVVKNDY-IHCNDDGIEFVEVRIRCRMNDILKYELRSYAR 125
Query: 121 DLNCLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAA--R 180
DL + D ++ VQL+ F+CG + + +SHK+AD + S + WAA+A
Sbjct: 126 DLVLPKRVTVGSEDTTAI-VQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAASACYL 185
Query: 181 GVVEDLGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAM 240
+ P LV+ +F PR + + +++ V + F+ +E ++ K E
Sbjct: 186 SSSHHVPTPLLVSDSIF-PRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVEF--- 245
Query: 241 EGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRL-----FLVCHTVNIRHKLNPPLPQN 300
G +P+RVE L+AF+ A + +S+A+ + F V +N+R L LPQN
Sbjct: 246 -GIEKPTRVEVLTAFL--SRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPILE--LPQN 305
Query: 301 AFGN----YYRNAMTLPSAETLADGNCSGLV-NEVRHQIAKIDAEFLRQFQEENAQLEII 360
+ GN Y+ + T+ + L + + LV NE+R + KI + E +
Sbjct: 306 SVGNLVSIYF--SRTIKENDYLNEKEYTKLVINELRKEKQKIKNLSREKLTYVAQMEEFV 365
Query: 361 KATSARFVKG--EIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGD 420
K+ + +I + S CRFP YD DFGWG P W +N VV MD GD
Sbjct: 366 KSLKEFDISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYPFGD 425
Query: 421 GV-VEALVHLKESHMERLEADHEFLEFAS 431
+EA+V ++ M E + + L+F S
Sbjct: 426 DYGIEAIVSFEQEKMSAFEKNEQLLQFVS 438
BLAST of Lcy01g014450 vs. ExPASy TrEMBL
Match:
A0A1S3BB10 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1)
HSP 1 Score: 740.7 bits (1911), Expect = 3.4e-210
Identity = 365/435 (83.91%), Postives = 391/435 (89.89%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGD-PKFTELEI 60
MKLE EV+S+E VKPSSPTPANLRRYK SFLDQVTADVYNPMVY +ELNGD FTE+EI
Sbjct: 1 MKLEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEI 60
Query: 61 RNRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLN 120
+N LK SLSAVLS YYPLAGRV+YADF IDCNDDGVPF+ETRVK KLHDV+SKSFPEDLN
Sbjct: 61 KNHLKHSLSAVLSDYYPLAGRVNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLN 120
Query: 121 CLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVED 180
CLVPFELDRL+EISMGVQLNFFECG IGLG+CVSHKIADALSFFS VN WA AARG VE
Sbjct: 121 CLVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEA 180
Query: 181 LGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRR 240
LGRPHLVAA+LFPPRNAA+YNT NSI RDRV RRFVVEG KVEAIR++YAE+A+MEGQRR
Sbjct: 181 LGRPHLVAAKLFPPRNAAVYNTGNSIVRDRVVRRFVVEGPKVEAIRKQYAENASMEGQRR 240
Query: 241 PSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNAM 300
P+RVEALSAF+YGRFLAAI DESSAQTDR FLVCHTVNIR KL PPLPQNAFGNYYRNAM
Sbjct: 241 PTRVEALSAFIYGRFLAAINDESSAQTDRFFLVCHTVNIRQKLYPPLPQNAFGNYYRNAM 300
Query: 301 TLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVSC 360
TLPSA TL D NCS L+NEVR Q+AKI+ EFLRQFQEENAQLE IKATSARF+KG+IVSC
Sbjct: 301 TLPSAATLTDANCSALINEVRLQVAKINPEFLRQFQEENAQLETIKATSARFLKGDIVSC 360
Query: 361 AFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMERL 420
AFTSLCR PLYDADFGWGSP WAASPALPF+NL V MDGKS DG V+ LVHLKESHMERL
Sbjct: 361 AFTSLCRLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERL 420
Query: 421 EADHEFLEFASTTGS 435
E D EFL+FAS T S
Sbjct: 421 EVDREFLKFASPTAS 435
BLAST of Lcy01g014450 vs. ExPASy TrEMBL
Match:
A0A6J1BS06 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1)
HSP 1 Score: 686.0 bits (1769), Expect = 1.0e-193
Identity = 345/436 (79.13%), Postives = 374/436 (85.78%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYE-LNGDPKFTELEI 60
MKLEVEV+S+E V+PSSPTP NLRRYK SFLDQVTADVYNPMVY YE L+G P F +EI
Sbjct: 1 MKLEVEVISKEFVRPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDGGPNFNAVEI 60
Query: 61 RNRLKSSLSAVLSHYYPLAGR-VDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDL 120
+NRLK+SLS VLS YYPLAGR + AD IDCNDDGVPF+E RVK L+DVV S P DL
Sbjct: 61 KNRLKNSLSQVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADL 120
Query: 121 NCLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVE 180
N L+PF+LD+L+EISMGVQ N FE G I L ICVSHKIADALSFFSVVNGWA AARG E
Sbjct: 121 NDLLPFQLDQLNEISMGVQFNVFELGGIALAICVSHKIADALSFFSVVNGWAVAARGASE 180
Query: 181 DLGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQR 240
LGRPH+ AA+LFPPRN +LYNTRNSI RDRVARRFV+EGSKVEAIR KYA+SAAMEGQR
Sbjct: 181 ALGRPHIAAAKLFPPRNVSLYNTRNSIVRDRVARRFVLEGSKVEAIRAKYADSAAMEGQR 240
Query: 241 RPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNA 300
RPSRVEALSAF+Y RFLAAI ES AQ DRLFLVCHTVNIR KL PPLPQ+AFGNYYR A
Sbjct: 241 RPSRVEALSAFIYSRFLAAINHESGAQPDRLFLVCHTVNIRQKLEPPLPQDAFGNYYRTA 300
Query: 301 MTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVS 360
+TLPS ETL D NCSGLVNEVRHQIAKIDAEFLRQFQEENAQLE IKATSARF+KGEIVS
Sbjct: 301 LTLPSPETLTDDNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLETIKATSARFMKGEIVS 360
Query: 361 CAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMER 420
A TSLCRFPLYDADFGWG PAWAASPALPF+NLVV MD KSGDG ++A+VHL E HM +
Sbjct: 361 FALTSLCRFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAK 420
Query: 421 LEADHEFLEFASTTGS 435
LEAD EFL+FAS T S
Sbjct: 421 LEADEEFLKFASPTAS 436
BLAST of Lcy01g014450 vs. ExPASy TrEMBL
Match:
A0A6J1DG29 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111019771 PE=3 SV=1)
HSP 1 Score: 544.3 bits (1401), Expect = 4.7e-151
Identity = 268/436 (61.47%), Postives = 326/436 (74.77%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M +E+EV+S+E++KP SPTPA+LRR +SFLDQVT DVYNPMVY Y D F +I
Sbjct: 1 MAVEIEVISKEMIKPFSPTPAHLRRCNMSFLDQVTTDVYNPMVYFYAPTAD--FDAAKIA 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
+ LK+SLS VL+ +YPLAGRV+Y +F IDC+D GVPFIETRV C+L DVV FP +LN
Sbjct: 61 DHLKNSLSHVLTEFYPLAGRVNYGEFFIDCDDGGVPFIETRVNCRLSDVVGTPFPGELNK 120
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
+PFELD+L+E+SMGVQLN FECG +G+GICVSHKI DALSFF+VVNGWAA RG VE L
Sbjct: 121 FLPFELDQLEEVSMGVQLNVFECGGVGVGICVSHKIGDALSFFTVVNGWAAFGRGEVEAL 180
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
R H + LFPP ALYNTRNSI R+RVA+R+ + G+ +EAIR KYAES A E QRRP
Sbjct: 181 -RGHFASGELFPPTTTALYNTRNSIFRERVAKRYEINGANIEAIRAKYAESDAFENQRRP 240
Query: 241 SRVEALSAFVYGRFLAAIKDESSAQTDRL-------FLVCHTVNIRHKLNPPLPQNAFGN 300
SRVE+LSAF+YGRF+AA+KDE S + RL +LVCH VNIR +L PP+P+ AFGN
Sbjct: 241 SRVESLSAFLYGRFIAAVKDECSDENGRLESEKTTTYLVCHAVNIRSRLEPPVPEYAFGN 300
Query: 301 YYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVK 360
YYR+ +PS E L D NC LV VR +IAKID +L+Q Q+ L+ +K TSARF
Sbjct: 301 YYRSTFAIPSQEILDDPNCYALVPHVRQEIAKIDTHYLKQIQDNRNFLDAMKKTSARFAA 360
Query: 361 GEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKE 420
G ++SC+FTSLCR P+YDADFGWG P W SPAL F+NL V +D K GDG V+ VHLK+
Sbjct: 361 GSLLSCSFTSLCRMPIYDADFGWGKPEWVGSPALIFKNLFVLVDKKDGDG-VDVYVHLKK 420
Query: 421 SHMERLEADHEFLEFA 430
HM RLEADH FL +A
Sbjct: 421 EHMARLEADHLFLNYA 432
BLAST of Lcy01g014450 vs. ExPASy TrEMBL
Match:
A0A0A0KPY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1)
HSP 1 Score: 528.1 bits (1359), Expect = 3.5e-146
Identity = 258/437 (59.04%), Postives = 327/437 (74.83%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M +E+E+VS+E +KPSSPTP +LRRY LSFLDQVT DVYNPMVY Y G T EI
Sbjct: 1 MAVEIEIVSKETIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSA--TPAEIS 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
+ LK+SLS VL+HYYPLAG+V+Y +F IDCND+GVPFIET++ C+L DV++ FP ++N
Sbjct: 61 DHLKTSLSHVLTHYYPLAGKVNYDEFFIDCNDNGVPFIETKINCRLSDVMNTPFPSEVNK 120
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
+PFELD+LDE+SMGVQLN FECG + +GICVSHKI+DALS F VVN WAA RG +++
Sbjct: 121 FLPFELDQLDEVSMGVQLNVFECGGVVIGICVSHKISDALSLFIVVNEWAAYCRG-EKEV 180
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
R HL +A LFPP LYNTR SI R RVARR+ ++G VE+IR KYAE +AME QRRP
Sbjct: 181 VRAHLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGENVESIRAKYAECSAMENQRRP 240
Query: 241 SRVEALSAFVYGRFLAAIKDESSAQTD--------RLFLVCHTVNIRHKLNPPLPQNAFG 300
SRVEALSAF+Y RF+AAIK SS++ + ++FLVCH+VNIR +L+PP+ AFG
Sbjct: 241 SRVEALSAFIYSRFIAAIKAVSSSELENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFG 300
Query: 301 NYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFV 360
NYYR +PS L D C LV + R++I KID ++L++ QE + L+ +K T+ +F
Sbjct: 301 NYYRTTFAVPSENILNDNYCYDLVKQARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFS 360
Query: 361 KGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLK 420
GE+VSC+FTSLCR P+YDADFGWG PAW +SPAL F+NL VF+D K GDG V+ VHLK
Sbjct: 361 TGELVSCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVFIDKKDGDG-VDIYVHLK 420
Query: 421 ESHMERLEADHEFLEFA 430
E HM + E D EFL++A
Sbjct: 421 EEHMNKFEVDEEFLKYA 433
BLAST of Lcy01g014450 vs. ExPASy TrEMBL
Match:
A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)
HSP 1 Score: 520.0 bits (1338), Expect = 9.5e-144
Identity = 256/437 (58.58%), Postives = 324/437 (74.14%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M ++++VS+E +KPSSPTP +LRRY LSFLDQVT DVYNPMVY + T EI
Sbjct: 1 MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTT--TPAEIA 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
+RLK+SLS VL+HYYPLAGR++Y +F IDCNDDGVPFIETRV C L DV++ FP +LN
Sbjct: 61 DRLKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNR 120
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
L+PFELD+LD++SMGVQLN FECG + +GICVSHKI+DALSFF VVNGWAA RG E L
Sbjct: 121 LLPFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEAL 180
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
R HL +A +FPP ++NTR SI R RV RR+ ++ + VE IR KYAE AME QRRP
Sbjct: 181 -RAHLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRP 240
Query: 241 SRVEALSAFVYGRFLAAIK-------DESSAQTD-RLFLVCHTVNIRHKLNPPLPQNAFG 300
SRVEALS F+Y RF+AAIK + S++T+ ++FLVCH+VNIR +L+PP+P AFG
Sbjct: 241 SRVEALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFG 300
Query: 301 NYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFV 360
NYYR PS E L D C LV +VR +I KI+ ++L++ QE + +E +K T+++F
Sbjct: 301 NYYRTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFF 360
Query: 361 KGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLK 420
GE+VSC+FTSLCR P+YD DFGWG P W +SPAL F+NL VF+D K GDG +E VHL
Sbjct: 361 TGELVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDG-IEIYVHLT 420
Query: 421 ESHMERLEADHEFLEFA 430
E HM++ E D EFL++A
Sbjct: 421 EEHMKKFEIDEEFLKYA 433
BLAST of Lcy01g014450 vs. NCBI nr
Match:
XP_038886246.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])
HSP 1 Score: 755.0 bits (1948), Expect = 3.6e-214
Identity = 369/432 (85.42%), Postives = 401/432 (92.82%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
MKLE+EV SEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVY Y+LN D FT+++I+
Sbjct: 40 MKLELEVTSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYFYQLNRDSNFTKIQIK 99
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
N LK++LSAVLS YYPLAGRV+YADFVIDCNDDG+PF+ETRVK LHDVVS+SFPEDLNC
Sbjct: 100 NHLKNTLSAVLSDYYPLAGRVNYADFVIDCNDDGIPFLETRVKFNLHDVVSRSFPEDLNC 159
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
LVPFELDRL+EISMGVQLNFFECG IGLG+CVSHKIADALSFFSVVN WA AARG E +
Sbjct: 160 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSVVNSWARAARGEAEAI 219
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
GRPHLVAA+LFPPRNAA+YNT NSI RDRVARRFV++GSKVEAIREKYAE AAMEGQRRP
Sbjct: 220 GRPHLVAAKLFPPRNAAVYNTGNSIVRDRVARRFVIDGSKVEAIREKYAERAAMEGQRRP 279
Query: 241 SRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNAMT 300
++VEALSAF+YGRFLAAI+DESSAQTDRLFLVCHTVNIR KL+PPLPQNAFGNYYRNAMT
Sbjct: 280 TKVEALSAFIYGRFLAAIEDESSAQTDRLFLVCHTVNIRQKLDPPLPQNAFGNYYRNAMT 339
Query: 301 LPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVSCA 360
LPSA+TLAD NC LVNEVR QIAKI+AEFLRQFQEENAQ+EIIKATSARF KGEIVSCA
Sbjct: 340 LPSAQTLADPNCCALVNEVRLQIAKINAEFLRQFQEENAQVEIIKATSARFAKGEIVSCA 399
Query: 361 FTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMERLE 420
FTSLCRFPLYDADFGWGSP WAASPALPF+NLVVFMD KSGDG V+A+VHLKESHM+R E
Sbjct: 400 FTSLCRFPLYDADFGWGSPLWAASPALPFKNLVVFMDTKSGDGAVDAIVHLKESHMKRFE 459
Query: 421 ADHEFLEFASTT 433
D EFL+FAS T
Sbjct: 460 VDCEFLKFASPT 471
BLAST of Lcy01g014450 vs. NCBI nr
Match:
XP_008444409.1 (PREDICTED: vinorine synthase-like [Cucumis melo])
HSP 1 Score: 740.7 bits (1911), Expect = 7.1e-210
Identity = 365/435 (83.91%), Postives = 391/435 (89.89%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGD-PKFTELEI 60
MKLE EV+S+E VKPSSPTPANLRRYK SFLDQVTADVYNPMVY +ELNGD FTE+EI
Sbjct: 1 MKLEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEI 60
Query: 61 RNRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLN 120
+N LK SLSAVLS YYPLAGRV+YADF IDCNDDGVPF+ETRVK KLHDV+SKSFPEDLN
Sbjct: 61 KNHLKHSLSAVLSDYYPLAGRVNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLN 120
Query: 121 CLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVED 180
CLVPFELDRL+EISMGVQLNFFECG IGLG+CVSHKIADALSFFS VN WA AARG VE
Sbjct: 121 CLVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEA 180
Query: 181 LGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRR 240
LGRPHLVAA+LFPPRNAA+YNT NSI RDRV RRFVVEG KVEAIR++YAE+A+MEGQRR
Sbjct: 181 LGRPHLVAAKLFPPRNAAVYNTGNSIVRDRVVRRFVVEGPKVEAIRKQYAENASMEGQRR 240
Query: 241 PSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNAM 300
P+RVEALSAF+YGRFLAAI DESSAQTDR FLVCHTVNIR KL PPLPQNAFGNYYRNAM
Sbjct: 241 PTRVEALSAFIYGRFLAAINDESSAQTDRFFLVCHTVNIRQKLYPPLPQNAFGNYYRNAM 300
Query: 301 TLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVSC 360
TLPSA TL D NCS L+NEVR Q+AKI+ EFLRQFQEENAQLE IKATSARF+KG+IVSC
Sbjct: 301 TLPSAATLTDANCSALINEVRLQVAKINPEFLRQFQEENAQLETIKATSARFLKGDIVSC 360
Query: 361 AFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMERL 420
AFTSLCR PLYDADFGWGSP WAASPALPF+NL V MDGKS DG V+ LVHLKESHMERL
Sbjct: 361 AFTSLCRLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERL 420
Query: 421 EADHEFLEFASTTGS 435
E D EFL+FAS T S
Sbjct: 421 EVDREFLKFASPTAS 435
BLAST of Lcy01g014450 vs. NCBI nr
Match:
XP_004143050.2 (stemmadenine O-acetyltransferase [Cucumis sativus] >KAE8652046.1 hypothetical protein Csa_018730 [Cucumis sativus])
HSP 1 Score: 716.1 bits (1847), Expect = 1.9e-202
Identity = 355/434 (81.80%), Postives = 380/434 (87.56%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
MKLE EV+S+ELVKPSSPTP NLRRYK SFLDQVTADVYNPMVY ++LNGD FTE+EI+
Sbjct: 1 MKLEFEVISKELVKPSSPTPPNLRRYKFSFLDQVTADVYNPMVYFFQLNGDSNFTEIEIK 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
LK+SLSAVLS YYPLAGRV+YADF IDCND GVPF+ETR+K LHDV S SFPEDLNC
Sbjct: 61 THLKTSLSAVLSDYYPLAGRVNYADFEIDCNDHGVPFLETRIKSTLHDVFSMSFPEDLNC 120
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
LVPFELDRL+E SMGVQLNFFECG IGLG+CVSHKIADALSFFSVVN WA AARG E L
Sbjct: 121 LVPFELDRLNETSMGVQLNFFECGGIGLGVCVSHKIADALSFFSVVNIWARAARGEAEAL 180
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
GR HLVAA+LFPPRNA +YNT NSI RDRVARRFVVEG KVEAIRE+YAE AAMEGQRRP
Sbjct: 181 GRAHLVAAKLFPPRNAGVYNTGNSIVRDRVARRFVVEGPKVEAIREQYAERAAMEGQRRP 240
Query: 241 SRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNAMT 300
+RVEALSAF+YGRFLAAI DESSAQTDRL LVCHTVNIR KL PPLPQNAFGNYYR MT
Sbjct: 241 TRVEALSAFIYGRFLAAINDESSAQTDRLSLVCHTVNIRQKLYPPLPQNAFGNYYRITMT 300
Query: 301 LPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVSCA 360
LPSA TL D NCS LVN+VR QIAKI+ EFLRQFQEENAQLEIIKATSA+F KG+IVSCA
Sbjct: 301 LPSAATLTDANCSALVNQVRLQIAKINPEFLRQFQEENAQLEIIKATSAKFHKGDIVSCA 360
Query: 361 FTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMERLE 420
FTSLCR PLYDADFGWG P WA SPALPF+NL V MDGK GDG V+ LVHLKESHMERL+
Sbjct: 361 FTSLCRLPLYDADFGWGPPDWAGSPALPFKNLFVLMDGKFGDGAVDVLVHLKESHMERLK 420
Query: 421 ADHEFLEFASTTGS 435
D EFL+FAS T S
Sbjct: 421 VDREFLKFASPTAS 434
BLAST of Lcy01g014450 vs. NCBI nr
Match:
XP_022132064.1 (vinorine synthase-like [Momordica charantia])
HSP 1 Score: 686.0 bits (1769), Expect = 2.1e-193
Identity = 345/436 (79.13%), Postives = 374/436 (85.78%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYE-LNGDPKFTELEI 60
MKLEVEV+S+E V+PSSPTP NLRRYK SFLDQVTADVYNPMVY YE L+G P F +EI
Sbjct: 1 MKLEVEVISKEFVRPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDGGPNFNAVEI 60
Query: 61 RNRLKSSLSAVLSHYYPLAGR-VDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDL 120
+NRLK+SLS VLS YYPLAGR + AD IDCNDDGVPF+E RVK L+DVV S P DL
Sbjct: 61 KNRLKNSLSQVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADL 120
Query: 121 NCLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVE 180
N L+PF+LD+L+EISMGVQ N FE G I L ICVSHKIADALSFFSVVNGWA AARG E
Sbjct: 121 NDLLPFQLDQLNEISMGVQFNVFELGGIALAICVSHKIADALSFFSVVNGWAVAARGASE 180
Query: 181 DLGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQR 240
LGRPH+ AA+LFPPRN +LYNTRNSI RDRVARRFV+EGSKVEAIR KYA+SAAMEGQR
Sbjct: 181 ALGRPHIAAAKLFPPRNVSLYNTRNSIVRDRVARRFVLEGSKVEAIRAKYADSAAMEGQR 240
Query: 241 RPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYRNA 300
RPSRVEALSAF+Y RFLAAI ES AQ DRLFLVCHTVNIR KL PPLPQ+AFGNYYR A
Sbjct: 241 RPSRVEALSAFIYSRFLAAINHESGAQPDRLFLVCHTVNIRQKLEPPLPQDAFGNYYRTA 300
Query: 301 MTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEIVS 360
+TLPS ETL D NCSGLVNEVRHQIAKIDAEFLRQFQEENAQLE IKATSARF+KGEIVS
Sbjct: 301 LTLPSPETLTDDNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLETIKATSARFMKGEIVS 360
Query: 361 CAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKESHMER 420
A TSLCRFPLYDADFGWG PAWAASPALPF+NLVV MD KSGDG ++A+VHL E HM +
Sbjct: 361 FALTSLCRFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAK 420
Query: 421 LEADHEFLEFASTTGS 435
LEAD EFL+FAS T S
Sbjct: 421 LEADEEFLKFASPTAS 436
BLAST of Lcy01g014450 vs. NCBI nr
Match:
XP_022151916.1 (vinorine synthase-like [Momordica charantia])
HSP 1 Score: 544.3 bits (1401), Expect = 9.7e-151
Identity = 268/436 (61.47%), Postives = 326/436 (74.77%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M +E+EV+S+E++KP SPTPA+LRR +SFLDQVT DVYNPMVY Y D F +I
Sbjct: 1 MAVEIEVISKEMIKPFSPTPAHLRRCNMSFLDQVTTDVYNPMVYFYAPTAD--FDAAKIA 60
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNC 120
+ LK+SLS VL+ +YPLAGRV+Y +F IDC+D GVPFIETRV C+L DVV FP +LN
Sbjct: 61 DHLKNSLSHVLTEFYPLAGRVNYGEFFIDCDDGGVPFIETRVNCRLSDVVGTPFPGELNK 120
Query: 121 LVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGVVEDL 180
+PFELD+L+E+SMGVQLN FECG +G+GICVSHKI DALSFF+VVNGWAA RG VE L
Sbjct: 121 FLPFELDQLEEVSMGVQLNVFECGGVGVGICVSHKIGDALSFFTVVNGWAAFGRGEVEAL 180
Query: 181 GRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEGQRRP 240
R H + LFPP ALYNTRNSI R+RVA+R+ + G+ +EAIR KYAES A E QRRP
Sbjct: 181 -RGHFASGELFPPTTTALYNTRNSIFRERVAKRYEINGANIEAIRAKYAESDAFENQRRP 240
Query: 241 SRVEALSAFVYGRFLAAIKDESSAQTDRL-------FLVCHTVNIRHKLNPPLPQNAFGN 300
SRVE+LSAF+YGRF+AA+KDE S + RL +LVCH VNIR +L PP+P+ AFGN
Sbjct: 241 SRVESLSAFLYGRFIAAVKDECSDENGRLESEKTTTYLVCHAVNIRSRLEPPVPEYAFGN 300
Query: 301 YYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVK 360
YYR+ +PS E L D NC LV VR +IAKID +L+Q Q+ L+ +K TSARF
Sbjct: 301 YYRSTFAIPSQEILDDPNCYALVPHVRQEIAKIDTHYLKQIQDNRNFLDAMKKTSARFAA 360
Query: 361 GEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKE 420
G ++SC+FTSLCR P+YDADFGWG P W SPAL F+NL V +D K GDG V+ VHLK+
Sbjct: 361 GSLLSCSFTSLCRMPIYDADFGWGKPEWVGSPALIFKNLFVLVDKKDGDG-VDVYVHLKK 420
Query: 421 SHMERLEADHEFLEFA 430
HM RLEADH FL +A
Sbjct: 421 EHMARLEADHLFLNYA 432
BLAST of Lcy01g014450 vs. TAIR 10
Match:
AT1G24430.1 (HXXXD-type acyl-transferase family protein )
HSP 1 Score: 334.7 bits (857), Expect = 1.1e-91
Identity = 179/441 (40.59%), Postives = 271/441 (61.45%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLR----RYKLSFLDQVTADVYNPMVYLYELNGDPKFTE 60
M LE+ V S+ELVKPS P NL + LSFLDQ+ ++ P ++ Y + ++
Sbjct: 1 MGLEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFY--HNKTNLSD 60
Query: 61 LEIRNRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPE 120
E + +KSSLS +L+ YYPLAGR+ + V+ CND GV F+E + C + ++ P
Sbjct: 61 KERSDHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPN 120
Query: 121 DLNCLVPFELDRLDEISMGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGV 180
+LN L PFE + ++ + VQL FFECG + LGI +SHK+ DALS VN WAA ARG
Sbjct: 121 ELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQ 180
Query: 181 VEDLGRPHLVAARLFPPRNAALYNTRNSIARDR-VARRFVVEGSKVEAIREKYAESAAME 240
+++ P A++FPP + N I ++ V RRFV S VE++RE+++ + +
Sbjct: 181 TDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKKI- 240
Query: 241 GQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYY 300
R +RVE LS F++ RF+A+ + +T +++ + H VN+R + +P +P N FGN
Sbjct: 241 ---RATRVEVLSVFIWSRFMASTNHDD--KTGKIYTLIHPVNLRRQADPDIPDNMFGNIM 300
Query: 301 RNAMTLP-SAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEEN-AQLEIIKATSARFVK 360
R ++T+P D + LV+++R +I KIDA ++++ QE+N LE + ++ FV
Sbjct: 301 RFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFVN 360
Query: 361 GEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVHLKE 420
GEIVS +FTSLC+FP+Y+ADFGWG P W AS + ++NLV F+D K GDG +EA ++L +
Sbjct: 361 GEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDG-IEAWINLDQ 420
Query: 421 SHMERLEADHEFLEFASTTGS 435
+ M R EAD E L + S+ S
Sbjct: 421 NDMSRFEADEELLRYVSSNPS 429
BLAST of Lcy01g014450 vs. TAIR 10
Match:
AT3G26040.1 (HXXXD-type acyl-transferase family protein )
HSP 1 Score: 248.8 bits (634), Expect = 7.9e-66
Identity = 146/444 (32.88%), Postives = 241/444 (54.28%), Query Frame = 0
Query: 3 LEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIRNR 62
+ V+VVS +++KPSSPTP +L+++KLS L+Q+ ++ PMV+ Y N K TE
Sbjct: 1 MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQ--LQM 60
Query: 63 LKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPEDLNCLV 122
LK SLS L+H+YPLAGR+ + IDCND G F+E RV L +++ + + L L+
Sbjct: 61 LKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLI 120
Query: 123 PFELDRLDEIS--MGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAA-AARGVVED 182
P +D ++ + + Q +FFECG++ +G+C+SHK+ADA S + WAA ++RG ++
Sbjct: 121 PTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKT 180
Query: 183 LGRPHLVAARLFPPRN-----AALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAM 242
+G P ++FPP N A + +++RF+ + S ++A++ K A+
Sbjct: 181 IGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAK----ASS 240
Query: 243 EGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNY 302
+P+RVEA+SA ++ + A + S T + ++ ++V++R +++PP +N+ GN
Sbjct: 241 FEVNQPTRVEAVSALIWKSAMKATRTVSG--TSKPSILANSVSLRSRVSPPFTKNSIGNL 300
Query: 303 YRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEII----KATSAR 362
+ + E + LV+++R + + + EII K
Sbjct: 301 V-SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDM 360
Query: 363 FVKGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALPFRNLVVFMDGKSGDGVVEALVH 422
G+ F+S CRF LY+ DFGWG P W P++ +N+V +D K G +EA V+
Sbjct: 361 IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGG-IEAWVN 420
Query: 423 LKESHMERLEADHEFLEFASTTGS 435
L E M E D E L+FAS S
Sbjct: 421 LNEQEMNLFEQDRELLQFASLNPS 433
BLAST of Lcy01g014450 vs. TAIR 10
Match:
AT1G24420.1 (HXXXD-type acyl-transferase family protein )
HSP 1 Score: 203.4 bits (516), Expect = 3.8e-52
Identity = 145/444 (32.66%), Postives = 224/444 (50.45%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPK----FTE 60
M+ VE++S E+VKPSSPTP + R LS LD +++ +Y + Y DP+ F+
Sbjct: 1 MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYA--ADPQNLLGFST 60
Query: 61 LEIRNRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRVKCKLHDVVSKSFPE 120
E +LK SLS L +YPLAGR+ ++CND+G FIE RV L + + PE
Sbjct: 61 EETSLKLKKSLSKTLPIFYPLAGRI--IGSFVECNDEGAVFIEARVDHLLSEFLKCPVPE 120
Query: 121 DLNCLVPFELDRLDEIS---MGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAA 180
L L+P E + ++ + +Q NFF CG + + ICVSHKI DA S + GWA ++
Sbjct: 121 SLELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESS 180
Query: 181 RGVVEDLGRPHLVAARLFPP-----RNAALYNTRNSIARDRVARRFVVEGSKVEAIREKY 240
RG+ L P A+ +FP + + V +RFV + SK++ +R K
Sbjct: 181 RGLGITL-IPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKA 240
Query: 241 AESAAMEGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQ 300
+ + + P+RVEA++A ++ R + + SS T R ++ VN+R K++ L +
Sbjct: 241 SRNLV----KNPTRVEAVTA-LFWRCVTKVSRLSSL-TPRTSVLQILVNLRGKVD-SLCE 300
Query: 301 NAFGNYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATS 360
N GN ++ + E A +V+E+R + + E+++
Sbjct: 301 NTIGNML--SLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEIG 360
Query: 361 ARFVKG-EIVSCAFTSLCRFPLYDADFGWGSPAWAASPALP-FRNLVVFMDGKSGDGVVE 420
+ +G E+ S C+ LYDADFGWG P W F+NL++ +D K G+G +E
Sbjct: 361 KVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEG-IE 420
Query: 421 ALVHLKESHMERLEADHEFLEFAS 431
A + L E M E D E LE AS
Sbjct: 421 AWITLTEEQMSLFECDQELLESAS 429
BLAST of Lcy01g014450 vs. TAIR 10
Match:
AT4G15390.1 (HXXXD-type acyl-transferase family protein )
HSP 1 Score: 198.7 bits (504), Expect = 9.3e-51
Identity = 138/453 (30.46%), Postives = 222/453 (49.01%), Query Frame = 0
Query: 1 MKLEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIR 60
M ++VE +S+E++KPSSPTP NL+ +LS D + VY Y N ++
Sbjct: 4 MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKN--DLISQEHTS 63
Query: 61 NRLKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRV-KCKLHDVVSKSFPEDLN 120
++LK+SLS L+ +YPLAGR+ +DC D+G F++ RV C L + + + L
Sbjct: 64 HKLKTSLSETLTKFYPLAGRI--TGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQ 123
Query: 121 CLVPFELDRLDEISMG------VQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAA 180
L+P ++ ++ V+ +F CG + +GIC++HKIADA S + + WAA A
Sbjct: 124 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 183
Query: 181 RGV--VEDLGRPHLVAARLFPPRNAALYNTRNSIA--RDRVARRFVVEGSKVEAIREKYA 240
RG + P A +PP N A + A R + +RFV E SKVE +R K A
Sbjct: 184 RGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAA 243
Query: 241 ESAAMEGQRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQN 300
++ +P+RVE+++A ++ F+A+ K + D LV N+R K+ L ++
Sbjct: 244 SEETVD---QPTRVESVTALIWKCFVASSK---TTTCDHKVLV-QLANLRSKIPSLLQES 303
Query: 301 AFGNYYRNAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQ-------LE 360
+ GN +++ L S + V ++R + ++ L + ++
Sbjct: 304 SIGNLMFSSVVL-SIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLAN 363
Query: 361 IIKATSARFVKGEIVSCAFTSLCRFPLYDADFGWGSPAWAASPALP-FRNLVVFMDGKSG 420
++ +R +S C+ PLY+A FGW SP W P NL + +D K G
Sbjct: 364 LMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDG 423
Query: 421 DGVVEALVHLKESHMERLEADHEFLEFASTTGS 435
G +EA V L E +M E + E L FA+ S
Sbjct: 424 QG-IEAFVTLPEENMSSFEQNPELLAFATMNPS 443
BLAST of Lcy01g014450 vs. TAIR 10
Match:
AT5G47950.1 (HXXXD-type acyl-transferase family protein )
HSP 1 Score: 196.8 bits (499), Expect = 3.5e-50
Identity = 141/449 (31.40%), Postives = 217/449 (48.33%), Query Frame = 0
Query: 3 LEVEVVSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYLYELNGDPKFTELEIRNR 62
++VE + +E++KPS+ TP +L +LS +D + VY Y D ++ + +
Sbjct: 4 MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFY--TKDDLISQEQTSHT 63
Query: 63 LKSSLSAVLSHYYPLAGRVDYADFVIDCNDDGVPFIETRV-KCKLHDVVSKSFPEDLNCL 122
LK+SLS +L+ ++PLAGRV+ I D+G F+E RV C L + E L L
Sbjct: 64 LKTSLSEILTKFHPLAGRVN--GVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTESLKQL 123
Query: 123 VPFELDRLDEIS-----MGVQLNFFECGAIGLGICVSHKIADALSFFSVVNGWAAAARGV 182
+P + DE + + V+ +F CG + +G+C+SH++ADA S + WAA ARG
Sbjct: 124 LPVD----DEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARGE 183
Query: 183 VEDLGRPHLVAARLFPPRNAALYNTRNSIARDRVARRFVVEGSKVEAIREKYAESAAMEG 242
+ + P + +L+P N A+ + R V +RFV SK+E +R K A
Sbjct: 184 SDLVASPDFCSTKLYPAANEAIKIPGEVVNRTSVTKRFVFVASKIEELRNKVASDVV--- 243
Query: 243 QRRPSRVEALSAFVYGRFLAAIKDESSAQTDRLFLVCHTVNIRHKLNPPLPQNAFGNYYR 302
RP+RV+++++ ++ + A S T R + N+R K+ L +N GN
Sbjct: 244 -PRPTRVQSVTSLIWKCVVTA-----STDTIREKALFQPANLRTKIPSLLSENQIGNLLF 303
Query: 303 NAMTLPSAETLADGNCSGLVNEVRHQIAKIDAEFLRQFQEENAQLEIIKATSARFVKGEI 362
N++ L DG + E ++ K + Q+E T + GEI
Sbjct: 304 NSLIL-------DGKAGMDIVETVKELQKRAEDLSGLVQDEEGS----SMTIGWRLFGEI 363
Query: 363 VSCAF----------TSLCRFPLYDADFGWGSPAWAASPALPFRNLV-VFMDGKSGDGVV 422
++ F TS C+ PLYDA FGWGSP W A P + V V +D K G G +
Sbjct: 364 INSKFNREDHDMHSVTSWCKIPLYDACFGWGSPVWVAGSVSPNLDYVTVLIDSKDGQG-I 423
Query: 423 EALVHLKESHMERLEADHEFLEFASTTGS 435
EA V L + +M LE E L FAS S
Sbjct: 424 EAWVTLPQENMLLLEQSTELLAFASPNPS 423
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
A0A2P1GIW7 | 2.0e-61 | 33.41 | Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1 | [more] |
Q70PR7 | 9.1e-59 | 33.80 | Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2 | [more] |
A0A2K8FQU5 | 7.0e-51 | 33.11 | Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... | [more] |
I3PLR4 | 2.2e-49 | 31.60 | (13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... | [more] |
Q9ZTK5 | 9.4e-48 | 31.63 | Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BB10 | 3.4e-210 | 83.91 | vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1 | [more] |
A0A6J1BS06 | 1.0e-193 | 79.13 | vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1 | [more] |
A0A6J1DG29 | 4.7e-151 | 61.47 | vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111019771 PE=3 SV=1 | [more] |
A0A0A0KPY9 | 3.5e-146 | 59.04 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1 | [more] |
A0A5D3CZ30 | 9.5e-144 | 58.58 | Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... | [more] |